Root-knot nematode infection observation device

Abstract

The utility model discloses a root-knot nematode infection observation device which comprises a tray structure, the tray structure comprises clamping grooves which are evenly arranged and observation pipes which are evenly distributed on the tray structure through the clamping grooves and used for planting rice, and scale marks which are used for measuring the length of a rice root system are arranged on the outer walls of the observation pipes. And the observation tube is made of a transparent material. According to the device, a rice plant does not need to be pulled out for cleaning, so that the rice root system is protected, the accuracy of experimental data is ensured, the length of the rice root system can be measured more quickly and efficiently, and the working intensity of experimenters is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of root-knot nematode infection observation, in particular to a root-knot nematode infection observation device. Background Art

[0002] Root-knot nematodes of the Poaceae family ( Meloidogyne graminicola ) is a soil-borne pathogen on rice. In recent years, the distribution of root-knot nematodes of the Poaceae family has been reported in rice-producing areas such as Hainan, Fujian, Guangdong, Hubei, Hunan, Jiangsu, and Jiangxi in China, seriously affecting rice production.

[0003] The root-knot nematode of the Poaceae family harms rice, mainly infecting the root tips, causing hook-shaped root knots to form on the root tips of rice. The infected above-ground rice plants are short, the leaves turn yellow, and in severe cases, the plants die. Since the nematode has the characteristic of surviving in the form of eggs or larvae in flooded soil, it can not only harm upland rice, but also have certain harm to rice in low-lying land, irrigated land and deep water land. In addition, it has a wide range of hosts, not only infecting rice, but also infecting grass weeds and more than 100 different host plants including cereals and dicotyledons.

[0004] The gramineous root-knot nematode is different from other root-knot nematodes in that the egg sacs of this gramineous root-knot nematode can be buried under the root epidermis of the host, so that the egg sacs are not exposed. Therefore, the life cycle of the gramineous root-knot nematode can be completed without leaving the host.

[0005] Studying the early infection process of the gramineous root-knot nematode is of great significance to the occurrence and spread trend of rice root-knot nematode disease in my country and the prevention and control of the gramineous root-knot nematode disease, and it also provides valuable data and information for further research on the pathogenic mechanism of the gramineous root-knot nematode.

[0006] The conventional method for early observation of rice infection with root-knot nematodes is field experiments or pot experiments. During the experiment, the rice seedlings need to be pulled out of the soil, the rice root tissues need to be cleaned, and then the number of rice root knots and the growth of the root system need to be observed. Since the pulling out and cleaning process inevitably causes some root systems to break, there are certain errors in the observation results. At the same time, the rice used for observation cannot be planted after being pulled out, and the experimental process requires planting seedlings in batches and inoculating nematodes, which is labor-intensive.

[0007] Therefore, how to provide a device for conveniently observing the early infection of rice by Pseudomonas graminicola, ensure the accuracy of observation data, improve the accuracy of experiments, and reduce the workload is a technical problem that needs to be solved urgently. Utility Model Content

[0008] The utility model aims to provide a root-knot nematode infection observation device to solve the problems in the background technology that the existing method for observing early infection of rice by gramineous knot nematodes is prone to errors in the observation results and has high work intensity.

[0009] In order to achieve the above-mentioned purpose, the utility model proposes a root-knot nematode infection observation device, which includes a tray structure, the tray structure includes evenly arranged slots, and observation tubes for planting rice are evenly arranged on the tray structure through the slots. The outer wall of the observation tube is provided with scale lines for measuring the length of the rice root system, and the observation tube is made of a transparent material.

[0010] Optionally, an overhead layer is provided at the bottom of the observation tube, and the height of the overhead layer is greater than the depth of the slot.

[0011] Optionally, a sponge plug is installed at the tube mouth of the observation tube, an observation plane is provided on the outer wall of the observation tube, and a fluid infusion channel is also provided on the observation tube.

[0012] Optionally, the fluid infusion channel includes a fluid infusion port, a fluid outlet provided on the observation tube and connected to the inner cavity of the observation tube, and a connecting channel provided on the observation tube for connecting the fluid infusion port and the fluid outlet.

[0013] Optionally, the fluid infusion port is located on an observation plane and is disposed near a tube opening of the observation tube.

[0014] Optionally, the distance between the lowest point of the liquid outlet and the bottom surface of the inner cavity is not less than 8 cm.

[0015] Optionally, the tray structure further includes a tray, and the card slots are evenly arranged on the tray.

[0016] Optionally, the pallet structure also includes a pallet base, a plurality of movable plates equidistantly spaced and movably mounted on the pallet base, and the card slots are equidistantly arranged on the movable plates; a slide groove corresponding to the movable plate is provided on the pallet base, and a card connection structure is provided between the slide groove and the movable plate.

[0017] Optionally, a mounting groove is provided at the bottom of the tray base, and a connecting groove is provided in the mounting groove; a movable groove is provided on the top surface of the movable plate, and a limiting groove corresponding to the connecting groove is provided in the movable groove; the clamping structure is arranged in the mounting groove, the connecting groove, the movable groove and the limiting groove.

[0018] Optionally, the clamping structure includes a moving block movably installed in the installation groove, a clamping block installed on the moving block and adapted to the connecting groove, a telescopic spring installed in the movable groove, a telescopic block installed in the movable groove, and a magnet installed on the telescopic block and adapted to the limiting groove.

[0019] Optionally, the size of the installation groove is larger than the size of the connecting groove; the size of the movable groove is larger than the size of the limiting groove.

[0020] Optionally, an abutment block in contact with the telescopic spring is provided on the telescopic block away from the magnet, and the abutment block is adapted to the movable slot; a baffle is installed at the slot opening of the movable slot away from the limiting slot.

[0021] Optionally, a sealing plate is installed at the slot opening of the installation slot away from the communicating slot.

[0022] Compared with the prior art, the utility model provides a root-knot nematode infection observation device, which has the following beneficial effects:

[0023] The root-knot nematode infection observation device is made of an observation tube made of a transparent material. Rice is planted in the observation tube, and the early infection process of the root-knot nematode can be directly observed, so that the experimental result is more accurate and reliable. In combination with the setting of the scale line, the length of the rice root system can be directly measured without pulling out the rice plant for cleaning, thereby protecting the rice root system and ensuring the accuracy of the experimental data. The length of the rice root system can be measured more quickly and efficiently, reducing the workload of the experimenter. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a schematic diagram of the overall structure of Example 1 of the utility model.

[0025] Figure 2 It is a structural schematic diagram of the observation tube of the utility model.

[0026] Figure 3 It is a cross-sectional view of the observation tube of the utility model.

[0027] Figure 4 It is a schematic diagram of the overall structure of Example 2 of the utility model.

[0028] Figure 5 It is a schematic diagram of the movable plate and the clamping structure in Example 2 of the utility model.

[0029] Figure 6 It is a schematic diagram of the tray base and the clamping structure in Example 2 of the utility model.

[0030] Markings in the figure: 1. Tray structure; 11. Card slot; 12. Tray; 13. Tray base; 131. Mounting slot; 132. Connecting slot; 133. Closing plate; 14. Moving plate; 141. Movable slot; 142. Limiting slot; 143. Baffle; 15. Slide; 2. Observation tube; 21. Mezzanine; 22. Sponge plug; 23. Observation plane; 3. Scale line; 4. Card connection structure; 41. Moving block; 42. Card connection block; 43. Telescopic spring; 44. Telescopic block; 441. Abutment block; 45. Magnet; 5. Fluid replenishment channel; 51. Fluid replenishment port; 52. Liquid outlet; 53. Connecting channel. DETAILED DESCRIPTION

[0031] The following is a detailed description in conjunction with the accompanying drawings and specific implementations. In the following description, many specific details are set forth to facilitate a full understanding of the present utility. However, the present utility can be implemented in many other ways different from those described herein, and those skilled in the art can make similar generalizations without violating the connotation of the present utility, so the present utility is not limited by the specific embodiments disclosed below.

[0032] The root-knot nematode infection observation device of the present application can be used to observe the early infection of rice by root-knot nematodes of the Poaceae family, and of course can also be used in other similar application scenarios. A root-knot nematode infection observation device is described in detail below.

[0033] Example 1

[0034] See attached Figure 1 — Figure 3 As shown, a schematic diagram of the structure of a preferred embodiment of a root-knot nematode infection observation device of the present application is shown. The root-knot nematode infection observation device comprises a tray structure 1, the tray structure 1 comprises evenly arranged card slots 11, and an observation tube 2 for planting rice is evenly arranged on the tray structure 1 through the card slots 11. The outer wall of the observation tube 2 is provided with a scale line 3 for measuring the length of the rice root system, wherein the observation tube is made of transparent materials such as glass and polystyrene; it should be particularly noted that the graduation value of the scale line 3 is 1 mm.

[0035] The utility model provides an installation position for the observation tube 2 through the setting of the card slot 11; through the setting of the observation tube 2, combined with the setting that the observation tube is made of a transparent material, the rice is planted in the observation tube 2, and the early infection process of the root-knot nematode can be directly observed, ensuring that the experimental results are accurate and reliable, and combined with the setting of the scale line, the length of the rice root system can be directly measured, without the need to pull out the rice plant for cleaning, thereby protecting the rice root system, ensuring the accuracy of the test data, and the length of the rice root system can be measured more quickly and efficiently, reducing the work intensity of the experimenter. It should be particularly noted that the 0 scale line in the scale line 3 is located on the top plane of the overhead layer 21.

[0036] See attached Figure 2 and Figure 3 As shown, in the utility model, an overhead layer 21 is provided at the bottom of the observation tube 2, and the height of the overhead layer 21 is greater than the depth of the slot 11; wherein a sponge plug 22 is installed at the pipe mouth of the observation tube 2, an observation plane 23 is provided on the outer wall of the observation tube 2, and a fluid replenishment channel 5 is also provided on the observation tube 2.

[0037] The utility model ensures that the bottom surface of the inner cavity of the observation tube 2 is higher than the top surface of the tray 12 by setting the overhead layer 21 and limiting the height of the overhead layer 21, so that the entire inner cavity of the observation tube 2 can be observed, thereby observing the rice; by setting the sponge plug 22, the pipe mouth of the observation tube 2 can be blocked to keep the observation tube 2 moist, thereby reducing the loss of water in the culture matrix and allowing the plant to exchange gas with the external environment; by setting the observation plane 23, the outer wall of the observation tube 2 is non-circular, so that after the observation tube 2 is snap-fitted with the slot 11, the observation tube 2 will not rotate at will, ensuring that the position of the observation tube 2 will not move at will.

[0038] See attached Figure 3 As shown, in the utility model, the fluid infusion channel 5 includes a fluid infusion port 51, a fluid outlet 52 provided on the observation tube 2 and connected to the inner cavity of the observation tube 2, and a connecting channel 53 provided on the observation tube 2 for connecting the fluid infusion port 51 and the fluid outlet 52; wherein the fluid infusion port 51 is located on the observation plane 23, and is arranged at a position close to the tube mouth of the observation tube 2; the distance between the lowest point of the fluid outlet 52 and the bottom surface of the inner cavity is not less than 8 cm. The utility model can supplement nutrient solution by setting the liquid replenishing port 51, and there is no need to remove the sponge plug 22 before supplementing the nutrient solution, so that the operation is convenient and the observation tube 2 can be prevented from being damaged by collision or loosening of the sponge plug 22 and the observation tube 22 during the removal of the sponge plug 22; by limiting the height of the liquid outlet 52, the gel used for rice planting is avoided to prevent the gel from blocking the liquid outlet 52; it should be particularly noted that the gel is Pluronic F-127 melt; the connecting channel 53 can be a connecting hole or a connecting groove; the number of liquid outlets 52 is not less than 1.

[0039] See attached Figure 1 As shown, in the present invention, the tray structure 1 further includes a tray 12 , and the card slots 11 are evenly arranged on the tray 12 .

[0040] See attached Figure 1 — Figure 3 As shown, the use process of the utility model is as follows:

[0041] First, a Pluronic F-127 solution is prepared in a beaker, and then the prepared solution is added into the observation tube 2, and a single rice seedling is inserted into the Pluronic F-127 solution. Subsequently, a certain number of Poaceae root-knot nematodes are introduced into the observation tube 2, and the number of root knots of rice is observed and counted at certain intervals, and the root length is observed through the scale line 3.

[0042] Example 2

[0043] See attached Figure 4 — Figure 6 As shown, the difference between this embodiment and embodiment 1 lies in the specific structure of the tray structure 1, which is as follows:

[0044] The pallet structure 1 of this embodiment includes a pallet base 13, a plurality of moving plates 14 movably mounted on the pallet base 13 at equal intervals, and the card slots 11 are arranged on the moving plates 14 at equal intervals; the pallet base 13 is provided with a slide groove 15 corresponding to the moving plates 14, and a card connection structure 4 is arranged between the slide groove 15 and the moving plates 14. It should be particularly noted that the straight line formed by the moving track of the moving plate 14 is parallel to the observation plane 23; the cross section of the moving plate 14 is T-shaped.

[0045] In this embodiment, the observation tubes 2 are divided into multiple groups through the setting of the movable plate 14, that is, the observation tubes 2 on each movable plate 14 are one group, and the observation tubes 2 connected to the movable plate 14 can be driven to move by the movement of the movable plate 14, so that the staff can observe the observation tube 2 located in the middle position and replenish the nutrient solution for the observation tube 2 in the middle position; through the setting of the slide groove 15, an installation position is provided for the movable plate 14, and the movement of the movable plate 14 is guided; through the setting of the clamping structure 4, a detachable connection is made between the tray base 13 and the movable plate 14, and the movable plate 14 can be moved along the slide groove 15 when necessary, or it can be fixed on the tray base 13, so that when the tray base 13 is transported or taken, the movable plate 14 can be prevented from moving and causing damage to the observation tube 2.

[0046] See attached Figure 4 — Figure 6As shown, in the utility model, a mounting groove 131 is provided at the bottom of the tray base 13, and a connecting groove 132 is provided in the mounting groove 131; a movable groove 141 is provided on the top surface of the movable plate 14, and a limiting groove 142 corresponding to the connecting groove 132 is provided in the movable groove 141; a clamping structure 4 is arranged in the mounting groove 131, the connecting groove 132, the movable groove 141 and the limiting groove 142; wherein, the clamping structure 4 includes a movable block 41 movably installed in the mounting groove 131, a clamping block 42 installed on the movable block 41 and adapted to the connecting groove 132 and made of a magnetic conductive material, a telescopic spring 43 installed in the movable groove 141, a telescopic block 44 installed in the movable groove 141, and a magnet 45 installed on the telescopic block 44 and adapted to the limiting groove 142.

[0047] The utility model provides an installation position for the movable block 41 by setting the installation groove 131; provides an installation position for the clamping block 42 by setting the connecting groove 132; provides an installation position for the telescopic spring 43 of the telescopic block 44 by setting the movable groove 141; provides a connection position for the magnet 45 and the clamping block 42 by setting the limiting groove 142; it should be particularly noted that the size of the limiting groove 142 is larger than the size of the clamping block 42, and the two are interference fit; the setting of the telescopic spring 43 provides spring power so that the telescopic block 44 can be telescopically moved; the setting of the magnet 45, in conjunction with the facility that the clamping block 42 is made of magnetic conductive material, a magnetic force is generated between the clamping block 42 and the magnet 45. It should be particularly noted that the magnetic force can be greater than the gravity between the clamping block 42 and the movable block 41, so that the clamping block 42 and the movable block 41 can be moved, so that the clamping block 42 is inserted into the limiting groove 142.

[0048] See attached Figure 5 and Figure 6 As shown, in the utility model, the size of the installation groove 131 is larger than the size of the connecting groove 132; the size of the movable groove 141 is larger than the size of the limiting groove 142; the telescopic block 44 is provided with an abutment block 441 in contact with the telescopic spring 43 away from the magnet 45, and the abutment block 441 is adapted to the movable groove 141; a baffle 143 is installed at the notch of the movable groove 141 away from the limiting groove 142; a sealing plate 133 is installed at the notch of the installation groove 131 away from the connecting groove 132. It should be particularly noted that the size of the telescopic block 44 is not larger than the size of the limiting groove 142, that is, the telescopic block 44 can move in the limiting groove 142; when the bottom surface of the moving block 41 contacts the sealing plate 133, the clamping block 42 is separated from the limiting groove 142; the compression distance of the telescopic spring 43 is greater than the stroke of the moving block 41 in the installation groove 131, and the elastic force of the telescopic spring 43 is sufficient to push the telescopic block 44, the abutment block 441 and the magnet 45 to move, and the size of the clamping block 42 is smaller than the size of the limiting groove 142.

[0049] The utility model sets the abutment block 441 so that the telescopic block 44 can contact the telescopic spring 43. When the telescopic block 44 is pressed, the telescopic spring 43 can be squeezed. When released, the telescopic spring 43 can provide a reset force to push the telescopic block 44 to reset. The abutment block 441 is limited by the baffle plate 143 to prevent the telescopic block 44 from separating from the movable groove 141. The sealing plate 133 is set to limit the movable block 41, limit the moving range of the movable block 41, and prevent the movable block 41 from separating from the mounting groove 131.

[0050] See attached Figure 4 — Figure 6 As shown, the specific operation process of the tray structure 1 in this embodiment is as follows:

[0051] When the movable plate 14 needs to be installed into the slide groove 15, it is only necessary to align the movable plate 14 with the slide groove 15 and insert it. When the movable plate 14 moves to the specified position, that is, when the movable plate 14 moves to the end of the slide groove 15, the limiting groove 142 on the movable plate 14 is connected with the connecting groove 132, and the magnetic force between the magnet 45 and the clamping block 42 overcomes the gravity of the clamping block 42 and the movable block 41 and the friction between the clamping block 42 and the movable block 41 and the groove wall, so that the clamping block 42 and the movable block 41 move upward, and the clamping block 42 is inserted into the limiting groove 142 to limit the movable plate 14.

[0052] When the movable plate 14 needs to be disassembled, the staff only needs to press the telescopic block 44. At this time, the telescopic block 44 drives the abutment block 441 to move downward to squeeze the telescopic spring 43. When the telescopic spring 43 is compressed, the telescopic block 44 drives the magnet 45 to move downward to a position flush with the bottom surface of the movable plate 14. The movable block 41 is blocked by the sealing plate 133 and cannot move further. At this time, the clamping block 42 is separated from the limiting groove 142, and the staff only needs to pull the movable plate 14. The operation is simple and convenient.

[0053] The above embodiments are intended to illustrate the present application, not to limit the present application. Any solution that is a simple transformation of the present application falls within the protection scope of the present application.

Claims

1. A root-knot nematode infection observation device, characterized in that: The invention comprises a tray structure (1), the tray structure (1) comprising evenly arranged card slots (11), observation tubes (2) for planting rice evenly arranged on the tray structure (1) through the card slots (11), the outer wall of the observation tube (2) being provided with scale lines (3) for measuring the length of the rice root system, and the observation tube (2) being made of a transparent material.

2. The root-knot nematode infection observation device according to claim 1, characterized in that: An overhead layer (21) is provided at the bottom of the observation tube (2), and the height of the overhead layer (21) is greater than the depth of the slot (11).

3. The root-knot nematode infection observation device according to claim 2, characterized in that: A sponge plug (22) is installed at the tube mouth of the observation tube (2), an observation plane (23) is provided on the outer wall of the observation tube (2), and a fluid replenishment channel (5) is also provided on the observation tube (2).

4. The root-knot nematode infection observation device according to claim 3, characterized in that: The fluid infusion channel (5) comprises a fluid infusion port (51), a fluid outlet (52) provided on the observation tube (2) and connected to the inner cavity of the observation tube (2), and a communication channel (53) provided on the observation tube (2) and used for connecting the fluid infusion port (51) and the fluid outlet (52).

5. The root-knot nematode infection observation device according to claim 4, characterized in that: The liquid replenishing port (51) is located on the observation plane (23) and is disposed at a position close to the pipe opening of the observation pipe (2); The distance between the lowest point of the liquid outlet (52) and the bottom surface of the inner cavity is not less than 8 cm.

6. The root-knot nematode infection observation device according to claim 1, characterized in that: The tray structure (1) further comprises a tray (12), and the card slots (11) are evenly arranged on the tray (12).

7. The root-knot nematode infection observation device according to claim 1, characterized in that: The pallet structure (1) further comprises a pallet base (13), a plurality of movable plates (14) movably mounted on the pallet base (13) at equal intervals, the clamping grooves (11) being arranged on the movable plates (14) at equal intervals; a slide groove (15) corresponding to the movable plates (14) is provided on the pallet base (13), and a clamping structure (4) is provided between the slide groove (15) and the movable plates (14).

8. The root-knot nematode infection observation device according to claim 7, characterized in that: The bottom of the tray base (13) is provided with a mounting groove (131), and a connecting groove (132) is provided in the mounting groove (131); a movable groove (141) is provided on the top surface of the movable plate (14), and a limiting groove (142) corresponding to the connecting groove (132) is provided in the movable groove (141); and the clamping structure (4) is arranged in the mounting groove (131), the connecting groove (132), the movable groove (141) and the limiting groove (142).

9. The root-knot nematode infection observation device according to claim 8, characterized in that: The clamping structure (4) comprises a moving block (41) movably mounted in the mounting groove (131), a clamping block (42) mounted on the moving block (41) and adapted to the connecting groove (132), a telescopic spring (43) mounted in the movable groove (141), a telescopic block (44) mounted in the movable groove (141), and a magnet (45) mounted on the telescopic block (44) and adapted to the limiting groove (142).

10. The root-knot nematode infection observation device according to claim 9, characterized in that: The size of the installation groove (131) is larger than the size of the connecting groove (132); the size of the movable groove (141) is larger than the size of the limiting groove (142); An abutment block (441) in contact with the telescopic spring (43) is provided on the telescopic block (44) away from the magnet (45), and the abutment block (441) is matched with the movable groove (141); a baffle (143) is installed at the notch of the movable groove (141) away from the limiting groove (142); A sealing plate (133) is installed at the slot opening of the installation slot (131) away from the connecting slot (132).

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