Full-automatic sample application device for precise microarray type protein chip

Abstract

The utility model provides a full-automatic sample application device for a precise microarray type protein chip, which comprises a supporting table, a movable base table and a flexible film carrying sheet, and is characterized by further comprising an automatic sample application mechanism which comprises a sample application structure, a container for storing protein liquid, an automatic valve switch assembly, a two-dimensional movable assembly and a sample applicator with a liquid outlet hole, the position recognition structure is provided with a position recognition emitter and a plurality of position recognition receivers, and the circuit substrate is electrically connected with the automatic valve switch assembly, the two-dimensional moving assembly, the recognition emitter and the position recognition receivers. Due to the adoption of the structure and the arrangement of the position identification structure in the automatic sample application mechanism, compared with the existing manual sample application, the automatic sample application mechanism has the advantages that the sample application position can be accurately detected, the full-automatic function is realized in the sample application process, the full-automatic sample application is realized, the production efficiency is high, the sample application positioning is accurate, and the consumed time is short.

Description

Technical Field

[0001] The utility model belongs to the technical field of instrument analysis equipment, in particular to a fully automatic spotting device for a precise microarray protein chip. Background Art

[0002] Microarray-based chip analysis technology can achieve high-throughput detection or screening. The chip has developed from the initial DNA chip to protein chip, cell chip, tissue chip, etc., and its detection method has developed from fluorescence imaging to label-free surface plasmon resonance imaging, etc., and has broad application prospects in the fields of omics research, disease diagnosis, and drug screening.

[0003] In the related prior art, for example, an invention patent authorized by China discloses a microarray spotting device, with a publication number of CN103712956B, and specifically discloses "a microarray spotting device, characterized in that the device comprises a bottom plate (1), a spotting unit (2), a substrate fixing adjustment unit (3) and a micro-sample storage and transport unit (4); wherein the spotting unit (2) comprises a spotting needle (5) and a spotting needle fixing adjustment mechanism, and the spotting needle fixing adjustment mechanism comprises a fixed on the bottom plate (1) The invention relates to a vertical rod (6) on the substrate (14) to be spotted, an axis (7) fixed on the vertical rod, a cross rod (8) capable of rotating about the axis (7), and a limiting component (9) fixed on the vertical rod (6); the limiting component (9) limits the position of the cross rod (8) relative to the substrate (14) to be spotted by adjusting a round head screw (10) arranged therein, wherein the cross rod (8) is in a horizontal position in a natural state; a vertical groove is provided on the cross rod (8) for accommodating a spotting needle (5), and a fixing block (11) fixes the spotting needle (5) on the cross rod (8) by pressure. ), the sample spotting needle does not contact the substrate (14) to be spotted; the sample spotting needle is a section of capillary; the substrate fixing adjustment unit (3) comprises a two-dimensional translation platform (15) and substrate fixing components (16, 17) located thereon, the two-dimensional translation platform (15) is fixed parallel to the bottom plate (1), the substrate fixing components (16, 17) are two identical strip-shaped fixing blocks, the position of which can be adjusted up and down, and the substrate (14) to be spotted is clamped and fixed on the two-dimensional translation platform (15); the micro sample storage and transportation unit (4) comprises a micro The invention relates to a micro-sample sealed storage bottle (12) and a pressurizing device, wherein the non-spotting end of the spotting needle (5) is inserted into the bottom of the micro-sample sealed storage bottle (12), the pressurizing device is connected to the micro-sample sealed storage bottle (12) through a pipeline (13), and the micro-sample sealed storage bottle (12) is fixed on the bottom plate (1). In the spotting process, the spotting device may not align the spotting sample with the substrate, and the alignment needs to be repeatedly fine-tuned manually by feeling, which leads to technical problems such as low production efficiency, inaccurate spotting positioning, and long time consumption.

[0004] To this end, the utility model provides a fully automatic spotting device for a precise microarray protein chip. Utility Model Content

[0005] In view of the above problems existing in the prior art, the purpose of the present invention is to provide a fully automatic spotting device for precise microarray protein chips, which has the advantages of fully automatic spotting, high production efficiency, precise spotting positioning, and short time consumption.

[0006] The purpose of the utility model can be achieved through the following technical solutions:

[0007] A fully automatic spotting device for precise microarray protein chips, comprising

[0008] Support table;

[0009] A movable base is arranged on the supporting platform, and a receiving groove with an upper opening is provided on the top of the movable base;

[0010] A flexible film carrier sheet, the peripheral edge of which is embedded in the containing groove, and the flexible film carrier sheet has a plurality of array-distributed spotting grooves for placing the protein solution;

[0011] It also includes an automatic spotting mechanism, which is arranged above the mobile base and includes

[0012] The sample spotting structure comprises a container for storing protein solution, an automatic valve switch component, a two-dimensional moving component and a sample spotter with a liquid outlet, wherein the two-dimensional moving component can drive the sample spotter to move in the up-down and left-right directions, the sample spotter is connected with the container through a tube body, the automatic valve switch component is arranged in the pipeline and is used to control the on-off of the flow of the protein solution, and the sample spotter has an inclined wall portion;

[0013] A position identification structure, comprising a position identification transmitter and a plurality of position identification receivers, wherein the position identification transmitter is embedded in the outer side wall of the inclined wall portion, and the plurality of position receivers are embedded in the outer peripheral edge of the top of the spotting slot;

[0014] The circuit substrate is electrically connected to the automatic valve switch component, the two-dimensional moving component, the identification transmitter and the position identification receiver respectively.

[0015] As a preferred technical solution of the utility model, the two-dimensional moving component includes

[0016] An up-and-down moving member, comprising a longitudinal frame plate, a first longitudinal guide rail, a second longitudinal guide rail, a transverse plate, a rotating arm and a first motor, wherein the first longitudinal guide rail and the second longitudinal guide rail are respectively fixed to the longitudinal frame plate in the longitudinal direction, and two ends of the transverse plate are slidably arranged between the first longitudinal guide rail and the second longitudinal guide rail in the up-and-down direction; an output end of the first motor is connected to the transverse plate through the rotating arm;

[0017] The left-right moving member includes a connecting arm and a second motor, wherein the second motor is connected to the end of the horizontal plate through the connecting arm, the bases of the first motor and the second motor are respectively fixed to the longitudinal frame plate, and the signal ends of the first motor and the second motor are respectively electrically connected to the circuit substrate.

[0018] As a preferred technical solution of the present utility model, the automatic valve switch assembly includes an electric valve.

[0019] As a preferred technical solution of the utility model, the spotter includes a steel needle, and the steel needle is an integral part made of stainless steel.

[0020] As a preferred technical solution of the utility model, the spotter includes a gun head, and the gun head is an integral part made of stainless steel.

[0021] As a preferred technical solution of the utility model, it also includes a quantitative liquid injection mechanism, which includes a quantitative device, the inlet of the quantitative device is connected to the container through a tube body, the outlet of the quantitative device is connected to the automatic valve switch assembly through another tube body, and the signal end of the quantitative device is electrically connected to the circuit substrate.

[0022] As described above, the fully automatic spotting device for precise microarray protein chips provided by the present invention has the following beneficial effects:

[0023] The utility model utilizes the above-mentioned fully automatic spotting device of the precise microarray protein chip. Compared with the prior art, due to the adoption of such a structure, a position recognition structure in the automatic spotting mechanism is set. Compared with the prior manual spotting, the position recognition transmitter and the position recognition receiver of the position recognition structure can be used to accurately detect the spotting position, and the spotting process realizes a fully automated function, which has the advantages of fully automatic spotting, high production efficiency, precise spotting positioning, and short time consumption. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a schematic diagram of the structure of the fully automatic spotting device for the precise microarray protein chip applied for by the utility model;

[0025] Figure 2This is a control structure block diagram of the fully automatic spotting device for the precise microarray protein chip applied for by the utility model. DETAILED DESCRIPTION

[0026] The following is a description of the implementation of the present invention by means of specific embodiments. People familiar with the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification.

[0027] It should be noted that the structures, proportions, sizes, etc. illustrated in the drawings of this specification are only used to match the contents disclosed in the specification for people familiar with this technology to understand and read, and are not used to limit the limiting conditions that can be implemented in this utility model, so they have no substantial technical significance. Any modification of the structure, change of the proportional relationship or adjustment of the size, without affecting the effects that can be produced by the utility model and the purposes that can be achieved, should still fall within the scope of the technical content disclosed in this utility model. At the same time, the terms such as "upper", "lower", "left", "right", "middle" and "one" quoted in this specification are only for the convenience of description, and are not used to limit the scope of the implementation of this utility model. The change or adjustment of their relative relationship, without substantial change in the technical content, should also be regarded as the scope of the implementation of this utility model. The specific structure can be described with reference to the drawings of the patent application.

[0028] Example 1

[0029] The utility model provides a fully automatic spotting device for precise microarray protein chips. Figure 1 and Figure 2 As shown, it includes a support platform 1;

[0030] A movable base 2 is disposed on the supporting platform 1, and a receiving groove 20 with an upper opening is provided on the top of the movable base 2;

[0031] A flexible film carrier sheet 3, whose peripheral edge is embedded in the containing groove 20, and the flexible film carrier sheet 3 has a plurality of array-distributed spotting grooves 30 for placing the protein solution;

[0032] It also includes an automatic spotting mechanism 4, which is arranged above the mobile base 2 and includes

[0033] The sample spotting structure comprises a container 40 for storing protein solution, an automatic valve switch component 41, a two-dimensional moving component 42 and a sample spotter 43 having a liquid outlet, wherein the two-dimensional moving component 41 can drive the sample spotter 43 to move in the up-down and left-right directions, the sample spotter 43 is connected with the container 40 through a tube body, the automatic valve switch component 41 is arranged in the pipeline and is used to control the on-off of the flow of the protein solution, and the sample spotter 43 has an inclined wall portion 430;

[0034] The position identification structure 44 has a position identification transmitter 440 and a plurality of position identification receivers 441, wherein the position identification transmitter 440 is embedded in the outer wall of the inclined wall portion 430, and the plurality of position receivers 441 are embedded in the outer peripheral edge of the top of the spotting slot 30;

[0035] The circuit substrate 45 is electrically connected to the automatic valve switch component 41 , the two-dimensional moving component 42 , the identification transmitter 440 and the position identification receiver 441 , respectively.

[0036] When in use, the flexible film carrier sheet 3 to be spotted is first placed in the upper opening of the containing groove 20; then, under the action of the automatic spotting mechanism 4, the two-dimensional moving component 42 is used to move the spotter 43 from top to bottom and left to right. When the position recognition transmitter 440 disposed on the inclined wall portion 430 of the spotter 43 is aligned with the position receiver 441 disposed at the spotting groove 30, the circuit substrate 45 can receive the position precision alignment signal generated between the position recognition transmitter 440 and the position receiver 441. Then, the circuit substrate 45 starts the automatic valve switch assembly 41 to open, so that the flow path of the protein solution is in a cleared state, so that the protein solution stored in the container is tapped into the corresponding spotting groove 30 from the port of the spotter 43, realizing non-contact spotting, and the spotting position can be accurately detected. The spotting process realizes a fully automated function, with the advantages of fully automatic spotting, high production efficiency, accurate spotting positioning, and short time consumption; and the spotting process is realized by a non-contact structure, and there is no contact pollution in the entire spotting process, and the spotting process is clean and hygienic.

[0037] Combination Figure 1 As shown, in this embodiment, the two-dimensional moving component 42 includes

[0038] The up-and-down moving member includes a longitudinal frame plate 420, a first longitudinal guide rail 421, a second longitudinal guide rail 422, a transverse plate 423, a rotating arm 424, and a first motor 425. The first longitudinal guide rail 421 and the second longitudinal guide rail 422 are respectively fixed to the longitudinal frame plate 420 in the longitudinal direction, and both ends of the transverse plate 423 are slidably arranged between the first longitudinal guide rail 421 and the second longitudinal guide rail 422 in the up-and-down direction; the output end of the first motor 425 is connected to the transverse plate 423 through the rotating arm 424;

[0039] The left and right moving parts include a connecting arm 426 and a second motor 427. The second motor 427 is connected to the end of the horizontal plate 423 through the connecting arm 426. The bases of the first motor 425 and the second motor 427 are respectively fixed to the longitudinal frame plate 420. The signal ends of the first motor 425 and the second motor 427 are respectively electrically connected to the circuit substrate 45.

[0040] In this embodiment, the automatic valve switch assembly 41 includes an electric valve.

[0041] In this embodiment, the spotter 43 includes a steel needle, and the steel needle is an integral part made of stainless steel.

[0042] In this embodiment, the spotter 43 includes a gun head, and the gun head is an integral part made of stainless steel.

[0043] Example 2

[0044] Based on Example 1, Figure 1 and Figure 2 As shown, in this embodiment, a quantitative injection mechanism 5 is also included, and the quantitative injection mechanism 5 includes a quantitative device 50, the inlet of the quantitative device 50 is connected to the container 40 through a tube body, the outlet of the quantitative device 50 is connected to the automatic valve switch assembly 41 through another tube body, and the signal end of the quantitative device 50 is electrically connected to the circuit substrate 45. By providing the quantitative device 50, the protein solution can be quantitatively input into the spotting groove 30, preventing the influence of too much or too little protein solution on the subsequent chip analysis from not reaching the standard, so that the device of the present application has the function of quantitatively inputting protein solution, and is easy to use.

[0045] As described above, the fully automatic spotting device for precise microarray protein chips provided by the present invention has the following beneficial effects:

[0046] The utility model utilizes the above-mentioned fully automatic spotting device of the precise microarray protein chip. Compared with the prior art, due to the adoption of such a structure, a position recognition structure in the automatic spotting mechanism is set. Compared with the prior manual spotting, the position recognition transmitter and the position recognition receiver of the position recognition structure can be used to accurately detect the spotting position, and the spotting process realizes a fully automated function, which has the advantages of fully automatic spotting, high production efficiency, precise spotting positioning, and short time consumption.

[0047] The above is only a preferred implementation of the present invention. It should be noted that, for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, which are considered to be within the protection scope of the present invention.

Claims

1. A fully automatic spotting device for precise microarray protein chips, comprising: Support table; A movable base is arranged on the supporting platform, and a receiving groove with an upper opening is provided on the top of the movable base; A flexible film carrier sheet, the peripheral edge of which is embedded in the containing groove, and the flexible film carrier sheet has a plurality of array-distributed spotting grooves for placing the protein solution; It is characterized by: it also includes The automatic spotting mechanism is arranged above the mobile base and comprises The sample spotting structure comprises a container for storing protein solution, an automatic valve switch component, a two-dimensional moving component and a sample spotter with a liquid outlet, wherein the two-dimensional moving component can drive the sample spotter to move in the up-down and left-right directions, the sample spotter is connected with the container through a tube body, the automatic valve switch component is arranged in the pipeline and is used to control the on-off of the flow of the protein solution, and the sample spotter has an inclined wall portion; A position identification structure, comprising a position identification transmitter and a plurality of position identification receivers, wherein the position identification transmitter is embedded in the outer side wall of the inclined wall portion, and the plurality of position receivers are embedded in the outer peripheral edge of the top of the spotting slot; The circuit substrate is electrically connected to the automatic valve switch component, the two-dimensional moving component, the identification transmitter and the position identification receiver respectively.

2. The fully automatic spotting device for a precise microarray protein chip according to claim 1, characterized in that: The two-dimensional moving component includes An up-and-down moving member, comprising a longitudinal frame plate, a first longitudinal guide rail, a second longitudinal guide rail, a transverse plate, a rotating arm and a first motor, wherein the first longitudinal guide rail and the second longitudinal guide rail are respectively fixed to the longitudinal frame plate in the longitudinal direction, and two ends of the transverse plate are slidably arranged between the first longitudinal guide rail and the second longitudinal guide rail in the up-and-down direction; an output end of the first motor is connected to the transverse plate through the rotating arm; The left-right moving member includes a connecting arm and a second motor, wherein the second motor is connected to the end of the horizontal plate through the connecting arm, the bases of the first motor and the second motor are respectively fixed to the longitudinal frame plate, and the signal ends of the first motor and the second motor are respectively electrically connected to the circuit substrate.

3. The fully automatic spotting device for a precise microarray protein chip according to claim 1, characterized in that: The automatic valve switch assembly includes an electric valve.

4. A fully automatic spotting device for a precise microarray protein chip according to any one of claims 1 to 3, characterized in that: The spotter comprises a steel needle, and the steel needle is an integral part made of stainless steel.

5. A fully automatic spotting device for a precise microarray protein chip according to any one of claims 1 to 3, characterized in that: The spotter comprises a gun head, and the gun head is an integral part made of stainless steel.

6. A fully automatic spotting device for a precise microarray protein chip according to any one of claims 1 to 3, characterized in that: It also includes a quantitative liquid injection mechanism, which includes a doser, whose inlet is connected to the container through a tube, whose outlet is connected to the automatic valve switch assembly through another tube, and whose signal end is electrically connected to the circuit substrate.

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