Culture apparatus, culture container, and cover assembly

Abstract

The utility model provides culture equipment, a culture container and a cover body assembly. The culture equipment comprises a culture container, the culture container comprises a container body and a cover body for sealing the container body, and the cover body is at least provided with a culture solution pipe and a liquid passing pipe; the culture solution part at least comprises a culture solution container and is communicated with the culture container through a culture solution pipe; the collection container is communicated with the culture container through the liquid passing pipe; the pump unit is communicated with the culture container, the culture solution part and the collection container and is used for pumping the culture solution, the washing solution or the waste solution; the movement mechanism is connected to the culture container, the culture container can be located at a culture position or a liquid changing position, and the culture container can be switched between the culture position or the liquid changing position through the movement mechanism.

Description

Technical Field

[0001] The present application relates to a culture device, a culture container and a cover assembly. Background Art

[0002] When culturing adherent cells, it is usually necessary to regularly replace the culture medium to provide the cells with the nutrients they need for growth. The traditional way to replace the culture medium is for the operator to manually discard the old culture medium and add new culture medium using a pipette, but manual liquid replacement consumes a lot of manpower and time in large-scale culture. In addition, in life science research, it is also necessary to provide cells with culture medium with different components. For example, provide cells with culture medium containing different rhythmic factors at different rhythmic times to simulate biological rhythm regulation in vitro; replace the culture medium with a culture medium containing specific antibiotics or drugs during cell screening. In these cases, the culture medium needs to be replaced frequently, resulting in heavy labor costs.

[0003] Existing automatic culture medium replacement equipment can only replace a single culture medium, and cannot switch, clean and automatically replace multiple component culture media; in addition, the existing automatic liquid replacement device needs to connect the culture container to the outside when changing the liquid, increasing the risk of contamination of the culture object. Utility Model Content

[0004] The purpose of the present application is to achieve efficient replacement of culture medium during biological culture and reduce the risk of contamination of cultured objects.

[0005] A culture device according to the first aspect of the present application includes a culture container, including a container body and a cover body that seals the container body, the cover body being provided with at least a culture liquid tube and a liquid passage tube; a culture liquid portion, including at least a culture liquid container, being connected to the culture container through the culture liquid tube; a collecting container, being connected to the culture container through the liquid passage tube; a pump unit, being connected to the culture container, the culture liquid portion and the collecting container, and being used for pumping culture liquid, washing liquid or waste liquid; and a motion mechanism, connected to the culture container, the culture container being capable of being located at a culture position or a liquid exchange position, and the culture container being switched between the culture position and the liquid exchange position through the motion mechanism.

[0006] In one or more embodiments of the culture device, the culture liquid part includes a first culture liquid container and a second culture liquid container, which are respectively connected to the culture container; preferably, the cover body includes a first culture liquid tube and a second culture liquid tube, the first culture liquid container of the culture liquid part is connected to the culture container through the first culture liquid tube, and the second culture liquid container of the culture liquid part is connected to the culture container through the second culture liquid tube; more preferably, the cover body is also provided with a vent hole, and the vent hole is connected to a filtering device or is sealed by a waterproof and breathable membrane.

[0007] In one or more embodiments of the culture device, the motion mechanism includes a rotating mechanism; preferably, the motion mechanism also includes a moving mechanism; more preferably, the motion mechanism also includes a position sensing device for detecting the culture container located at the culture position, which is a horizontal position.

[0008] In one or more embodiments of the culture device, the culture container has a washing stroke within an angle range of ±α, and the culture container can be driven by the motion mechanism to reciprocate within an angle range of ±α; preferably, α is selected from any angle between 1° and 20°.

[0009] In one or more embodiments of the culture device, the angle A between the bottom edge of the culture container and the rotation axis when flipped is 30° to 60°; preferably, the angle B between the cell adherent culture surface and the horizontal plane in the fluid replacement position is 100° to 150°.

[0010] In one or more embodiments of the culture device, the parts of the culture fluid tube and the liquid passage tube extending into the culture container have a bending section, and at the liquid exchange position, the ends of the culture fluid tube and the liquid passage tube are located at the bottom of the culture container; preferably, the ends of the culture fluid tube and the liquid passage tube are covered with a silicone sleeve.

[0011] In one or more embodiments of the culture device, the collection container includes a washing liquid container and a waste liquid container, and the washing liquid container and the waste liquid container are connected to the culture container through the liquid passage tube.

[0012] In one or more embodiments of the culture apparatus, the culture container, the culture liquid portion, the collection container, and the pump unit are supported by a well plate.

[0013] According to the second aspect of the present application, a cover assembly is used for the culture device as described in the first aspect, the cover assembly includes a cover, and a pipeline arranged on the cover, the pipeline includes at least a culture liquid tube and a liquid passage tube, and the cover seals the container body.

[0014] A culture container according to the third aspect of the present application is used for the culture device as described in the first aspect, comprising a container body and a cover body sealing the container body, wherein the cover body is provided with at least a culture liquid tube and a liquid passage tube.

[0015] According to a fourth aspect of the present application, a culture method using the culture device described in the first aspect comprises the following steps:

[0016] S1. The pump unit pumps the first culture fluid of the first culture fluid container into the culture container through the first culture fluid tube; or directly inoculates adherent cells in the culture container;

[0017] S2. Maintain the first rhythmic culture period;

[0018] S3. The pump unit pumps the first culture solution of the culture container into the first culture solution container through the first culture solution tube;

[0019] S4. The pump unit pumps the wash solution through the liquid pipe into the culture container;

[0020] S5. The pump unit pumps the wash solution from the culture container to the collection container through the liquid pipe;

[0021] S6. The pump unit pumps the second culture solution of the second culture solution container into the culture container through the second culture solution tube;

[0022] S7. Maintain the second rhythmic culture period.

[0023] In one or more embodiments of the culture method, after S4 and before S5, the culture container is driven by a motion mechanism to reciprocate and rotate within an angle range of ±α for washing.

[0024] In one or more embodiments of the culture method, the α is selected from any angle between 1° and 20°; the rotation speed is 5 to 40 revolutions per minute; preferably, the α is selected from any angle between 3° and 8°; the rotation speed is 5 to 20 revolutions per minute.

[0025] In one or more embodiments of the culture method, during the first rhythmic culture time period and the second rhythmic culture time period, the culture container is located at a culture position.

[0026] In one or more embodiments of the culture method, after S2 and before S3, the culture container is driven by the motion mechanism to switch from the culture position to the liquid replacement position; after S6 and before S7, the culture container is driven by the motion mechanism to switch from the liquid replacement position to the culture position.

[0027] In one or more embodiments of the culture device, it includes: a memory for storing instructions executable by a processor; a processor for executing the instructions to implement the steps of the culture method of the fourth aspect that can be implemented by a computer program.

[0028] According to a fifth aspect of the present application, a computer-readable storage medium has a computer program thereon, and the program is executed by a processor to implement the steps in the cultivation method described in the fourth aspect that can be implemented by a computer program. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] The above and other features, properties and advantages of the present application will become more apparent through the following description in conjunction with the accompanying drawings and embodiments, in which:

[0030] Figure 1A , Figure 1B Schematic diagrams of the structure of a culture container of a culture device according to an embodiment in a culture position and a liquid replacement position respectively.

[0031] Figure 2 It is a schematic structural diagram of a culture liquid portion and a collection container of a culture device according to an embodiment.

[0032] Figure 3 It is a schematic diagram of the structure of a movement mechanism of a culture device and a culture container according to an embodiment.

[0033] Figure 4 It is a schematic diagram of the structure of a culture container according to an embodiment.

[0034] Figure 5 It is a schematic structural diagram of a cover assembly of an embodiment.

[0035] Figure 6 It is a schematic diagram of a culture method according to an embodiment. DETAILED DESCRIPTION

[0036] The following discloses a variety of different implementation methods or embodiments of the subject technical solution. To simplify the disclosure, specific examples of each element and arrangement are described below. Of course, these are only examples and are not intended to limit the scope of protection of the present invention. For example, the first feature subsequently recorded in the specification is formed above or on the second feature, which may include an implementation method in which the first and second features are formed by direct connection, and may also include an implementation method in which an additional feature is formed between the first and second features, so that the first and second features may not be directly connected. In addition, the figure marks and / or letters may be repeated in different examples in these disclosures. This repetition is for simplicity and clarity, and does not itself represent the relationship between the various implementation methods and / or structures to be discussed. Further, when the first element is described in a manner connected or combined with the second element, the description includes an implementation method in which the first and second elements are directly connected or combined with each other, and also includes the use of one or more other intervening elements to indirectly connect or combine the first and second elements.

[0037] It is to be understood that the following uses a flow chart to illustrate the steps performed by the culture method according to the embodiments of the present application. It should be understood that, according to actual circumstances, the preceding or following steps may not necessarily be performed accurately in order. Other steps may also be added to these processes, or a step or several steps may be removed from these processes.

[0038] refer to Figure 1A , Figures 1B to 4 As shown, the culture device 100 includes a culture container 1 , a culture liquid portion 2 , a collection container 3 , and a pump unit 4 .

[0039] like Figure 1A as well as Figure 1B As shown, in some embodiments, the culture container 1, the culture liquid part 2, the collection container 3, and the pump unit 4 can be supported on the orifice plate 8. The orifice plate 8 can be arranged in the incubator, and a humidity source, such as water, can be arranged below the orifice plate 8. The porous design of the orifice plate 8 is conducive to gas exchange, reducing the influence of the culture device on the gas circulation, so that the gas inside the culture device 100 can be uniform.

[0040] like Figure 4 As shown, the culture container 1 may include a container body 11 and a cover body 12 that seals the container body 11. The cover body 12 is provided with at least a culture liquid tube and a liquid passage 183. The culture liquid tube may include a first culture liquid tube 181 as shown in the figure, and may also include a second culture liquid tube 182. It can be understood that the material of the culture liquid tube and the liquid passage may be stainless steel, or plastic, silicone or latex, and is not limited thereto. The first culture liquid tube 181 and the second culture liquid tube 182 may be specifically used to transport the first culture liquid and the second culture liquid. The liquid passage 183 may transport washing liquid or waste liquid. It can be understood that the number of culture liquid tubes is not limited to the first culture liquid tube and the second culture liquid tube shown in the figure. For example, if the culture involves only one culture liquid, only the first culture liquid tube 181 is required. If the culture requires three or more culture liquids, then correspondingly, on the basis of the first culture liquid tube 181 and the second culture liquid tube 182, a third culture liquid tube specifically transporting the third culture liquid and a fourth culture liquid tube for the fourth culture liquid need to be provided, and so on. However, it can be understood that the culture fluid tube can also be used to transport a single type of culture fluid. For example, the first culture fluid tube 181 can transport the second culture fluid in addition to the first culture fluid. Such a structure is more simplified. Figure 1A , Figure 1B as well as Figure 4 As shown, the cover body may also be provided with a vent 184, and the vent 184 may be connected to a filter device (such as an air filter) through an air pipe. However, this is not limiting, for example, it is also possible that no air pipe is required, but as Figure 5The waterproof breathable membrane is shown to seal the vent 184. The sealing method of the culture container 1 can be that the cover body 12 is sealed with a gasket or a sealing plug 17, and the material of the gasket and the sealing plug can be rubber or silicone, but it is not limited to this. The container body 11 of the culture container can be a standard T25 cell culture flask, a T75 cell culture flask, etc., but it is not limited to this. Taking the culture object as cells as an example, before culturing, the culture container 1 is a culture container inoculated with cells, and the culture container 1, the culture liquid part 2 and the collection container 3 of the culture equipment are sterilized before use, such as irradiation sterilization, ultraviolet sterilization, moist heat sterilization, etc., and the pipelines are connected in a sterile environment.

[0041] like Figure 1A , Figure 1B as well as Figure 2 As shown, the culture liquid part 2 at least includes a first culture liquid container 6, which is connected to the culture container 1 through a first culture liquid tube 181; and may also include a second culture liquid container 7, which is connected to the culture container 1 through a second culture liquid tube 182. The meaning of the first culture liquid container 6 and the second culture liquid container 7 is that they are specifically used to contain the first culture liquid and the second culture liquid, respectively, that is, the first culture liquid container 6 for containing the first culture liquid and / or the second culture liquid container 7 for containing the second culture liquid. However, it can be understood that a container can also contain multiple culture liquids, for example, the same container contains the first culture liquid and the second culture liquid at the same time, but a corresponding partition needs to be set in the same container to separate the first culture liquid and the second culture liquid to prevent the two from mixing; or in the same container, after the first culture liquid is pumped away, the second culture liquid is pumped in, so as to circulate, in short, it is necessary to avoid mixing the first culture liquid and the second culture liquid. The first culture liquid container and / or the second culture liquid container are sealed by a culture liquid cover body, and the sealing method may be that a gasket or a sealing plug is provided on the culture liquid cover body, and the material of the gasket and the sealing plug may be rubber or silicone, but is not limited thereto. Preferably, it is sealed by a sealing plug 22. The culture liquid cover body is provided with an inlet and outlet liquid pipeline 23 and an air vent, and the air vent may be connected to a filter device (such as an air filter) through an air pipe, or an air pipe is not required, but a waterproof breathable membrane is provided to seal the air vent. Preferably, the air vent is connected to a filter device through an air pipe. The inlet and outlet liquid pipelines are connected to the pump, and the material of the connecting pipeline includes but is not limited to a stainless steel pipe, a plastic pipe, a silicone tube, a latex tube, and the pipeline connection method is such as Figure 1A , Figure 1B It can be understood that, in some embodiments, the structure of the culture liquid cover of the culture liquid portion 2 can be similar to the structure of the cover 18 of the culture container 1 .

[0042] The collection container 3 is connected to the culture container 1 through the liquid pipe 183. Figure 2As shown, the culture liquid part 2 and the collection container 3 can be arranged adjacent to each other and supported by the same bracket. The specific form of the collection container 3 can be, for example, a glass test tube, a reagent bottle, or a glass bottle. Preferably, the collection container 3 can include a washing liquid container 32 for storing washing liquid in addition to a waste liquid container 31 for collecting waste liquid, and both can be connected to a one-way valve 33 and a filter 34 respectively, and the two one-way valves are in opposite directions and are connected by a T-type connector 35.

[0043] The pump unit 4 is connected to the culture container 1, the culture liquid part 2, and the collection container 3, and is used to pump the culture liquid, the washing liquid, and the waste liquid. Figure 1A , Figure 1B As shown, the pump unit 4 generally includes a plurality of pumps, and the type of the pump may be a peristaltic pump, a gear pump, a diaphragm pump, a syringe pump, and the like.

[0044] It can be understood that the culture fluid container and culture fluid tube included in the culture fluid section 2 are not limited to the two shown in the figure, that is, they are not limited to the first culture fluid container, the second culture fluid container, and the first culture fluid tube and the second culture fluid tube. Here, only the circadian rhythm culture is taken as an example. Therefore, it is only necessary to set the first culture fluid tube 181, the second culture fluid tube 182, the first culture fluid container 6, and the second culture fluid container 7 corresponding to the culture time periods during the day and night. If a more complex rhythm is required, for example, including three or more rhythmic culture time periods, a corresponding third culture fluid tube, a fourth culture fluid tube, a third culture fluid container, a fourth culture fluid container, etc. can be set. Figure 2 As shown, the culture fluid section 2 may include a plurality of culture fluid containers, and the number of culture fluid containers and culture fluid tubes may be increased or decreased according to specific rhythmic culture requirements. For example, in an extreme case, there is only one rhythmic time period, which is normal culture. In this case, generally only one culture container and one culture fluid tube are required.

[0045] refer to Figure 1A , Figure 1B as well as Figure 3 As shown, the culture device 100 also includes a motion mechanism 5 connected to the culture container 1. The culture container 1 can be located at a culture position 101 or a liquid replacement position 102. The culture container 1 is switched between the culture position 101 and the liquid replacement position 102 by the motion mechanism 5. The culture position 101 here is the position of the culture container 1 when cell culture is performed; and the liquid replacement position 102 is the position of the culture container 1 when switching or replacing the culture solution, washing solution, and waste solution between different rhythmic time periods. Preferably, as Figure 3As shown, the culture position 101 can be a horizontal position, that is, whether it is the first rhythmic culture time period or the second rhythmic culture time period, it is kept in a horizontal position, and such a culture effect is better. Preferably, in some embodiments, the culture device 100 can also be provided with a position sensing device, such as a tilt sensor 22, to sense whether the position of the culture container 1 remains horizontal during the culture time period. If a slight deviation occurs, the motion mechanism 5 can also be fine-tuned to keep the culture container 1 in a horizontal position.

[0046] When liquid replacement is required, the culture container 1 is first switched from the culture position 101 to the liquid replacement position 102 by the motion mechanism 5 . After the liquid replacement is completed, the culture container 1 is then switched from the liquid replacement position 102 to the culture position 101 by the motion mechanism 5 .

[0047] like Figure 3 As shown, the specific structure of the motion mechanism 5 may include a rotating mechanism 15 and a moving mechanism 20. The rotating mechanism 15 may be, for example, a stepping motor with a brake and an encoder, and its output is rotated to the first plate 19, on which the culture container 1 is arranged. Preferably, as Figure 1A As shown, the angle A between the bottom edge of the culture container 1 and the edge of the first plate 19 can be 30° to 60°, that is, the angle A between the bottom edge of the culture container 1 and the rotation axis when flipping is 30° to 60°, preferably, the angle A can be 40° to 50°, more preferably, the angle A can be 45°, so that the liquid level in the container is the highest when the culture container 1 is switched to the liquid replacement position 102, which is convenient for liquid suction. The moving mechanism 20 can be, for example, a linear motor module as shown in the figure, which is fixedly connected to the second plate 21. The moving mechanism 20 and the second plate 21 can be fixedly connected by a silicone fixing sheet to play a buffering role. Figure 3 The rotating mechanism 15 shown can rotate the culture container 1 to a certain angle, and the moving mechanism 20 can move the culture container 1 in a vertical direction. It can be understood that the use of the moving mechanism 5 can make the culture device easier to perform the liquid replacement process and keep the position of the culture container 1 horizontal during the culture period.

[0048] Specifically, the liquid replacement process may be that the culture container 1 is driven by the rotating mechanism 15 to Figure 1A The horizontal culture position 101 in the Figure 1BThe cell-adherent culture surface is at an angle B of about 100° to 150° with the horizontal plane at the medium-changing position 102 shown. Preferably, the angle B is about 120° to 140°, and more preferably, the angle B is about 135°. The angle A between the bottom edge of the culture container 1 and the rotation axis during flipping is a fixed angle of 30° to 60°, and the angle B between the cell-adherent culture surface and the horizontal plane is a flipping angle of 100° to 150°, so that the original culture medium in the culture container 1 is gathered at the bottom of the bottle and has the deepest depth under the same volume. Preferably, as Figure 4 As shown, the first culture fluid tube 181, the second culture fluid tube 182, and the liquid tube 183 extending into the culture container 1 have a bent section 180, so that when at the liquid replacement position 102, the ends of the first culture fluid tube 181, the second culture fluid tube 182, and the liquid tube 183 are located at the bottom of the culture container 1, that is, they extend all the way to the vertex opposite to the cell adherent culture surface in the culture container 1 to ensure maximum liquid absorption, preferably, the ends are also covered with a silicone sleeve to increase the contact between the tube wall and the bottle wall. The peristaltic pump of the pump unit 4 is turned on to pump the culture fluid in the culture container 1 back to the culture fluid container for the corresponding culture fluid.

[0049] During the liquid replacement process, after the culture liquid (e.g., the first culture liquid) of the previous culture time period is drawn off, the culture container 1 can be washed to avoid the influence of the residual culture liquid of different components on the culture effect. The pump unit is turned on, and the washing liquid in the washing liquid container is injected into the culture container 1. The culture container 1 is driven by the rotating mechanism 15 to return to the horizontal state in the reverse direction, and reciprocates within the angle range of ±α. α can be selected from any angle between 1° and 20°, and the oscillation speed can be 5 to 40 turns per minute, so that it can oscillate near the horizontal position to achieve a better washing effect. Preferably, α can be selected from any angle between 3° and 8°, and the oscillation speed is 5 to 20 turns per minute. Then the culture container 1 is rotated to the liquid replacement position 102, so that the waste liquid after washing is extracted into the waste liquid container, and the culture liquid (e.g., the second culture liquid) of the next culture time period is injected, and then it is flipped to the culture position 101 for the next rhythmic culture time period. It can be understood that the parameters such as the frequency, number of automatic liquid replacement, liquid replacement amount, flip angle and flip speed can be set by the user.

[0050] In summary, the culture device 100 introduced in the above embodiment has the beneficial effect that it can realize automatic rhythmic culture, achieve high efficiency of rhythmic culture, and maintain a closed and sterile environment throughout the culture process, thereby avoiding contamination of the culture objects (such as cells) in the bottle when the waste liquid is discharged, and avoiding the influence of the residual culture medium of different components on the culture effect.

[0051] like Figure 6As shown, as described above, the present application further provides a culture method, using the culture device 100 described in the above embodiment, the culture method comprises the following steps:

[0052] S1. The pump unit pumps the first culture fluid of the first culture fluid container into the culture container through the first culture fluid tube; or the adherent cells may be directly inoculated in the culture container in the initial stage. At this time, since the first culture fluid has been input during the inoculation process, it is not necessary to pump the first culture fluid through the pump unit;

[0053] S2. Maintain the first rhythmic culture period;

[0054] S3. The pump unit pumps the first culture solution of the culture container into the first culture solution container through the first culture solution tube;

[0055] S4. The pump unit pumps the wash solution through the liquid pipe into the culture container;

[0056] S5. The pump unit pumps the wash solution from the culture container to the collection container through the liquid pipe;

[0057] S6. The pump unit pumps the second culture fluid of the second culture fluid container into the culture container through the second culture fluid tube;

[0058] S7. Maintain the second rhythmic culture period.

[0059] In one or more embodiments of the culture method, after S4 and before S5, the culture container is driven by the motion mechanism to reciprocate within an angle range of ±α for washing.

[0060] As described above, the angle range of α can be any angle selected from 1° to 20°; the rotation speed is 5 to 40 revolutions per minute; preferably, α can be selected from any angle between 3° and 8°, and the oscillation speed is 5 to 20 revolutions per minute.

[0061] In one or more embodiments of the culture method, during the first rhythmic culture time period and the second rhythmic culture time period, the culture container is located at a culture position.

[0062] In one or more embodiments of the culture method, after S2 and before S3, the culture container is driven by the motion mechanism to switch from the culture position to the liquid replacement position; after S6 and before S7, the culture container is driven by the motion mechanism to switch from the liquid replacement position to the culture position.

[0063] It can be understood that the above steps can be performed in a cycle, for example, the first rhythmic culture time period and the second rhythmic culture time period constitute a culture cycle, and multiple culture cycles are performed. It can be understood that the culture cycle can also include more rhythmic culture time periods, for example, a single culture cycle includes the first rhythmic culture time period, the second rhythmic culture time period, the third rhythmic culture time period, the fourth rhythmic culture time period, ..., the Nth rhythmic time period. Correspondingly, the process of replacing the third culture solution, the fourth culture solution, ..., the Nth culture solution is similar to the above processes S2 to S6, and will not be repeated here.

[0064] According to another aspect of the application, the present application also provides a computer-readable medium.

[0065] The computer-readable medium provided in the present application has computer instructions thereon. When the computer instructions are executed by a processor, the program can be executed by the processor to implement the steps executed by the program in the culture method described in the above embodiment.

[0066] In summary, the beneficial effects of the culture equipment, culture container, culture method and cover assembly introduced above are that they can achieve efficient replacement of culture medium during biological culture and reduce the risk of contamination of the culture object, especially they can achieve efficient rhythmic culture.

[0067] The steps of the method described in conjunction with the embodiments disclosed herein may be embodied directly in hardware, in a software module executed by a processor, or in a combination of the two. The software module may reside in a RAM memory, a flash memory, a ROM memory, an EPROM memory, an EEPROM memory, a register, a hard disk, a removable disk, a CD-ROM, or any other form of storage medium known in the art. An exemplary storage medium is coupled to a processor so that the processor can read and write information from / to the storage medium. In an alternative, a storage medium may be integrated into a processor. The processor and the storage medium may reside in an ASIC. The ASIC may reside in a user terminal. In an alternative, the processor and the storage medium may reside in a user terminal as discrete components.

[0068] In one or more exemplary embodiments, the functions described may be implemented in hardware, software, firmware, or any combination thereof. If implemented as a computer program product in software, each function may be stored on or transmitted by a computer-readable medium as one or more instructions or codes. Computer-readable media include both computer storage media and communication media, including any media that facilitates the transfer of a computer program from one place to another. Storage media may be any available media that can be accessed by a computer. As an example and not limitation, such a computer-readable medium may include RAM, ROM, EEPROM, CD-ROM or other optical disk storage, disk storage or other magnetic storage device, or any other medium that can be used to carry or store the desired program code in the form of an instruction or data structure and can be accessed by a computer. Any connection is also properly referred to as a computer-readable medium. For example, if the software is transmitted from a website, server, or other remote source using a coaxial cable, fiber optic cable, twisted pair, digital subscriber line (DSL), or wireless technologies such as infrared, radio, and microwaves, the coaxial cable, fiber optic cable, twisted pair, DSL, or wireless technologies such as infrared, radio, and microwaves are included in the definition of the medium. Disk and disc as used herein include compact disc (CD), laser disc, optical disc, digital versatile disc (DVD), floppy disk and Blu-ray disc, wherein disk often reproduces data magnetically, while disc reproduces data optically with lasers. Combinations of the above should also be included within the scope of computer-readable media.

[0069] Although the present application is disclosed as above with preferred embodiments, it is not intended to limit the present application, and any person skilled in the art may make possible changes and modifications without departing from the spirit and scope of the present application. Therefore, any modification, equivalent change and modification made to the above embodiments based on the technical essence of the present application without departing from the content of the technical solution of the present application shall fall within the scope of protection defined by the claims of the present application.

Claims

1. A culture device, characterized in that include: The culture container comprises a container body and a cover body sealing the container body, wherein the cover body is provided with at least a culture liquid tube and a liquid passage tube; A culture liquid part, comprising at least a culture liquid container, and connected to the culture container through a culture liquid tube; A collecting container, connected to the culture container through the liquid passage; A pump unit, connected to the culture container, the culture liquid portion, and the collection container, and used for pumping the culture liquid, the washing liquid, or the waste liquid; The motion mechanism is connected to the culture container, and the culture container can be located at a culture position or a liquid replacement position. The culture container is switched between the culture position and the liquid replacement position by the motion mechanism.

2. The culture device according to claim 1, characterized in that The culture liquid part includes a first culture liquid container and a second culture liquid container, which are respectively communicated with the culture container.

3. The culture device according to claim 2, characterized in that: The cover body includes a first culture liquid tube and a second culture liquid tube. The first culture liquid container of the culture liquid section is connected to the culture container through the first culture liquid tube, and the second culture liquid container of the culture liquid section is connected to the culture container through the second culture liquid tube.

4. The culture device according to claim 3, characterized in that: The cover body is also provided with a vent hole, and the vent hole is connected to a filtering device or is sealed by a waterproof and breathable membrane.

5. The culture device according to any one of claims 1 to 2, characterized in that: The motion mechanism includes a rotation mechanism.

6. The culture device according to claim 5, characterized in that The motion mechanism also includes a moving mechanism.

7. The culture device according to claim 5, characterized in that: The motion mechanism further comprises a position sensing device for detecting the culture container being located at the culture position, wherein the culture position is a horizontal position.

8. The culture device according to claim 2, characterized in that: The culture container has a washing stroke within an angle range of ±α, and the culture container can be driven by the motion mechanism to reciprocate within the angle range of ±α.

9. The culture device according to claim 8, characterized in that The angle α is selected from any angle between 1° and 20°.

10. The culture device according to any one of claims 1 to 2, characterized in that: The angle A between the bottom edge of the culture container and the rotation axis during flipping is 30° to 60°.

11. The culture device according to claim 10, characterized in that: When the medium is replaced, the angle B between the cell adherent culture surface and the horizontal plane is 100° to 150°.

12. The culture device according to any one of claims 1 to 2, characterized in that: The parts of the culture liquid tube and the liquid passage tube extending into the culture container have a bent section. At the liquid replacement position, the ends of the culture liquid tube and the liquid passage tube are located at the bottom of the culture container.

13. The culture device according to claim 12, characterized in that: The ends of the culture liquid tube and the liquid passage tube are covered with silica gel sleeves.

14. The culture device according to any one of claims 1 to 2, characterized in that: The collecting container comprises a washing liquid container and a waste liquid container, and the washing liquid container and the waste liquid container are connected with the culture container through the liquid passage pipe.

15. The culture device according to any one of claims 1 to 2, characterized in that: The culture container, the culture liquid portion, the collection container, and the pump unit are supported by a well plate.

16. A cover assembly, characterized in that: For use in the culture device as described in any one of claims 1-15, the cover assembly includes a cover, and a pipeline arranged on the cover, the pipeline includes at least a culture liquid tube and a liquid passage tube, and the cover seals the container body.

17. A culture container, characterized in that: The culture device as described in any one of claims 1 to 15 comprises a container body and a cover body sealing the container body, wherein the cover body is provided with at least a culture liquid tube and a liquid passage tube.

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