Outdoor water eDNA collecting and filtering membrane

Abstract

The utility model belongs to the technical field of water body detection, and particularly relates to an outdoor water body eDNA collection filter membrane which comprises a protective layer, a plurality of connecting lugs are fixedly connected to the outer side of the protective layer, the connecting lugs are evenly distributed on the outer side of the protective layer, a slow flow layer is arranged in the protective layer, a pretreatment layer is arranged above the protective layer, and the pretreatment layer is arranged on the outer side of the protective layer. Three step-by-step filtering membranes are arranged in the protection layer, the three step-by-step filtering membranes are all located below the flow slowing layer and are sequentially arranged from top to bottom according to the internal pore size, a reinforcing layer is arranged below the protection layer, and a rubber friction ring is fixedly connected to the outer side of the reinforcing layer; through cooperation of the step-by-step filtering membrane, the reinforcing layer and other components, when eDNA in outdoor water is collected and filtered, classified collection can be performed according to the particle size of the eDNA, damage caused by the fact that a water sample exerts large pressure on the step-by-step filtering membrane can be avoided, and the eDNA collecting and filtering effect is guaranteed.

Description

Technical Field

[0001] The utility model belongs to the technical field of water body detection, and in particular relates to an outdoor water body eDNA collection filter membrane. Background Art

[0002] eDNA refers to environmental DNA, which refers to DNA molecules released into water, soil or air by various organisms (including microorganisms, plants and animals) existing in the natural environment. The outdoor water eDNA collection filter membrane is a filter used to collect environmental DNA (eDNA). Through this membrane, researchers can block and filter water samples collected in natural water bodies, thereby collecting microbial DNA, plant DNA or animal DNA in the water body, etc., which can be used for environmental monitoring, biodiversity surveys and population research. In actual applications, staff will choose eDNA collection filter membranes with different pore sizes according to different types of samples and research needs, but this single pore spacing filter membrane will block all eDNA molecules with particle diameters larger than the pore size, resulting in the filter membrane surface blocking the collection of eDNA molecules of various particle sizes, and the water sample will exert a large pressure on the membrane when it contacts the membrane surface, resulting in changes in the internal pore size of the membrane, which reduces the collection and filtration effect of eDNA, and thus is not convenient for the staff's subsequent research work. Utility Model Content

[0003] The utility model provides an outdoor water body eDNA collection filter membrane, which has the characteristics of being able to separate and collect molecules of different particle sizes when filtering and collecting eDNA molecules in the water body.

[0004] The utility model provides the following technical solution: it includes a protective layer, a plurality of connecting ears are fixedly connected to the outside of the protective layer, and the plurality of connecting ears are evenly distributed on the outside of the protective layer, a slow flow layer is arranged inside the protective layer, a pretreatment layer is arranged above the protective layer, three step-by-step filtering membranes are arranged inside the protective layer, the three step-by-step filtering membranes are all located below the slow flow layer, and the three step-by-step filtering membranes are arranged in sequence from top to bottom according to the internal pore size, a reinforcing layer is arranged below the protective layer, and a rubber friction ring is fixedly connected to the outside of the reinforcing layer.

[0005] Wherein, an adhesive layer is bonded between the protective layer, the slow flow layer and the three step-by-step filtration membranes.

[0006] Wherein, a plurality of connection protrusions are fixedly connected between the protection layer and the pretreatment layer.

[0007] Wherein, the tops of the three step-by-step filtration membranes are all provided with a hydrophilic layer.

[0008] Among them, a plurality of distribution protrusions are fixedly connected between the reinforcement layer and the protection layer, and the plurality of distribution protrusions are evenly distributed between the protection layer and the reinforcement layer.

[0009] The beneficial effect of the utility model is that through the cooperation of components such as the step-by-step filtration membrane and the reinforcement layer, when collecting and filtering eDNA in outdoor water bodies, the eDNA can be classified and collected according to the particle size, and the water sample can be prevented from exerting a large pressure on the step-by-step filtration membrane to cause damage, thereby ensuring the collection and filtration effect of eDNA.

[0010] The parts not involved in the device are the same as those in the prior art or can be implemented by using the prior art. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 It is a three-dimensional structural schematic diagram of the utility model;

[0012] Figure 2 It is a schematic diagram of the cross-sectional structure of the utility model;

[0013] Figure 3 for Figure 2 The enlarged schematic diagram of point A in the middle;

[0014] Figure 4 for Figure 2 A magnified schematic diagram of point B in the middle.

[0015] In the figure: 1. Protective layer; 11. Connecting ear; 12. Slow flow layer; 13. Adhesive layer; 2. Pretreatment layer; 21. Connecting bump; 3. Step-by-step filtration membrane; 31. Hydrophilic layer; 4. Reinforcement layer; 41. Rubber friction ring; 42. Sharing bump. DETAILED DESCRIPTION

[0016] See also Figure 1-Figure 4 The utility model provides the following technical solutions: including a protective layer 1, a plurality of connecting ears 11 are fixedly connected to the outside of the protective layer 1, and the plurality of connecting ears 11 are evenly distributed on the outside of the protective layer 1, a slow flow layer 12 is arranged inside the protective layer 1, a pretreatment layer 2 is arranged above the protective layer 1, and three step-by-step filter membranes 3 are arranged inside the protective layer 1, the three step-by-step filter membranes 3 are all located below the slow flow layer 12, and the three step-by-step filter membranes 3 are arranged in sequence from top to bottom according to the internal pore size, a reinforcing layer 4 is arranged below the protective layer 1, and a rubber friction ring 41 is fixedly connected to the outside of the reinforcing layer 4.

[0017] In this implementation scheme: the protective layer 1 is used to support and connect the various components, so as to complete the filtering and collection of eDNA in outdoor water bodies. The staff can take water samples from outdoors through sampling tubes, etc., and then the staff can install the protective layer 1 in a suitable container, and then the staff can introduce the water sample into the container. Since the protective layer 1 is water-permeable, the water sample can flow from the inside of the protective layer 1. Several connecting ears 11 fixed on the outside of the protective layer 1 are used to assist the installation of the protective layer 1 inside the container. The staff can clamp the connecting ears 11 through the container, so that the protective layer 1 can be stably fixed on the inside of the container, thereby ensuring that the water sample can contact the top of the protective layer 1, and the protective layer 1 has a certain ductility, and the water sample can be The protective layer 1 flows inside so that the water sample can reach the inside of the protective layer 1, thereby contacting the step-by-step filtration membrane 3 inside the protective layer 1. There are three step-by-step filtration membranes 3, and the three step-by-step filtration membranes 3 are arranged from top to bottom according to the internal pore size. When the staff installs the protective layer 1 inside the container, it is necessary to ensure that the step-by-step filtration membrane 3 with the largest pore size is located at the top, so that when the water sample contacts the step-by-step filtration membranes 3 in turn, the larger particles of eDNA will be filtered and blocked by the top step-by-step filtration membrane 3, while the smaller eDNA will flow downward and contact other step-by-step filtration membranes 3. Since there are certain gaps between the three step-by-step filtration membranes 3, it is ensured that the water sample can fully contact different step-by-step filtration membranes 3, thereby achieving the effect of filtering eDNA according to the particle size. In order to achieve the purpose of filtering and blocking respectively, a slow flow layer 12 is installed inside the protective layer 1, and the slow flow layer 12 is located above the step-by-step filtration membrane 3. The slow flow layer 12 has a lower water permeability, so that the flow speed of the water sample will be slowed down, thereby preventing the water sample from exerting a large pressure on the step-by-step filtration membrane 3, thereby ensuring the collection and filtering effect of the step-by-step filtration membrane 3 on the eDNA in the water sample. When the water sample contacts the slow flow layer 12 and is blocked by the slow flow layer 12 to slow down the movement speed, the slow flow layer 12 will be subjected to a large pressure from the water sample, and at this time the protective layer 1 will produce a certain deformation, thereby preventing the water source from exerting too much pressure on the slow flow layer 12, causing the slow flow layer 12 to be damaged, thereby ensuring the filtering and blocking effect of the step-by-step filtration membrane 3 on the eDNA molecules, and a pretreatment layer 2 is provided above the protective layer 1. The pretreatment layer 2 is provided with relatively large holes inside, so that large particles of impurities in the water source will be blocked by the pretreatment layer 2 in advance, preventing the large particles of impurities from contacting the protective layer 1 and the step-by-step filtration membrane 3, causing damage to the surface of the step-by-step filtration membrane 3, and ensuring that the step-by-step filtration membrane 3 can stably filter and collect eDNA in the water sample. The reinforcement layer 4 provided below the protective layer 1 is used to limit the deformation amplitude of the protective layer 1. A rubber friction ring 41 is fixed on the outside of the reinforcement layer 4. The rubber friction ring 41 will contact the inner wall of the container, so that the rubber friction ring 41 can avoid the movement of the reinforcement layer 4 through the friction between the rubber friction ring 41 and the container, ensuring that the reinforcement layer 4 can stably support the protective layer 1, and avoid the protective layer 1 from being greatly deformed and causing surface damage.The reinforcing layer 4 is a rigid structure and also has holes inside, so that after the water sample completes the collection and filtration operation, when it contacts the reinforcing layer 4, it can flow downward from the inside of the reinforcing layer 4 to the inside of the container, thereby completing the collection and filtration operation of eDNA in outdoor water bodies.

[0018] An adhesive layer 13 is bonded between the protective layer 1, the slow flow layer 12 and the three step-by-step filtration membranes 3; an adhesive layer 13 is connected between the protective layer 1, the slow flow layer 12 and the step-by-step filtration membranes 3 so that when the water sample contacts the slow flow layer 12, the protective layer 1 can produce a certain amount of deformation to prevent the slow flow layer 12 from being damaged due to excessive pressure from the water source, thereby ensuring that the slow flow layer 12 can buffer the flow velocity of the water source and ensure the filtering and blocking effect of the step-by-step filtration membrane 3 on eDNA molecules.

[0019] A plurality of connecting protrusions 21 are fixedly connected between the protective layer 1 and the pretreatment layer 2; the connecting protrusions 21 fixed between the protective layer 1 and the pretreatment layer 2 are used to connect the pretreatment layer 2 and the protective layer 1, so that the water source will first contact the pretreatment layer 2 before contacting the protective layer 1, and then the pretreatment layer 2 can block large particles of impurities in the water sample in advance, preventing the large particles of impurities from contacting the inside of the protective layer 1, causing damage to the surface of the protective layer 1, and destroying the overall stability of the protective layer 1.

[0020] A hydrophilic layer 31 is provided on the top of each of the three step-by-step filtration membranes 3; the hydrophilic layer 31 provided on the top of the three step-by-step filtration membranes 3 is used to increase the hydrophilicity of the surface of the step-by-step filtration membrane 3. The hydrophilic layer 31 can be made of PEG or polyethyleneimine, which is covered on the surface of the step-by-step filtration membrane 3, and can effectively increase the hydrophilicity of the step-by-step filtration membrane 3, thereby achieving the purpose of improving the filtering and collection effect and efficiency of the step-by-step filtration membrane 3 on eDNA in water samples.

[0021] A plurality of sharing protrusions 42 are fixedly connected between the reinforcing layer 4 and the protective layer 1, and the plurality of sharing protrusions 42 are evenly distributed between the protective layer 1 and the reinforcing layer 4; the sharing protrusions 42 fixed between the reinforcing layer 4 and the protective layer 1 are used to share the force applied by the protective layer 1 to the sharing protrusions 42, thereby preventing the protective layer 1 from transmitting the pressure to the reinforcing layer 4 through the sharing protrusions 42 when supporting the slow flow layer 12 to withstand the pressure of the water source, thereby enabling the reinforcing layer 4 to achieve the purpose of supporting and limiting the protective layer 1 through the friction between the rubber friction ring 41 and the container, thereby preventing the protective layer 1 from undergoing large deformation and causing surface damage.

[0022] The working principle and use process of the utility model are as follows: when the staff needs to collect and filter the eDNA in the outdoor water body, the staff can first sample the outdoor water body through the sampling tube, and then the staff can install the protective layer 1 on the inside of a suitable container, and squeeze and clamp the connecting ear 11 of the container to make the protective layer 1 firmly installed on the inside of the container, and then the staff can introduce the water sample into the container so that the water sample contacts the protective layer 1. Before the water sample contacts the protective layer 1, it will first contact the pretreatment layer 2 above the protective layer 1. At this time, the pretreatment layer 2 will block the large particles of impurities in the water sample to prevent the large particles of impurities from contacting the protective layer 1. Protective layer 1, since the pretreatment layer 2 has holes inside, the water sample can flow through the pretreatment layer 2 to the top of the protective layer 1, and the protective layer 1 is permeable, so that the water source will reach the inside of the protective layer 1 and contact the slow flow layer 12, and the slow flow layer 12 has low water permeability, so that the slow flow layer 12 will slow down the flow speed of the water sample, so that the contact time between the water sample and the step-by-step filtration membrane 3 can be guaranteed, and the surface of the step-by-step filtration membrane 3 has a hydrophilic layer 31, thereby ensuring the filtering and collection effect of the step-by-step filtration membrane 3 on the eDNA in the water sample, and the step-by-step filtration membrane 3 has three, and the three step-by-step filtration membranes 3 are arranged from top to bottom according to the internal pore size, so that the water sample will contact respectively The three step-by-step filtration membranes 3 enable the eDNA of different particle sizes in the water sample to be collected and filtered by different step-by-step filtration membranes 3, respectively, to complete the purpose of collecting eDNA of particles of different sizes. After the water source flows through the step-by-step filtration membrane 3, it reaches the surface of the reinforcing layer 4, and flows into the container through the holes in the reinforcing layer 4. When the protective layer 1 and the slow flow layer 12 come into contact with the water sample, the slow flow layer 12 will slow down the flow speed of the water sample, so that the slow flow layer 12 will be subjected to greater pressure. At this time, the slow flow layer 12 will transfer the pressure to the protective layer 1, and the protective layer 1 will produce a certain deformation to prevent the slow flow layer 12 from being damaged due to the greater pressure, and the protective layer The pressure borne by the protective layer 1 will be distributed to the reinforcing layer 4 through the distribution protrusion 42. The rubber friction ring 41 fixed on the outside of the reinforcing layer 4 has friction with the inner wall of the container, so that the rubber friction ring 41 can support the reinforcing layer 4, thereby limiting the deformation range of the protective layer 1, avoiding the protective layer 1 from being damaged due to a large deformation, and thus ensuring that the step-by-step filtration membrane 3 inside the protective layer 1 can stably collect and filter the eDNA in the water sample. After the collection and filtration operation is completed, the staff can scratch the surface of the protective layer 1, thereby taking out the step-by-step filtration membrane 3 from the inside of the protective layer 1, and conducting research and analysis on the eDNA collected by the step-by-step filtration membrane 3.

Claims

1. An outdoor water eDNA collection filter membrane, comprising a protective layer (1), characterized in that: The outer side of the protective layer (1) is fixedly connected with a plurality of connection ears (11), and the plurality of connection ears (11) are evenly distributed on the outer side of the protective layer (1). A slow flow layer (12) is provided inside the protective layer (1). A pretreatment layer (2) is provided above the protective layer (1). Three step-by-step filtering membranes (3) are provided inside the protective layer (1), and the three step-by-step filtering membranes (3) are all located below the slow flow layer (12). The three step-by-step filtering membranes (3) are arranged in order from top to bottom according to the size of the internal pore size. A reinforcement layer (4) is provided below the protective layer (1), and a rubber friction ring (41) is fixedly connected to the outer side of the reinforcement layer (4).

2. The outdoor water eDNA collection filter membrane according to claim 1, characterized in that: An adhesive layer (13) is bonded between the protective layer (1), the slow flow layer (12) and the three step-by-step filtration membranes (3).

3. The outdoor water eDNA collection filter membrane according to claim 1, characterized in that: A plurality of connection protrusions (21) are fixedly connected between the protective layer (1) and the pretreatment layer (2).

4. The outdoor water eDNA collection filter membrane according to claim 1, characterized in that: The tops of the three progressive filtration membranes (3) are all provided with a hydrophilic layer (31).

5. The outdoor water eDNA collection filter membrane according to claim 1, characterized in that: A plurality of distribution protrusions (42) are fixedly connected between the reinforcement layer (4) and the protective layer (1), and the plurality of distribution protrusions (42) are evenly distributed between the protective layer (1) and the reinforcement layer (4).

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