Paper-based biochip flying ink-jet microdroplet spotting instrument

Abstract

The utility model relates to a flying ink-jet microdroplet sample application instrument for a paper-based biochip. The paper-based biochip flying ink-jet microdroplet sample application instrument comprises a rack, a two-dimensional driving mechanism, a liquid spraying mechanism, a liquid path system and a controller, the two-dimensional driving mechanism comprises an X-axis sliding assembly and a Y-axis sliding assembly which are in sliding fit with each other; the liquid spraying mechanism comprises a Z-axis sliding assembly, a high-precision spraying head and a camera shooting mechanism. The liquid path system is used for providing a sample application solution for the high-precision spray head; the liquid path system comprises a first-stage probe liquid storage tank, a second-stage probe liquid storage tank, an ink pump and a height detection module electrically connected with the ink pump; the first-stage probe liquid storage tank, the ink pump, the second-stage probe liquid storage tank and the high-precision spray head are connected in sequence; and the height detection module is used for detecting the height of the probe liquid storage in the secondary probe liquid storage tank. According to the utility model, the liquid dropping amount of sample application can be controlled to a picoliter level, rapid liquid spraying and high-efficiency sample application are realized through the cooperation of the liquid path system and the high-precision spray head, and the preparation of a row of probe points is completed through one-time lifting.

Description

Technical Field

[0001] The utility model relates to the technical field of biological spotting equipment, and more specifically, to a paper-based biological chip flying inkjet micro-droplet spotting instrument. Background Art

[0002] As a high-throughput DNA analysis and detection method, gene chips are widely used in gene expression, disease diagnosis, drug screening and other fields. The existing preparation processes of biological gene chips are mainly divided into in-situ synthesis method and direct spotting method. The in-situ synthesis method adopts the method of synthesizing bases at fixed points on the substrate to prepare microarray chips; the direct spotting method is to first synthesize probe molecules, use direct spotting, and solidify them on the chip surface in the form of microarrays to prepare microarray chips.

[0003] In recent years, with the popularization and application of PCR technology, the demand for gene array chips has been growing. The existing non-contact automatic spotting instrument spots the probe solution one by one through the spotting head. This preparation method can no longer meet the growing demand. In this regard, Patent No. CN203376337U discloses a biochip spotting instrument, which is composed of "host, three-axis motion platform, injection pump, micro solenoid valve, spotting needle, spotting platform, film carrier platform, touch screen monitor and camera". Among them, the film carrier platform is a movable magnetic type, including a square support with an open end composed of a left bracket, a right bracket and a rear bracket, the left and right brackets are provided with guide rails, the guide rails are concave slides arranged on the inner sides of the left and right brackets, and the rear bracket is provided with a positioning magnet; it also includes a film carrier plate, the film carrier plate is provided with a slider corresponding to the guide rails of the left and right brackets, and the left and right sides of the film carrier plate are provided with a film pressing magnet, and the film pressing magnet is magnetically attracted with a film pressing strip, and a positioning suction block is provided at the top of the film carrier plate corresponding to the positioning magnet, and a bracket is provided at the end of the film carrier plate. The use of a spotting needle allows one sample to be spotted by one needle, and the spotting needle is easy to clean to avoid cross infection.

[0004] However, this type of spotter has the following defects: the biochip spotter uses a spotting needle with limited precision. In order to avoid sample contamination, it can only spot samples one by one, which is slow; it is difficult to meet the working requirements of a high-precision nozzle. Summary of the invention

[0005] In view of the above-mentioned existing technical problems, the utility model provides a paper-based biochip flying inkjet droplet sampler, which adopts a high-precision nozzle to control the droplet volume to the picoliter level, and then combines a three-axis motion platform and a camera mechanism to save time and improve accuracy in the sample spotting process, and realizes the sample spotting preparation of a row of probe points with one lift.

[0006] The above-mentioned purpose of the utility model is achieved through the following technical solutions:

[0007] A paper-based biochip flying inkjet droplet spotter, comprising: a frame; a two-dimensional driving mechanism, the two-dimensional driving mechanism is arranged on the frame, the two-dimensional driving mechanism comprises an X-axis sliding component and a Y-axis sliding component that slide with each other; a liquid spraying mechanism, the liquid spraying mechanism comprises a Z-axis sliding component, a high-precision nozzle arranged on the Z-axis sliding component and a camera mechanism, the Z-axis sliding component is slidably arranged on the two-dimensional driving mechanism; a liquid circuit system, the liquid circuit system is arranged on the two-dimensional driving mechanism, and is used to provide a spotting solution for the high-precision nozzle; the liquid circuit system comprises a primary probe liquid storage tank, a secondary probe liquid storage tank, an ink pump and a height detection module electrically connected to the ink pump, the primary probe liquid storage tank, the ink pump, the secondary probe liquid storage tank and the high-precision nozzle are connected in sequence; the height detection module is used to detect the height of the probe liquid storage in the secondary probe liquid storage tank; a controller, the controller is electrically connected to the two-dimensional driving mechanism, the liquid spraying mechanism and the liquid circuit system respectively.

[0008] Through research, the inventors found that if a higher-precision nozzle is used to replace the existing spotting needle, the existing liquid circuit system will find it difficult to provide a suitable and stable negative pressure for the high-precision nozzle. The inventors further optimized the existing liquid circuit system to provide a stable negative pressure for the high-precision nozzle, thereby achieving stable and accurate spraying of the high-precision nozzle. Through research, the inventors found that when the negative pressure of the high-precision nozzle connected to the liquid circuit system is insufficient, it will cause ink leakage from the nozzle; and when the negative pressure of the high-precision nozzle is too large, it will hinder the ejection of ink droplets. The inventors designed a two-stage probe liquid storage tank, and controlled the height difference between the liquid storage in the secondary probe liquid storage tank and the high-precision nozzle through a height detection module, thereby providing a stable negative pressure to the high-precision nozzle. When the liquid level in the secondary probe liquid storage tank drops, the height detection module detects the change in the liquid level and controls the ink pump to transfer the liquid from the primary probe liquid storage tank to the secondary probe liquid storage tank, so that the liquid level in the secondary probe liquid storage tank and the liquid level of the high-precision nozzle always maintain a suitable and stable liquid level difference, and the negative pressure of the high-precision nozzle tends to be stable based on the liquid level height difference.

[0009] The paper-based biochip flying inkjet droplet spotter provided by the utility model can save time and improve accuracy during the spotting process. During the inkjet spotting process, it is first moved to the approximate position through a two-dimensional drive mechanism, and then fine-tuned in position with the assistance of a camera mechanism, and the Z axis is driven to lower the high-precision nozzle to the optimal height difference with the biochip sample. The Z axis drive is used to achieve high-speed and accurate liquid spraying of a row of sample points under the spray coverage of the high-precision nozzle. After the liquid spraying is completed, it is lifted up to spray the next row, and the preparation of a row of probe points is completed by lifting it up once. The utility model adopts a high-precision nozzle, and its liquid spraying accuracy can be controlled in the picoliter range.

[0010] The spotter provided by the utility model can achieve higher control accuracy, thereby saving the use of spotting solution, and the high-precision nozzle with three-axis drive can more accurately spray the sample points on the sample, solving the problems of poor accuracy and waste of probe solution in the existing biochip spotter using a syringe pump (whose droplet volume accuracy can only reach the nanoliter level), thereby greatly saving the preparation cost of the biochip.

[0011] The paper-based biochip flying inkjet micro-droplet spotting instrument provided by the utility model has an advantage in the speed of liquid spraying. In each process of descending and lifting, how many probe points can be prepared depends on the arrangement of a biochip sample and the liquid spray coverage of the high-precision nozzle. Under a reasonable arrangement of the biochip sample, compared with the existing point-by-point sampling, the preparation process of the probe points can be greatly shortened.

[0012] Preferably, in the present invention, the height difference between the first-level probe liquid storage tank and the nozzle of the high-precision nozzle is between -20 mm and -80 mm.

[0013] Preferably, the Y-axis sliding assembly includes a Y-axis slide rail arranged on the frame, a Y-axis slider slidably arranged on the Y-axis slide rail, and a Y-axis motor for providing a sliding driving force for the Y-axis slider, and the X-axis sliding assembly is connected to the Y-axis slider.

[0014] Preferably, the Y-axis slide rail includes a first Y-axis slide rail and a second Y-axis slide rail arranged parallel to each other, and the Y-axis slider includes a first Y-axis slider arranged on the first Y-axis slide rail and a second Y-axis slider arranged on the second Y-axis slide rail.

[0015] Preferably, the X-axis sliding assembly includes an X-axis support, an X-axis slide rail arranged on the X-axis support, an X-axis extension assembly connected to the X-axis support, and an X-axis motor for providing sliding driving force for the Z-axis sliding assembly, and both ends of the X-axis support are respectively connected to the first Y-axis slider and the second Y-axis slider.

[0016] Preferably, the Z-axis sliding assembly includes a second Z-axis assembly slidably disposed on the X-axis slide rail, a Z-axis slide rail connected to the second Z-axis assembly, a Z-axis slider slidably disposed on the Z-axis slide rail, a first Z-axis assembly connected to the Z-axis slider, and a Z-axis motor for driving the Z-axis slider to slide.

[0017] Preferably, the X-axis extension assembly comprises a first support member, a connecting member and a second support member which are connected to each other, the first support member is higher than the second support member, and the first support member is connected to the X-axis support member and is located in the same horizontal plane.

[0018] Preferably, the first-level probe liquid storage tank is arranged on the first support member, and the second-level probe liquid storage tank is arranged on the second support member.

[0019] Preferably, the high-precision nozzle and the camera mechanism are arranged on the first Z-axis assembly.

[0020] Preferably, the frame includes a tripod and a base plate connected to the tripod, and the two-dimensional driving mechanism is arranged on the base plate.

[0021] Preferably, a one-way valve for preventing backflow of the liquid path is also provided between the primary probe liquid storage tank and the secondary probe liquid storage tank.

[0022] Preferably, the physical precision of the high-precision nozzle can reach 600dpi, and the minimum droplet is 12 picoliters.

[0023] Compared with the prior art, the utility model has the following beneficial effects:

[0024] The utility model provides a paper-based biochip flying inkjet micro-droplet spotter, which realizes high-efficiency spotting through a three-axis motion platform and a high-precision nozzle for rapid liquid spraying, and can complete the preparation of a probe reagent for a 60-person HPV biochip with a size of 154 mm × 229 mm within 10 seconds. The paper-based biochip flying inkjet micro-droplet spotter adopts a high-precision nozzle to control the drop volume to the picoliter level. A high-resolution high-speed camera mechanism is used to monitor the spotting process, detect the spotting effect, micro-droplet changes and spotting area in real time, form a closed-loop control, adjust the liquid path system in real time or repair the problems of spotting errors, omissions, and deficiencies, reduce non-conforming products, and improve the yield and quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 The schematic diagram is a structural diagram of a flying inkjet microdroplet spotter for a paper-based biochip.

[0026] Figure 2 A front view of a flying inkjet droplet sampler for a paper-based biochip.

[0027] Figure 3 A top view of a flying inkjet droplet spotter for a paper-based biochip.

[0028] Figure 4 This is a left view of a paper-based biochip flying inkjet droplet sampler.

[0029] In the figure, 10-frame, 11-bottom plate, 12-first tripod, 13-second tripod, 14-biochip sample, 20-two-dimensional driving mechanism, 21-first Y-axis slide rail, 22-second Y-axis slide rail, 23-X-axis slide rail, 24-first Y-axis slider, 25-second Y-axis slider, 26-X-axis extension assembly, 261-first support member, 262-connector, 263-second support member, 27-X-axis motor, 28-Y-axis motor, 29-X-axis support, 30-liquid spraying mechanism, 31-high-precision nozzle, 32-camera mechanism, 33-first Z-axis assembly, 34-second Z-axis assembly, 35-Z-axis slide rail, 36-Z-axis motor, 37-Z-axis slider, 40-liquid path system, 41-first probe liquid storage tank, 42-one-way valve, 43-ink pump, 44-secondary probe liquid storage tank, 45-first liquid pipe, 46-second liquid pipe. DETAILED DESCRIPTION

[0030] The following embodiments are for illustrative purposes only and should not be construed as limiting the present invention. In order to better illustrate the present embodiment, some parts of the accompanying drawings may be omitted, enlarged or reduced, and do not represent the size of the actual product. For those skilled in the art, it is understandable that some well-known structures and their descriptions may be omitted in the accompanying drawings. The positional relationships described in the accompanying drawings are for illustrative purposes only and should not be construed as limiting the present invention.

[0031] When describing the embodiments of the invention, terms such as "length", "width", "thickness", "height", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside" and "outside" indicating orientation and positional relationships are based on the orientation or positional relationships shown in the relevant drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation. Therefore, the above terms should not be understood as limiting the present invention.

[0032] Example 1 A paper-based biochip flying inkjet microdroplet spotter

[0033] like Figures 1 to 4As shown, a paper-based biochip flying inkjet droplet spotting instrument includes: a frame 10, a two-dimensional driving mechanism 20, a liquid spraying mechanism 30, a liquid circuit system 40 and a controller; the two-dimensional driving mechanism 20 is arranged on the frame 10, and the two-dimensional driving mechanism 20 includes an X-axis sliding component and a Y-axis sliding component that slide with each other; the liquid spraying mechanism 30 includes a Z-axis sliding component, a high-precision nozzle 31 arranged on the Z-axis sliding component and a camera mechanism 32, and the Z-axis sliding component is slidably arranged on the two-dimensional driving mechanism 20; the liquid circuit system 40 is arranged on the two-dimensional driving mechanism 20, and is used to provide a spotting solution for the high-precision nozzle 31; the liquid circuit system 40 includes a primary probe liquid storage tank 41, a secondary probe liquid storage tank 44, an ink pump 43 and a height detection module electrically connected to the ink pump 43, and the primary probe liquid storage tank 41, the ink pump 43, the secondary probe liquid storage tank 44 and the high-precision nozzle 31 are connected in sequence; the height detection module is used to detect the height of the probe liquid storage in the secondary probe liquid storage tank 44. The controller is electrically connected to the two-dimensional driving mechanism 20 , the liquid spraying mechanism 30 and the liquid path system 40 respectively.

[0034] In this embodiment, the Y-axis sliding assembly includes a Y-axis slide rail arranged on the frame 10, a Y-axis slider slidably arranged on the Y-axis slide rail, and a Y-axis motor 28 for providing a sliding driving force for the Y-axis slider, and the X-axis sliding assembly is connected to the Y-axis slider.

[0035] In this embodiment, the Y-axis slide rail includes a first Y-axis slide rail 21 and a second Y-axis slide rail 22 arranged parallel to each other, and the Y-axis slider includes a first Y-axis slider 24 arranged on the first Y-axis slide rail 21 and a second Y-axis slider 25 arranged on the second Y-axis slide rail 22.

[0036] In this embodiment, the X-axis sliding assembly includes an X-axis support 29, an X-axis slide rail 23 arranged on the X-axis support 29, an X-axis extension assembly 26 connected to the X-axis support 29, and an X-axis motor 27 for providing sliding driving force for the Z-axis sliding assembly. The two ends of the X-axis support 29 are respectively connected to the first Y-axis slider 24 and the second Y-axis slider 25.

[0037] In this embodiment, the Z-axis sliding assembly includes a second Z-axis assembly 34 slidably set on the X-axis slide rail 23, a Z-axis slide rail 35 connected to the second Z-axis assembly 34, a Z-axis slider 37 slidably set on the Z-axis slide rail 35, a first Z-axis assembly 33 connected to the Z-axis slider 37, and a Z-axis motor 36 for driving the Z-axis slider 37 to slide.

[0038] In this embodiment, the high-precision nozzle 31 and the camera mechanism 32 are disposed on the first Z-axis assembly 33 .

[0039] In this embodiment, the X-axis extension assembly 26 includes a first support member 261, a connecting member 262 and a second support member 263 connected to each other. The horizontal height of the first support member 261 is higher than that of the second support member 263. The first support member 261 is connected to the X-axis support member 29 and is located on the same horizontal plane.

[0040] In this embodiment, the primary probe liquid storage tank 41 is disposed on the first support member 261 , and the secondary probe liquid storage tank 44 is disposed on the second support member 263 .

[0041] Example 2 A paper-based biochip flying inkjet microdroplet spotting instrument

[0042] like Figures 1 to 4 As shown, a paper-based biochip flying inkjet droplet spotting instrument includes: a frame 10, a two-dimensional driving mechanism 20, a liquid spraying mechanism 30, a liquid circuit system 40 and a controller; the two-dimensional driving mechanism 20 is arranged on the frame 10, and the two-dimensional driving mechanism 20 includes an X-axis sliding component and a Y-axis sliding component that slide with each other; the liquid spraying mechanism 30 includes a Z-axis sliding component, a high-precision nozzle 31 arranged on the Z-axis sliding component and a camera mechanism 32, and the Z-axis sliding component is slidably arranged on the two-dimensional driving mechanism 20; the liquid circuit system 40 is arranged on the two-dimensional driving mechanism 20, and is used to provide a spotting solution for the high-precision nozzle 31; the liquid circuit system 40 includes a primary probe liquid storage tank 41, a secondary probe liquid storage tank 44, an ink pump 43 and a height detection module electrically connected to the ink pump 43, and the primary probe liquid storage tank 41, the ink pump 43, the secondary probe liquid storage tank 44 and the high-precision nozzle 31 are connected in sequence; the height detection module is used to detect the height of the probe liquid storage in the secondary probe liquid storage tank 44. The controller is electrically connected to the two-dimensional driving mechanism 20 , the liquid spraying mechanism 30 and the liquid path system 40 respectively.

[0043] In this embodiment, the Y-axis sliding assembly includes a Y-axis slide rail arranged on the frame 10, a Y-axis slider slidably arranged on the Y-axis slide rail, and a Y-axis motor 28 for providing a sliding driving force for the Y-axis slider, and the X-axis sliding assembly is connected to the Y-axis slider.

[0044] In this embodiment, the Y-axis slide rail includes a first Y-axis slide rail 21 and a second Y-axis slide rail 22 arranged parallel to each other, and the Y-axis slider includes a first Y-axis slider 24 arranged on the first Y-axis slide rail 21 and a second Y-axis slider 25 arranged on the second Y-axis slide rail 22.

[0045] In this embodiment, the X-axis sliding assembly includes an X-axis support 29, an X-axis slide rail 23 arranged on the X-axis support 29, an X-axis extension assembly 26 connected to the X-axis support 29, and an X-axis motor 27 for providing sliding driving force for the Z-axis sliding assembly. The two ends of the X-axis support 29 are respectively connected to the first Y-axis slider 24 and the second Y-axis slider 25.

[0046] In this embodiment, the Z-axis sliding assembly includes a second Z-axis assembly 34 slidably set on the X-axis slide rail 23, a Z-axis slide rail 35 connected to the second Z-axis assembly 34, a Z-axis slider 37 slidably set on the Z-axis slide rail 35, a first Z-axis assembly 33 connected to the Z-axis slider 37, and a Z-axis motor 36 for driving the Z-axis slider 37 to slide.

[0047] In this embodiment, the high-precision nozzle 31 and the camera mechanism 32 are disposed on the first Z-axis assembly 33 .

[0048] In this embodiment, the frame 10 includes a tripod and a bottom plate 11 connected to the tripod, and the two-dimensional driving mechanism 20 is disposed on the bottom plate 11. More specifically, the tripod includes a first tripod 12 and a second tripod 13.

[0049] In this embodiment, the X-axis extension assembly 26 includes a first support member 261, a connecting member 262 and a second support member 263 connected to each other. The horizontal height of the first support member 261 is higher than that of the second support member 263. The first support member 261 is connected to the X-axis support member 29 and is located on the same horizontal plane.

[0050] In this embodiment, the primary probe liquid storage tank 41 is disposed on the first support member 261 , and the secondary probe liquid storage tank 44 is disposed on the second support member 263 .

[0051] Example 3 A paper-based biochip flying inkjet microdroplet spotting instrument

[0052] like Figures 1 to 4 As shown, a paper-based biochip flying inkjet droplet spotting instrument includes: a frame 10, a two-dimensional driving mechanism 20, a liquid spraying mechanism 30, a liquid circuit system 40 and a controller; the two-dimensional driving mechanism 20 is arranged on the frame 10, and the two-dimensional driving mechanism 20 includes an X-axis sliding component and a Y-axis sliding component that slide with each other; the liquid spraying mechanism 30 includes a Z-axis sliding component, a high-precision nozzle 31 arranged on the Z-axis sliding component and a camera mechanism 32, and the Z-axis sliding component is slidably arranged on the two-dimensional driving mechanism 20; the liquid circuit system 40 is arranged on the two-dimensional driving mechanism 20, and is used to provide a spotting solution for the high-precision nozzle 31; the liquid circuit system 40 includes a primary probe liquid storage tank 41, a secondary probe liquid storage tank 44, an ink pump 43 and a height detection module electrically connected to the ink pump 43, and the primary probe liquid storage tank 41, the ink pump 43, the secondary probe liquid storage tank 44 and the high-precision nozzle 31 are connected in sequence; the height detection module is used to detect the height of the probe liquid storage in the secondary probe liquid storage tank 44. The controller is electrically connected to the two-dimensional driving mechanism 20 , the liquid spraying mechanism 30 and the liquid path system 40 respectively.

[0053] In this embodiment, the Y-axis sliding assembly includes a Y-axis slide rail arranged on the frame 10, a Y-axis slider slidably arranged on the Y-axis slide rail, and a Y-axis motor 28 for providing a sliding driving force for the Y-axis slider, and the X-axis sliding assembly is connected to the Y-axis slider.

[0054] In this embodiment, the Y-axis slide rail includes a first Y-axis slide rail 21 and a second Y-axis slide rail 22 arranged parallel to each other, and the Y-axis slider includes a first Y-axis slider 24 arranged on the first Y-axis slide rail 21 and a second Y-axis slider 25 arranged on the second Y-axis slide rail 22.

[0055] In this embodiment, the X-axis sliding assembly includes an X-axis support 29, an X-axis slide rail 23 arranged on the X-axis support 29, an X-axis extension assembly 26 connected to the X-axis support 29, and an X-axis motor 27 for providing sliding driving force for the Z-axis sliding assembly. The two ends of the X-axis support 29 are respectively connected to the first Y-axis slider 24 and the second Y-axis slider 25.

[0056] In this embodiment, the Z-axis sliding assembly includes a second Z-axis assembly 34 slidably set on the X-axis slide rail 23, a Z-axis slide rail 35 connected to the second Z-axis assembly 34, a Z-axis slider 37 slidably set on the Z-axis slide rail 35, a first Z-axis assembly 33 connected to the Z-axis slider 37, and a Z-axis motor 36 for driving the Z-axis slider 37 to slide.

[0057] In this embodiment, the high-precision nozzle 31 and the camera mechanism 32 are disposed on the first Z-axis assembly 33 .

[0058] In this embodiment, the X-axis extension assembly 26 includes a first support member 261, a connecting member 262 and a second support member 263 connected to each other. The horizontal height of the first support member 261 is higher than that of the second support member 263. The first support member 261 is connected to the X-axis support member 29 and is located on the same horizontal plane.

[0059] In this embodiment, the primary probe liquid storage tank 41 is disposed on the first support member 261 , and the secondary probe liquid storage tank 44 is disposed on the second support member 263 .

[0060] In this embodiment, the frame 10 includes a tripod and a bottom plate 11 connected to the tripod, and the two-dimensional driving mechanism 20 is disposed on the bottom plate 11. More specifically, the tripod includes a first tripod 12 and a second tripod 13.

[0061] In this embodiment, a one-way valve 42 for preventing backflow of the liquid path is further provided between the primary probe liquid reservoir 41 and the secondary probe liquid reservoir 44. The height difference between the primary probe liquid reservoir 41 and the nozzle of the high-precision nozzle 31 is -20 mm to -80 mm, and the height difference between the secondary probe liquid reservoir 44 and the nozzle of the high-precision nozzle 31 is more than 10 mm.

[0062] In this embodiment, the primary probe liquid storage tank 41 is disposed on the X-axis extension assembly 26 , and the secondary probe liquid storage tank 44 is placed on the X-axis extension assembly 26 on the same horizontal plane as the X-axis slide rail 23 .

[0063] The operation mode of the utility model is as follows: first, the two-dimensional driving mechanism 20 moves the liquid spraying mechanism 30 to the set approximate position, and then the camera mechanism 32 captures the relative position of the current liquid spraying mechanism 30 relative to the biological sample chip 14 (this part uses a certain set nozzle in the high-precision nozzle 31 and the first rectangular center of the biological sample chip 14 that needs to spray liquid as reference points), and the parameters are calculated by the above two coordinates to make the two-dimensional driving mechanism 20 fine-tune the position, and then drive the Z-axis sliding component to lift down, and then the camera mechanism 32 captures the picture of the biological sample chip 14 again, and calculates the matrix center coordinates that need to spray liquid through the analysis of this picture, and then controls the high-precision nozzle 31 to spray liquid at the corresponding upper liquid spraying port. After the liquid spraying is completed, the two-dimensional driving mechanism 20 quickly fine-tunes the position to the next liquid spraying point, thereby realizing the preparation of a row of probe points by lifting once. The device has a simple structure, and the biological sample chip 14 does not need to be fixed on the position of the "film carrier platform", which improves the sampling efficiency.

[0064] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the utility model and are not intended to limit the scope of protection of the utility model. For ordinary technicians in this field, other different forms of changes or modifications can be made based on the above descriptions and ideas. It is not necessary and impossible to list all implementation methods here. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the utility model should be included in the scope of protection of the claims of the utility model.

Claims

1. A paper-based biochip flying inkjet microdroplet spotter, characterized in that: include: Frame (10); A two-dimensional driving mechanism (20), wherein the two-dimensional driving mechanism (20) is arranged on the frame (10), and the two-dimensional driving mechanism (20) comprises an X-axis sliding assembly and a Y-axis sliding assembly that are slidably matched with each other; A liquid spraying mechanism (30), the liquid spraying mechanism (30) comprising a Z-axis sliding assembly, a high-precision nozzle (31) arranged on the Z-axis sliding assembly, and a camera mechanism (32), the Z-axis sliding assembly being slidably arranged on the two-dimensional driving mechanism (20); A liquid circuit system (40), the liquid circuit system (40) being arranged on the two-dimensional driving mechanism (20) and being used for providing a spotting solution for the high-precision nozzle (31); the liquid circuit system (40) comprising a primary probe liquid storage tank (41), a secondary probe liquid storage tank (44), an ink pump (43) and a height detection module electrically connected to the ink pump (43); the primary probe liquid storage tank (41), the ink pump (43), the secondary probe liquid storage tank (44) and the high-precision nozzle (31) being connected in sequence; and the height detection module being used for detecting the height of the probe liquid storage in the secondary probe liquid storage tank (44); A controller is electrically connected to the two-dimensional driving mechanism (20), the liquid spraying mechanism (30) and the liquid path system (40) respectively.

2. The paper-based biochip flying inkjet droplet spotting instrument according to claim 1, characterized in that: The Y-axis sliding assembly comprises a Y-axis sliding rail arranged on the frame (10), a Y-axis slider slidably arranged on the Y-axis sliding rail, and a Y-axis motor (28) for providing a sliding driving force for the Y-axis slider, and the X-axis sliding assembly is connected to the Y-axis slider.

3. The paper-based biochip flying inkjet droplet spotting instrument according to claim 2, characterized in that: The Y-axis slide rail comprises a first Y-axis slide rail (21) and a second Y-axis slide rail (22) which are arranged parallel to each other, and the Y-axis slider comprises a first Y-axis slider (24) arranged on the first Y-axis slide rail (21) and a second Y-axis slider (25) arranged on the second Y-axis slide rail (22).

4. The paper-based biochip flying inkjet droplet spotting instrument according to claim 3, characterized in that: The X-axis sliding assembly comprises an X-axis support member (29), an X-axis slide rail (23) arranged on the X-axis support member (29), an X-axis extension assembly (26) connected to the X-axis support member (29), and an X-axis motor (27) for providing a sliding driving force for the Z-axis sliding assembly, and the two ends of the X-axis support member (29) are respectively connected to a first Y-axis slider (24) and a second Y-axis slider (25).

5. The paper-based biochip flying inkjet droplet spotting instrument according to claim 4, characterized in that: The Z-axis sliding assembly comprises a second Z-axis assembly (34) slidably arranged on the X-axis slide rail (23), a Z-axis slide rail (35) connected to the second Z-axis assembly (34), a Z-axis slider (37) slidably arranged on the Z-axis slide rail (35), a first Z-axis assembly (33) connected to the Z-axis slider (37), and a Z-axis motor (36) for driving the Z-axis slider (37) to slide.

6. The paper-based biochip flying inkjet droplet spotter according to claim 5, characterized in that: The high-precision nozzle (31) and the camera mechanism (32) are arranged on the first Z-axis component (33).

7. The paper-based biochip flying inkjet droplet spotter according to claim 4, characterized in that: The X-axis extension assembly (26) comprises a first support member (261), a connecting member (262) and a second support member (263) which are connected to each other, the first support member (261) is higher than the second support member (263), and the first support member (261) is connected to the X-axis support member (29) and is located on the same horizontal plane.

8. The paper-based biochip flying inkjet droplet spotting instrument according to claim 7, characterized in that: The first-level probe liquid storage tank (41) is arranged on the first support member (261), and the second-level probe liquid storage tank (44) is arranged on the second support member (263).

9. The paper-based biochip flying inkjet droplet spotting instrument according to claim 1, characterized in that: The frame (10) comprises a tripod and a base plate (11) connected to the tripod, and the two-dimensional driving mechanism (20) is arranged on the base plate (11).

10. The paper-based biochip flying inkjet droplet spotter according to claim 1, characterized in that: A one-way valve (42) for preventing backflow of the liquid path is provided between the first-level probe liquid storage tank (41) and the second-level probe liquid storage tank (44).

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