Holographic optical tweezers device for eliminating zero-order diffraction

Abstract

The utility model discloses a holographic optical tweezers device for eliminating zero-order diffraction, and the device comprises a laser which is used for emitting a first laser beam; the beam expanding and collimating module is used for controlling the diameter of the first laser beam to obtain a third laser beam; the spatial light modulator is used for carrying out light field modulation on the third laser beam, and the modulated light field is formed after focusing; and the reflection filtering module is used for filtering zero-order diffraction light interference and adjusting a light path. Zero-order diffraction light interference is filtered through the reflection filtering module, meanwhile, the occupied space of an optical path system is effectively reduced, the problems that an optical tweezers system is too large in occupied space and inconvenient to carry in the aspect of optical path construction, an optical path is interfered by zero-order diffraction light, optical path equipment is inconvenient to finely adjust and the like are solved, and the operation process of the optical tweezers is optimized.

Description

Technical Field

[0001] The present application relates to the field of optical technology, and in particular to a holographic optical tweezers device for eliminating zero-order diffraction. Background Art

[0002] The light spot formed by the intense focus of the laser beam can be used to perform micro-manipulations such as capturing, moving and rotating particles. Such a light spot is called optical tweezers, which use laser beams to manipulate tiny objects. Traditional optical tweezers use the principles of light interference and diffraction to achieve optical operations. However, these traditional optical tweezers are usually large, complex and difficult to debug, and are not suitable for small-batch personalized production and compact equipment scenarios.

[0003] In recent years, holographic optical tweezers technology has attracted much attention. This technology uses diffraction elements such as spatial light modulators to modulate the incident light wavefront and obtain the expected light field in the focal area of ​​the objective lens to achieve the capture and manipulation of particles. Compared with traditional optical tweezers technology, holographic optical tweezers technology can not only generate a large array of point light traps with arbitrary arrangement and distribution to capture multiple particles at the same time, but also independently control each light trap through computer programming to achieve complex dynamic manipulation.

[0004] It is worth noting that in the process of modulating the light source using a spatial light modulator in the holographic optical path, zero-order diffraction light with relatively high intensity will inevitably be generated. These zero-order diffraction lights will affect the capture efficiency of the object and cause certain interference to the capture imaging. Summary of the invention

[0005] Based on the above application requirements and technical background, in order to solve the technical problem of zero-order diffraction light interference in the prior art, this application adopts the following technical solution:

[0006] In a first aspect, the present application proposes a holographic optical tweezers device for eliminating zero-order diffraction, the device comprising:

[0007] A laser, configured to emit a first laser beam;

[0008] A beam expansion and collimation module, used to control the diameter of the first laser beam to obtain a third laser beam;

[0009] A spatial light modulator, used to perform light field modulation on the third laser beam, wherein the modulated light field is formed after being focused;

[0010] The reflective filter module is used to filter out zero-order diffraction light interference and adjust the optical path.

[0011] Furthermore, the reflective filter module includes a filter, a lens assembly, a beam splitter prism, and a reflector assembly.

[0012] The filter is used to filter out the zero-order diffraction light;

[0013] The lens assembly is used to adjust the focal length and form a point light trap;

[0014] The reflector assembly includes a structure for changing and adjusting the optical path;

[0015] The beam splitter prism is used to guide the light beam.

[0016] Furthermore, the lens assembly includes a first lens, a second lens, a lens moving device and a third lens.

[0017] The first lens is disposed between the spatial light modulator and the filter, and the outgoing light of the spatial light modulator is emitted after passing through the first lens;

[0018] The second lens is arranged between the filter and the beam splitter prism, and the lens moving device is attached to the second lens and is used to control the movement of the second lens to achieve focal plane fine-tuning matching of the optical tweezers and the sample;

[0019] The third lens is arranged between the beam splitter prism and the image acquisition module.

[0020] Furthermore, the beam expansion and collimation module includes a beam expander and a collimator.

[0021] The beam expander is used to expand the diameter of the first laser beam to obtain a second laser beam, and the second laser beam is divergent;

[0022] The collimator is used to unify the straightness of the second laser beam to obtain a third laser beam, and the third laser beam is divergent.

[0023] Furthermore, the device comprises a first optical path, a second optical path and a third optical path,

[0024] The first optical path includes the laser, a beam expansion and collimation module, a spatial light modulator, a first lens, a second lens and a lens moving device;

[0025] The second optical path includes a sample and a microscope;

[0026] The third optical path includes the third lens and the image acquisition module.

[0027] Furthermore, the first laser beam emitted by the laser passes through the first optical path to obtain optical tweezers, and the optical tweezers pass through the beam splitter prism and enter the second optical path to achieve the capture and movement of the particles of the sample;

[0028] The microscope in the second optical path emits a sample light beam, and the sample light beam passes through the beam splitter prism and enters the third optical path to achieve imaging and tracking of the sample.

[0029] Furthermore, the reflector assembly includes a first reflector, which is arranged between the first lens and the filter; the reflector assembly also includes a second reflector and a third reflector, which are arranged between the filter and the second lens, and the outgoing light passing through the second reflector and the third reflector is parallel to the incident light incident on the second reflector and the third reflector and has an opposite direction.

[0030] Further, the reflector assembly further comprises a fourth reflector and a fifth reflector, wherein the fourth reflector and the fifth reflector are arranged between the beam splitter prism and the microscope, and the outgoing light passing through the fourth reflector and the fifth reflector is parallel to the incident light incident to the fourth reflector and the fifth reflector and has an opposite direction;

[0031] The reflector assembly also includes a sixth reflector and a seventh reflector, which are arranged between the third lens and the image acquisition module, and the outgoing light passing through the sixth reflector and the seventh reflector is parallel to the incident light incident to the sixth reflector and the seventh reflector and has an opposite direction.

[0032] Furthermore, the device also includes:

[0033] The image acquisition module is used to capture images and videos during the operation of holographic optical tweezers;

[0034] Microscope, used to monitor and observe the position and status of samples in real time;

[0035] The control terminal is used to control the holographic optical tweezers device, the microscope and the image acquisition module, provide a user interface and display the results of the operation.

[0036] Furthermore, the microscope comprises: an illumination light source, a stage, an objective lens, and a focusing lens.

[0037] The illumination light source is used to illuminate the sample for imaging and tracking;

[0038] The stage is used for placing the sample;

[0039] The objective lens is used to focus the light field modulated by the spatial light modulator to form optical tweezers;

[0040] The focusing mirror is used to focus the illumination light source onto the sample.

[0041] Compared with the prior art, the beneficial effects of this application are:

[0042] The holographic optical tweezers device for eliminating zero-order diffraction provided in the present application uses a reflective filter module to filter out the interference of zero-order diffraction light, while effectively reducing the space occupied by the optical path system, thereby solving the problems of the optical tweezers system occupying too much space and being inconvenient to carry in terms of optical path construction, as well as the optical path being interfered by zero-order diffraction light and the inconvenience of fine-tuning the optical path equipment, thereby optimizing the optical tweezers operation process, obtaining a dynamic analysis of the multi-dimensional structure of the sample, and achieving higher capture efficiency, capture imaging quality, and more precise optical path fine-tuning capabilities. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0044] Figure 1 This is a schematic diagram of the structure of the holographic optical tweezers device for eliminating zero-order diffraction proposed in this application;

[0045] The above drawings include the following reference numerals:

[0046] 1-laser; 2-beam expander; 3-collimator; 4-spatial light modulator; 5-first lens; 6-filter; 7-second lens; 8-beam splitter; 9-third lens; 10-image acquisition module; 11-objective lens; 12-sample; 13-focusing lens; 14-illumination light source; 15-lens moving device. DETAILED DESCRIPTION

[0047] The present application proposes a holographic optical tweezers device for eliminating zero-order diffraction. In order to describe the present application in more detail, the technical solution of the present application is described in detail below in conjunction with the accompanying drawings and specific embodiments. It should be understood that the specific implementation methods described herein are only used to explain the present application and are not used to limit the present application. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present application.

[0048] When used in practical applications, specifically, on the one hand, the laser 1 emits the laser used for light capture, which enters the beam expansion and collimation module including the beam expander 2 and the collimator 3, and the beam expansion and collimation module expands the laser emitted by the laser and unifies the straightness. The beam after beam expansion and collimation is irradiated on the spatial light modulator 4 for light field modulation, and then the beam passes through the first lens 5 and the second lens 7, wherein the second lens 7 can move forward and backward along the light path direction with the lens displacement device 15. A filter 6 is placed between the first lens 5 and the second lens 7 to filter out the zero-order diffraction light interference. Then the light beam passes through the beam splitter prism 8 and is converged on the sample 12 by the objective lens 11 of the microscope to achieve the capture and movement of the sample particles. On the other hand, the illumination light source 14 emits a white light source, and the illumination light is irradiated and converged on the sample 12 through the focusing lens 13 of the microscope, and then refracted by the beam splitter prism 8, passes through the third lens 9, and the image acquisition module 10 receives the light intensity to image and track the sample.

[0049] The holographic optical tweezers device proposed in this application covers multiple fields in practical applications. In the biomedical field, this application is used to manipulate and study biological cells, microorganisms and molecules without contact, including the operation and study of single cells, and the three-dimensional construction of tissues and cells; in the field of micro-nanotechnology, this application is used to manipulate and assemble nanoparticles and nanostructures, and then realize the preparation and research of nanodevices, including the assembly of nanomaterials, the manufacture of nanodevices and the construction of nanostructures; in the field of optical communications, this application is used to connect and adjust optical fibers in optical fiber communication systems, realize precise alignment and correction of optical fiber end faces, and improve the transmission efficiency and quality of optical signals, including automatic adjustment of optical fiber connections, optimization of optical fiber end faces and performance improvement of optical fiber communication systems; in the field of display technology, this application is used for three-dimensional display and precise control of light fields, including three-dimensional projection display, holographic image display and spatial manipulation in virtual reality technology; this application can also be applied to materials science, chemical reactions, optical sensing, photon computing and quantum information.

[0050] In a first aspect, the present application proposes a holographic optical tweezers device for eliminating zero-order diffraction, and the structural schematic diagram of the device is as follows: Figure 1 As shown, the device comprises:

[0051] Laser 1, used for emitting a first laser beam;

[0052] A beam expansion and collimation module, used to control the diameter of the first laser beam to obtain a third laser beam;

[0053] A spatial light modulator 4 is used to perform light field modulation on the third laser beam, and the modulated light field is formed after focusing;

[0054] The reflective filter module is used to filter out zero-order diffraction light interference and adjust the optical path.

[0055] Furthermore, the reflective filter module includes a filter 6, a lens assembly, a beam splitter prism 8, and a reflector assembly.

[0056] The filter 6 is used to filter out the zero-order diffraction light;

[0057] The lens assembly is used to adjust the focal length and form a point light trap;

[0058] The reflector assembly includes a structure for changing and adjusting the optical path;

[0059] The beam splitter prism 8 is used to guide the light beam.

[0060] Among them, the zero-order diffraction light is one of the main optical phenomena caused by diffraction of light waves passing through the edge of an object, a pore or the edge of an optical element in an optical system. The "zero-order" in the name of this diffraction light indicates that the main order of diffraction is the zero-order, that is, it is the brightest part in the center of the object. The zero-order diffraction light may cause background noise, reduce imaging quality or interfere with the operation of precise manipulation of particles. Therefore, the control and management of zero-order diffraction light is crucial in holographic optical tweezers technology. The filter 6 can filter out the zero-order diffraction light by selectively transmitting or blocking light of a specific wavelength, thereby improving the imaging clarity and manipulation accuracy of the system. Optionally, the zero-order diffraction light can also be filtered out by a spatial isolator.

[0061] The lens assembly adjusts the position and properties of the lens to adjust the focal length, forming a point light trap, and providing the necessary optical environment for manipulating particles.

[0062] The reflector assembly adjusts the direction of the light path through reflection and refraction to ensure that the various components of the optical system can work together effectively. The design of the reflector can affect the stability and performance of the optical system.

[0063] The beam splitter prism 8 is used to separate and guide light beams, ensuring that light of different wavelengths can be correctly guided to corresponding optical paths, which helps the division of labor and cooperation of the optical system.

[0064] Furthermore, the lens assembly includes a first lens 5, a second lens 7, a lens moving device 15 and a third lens 9.

[0065] The first lens 5 is disposed between the spatial light modulator 4 and the filter 6, and the outgoing light of the spatial light modulator 4 is emitted after passing through the first lens 5;

[0066] The second lens 7 is arranged between the filter 6 and the beam splitter prism 8, and the lens moving device 15 is attached to the second lens 7 and is used to control the movement of the second lens 7 to achieve focal plane fine-tuning matching of the optical tweezers and the sample 12;

[0067] The third lens 9 is disposed between the beam splitter prism 8 and the image acquisition module 10 .

[0068] Among them, through the lens assembly, fine-tuning of the focus and height manipulation of the sample 12 can be achieved. The position and design of the first lens 5 affect the focusing of the outgoing light and the light field modulation effect. The second lens 7 is fine-tuned through the lens moving device 15 to achieve precise control of the focus, ensuring that the optical system can maintain a good focus when manipulating the sample 12; the design of the third lens 9 affects the clarity and quality of the final image, ensuring that the image acquisition module 10 can obtain high-quality image data.

[0069] Furthermore, the beam expansion and collimation module includes a beam expander 2 and a collimator 3, the beam expander 2 is used to expand the diameter of the first laser beam to obtain a second laser beam, and the second laser beam is divergent; the collimator 3 is used to unify the straightness of the second laser beam to obtain a third laser beam, and the third laser beam is divergent.

[0070] Among them, the diameter of the laser beam is enlarged by the beam expander 2 to produce a divergent beam. This design is intended to provide a sufficient beam diameter for subsequent operations so as to perform precise optical modulation on components such as the spatial light modulator 4; the direction of the beam is adjusted by the collimator 3 to ensure that the light rays in the beam are basically parallel, which helps to maintain the stability of the beam in subsequent optical components and provides a good foundation for fine spatial light modulation and focusing operations.

[0071] Furthermore, the device comprises a first optical path, a second optical path and a third optical path,

[0072] The first optical path includes the laser 1, a beam expansion and collimation module, a spatial light modulator 4, a first lens 5, a second lens 7 and a lens moving device 15;

[0073] The second optical path includes a sample 12 and a microscope;

[0074] The third optical path includes the third lens 9 and the image acquisition module 10 .

[0075] Among them, the first laser beam emitted by the laser 1 passes through the first optical path to obtain optical tweezers, and the optical tweezers pass through the dichroic prism 8 to enter the second optical path, so as to grasp and move the particles of the sample 12; the microscope in the second optical path emits a sample 12 beam, and the sample 12 beam passes through the dichroic prism 8 to enter the third optical path, so as to image and track the sample 12.

[0076] Among them, the first optical path is designed to generate optical tweezers and control optical tweezers for grabbing and moving particles, the second optical path is designed to observe, image and track the grabbed and moved particles, and the third optical path is designed to image and track the sample 12 in more detail.

[0077] In one embodiment, the reflector assembly includes a first reflector, which is arranged between the first lens 5 and the filter 6; the reflector assembly also includes a second reflector and a third reflector, which are arranged between the filter 6 and the second lens 7, and the outgoing light passing through the second reflector and the third reflector is parallel to the incident light incident on the second reflector and the third reflector and has an opposite direction;

[0078] The reflector assembly further includes a fourth reflector and a fifth reflector, wherein the fourth reflector and the fifth reflector are arranged between the beam splitter prism 8 and the microscope, and the outgoing light passing through the fourth reflector and the fifth reflector is parallel to the incident light incident to the fourth reflector and the fifth reflector and has an opposite direction;

[0079] The reflector assembly also includes a sixth reflector and a seventh reflector, which are arranged between the third lens 9 and the image acquisition module 10, and the outgoing light passing through the sixth reflector and the seventh reflector is parallel to the incident light incident to the sixth reflector and the seventh reflector and has an opposite direction.

[0080] Among them, through the reasonable configuration and arrangement of the seven reflectors in the reflector assembly, the light can be reflected multiple times inside the device, thereby completing a specific optical path and reducing the space occupied by the device. By cleverly arranging and reflecting the light path, the present application can achieve the required functions in a limited space, improve the compactness and efficiency of the optical system, and such a design is very valuable for some application scenarios with limited space and requiring high optimization.

[0081] Furthermore, the device also includes:

[0082] The image acquisition module 10 is used to capture images and videos during the operation of holographic optical tweezers;

[0083] A microscope for monitoring and observing the position and state of the sample 12 in real time;

[0084] The control terminal is used to control the holographic optical tweezers device, the microscope and the image acquisition module 10, provide a user interface and display the results of the operation.

[0085] In one embodiment, the image acquisition module 10 includes a CCD camera (Charge-Coupled Device Camera). A CCD camera is an image sensor device using charge-coupled device technology, commonly used in optical and digital imaging applications, and has the characteristics of high sensitivity, low noise, and linear response. A CCD camera can capture images under weak light conditions, and has a high signal-to-noise ratio and resolution. It can capture subtle details and tiny structures, which is very beneficial for improving imaging quality and reducing interference. A CCD camera can capture images at a high frame rate, allowing real-time monitoring and tracking, providing high-quality, high-sensitivity image data for holographic optical tweezers, and providing a reliable foundation for the stability and performance of the system.

[0086] Optionally, the image acquisition module 10 may include a CMOS camera (Complementary Metal-Oxide-Semiconductor), an EMCCD camera (Electron-Multiplying Charge-Coupled Device), a PMOS camera (Photomultiplier Tube), etc. for collecting images or videos.

[0087] Optionally, the image acquisition module 10 may also include a sensor interface, a data transmission interface and a power management component. The sensor interface is used to connect and control other sensor devices to obtain environmental information related to image acquisition; the data transmission interface is used to provide an interface for transmitting image data to other devices or systems (such as a control terminal); the power management component is used to provide power to ensure the normal operation of the image acquisition module 10.

[0088] The type of microscope depends on the research objectives, the properties of the sample 12, and the required imaging characteristics. For example, the most common optical microscope is suitable for observing samples 12 in the visible light range; fluorescence microscopes can be used to mark and track biomolecules, and highly selective imaging of specific molecules or structures can be achieved by exciting fluorescent dyes in the sample 12 and detecting the emitted fluorescence signals; and confocal microscopes use laser light sources to obtain clear images at different depths of the sample 12, thereby providing deep imaging, which is very useful for observing thicker samples 12 or performing three-dimensional imaging.

[0089] The control terminal is generally a computer or an embedded system, equipped with corresponding software and hardware to realize comprehensive control and user interaction of the holographic optical tweezers system. Optionally, the control terminal may include a user interaction unit, an image processing unit, a system control unit, a data display and analysis unit, a remote control unit, etc.

[0090] The user interaction unit provides an interface that enables the user to interact with the holographic optical tweezers device, including a graphical user interface, a touch screen, buttons, menus, etc. The user can use this unit to set experimental parameters, start experiments, adjust settings, etc.; the image processing unit is used to process and optimize the collected images, which may include image processing operations such as feature extraction, denoising, contrast enhancement, and brightness adjustment; the system control unit is used to control all components as a whole, and the instructions input by the user through the user interaction unit are converted by the system control unit into operations on various parts of the device system; the data display and analysis unit is used to display the images collected by the image acquisition module 10 on the control terminal, and at the same time provides basic data analysis functions. The user can use this unit to view the experimental results in real time, perform preliminary analysis, or save data for subsequent processing; the remote control unit allows the user to remotely control and monitor the holographic optical tweezers device through the network or other remote means, which is very useful for cross-regional collaboration or remote access.

[0091] Furthermore, the microscope comprises: an illumination light source 14, a stage, an objective lens 11, and a focusing lens 13.

[0092] The illumination light source 14 is used to illuminate the sample 12 for imaging and tracking;

[0093] The stage is used to place the sample 12;

[0094] The objective lens 11 is used to focus the light field modulated by the spatial light modulator 4 to form optical tweezers;

[0095] The focusing lens 13 is used to focus the illumination light source 14 onto the sample 12 .

[0096] Among them, optionally, the illumination light source 14 can be a white light LED, a laser diode (LED) or other light source types, with adjustable spectral characteristics to meet the imaging requirements of different samples 12; the intensity of the illumination light source 14 can be adjustable to adapt to the brightness requirements of different samples 12 while avoiding excessive illumination of the biological sample 12.

[0097] Optionally, the stage may have XYZ axis mobility so that the user can accurately locate and adjust the position of the sample 12 ; for biological samples 12 , the stage may have a temperature control function to maintain ideal growth or experimental conditions for the sample 12 .

[0098] Among them, optionally, the objective lens 11 includes objective lenses 11 of different magnifications to meet the needs of different observation scales; the objective lens 11 can be coated with a special anti-reflection coating or a fluorescent coating to optimize the imaging quality or adapt to the application of fluorescence microscopy.

[0099] Among them, optionally, the focusing mirror 13 can be equipped with an autofocus system to ensure that the optimal focus is maintained during the experiment, especially when performing real-time monitoring and long-term observation; the focusing mirror 13 can be of adjustable focal length so that the user can adjust the focusing depth of the optical tweezers according to experimental requirements.

[0100] The above description is only a preferred embodiment of the present application and is not intended to limit the present application. Those skilled in the art should be aware that there may be many examples based on the basic method principles provided by the present application in combination with actual conditions, and all of them should be within the scope of protection of the present application without sufficient creative work.

[0101] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms are not necessarily for the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0102] It should also be noted that, in this specification, relational terms such as first and second, etc. are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "comprises", "comprising" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

Claims

1. A holographic optical tweezers device for eliminating zero-order diffraction, characterized in that: The device comprises: A laser, configured to emit a first laser beam; A beam expansion and collimation module, used to control the diameter of the first laser beam to obtain a third laser beam; A spatial light modulator, used to perform light field modulation on the third laser beam, wherein the modulated light field is formed after being focused; The reflective filter module is used to filter out zero-order diffraction light interference and adjust the optical path.

2. The device according to claim 1, characterized in that The reflective filter module includes a filter, a lens assembly, a beam splitter prism, and a reflector assembly. The filter is used to filter out the zero-order diffraction light; The lens assembly is used to adjust the focal length and form a point light trap; The reflector assembly includes a structure for changing and adjusting the optical path; The beam splitter prism is used to guide the light beam.

3. The device according to claim 2, characterized in that The lens assembly includes a first lens, a second lens, a lens moving device and a third lens, The first lens is disposed between the spatial light modulator and the filter, and the outgoing light of the spatial light modulator is emitted after passing through the first lens; The second lens is arranged between the filter and the beam splitter prism, and the lens moving device is attached to the second lens and is used to control the movement of the second lens to achieve focal plane fine-tuning matching of the optical tweezers and the sample; The third lens is arranged between the beam splitter prism and the image acquisition module.

4. The device according to any one of claims 1 to 3, characterized in that: The beam expansion and collimation module includes a beam expander and a collimator. The beam expander is used to expand the diameter of the first laser beam to obtain a second laser beam, and the second laser beam is divergent; The collimator is used to unify the straightness of the second laser beam to obtain a third laser beam, and the third laser beam is divergent.

5. The device according to any one of claims 1 to 3, characterized in that: The device comprises a first optical path, a second optical path and a third optical path, The first optical path includes the laser, a beam expansion and collimation module, a spatial light modulator, a first lens, a second lens and a lens moving device; The second optical path includes a sample and a microscope; The third optical path includes the third lens and the image acquisition module.

6. The device according to claim 5, characterized in that The first laser beam emitted by the laser passes through the first optical path to obtain optical tweezers, and the optical tweezers pass through the beam splitter prism and enter the second optical path to achieve the capture and movement of the particles of the sample; The microscope in the second optical path emits a sample light beam, and the sample light beam passes through the beam splitter prism and enters the third optical path to achieve imaging and tracking of the sample.

7. The device according to any one of claims 1 to 3, characterized in that: The reflector assembly includes a first reflector, which is arranged between the first lens and the filter; the reflector assembly also includes a second reflector and a third reflector, which are arranged between the filter and the second lens, and the outgoing light passing through the second reflector and the third reflector is parallel to the incident light incident on the second reflector and the third reflector and has an opposite direction.

8. The device according to any one of claims 1 to 3, characterized in that: The reflector assembly further includes a fourth reflector and a fifth reflector, wherein the fourth reflector and the fifth reflector are arranged between the beam splitter prism and the microscope, and the outgoing light passing through the fourth reflector and the fifth reflector is parallel to the incident light incident to the fourth reflector and the fifth reflector and has an opposite direction; The reflector assembly also includes a sixth reflector and a seventh reflector, which are arranged between the third lens and the image acquisition module, and the outgoing light passing through the sixth reflector and the seventh reflector is parallel to the incident light incident to the sixth reflector and the seventh reflector and has an opposite direction.

9. The device according to any one of claims 1 to 3, characterized in that: The device also includes: The image acquisition module is used to capture images and videos during the operation of holographic optical tweezers; Microscope, used to monitor and observe the position and status of samples in real time; The control terminal is used to control the holographic optical tweezers device, the microscope and the image acquisition module, provide a user interface and display the results of the operation.

10. The device according to claim 9, characterized in that The microscope comprises: an illumination light source, a stage, an objective lens, and a focusing lens. The illumination light source is used to illuminate the sample for imaging and tracking; The stage is used to place the sample; The objective lens is used to focus the light field modulated by the spatial light modulator to form optical tweezers; The focusing mirror is used to focus the illumination light source onto the sample.

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