Sampling device for in-situ microorganism enrichment of submarine sediments
By designing a sampling device for in-situ microbial enrichment of seabed sediments, the problem that the existing technology is difficult to simulate the seabed environment is solved, efficient microbial enrichment and regular injection of nutrient solution are achieved, and the efficiency of microbial culture is improved.
Patent Information
- Application Number
- CN202421166216.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-27
- Publication Date
- 2025-03-28
- Estimated Expiration
- 2034-05-27
AI Technical Summary
The existing microbial enrichment culture device is difficult to truly simulate the seabed environment, resulting in inefficient microbial culture.
A sampling device for in-situ microbial enrichment of seabed sediments is designed, including sampling tubes, petal valves, capillaries and reaction medium storage mechanisms, to achieve in-situ microbial enrichment and periodic injection of enriched culture nutrient solution through optimized structure.
It achieves efficient enrichment of in situ microorganisms, simulates the seabed environment, improves the contact efficiency between microorganisms and nutrient solution, ensures the long-term nutritional supply of microorganisms, and discovers the differences between microorganisms at different depths.
Smart Images
Figure CN222684624U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of microbial enrichment and cultivation, in particular to a sampling device for seabed sediments. Background Art
[0002] The ocean is rich in microbial resources, with extremely high species diversity and genetic diversity. However, due to the limitations of laboratory enrichment and culture technology, less than 1% of microorganisms can be isolated and cultured in the laboratory, resulting in insufficient understanding of the metabolic functions and resource conditions of marine microorganisms. The inability of laboratories to truly simulate the seabed environment and the slow growth of seabed microorganisms are two major reasons for the difficulty in culturing microorganisms.
[0003] At present, most microbial enrichment and culture devices are used in seawater environments, and there is still a lack of regular in-situ culture devices for seabed sediments. Due to the poor fluidity of sediments, it is difficult for the enrichment culture substrate to fully contact the sediment, resulting in low efficiency of microbial enrichment and culture. Utility Model Content
[0004] In view of the problems existing in the prior art, the utility model provides a sampling device for in-situ microbial enrichment of seabed sediments, which solves at least one of the above technical problems.
[0005] The technical solution of the utility model is: a sampling device for in-situ microbial enrichment of seabed sediments, comprising a sampling tube, characterized in that the lower end of the sampling tube is open, and the lower end of the sampling tube is connected with a petal valve that bulges upward;
[0006] The inner cavity of the sampling tube is used as a sample enrichment chamber, a vertically arranged capillary is fixed to the inner wall of the sampling tube, and outlet holes are sequentially opened on the capillary along the axial direction;
[0007] The upper end of the sampling tube is connected to a reaction medium storage mechanism, which includes a shell, in which at least two circumferentially arranged sub-chambers are arranged, each sub-chamber is divided into a driving chamber and a storage chamber arranged up and down by a piston partition, and a driving source for driving the piston partition to squeeze the storage chamber is arranged in the driving chamber;
[0008] Each storage cavity has a guide outlet at the bottom, the guide outlet is connected to the capillary tube, and a valve is installed on the guide outlet;
[0009] A liquid discharge port is provided in the center of the shell, and the liquid discharge port is slidably connected to a drain pipe, on which a valve is installed.
[0010] The utility model optimizes the structure of the sampling device to facilitate in-situ enrichment of microorganisms, and guides the nutrient solution for enrichment culture through the storage cavity, thereby achieving enrichment culture of microorganisms.
[0011] The seawater in the sampling tube can be easily discharged through the drain pipe.
[0012] As a preferred solution, the driving source is an electric driving mechanism, which includes a motor, a control circuit board and a power supply. The power input terminal of the control circuit board is connected to the power supply, and the control circuit board controls the connection to the motor.
[0013] It is convenient to control the operation of the motor through the control circuit board to export the nutrient solution.
[0014] Further preferably, the motor is a linear motor, and the telescopic shaft of the linear motor is connected to the piston partition.
[0015] Alternatively, the motor is a rotary motor, a power output shaft of the motor is connected to a gear, the gear is meshed with a spur rack slidably connected to the drive chamber, and the spur rack is connected to the piston partition.
[0016] It is convenient to realize the movement of the piston partition.
[0017] Further preferably, the petal valve comprises at least five stainless steel valve flaps arranged circumferentially, and the stainless steel valve flaps extend inwardly in an arc shape from bottom to top.
[0018] After the bottom mud is inserted, the stainless steel valve disc is forced to open, and the bottom mud is introduced from between the adjacent stainless steel valve discs. And under the elastic force of the stainless steel valve disc, it rebounds and resets to close.
[0019] Further preferably, the bottom of the sampling tube is threadedly connected to an insertion head, and the end of the insertion head away from the sampling tube is a needle tip structure or a tooth structure.
[0020] It is convenient to insert the sampling tube into the bottom mud.
[0021] Further preferably, the guide outlet is connected to the capillary via a transfer chamber, a through hole connected to the capillary is provided at the bottom of the transfer chamber, and the top of the transfer chamber is connected to the guide outlet one by one.
[0022] Beneficial effects:
[0023] This device can enrich microorganisms in situ, solving the problem that laboratory microbial enrichment cannot truly simulate the seabed environment. In addition, this device regularly injects enrichment culture nutrient solution in layers, which not only ensures the long-term nutrition required by microorganisms, but also can compare and discover the differences of microorganisms at different depths. At the same time, it can allow sediments at different locations to fully contact the nutrient solution, thereby improving the enrichment efficiency. BRIEF DESCRIPTION OF THE DRAWINGS
[0024] Figure 1This is a structural schematic diagram of a specific embodiment 1 of the utility model;
[0025] Figure 2 This is a structural schematic diagram of specific embodiment 3 of the utility model.
[0026] In the figure: 1 is a piston partition, 2 is a driving chamber, 3 is a storage chamber, 4 is a capillary tube, 5 is a transfer chamber, 6 is a petal valve, 7 is an insertion head, 8 is a drain pipe, 9 is a gas pipeline, 10 is a spur rack, and 11 is a driving gear. DETAILED DESCRIPTION
[0027] See also Figure 1 Specific embodiment 1, a sampling device for in-situ microbial enrichment of seabed sediments, comprising a sampling tube, the lower end of which is open, and the lower end of which is connected to a petal valve 6 that bulges upward; the inner cavity of the sampling tube is used as a sample enrichment chamber, and a vertically arranged capillary 4 is fixed on the inner wall of the sampling tube, and outlet holes are sequentially opened on the capillary 4 along the axial direction; the upper end of the sampling tube is connected to a reaction medium storage mechanism, and the reaction medium storage mechanism includes a shell, and at least two circumferentially arranged sub-chambers are arranged in the shell, and each sub-chamber is divided into a driving chamber 2 and a storage chamber 3 arranged up and down by a piston partition 1, and a driving source for driving the piston partition 1 to squeeze the storage chamber 3 is arranged in the driving chamber 2; a guide outlet is provided at the bottom of each storage chamber 3, and the guide outlet is connected to the capillary 4, and a valve is installed on the guide outlet; a liquid discharge port is provided in the center of the shell, and a drain pipe 8 is slidably connected to the liquid discharge port, and a valve is installed on the drain pipe 8. The utility model optimizes the structure of the sampling device to facilitate in-situ microbial enrichment. The nutrient solution for enrichment culture is led out through the storage chamber 3, thereby achieving enrichment culture of microorganisms. The seawater in the sampling tube is conveniently led out through the drain pipe.
[0028] The valve installed at the outlet can be a one-way silicone valve, which is convenient for one-way outlet of the medium in the storage chamber. The valve installed at the drain pipe can be a one-way silicone valve for one-way outward drainage.
[0029] The petal valve 6 includes at least five stainless steel valve flaps arranged circumferentially, and the stainless steel valve flaps extend inward in an arc shape from bottom to top. After the bottom mud is inserted, the stainless steel valve flaps are forced to open, and the bottom mud is introduced from between adjacent stainless steel valve flaps. And under the elastic force of the stainless steel valve flaps, they rebound and reset to close.
[0030] The bottom of the sampling tube is threadedly connected with an insertion head 7, and the end of the insertion head 7 away from the sampling tube is a needle tip structure or a tooth structure.
[0031] The outlet is connected to the capillary 4 through a transfer chamber 5. The bottom of the transfer chamber 5 is provided with a through hole connected to the capillary 4. The top of the transfer chamber 5 is connected to the outlet one by one.
[0032] Specific embodiment 2, based on specific embodiment 1, the driving source is an electric driving mechanism, the electric driving mechanism includes a motor, a control circuit board and a power supply, the power input end of the control circuit board is connected to the power supply, and the control circuit board is controlled to connect to the motor. It is convenient to control the motor to work through the control circuit board to export the nutrient solution.
[0033] The motor is a linear motor, and the telescopic shaft of the linear motor is connected to the piston diaphragm.
[0034] See also Figure 2 Specific embodiment 3, based on specific embodiment 1, the driving source is an electric driving mechanism, the electric driving mechanism includes a motor, a control circuit board and a power supply, the power input end of the control circuit board is connected to the power supply, and the control circuit board controls the connection to the motor. It is convenient to control the motor to work through the control circuit board to export the nutrient solution.
[0035] The motor is a rotary motor, the power output shaft of the motor is connected to the gear 11, the gear 11 is meshed with the spur rack 10 of the sliding connection driving chamber, and the spur rack 10 is connected to the piston partition, so as to facilitate the movement of the piston partition.
[0036] The above are only preferred implementations of the present invention. It should be pointed out that ordinary technicians in this technical field can make several improvements and modifications without departing from the principles of the present invention. These improvements and modifications should also be regarded as the protection scope of the present invention.
Claims
1. A sampling device for in-situ microbial enrichment of seafloor sediments, comprising a sampling tube, characterized in that: The lower end of the sampling tube is open, and the lower end of the sampling tube is connected to a petal valve that bulges upward; The inner cavity of the sampling tube is used as a sample enrichment chamber, a vertically arranged capillary is fixed to the inner wall of the sampling tube, and outlet holes are sequentially opened on the capillary along the axial direction; The upper end of the sampling tube is connected to a reaction medium storage mechanism, which includes a shell, in which at least two circumferentially arranged sub-chambers are arranged, each sub-chamber is divided into a driving chamber and a storage chamber arranged up and down by a piston partition, and a driving source for driving the piston partition to squeeze the storage chamber is arranged in the driving chamber; Each storage cavity has a guide outlet at the bottom, the guide outlet is connected to the capillary tube, and a valve is installed on the guide outlet; A liquid discharge port is provided in the center of the shell, and the liquid discharge port is slidably connected to a drain pipe, on which a valve is installed.
2. A sampling device for in-situ microbial enrichment of seabed sediments according to claim 1, characterized in that: The driving source is an electric driving mechanism, which includes a motor, a control circuit board and a power supply. The power input end of the control circuit board is connected to the power supply, and the control circuit board controls the connection to the motor.
3. A sampling device for in-situ microbial enrichment of seabed sediments according to claim 2, characterized in that: The motor is a linear motor, and the telescopic shaft of the linear motor is connected to the piston partition.
4. A sampling device for in-situ microbial enrichment of seabed sediments according to claim 2, characterized in that: The motor is a rotary motor, a power output shaft of the motor is connected to a gear, the gear is meshed with a spur rack that is slidably connected to the drive chamber, and the spur rack is connected to the piston partition.
5. The sampling device for in-situ microbial enrichment of seabed sediments according to claim 1, characterized in that: The petal valve comprises at least five stainless steel valve flaps arranged circumferentially, and the stainless steel valve flaps extend inwardly in an arc shape from bottom to top.
6. The sampling device for in-situ microbial enrichment of seabed sediments according to claim 1, characterized in that: The bottom of the sampling tube is threadedly connected with an insertion head, and the end of the insertion head away from the sampling tube is a needle tip structure or a tooth structure.
7. The sampling device for in-situ microbial enrichment of seabed sediments according to claim 1, characterized in that: The guide outlet is connected to the capillary tube via a transfer chamber, a through hole connected to the capillary tube is provided at the bottom of the transfer chamber, and the top of the transfer chamber is connected to the guide outlet one by one.