Cell incubator for biological cell research

Abstract

The utility model discloses a cell culture box for biological cell research, which comprises a culture box, a mounting plate, culture plates and a mounting column, the mounting plate is mounted in the culture box, the mounting column is mounted in the middle of the top of the mounting plate, a heating cavity is arranged in the mounting column, the culture plates are uniformly mounted on the outer side of the mounting column, and the mounting plate is mounted on the mounting column. Heating grooves are formed in the ends, away from the mounting column, of the culture plates, and a first air extracting pump is mounted at one end of the bottom of the mounting plate. The device is provided with the culture plates, the first air pump, the electric heating wire, the mounting column and the communicating pipes, and is provided with the heating grooves and the heating cavity, so that when the device is used, hot air enters the heating cavity through the heating pipes and is shunted into the heating grooves in the culture plates through the communicating pipes; the heat can be uniformly transferred to different culture dishes through the culture plate, so that the temperature of the culture dishes can be kept uniform.

Description

Technical Field

[0001] The utility model relates to the technical field of biological cell culture equipment, in particular to a cell culture box for biological cell research. Background Art

[0002] Biological cells are a type of cells that have unlimited or immortal self-renewal capabilities and can produce at least one type of highly differentiated daughter cells. When studying biological cells, the biological cells need to be placed in a culture dish, and then all the culture dishes are placed inside an incubator, which provides suitable temperature and humidity to culture the biological cells.

[0003] For example, the utility model: "A cell culture box for biological cell research", with the announcement number: "CN211689102U", can achieve the purpose of good humidification effect through the coordinated use of the incubator body, the first motor, the second motor, the stirring shaft and the stirring rod, so that the interior of the incubator body can be cooled down when the temperature is too high, and the humidity inside the incubator body can be maintained on the other hand, thereby creating a good environment for cell culture and being more conducive to cell culture. However, during the implementation of this patent, the heating tube is installed inside the incubator body. During the heating process, it is not easy to evenly transfer heat to the culture dishes at different positions. Moreover, during the implementation of this patent, the temperature and humidity inside the incubator body cannot be automatically adjusted. Its intelligence level is low and it is inconvenient to use. Utility Model Content

[0004] The purpose of the utility model is to provide a cell culture box for biological cell research to solve the problems raised in the above background technology.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a cell culture box for biological cell research, comprising a culture box, a mounting plate, a culture plate and a mounting column, wherein the interior of the culture box is installed with a mounting plate, and a mounting column is installed at a middle position on the top of the mounting plate, a heating cavity is arranged inside the mounting column, and culture plates are evenly installed on the outside of the mounting column, and the ends of the culture plates away from the mounting column are all provided with heating grooves, a first vacuum pump is installed at one end of the bottom of the mounting plate, an vacuum pipe extending to the outside of the culture box is installed at the input end of the first vacuum pump, and a heating pipe is installed at the output end of the first vacuum pump, the heating pipe passes through the mounting plate and extends to the inside of the heating cavity, and the heating pipe An electric heating wire is wound around the outside, a humidifying water tank is installed at one end of the bottom of the incubator away from the first air pump, a second air pump is installed at one end of the bottom of the mounting plate away from the first air pump, a humidity sensor is installed at the top of the incubator near one end of the first air pump, and a temperature sensor is installed at the top of the incubator away from the humidity sensor, a single-chip microcomputer is installed on the top of the incubator, an output end of the humidity sensor is electrically connected to an input end of the single-chip microcomputer through a wire, an output end of the temperature sensor is electrically connected to an input end of the single-chip microcomputer through a wire, and an output end of the single-chip microcomputer is electrically connected to the first air pump, the electric heating wire and the input end of the second air pump respectively through a wire.

[0006] Preferably, culture dish placement grooves are evenly arranged on the top of the culture plate, and culture dishes are installed inside the culture dish placement grooves.

[0007] Preferably, connecting tubes are evenly installed on the outer side of the mounting column, and the connecting tubes are all connected to the heating chamber, and the connecting tubes all pass through the culture plate and extend to the interior of the heating tank.

[0008] Preferably, a humidifying tube is installed at one end of the humidifying water tank away from the second vacuum pump, and the humidifying tube passes through the incubator and extends to the interior of the incubator.

[0009] Preferably, an air outlet pipe extending to the interior of the humidification water tank is installed at the output end of the second air pump, and an air guide pipe is installed at the input end of the second air pump, and the air guide pipe passes through the mounting plate.

[0010] Preferably, an air duct is provided at the top of the incubator at one end away from the temperature sensor, and a dustproof net is installed inside the air duct.

[0011] Preferably, a door is installed on one side of the incubator, and a handle is installed on the outer side of the door.

[0012] Compared with the prior art, the beneficial effects of the utility model are:

[0013] 1. The cell culture box for biological cell research is equipped with a culture plate, a first vacuum pump, an electric heating wire, a mounting column and a connecting pipe, and is provided with a heating tank and a heating chamber. When in use, hot air enters the heating chamber through the heating pipe, and is diverted to the heating tanks on multiple groups of culture plates through multiple groups of connecting pipes, so that heat can be evenly transferred to different culture dishes through the culture plate, which is conducive to keeping the temperature of the culture dishes uniform;

[0014] 2. The cell culture box for biological cell research is equipped with a humidifying tube, a humidifying water tank and a second vacuum pump. During use, the second vacuum pump draws the gas inside the culture box into the humidifying water tank, and the gas can be humidified by the water inside the humidifying water tank, and the gas is blown back into the culture box through the humidifying tube, so that the humidity inside the culture box can be adjusted;

[0015] 3. The cell culture incubator for biological cell research is equipped with a humidity sensor, a single-chip microcomputer and a temperature sensor. During use, the humidity sensor and the temperature sensor monitor the humidity and temperature inside the incubator respectively, and transmit the monitored information to the single-chip microcomputer. The single-chip microcomputer can control the first vacuum pump, the electric heating wire and the second vacuum pump, and can automatically adjust the temperature and humidity inside the incubator. The device has a high degree of automation and is relatively convenient to use. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the front cross-sectional view of the utility model;

[0017] Figure 2 It is a main schematic diagram of the utility model;

[0018] Figure 3 This is a circuit control schematic diagram of the utility model.

[0019] In the figure: 1. incubator; 2. humidifying tube; 3. exhaust tube; 4. mounting plate; 5. culture plate; 6. humidity sensor; 7. culture dish; 8. heating tank; 9. air outlet; 10. culture dish placement tank; 11. first exhaust pump; 12. electric heating wire; 13. heating tube; 14. humidifying water tank; 15. exhaust pipe; 16. second exhaust pump; 17. air guide tube; 18. single chip microcomputer; 19. mounting column; 20. heating chamber; 21. temperature sensor; 22. connecting pipe; 23. box door. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0021] See also Figure 1-3 , an embodiment provided by the utility model: a cell culture box for biological cell research, comprising a culture box 1, a mounting plate 4, a culture plate 5 and a mounting column 19, the interior of the culture box 1 is installed with a mounting plate 4, and a mounting column 19 is installed at the middle position of the top of the mounting plate 4, a heating chamber 20 is arranged inside the mounting column 19, and culture plates 5 are evenly installed on the outside of the mounting column 19, and the ends of the culture plates 5 away from the mounting column 19 are all provided with heating grooves 8, a first air pump 11 is installed at one end of the bottom of the mounting plate 4, the model of the first air pump 11 here can be VCH1028, an air extraction pipe 3 extending to the outside of the culture box 1 is installed at the input end of the first air extraction pump 11, and a heating pipe 13 is installed at the output end of the first air extraction pump 11, the heating pipe 13 passes through the mounting plate 4 and extends to the inside of the heating chamber 20, and an electric heating wire 12 is wound around the outside of the heating pipe 13, the bottom of the interior of the culture box 1 away from the first air extraction pump 11 A humidifying water tank 14 is installed at one end, a second air pump 16 is installed at the end of the bottom of the mounting plate 4 away from the first air pump 11, and the model of the second air pump 16 here can be VCH1028, a humidity sensor 6 is installed at the top of the incubator 1 near the first air pump 11, and the model of the humidity sensor 6 here can be DHT11, a temperature sensor 21 is installed at the top of the incubator 1 away from the humidity sensor 6, and the model of the temperature sensor 21 here can be CWDZ11, a single-chip microcomputer 18 is installed on the top of the incubator 1, and the model of the single-chip microcomputer 18 here can be HT66F018, the output end of the humidity sensor 6 is electrically connected to the input end of the single-chip microcomputer 18 through a wire, the output end of the temperature sensor 21 is electrically connected to the input end of the single-chip microcomputer 18 through a wire, and the output end of the single-chip microcomputer 18 is electrically connected to the first air pump 11, the electric heating wire 12 and the input end of the second air pump 16 through a wire.

[0022] In this implementation:

[0023] Furthermore, culture dish placement grooves 10 are evenly arranged on the top of the culture plate 5, and culture dishes 7 are installed inside the culture dish placement grooves 10. Multiple groups of culture dishes 7 for culturing biological cells can be placed inside the culture dish placement grooves 10 on the culture plate 5 respectively.

[0024] Furthermore, connecting tubes 22 are evenly installed on the outer side of the mounting column 19, and the connecting tubes 22 are all connected to the heating chamber 20, and the connecting tubes 22 all pass through the culture plate 5 and extend to the interior of the heating groove 8. The heated gas enters the interior of the heating chamber 20 through the heating tube 13, and is diverted to the interior of the heating groove 8 on the multiple groups of culture plates 5 through multiple groups of connecting tubes 22. The heat can be evenly transferred to different culture dishes 7 through the culture plates 5, which is beneficial to keep the temperature of the culture dishes 7 uniform.

[0025] Furthermore, a humidifying tube 2 is installed at one end of the humidifying water tank 14 away from the second vacuum pump 16, and the humidifying tube 2 passes through the incubator 1 and extends to the interior of the incubator 1. The humidified gas inside the humidifying water tank 14 is blown back into the interior of the incubator 1 through the humidifying tube 2, thereby increasing the humidity inside the incubator 1.

[0026] Furthermore, an outlet pipe 15 extending to the interior of the humidification water tank 14 is installed at the output end of the second air pump 16, and an air guide pipe 17 is installed at the input end of the second air pump 16. The air guide pipe 17 passes through the mounting plate 4. The second air pump 16 extracts the gas inside the incubator 1 through the air guide pipe 17, and blows the gas into the interior of the humidification water tank 14 through the outlet pipe 15.

[0027] Furthermore, an air duct 9 is provided at the top of the incubator 1 away from the temperature sensor 21, and a dustproof net is installed inside the air duct 9. The gas blown out from the heating tank 8 can be discharged from the inside of the incubator 1 through the air duct 9, so that the gas inside the incubator 1 is kept flowing.

[0028] Furthermore, a door 23 is installed on one side of the incubator 1 , and a handle is installed on the outer side of the door 23 to facilitate taking and placing the culture dish 7 .

[0029] Working principle:

[0030] When in use, the device is powered on, a proper amount of distilled water is added into the humidification water tank 14, and then the door 23 is closed. The staff can place multiple groups of culture dishes 7 for culturing biological cells in the culture dish placement slots 10 on the culture plate 5 respectively;

[0031] At this time, the humidity sensor 6, the single-chip microcomputer 18 and the temperature sensor 21 are powered on and work, and the humidity sensor 6 monitors the temperature inside the incubator 1 in real time and transmits the monitored information to the single-chip microcomputer 18;

[0032] When the temperature inside the incubator 1 is lower than the threshold value preset by the single chip microcomputer 18, the single chip microcomputer 18 controls the first vacuum pump 11 and the electric heating wire 12 to start, and the first vacuum pump 11 draws gas into the heating tube 13, and the gas is heated by the electric heating wire 12. The heated gas enters the heating chamber 20 through the heating tube 13, and is diverted to the heating slots 8 on the multiple groups of culture plates 5 through the multiple groups of connecting tubes 22, so that the heat can be evenly transferred to different culture dishes 7 through the culture plates 5, which is conducive to keeping the temperature of the culture dishes 7 uniform;

[0033] When the temperature inside the incubator 1 is higher than the threshold value preset by the single chip microcomputer 18, the single chip microcomputer 18 controls the first vacuum pump 11 and the electric heating wire 12 to turn off, and the heating process is stopped. The single chip microcomputer 18 controls the temperature inside the incubator 1 to be kept within a constant range.

[0034] At the same time, the temperature sensor 21 monitors the temperature inside the incubator 1 in real time, and transmits the monitored information to the single chip microcomputer 18. When the humidity inside the incubator 1 is lower than the threshold value preset by the single chip microcomputer 18, the single chip microcomputer 18 controls the second vacuum pump 16 to start, and the second vacuum pump 16 draws the gas inside the incubator 1 into the humidification water tank 14, and can humidify the gas with the water inside the humidification water tank 14, and blow the gas back into the incubator 1 through the humidification pipe 2, so as to increase the humidity inside the incubator 1;

[0035] When the humidity inside the incubator 1 is higher than the threshold value preset by the single chip microcomputer 18, the single chip microcomputer 18 controls the second vacuum pump 16 to turn off and stop the heating process. The single chip microcomputer 18 controls the humidity inside the incubator 1 to be kept within a constant range.

[0036] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-restrictive from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention. Any reference numeral in a claim should not be regarded as limiting the claim to which it relates.

Claims

1. A cell culture box for biological cell research, comprising a culture box (1), a mounting plate (4), a culture plate (5) and a mounting column (19), characterized in that: The incubator (1) is provided with a mounting plate (4) inside, and a mounting column (19) is installed at the middle position of the top of the mounting plate (4). A heating chamber (20) is arranged inside the mounting column (19), and culture plates (5) are evenly installed on the outside of the mounting column (19). The ends of the culture plates (5) away from the mounting column (19) are all provided with heating grooves (8). A first vacuum pump (11) is installed at one end of the bottom of the mounting plate (4). An vacuum pipe (3) extending to the outside of the incubator (1) is installed at the input end of the first vacuum pump (11), and a heating pipe (13) is installed at the output end of the first vacuum pump (11). The heating pipe (13) passes through the mounting plate (4) and extends to the inside of the heating chamber (20), and an electric heating wire (12) is wound around the outside of the heating pipe (13). The bottom of the incubator (1) is away from the first vacuum pump (11). A humidifying water tank (14) is installed at one end of the vacuum pump (11); a second vacuum pump (16) is installed at the end of the bottom of the mounting plate (4) away from the first vacuum pump (11); a humidity sensor (6) is installed at the top of the incubator (1) near the end of the first vacuum pump (11); and a temperature sensor (21) is installed at the top of the incubator (1) away from the humidity sensor (6); a single-chip computer (18) is installed at the top of the incubator (1); an output end of the humidity sensor (6) is electrically connected to an input end of the single-chip computer (18) through a wire; an output end of the temperature sensor (21) is electrically connected to an input end of the single-chip computer (18) through a wire; and an output end of the single-chip computer (18) is electrically connected to the input ends of the first vacuum pump (11), the electric heating wire (12) and the second vacuum pump (16) through wires.

2. A cell culture incubator for biological cell research according to claim 1, characterized in that: The top of the culture plate (5) is evenly provided with culture dish placement grooves (10), and the inside of the culture dish placement grooves (10) is installed with culture dishes (7).

3. A cell culture incubator for biological cell research according to claim 1, characterized in that: The outer sides of the mounting columns (19) are evenly mounted with connecting tubes (22), and the connecting tubes (22) are all connected to the heating chamber (20), and the connecting tubes (22) pass through the culture plate (5) and extend to the interior of the heating tank (8).

4. A cell culture incubator for biological cell research according to claim 1, characterized in that: A humidifying tube (2) is installed at one end of the humidifying water tank (14) away from the second vacuum pump (16), and the humidifying tube (2) passes through the incubator (1) and extends to the interior of the incubator (1).

5. The cell culture incubator for biological cell research according to claim 1, characterized in that: The output end of the second air pump (16) is equipped with an air outlet pipe (15) extending to the interior of the humidification water tank (14), and the input end of the second air pump (16) is equipped with an air guide pipe (17), and the air guide pipe (17) passes through the mounting plate (4).

6. A cell culture incubator for biological cell research according to claim 1, characterized in that: An air guide port (9) is provided at the top of the incubator (1) at one end away from the temperature sensor (21), and a dustproof net is installed inside the air guide port (9).

7. A cell culture incubator for biological cell research according to claim 1, characterized in that: A door (23) is installed on one side of the incubator (1), and a handle is installed on the outer side of the door (23).

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