10-hole culture container capable of quantitatively testing concentration of bacteria in water

Abstract

The utility model discloses a 10-hole culture container capable of quantitatively testing the concentration of bacteria in water, the 10-hole culture container comprises a composite sheet and a transparent plate adhered to the surface of the composite sheet, the composite sheet comprises a felt paper layer, an adhesive layer and a sealing layer which are sequentially stacked and compounded, the sealing layer is close to the transparent plate, and the transparent plate is close to the felt paper layer. Wherein the transparent plate integrally protrudes in a direction far away from the composite sheet at a preset position to form a plurality of incubation bins, and a sample inlet for adding samples into the incubation bins is formed in one end between the transparent plate and the composite sheet. According to the 10-hole culture container capable of quantitatively testing the concentration of bacteria in water, the operation steps can be greatly simplified, the manual operation process is reduced, meanwhile, the experiment time can be shortened, and the experiment efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of water sample measuring equipment, in particular to a 10-hole culture container which can quantitatively test the bacterial concentration in water. Background Art

[0002] As people's living standards gradually improve, they pay more and more attention to the health of drinking water and water use, and conduct sanitary evaluations on drinking water, environmental water sources, medical waste water, beverages, surface water, sea water, river water, groundwater and other water sources. Among them, microorganisms in water, such as coliform bacteria, thermotolerant coliform bacteria, and Escherichia coli are important indicators for evaluating whether water quality is polluted.

[0003] At present, qualitative and quantitative detection of microorganisms in water requires cumbersome preparation processes, such as weighing and dissolving the culture medium, adjusting the pH, and then dispensing it into 10 10ml glass test tubes. Inverting the test tubes and adding small catheters to observe whether the microorganisms produce gas, the culture medium is then sterilized, cooled, inoculated with water samples, and placed in a constant temperature incubator for 24 hours of culture. Finally, Gram staining, microscopic examination, and comparison with the MPN table are used to obtain relevant data. This method not only has a long operation time and a complicated preparation process, but also has too many uncertain factors in the detection process due to excessive manual operations, affecting the accuracy of the test results. Utility Model Content

[0004] One advantage of the utility model is that it provides a 10-hole culture container that can quantitatively test the bacterial concentration in water, which can greatly simplify the operation steps, reduce the manual operation process, and also shorten the experimental time and improve the experimental efficiency.

[0005] In order to achieve at least one of the above advantages of the utility model, the utility model provides a 10-well culture container for quantitatively testing the bacterial concentration in water, comprising a composite sheet and a transparent plate pasted on the surface of the composite sheet, wherein the composite sheet comprises a felt paper layer, a glue layer and a sealing layer stacked in sequence, the sealing layer is close to the transparent plate, wherein the transparent plate protrudes integrally at a predetermined position in a direction away from the composite sheet to form a plurality of incubation chambers, and an inlet for adding samples to the incubation chamber is provided at one end between the transparent plate and the composite sheet.

[0006] According to an embodiment of the utility model, the adhesive layer is a water-based heat-sealing adhesive layer.

[0007] According to an embodiment of the present invention, the sealing layer is an aluminum foil sealing layer.

[0008] According to an embodiment of the present invention, an auxiliary handle is provided on one side of the composite sheet.

[0009] According to an embodiment of the utility model, the hatching bin includes a transverse hatching bin, a longitudinal hatching bin and a surplus hatching bin, and the transverse hatching bin, the longitudinal hatching bin and the surplus hatching bin are distributed according to the Poisson distribution law, and the surplus hatching bin is close to the auxiliary handle, and the transverse hatching bin and the longitudinal hatching bin are far away from the auxiliary handle.

[0010] According to an embodiment of the present invention, the transverse incubation bin and the longitudinal incubation bin are both provided with a supporting groove in the middle.

[0011] According to an embodiment of the present invention, the supporting groove is a trapezoidal groove, and the side close to the composite sheet is an upper bottom, that is, a shorter bottom edge.

[0012] These and other purposes, features and advantages of the present invention are fully reflected in the following detailed description. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 A schematic diagram of the exploded structure of a 10-well culture container in a preferred embodiment of the present application is shown.

[0014] Figure 2 A schematic diagram of the exploded structure of a 10-well culture container in a preferred embodiment of the present application is shown as viewed from above.

[0015] Figure 3 A schematic diagram of the top view of a 10-well culture container according to a preferred embodiment of the present application is shown.

[0016] Figure 4 A table of experimental comparison data in this application is shown. DETAILED DESCRIPTION

[0017] The following description is used to disclose the utility model so that those skilled in the art can implement the utility model. The preferred embodiments described below are only examples, and those skilled in the art can think of other obvious variations. The basic principles of the utility model defined in the following description can be applied to other embodiments, variations, improvements, equivalents, and other technical solutions that do not deviate from the spirit and scope of the utility model.

[0018] Those skilled in the art should understand that, in the disclosure of the specification, the orientation or position relationship indicated by the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc. are based on the orientation or position relationship shown in the drawings, which are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, the above terms should not be understood as limiting the present invention.

[0019] It is to be understood that the term "one" should be understood as "at least one" or "one or more", that is, in one embodiment, the number of an element may be one, while in another embodiment, the number of the element may be multiple, and the term "one" should not be understood as a limitation on the quantity.

[0020] refer to Figures 1 to 3 According to a preferred embodiment of the utility model, a 10-well culture container for quantitatively testing the concentration of bacteria in water will be described in detail below, wherein the 10-well culture container for quantitatively testing the concentration of bacteria in water includes a composite sheet 10 and a transparent plate 20 pasted on the surface of the composite sheet 10, wherein the composite sheet 10 includes a felt paper layer 11, a glue layer 12 and a sealing layer 13 stacked in sequence, and the sealing layer 13 is close to the transparent plate 20, wherein the transparent plate 20 can be a plastic plate, and the transparent plate 20 is integrally protruded at a predetermined position in a direction away from the composite sheet 10 to form a plurality of incubation chambers 201, and at the same time, a sampling port for adding samples to the incubation chamber 201 is provided at one end between the transparent plate 20 and the composite sheet 10.

[0021] Specifically, the water sample mixture is first poured into the incubation chamber 201 through the sampling port, and then moved into the heat sealer together with the fixture, the transparent plate 20 and the composite sheet 10 are bonded together in the heat sealer, wherein the incubation chamber 201 may include a horizontal incubation chamber 21, a vertical incubation chamber 22 and a surplus incubation chamber 23, and the water sample mixture is evenly distributed in each incubation chamber 201, with a capacity of 10-12 ml, and then the water sample type, culture time, and culture project are marked on the outermost layer of the composite sheet 10 with a marker, and then the culture container is placed in a constant temperature incubator at 36°C for 24 hours, and then taken out after the culture is completed, and the number of incubation chambers that turn yellow are observed under indoor lighting, and then placed under a wavelength of 366nm ultraviolet light for observation, and the number of incubation chambers that appear fluorescent is recorded, and the results are obtained through the MPN table. The experimental results are basically consistent with the results of the traditional ten-tube method. Therefore, the culture container can greatly simplify the operation steps and reduce the manual operation process, while also shortening the experimental time and improving the experimental efficiency.

[0022] In one embodiment, the adhesive layer 12 is a water-based heat-sealing adhesive layer. The water-based heat-sealing adhesive is bonded through two processes: softening and curing of the adhesive layer 12. When heated, the thickener and stabilizer in the adhesive layer 12 will cause the colloidal particles to flow again, so that the adhesive layer 12 and the surface of the bonded material produce physical adsorption and molecular diffusion phenomena, thereby forming a strong bond, making the composite sheet have a better composite effect and a more stable structure.

[0023] In one embodiment, the sealing layer 13 is an aluminum foil sealing layer. Aluminum foil is an electrolytic capacitor material and a soft metal film. It is not only moisture-proof, airtight, light-shielding, abrasion-resistant, and waterproof, but also, because the sample is in a liquid state, it can be sealed with aluminum foil to ensure that the sample will not leak out and be sealed in the incubation chamber. At the same time, it can provide the microorganisms with the required light-proof and heat-insulating culture environment during the culture process.

[0024] In one embodiment, an auxiliary handle 14 is provided on one side of the composite sheet 10, so as to facilitate opening the sample inlet when adding the water sample mixture into the culture container.

[0025] In one embodiment, the horizontal incubation chamber 21, the vertical incubation chamber 22 and the surplus incubation chamber 23 are distributed according to the Poisson distribution law, and the surplus incubation chamber 23 is close to the auxiliary handle 14, and the horizontal incubation chamber 21 and the vertical incubation chamber 22 are far away from the auxiliary handle 14, so that the discrete probability of random appearance of microorganisms in a unit time can be relatively uniform. Since the total amount of water sample added is 100ml, the total capacity inside the container is 120ml, and when adding water sample, it is not necessarily possible to control it very accurately at 100ml, the surplus incubation chamber 23 can absorb the excess water sample mixture, thereby ensuring that the sample will not overflow the container, so that the detection result is not missed.

[0026] In one embodiment, the transverse hatching chamber 21 and the longitudinal hatching chamber 22 are both provided with a support groove 202 at the middle position, so that the hatching chamber 201 is not easily deformed and the space inside the hatching chamber 201 can be made more stable.

[0027] Further preferably, the supporting groove 202 is a trapezoidal groove, and the side close to the composite sheet 10 is the upper bottom, that is, the shorter bottom side.

[0028] Comparative experiment: The ten-tube method in the prior art was used for comparison. Based on the normal distribution principle, the number of samples N>30, 30 groups of valid samples were prepared and divided into two groups. Paired T test was performed on the present invention and the ten-tube method to analyze whether there was a significant statistical difference between the two. The data between the two were equivalent.

[0029] Experimental method: Take the standard strain Escherichia coli with a concentration of 10-50 CFU, strain number ATCC 25922, MMO-MUG culture medium, in accordance with GB / T5750.12-2023 "Standard Test Methods for Microbial Indicators of Drinking Water".

[0030] Take 100 ml of water sample, add enzyme substrate culture medium, and pour into the present invention; culture at 36°C±1°C for 24 hours.

[0031] Take 100 ml of water sample, add enzyme substrate culture medium, add it into 10 test tubes, 10 ml in each tube, and culture at 36℃±1℃ for 24 hours.

[0032] Results: The sample color changes to magenta for the present application technology, and changes to yellow for the conventional ten-tube method technology. Blue fluorescence is produced under a 366nm wavelength ultraviolet lamp, indicating that it is positive for Escherichia coli. Find the MPN table to record the results.

[0033] Comparative data such as Figure 4 ;

[0034] The comparison of the logarithmic pairing variables of the present invention and the ten-tube method in the experiment is shown in the following figure:

[0035] Paired Sample Statistics

[0036] average value Number Standard Deviation Standard error of the mean Ten-pipe method .7927 40 0.31845 0.05035 The present invention .7867 40 0.29989 0.04742

[0037] Paired Samples Correlation

[0038] Number of cases Relevance Significance Ten-tube method & the present invention 40 .952 .000

[0039] Paired Samples Test

[0040]

[0041] In summary, from the paired sample correlation table, it can be seen that the correlation between the present invention and the ten-tube method is 0.952 (range 0-1.000, the closer the result is to 1.000, the stronger the correlation between the two);

[0042] From the paired sample test table, it can be seen that the significance (two-tailed) of the present invention and the ten-tube method is Sig, and the P value result is P=0.702>0.05, which means that there is no significant difference. It can be determined that the two methods do not have a statistically significant difference, that is, the present invention and the ten-tube method are no different and are equivalent.

[0043] At the same time, the technology of the present application can greatly simplify the operating steps during the experiment, reduce the manual operation process, shorten the experimental time, and improve the experimental efficiency.

[0044] It should be understood by those skilled in the art that the embodiments of the present invention described above and shown in the accompanying drawings are only examples and do not limit the present invention. The advantages of the present invention have been fully and effectively realized. The functions and structural principles of the present invention have been demonstrated and explained in the embodiments, and the embodiments of the present invention may be changed or modified in any way without departing from the principles.

Claims

1. A 10-well culture container capable of quantitatively testing the bacterial concentration in water, characterized in that: It comprises a composite sheet and a transparent plate adhered to the surface of the composite sheet, wherein the composite sheet comprises a felt paper layer, a glue layer and a sealing layer which are stacked and composited in sequence, the sealing layer is close to the transparent sheet, wherein the transparent sheet protrudes integrally at a predetermined position in a direction away from the composite sheet to form a plurality of incubation chambers, and an inlet for adding samples to the incubation chambers is arranged at one end between the transparent sheet and the composite sheet.

2. The 10-well culture container for quantitatively testing the bacterial concentration in water as claimed in claim 1, characterized in that: The adhesive layer is a water-based heat-sealing adhesive layer.

3. The 10-well culture container for quantitatively testing the bacterial concentration in water as claimed in claim 1, characterized in that: The sealing layer is an aluminum foil sealing layer.

4. The 10-well culture container for quantitatively testing the bacterial concentration in water as claimed in claim 1, characterized in that: An auxiliary handle is provided on one side of the composite sheet.

5. The 10-well culture container for quantitatively testing the bacterial concentration in water as claimed in claim 4, characterized in that: The hatching bin comprises a transverse hatching bin, a longitudinal hatching bin and a surplus hatching bin, wherein the transverse hatching bin, the longitudinal hatching bin and the surplus hatching bin are distributed according to the Poisson distribution law, and the surplus hatching bin is close to the auxiliary handle, while the transverse hatching bin and the longitudinal hatching bin are far away from the auxiliary handle.

6. The 10-well culture container for quantitatively testing the bacterial concentration in water as claimed in claim 5, characterized in that: The transverse hatching bin and the longitudinal hatching bin are both provided with a supporting groove in the middle.

7. The 10-well culture container for quantitatively testing the bacterial concentration in water as claimed in claim 6, characterized in that: The supporting groove is a trapezoidal groove, and the side close to the composite sheet is an upper bottom, that is, a shorter bottom edge.

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