Environment control device for umbilical cord mesenchymal stem cell culture

Abstract

The utility model discloses an environment control device for umbilical cord mesenchymal stem cell culture, which is characterized in that the lower surface of a bottom plate is fixedly connected with a bracket, the interior of the bracket is rotatably connected with a roller, the upper surface of the bottom plate is fixedly connected with a shell, the left surface of the shell is fixedly connected with a gas tank A, and the left surface of the shell is fixedly connected with a gas tank B; the upper surfaces of the gas tank A and the gas tank B are fixedly connected with a conveying pipe, the side surface of the conveying pipe is provided with a valve, the left inner wall of the shell is fixedly connected with a partition plate, the upper surface of the partition plate is movably connected with a placing plate, the upper surface of the partition plate is fixedly connected with high-transmittance ultraviolet glass, and the upper surface of the shell is fixedly connected with a lamp holder. An ultraviolet lamp is fixedly connected to the lower surface of the lamp holder, a heater is fixedly connected to the right surface of the shell, and a heat conduction rod is fixedly connected to the output end of the heater, through the devices, control over internal gas is facilitated, and cells have different culture environments.

Description

Technical Field

[0001] The utility model relates to the technical field of stem cell culture, in particular to an environment control device for umbilical cord mesenchymal stem cell culture. Background Art

[0002] Umbilical cord mesenchymal stem cells refer to a type of multifunctional stem cells present in the umbilical cord tissue of newborns. They can differentiate into many types of tissue cells and have broad clinical application prospects. The environmental control device for umbilical cord mesenchymal stem cell culture is a device for providing a suitable environment to support the growth and culture of umbilical cord mesenchymal stem cells. However, the existing device does not isolate the sterilization equipment, which may affect the sterilization lamps during the culture process and is not conducive to cleaning. For example, a Chinese patent discloses "an umbilical cord mesenchymal stem cell incubator" with an application number of "202120378324.4". It uses ultraviolet disinfection lamps to disinfect the box to ensure that the box remains sterile and prevent contamination during the culture of umbilical cord mesenchymal stem cells. However, the disinfection lamps are not isolated from the culture tubes, and the gases generated during the culture process may affect the lamps. Utility Model Content

[0003] The utility model aims to solve at least one of the technical problems existing in the prior art, and provides an umbilical cord mesenchymal stem cell culture environment control device. The interior of the shell can be divided into different spaces by the arrangement of a partition and a high-transmittance ultraviolet glass. A placement plate is arranged on the partition to prevent the culture solution or the generated gas from affecting the ultraviolet lamp and the heat-conducting rod, and at the same time collects impurities that overflow or are generated during the culture process, which is beneficial to extending the service life of the equipment and ensuring the cleanliness of the interior of the device. The oxygen and carbon dioxide in the gas tank A and the gas tank B are delivered into the shell through a delivery pipe, and the gas flow is controlled by a valve. Oxygen and carbon dioxide can be input simultaneously or individually, which is beneficial to conveniently adjust different gas concentrations during the culture process at any time.

[0004] The utility model also provides an environment control device for culturing umbilical cord mesenchymal stem cells, comprising: a bottom plate, a bracket fixedly connected to the lower surface of the bottom plate, a roller rotatably connected to the inside of the bracket, a shell fixedly connected to the upper surface of the bottom plate, a gas tank A fixedly connected to the left surface of the shell, a gas tank B fixedly connected to the left surface of the shell, a delivery pipe fixedly connected to the upper surfaces of the gas tank A and the gas tank B, a valve is provided on the side surface of the delivery pipe, a partition is fixedly connected to the left inner wall of the shell, a placement plate is movably connected to the upper surface of the partition, a groove is provided on the placement plate, a high-transmittance ultraviolet glass is fixedly connected to the upper surface of the partition, a lamp holder is fixedly connected to the upper surface of the shell, an ultraviolet lamp is fixedly connected to the lower surface of the lamp holder, a heater is fixedly connected to the right surface of the shell, and a heat conducting rod is fixedly connected to the output end of the heater. The above device is beneficial to control the generation of different gas concentration environments and ensure smooth cultivation.

[0005] According to the environmental control device for culturing umbilical cord mesenchymal stem cells described in the utility model, the gas tank A and the gas tank B are connected to the outer shell through a delivery pipe, and the valve is located on the left surface of the outer shell. Through the above device, it is beneficial to allow the gas to be stably delivered from the gas tank into the outer shell.

[0006] According to the environmental control device for culturing umbilical cord mesenchymal stem cells described in the utility model, the gas tank A contains oxygen, and the gas tank B contains carbon dioxide. The above device is beneficial for simulating different gas environments.

[0007] According to the environmental control device for culturing umbilical cord mesenchymal stem cells described in the utility model, the heat conducting rod penetrates the outer shell and extends to the inside of the outer shell. The heat conducting rod is located directly below the partition. The above device is beneficial to improving the accuracy of temperature control.

[0008] According to the environmental control device for culturing umbilical cord mesenchymal stem cells described in the utility model, the shell is rotatably connected to a door via a hinge, and the door is located directly in front of the shell. The above device is beneficial to form a relatively closed space.

[0009] According to the environmental control device for culturing umbilical cord mesenchymal stem cells described in the utility model, the gas tank A, the gas tank B and the heater are all located on the upper surface of the bottom plate. The above device is beneficial to facilitate the overall movement of the device.

[0010] According to the environmental control device for culturing umbilical cord mesenchymal stem cells described in the utility model, the ultraviolet lamp penetrates the shell and extends to the inside of the shell. The ultraviolet lamp is located directly behind the high ultraviolet transmittance glass. The above device is beneficial to the activation of the protective effect of the ultraviolet lamp.

[0011] According to the environmental control device for culturing umbilical cord mesenchymal stem cells described in the utility model, a pull ring is fixedly connected to the front surface of the box door, and a sealing gasket is fixedly connected to the rear surface of the box door. The above device is beneficial for facilitating the opening of the box door and ensuring the sealing of the outer shell.

[0012] Beneficial Effects

[0013] 1. Compared with the prior art, the environmental control device for culturing umbilical cord mesenchymal stem cells can divide the interior of the shell into different spaces by setting partitions and high-ultraviolet-transmittance glass. A placement plate is set on the partition to prevent the culture solution or the generated gas from affecting the ultraviolet lamp and the heat-conducting rod, and at the same time collect impurities that overflow or are generated during the culture process, which is beneficial to extending the service life of the equipment and ensuring the cleanliness of the interior of the device.

[0014] 2. Compared with the prior art, in the environmental control device for culturing umbilical cord mesenchymal stem cells, the oxygen and carbon dioxide in gas tanks A and B are delivered into the outer shell through a delivery pipe, and the gas flow is controlled by a valve. Oxygen and carbon dioxide can be input simultaneously or individually, which is beneficial for conveniently adjusting different gas concentrations during the culture process at any time and avoiding gas waste. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The utility model is further described below in conjunction with the accompanying drawings and embodiments;

[0016] Figure 1 This is a three-dimensional structural diagram of the environment control device for culturing umbilical cord mesenchymal stem cells of the utility model;

[0017] Figure 2 This is a front cross-sectional structural diagram of the environment control device for culturing umbilical cord mesenchymal stem cells of the utility model;

[0018] Figure 3 It is a right side cross-sectional structural diagram of the environment control device for culturing umbilical cord mesenchymal stem cells of the utility model;

[0019] Figure 4 This is a bottom view of the structure of the environment control device for culturing umbilical cord mesenchymal stem cells of the present invention.

[0020] Legend:

[0021] 1. Outer shell; 2. Bottom plate; 3. Delivery pipe; 4. Valve; 5. Gas tank A; 6. Gas tank B; 7. Box door; 8. Pull ring; 9. Heater; 10. Heat conducting rod; 11. Partition; 12. Placement plate; 13. High-transmittance UV glass; 14. Bracket; 15. Roller; 16. Lamp holder; 17. UV lamp; 18. Sealing gasket. DETAILED DESCRIPTION

[0022] This section will describe in detail the specific embodiments of the utility model. The preferred embodiments of the utility model are shown in the accompanying drawings. The purpose of the accompanying drawings is to supplement the description of the text part of the specification with graphics, so that people can intuitively and vividly understand each technical feature and the overall technical solution of the utility model, but it cannot be understood as a limitation on the protection scope of the utility model.

[0023] Reference Figure 1-4 The utility model embodiment is an umbilical cord mesenchymal stem cell culture environment control device, which includes: a bottom plate 2, the lower surface of the bottom plate 2 is fixedly connected with a bracket 14, which is used to connect a roller 15, the bracket 14 is internally rotatably connected with the roller 15, which is used to ensure the smooth movement of the device, the upper surface of the bottom plate 2 is fixedly connected with a shell 1, the left surface of the shell 1 is fixedly connected with a gas tank A5, the gas tank A5 contains oxygen, which is used to improve the environmental concentration of oxygen during the culture process, the left surface of the shell 1 is fixedly connected with a gas tank B6, the gas tank B6 contains carbon dioxide, which is used to improve the concentration of carbon dioxide during the culture process. The environmental concentration of carbon monoxide is adjusted, and a delivery pipe 3 is fixedly connected to the upper surface of the gas tank A5 and the gas tank B6 for delivering gas. A valve 4 is provided on the side surface of the delivery pipe 3 for controlling the delivery flow rate. The gas tank A5 and the gas tank B6 are connected with the outer shell 1 through the delivery pipe 3, and the valve 4 is located on the left surface of the outer shell 1. A partition 11 is fixedly connected to the left inner wall of the outer shell 1 for avoiding the influence of operating errors on the heat-conducting rod 10 during the culture process. A placement plate 12 is movably connected to the upper surface of the partition 11 for placing a culture dish. A groove is provided on the placement plate 12 for collecting overflowed culture solution.

[0024] A high-transmittance UV glass 13 is fixedly connected to the upper surface of the partition 11 for isolating the UV lamp 17. A lamp holder 16 is fixedly connected to the upper surface of the shell 1 for installing the UV lamp 17. The lower surface of the lamp holder 16 is fixedly connected to the UV lamp 17 for sterilization. The UV lamp 17 penetrates the shell 1 and extends to the interior of the shell 1. The UV lamp 17 is located directly behind the high-transmittance UV glass 13. A heater 9 is fixedly connected to the right surface of the shell 1 for generating heat. A heat-conducting rod 10 is fixedly connected to the output end of the heater 9 for transmitting heat. The heat-conducting rod 10 penetrates the shell 1 and extends to the interior of the shell 1. The heat-conducting rod 10 is located directly below the partition 11. The shell 1 is rotatably connected to a box door 7 through a hinge for closing the shell 1. The box door 7 is located directly in front of the shell 1. A pull ring 8 is fixedly connected to the front surface of the box door 7 for facilitating the opening and closing of the box door 7. A sealing gasket 18 is fixedly connected to the rear surface of the box door 7 for ensuring the sealing of the sealed shell 1.

[0025] Working principle: gas tank A5 and gas tank B6 store oxygen and carbon dioxide respectively. Through the control of valve 4, gas can be sent into shell 1 through delivery pipe 3. Valve 4 can adjust the flow of gas, so as to control the gas concentration inside shell 1. Heater 9 heats gas inside shell 1 through heat conducting rod 10 to ensure the required gas temperature. The design of heat conducting rod 10 enables heat to be evenly transferred to improve the heating effect. Ultraviolet lamp 17 emits ultraviolet light, which is filtered by high ultraviolet glass 13 to sterilize and disinfect gas inside shell 1, and also protect ultraviolet lamp 17. Culture dish is placed on placement board 12, and placement board 12 can be placed on partition 11 movably to facilitate cleaning of internal environment. Box door 7 is connected to shell 1 through hinge for easy opening and closing. Pull ring 8 is fixed on the front surface of box door 7 for easy operation. Sealing pad 18 is fixed on the rear surface of box door 7 to ensure the sealing of box door 7 when it is closed to prevent gas leakage and affect the health of the culture staff.

[0026] The embodiments of the present invention are described in detail above in conjunction with the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge scope of ordinary technicians in the relevant technical field without departing from the purpose of the present invention.

Claims

1. An environmental control device for culturing umbilical cord mesenchymal stem cells, characterized in that: include: A bottom plate (2), a bracket (14) is fixedly connected to the lower surface of the bottom plate (2), a roller (15) is rotatably connected inside the bracket (14), a housing (1) is fixedly connected to the upper surface of the bottom plate (2), a gas tank A (5) is fixedly connected to the left surface of the housing (1), a gas tank B (6) is fixedly connected to the left surface of the housing (1), a delivery pipe (3) is fixedly connected to the upper surfaces of the gas tank A (5) and the gas tank B (6), a valve (4) is provided on the side surface of the delivery pipe (3), and the left surface of the housing (1) is fixedly connected to the upper surface of the gas tank A (5) and the gas tank B (6). A partition (11) is fixedly connected to the inner wall, a placement plate (12) is movably connected to the upper surface of the partition (11), a groove is provided on the placement plate (12), a high-transmittance ultraviolet glass (13) is fixedly connected to the upper surface of the partition (11), a lamp holder (16) is fixedly connected to the upper surface of the outer shell (1), an ultraviolet lamp (17) is fixedly connected to the lower surface of the lamp holder (16), a heater (9) is fixedly connected to the right surface of the outer shell (1), and a heat conducting rod (10) is fixedly connected to the output end of the heater (9).

2. The environmental control device for culturing umbilical cord mesenchymal stem cells according to claim 1, characterized in that: The gas tank A (5) and the gas tank B (6) are both connected to the outer shell (1) through the delivery pipe (3), and the valve (4) is located on the left surface of the outer shell (1).

3. The environmental control device for culturing umbilical cord mesenchymal stem cells according to claim 1, characterized in that: The gas tank A (5) contains oxygen, and the gas tank B (6) contains carbon dioxide.

4. The environmental control device for culturing umbilical cord mesenchymal stem cells according to claim 1, characterized in that: The heat conducting rod (10) passes through the outer shell (1) and extends to the interior of the outer shell (1); the heat conducting rod (10) is located directly below the partition (11).

5. The environmental control device for culturing umbilical cord mesenchymal stem cells according to claim 1, characterized in that: The housing (1) is rotatably connected to a door (7) via a hinge, and the door (7) is located directly in front of the housing (1).

6. The environmental control device for culturing umbilical cord mesenchymal stem cells according to claim 1, characterized in that: The gas tank A (5), the gas tank B (6) and the heater (9) are all located on the upper surface of the bottom plate (2).

7. The environmental control device for culturing umbilical cord mesenchymal stem cells according to claim 1, characterized in that: The ultraviolet lamp (17) penetrates the housing (1) and extends to the interior of the housing (1); the ultraviolet lamp (17) is located directly behind the high-transmittance ultraviolet glass (13).

8. The environmental control device for culturing umbilical cord mesenchymal stem cells according to claim 5, characterized in that: A pull ring (8) is fixedly connected to the front surface of the box door (7), and a sealing gasket (18) is fixedly connected to the rear surface of the box door (7).

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