Dynamic pressure incubator

Abstract

The utility model aims to provide a dynamic pressure incubator. Wherein the dynamic pressure incubator comprises a box body, provides a culture space and is provided with a pressure interface; the pressure device comprises a driving mechanism and a telescopic mechanism, the distance between the moving end of the telescopic mechanism and the pressure connector provides a variable pressure space, and the variable pressure space is directly communicated with the pressure connector, so that the variable pressure space is directly connected with the culture space; the driving mechanism is connected with the moving end of the telescopic mechanism, and the telescopic mechanism can be driven by the driving mechanism to move so as to change the distance between the moving end and the pressure interface, so that the volume of the variable-pressure space is changed, and the pressure of the culture space is changed.

Description

Technical Field

[0001] The present application relates to a dynamic pressure incubator. Background Art

[0002] An incubator is a box device mainly used for cultivating microorganisms, plant and animal cells. It simulates the growth environment of microorganisms, tissues, cells, etc., provides stable temperature, humidity and gas concentration, and is widely used in the reproduction and cultivation of cells, tissue culture and certain special microorganisms.

[0003] In some prior art solutions, it is proposed to provide a pressurized gas environment with additional pressure added above the atmospheric pressure, and to perform culture, such as stem cell culture, in the pressurized gas environment.

[0004] For example, in a patent document with publication number WO2022012608A1 and titled “Stem Cell Cultivation Method”, a stem cell cultivation method is proposed, comprising the steps of culturing stem cells in a gas environment with additional pressure in addition to atmospheric pressure, wherein the additional pressure is 60 to 140 mmHg (similar to human blood pressure), for example, 70 to 120 mmHg, 75 to 115 mmHg, 80 to 110 mmHg, 85 to 100 mmHg or 90 to 95 mmHg.

[0005] For another example, in the patent document entitled “Incubator” with publication number WO2022012607A1, the incubator includes a housing and a transformer, wherein the housing has a transformer interface, and the transformer is connected to the housing through the transformer interface, and the transformer is used to input fluctuating or constant air pressure into the housing. The transformer may include a cylinder and a piston. However, the inventors found that if a larger pressure needs to be provided, the large-diameter sealing ring required for the piston leads to large friction resistance and low efficiency of the system. In addition, the frictional heat generated by the piston and the cylinder caused by the reciprocating motion of the piston will affect the stability of the temperature in the housing, and the volatilization of the grease or lubricating oil required by the piston will affect the gas environment of the culture. Therefore, for situations with higher pressure, the structure of the piston drive needs to be improved.

[0006] In addition, in some comparative schemes, the pressure of the pressure device is transmitted to the incubator through a pipeline. However, the inventors found that this scheme is also difficult to achieve a higher culture pressure to meet the culture requirements. For example, it is impossible to achieve a pressure environment that simulates human blood pressure (human blood pressure is 60 to 140 mmHg, converted to kPa, that is, the normal systolic pressure is 12 kPa to 18.6 kPa, and the normal diastolic pressure is 8 kPa to 12 kPa). Utility Model Content

[0007] The utility model aims to realize that on the basis of satisfying the culture pressure, the temperature stability of the culture box and the gas environment meet the culture requirements.

[0008] According to the first aspect of the present application, a dynamic pressure incubator comprises a box body, which provides a culture space and has a pressure interface; a pressure device comprises a driving mechanism and a telescopic mechanism, wherein the distance between the moving end of the telescopic mechanism and the pressure interface provides a variable pressure space, and the variable pressure space is directly connected to the pressure interface so that the variable pressure space is directly connected to the culture space; the driving mechanism is connected to the moving end of the telescopic mechanism, and the telescopic mechanism can be driven by the driving mechanism to move, so as to change the distance between the moving end and the pressure interface, thereby changing the volume of the variable pressure space, thereby changing the pressure of the culture space.

[0009] In one or more embodiments of the dynamic pressure incubator, the fixed end of the telescopic mechanism is fixed to the pressure interface, so that the internal space of the telescopic mechanism from the fixed end to the movable end provides the variable pressure space.

[0010] In one or more embodiments of the dynamic pressure incubator, the pressure device also includes an outer shell, which is a rigid shell, one end of which is fixedly connected to the pressure interface, and the other end of which is connected to the fixed end of the telescopic mechanism, and the telescopic mechanism is arranged inside the outer shell.

[0011] In one or more embodiments of the dynamic pressure incubator, the telescopic mechanism comprises a telescopic sleeve, and the shape of the telescopic sleeve is selected from corrugated, spherical, and gourd-shaped; the material of the telescopic sleeve is selected from elastomeric materials, plastics, and metals.

[0012] In one or more embodiments of the dynamic pressure incubator, the telescopic sleeve of the telescopic mechanism includes a telescopic sleeve body and a reinforcement ring.

[0013] In one or more embodiments of the dynamic pressure incubator, the driving mechanism is selected from an electric cylinder, a pneumatic cylinder, a hydraulic cylinder, or a crankshaft-connecting rod mechanism; the output end of the driving mechanism is connected to the moving end of the telescopic mechanism; preferably, the electric cylinder includes a servo motor and a transmission mechanism, and the transmission mechanism connects the servo motor and the moving end of the telescopic mechanism to convert the rotation of the servo motor into the movement of the moving end of the telescopic mechanism.

[0014] In one or more embodiments of the dynamic pressure incubator, the pressure device is fixedly connected to the box body; preferably, the pressure device is fixedly connected to the top of the box body; when the fixed end of the telescopic mechanism is fixed to the pressure interface, the lower end of the telescopic sleeve is the fixed end, which is fixedly connected to the box body; when the pressure device includes an outer shell, the lower end of the outer shell is fixedly connected to the box body, the upper end of the outer shell is connected to the upper end of the telescopic mechanism, and the driving mechanism is connected to the lower end of the telescopic mechanism, so that the distance between the telescopic mechanism and the pressure interface provides the pressure variable space.

[0015] In one or more embodiments of the dynamic pressure incubator, the box body also includes a temperature sensor, a humidity sensor, a carbon dioxide gas concentration sensor, an oxygen concentration sensor and / or a gas pressure sensor, which is used to be electrically connected to the control unit; preferably, the box body also includes an openable and closable air inlet and an openable and closable air outlet independent of the pressure interface, which are used to input or output one or more gases of the dynamic pressure incubator; more preferably, the gas includes carbon dioxide, nitrogen and / or oxygen.

[0016] In one or more embodiments of the dynamic pressure incubator, the dynamic pressure incubator includes a multi-layer structure, including at least a first layer and a second layer, the first layer defines the culture space, and the second layer is located outside the first layer.

[0017] In one or more embodiments of the dynamic pressure incubator, the pressure device provides a periodic fluctuation of the pressure of the box body with a maximum value of 30 kPa; preferably, the pressure of the box body fluctuates periodically between 8 kPa and 20 kPa; more preferably, the pressure of the box body fluctuates periodically between 10 kPa and 15 kPa.

[0018] According to a second aspect of the present application, a culture method using the dynamic pressure culture box as described in the first aspect comprises the following steps:

[0019] S1. Placing the culture object in the culture space of the box;

[0020] S2. The culture space is closed, and the temperature, humidity, carbon dioxide concentration, oxygen concentration and pressure in the culture space reach the set values;

[0021] S3. Start the pressure device, the movable end of the telescopic mechanism is driven by the driving mechanism to move, and the size of the variable pressure space is changed to provide periodic pressure fluctuations to the culture space, wherein the maximum value of the periodic pressure is 30 kPa, preferably, the periodic pressure fluctuation is 8 kPa-20 kPa, and more preferably, the periodic pressure fluctuation is 10 kPa to 15 kPa;

[0022] S4. After the culture is completed, the pressure in the culture space is released and the cultured object can be taken out.

[0023] In one or more embodiments of the dynamic pressure incubator, it further includes: a memory for storing instructions executable by a processor; a processor for executing the instructions to implement the steps of the culture method of the second aspect that can be implemented by a computer program.

[0024] According to a third aspect of the present application, a computer-readable storage medium has a computer program thereon, and the program is executed by a processor to implement the steps in the culture method described in the second aspect that can be implemented by the computer program. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] The above and other features, properties and advantages of the present invention will become more apparent through the following description in conjunction with the accompanying drawings and embodiments, in which:

[0026] Figure 1 It is a schematic diagram of the external structure of the dynamic pressure incubator of the first embodiment.

[0027] Figure 2 It is a schematic diagram of the internal structure of the dynamic pressure incubator of the first embodiment.

[0028] Figure 3 It is a schematic diagram of the external structure of the dynamic pressure incubator of the second embodiment.

[0029] Figure 4 It is a schematic diagram of the internal structure of the dynamic pressure incubator of the second embodiment.

[0030] Figure 5 It is a schematic block diagram of the structure of a dynamic pressure incubator according to an embodiment.

[0031] Figure 6 It is a schematic diagram of a culture method according to an embodiment. DETAILED DESCRIPTION

[0032] The following discloses a variety of different implementation methods or embodiments of the subject technical solution. To simplify the disclosure, specific examples of each element and arrangement are described below. Of course, these are only examples and are not intended to limit the scope of protection of the present application. For example, the first feature subsequently recorded in the specification is formed above or on the second feature, which may include an implementation method in which the first and second features are formed by direct connection, and may also include an implementation method in which an additional feature is formed between the first and second features, so that the first and second features may not be directly connected. In addition, the figure marks and / or letters may be repeated in different examples in these disclosures. This repetition is for simplicity and clarity, and does not itself represent the relationship between the various implementation methods and / or structures to be discussed. Further, when the first element is described in a manner connected or combined with the second element, the description includes an implementation method in which the first and second elements are directly connected or combined with each other, and also includes the use of one or more other intervening elements to indirectly connect or combine the first and second elements.

[0033] It is to be understood that the following uses a flow chart to illustrate the steps performed by the culture method according to the embodiments of the present application. It should be understood that, according to actual circumstances, the preceding or following steps may not necessarily be performed accurately in order. Other steps may also be added to these processes, or a step or several steps may be removed from these processes.

[0034] refer to Figures 1 to 4 As shown, the dynamic pressure incubator 100 includes a pressure device 1 and a box 2. The dynamic pressure incubator 100 is applicable to culture objects such as standard T25 cell culture flasks, T75 cell culture flasks, T300 cell culture flasks, cell factories, multi-well culture plates, etc. The internal cavity generally needs to be sterilized before use, and the sterilization methods include ultraviolet irradiation sterilization, 140° dry heat sterilization, ethylene oxide sterilization, hydrogen peroxide sterilization, etc.

[0035] The housing 2 provides a culture space 21 for cell culture and a pressure interface 22 directly connected to the pressure device 1 . The pressure interface 22 here means receiving the fluctuating pressure or constant pressure output by the pressure device to the culture space 21 .

[0036] Preferably, reference Figures 1 to 4As shown, the specific structure of the box 2 can be a cube / cuboid structure formed by welding multiple plates, and the material can be stainless steel. An air duct can be arranged inside the box 2, and the air duct is used to promote the airflow circulation in the culture space 21 to improve the gas uniformity in the culture space 21. A fan and a high-efficiency filter can also be arranged in the air duct, and the high-efficiency filter can filter and clean the gas. Preferably, the box 2 includes a multi-layer structure, for example, at least a first layer and a second layer, the first layer defines the culture space 21, and the second layer is located at the periphery of the first layer. For example, a two-layer structure, the inner layer defines the culture space 21, and the opening and closing of the inner layer door can be controlled by a solenoid valve, and the outer layer serves as insulation and protection. After the box 2 is closed, the outer door of the outer layer and the inner door of the inner layer need to be opened in sequence before the culture object in the culture space 21 can be placed or taken out. The multi-layer structure can be sealed by an inflatable seal, for example, the inner door of the inner layer and / or the outer door of the outer layer are sealed by an inflatable seal to the culture space 21.

[0037] refer to Figure 5 As shown, in some embodiments, the housing 2 may also include a temperature sensor 23, a humidity sensor 24, a carbon dioxide gas concentration sensor 25, an oxygen concentration sensor 26, and a gas pressure sensor 27, which are used to be electrically connected to the control unit. It can be understood that the above sensor description is only an introduction to the function, which can be a distributed setting, or multiple functions can be integrated into a single sensor. Correspondingly, the housing 2 may also include an openable and closable air inlet and an openable and closable air outlet independent of the pressure interface 22, which are used to input or output one or more gases in the dynamic pressure incubator, for example, the gas includes carbon dioxide, nitrogen, and oxygen, so as to keep the gas environment in the culture space 21 during the culture process to meet the requirements, for example, to meet the temperature of 37°, the carbon dioxide concentration of 5%, and the oxygen concentration of 3%. The specific adjustment method may be that the data sensed and collected by the carbon dioxide gas concentration sensor 25 and the oxygen concentration sensor 26 are transmitted to the processor 31 of the control unit 3, and the processor 31 judges and outputs a control signal to the gas source according to the sensed data to instruct the delivery of carbon dioxide or nitrogen or oxygen, so that the gas environment meets the requirements. Similarly, the data sensed by the temperature sensor 23, the humidity sensor 24, and the gas pressure sensor 27 are transmitted to the processor 31 of the control unit 3. The processor 31 judges and outputs control signals to the temperature change device (such as a heater), the humidity source (such as a humidification tank), and the pressure source (i.e., the pressure device 1) based on the sensed data, so that the temperature, humidity, and pressure meet the culture requirements.

[0038] refer to Figures 1 to 4As shown, the structure of the pressure device 1 includes a driving mechanism 12 and a telescopic mechanism 13. The distance between the moving end 131 of the telescopic mechanism 13 and the pressure interface 22 provides a variable pressure space 14, and the variable pressure space 14 is directly connected to the pressure interface 22, so that the variable pressure space 14 is directly connected to the culture space 21. The direct connection and direct connection here refer to the direct connection between the two, excluding the structure that is indirectly connected through intermediate components such as pipes. The driving mechanism 12 is connected to the moving end 131 of the telescopic mechanism 13, and the telescopic mechanism 13 can be driven by the driving mechanism 12 to move, so as to change the distance between the moving end 131 and the pressure interface 22, thereby changing the volume of the variable pressure space 14, thereby changing the pressure of the culture space 21.

[0039] The distance between the moving end 131 of the telescopic mechanism 13 and the pressure interface 22 provides a structure of the variable pressure space 14. For example, the telescopic mechanism 13 itself directly provides the variable pressure space 14 ( Figure 1 , Figure 2 The first embodiment shown in the figure) can also form a variable pressure space 14 ( Figure 3 , Figure 4 The specific content will be introduced later.

[0040] The above embodiments have the beneficial effect of ensuring that the temperature stability and gas environment of the incubator meet the requirements of the culture on the basis of meeting the culture pressure. Specifically, the variable pressure space 14 is directly connected to the pressure interface 22, and the telescopic mechanism 13 can be driven by the driving mechanism 12 to move and change the volume of the variable pressure space 14 directly connected to the pressure interface 22. First of all, the telescopic mechanism 13 changes the volume of the directly connected variable pressure space 14, that is, the size of the space for providing pressure, through its own telescopic mechanism, rather than relying on the movement of the piston in the cylinder to change the volume and provide pressure as in the patent WO2022012607A1 introduced in the background technology. Therefore, there is no need to set grease or lubricating oil, which avoids the influence of the volatilization of grease or lubricating oil on the gas environment in the incubator, and also avoids the friction heat between the piston and the cylinder to affect the stability and uniformity of the temperature in the incubator. In addition, the inventors found that the above structure can also provide stable and reliable high pressure, with the maximum pressure reaching 30kPa. It can also simulate the pressure environment changes of mammalian blood pressure. For example, the normal blood pressure of the human body fluctuates between 120 / 80mmHg (16 / 10.66kPa), and the normal blood pressure of a giraffe is 220 / 180mmHg (30 / 24kPa), which makes the incubator have a wide range of applications.

[0041] refer to Figure 1 to Figure 2As shown, in the first embodiment of the dynamic pressure incubator 100, the telescopic sleeve 130 of the telescopic mechanism 13 itself provides a variable pressure space 14, the fixed end 132 of the telescopic mechanism 13 is fixed to the pressure interface 22 (that is, fixed to the box body 2), and the movable end 131 is connected to the driving mechanism 12, so that the internal space 133 of the telescopic mechanism 13 from the fixed end 132 to the movable end 131 provides a variable pressure space 14, and the driving mechanism 12 can drive the movable end 131 of the telescopic mechanism 13 to be closer to or farther away from the fixed end 132 to change the volume of the variable pressure space 14. In the first embodiment, the internal space 133 of the telescopic mechanism 13 itself is the space directly connected to the pressure device 1 and the pressure interface 22. According to the principle of the ideal gas state equation, since the internal space 133 is directly connected to the culture space 21 through the pressure interface 22, when the volume of the internal space 133 decreases, it is equivalent to that the total volume of the culture space 21 and the internal space 133 decreases, that is, the pressure in the culture space 21 and the internal space 133 increases. When the volume of the internal space 133 increases, it is equivalent to that the total volume of the culture space 21 and the internal space 133 increases, and the pressure inside it decreases. Therefore, by driving the telescopic mechanism 13 to be extended and retracted by the driving mechanism 12, it is possible to provide a fluctuating pressure to the culture space 21. The form of the fluctuating pressure can be flexibly adjusted according to actual culture needs, including but not limited to periodic fluctuations of square waves, sawtooth waves, sine waves or quasi-sinusoidal changes. It can be understood that if the driving mechanism 12 drives the telescopic mechanism 13 to move to a fixed position, it is possible to provide a constant pressure to the culture space 21. Figure 1 as well as Figure 2 As shown in , the pressure device 1 can be fixedly connected to the box body 2; preferably, the pressure device 1 is fixedly connected to the top of the box body 2. In the first embodiment, the upper end of the telescopic sleeve 130 is the movable end 131, which is fixedly connected to the driving mechanism 12, and the lower end is the fixed end 132, which is fixedly connected to the box body 2 through the connecting flange 134 and the seal, so that the variable pressure space 14 (i.e., the internal space 133) is directly connected to the pressure interface 22. The inside and outside of the telescopic mechanism 13 here mean that the inside refers to the part within the scope of the telescopic mechanism 13 itself, such as the part inside the telescopic sleeve 130, and the outside refers to the part outside the telescopic sleeve 130.

[0042] refer to Figure 3 to Figure 4As shown, in the second embodiment of the dynamic pressure incubator 100, it also includes a shell 11, which is a non-retractable rigid shell, one end 111 of the shell 11 is fixedly connected to the pressure interface 22, and the other end 112 is connected to the fixed end 132 of the telescopic mechanism 13, and the telescopic mechanism 13 is arranged inside the space defined by the shell 11. In this way, a structure is formed in which the telescopic mechanism 13 and the shell 11 jointly define the variable pressure space 14. Specifically, the driving mechanism 12 can extend into the interior of the telescopic mechanism 13 and connect with the moving end 131 of the telescopic mechanism 13, so that the distance 135 between the moving end 131 of the telescopic mechanism 13 and one end of the shell 11 located outside the telescopic mechanism 13 (i.e., the pressure interface 22) provides a variable pressure space 14, and the driving mechanism 12 can drive the mobile end 131 to move closer to or farther away from the pressure interface 22 located outside the mobile end 131, so as to change the volume of the variable pressure space 14. The principle is that, according to the principle of the ideal gas state equation, since the radial gap between the telescopic mechanism 13 and the inner wall of the housing 11 can be regarded as constant, the two do not need to be in contact, and the output pressure is determined by the distance between the moving end 131 of the telescopic mechanism 13 and one end 111 of the housing 11. When the driving mechanism 12 drives the moving end 131 of the telescopic mechanism 13 to approach the one end 111 of the housing 11, the distance 135 decreases, and the variable pressure space 14 decreases, that is, the provided pressure increases. When the driving mechanism 12 drives the telescopic mechanism 13 to move away from the above-mentioned one end, the distance 135 increases, that is, the variable pressure space 14 increases, that is, the provided pressure decreases. Figure 3 as well as Figure 4 As shown, the lower end of the housing 1 is fixedly connected to the pressure interface 22 of the box body 2 through a connecting flange and a seal, and the upper end is connected to the upper end of the telescopic mechanism 13. The driving mechanism 12 can extend into the telescopic mechanism 13 and connect to the lower end (i.e., the moving end 131) of the telescopic mechanism 13. When the lower end of the telescopic mechanism 13 moves close to the pressure interface 22, the output pressure is large, and when the lower end of the telescopic mechanism 13 moves away from the pressure interface 22, the output pressure is small.

[0043] It can be understood that, compared with the first embodiment and the second embodiment, the pressure regulation efficiency of the dynamic pressure incubator of the second embodiment is higher. The principle is that, since the driving mechanism 12 of the second embodiment extends into the interior of the telescopic mechanism 13 to drive its movable end 132, the driving stroke of the driving mechanism 12 is accommodated by the space of the telescopic mechanism 13, so the volume change ratio of the second embodiment is larger. The telescopic mechanism is arranged in the shell, which can reduce the heat exchange between the telescopic mechanism and the outside and improve the temperature stability of the culture space 21 inside the incubator. In the first embodiment, the driving mechanism 12 provides a driving stroke on the outside, that is, the dimension in the height direction is the driving stroke of the driving rod mechanism plus the dimension of the telescopic mechanism. Therefore, the structure of the second embodiment is more compact. However, since the telescopic sleeve 130 of the telescopic mechanism 13 has its own variable pressure space 14, the first embodiment has fewer components and does not require an additional shell, so the cost is lower.

[0044] Preferably, reference Figure 2 as well as Figure 4 As shown, in some embodiments, the shape of the telescopic sleeve of the telescopic mechanism 13 includes a corrugated telescopic sleeve. The beneficial effect of this is that the pressure change output can be achieved through the telescopic amount of each wave node, which is easy to control, but it is not limited to this. For example, it can also be a ball-shaped or gourd-shaped shape. The material of the telescopic sleeve can be selected from elastomeric materials, plastics, metals, etc.; the elastomeric materials include thermosetting elastomers (such as rubber, silicone, etc.) and thermoplastic elastomers. Preferably, the telescopic sleeve 130 includes a telescopic sleeve body 1301 and a reinforcement ring 1302. For example, for a corrugated telescopic sleeve, the reinforcement ring 1302 can be set at the position of the wave node, and the service life and reliability of the telescopic mechanism 13 are further improved.

[0045] refer to Figures 1 to 4 As shown, in some embodiments, the specific structure of the driving mechanism 12 can be one of an electric cylinder, a pneumatic cylinder, a hydraulic cylinder, or a crankshaft connecting rod mechanism; the output end of the driving mechanism 12 is connected to the moving end of the telescopic mechanism 13 to drive the telescopic mechanism 13 to move and output a predetermined pressure. Taking the electric cylinder shown in the figure as an example, the electric cylinder can include a servo motor 121 and a transmission mechanism 122, and the transmission mechanism 122 connects the servo motor 121 and the moving end 131 of the telescopic mechanism 13 to convert the rotation of the servo motor 121 into the movement of the moving end 131 of the telescopic mechanism 13; for example, the electric cylinder is an electric push rod or a parallel electric cylinder, and the electric push rod can directly drive the moving end 131 of the telescopic mechanism 13 to move, but it is not limited to this.

[0046] refer to Figure 5As shown, the pressure output by the pressure device 1 can be controlled by a control unit 3. The control unit 3 generally includes a processor 31 and a memory 32. The memory 32 is used to store instructions executable by the processor 31. The processor 31 is used to execute the instructions to achieve, for example, the above-mentioned control of the driving mechanism 12 of the pressure device 1 to control the output pressure, and control the temperature, humidity, carbon dioxide concentration, oxygen concentration and other parameters in the culture space 21 to meet the culture requirements. It should be noted that Figure 5 It is only to clearly illustrate the control relationship, and does not mean that the pressure device 1 and the box 2 are indirectly connected through a pipeline. As mentioned above, the two are directly connected, that is, the movement of the telescopic device 13 causes the space with a changed size to be directly connected to the culture space 21 of the box 2 to output pressure to the culture space 21.

[0047] like Figure 6 As shown, as described above, the present application further provides a culture method, using the culture device 100 described in the above embodiment, the culture method comprises the following steps:

[0048] S1. The culture object is placed in the culture space 21 of the box 2;

[0049] For example, the outer door and the inner door of the box 2 can be opened in sequence, and the culture vessels can be placed in the box 2. Generally, it is necessary to place them in a dispersed manner to prevent stacking from obstructing the flow of gas in the box 2. For the inner door with an electromagnetic lock, the specific operation method can be to press and hold the electromagnetic lock switch of the inner door, retract the electromagnetic lock core on the inner door, push the inner door to a position parallel to the door frame, release the electromagnetic lock core and pop it out, and the internal sensor detects the position of the lock core and the system determines whether it is closed, and then close the outer door (i.e., the insulation door).

[0050] S2. The culture space 21 is closed, and the temperature, humidity, carbon dioxide concentration, oxygen concentration and pressure in the culture space 21 reach the set values.

[0051] For example, set the temperature, carbon dioxide concentration, oxygen concentration, minimum dynamic pressure value, maximum dynamic pressure value, etc., and detect that the set value is within the allowable range of the equipment. For example, the data is set as follows: temperature 37°, carbon dioxide concentration 5%, oxygen concentration 3%. For example, if you need to simulate the environment of human blood pressure, the minimum dynamic pressure value is 10kPa and the maximum dynamic pressure value is 15kPa. For example, you can click the start button, and the control unit 3 of the incubator 100 will automatically complete the self-detection, and the electric cylinder will return to zero. The control unit 3 automatically completes the gas concentration configuration in the box 2 through the inflation and deflation program, and stops inflation after reaching the set value.

[0052] S3. Start the pressure device 1, the moving end 131 of the telescopic mechanism 13 is driven by the driving mechanism 12 to move, and the size of the variable pressure space 14 is changed to provide periodic pressure fluctuations to the culture space. As described above, the maximum value of the periodic pressure fluctuation in the culture space 21 is 30 kPa, preferably, the periodic pressure fluctuation is 8 kPa-20 kPa, and more preferably, the periodic pressure fluctuation is 10 kPa to 15 kPa;

[0053] For example, the electric cylinder starts to operate, and the driving mechanism 12 compresses the rubber telescopic sleeve 130 downward from the highest position. The air in the rubber telescopic sleeve 130 is squeezed into the box 2, causing the pressure inside the box 2 to increase, and vice versa. The pressure in the culture vessel changes with the pressure inside the box 2, and then is transmitted to the biological tissue in the culture vessel. The biological tissue senses the change in gas pressure, which affects its life activities.

[0054] S4. After the culture is completed, the pressure in the culture space 21 is released and the cultured object can be taken out.

[0055] Specifically, at the end of the operation, the control unit 3 stops the operation of the electric cylinder and stops the gas supply, releases the pressure in the box 2 through the deflation procedure, and releases the pressure on the inflatable sealing ring of the inner door to release the seal. The culture personnel presses the inner door electromagnetic lock switch to open the inner door and take out the culture vessel.

[0056] It can be understood that, during the culture process before S4, the box 2 remains sealed, and parameters such as the temperature, humidity, carbon dioxide gas concentration, oxygen concentration and gas pressure variation range inside the box 2 can be set by the user.

[0057] According to another aspect of the application, the present application also provides a computer-readable medium.

[0058] The computer-readable medium provided in the present application has computer instructions thereon. When the computer instructions are executed by a processor, the program can be executed by the processor to implement the steps executed by the program in the culture method described in the above embodiment.

[0059] It should be noted that the above-mentioned memory, processor, and database are not limited to a specific memory, processor, and database. For example, in some cases, the memory and processor can have a distributed structure, for example, the memory and processor can be respectively located at the incubator end and the background cloud, and the incubator end and the background cloud jointly implement the above-mentioned culture method. Furthermore, in the embodiment adopting a distributed structure, each step can adjust the specific execution terminal according to the actual situation, and the specific scheme of each step implemented in a specific terminal should not limit the scope of protection of this application.

[0060] Another aspect of the present application further provides a computer-readable storage medium storing a computer program. When the computer program is executed by a processor, the steps of the cultivation method described in the above embodiment are implemented. Please refer to the above description for details, which will not be repeated here.

[0061] In addition, it can be understood that the above-mentioned computer-readable storage medium can also be in a system form, that is, it includes multiple computer-readable storage sub-media, so as to jointly implement the steps of the culture method described above through multiple computer-readable storage media.

[0062] The steps of the method described in conjunction with the embodiments disclosed herein may be embodied directly in hardware, in a software module executed by a processor, or in a combination of the two. The software module may reside in a RAM memory, a flash memory, a ROM memory, an EPROM memory, an EEPROM memory, a register, a hard disk, a removable disk, a CD-ROM, or any other form of storage medium known in the art. An exemplary storage medium is coupled to a processor so that the processor can read and write information from / to the storage medium. In an alternative, a storage medium may be integrated into a processor. The processor and the storage medium may reside in an ASIC. The ASIC may reside in a user terminal. In an alternative, the processor and the storage medium may reside in a user terminal as discrete components.

[0063] In one or more exemplary embodiments, the functions described may be implemented in hardware, software, firmware, or any combination thereof. If implemented as a computer program product in software, each function may be stored on or transmitted by a computer-readable medium as one or more instructions or codes. Computer-readable media include both computer storage media and communication media, including any medium that facilitates the transfer of a computer program from one place to another. Storage media may be any available medium that can be accessed by a computer. As an example and not limitation, such a computer-readable medium may include RAM, ROM, EEPROM, CD-ROM or other optical disk storage, disk storage or other magnetic storage device, or any other medium that can be used to carry or store the desired program code in the form of an instruction or data structure and can be accessed by a computer. Any connection is also properly referred to as a computer-readable medium. For example, if the software is transmitted from a website, server, or other remote source using a coaxial cable, fiber optic cable, twisted pair, digital subscriber line (DSL), or wireless technologies such as infrared, radio, and microwaves, the coaxial cable, fiber optic cable, twisted pair, DSL, or wireless technologies such as infrared, radio, and microwaves are included in the definition of the medium. Disk and disc as used herein include compact disc (CD), laser disc, optical disc, digital versatile disc (DVD), floppy disk and Blu-ray disc, wherein disk often reproduces data magnetically, while disc reproduces data optically with lasers. Combinations of the above should also be included within the scope of computer-readable media.

[0064] Although the utility model is disclosed as above with preferred embodiments, it is not intended to limit the utility model. Any person skilled in the art can make possible changes and modifications without departing from the spirit and scope of the utility model. Therefore, any modification, equivalent change and modification made to the above embodiments based on the technical essence of the utility model without departing from the content of the technical solution of the utility model shall fall within the protection scope defined by the claims of the utility model.

Claims

1. Dynamic pressure incubator, characterized in that: include: The box body provides a culture space and has a pressure interface; The pressure device comprises a driving mechanism and a telescopic mechanism, wherein the distance between the moving end of the telescopic mechanism and the pressure interface provides a variable pressure space, and the variable pressure space is directly connected to the pressure interface, so that the variable pressure space is directly connected to the culture space; the driving mechanism is connected to the moving end of the telescopic mechanism, and the telescopic mechanism can be driven by the driving mechanism to move, so as to change the distance between the moving end and the pressure interface, thereby changing the volume of the variable pressure space, so that the telescopic mechanism changes the volume of the directly connected variable pressure space through its own telescopic movement, thereby changing the pressure of the culture space.

2. The dynamic pressure incubator according to claim 1, characterized in that: The fixed end of the telescopic mechanism is fixed to the pressure interface, so that the internal space of the telescopic mechanism from the fixed end to the movable end provides the variable pressure space.

3. The dynamic pressure incubator according to claim 1, characterized in that: The pressure device also includes a shell, which is a rigid shell, one end of which is fixedly connected to the pressure interface, and the other end of which is connected to the fixed end of the telescopic mechanism, and the telescopic mechanism is arranged inside the shell.

4. The dynamic pressure incubator according to any one of claims 1 to 3, characterized in that: The telescopic mechanism comprises a telescopic sleeve, the shape of which is selected from a corrugated shape, a spherical shape, and a gourd shape; the material of the telescopic sleeve is selected from an elastomeric material, plastic, and metal.

5. The dynamic pressure incubator according to claim 4, characterized in that: The telescopic sleeve of the telescopic mechanism comprises a telescopic sleeve body and a reinforcement ring.

6. The dynamic pressure incubator according to claim 4, characterized in that: The pressure device is fixedly connected to the top of the box body; when the fixed end of the telescopic mechanism is fixed to the pressure interface, the lower end of the telescopic sleeve is the fixed end, which is fixedly connected to the box body; when the pressure device includes an outer shell, the lower end of the outer shell is fixedly connected to the box body, the upper end of the outer shell is connected to the upper end of the telescopic mechanism, and the driving mechanism is connected to the lower end of the telescopic mechanism, so that the distance between the telescopic mechanism and the pressure interface provides the pressure variable space.

7. The dynamic pressure incubator according to any one of claims 1 to 3, characterized in that: The driving mechanism is selected from an electric cylinder, a pneumatic cylinder, a hydraulic cylinder, or a crankshaft connecting rod mechanism; the output end of the driving mechanism is connected to the moving end of the telescopic mechanism.

8. The dynamic pressure incubator according to claim 7, characterized in that: The electric cylinder includes a servo motor and a transmission mechanism, wherein the transmission mechanism connects the servo motor and the moving end of the telescopic mechanism to convert the rotation of the servo motor into the movement of the moving end of the telescopic mechanism.

9. The dynamic pressure incubator according to any one of claims 1 to 3, characterized in that: The pressure device is fixedly connected to the box body.

10. The dynamic pressure incubator according to any one of claims 1 to 3, characterized in that: The box body also includes a temperature sensor, a humidity sensor, a carbon dioxide gas concentration sensor, an oxygen concentration sensor and / or a gas pressure sensor, which are electrically connected to the control unit.

11. The dynamic pressure incubator according to claim 10, characterized in that: The box body also includes an openable and closable air inlet and an openable and closable air outlet independent of the pressure interface, which are used for the dynamic pressure incubator to input or output one or more gases.

12. The dynamic pressure incubator according to claim 11, characterized in that: The gas includes carbon dioxide, nitrogen and / or oxygen.

13. The dynamic pressure incubator according to any one of claims 1 to 3, characterized in that: The dynamic pressure culture box includes a multi-layer structure, including at least a first layer and a second layer, the first layer defines the culture space, and the second layer is located outside the first layer.

14. The dynamic pressure incubator according to any one of claims 1 to 3, characterized in that: The pressure device provides a periodic fluctuation of the pressure of the box with a maximum value of 30 kPa.

15. The dynamic pressure incubator according to claim 14, characterized in that: The pressure of the box fluctuates periodically between 8 kPa and 20 kPa.

16. The dynamic pressure incubator according to claim 15, characterized in that: The pressure of the box fluctuates periodically between 10 kPa and 15 kPa.

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