Escherichia coli culture device capable of supplementing materials for multiple times

Abstract

The utility model discloses an escherichia coli culture device capable of supplementing materials for multiple times. The Escherichia coli culture device capable of supplementing materials for multiple times comprises a culture dish box and a culture dish cover, an isolating inner ring cylinder is integrally mounted in the culture dish box, an overflow port is formed in the isolating inner ring cylinder, and a first isolating plate and a second isolating plate are integrally mounted on the isolating inner ring cylinder and the inner wall of the culture dish box. According to the Escherichia coli culture device capable of supplementing materials for multiple times provided by the utility model, the isolation inner ring cylinder is arranged in the culture dish box, and the first isolation plate and the second isolation plate are arranged between the culture dish box and the isolation inner ring cylinder, so that a liquid discharge cavity is formed among the isolation inner ring cylinder, the first isolation plate, the culture dish box and the second isolation plate; and the bottom of the liquid discharge cavity is arranged to be an inclined plane, so that the discharged culture liquid can be automatically and continuously collected towards the liquid extraction tank, and the residue of the waste culture liquid can be reduced as much as possible.

Description

Technical Field

[0001] This utility model relates to the technical field of Escherichia coli culture devices, and in particular to an Escherichia coli culture device that can be fed multiple times. Background Technology

[0002] In microbiological research and biotechnology applications, *Escherichia coli* is a commonly used engineered bacterium. Its rapid growth and ease of genetic manipulation make it an ideal host for recombinant protein production and gene expression studies. However, *E. coli* cultivation often requires multiple replenishments of culture medium to support its continuous growth, especially during long-term or large-scale cultivation. Traditional *E. coli* culture devices typically cannot achieve multiple efficient and aseptic replenishments in a closed environment, which limits cultivation efficiency and increases the risk of contamination.

[0003] A document with publication number CN221566141U discloses a multi-feed Escherichia coli culture device, relating to the technical field of Escherichia coli culture devices. Its structure includes a culture dish box and a culture dish lid; an inner isolating ring is provided on the inner bottom surface of the culture dish box; the bottom of the inner isolating ring has a sloping bottom surface; an overflow port is opened on the upper end surface of the inner isolating ring; the overflow port is located above the top of the sloping bottom surface; a first circular through hole and a second circular through hole are opened on the culture dish lid; a plug is installed on each of the first and second circular through holes. This utility model can improve the efficiency and yield of Escherichia coli culture, while reducing operational complexity and contamination risk, thus bringing improvements and convenience to the fields of microbiology research and biotechnology applications; the multi-feed Escherichia coli culture device provides an efficient, safe, and convenient way to culture and study Escherichia coli, especially suitable for experiments requiring long-term culture and multiple feedings.

[0004] However, when new culture medium is added to the device, the original culture medium is discharged from the overflow port to the outside of the inner ring cylinder. However, since the bottom of the culture dish box outside the inner ring cylinder is flat, it is impossible to remove as much of the overflowed culture medium as possible using a pipette, resulting in too much culture medium remaining at the bottom of the culture dish box.

[0005] Therefore, it is necessary to provide an Escherichia coli culture device that can be fed multiple times to solve the above-mentioned technical problems. Utility Model Content

[0006] In view of the above situation and to overcome the defects of the existing technology, this utility model provides an Escherichia coli culture device that can be replenished multiple times, which can automatically collect the discharged culture medium and reduce waste liquid residue.

[0007] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0008] A refillable Escherichia coli culture device includes: a culture dish box and a culture dish lid. An inner isolation ring is integrally installed inside the culture dish box. An overflow port is provided on the inner isolation ring. A first isolation plate and a second isolation plate are integrally installed between the inner isolation ring and the inner wall of the culture dish box, so that a drainage chamber is formed between the inner isolation ring, the first isolation plate, the culture dish box and the second isolation plate. The bottom height of the drainage chamber gradually decreases from the first isolation plate to the second isolation plate. A suction groove is provided at the lowest point of the drainage chamber.

[0009] Preferably, the petri dish lid has an injection hole and a suction hole, and both the injection hole and the suction hole are fitted with sealing plugs, with the suction hole aligned with the suction tank.

[0010] Preferably, the bottom of the petri dish box is provided with an injection groove, which is aligned with the injection hole.

[0011] Preferably, the bottom of the petri dish box is provided with a first guide groove and multiple second guide grooves of different lengths.

[0012] Preferably, a third isolation plate is installed between the inner isolation ring and the petri dish box, and the bottom of the third isolation plate is provided with a passage groove.

[0013] Preferably, the top of the petri dish box has an isolation groove, and a sealing ring is installed at the bottom of the petri dish lid.

[0014] Preferably, the inner side of the isolation groove is higher than the outer side, and a sealing fluid is injected into the isolation groove.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] (1) This utility model installs an inner isolation ring inside the culture dish box and installs a first isolation plate and a second isolation plate between the culture dish box and the inner isolation ring, so that a drainage chamber is formed between the inner isolation ring, the first isolation plate, the culture dish box and the second isolation plate. The bottom of the drainage chamber is set as an inclined surface, so that the discharged culture solution can automatically and continuously collect in the extraction tank, thereby minimizing the amount of waste culture solution remaining.

[0017] (2) This utility model provides an injection groove at the bottom of the culture dish box, so that the newly added culture medium can gradually replace the original culture medium from this position;

[0018] (3) By providing a first guide groove and a second guide groove at the bottom of the culture dish box, this utility model can improve the flow direction of the newly added culture medium;

[0019] (4) By setting a third isolation plate, this utility model can reduce the exchange of air between the inner ring cylinder and the outside air, thereby reducing the possibility of pollution.

[0020] (5) By setting an isolation groove and a sealing ring, this utility model can easily seal the petri dish box and the petri dish lid;

[0021] (6) By setting the inner side of the isolation groove to be higher than the outer side and injecting sealing liquid into the isolation groove, this utility model can improve the sealing performance between the sealing ring and the isolation groove. Attached Figure Description

[0022] Figure 1 A schematic diagram of the culture dish box in the Escherichia coli culture device that can be replenished multiple times according to this utility model;

[0023] Figure 2 for Figure 1 The diagram shows the structure of the petri dish lid in the E. coli culture device that can be fed multiple times.

[0024] Figure 3 for Figure 1 The diagram shows the structure of the petri dish lid in the E. coli culture device that can be fed multiple times.

[0025] Figure 4 for Figure 1 The diagram shows a cross-sectional view of the culture dish box in the E. coli culture device that can be replenished multiple times.

[0026] The corresponding names of the attached figures are as follows: 1-Cultural dish box, 2-Cultural dish lid, 3-Inner isolation ring, 4-Overflow port, 5-First isolation plate, 6-Second isolation plate, 7-Drainage chamber, 8-Suction groove, 9-Injection hole, 10-Suction hole, 11-Sealing plug, 12-Injection groove, 13-First guide groove, 14-Second guide groove, 15-Third isolation plate, 16-Pass groove, 17-Isolation groove, 18-Sealing ring. Detailed Implementation

[0027] The present invention will be further described below with reference to the accompanying drawings and embodiments. The embodiments of the present invention include, but are not limited to, the following embodiments.

[0028] like Figure 1-4As shown, the present invention provides a refillable Escherichia coli culture device, comprising: a culture dish box 1 and a culture dish lid 2. The connection between the culture dish box 1 and the culture dish lid 2 can adopt the structure disclosed in CN221566141U, which will not be described in detail here. An isolation inner ring cylinder 3 is integrally installed inside the culture dish box 1. The bottom design of the culture dish box 1 inside the isolation inner ring cylinder 3 adopts the inclined structure disclosed in CN221566141U, which will not be described in detail here. An overflow port 4 is provided on the isolation inner ring cylinder 3. The isolation inner ring cylinder 3 and the culture dish box 1 are connected in a continuous manner. A first partition plate 5 and a second partition plate 6 are integrally installed between the inner walls of the culture dish box 1, forming a drainage chamber 7 between the inner ring cylinder 3, the first partition plate 5, the culture dish box 1, and the second partition plate 6. The bottom height of the drainage chamber 7 gradually decreases from the first partition plate 5 to the second partition plate 6, so that the discharged culture medium automatically collects towards the second partition plate 6 after entering the drainage chamber 7. A suction groove 8 is provided at the lowest point of the drainage chamber 7. The culture dish cover 2 is provided with an injection hole 9 and a suction hole 10, and sealing plugs are installed in both the injection hole 9 and the suction hole 10. 11. Align the suction port 10 with the suction tank 8. The inner ring cylinder 3 is used for culturing E. coli. The sealing plug 11 must be sterilized during removal and installation to prevent contamination. When using, remove the sealing plug 11 from the injection port 9 and slowly inject the culture medium into the inner ring cylinder 3 (away from the overflow port 4 and close to the bottom of the culture dish 1) using a pipette. This gradually replaces the culture medium around the E. coli colony, ensuring normal growth of E. coli. After multiple additions of culture medium, the liquid level will rise, with the lower layer mainly consisting of newly added medium. The culture medium, with the upper layer mainly consisting of the original culture medium, causes the liquid level in the inner ring cylinder 3 to gradually rise and exceed the overflow port 4. The culture medium above the overflow port 4 flows from the overflow port 4 into the drainage chamber 7 outside the inner ring cylinder 3, and flows along the bottom of the inclined drainage chamber 7, automatically converging towards the extraction tank 8. Then, the sealing plug 11 on the extraction hole 10 is removed, and a pipette is inserted into the extraction hole 10 to extract the collected culture medium from the extraction tank 8. Because the bottom of the culture medium in the drainage chamber 7 is inclined, there is less culture medium remaining at the bottom of the drainage chamber 7.

[0029] By installing an inner isolation ring 3 inside the culture dish box 1, and installing a first isolation plate 5 and a second isolation plate 6 between the culture dish box 1 and the inner isolation ring 3, a drainage chamber 7 is formed between the inner isolation ring 3, the first isolation plate 5, the culture dish box 1 and the second isolation plate 6. The bottom of the drainage chamber 7 is set as an inclined surface, so that the discharged culture medium can automatically and continuously collect into the extraction tank 8, thereby minimizing the amount of waste culture medium residue.

[0030] Example 2:

[0031] like Figure 1As shown, an injection groove 12 is provided at the bottom of the petri dish box 1. The injection groove 12 is aligned with the injection hole 9. The petri dish box 1 is located at the bottom within the range of the inner ring cylinder 3. It gradually rises from the injection groove 12 towards the overflow port 4, so that the pipette continuously injects culture medium from the injection hole 9 towards the injection groove 12. The culture medium flows along the inclined bottom surface, slowly pushing the original culture medium from the low point to the high point, so that the culture medium around the E. coli colony is replaced.

[0032] By providing an injection groove 12 at the bottom of the culture dish box 1, the newly added culture medium can gradually replace the original culture medium from this position.

[0033] Example 3:

[0034] like Figure 1 and Figure 4 As shown, a first guide groove 13 and multiple second guide grooves 14 of varying lengths are provided at the bottom of the petri dish box 1. The first guide groove 13 is connected to the injection tank 12, and the first guide groove 13 is connected to the second guide groove 14. One end of the first guide groove 13 and the second guide groove 14 both point to the overflow port 4. In use, newly injected culture medium is injected into the injection tank 12. The newly injected culture medium flows along the direction of the first guide groove 13 and the second guide groove 14, making the flow direction of the newly added culture medium more clear and stronger, which facilitates improving the propulsion speed and directionality of the newly added culture medium.

[0035] By providing a first guide groove 13 and a second guide groove 14 at the bottom of the culture dish box 1, the flow direction of the newly added culture medium can be improved.

[0036] Example 4:

[0037] like Figure 1 As shown, a third isolation plate 15 is installed between the inner isolation ring 3 and the culture dish box 1. The bottom of the third isolation plate 15 is provided with a passage groove 16. In use, the third isolation plate 15 reduces the gas exchange between the liquid extraction hole 10 and the inner isolation ring 3 through the overflow port 4. The specific principle has been disclosed in CN221566141U and will not be repeated here.

[0038] By setting a third isolation plate 15, the exchange of air between the inner ring cylinder 3 and the outside air can be reduced, thereby reducing the possibility of pollution.

[0039] Example 5:

[0040] like Figure 1 and Figure 4 As shown, an isolation groove 17 is provided on the top of the petri dish box 1, and a sealing ring 18 is installed at the bottom of the petri dish lid 2. When the petri dish lid 2 is fastened and installed on the petri dish box 1, the sealing ring 18 is embedded in the isolation groove 17, so that the petri dish box 1 and the petri dish lid 2 are sealed.

[0041] By setting the isolation groove 17 and the sealing ring 18, it is easy to seal the petri dish box 1 and the petri dish lid 2.

[0042] Example 6:

[0043] like Figure 4 As shown, the top of the petri dish box 1 is higher inside the isolation groove 17 than outside the isolation groove 17. In use, an appropriate amount of sealing liquid is injected into the isolation groove 17. When the sealing ring 18 is embedded in the sealing groove 17, the excess sealing liquid is squeezed out of the isolation groove 17 and discharged from the lower outer side of the isolation groove 17. The use of sealing liquid improves the sealing performance between the sealing ring 18 and the isolation groove 17.

[0044] By setting the inner side of the isolation groove 17 to be higher than the outer side and injecting sealing fluid into the isolation groove 17, the sealing performance between the sealing ring 18 and the isolation groove 17 can be improved.

[0045] Working principle: When in use, the culture medium flows from the overflow port 4 into the drainage chamber 7 outside the inner ring cylinder 3, and flows along the bottom of the inclined drainage chamber 7, automatically collecting towards the extraction tank 8. Then, the sealing plug 11 on the extraction hole 10 is removed, and a pipette is inserted into the extraction hole 10 to extract the collected culture medium from the extraction tank 8. Because the bottom of the culture medium in the drainage chamber 7 is inclined, less culture medium remains at the bottom of the drainage chamber 7.

Claims

1. A multiple fed-batch E. coli cultivation apparatus, characterized by, Include: Culture dish box (1) and culture dish cover (2); The culture dish box (1) is integrally installed with an isolation inner ring cylinder (3), the isolation inner ring cylinder (3) is provided with an overflow port (4), the isolation inner ring cylinder (3) and the inner wall of the culture dish box (1) are integrally installed with a first isolation plate (5) and a second isolation plate (6), so that the isolation inner ring cylinder (3), the first isolation plate (5), the culture dish box (1) and the second isolation plate (6) form a drainage cavity (7), the bottom height of the drainage cavity (7) gradually decreases from the first isolation plate (5) to the second isolation plate (6), and the lowest part of the drainage cavity (7) is provided with a liquid suction groove (8).

2. The E. coli cultivation device according to claim 1, wherein The culture dish cover (2) is provided with a liquid injection hole (9) and a liquid suction hole (10), the liquid injection hole (9) and the liquid suction hole (10) are provided with sealing plugs (11), and the liquid suction hole (10) is aligned with the liquid suction groove (8).

3. The E. coli cultivation device capable of multiple feedings according to claim 2, wherein The bottom of the culture dish box (1) is provided with an injection groove (12), and the injection groove (12) is aligned with the liquid injection hole (9).

4. The E. coli cultivation device according to claim 1, wherein The bottom of the culture dish box (1) is provided with a first guide groove (13) and a plurality of second guide grooves (14) with different lengths.

5. The E. coli cultivation device according to claim 1, wherein The third isolation plate (15) is installed between the isolation inner ring cylinder (3) and the culture dish box (1), and the bottom of the third isolation plate (15) is provided with a through groove (16).

6. The E. coli cultivation device according to claim 2, wherein The top of the culture dish box (1) is provided with an isolation groove (17), and the bottom of the culture dish cover (2) is provided with a sealing ring (18).

7. The multiple fed-batch E. coli cultivation device according to claim 6, wherein, The inner side of the isolation groove (17) is higher than the outer side, and the isolation groove (17) is filled with sealing liquid.

Images