Device convenient for sampling plasma in linezolid blood sample

Abstract

The utility model belongs to the technical field of linezolid in-vivo pharmacokinetics, and particularly relates to a device convenient for sampling plasma in a linezolid blood sample, which comprises a containing tube for containing sample blood, an upper cover, a rubber head liquid suction bag and a liquid suction tube, an opening in the upper end of the accommodating pipe is in threaded connection and fixed with the upper cover; a liquid through hole is formed in the upper cover, and the rubber head liquid suction bag is communicated with the liquid through hole; and the pipette is fixed with the inner side end of the upper cover and is communicated with the liquid through hole. The device can realize one-time sampling of 1ml, and meanwhile, the sampling structure is designed on the upper cover, so that the blood sample is prevented from being polluted by an external pollution source.

Description

Technical Field

[0001] The utility model belongs to the technical field of in vivo pharmacokinetics of linezolid, and particularly relates to a device for facilitating plasma sampling in a linezolid blood sample. Background Art

[0002] The pharmacokinetic study of linezolid tablets was conducted as follows: 12 rabbits weighing 1.9-2.7 kg were selected and randomly divided into 2 groups, 6 in each group. The rabbits were fasted for 12 h before administration and had free access to water. 3D printed linezolid tablets and commercially available linezolid tablets were orally administered at a dose of 15 mg kg-1. 0.5 mL of blood was collected from the ear vein at 0.0, 0.5, 1, 1.5, 2, 2.5, 3, 4, 6, 8 and 12 h after administration. The collected blood samples were placed in heparin anticoagulation tubes, centrifuged at 8000 r min-1 for 5 min, plasma was separated, and stored in a -40 ℃ refrigerator for testing. Finally, UPLC-MS / MS was used to determine the concentration of linezolid in plasma, and the pharmacokinetic parameters were calculated by DAS3.0 pharmacokinetic software using a non-compartmental model. The fitted pharmacokinetic parameters were statistically analyzed using Graphpad 6 software.

[0003] In the above determination process, the blood sample is placed in a heparin anticoagulation tube and centrifuged, and the plasma sample is located in the upper layer of the heparin anticoagulation tube. During the detection and analysis process, the technician needs to sample 1 ml of the plasma sample for analysis. However, when the staff uses an external syringe or pipette for sampling, firstly, there is the problem that the external pipette may be contaminated, and secondly, sampling 1 ml with a syringe requires the staff to observe at all times. Summary of the invention

[0004] The utility model aims to provide a device for facilitating plasma sampling in a linezolid blood sample, which can achieve a single sampling of 1 ml. At the same time, the sampling structure is designed on the upper cover to prevent external pollution sources from causing contamination to the blood sample.

[0005] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0006] The device is convenient for sampling plasma in a linezolid blood sample, comprising a containing tube for containing sample blood, an upper cover, a rubber-tipped liquid suction bag and a liquid suction tube; the upper end opening of the containing tube is screwed and fixed to the upper cover; the upper cover is provided with a liquid through hole, and the rubber-tipped liquid suction bag is connected to the liquid through hole; the liquid suction tube is fixed to the inner side end of the upper cover and is connected to the liquid through hole.

[0007] When the blood sample is added with anticoagulant and placed in a holding tube, after centrifugation, the plasma will be located in the upper layer of the holding tube. The length of the pipette is pre-set, and after centrifugation, the pipette is located in the plasma layer. After centrifugation is completed, the plasma is sucked into the pipette by pressing the rubber-tipped suction bag, and the entire tube is filled with exactly 1ml (such as the rubber-tipped dropper in a chemical laboratory). After the plasma is sucked into the pipette, the technician can load the sample into the injection bottle for analysis on the machine.

[0008] Preferably, a sealing sticker is provided at one end of the pipette facing away from the rubber head pipette bag.

[0009] The purpose of the seal is to prevent blood from flowing into the pipette during the centrifugation process, which may contaminate the sample later, and to prevent foreign matter from falling into the pipette.

[0010] Preferably, the device also includes a positioning bracket, which includes a positioning rod, a positioning plate and a positioning ring; one end of the positioning rod is obliquely fixed to the inner wall of the containing tube, and the other end is fixed to the positioning plate; the positioning ring is fixed above the positioning plate, and the end of the pipette tube facing away from the rubber head pipette bag is inserted into the positioning ring.

[0011] The positioning disc is located in the middle of the receiving tube. When the technician rotates the upper cover to the open end of the receiving tube and rotates downward, the pipette will extend into the positioning ring. Since there is a gap between the pipette and the positioning ring, the pipette can rotate after being inserted into the positioning ring. The positioning ring also plays a positioning role to prevent the pipette from breaking during the centrifugation process.

[0012] Preferably, an annular convex portion is provided at the inner end of the positioning ring, and correspondingly, an annular concave portion corresponding to the annular convex portion is provided at the end of the pipette.

[0013] The annular protrusion and the annular recess match each other. When the annular protrusion is embedded in the annular recess, it is equivalent to a snap fit, which can further realize the positioning of the pipette.

[0014] Preferably, the containing pipe comprises a lower pipe section and an upper pipe section, and the lower pipe section and the upper pipe section are threadedly connected.

[0015] The lower pipe section and the upper pipe section can be quickly disassembled through screw connection, which is beneficial for cleaning the containing pipe.

[0016] Preferably, an annular threaded section is provided at the lower end of the upper pipe section, and correspondingly, an annular threaded groove is provided at the upper end of the lower pipe section.

[0017] Preferably, a threaded ring is provided at the upper end of the upper pipe section, and correspondingly, a fixing thread is provided on the inner side of the upper cover.

[0018] Compared with the prior art, the beneficial effects of the utility model are:

[0019] 1. The plasma sample can be sucked through the rubber-tipped suction bag, which can achieve a tube of about 1 ml, and can be directly placed in the injection vial for analysis on the machine. There is no need for technicians to observe and measure additionally before transferring to the injection bottle.

[0020] 2. The upper cover is combined with a liquid suction structure, so the staff does not need to use additional liquid suction equipment, which can avoid blood sample contamination caused by external equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 Schematic diagram of the structure of the device for facilitating plasma sampling in a linezolid blood sample (overall);

[0022] Figure 2 Schematic diagram of the structure of the device for facilitating plasma sampling in a linezolid blood sample (cross section);

[0023] Figure 3 It is a schematic diagram of the structure of the upper cover.

[0024] In the figure: 5, rubber-tipped liquid suction bag, 6, upper cover, 7, threaded ring, 8, upper tube section, 9, liquid suction tube, 10, annular convex part, 11, positioning ring, 12, positioning rod, 13, annular threaded section, 14, lower tube section, 15, annular concave part. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0026] Based on the above, this embodiment makes the following supplement from the perspective of application. The device for sampling plasma in a linezolid blood sample mainly includes:

[0027] a receiving tube;

[0028] The receiving tube includes a lower tube section 14 and an upper tube section 8;

[0029] Upper cover 6;

[0030] Rubber head suction bag 5;

[0031] Pipette 9;

[0032] Positioning bracket;

[0033] The positioning bracket includes a positioning rod 12 , a positioning plate and a positioning ring 11 .

[0034] When using the device, the upper cover 6 can be opened first, and after adding the blood sample and the anticoagulant, the upper cover 6 is screwed tight and then placed in a centrifuge for centrifugation.

[0035] After centrifugation is completed, the upper cover 6 can be unscrewed, the pipette can be taken out, and the seal 16 at the end of the pipette can be peeled off. Pinch the rubber head suction bag 5 at the upper end of the upper cover 6, and after the end of the pipette is inserted into the plasma layer, release it, and the pipette will be filled with liquid. Then, by pressing the rubber head suction bag 5, the plasma can be transferred to the injection bottle. At this time, the sample volume is exactly 1ml. The collection and transfer of plasma samples in this way can help improve the efficiency of the experiment and avoid the introduction of impurities by using external suction equipment.

Claims

1. A device for facilitating plasma sampling in a linezolid blood sample, comprising a holding tube for containing sample blood, characterized in that: It comprises an upper cover (6), a rubber-tipped liquid suction bag (5) and a liquid suction tube (9); The upper opening of the accommodating tube is screwed and fixed to the upper cover (6); The upper cover (6) is provided with a liquid through hole, and the rubber head liquid absorption bag (5) is connected to the liquid through hole; The liquid suction tube (9) is fixed to the inner end of the upper cover (6) and is communicated with the liquid through hole.

2. The device for facilitating plasma sampling in a linezolid blood sample according to claim 1, characterized in that: A sealing sticker (16) is provided at one end of the liquid suction tube (9) facing away from the rubber-tip liquid suction bag (5).

3. The device for facilitating plasma sampling in a linezolid blood sample according to claim 1, characterized in that: It also includes a positioning bracket, the positioning bracket including a positioning rod (12), a positioning plate and a positioning ring (11); One end of the positioning rod (12) is obliquely fixed to the inner wall of the containing tube, and the other end is fixed to the positioning plate; The positioning ring (11) is fixed above the positioning plate, and one end of the liquid suction tube (9) facing away from the rubber head liquid suction bag (5) is inserted into the positioning ring (11).

4. The device for facilitating plasma sampling in a linezolid blood sample according to claim 3, characterized in that: An annular convex portion (10) is provided at the inner end of the positioning ring (11), and correspondingly, an annular concave portion (15) corresponding to the annular convex portion (10) is provided at the end of the liquid pipette (9).

5. The device for facilitating plasma sampling in a linezolid blood sample according to claim 1, characterized in that: The accommodating tube comprises a lower tube section (14) and an upper tube section (8), wherein the lower tube section (14) and the upper tube section (8) are screwed together.

6. The device for facilitating plasma sampling in a linezolid blood sample according to claim 5, characterized in that: The lower end of the upper pipe section (8) is provided with an annular thread section (13), and correspondingly, the upper end of the lower pipe section (14) is provided with an annular thread groove.

7. The device for facilitating plasma sampling in a linezolid blood sample according to claim 5, characterized in that: A threaded ring (7) is provided at the upper end of the upper pipe section (8), and correspondingly, a fixing thread is provided on the inner side of the upper cover (6).

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