Fixing device suitable for living animal multi-organ microscopic imaging

Abstract

The utility model provides a fixing device suitable for microimaging of multiple organs of a living animal, which comprises a bottom plate fixedly mounted on a test bed; the sliding grooves are formed in the left and right sides of the bottom plate; the clamping arms are mounted on the sliding grooves in a sliding manner; the observation part is arranged on the clamping arm; the miniature lifting platform is fixedly mounted on the bottom plate; and the temperature control board is mounted at the top end of the micro lifting platform. The fixing mode is firm, looseness is not prone to occurring, living animals of various body types can be fixed, and the influence of the change of the body temperature of the living animals on experimental data can be avoided.

Description

Technical Field

[0001] The utility model belongs to the field of scientific research, and specifically relates to a fixing device suitable for multi-organ microscopic imaging of living animals. Background Art

[0002] In the field of neuroscience-related research, mice and other experimental subjects are usually used as research subjects. The traditional method is to observe animal tissues mainly through tissue section staining. This method requires the animal to be killed, and the tissues to be fixed, sliced ​​and stained. It is impossible to observe the pathological and physiological changes of animal tissues in real time and dynamically. With the widespread application of two-photon microscopy in the field of neurobiology, it has become possible to perform in vivo imaging of animals.

[0003] Real-time observation of physiological and pathological processes of living tissues (such as the brain, spinal cord, kidneys, spleen and other solid organs) under microscopic imaging conditions has always been an important research topic in the fields of neurobiology, immunology, developmental biology, etc. These imaging experiments basically require the experimental subject to be firmly fixed and the observation window to be positioned. If the experimental subject is not firmly fixed, it may cause organ tissue damage or even death of the experimental subject. However, no manufacturer has developed and provided a more practical product between experimental animals and microscopes, such as animal fixation devices for live imaging. Traditional fixation devices cannot firmly fix experimental animals, and the fixation operation is also cumbersome; traditional fixation devices do not have a heat preservation function for experimental subjects, and often directly place the experimental subjects on a cold bottom plate, which is not conducive to long-term experimental observation and may even affect the accuracy of experimental results; and for some specific experimental needs, such as the need to adjust the height between the placement bottom plate and the clamping surface due to the different sizes of living animals, traditional fixation devices lack relevant functions.

[0004] The existing invention patent with patent number 202210432274.2 provides a constant temperature fixation device for living animals, and the technical solution provides a fixing plate with fixing holes and a corresponding temperature control system;

[0005] The utility model patent with patent number 202321875159.9 provides a head fixation device for animal experiments. This technical solution places the fixing plate by means of a slot, which is too simple in structure and has the risk of slipping;

[0006] The invention patent with patent number 202110686690.0 provides a fixed observation device for in vivo imaging of animal spinal nerves and its use method. This technical solution fixes the animal spinal vertebrae by clamping with a clamp, and the clamping force is small and easy to loosen.

[0007] The above-mentioned prior arts all clamp and fix local organs, and are unable to adjust the distance between the clamping surface and the storage bottom plate according to the body size differences of experimental animals. Utility Model Content

[0008] 1. Technical issues to be resolved

[0009] In view of the above technical problems, the utility model provides a fixing device suitable for multi-organ microscopic imaging of living animals. The fixing method is reliable and not easy to loosen. It can fix living animals of various sizes and prevent the changes in the body temperature of the living animals from affecting the experimental data.

[0010] (II) Technical solution

[0011] The utility model provides a fixing device suitable for multi-organ microscopic imaging of living animals, comprising:

[0012] Bottom plate, fixedly mounted on the test bench;

[0013] Sliding grooves are provided on the left and right sides of the bottom plate;

[0014] A clamping arm is slidably mounted on the sliding groove;

[0015] An observation portion, disposed on the clamping arm;

[0016] Miniature lifting platform, fixedly mounted on the base plate;

[0017] A temperature control panel is installed on the top of the micro lifting platform.

[0018] In some embodiments of the present invention, the observation portion includes:

[0019] A titanium alloy sheet, disposed on the clamping arm;

[0020] A pressing block is arranged at the left and right ends of the titanium alloy sheet;

[0021] The first clamping screw fixes the clamping block and the titanium alloy sheet on the clamping arm.

[0022] In some embodiments of the present invention, the observation portion includes:

[0023] Observation ring lower support plate, set on the clamping arm;

[0024] The upper pressure plate of the observation ring is arranged on the lower support plate of the observation ring;

[0025] The organ observation ring is arranged between the lower support plate of the observation ring and the upper pressure plate of the observation ring;

[0026] The second tightening screw fixes the lower support plate of the observation ring, the upper pressure plate of the observation ring and the organ observation ring to the clamping arm.

[0027] In some embodiments of the present invention, bottom plate clamping blocks are provided on the left and right sides of the bottom plate, and the bottom plate clamping blocks are fixed to the test bench by third clamping screws.

[0028] In some embodiments of the utility model, an inverted T-screw is provided inside the sliding groove, the T-screw passes through the clamping arm and is slidably installed inside the sliding groove, and a hand nut is provided above the T-screw, and the hand nut fixes the position of the clamping arm.

[0029] In some embodiments of the present invention, a height-adjustable hand wheel is provided on the front of the miniature lifting platform.

[0030] In some embodiments of the present invention, the temperature control plate has a built-in heating wire.

[0031] (III) Beneficial effects

[0032] It can be seen from the above technical solution that the utility model has the following beneficial effects:

[0033] The clamping arm of the utility model can move forward and backward in the sliding groove and be locked and fixed by T-screws and hand nuts, so as to realize the change of the clamping position of different organs and tissues; the micro-lifting platform is fixed on the bottom plate, and its upper table can move up and down in the Z-axis direction, so as to realize functions such as adjusting the height between the storage table and the clamping surface due to the different body shapes of living animals; a temperature control board is fixed on the table of the micro-lifting platform, and the temperature control board can accurately control the body temperature of the living animals during experimental operations, which is conducive to long-term experimental observation and avoids the influence of changes in body temperature on experimental data; the utility model fixes the living animals through the observation part and the clamping arm, and the fixing method is relatively firm and not easy to loosen. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 It is a schematic diagram of the three-dimensional structure of Example 1 of the utility model.

[0035] Figure 2 It is a schematic diagram of the three-dimensional structure of Example 2 of the utility model.

[0036] Figure 3 It is a top view of embodiment 2 of the utility model.

[0037] Figure 4 It is a front view of embodiment 2 of the utility model.

[0038] Figure 5 It is a side view of embodiment 2 of the present utility model.

[0039] [Description of the main components of this utility model]

[0040] 1. Bottom plate; 2. Sliding groove; 3. Clamping arm;

[0041] 4. Micro lifting platform; 5. Temperature control board; 6. Bottom plate pressing block;

[0042] 7. The third clamping screw; 8. T-type screw; 9. Hand-tightening nut;

[0043] 10. Adjusting hand wheel; 101. Titanium alloy sheet; 102. Pressing block;

[0044] 103, first clamping screw; 201, lower support plate of observation ring; 202, upper pressure plate of observation ring; 203. Organ observation ring; 204. Second clamping screw. DETAILED DESCRIPTION

[0045] The utility model provides a fixing device suitable for microscopic imaging of multiple organs of living animals. In order to make the purpose, technical scheme and advantages of the utility model clearer, the utility model is further described in detail below in combination with specific embodiments and with reference to the accompanying drawings. Specific embodiments

[0047] Example 1

[0048] like Figure 1 As shown, the utility model provides a fixing device suitable for multi-organ microscopic imaging of living animals, including a bottom plate 1, a sliding groove 2, a clamping arm 3, an observation part, a micro-lifting platform 4 and a temperature control plate 5. The bottom plate 1 is fixedly installed on the test bench, and bottom plate clamping blocks 6 are provided on the left and right sides of the bottom plate 1. The bottom plate clamping blocks 6 are fixed to the test bench by third clamping screws 7. The sliding groove 2 is provided on the left and right sides of the bottom plate 1. An inverted T-shaped screw 8 is provided inside the sliding groove 2. The T-shaped screw 8 passes through the clamping arm 3 and is slidably installed inside the sliding groove 2. A hand-tightening nut 9 is provided above the T-shaped screw 8, and the hand-tightening nut 9 fixes the clamping arm 3, the clamping arm 3 is slidably installed on the sliding groove 2, the observation part is arranged on the clamping arm 3, the observation part includes a titanium alloy sheet 101, a clamping block 102 and a first clamping screw 103, the titanium alloy sheet 101 is arranged on the clamping arm 3, the clamping block 102 is arranged at the left and right ends of the titanium alloy sheet 101, the first clamping screw 103 fixes the clamping block 102 and the titanium alloy sheet 101 on the clamping arm 3, the miniature lifting platform 4 is fixedly installed on the base plate 1, the front side of the miniature lifting platform 4 is provided with an adjustable height adjustment hand wheel 10, the temperature control board 5 is installed on the top of the miniature lifting platform 4, and the temperature control board 5 has a built-in heating wire.

[0049] Example 2

[0050] This embodiment is basically the same as the above embodiment, except that:

[0051] The observation part includes an observation ring lower support plate 201, an observation ring upper pressure plate 202, an organ observation ring 203 and a second clamping screw 204. The observation ring lower support plate 201 is arranged on the clamping arm 3, the observation ring upper pressure plate 202 is arranged on the observation ring lower support plate 201, and the organ observation ring 203 is arranged in the middle position between the observation ring lower support plate 201 and the observation ring upper pressure plate 202. The second clamping screw 204 fixes the observation ring lower support plate 201, the observation ring upper pressure plate 202 and the organ observation ring 203 to the clamping arm 3.

[0052] So far, the present embodiment has been described in detail with reference to the accompanying drawings. Based on the above description, those skilled in the art should have a clear understanding of the present utility model.

[0053] It should be noted that the implementation methods not shown or described in the drawings or the text of the specification are all forms known to ordinary technicians in the relevant technical field and are not described in detail. In addition, the above definitions of each element and method are not limited to the various specific structures, shapes or methods mentioned in the embodiments.

[0054] It should also be noted that this article may provide demonstrations of parameters containing specific values, but these parameters do not need to be exactly equal to the corresponding values, but can be approximated to the corresponding values ​​within an acceptable error tolerance or design constraint. The directional terms mentioned in the embodiments, such as "up", "down", "front", "back", "left", "right", etc., are only reference directions of the accompanying drawings and are not intended to limit the scope of protection of the present utility model. In addition, unless the steps are specifically described or must occur in sequence, the order of the above steps is not limited to those listed above, and can be changed or rearranged according to the desired design. And the above embodiments can be mixed and matched with each other or with other embodiments based on design and reliability considerations, that is, the technical features in different embodiments can be freely combined to form more embodiments.

[0055] The specific embodiments described above further illustrate the purpose, technical solutions and beneficial effects of the present invention. It should be understood that the above description is only a specific embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A fixing device suitable for multi-organ microscopic imaging of living animals, characterized in that: include: Bottom plate, fixedly mounted on the test bench; Sliding grooves are provided on the left and right sides of the bottom plate; A clamping arm is slidably mounted on the sliding groove; An observation portion, disposed on the clamping arm; Miniature lifting platform, fixedly mounted on the base plate; A temperature control panel is installed on the top of the micro lifting platform.

2. A fixing device suitable for multi-organ microscopic imaging of living animals according to claim 1, characterized in that: The observation unit comprises: A titanium alloy sheet, disposed on the clamping arm; A pressing block is arranged at the left and right ends of the titanium alloy sheet; The first clamping screw fixes the clamping block and the titanium alloy sheet on the clamping arm.

3. A fixing device suitable for multi-organ microscopic imaging of living animals according to claim 1, characterized in that: The observation unit comprises: The lower support plate of the observation ring is provided on the clamping arm; The upper pressure plate of the observation ring is arranged on the lower support plate of the observation ring; The organ observation ring is arranged between the lower support plate of the observation ring and the upper pressure plate of the observation ring; The second tightening screw fixes the lower support plate of the observation ring, the upper pressure plate of the observation ring and the organ observation ring to the clamping arm.

4. A fixing device suitable for multi-organ microscopic imaging of living animals according to claim 2 or 3, characterized in that: Bottom plate clamping blocks are provided on the left and right sides of the bottom plate, and the bottom plate clamping blocks are fixed on the test bench by third clamping screws.

5. A fixing device suitable for multi-organ microscopic imaging of living animals according to claim 4, characterized in that: An inverted T-shaped screw is arranged inside the sliding groove, and the T-shaped screw passes through the clamping arm and is slidably installed inside the sliding groove. A hand nut is arranged above the T-shaped screw, and the hand nut fixes the position of the clamping arm.

6. A fixing device suitable for multi-organ microscopic imaging of living animals according to claim 5, characterized in that: The front of the miniature lifting platform is provided with an adjusting hand wheel for adjusting the height.

7. A fixing device suitable for multi-organ microscopic imaging of living animals according to claim 6, characterized in that: The temperature control plate has a built-in heating wire.

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