Full-automatic chromosome karyotype analysis glass slide array drop sample preparation equipment

Abstract

The utility model discloses full-automatic chromosome karyotype analysis glass slide array drop sample preparation equipment which comprises a workbench, a position moving system, an electromagnetic valve, a high-pressure pump and a liquid dropping gun, the liquid dropping gun is installed on the position moving system and connected with a liquid storage device, the liquid dropping gun is connected with the electromagnetic valve, the electromagnetic valve is connected to the high-pressure pump through a guide pipe, and the high-pressure pump is connected with the liquid storage device. The high-pressure pump is connected with the liquid storage device through another guide pipe, and an intermittent liquid injection control structure for the liquid dropping gun is formed through cooperation of the electromagnetic valve and the high-pressure pump. Therefore, the volume of each time of sample dropping can be controlled within the range of 0.01 ul to 0.1 ul, the liquid dropping gun is controlled by the position moving system to move above the glass slide at a high-precision stepping interval and repeat sample dropping, and a high-resolution liquid dropping array can be dropped on the glass slide. Therefore, the chromosome drop sample on the glass slide has better repeatability and reproducibility without depending on the dispersion process of the drop liquid on the glass.

Description

Technical Field

[0001] The utility model relates to the technical field of micro-sample preparation, in particular to a glass slide preparation device for cell chromosome karyotype analysis. Background Art

[0002] The karyotype analysis of cell chromosomes is an important means of modern medical genetics research and clinical testing. By dropping the slide, the cell chromosomes are evenly and clearly displayed on the slide, so that people can observe and identify the differences in chromosome karyotypes through a microscope, and the preparation of slides for cell chromosome karyotype analysis is the basis for realizing this technology. The preparation of slides for cell chromosome karyotype analysis is generally carried out in a controllable constant temperature and humidity environment, such as a temperature of 10-50 degrees and a humidity of 10%-90%. It usually includes the following steps: first, the slide is arranged, then a fixative is dripped onto the slide, and then the sample is dripped onto the fixative, and the preparation is completed when the sample is evenly dispersed. The applicant has filed a Chinese utility model patent application entitled "A fully automatic cell chromosome karyotype analysis slide preparation device", which uses a stepping motor to control a sample drop syringe (liquid drop gun) to drop a sample in the range of 5ul-30ul each time, and the sample is dripped onto a glass slide and then slowly dispersed onto the entire glass. The disadvantage of this method is that the dispersion speed and dispersion effect of the drop sample will be affected by factors such as the cleanliness of the glass sheet, the effect of atomization pretreatment, the temperature and humidity of the air, etc. The repeatability and consistency of the dispersion are difficult to control. At the same time, the waiting time for decomposition is long, which affects the preparation efficiency. Utility Model Content

[0003] In view of the shortcomings of the prior art, the utility model provides a fully automatic chromosome karyotype analysis slide array sample preparation device with a simple structure, more reasonable design, higher preparation efficiency, faster sample dispersion speed, higher uniformity, and better dispersion repeatability and consistency.

[0004] In order to solve the above technical problems, the utility model adopts the following technical scheme: a fully automatic chromosome karyotype analysis slide array sample preparation device, including a workbench, a position movement system and a dropper gun, the position movement system is installed on the workbench, the slide is placed on the workbench through a slide rack, the dropper gun is installed on the position movement system and is connected to a liquid reservoir, and is characterized in that: it also includes a solenoid valve and a high-pressure pump, the dropper gun is connected to the solenoid valve, the solenoid valve is connected to the high-pressure pump through a conduit, the high-pressure pump is connected to the liquid reservoir through another conduit, and an intermittent liquid injection control structure for the dropper gun is formed through the cooperation of the solenoid valve and the high-pressure pump.

[0005] Furthermore, the position movement system includes an X-axis mechanism, a Y-axis mechanism and a moving mechanism, the Y-axis mechanism is installed on the workbench, the X-axis mechanism is movable across the Y-axis mechanism, the moving mechanism is installed on the X-axis mechanism, and the dropper gun and the solenoid valve are installed together on the moving mechanism so as to achieve X, Y, and Z three-dimensional movement.

[0006] A multi-way valve can be used as a switching device between the high-pressure pump, the solenoid valve and the liquid reservoir.

[0007] Preferably, the solenoid valve adopts a solenoid valve with a minimum switching gap of 20ms, and the model is not limited.

[0008] Preferably, the high-pressure pump is a syringe pump, a plunger pump or a double diaphragm pump, etc., which can exert a maximum pressure of 100 PSI, and the model is not limited.

[0009] Preferably, the amount of liquid dripped out by the dripping gun each time is 0.01ul-0.1ul.

[0010] Preferably, the distance that the dripping gun moves each time is 0.3-0.6 mm and is adjustable.

[0011] The utility model replaces the original pump by providing pressure with a solenoid valve to control the flow rate, so that the volume of each drop can be controlled within the range of 0.01ul-0.1ul, and the stepping motor of the X-axis mechanism and the Y-axis mechanism controls the dropper to move and repeat the drop at a high-precision (such as 0.5mm) step interval above the slide glass, so that a high-resolution (such as 0.5mm interval) drop array can be dropped on the slide glass. In this way, the drop is very uniform, so there is no need to rely on the dispersion process of the drop on the glass, so that the chromosome drop sample on the slide glass has better repeatability and reproducibility. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 It is a schematic diagram of the structure of the utility model;

[0013] Figure 2 Schematic diagram of the glass slide after the droplets are deposited.

[0014] In the figure, 1 is a workbench, 11 is an X-axis mechanism, 12 is a Y-axis mechanism, 2 is a moving mechanism, 3 is a dropper gun, 4 is an electromagnetic valve, 5 is a high-pressure pump, 6 is a liquid reservoir, 7 is a slide glass, and 8 is a droplet. DETAILED DESCRIPTION

[0015] In this embodiment, refer to Figure 1 and Figure 2The fully automatic chromosome karyotype analysis slide array sample drop preparation device includes a workbench 1, a position movement system and a dropper gun 3, the position movement system is installed on the workbench 1, the slide 7 is placed on the workbench 1 through a slide rack, the dropper gun 3 is installed on the position movement system and is connected to a liquid reservoir 7; it also includes a solenoid valve 4 and a high-pressure pump 5, the dropper gun 3 is connected to the solenoid valve 4, the solenoid valve 4 is connected to the high-pressure pump 5 through a catheter, and the high-pressure pump 5 is connected to the liquid reservoir 6 through another catheter. The cooperation of the solenoid valve 4 and the high-pressure pump 5 forms an intermittent liquid injection control structure for the dropper gun, so that the flow rate can be accurately controlled so that the volume of each dropper sample is within the range of 0.01ul-0.1ul.

[0016] The position moving system includes an X-axis mechanism 11, a Y-axis mechanism 12 and a moving mechanism 2, wherein the Y-axis mechanism 12 is mounted on the workbench 1, the X-axis mechanism 11 is movable across the Y-axis mechanism 12, the moving mechanism 2 is mounted on the X-axis mechanism 11, and the dropper gun 3 and the solenoid valve 4 are mounted together on the moving mechanism 2, so as to realize X, Y, and Z three-dimensional movement. This structure belongs to the prior art and will not be described in detail here.

[0017] The solenoid valve 4 uses a solenoid valve with a minimum switching gap of 20ms. By controlling the switching time gap of the solenoid valve 4, the liquid taking / injection amount of the dripping gun 3 is controlled. When the solenoid valve 4 is in an open state, the water pressure on the solenoid valve 4 is transmitted to the dripping gun 3 below, and when the solenoid valve 4 is in a closed state, the water pressure is isolated.

[0018] The high-pressure pump 5 adopts a syringe pump (or a plunger pump, a double diaphragm pump, etc.) that can apply a maximum pressure of 100 PSI. It cooperates with a pressure sensor (built-in) to enable the water in the catheter to provide continuous pressure to the solenoid valve 4.

[0019] The amount of liquid dropped by the dropper 3 each time is 0.01ul-0.1ul, and the distance moved by the dropper 3 each time is 0.5mm. In this way, an array of several droppers 8 with a resolution of 0.5mm and uniform intervals can be obtained on the slide glass 7.

[0020] The present invention has been described in detail above. The above description is only a preferred embodiment of the present invention and should not limit the scope of implementation of the present invention. That is, all equivalent changes and modifications made within the scope of this application should still fall within the scope of the present invention.

Claims

1. A fully automatic chromosome karyotype analysis slide array sample preparation device, comprising a workbench, a position moving system and a dropper, wherein the position moving system is mounted on the workbench, and the dropper is mounted on the position moving system and connected to a liquid reservoir, characterized in that: It also includes a solenoid valve and a high-pressure pump. The dripping gun is connected to the solenoid valve. The solenoid valve is connected to the high-pressure pump through a conduit. The high-pressure pump is connected to the liquid reservoir through another conduit. The cooperation of the solenoid valve and the high-pressure pump forms an intermittent liquid injection control structure for the dripping gun.

2. The fully automatic chromosome karyotype analysis slide array sample preparation device according to claim 1, characterized in that: The position moving system includes an X-axis mechanism, a Y-axis mechanism and a moving mechanism. The Y-axis mechanism is installed on a workbench, the X-axis mechanism movable span is arranged on the Y-axis mechanism, the moving mechanism is installed on the X-axis mechanism, and the dropper gun and the solenoid valve are installed together on the moving mechanism to form a structure capable of X, Y and Z three-dimensional movement.

3. The fully automatic chromosome karyotype analysis slide array sample preparation device according to claim 1, characterized in that: The solenoid valve adopts a solenoid valve with a minimum switching gap of 20ms.

4. The fully automatic chromosome karyotype analysis slide array sample preparation device according to claim 1, characterized in that: The high-pressure pump adopts a syringe pump, a plunger pump or a double diaphragm pump which can apply a maximum pressure of 100 PSI.

5. The fully automatic chromosome karyotype analysis slide array sample preparation device according to claim 1, characterized in that: The amount of liquid dropped by the dropper gun each time is 0.01ul-0.1ul.

6. The fully automatic chromosome karyotype analysis slide array sample preparation device according to claim 1, characterized in that: The distance that the dripping gun moves each time is 0.3-0.6 mm.

Images