A special hematopoietic stem cell freezing box

Abstract

The utility model discloses a special hematopoietic stem cell freezing box, relates to the field of stem cell freezing, solves the problem that a conventional stem cell freezing box cannot set a frozen sample in a single space in a freezing tank, which will cause the freezing bag to rupture, and when the sample volume is large in the later stage, it is necessary to search for the sample for many times, which prolongs the searching time of the staff, increases the possibility of medical staff being frostbitten by liquid nitrogen, and has low safety. The utility model comprises a box body, a lower box body is arranged at the bottom of the box body, a middle box body is arranged in the middle of the box body, an upper box body is arranged at the top of the box body, two support blocks are arranged at the top of the box body, the two support blocks are arranged symmetrically, two upper slide rails are arranged symmetrically, the upper slide rails are slidably connected to the box body, the middle right side of the upper box body, the middle box body and the lower box body are all provided with a lower slide rail, and a pull ring is arranged inside the support block.

Description

Technical Field

[0001] The utility model relates to the technical field of stem cell cryopreservation, in particular to a special hematopoietic stem cell cryopreservation box. Background Art

[0002] Hematopoietic stem cells are adult stem cells in the blood system. They are a heterogeneous group with the ability to self-renew for a long time and the potential to differentiate into various mature blood cells. They are the type of adult stem cells with the longest and most in-depth research history, and have important guiding significance for the study of various stem cells, including tumor stem cells.

[0003] Most leukemias, especially acute myeloid leukemia (AML) and chronic myeloid leukemia (CML), are directly or indirectly related to abnormalities in hematopoietic stem cells. CML is a common type of leukemia in which the most classic chromosomal translocation leads to the malignant transformation of hematopoietic stem cells. Most other acute myeloid leukemias are directly derived from the malignant transformation of progenitor cells. Hematopoietic stem cells first acquire major pathogenic mutations such as chromosomal translocations, but this does not affect their ability to differentiate into mature cells with normal functions. When the hematopoietic stem cells with chromosomal translocations or their differentiated downstream cells receive a second blow, leukemia will be induced.

[0004] Hematopoietic stem cells also play a certain role in regulating the microenvironment of solid tumors. For example, prostate tumor cells can simulate the molecular signals of hematopoietic stem cells, enter the hematopoietic microenvironment, and cause changes in the expression spectrum of hematopoietic stem cells, forcing hematopoietic stem cells to leave. They can also leave the hematopoietic microenvironment by expressing molecules related to hematopoietic cell migration, ultimately leading to tumor metastasis.

[0005] In clinical treatment, hematopoietic stem cell transplantation is widely used in the treatment of blood system diseases and autoimmune diseases. In the treatment of other solid tumors, such as lymphoma, germ cell tumor, breast cancer, and small cell lung cancer, it is mainly used in patients who have failed conventional treatment or relapsed and are refractory to treatment and have poor prognostic factors.

[0006] Plasma is the liquid part remaining after removing blood cells from whole blood. It is an important component of blood. The main components of plasma include water, various inorganic ions such as potassium ions, calcium ions, sodium ions, various coagulation factors, fibrinogen, various antibodies, albumin, etc. Plasma has many important functions: Maintaining the colloidal osmotic pressure of plasma: This is mainly achieved through albumin, which absorbs water in the blood vessels to prevent water from penetrating outside the blood vessels. If the plasma albumin concentration decreases, the colloidal osmotic pressure decreases, and tissue edema will occur; Hemostatic effect: Plasma is rich in coagulation factors, which plays a very important role in the body's hemostatic function. When the concentration of coagulation factors decreases, the body will experience bleeding symptoms; Enhance immunity: Plasma contains a large amount of globulin, which can significantly increase the body's immunity; Transport function: Plasma can transport substances needed to maintain human life activities, and transport nutrients, carbon dioxide, oxygen and other substances in the body to various organs of the body through the blood system, so that various organs and tissues of the body can function normally; Maintaining balance: Plasma contains more water, protein, sugar, lipids and other substances, which can maintain fluid balance and promote metabolism. Plasma is an important component for maintaining normal physiological functions of the human body and has important physiological significance.

[0007] In the traditional cell storage process, most of the time, when storing hematopoietic stem cells, only the hematopoietic stem cells are stored, and the sample test tubes and the hematopoietic stem cells frozen in the hematopoietic stem cell bags are placed separately. This makes it difficult to find them in the later search process. In addition, once a special sample leaks due to special reasons, it may also affect other samples and thus may affect the use of the remaining stem cells. The cryopreservation box designed in the present invention can store hematopoietic stem cells and plasma from the same source at the same time. Plasma contains rich nutrients and corresponding proteins in the blood. Most companies will specially treat the remaining plasma after extraction. The cryopreservation box of the present invention can completely preserve the plasma for later use, and can effectively ensure the integrity of the plasma components to avoid the loss of components or special treatment of the plasma during special treatment.

[0008] At present, most samples are stored separately in a corresponding cryopreservation bag, and then packaged in a cryopreservation box. All hematopoietic stem cells are placed in the same relatively open liquid nitrogen tank according to their numbers. The frozen samples cannot be set up in a separate space in the cryopreservation tank, which will cause the cryopreservation bag to rupture. In the later stage, when the number of samples is large, it is necessary to search for samples many times, which prolongs the staff's search time and increases the possibility of medical staff being frostbitten by liquid nitrogen, which is less safe.

[0009] Therefore, the utility model provides a special hematopoietic stem cell freezing box to solve the above problems. Utility Model Content

[0010] In view of the above situation, in order to overcome the defects of the prior art, the utility model provides a special hematopoietic stem cell freezing box. Through this design, it is effectively solved that the conventional stem cell freezing box cannot set up a separate space for the frozen samples in the freezing tank, which will cause the freezing bag to rupture, and when the sample volume is large in the later stage, it is necessary to search for the samples many times, which prolongs the search time of the staff, increases the possibility of medical staff being frostbitten by liquid nitrogen, and has low safety.

[0011] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: the utility model includes a box body, a lower box body is provided at the bottom of the box body, a middle box body is provided in the middle of the box body, an upper box body is provided at the top of the box body, two support blocks are provided at the top of the box body, and the two support blocks are symmetrically arranged on the left and right, two upper slide rails are provided on the top of the upper box body, the middle box body and the lower box body, and the two upper slide rails are symmetrically arranged on the left and right, the upper slide rails are slidably connected to the box body, and a lower slide rail is provided on the right side of the middle of the upper box body, the middle box body and the lower box body, a pull ring is provided inside the support block, a locking assembly is provided on the right side of the pull ring, and a plurality of blocking plates are provided on the rear side of the locking assembly.

[0012] Preferably, the rear sides of the upper box body, the middle box body and the lower box body are all provided with cylindrical blocks, and the middle part of the upper slide rail is provided with a plurality of rollers.

[0013] Preferably, both the middle box body and the lower box body are provided with cavities inside.

[0014] Preferably, a plurality of grooves are provided inside the upper box body.

[0015] Preferably, the locking assembly comprises a cylindrical rod, the cylindrical rod is connected to the right side of the pull ring, a connecting rod is provided on the right side of the cylindrical rod, and a pin is provided on the right side of the bottom of the connecting rod.

[0016] Preferably, a limit rod is provided on the outer side of the pin shaft, a slider is provided at the bottom of the limit rod, the slider is connected to the right side of the box body, a cross bar is provided on the rear side of the bottom of the slider, a vertical bar is provided on the rear side of the cross bar, and the vertical bar and the blocking plate are connected to each other.

[0017] The beneficial effects of the utility model are:

[0018] 1. The utility model can set up a separate space for frozen samples in the cryopreservation tank, which can effectively prevent samples in the same liquid nitrogen tank from being contaminated by other samples due to special reasons (cryostorage bags rupture, freezing bags are damaged due to taking and placing, and the bags are relatively fragile in low-temperature conditions), and can effectively ensure the safety of samples, effectively save space, and maximize the space utilization rate. In addition, the provision of sample cryopreservation tubes can effectively avoid sampling from the cryopreservation bags and reduce the risk of contamination during use. The layered design can effectively improve the space utilization rate and facilitate rapid identification of samples. The placement of cryopreservation bags in partitions can facilitate the screening and search of samples. The lying cryopreservation tubes can also effectively reduce the space height and improve the space utilization rate.

[0019] 2. Since all samples and test samples are in the same freezing box, when the sample volume is large in the later stage, multiple searches can be effectively avoided, which greatly saves the staff's time and effectively reduces the possibility of frostbite by liquid nitrogen, thereby improving the safety of the staff.

[0020] 3. Through the cooperation of the locking assembly, the blocking plate and the lower rail of the upper slide rail, the movement of the upper box body, the middle box body and the lower box body can be effectively restricted to ensure the safety of the upper box body, the middle box body and the lower box body when storing, taking out and replacing. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the overall structure of the utility model.

[0022] Figure 2 It is a schematic diagram of the planar structure of the utility model.

[0023] Figure 3 For this utility model Figure 3 A partial enlarged schematic diagram of part A in the middle.

[0024] Figure 4 It is a schematic diagram of the planar structure of the upper box body of the utility model.

[0025] Figure 5 It is a schematic diagram of the matching structure of the connecting rod and the blocking plate of the utility model.

[0026] Figure 6 This is a schematic diagram of the pull ring of the utility model in a folded state.

[0027] Figure 7 It is a schematic diagram of the inclined state of the pull ring of the utility model.

[0028] Figure 8 For this utility model Figure 7 A partial enlarged schematic diagram of part A in the middle.

[0029] Numbers in the figure: 1. box body; 2. pull ring; 3. support block; 4. cylindrical rod; 5. upper box body; 6. middle box body; 7. lower box body; 8. upper slide rail; 9. roller; 10. lower slide rail; 11. groove; 12. pin shaft; 13. limit rod; 14. slider; 15. cross bar; 16. vertical bar; 17. blocking plate; 18. connecting rod. DETAILED DESCRIPTION

[0030] The following will refer to the attached Figures 1 to 8 The embodiments of the present invention are described in detail. It should be understood by those skilled in the art that these embodiments are only used to explain the technical principle of the present invention and are not intended to limit the protection scope of the present invention.

[0031] Embodiment 1:

[0032] Please see attached Figures 1 to 8 A special hematopoietic stem cell freezing box: comprising a box body 1, the box body 1 is 8 cm in height, 15 cm in length, and 8 cm in width, a lower box body 7 is provided at the bottom of the box body 1, a middle box body 6 is provided in the middle of the box body 1, and an upper box body 5 is provided on the top of the box body 1. The upper box body 5, the lower box body 7, and the middle box body 6 can all be manually pushed and pulled inside the box body 1 to take out and put in the freezing bags or freezing tubes, and two support blocks 3 are provided on the top of the box body 1, and the two support blocks 3 are arranged symmetrically on the left and right, and the two support blocks 3 are fixed, and the tops of the upper box body 5, the middle box body 6, and the lower box body 7 Two upper slide rails 8 are provided, and the two upper slide rails 8 are arranged symmetrically on the left and right. The slide rails on the top of the upper box body 5, the middle box body 6, and the lower box body 7 can support the upper box body 5, the middle box body 6, and the lower box body 7, and constrain the upper box body 5, the middle box body 6, and the lower box body 7 to only move horizontally forward and backward. The lower slide rail 10 is also slidably connected to the side wall of the box body 1. A pull ring 2 is provided inside the support block 3. The pull ring 2 becomes a vertical state when in use, which is convenient for taking the box body 1 out of liquid nitrogen. When not in use, it is rotated and folded horizontally on the top of the box body 1 to reduce space occupation.

[0033] The rear sides of the upper box body 5, the middle box body 6 and the lower box body 7 are all provided with cylindrical blocks, and the opening and closing states of the upper box body 5, the middle box body 6 and the lower box body 7 can be controlled by pulling the cylindrical blocks. A plurality of rollers 9 are provided in the middle of the upper slide rail 8, and the plurality of rollers 9 inside the upper slide rail 8 can reduce the friction force when the upper box body 5, the middle box body 6 and the lower box body 7 move.

[0034] Both the middle box body 6 and the lower box body 7 are provided with cavities inside, and the inside of the cavities is used to accommodate the freezing bags.

[0035] The interior of the upper box body 5 is provided with a plurality of grooves 11, and the number of the grooves 11 is 6, so that the cryotubes can be laid flat, thereby effectively reducing the space height and improving the space utilization of the device.

[0036] All parts in this freezing box are made of PC (high-strength polycarbonate) and can be frozen in liquid nitrogen.

[0037] This product is designed to store 3 cryopreservation bags and 6 cryopreservation tubes. Among the three cryopreservation bags, one can effectively preserve hematopoietic stem cells, one can effectively preserve plasma after sample separation, and one can effectively preserve red blood cells remaining after separation. After separation, the sample is basically divided into plasma layer, buffy coat layer, and red blood cell layer. Too many positions for cryopreservation bags not only waste space but also have no actual preservation value. As for the positions for cryopreservation tubes, 2 tubes of plasma, 2 tubes of hematopoietic stem cells, and 2 tubes of red blood cells can be placed. When preparing for use, the sample in one of the tubes can be subjected to some microbial and safety tests, while the other tubes can be subjected to some special tests to avoid repeated extraction from the cryopreservation bag, avoid repeated freezing and thawing, reduce cell quality problems, and better ensure cell viability. Similarly, since all samples from the same source are in the same storage space, corresponding test tubes can also be used to perform sample safety tests.

[0038] The utility model can set up a separate space for the frozen samples in the freezing tank, which can effectively prevent the samples in the same liquid nitrogen tank from being contaminated by other samples due to special reasons (cryostorage bags rupture, freezing bags are damaged due to taking and placing, and the bags are relatively fragile in the low temperature state), and can effectively ensure the safety of the samples.

[0039] Since all samples and test samples are in the same freezing box, when the sample volume is large in the later stage, multiple searches can be effectively avoided, which greatly saves the staff's time and effectively reduces the possibility of frostbite by liquid nitrogen, thereby improving the safety of the staff.

[0040] Embodiment 2:

[0041] On the basis of Example 1, considering that when the device is in use, the box body 1 may shake during the process of taking and placing the cryobox, thereby causing the upper box body 5, the middle box body 6, and the lower box body 7 to move, causing the cryobags and cryotubes stored inside to fall, thereby causing damage to the samples.

[0042] The upper slide rail 8 is slidably connected to the box body 1, and the upper box body 5, the middle box body 6, and the lower box body 7 are all provided with a lower slide rail 10 on the right side of the middle part. The blocking plate 17 moves upward to block the upper slide rail 8, and moves downward to block the lower slide rail 10, thereby realizing the locking of the upper box body 5, the middle box body 6, and the lower box body 7. A locking assembly is provided on the right side of the pull ring 2, and a plurality of blocking plates 17 are provided on the rear side of the locking assembly. The locking assembly includes a cylindrical rod 4, which is connected to the right side of the pull ring 2, and the cylindrical rod 4 can rotate with the rotation of the pull ring 2. A connecting rod 18 is provided on the right side of the cylindrical rod 4, and a pin shaft 12 is provided on the right side of the bottom of the connecting rod 18. The rotation of the cylindrical rod 4 can drive the fixedly connected connecting rod 18 to rotate, and the connecting rod 18 drives the pin shaft 12 fixedly connected at the bottom to move in a circle.

[0043] A limit rod 13 is provided on the outer side of the pin shaft 12. When the pin shaft 12 moves in a circle, the limit rod 13 connected in a sliding manner can be driven to move. A slider 14 is provided at the bottom of the limit rod 13. The slider 14 is connected to the right side of the box body 1. The slider 14 is slidably connected to the box body 1. A slide groove is provided on the right side of the box body 1, which can constrain the slider 14 to move vertically. A cross bar 15 is provided on the rear side of the bottom of the slider 14. A vertical bar 16 is provided on the rear side of the cross bar 15. The vertical bar 16 and the blocking plate 17 are connected to each other. When the slider 14 moves upward, the cross bar 15 and the vertical bar 16 will be driven to move upward at the same time, thereby driving multiple blocking plates 17 to change in height.

[0044] When the utility model is used, when the box body 1 is stored, the pull ring 2 will automatically fold into a horizontal state under the action of gravity, driving the cylindrical rod 4 to rotate, and the pin shaft 12 can drive the slidably connected limit rod 13 to move when the circumference moves. A slider 14 is provided at the bottom of the limit rod 13, and a slide groove is provided on the right side of the box body 1, which can constrain the slider 14 to move only vertically. When the slider 14 moves upward, it will drive the cross bar 15 and the vertical bar 16 to move upward at the same time, thereby driving the multiple blocking plates 17 to move upward, so as to achieve the following effect. Figure 6In the state shown, the right side of the upper slide rail 8 at the upper end of the upper box body 5, the middle box body 6, and the lower box body 7 is blocked to prevent the upper box body 5, the middle box body 6, and the lower box body 7 from sliding during storage. When the box body 1 needs to be placed in liquid nitrogen for storage, the pull ring 2 is first rotated to a vertical state, the pull ring 2 is hung by a hook, and the box body 1 is placed in the liquid nitrogen. During the rotation of the pull ring 2, the three blocking plates 17 are driven to move downward to block the lower slide rails 10 of the upper box body 5, the middle box body 6, and the lower box body 7. The blocking plates 17 are used to prevent the upper box body 5, the middle box body 6, and the lower box body 7 from sliding out due to shaking during the storage process of the box body 1. In addition, if the box body 1 shakes during the storage process, the three blocking plates 17 will be driven to rise and fall accordingly. The blocking plates 17 block a large area of ​​the lower rail 10 and the upper rail 8. Although the blocking plates 17 will rise and fall slightly, they will not cause the upper box body 5, the middle box body 6, and the lower box body 7 to slide out and fall. When it is necessary to take out the sample in the box body 1, the box body 1 is first placed on the desktop, and then the box body 1 is placed on the desktop. Figure 6 The pull ring 2 in the state shown is rotated to Figure 7 In the state shown, the pull ring 2 rotates 45 degrees, driving the blocking plate 17 to move between the upper slide rail 8 and the lower slide rail 10, and no longer blocks the upper slide rail 8 and the lower slide rail 10. Then the upper box body 5, the middle box body 6, and the lower box body 7 can be opened manually, which is very convenient and safe.

[0045] It should be noted that in the description of the present invention, the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" and the like indicating directions or positional relationships are based on the directions or positional relationships shown in the drawings, which are only for the convenience of description, and do not indicate or imply that the device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0046] In addition, it should be noted that in the description of the present invention, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For those skilled in the art, the specific meanings of the above terms in the present invention can be understood according to the specific circumstances.

[0047] So far, the technical solution of the present invention has been described in conjunction with the preferred embodiments shown in the accompanying drawings. However, it is easy for those skilled in the art to understand that the protection scope of the present invention is obviously not limited to these specific embodiments. Without departing from the principle of the present invention, those skilled in the art can make equivalent changes or substitutions to the relevant technical features, and the technical solutions after these changes or substitutions will fall within the protection scope of the present invention.

Claims

1. A special hematopoietic stem cell freezing box, characterized in that: The utility model comprises a box body (1), wherein a lower box body (7) is provided at the bottom of the box body (1), a middle box body (6) is provided in the middle of the box body (1), an upper box body (5) is provided at the top of the box body (1), two support blocks (3) are provided at the top of the box body (1), and the two support blocks (3) are symmetrically arranged on the left and right, two upper slide rails (8) are provided at the top of the upper box body (5), the middle box body (6) and the lower box body (7), and the two upper slide rails (8) are symmetrically arranged on the left and right, and the upper slide rails (8) are slidably connected to the box body (1), and a lower slide rail (10) is provided on the right side of the middle of the upper box body (5), the middle box body (6) and the lower box body (7), a pull ring (2) is provided inside the support block (3), a locking assembly is provided on the right side of the pull ring (2), and a plurality of blocking plates (17) are provided on the rear side of the locking assembly.

2. A dedicated hematopoietic stem cell freezing box according to claim 1, characterized in that: The rear sides of the upper box body (5), the middle box body (6) and the lower box body (7) are all provided with cylindrical blocks, and the middle part of the upper slide rail (8) is provided with a plurality of rollers (9).

3. The dedicated hematopoietic stem cell freezing box according to claim 1, characterized in that: The middle box body (6) and the lower box body (7) are both provided with cavities inside.

4. The dedicated hematopoietic stem cell freezing box according to claim 1, characterized in that: A plurality of grooves (11) are provided inside the upper box body (5).

5. The dedicated hematopoietic stem cell freezing box according to claim 1, characterized in that: The locking assembly comprises a cylindrical rod (4), wherein the cylindrical rod (4) is connected to the right side of the pull ring (2), a connecting rod (18) is provided on the right side of the cylindrical rod (4), and a pin shaft (12) is provided on the right side of the bottom of the connecting rod (18).

6. A dedicated hematopoietic stem cell freezing box according to claim 5, characterized in that: A limiting rod (13) is provided on the outer side of the pin shaft (12), a slider (14) is provided at the bottom of the limiting rod (13), the slider (14) is connected to the right side of the box body (1), a cross bar (15) is provided at the rear side of the bottom of the slider (14), a vertical rod (16) is provided at the rear side of the cross bar (15), and the vertical rod (16) and the blocking plate (17) are connected to each other.

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