Subpackaging system for cell sap

Abstract

The embodiment of the utility model discloses a subpackaging system for cell sap. The subpackaging system comprises a subpackaging pump body, a subpackaging main unit, a calibration unit and an exhaust unit which are sequentially connected in the extending direction of the same pipeline. Wherein the split charging main unit is connected to a first branch of a pipeline through a shunt valve, and comprises a plurality of split charging pieces provided with pinch valves; the calibration unit comprises a calibration container connected to the pipeline, the closed ends of the upper side and the lower side of the calibration container are obliquely arranged, and an inlet and an outlet of the calibration container are located in the lowest position and the highest position of the two closed ends of the calibration container respectively and are far away from each other. The exhaust unit comprises a gas filtering piece connected to one end of the pipeline and a diaphragm pump body. The calibration unit, the exhaust unit and the connection relation of the calibration unit and the exhaust unit in the sub-packaging system are arranged in the sub-packaging system, so that the sub-packaging precision of the sub-packaging system can be improved, the sub-packaging efficiency can be improved, meanwhile, gas residues in the preparation to be sub-packaged can be eliminated, and the finished product specification quality is higher.

Description

Technical Field

[0001] The present application relates to the technical field of preparation packaging, and in particular to a cell fluid packaging system. Background Art

[0002] In the field of cell therapy, cell fluid packaging technology is very important. First, the cell fluid must be in a sterile environment and cannot be connected to the outside world, otherwise there will be a risk of contamination. Therefore, there are closed requirements for packaging technology; secondly, there are also high requirements for packaging accuracy, including cell fluid uniformity and volume stability. In the actual packaging process, due to the airtightness requirement, peristaltic pumps are often used as the power source of the packaging system. However, the accuracy of the peristaltic pump is affected by many factors, including the accuracy of the pump tube itself, the pressure at both ends of the pump, the peristaltic pump head, the installation method and position of the pump tube, etc. It is difficult to ensure the accuracy of the peristaltic pump in the actual packaging process, resulting in a volume deviation of even 10%; secondly, since cells are relatively fragile, they need to be stored in a low-temperature environment as soon as possible after adding the preparation solution, otherwise the cells will easily die. Therefore, there are strict time limits for cell packaging, and the packaging system usually needs to have a refrigeration function to extend the packaging time and reduce cell death; finally, the current product quality also has very high requirements on the amount of residual gas after packaging. The residual gas amount will cause deviations in the cell fluid content of a single packaged product, affecting the product quality and reputation. Utility Model Content

[0003] The main purpose of the present application is to provide a cell fluid packaging system, aiming to solve the technical problems of low precision of the peristaltic pump of the existing conventional packaging system and poor product quality due to residual bubbles in the cell fluid.

[0004] To achieve the above-mentioned purpose, the present application proposes a cell fluid subpackaging system, which comprises a subpackaging pump body, a subpackaging main unit, a calibration unit and an exhaust unit connected in sequence in the extension direction of the same pipeline; wherein,

[0005] The main dispensing unit is connected to the first branch of the pipeline via a branch valve, and includes a plurality of dispensing parts provided with pinch valves;

[0006] The calibration unit comprises a calibration container connected to the pipeline; the closed ends at the upper and lower sides of the calibration container are arranged obliquely, the inlet and the outlet of the calibration container are respectively located at the lowest position and the highest position of the two closed ends of the calibration container, and the inlet and the outlet of the calibration container are far away from each other;

[0007] The exhaust unit includes a gas filter connected to one end of the pipeline and a diaphragm pump body.

[0008] Optionally, the calibration unit further comprises: a first gas detection component respectively arranged at the input port and the output port of the calibration container.

[0009] Optionally, a vertical baffle is provided on the closed end of the calibration container adjacent to the outlet.

[0010] Optionally, the exhaust unit further comprises an air pressure sensor, and the air pressure sensor is arranged at the outlet side of the gas filter.

[0011] Optionally, the filling system further comprises a second gas detector, which is arranged on the output side of the filling pump body.

[0012] Optionally, the subpackaging system further comprises a plurality of subpackaging sub-units, and the plurality of subpackaging sub-units are all connected to a plurality of second branches of the pipeline via a branch valve, and the plurality of second branches are in parallel with the first branch.

[0013] Optionally, free ends of several second branches are respectively connected to the calibration unit and the exhaust unit in sequence.

[0014] Optionally, the packaging component is a packaging bag, including a bag body, a liquid tube and puncture bodies respectively arranged on both sides of the liquid tube, the liquid tube is connected to the bag body, the puncture body is parallel to the liquid tube and penetrates into the bag body on one side close to the liquid tube to form a cavity.

[0015] Optionally, the packaging system further comprises a preparation unit, a preparation pump body and a processing unit connected in sequence, and an output end of the processing unit is connected to the other end of the pipeline.

[0016] Optionally, the exhaust unit is provided between the preparation unit and the preparation pump body.

[0017] Compared with the existing cell fluid packaging system, the packaging system of the present application has at least the following beneficial effects: by configuring the packaging system to have a packaging pump body, a packaging main unit, a calibration unit and an exhaust unit connected in sequence in the extension direction of the same pipeline, a set of trunk lines is formed to calibrate the peristaltic pump and pre-treat the mixing of bubbles in the cell fluid before packaging; specifically: the packaging main unit is connected to the first branch of the pipeline through a branch valve, and includes a plurality of packaging parts provided with a clamping valve as product parts for packaging cell fluid; the calibration unit includes a calibration container connected to the pipeline, the fixed volume of the calibration container can be used as a reference amount to calibrate the range of the peristaltic pump, and the structure and inverted connection position of the calibration container are helpful to separate the bubbles in the cell fluid; and the separated bubbles are discharged through the gas filter and the diaphragm pump body of the exhaust unit connected thereto, so that the packaging process can achieve high precision and low gas residue, thereby improving the product packaging quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying any creative work.

[0019] Figure 1 A schematic diagram of an embodiment of the packaging system of the present application;

[0020] Figure 2 This is a schematic structural diagram of an implementation scheme of a calibration container for a dispensing system of the present application;

[0021] Figure 3 It is a structural schematic diagram of an existing conventional freezing bag;

[0022] Figure 4 This is a schematic diagram of the structure of an implementation scheme of the sub-assembly of the present application;

[0023] Figure 5 This is a schematic diagram of the structure of an embodiment of the packaging system of the present application;

[0024] Reference numerals:

[0025] 1. Packing pump body; 11. Second gas detection component; 2. Packing main unit; 21. Packing component; 211. Bag body; 212. Liquid pipe; 213. Puncture body; 22. Pinch valve; 3. Calibration unit; 31. Calibration container; 311. Inlet; 312. Outlet; 313. Vertical baffle; 32. First gas detection component; 4. Exhaust unit; 41. Gas filter; 42. Diaphragm pump body; 43. Air pressure sensor; 5. Packing sub-unit; 6. Preparation unit; 7. Preparation pump body; 8. Processing unit;

[0026] The realization of the purpose, functional features and advantages of this application will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0027] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0028] It should be noted that all directional indications in the embodiments of the present invention (such as up, down, left, right, front, back...) are only used to explain the relative position relationship, movement status, etc. between the components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0029] In the present invention, unless otherwise clearly specified and limited, the terms "connection", "fixation", etc. should be understood in a broad sense. For example, "fixation" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0030] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the utility model, the descriptions of "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the meaning of "and / or" appearing in the full text includes three parallel schemes. Taking "A and / or B" as an example, it includes scheme A, or scheme B, or a scheme that satisfies both A and B. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of ordinary technicians in this field to implement. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the utility model.

[0031] Cell fluid is widely used in the field of cell therapy. At present, it is often traded and transported in the form of single-piece packaged products, which greatly improves its use efficiency. Due to the characteristics of cell fluid, the packaging technology needs to meet certain application requirements, such as avoiding contamination, uniform nutrients in the cell fluid, and small specification errors of single-piece packaged products. However, it is difficult for existing packaging technology to achieve strict precision adjustment, and it is also impossible to solve the problem that gas is inevitably mixed in during the packaging process, which affects the product specifications. Therefore, this application provides a packaging system with high precision and adjustment adaptability, and can eliminate gas residues in the product.

[0032] like Figure 1 The figure shows a cell fluid dispensing system, which comprises a dispensing pump body 1, a dispensing main unit 2, a calibration unit 3 and an exhaust unit 4 connected in sequence in the extension direction of the same pipeline; wherein,

[0033] The main dispensing unit 2 is connected to the first branch of the pipeline through a branch valve, and includes a plurality of dispensing parts 21 provided with pinch valves 22;

[0034] The calibration unit 3 comprises a calibration container 31 connected to the pipeline;

[0035] The exhaust unit 4 includes a gas filter 41 and a diaphragm pump body 42 connected to one end of the pipeline.

[0036] In this embodiment, the filling pump body 1 serves as a power source to control the flow direction of the cell fluid. A peristaltic pump commonly used in the field of liquid filling technology is usually used. The peristaltic pump pumps the fluid by alternately squeezing and releasing an elastic delivery hose, similar to pinching a hose with two fingers. As the squeezing position moves, negative pressure is formed in the tube, and the liquid flows accordingly.

[0037] The power source can be realized by other equipment such as a liquid pump, but considering the requirements of pollution-free and high-precision cell fluid packaging, the peristaltic pump is preferred in this application. As a prior art, its structure is not the improved invention point of this application and is not introduced here.

[0038] The filling main unit 2 is connected between the filling pump body 1 and the calibration unit 3, but is not connected in a direct liquid flow form, but is connected to a branch of the pipeline through a bypass valve, and is arranged in the form of a filling part 21 with a clamping valve 22.

[0039] The packaging part 21 can adopt an existing cryopreservation bag, which meets the basic requirements for cell fluid storage and is conducive to the good preservation and transportation of cell fluid.

[0040] Conventional cell packaging systems use weighing sensors as a cell packaging metering solution. The weighing sensors themselves are very accurate, but are easily affected by external factors, such as pipeline vibration, accidental touch by staff, etc. More importantly, the packaging consumables themselves are sealed, and the bag is connected to a hose. The state of the hose will change after the liquid is passed into it, which will cause great interference to the weighing. The packaging accuracy of this solution often cannot meet customer needs. Considering the connection relationship of the packaging system of this application and combining the implementation method of the peristaltic pump as the power source, this application improves the packaging pump body 1 on this basis into a system component that has a metering function in addition to providing power, as follows:

[0041] By additionally directly connecting the calibration unit 3 and the exhaust unit 4 on the pipeline, before the subpackaging, the cell fluid can be allowed to flow into the calibration unit 3 in the pipeline by closing the clamping valve 22 of the subpackaging part 21, and the gas residue in the cell fluid can be separated under the structural action of the calibration unit 3, and then the gas residue can be discharged through the exhaust unit 4. Among them, the calibration container 31 of the calibration unit 3 is a structure with a fixed volume. By utilizing the characteristic that the volume of the cell fluid in its cavity is fixed, this parameter can be associated with the subpackaging pump body 1. For example, in an embodiment where the subpackaging pump body 1 is a peristaltic pump, the number of rotations of the peristaltic pump that fills the calibration container 31 can be counted to obtain a mathematical relationship between the number of rotations and the volume of injected liquid, thereby expanding the metering function of the peristaltic pump.

[0042] Optionally, the calibration container 31 is implemented as a container with a range or scale indication function to facilitate observation when measuring the range of the peristaltic pump.

[0043] The calibration container 31 includes an inlet and an outlet, and the outlet end is connected to the gas filter 41 of the exhaust unit 4 to discharge the separated residual gas out of the pipeline. The gas filter 41 may be an air filter.

[0044] In order to achieve the separation of bubbles or residual gas in the cell fluid in the calibration container 31, the present application further provides an implementation of the calibration container 31: the closed ends on the upper and lower sides of the calibration container 31 are tilted, and the inlet 311 and the outlet 312 of the calibration container 31 are respectively located at the lowest position and the highest position of the two closed ends of the calibration container 31, and the inlet 311 and the outlet 312 of the calibration container 31 are far away from each other.

[0045] like Figure 2As shown, the calibration container 31 is composed of an upper and lower part, each of which is provided with a pipeline connection port (i.e., an inlet 311 and an outlet 312), which can cooperate with the pipeline. The two connection ports are staggered. This is because the diameter of the pipeline entering the calibration container 31 is very small, and the liquid will directly rush upward for a distance. This distance can easily exceed the height of the calibration container 31 itself. If the two connection ports are arranged in a center or overlapped manner, the liquid entering from the lower pipe port will directly enter the upper pipe port. At this time, the inside of the calibration container 31 is not filled, and the function of calibrating the flow rate of the peristaltic pump cannot be achieved. The upper container is also provided with a vertical baffle 313. The vertical baffle 313 can prevent the liquid entering from the lower pipe port from directly entering the upper pipe port along the inner surface of the upper container. Since the calibration container 31 is small in volume The inner surface of the calibration container 31 has a certain slope. When the liquid enters the calibration container 31, the space becomes larger. Since the gravity is greater than the tension, the bubbles will float to the surface of the liquid, most of the bubbles will burst, and only very small bubbles will remain. The upper and lower surfaces of the calibration container 31 are set with slopes. The slope of the upper surface can prevent the remaining bubbles from being retained at the upper end of the container and be discharged along the pipeline first, thereby ensuring that the residual bubble value inside the calibration container 31 is in a very low state, ensuring the reliability of the liquid volume. The slope of the lower surface can completely empty the internal liquid of the calibration container 31 when the liquid is drawn back from the lower end, so as to ensure that the volume of the incoming liquid and the discharged liquid is the same as the volume of the calibration container 31 itself, thereby calibrating the flow rate of the peristaltic pump.

[0046] The diaphragm pump body 42 of the exhaust unit 4 arranged at one end of the pipeline is used to discharge the separated residual gas to the outside of the filling system. The diaphragm pump body 42 is also provided with a solenoid valve for opening or closing the passage. When closed, a blockage can be formed at one end of the pipeline.

[0047] In the above embodiment, a filling system is arranged to have a filling pump body 1, a filling main unit 2, a calibration unit 3 and an exhaust unit 4 connected in sequence in the extension direction of the same pipeline to form a trunk line that can perform peristaltic pump calibration and exhaust bubble pretreatment in the cell fluid before filling; specifically: the filling main unit 2 is connected to the first branch of the pipeline through a branch valve, and includes a plurality of filling parts 21 provided with a clamping valve 22 as product parts for filling cell fluid; the calibration unit 3 includes a calibration container 31 connected to the pipeline, the fixed volume of the calibration container 31 can be used as a reference for range calibration of the peristaltic pump, and the specific structure and connection position of the calibration container 31 are helpful to separate bubbles in the cell fluid; and the separated bubbles are discharged through the gas filter 41 and the diaphragm pump body 42 of the exhaust unit 4 connected thereto, so that the filling process can achieve high precision and little or no gas residual amount, thereby improving the product filling quality.

[0048] In the aforementioned embodiment, the calibration work can be observed through the scale indication, but it is easy to produce large errors. In order to improve the calibration accuracy, based on the aforementioned embodiment, as an embodiment: the calibration unit 3 also includes a first gas detection component 32 respectively arranged at the inlet and outlet of the calibration container 31.

[0049] Specifically, the first gas detection component 32 is an air detector, which is arranged at the inlet and outlet of the calibration container 31. It can transmit a signal when gas is detected. Compared with the method of observing through a scale range, the first gas detector has the advantage of high sensitivity, which can further improve the accuracy during the process of calibrating the range of the peristaltic pump.

[0050] In some embodiments, the exhaust unit 4 further includes an air pressure sensor 43, which is disposed at the outlet side of the gas filter 41. The air pressure sensor 43 is a strain gauge or piezoresistive air pressure sensor, and by arranging the air pressure sensor 43 at the outlet side of the gas filter 41, the air tightness of the pipeline of the filling system can be detected when the diaphragm pump body 42 closes the passage.

[0051] In some embodiments, the subpackaging system further includes a second gas detector 11, which is disposed on the output side of the subpackaging pump body 1. Similarly, the second gas detector 11 is also an air detector, which is disposed on the output side of the subpackaging pump body 1 to monitor the initial residual gas of the pumped cell fluid, and thus to grasp the discharge of residual gas at other positions of the pipeline.

[0052] In some embodiments, the subpackaging system further comprises a plurality of subpackaging sub-units, each of which is connected to a plurality of second branches of the pipeline via a branch valve, and the plurality of second branches are in parallel with the first branch.

[0053] Correspondingly, the sub-packaging unit also includes a plurality of sub-packaging parts 21 provided with pinch valves 22 , and the structure and arrangement thereof may be the same as those of the main sub-packaging unit 2 .

[0054] The packaging work, especially the packaging of cell fluid, is a batch project. The packaging main unit 2 in the present application, as a group of finished packaging units, is only the simplest implementation method of the present application. In actual production, multiple groups of packaging sub-units can be set up for batch packaging, and at the same time, assisted by the connection relationship and implementation method of the calibration unit 3 and the exhaust unit 4 in the aforementioned implementation method, it is possible to achieve multi-group high-efficiency packaging and improve the packaging efficiency while obtaining the same effect as the aforementioned implementation method.

[0055] In order to obtain the same effect as in the aforementioned embodiment, specifically, the free ends of a plurality of second branches are respectively connected to the calibration unit 3 and the exhaust unit 4 in sequence.

[0056] The packaging part 21 in the above embodiment adopts the existing freezing bag, see Figure 3 The existing cryopreservation bags generally include a bag body, a bag tail, a liquid bag pipeline, and a puncture port. When liquid enters from the pipeline, the original gas in the pipeline is passed into the bag body. Since most areas of the bag body are in a fitted state, the gas preferentially enters the cavity area supported by the protective film flap of the bag body. Due to the effect of liquid tension, the liquid cannot enter the cavity area supported by the flap, and the gas is completely trapped in the two cavity areas. Even by pumping back the liquid inside the bag body, the air cannot be completely discharged. Therefore, the present application further improves the structure of the cryopreservation bag, specifically: the sub-packaging part 21 is a sub-packaging bag, including a bag body 211, a liquid tube 212 and puncture bodies 213 respectively arranged on both sides of the liquid tube 212, the liquid tube 212 is connected to the bag body 211, and the puncture body 213 is parallel to the liquid tube 212 and penetrates into the bag body 211 on the side close to the liquid tube 212 to form a cavity.

[0057] like Figure 4 As shown, in the present application, improvements are made so that the cavity in the area of ​​the liquid bag tube mouth of the cryopreservation bag and the left and right liquid bag protective membrane petals are connected as one. When viewed from the side, the upper part is bulging. When the liquid enters the bag body, the original gas in the pipeline is passed into the bag body and retained in the cavity area. Because the bag body 211 is originally in a bulging state, the liquid will not be retained in the cavity due to tension, and the liquid will flow to the bottom of the bag body 211 due to gravity. When the liquid and gas in the bag are drawn back, the gas in the cavity is discharged first, and the liquid at the bottom gradually fills the cavity area. Since this process is relatively slow, the liquid is completely filled and no gas remains, thereby achieving bubble-free packaging.

[0058] As an embodiment, based on the above-mentioned embodiment, the packaging system further includes a preparation unit 6, a preparation pump body 7 and a processing unit 8 connected in sequence, and the output end of the processing unit 8 is connected to the other end of the pipeline.

[0059] See also Figure 5 In this embodiment, the packaging system also includes a cell fluid preparation unit 6, and it is processed after the original materials are prepared. After processing, it is transferred to the packaging main unit 2, packaging sub-unit, calibration unit 3 and other components of the packaging system of this application through the packaging pump body 1, thereby forming an integrated production packaging system to improve the packaging efficiency.

[0060] Furthermore, the exhaust unit 4 is provided between the preparation unit 6 and the preparation pump body 7 to reduce the residual liquid in the cell fluid during the preparation process and further improve the quality of the packaged finished product.

[0061] The above description is only an optional embodiment of the present application, and does not limit the patent scope of the present application. All equivalent structural transformations made by using the contents of the present application specification and drawings under the inventive concept of the present application, or directly / indirectly applied in other related technical fields are included in the patent protection scope of the present application.

Claims

1. A cell fluid packaging system, characterized in that: The sub-packaging system comprises a sub-packaging pump body, a sub-packaging main unit, a calibration unit and an exhaust unit which are connected in sequence in the extending direction of the same pipeline; wherein, The main dispensing unit is connected to the first branch of the pipeline via a branch valve, and includes a plurality of dispensing parts provided with pinch valves; The calibration unit comprises a calibration container connected to the pipeline; the closed ends at the upper and lower sides of the calibration container are arranged obliquely, the inlet and the outlet of the calibration container are respectively located at the lowest position and the highest position of the two closed ends of the calibration container, and the inlet and the outlet of the calibration container are far away from each other; The exhaust unit includes a gas filter connected to one end of the pipeline and a diaphragm pump body.

2. The packaging system according to claim 1, characterized in that: The calibration unit further comprises: first gas detection components respectively arranged at the inlet and the outlet of the calibration container.

3. The packaging system according to claim 1, characterized in that: A vertical baffle is arranged on the closed end of the calibration container adjacent to the outlet.

4. The packaging system according to claim 1, characterized in that: The exhaust unit further includes an air pressure sensor, which is arranged at the outlet side of the gas filter.

5. The packaging system according to claim 1, characterized in that: The filling system further comprises a second gas detector, which is arranged at the output side of the filling pump body.

6. The packaging system according to claim 5, characterized in that: The sub-packaging system further comprises a plurality of sub-packaging sub-units, each of which is connected to a plurality of second branches of the pipeline via a branch valve, and the plurality of second branches are in parallel with the first branch.

7. The packaging system according to claim 1, characterized in that: The free ends of the plurality of second branches are respectively connected to the calibration unit and the exhaust unit in sequence.

8. The packaging system according to claim 1, characterized in that: The packaging component is a packaging bag, including a bag body, a liquid tube and puncture bodies respectively arranged on both sides of the liquid tube. The liquid tube is connected to the bag body, and the puncture body is parallel to the liquid tube and penetrates into the bag body on one side close to the liquid tube to form a cavity.

9. The packaging system according to claim 1, characterized in that: The packaging system further comprises a preparation unit, a preparation pump body and a processing unit which are connected in sequence, and the output end of the processing unit is connected to the other end of the pipeline.

10. The packaging system according to claim 9, characterized in that: The exhaust unit is arranged between the preparation unit and the preparation pump body.

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