Efficient cell separation device

Abstract

The utility model discloses an efficient cell separation device which comprises a mounting base, a plurality of separation cylinders are mounted at the upper end of the mounting base through a plurality of telescopic rods, a driving mechanism for driving test tubes to rotate is arranged in each separation cylinder, and each driving mechanism comprises a first motor fixedly connected to the inner bottom of the corresponding separation cylinder; a centrifugal rotor is installed on an output shaft of the first motor, a sealing cover is arranged on the separation cylinder, a plurality of control panels are arranged at the upper end of the installation base, and an air inlet and an air outlet are formed in the side wall of the separation cylinder. According to the utility model, the driving mechanism is arranged, so that centrifugal separation of a plurality of experimental groups can be carried out on the equipment, a large amount of waiting time is saved, the cell separation efficiency is improved, and the practicability of the device is further improved.

Description

Technical Field

[0001] The utility model relates to the technical field of cell separation devices, in particular to a high-efficiency cell separation device. Background Art

[0002] Cell separation techniques include centrifugation, flow cytometry and cell electrophoresis. Centrifugation is a basic means to study the nucleus, mitochondria, Golgi apparatus, lysosomes and microsomes, as well as various macromolecules. Flow cytometry is a technique for rapid quantitative analysis and sorting of single cells.

[0003] In the prior art, the cell separation device is only provided with one centrifugal chamber, and the centrifugation steps are carried out in sequence according to the cell homogenate, chloroplasts, mitochondria, lysosome fluid (plant cells), endoplasmic reticulum, Golgi body, vesicle fluid, and ribosomes, which takes a lot of time. When there are many experimental groups, the separation efficiency of one centrifugal chamber is low. Therefore, it is necessary to design an efficient cell separation device to solve the above problems. Utility Model Content

[0004] The utility model aims to solve the shortcomings in the prior art and proposes a high-efficiency cell separation device.

[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0006] A high-efficiency cell separation device comprises a mounting base, wherein a plurality of separation cylinders are mounted on the upper end of the mounting base via a plurality of telescopic rods, a driving mechanism for driving a test tube to rotate is arranged inside the separation cylinder, the driving mechanism comprises a first motor fixedly connected to the bottom of the separation cylinder, a centrifugal rotor is mounted on the output shaft of the first motor, a sealing cover is arranged on the separation cylinder, a plurality of control panels are arranged on the upper end of the mounting base, an air inlet and an air outlet are arranged on the side wall of the separation cylinder, and the lower end of the separation cylinder is connected to the mounting base via a connecting mechanism.

[0007] Preferably, the connection mechanism comprises a plurality of shock absorbers arranged at the lower end of the separation cylinder, the lower end of the shock absorber is fixedly connected to the upper end of the mounting base, and a spring is provided on the shock absorber.

[0008] Preferably, dustproof nets are provided on the air inlet and the air outlet, and a fan is provided at the bottom of the separation cylinder.

[0009] Preferably, a fixing frame and a limiting rod are fixedly connected to the upper end of the mounting base, a sliding frame is slidably connected to the limiting rod, and a plurality of brush plates are fixedly connected to the side wall of the sliding frame.

[0010] Preferably, a reciprocating screw is rotatably connected to the fixed frame, the lower end of the reciprocating screw passes through the lower end of the sliding frame, and a sliding groove is provided on the side wall of the fixed frame.

[0011] Preferably, a second motor is fixedly connected to the upper end of the mounting base, and an output shaft of the second motor is fixedly connected to the lower end of the reciprocating screw.

[0012] The utility model has the following beneficial effects:

[0013] The device is provided with a driving mechanism, and the four cell separation steps can be distributed in four separation cylinders, and an experimenter can centrifuge other experimental groups or other experimental object cells after using the separation cylinder, which saves a lot of waiting time, thereby improving the cell separation efficiency;

[0014] The device is provided with a connection mechanism, which can buffer the vibration generated by the first motor through the cooperation of the shock absorber and the spring, reduce the impact of the vibration on the equipment, and thus improve the practicality of the device;

[0015] The device is provided with a brush plate and a reciprocating screw, and the sliding frame can be moved up and down by rotating the reciprocating screw to scrape off the dust on the dustproof net. Rotating one reciprocating screw can clean multiple dustproof nets, thereby improving the cleaning efficiency of the dustproof net. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the structure of a highly efficient cell separation device proposed by the utility model;

[0017] Figure 2 This is a side view of a high-efficiency cell separation device proposed by the utility model;

[0018] Figure 3 This is a top view of a high-efficiency cell separation device proposed by the utility model.

[0019] In the figure: 1 separation cylinder, 2 telescopic rod, 3 sealing cover, 4 control panel, 5 fan, 6 air inlet, 7 centrifugal rotor, 8 air outlet, 9 shock absorber, 10 mounting base, 11 sliding frame, 12 reciprocating screw, 13 first motor, 14 spring, 15 limit rod, 16 fixed frame, 17 second motor, 18 brush plate. DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0021] Reference Figure 1-3A high-efficiency cell separation device comprises a mounting base 10, a plurality of separation cylinders 1 are mounted on the upper end of the mounting base 10 through a plurality of telescopic rods 2, a driving mechanism for driving the test tube to rotate is arranged in the separation cylinder 1, the driving mechanism comprises a first motor 13 fixedly connected to the bottom of the separation cylinder 1, the model of the first motor 13 is NFC-621-4, a centrifugal rotor 7 is mounted on the output shaft of the first motor 13, the centrifugal rotor 7 is the centrifugal rotor 7 in the prior art, and the connection method between the centrifugal rotor 7 and the output shaft of the first motor 13 is also the prior art, a sealing cover 3 is arranged on the separation cylinder 1, a plurality of control panels 4 are arranged on the upper end of the mounting base 10, an air inlet 6 and an air outlet 8 are arranged on the side wall of the separation cylinder 1, and the lower end of the separation cylinder 1 is connected to the mounting base 10 through a connecting mechanism.

[0022] The connection mechanism includes a plurality of shock absorbers 9 arranged at the lower end of the separation cylinder 1. The lower end of the shock absorber 9 is fixedly connected to the upper end of the mounting base 10. A spring 14 is arranged on the shock absorber 9. The shock absorber 9 and the spring 14 are damping shock absorbers in the prior art.

[0023] In the utility model, dustproof nets are provided on the air inlet 6 and the air outlet 8 to prevent dust from entering. A fan 5 is provided at the bottom of the separation cylinder 1 to accelerate the air flow in the separation cylinder 1 and improve the heat dissipation efficiency of the first motor 13.

[0024] In the utility model, a fixing frame 16 and a limiting rod 15 are fixedly connected to the upper end of the mounting base 10, a sliding frame 11 is slidably connected to the limiting rod 15, the cross-section of the sliding frame 11 is a cross structure, the middle part of the sliding frame 11 is circular, and a plurality of brush plates 18 are fixedly connected to the side walls of the sliding frame 11. The details of the bristles of the brush plates 18 are not shown in the figure, and the dust screens in the plurality of air inlets 6 can be cleaned simultaneously, thereby improving the cleaning efficiency of the dust screens.

[0025] In the utility model, a reciprocating screw 12 is rotatably connected to the fixed frame 16, and the lower end of the reciprocating screw 12 passes through the lower end of the sliding frame 11. A sliding groove is opened on the side wall of the fixed frame 16, and the sliding groove is used for the movement of the sliding frame 11. The sliding frame 11 can be driven to rise and fall by rotating the reciprocating screw 12, and there is no need for manual lifting back and forth.

[0026] In the utility model, a second motor 17 is fixedly connected to the upper end of the mounting base 10, and the output shaft of the second motor 17 is fixedly connected to the lower end of the reciprocating screw 12. The second motor 17 model is an external drive screw integrated stepper motor SPS86E276-5.08-150, which can rotate the reciprocating screw 12 without manual operation.

[0027] When in use, open the sealing cover 3, put one or more test tubes containing cell homogenates on the centrifuge rotor 7, the test tubes are placed symmetrically, and the missing parts are replaced by clean water test tubes, start the first motor 13, and the output shaft of the first motor 13 drives the test tube to rotate. The cell homogenate rotates to separate the cell homogenate according to the centrifugal speed and time in the prior art, and the cell nuclear fluid is separated. The supernatant is poured into an empty test tube, and then the cell nuclear fluid is continued to be separated in one of the separation cylinders 1. The previous separation cylinder 1 can be used for centrifugation of other experimental groups or other experimental object cell homogenates. After the separation of the second separation cylinder 1 is completed, the supernatant obtains chloroplasts, mitochondria, and lysosome fluid (plant cells), which are then placed in the third separation cylinder 1 for separation, and the supernatant obtains endoplasmic reticulum, Golgi body, and vesicle fluid, and finally placed in the fourth separation cylinder 1 for ribosome separation. During the process, the complete cell extraction process can be carried out in four separation cylinders 1, and the separation cylinder 1 that has been used can be used for centrifugation of other experimental groups or other experimental object cells, which saves a lot of waiting time, thereby improving the cell separation efficiency;

[0028] When the separation cylinder 1 is working, the fan 5 is started to accelerate the external air into the separation cylinder 1 from the air inlet 6, and the hot air is discharged from the air outlet 8, so that the heat dissipation effect of the first motor 13 can be improved.

[0029] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and practical concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A highly efficient cell separation device, comprising a mounting base (10), characterized in that: A plurality of separation barrels (1) are mounted on the upper end of the mounting base (10) via a plurality of telescopic rods (2); a driving mechanism for driving the test tube to rotate is provided in the separation barrel (1); the driving mechanism comprises a first motor (13) fixedly connected to the bottom of the separation barrel (1); a centrifugal rotor (7) is mounted on the output shaft of the first motor (13); a sealing cover (3) is provided on the separation barrel (1); a plurality of control panels (4) are provided on the upper end of the mounting base (10); an air inlet (6) and an air outlet (8) are provided on the side wall of the separation barrel (1); and the lower end of the separation barrel (1) is connected to the mounting base (10) via a connecting mechanism.

2. A highly efficient cell separation device according to claim 1, characterized in that: The connection mechanism comprises a plurality of shock absorbers (9) arranged at the lower end of the separation cylinder (1), the lower end of the shock absorber (9) being fixedly connected to the upper end of the mounting base (10), and a spring (14) being provided on the shock absorber (9).

3. A highly efficient cell separation device according to claim 2, characterized in that: The air inlet (6) and the air outlet (8) are both provided with dustproof nets, and a fan (5) is provided at the bottom of the separation cylinder (1).

4. A highly efficient cell separation device according to claim 3, characterized in that: The upper end of the mounting base (10) is fixedly connected to a fixing frame (16) and a limiting rod (15), the limiting rod (15) is slidably connected to a sliding frame (11), and the side wall of the sliding frame (11) is fixedly connected to a plurality of brush plates (18).

5. A highly efficient cell separation device according to claim 4, characterized in that: A reciprocating screw (12) is rotatably connected to the fixed frame (16), the lower end of the reciprocating screw (12) passes through the lower end of the sliding frame (11), and a sliding groove is formed on the side wall of the fixed frame (16).

6. The high-efficiency cell separation device according to claim 5, characterized in that: A second motor (17) is fixedly connected to the upper end of the mounting base (10), and an output shaft of the second motor (17) is fixedly connected to the lower end of the reciprocating screw (12).

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