Biotoxin nucleic acid probe pretreatment device

Abstract

The utility model relates to the technical field of rapid food safety detection, and specifically to a biological toxin nucleic acid probe pretreatment device, including an L-shaped support seat, a fixed box is fixedly installed on the side wall of the support seat, and the box mouth of the fixed box faces downward, a placement box is slidably installed on the support seat, a test tube rack is slidably installed on the placement box, and an adjustment mechanism is arranged inside the placement box. When the magnetic beads in the separation column need to be oscillated, the utility model first places the bottom end of the magnetic rod sleeve in the separation column through a cylinder, and the adjustment column slides in the adjustment groove on the limiter through a motor and an adjustment rod. The adjustment column slides between the arc-shaped part and the non-arc-shaped part of the adjustment groove so that the magnetic rod sleeve moves up and down in the separation column, completing the oscillation of the magnetic rod sleeve on the magnetic beads in the separation column, and the magnetic beads are dispersed in the separation column, and react evenly with the reagents in the separation column, so that the reaction is more complete, thereby avoiding the situation where uneven oscillation affects the experimental results.

Description

Technical Field

[0001] The utility model relates to the technical field of rapid food safety detection, in particular to a biological toxin nucleic acid probe pre-processing device. Background Art

[0002] Rapid food safety testing requires the rapid separation and purification of target biological toxins in complex samples. Traditional immunoaffinity columns based on the principle of antibody immune recognition are expensive due to the high cost of antibodies, limited regeneration capacity and number of times, etc., which results in high testing costs in food testing and cannot be used on a large scale.

[0003] Using nucleic acid aptamers as recognition molecules to construct sample pretreatment purification columns for target biological toxins can greatly reduce the sample pretreatment and detection costs during toxin detection. At the same time, fixing the composite nucleic acid aptamers on the surface of magnetic beads can simultaneously perform pretreatment enrichment and purification on two common aflatoxins B1 and M1. The magnetic properties of the magnetic beads can also be used to release the magnetic field after enrichment, which is more conducive to the regeneration of the enriched materials and realize multiple recycling, further reducing the detection cost and improving the utilization efficiency.

[0004] The nucleic acid recognition probes of two biological toxins, aflatoxin B1 and M1, are coupled to the surface of magnetic beads and loaded into columns. Sample pretreatment and separation and purification of the target toxins are carried out under the action of an external magnetic field. After elution is completed, the external magnetic field can be removed and the nucleic acid probes on the surface of the magnetic beads can be washed and regenerated for reuse.

[0005] Among them, when the target toxin on the magnetic beads in the sample is separated and purified by a magnet, whether the nucleic acid probe is attached to the magnetic beads or the magnetic beads are enriched and cleaned, the magnetic rod cover needs to be oscillated multiple times to make the magnetic beads shake in different reagents, disperse the magnetic beads, and allow the magnetic beads and the samples attached to the magnetic beads to react completely with the reagents. Usually this step is performed manually. Manual oscillation of the magnetic rod cover may result in the magnetic beads not reacting completely with the reagents, which may affect the experimental results and increase the loss of experimental supplies. Utility Model Content

[0006] In view of the above shortcomings of the prior art, the utility model provides a biotoxin nucleic acid probe pretreatment device, which can effectively solve the problem in the prior art that the magnetic beads and reagents do not react sufficiently when the magnetic rod cover is manually oscillated, resulting in poor experimental results.

[0007] In order to achieve the above objectives, the present invention is implemented through the following technical solutions:

[0008] The utility model provides a biotoxin nucleic acid probe pre-processing device, comprising an L-shaped support seat, a fixed box is fixedly installed on the side wall of the support seat, and the box mouth of the fixed box faces downward, a placement box is slidably installed on the support seat, a test tube rack is slidably installed on the placement box, an adjustment mechanism is arranged inside the placement box, the adjustment mechanism is transmission-connected with the test tube rack, a magnetic rack is arranged on the support seat, a motor is fixedly installed on the side wall of the support seat, the motor is placed in the fixed box, a rotating shaft is fixedly installed on the output end of the motor, an adjustment rod is fixedly installed on the end of the rotating shaft away from the motor, an adjustment column is fixedly installed on the end of the adjusting rod away from the rotating shaft, a limiting piece is slidably connected on the inner wall of the fixed box, the limiting piece is composed of a large semicircular ring plate and a horizontal plate fixed up and down, the axis of the rotating shaft is the same as that of the large semicircular ring plate, an adjustment groove slidably connected with the adjustment column is opened on the side of the limiting piece close to the motor, a connecting rod is fixedly installed on the bottom side of the limiting piece, and a magnetic rod sleeve is arranged below the connecting rod.

[0009] Preferably, a fixing rod is fixedly installed at the bottom end of the connecting rod, connecting frames are symmetrically fixedly installed at both ends of the fixing rod, T-shaped limit rods are fixedly installed at both ends of the top side of the magnetic rod sleeve, and the bottom ends of the two connecting frames are provided with limit grooves which are respectively connected to the two limit rods for limiting sliding.

[0010] Preferably, two fixing blocks are symmetrically fixedly mounted on the upper curved surface of the limiting member, springs are fixedly mounted on the upper ends of the two fixing blocks, and the upper ends of the two springs are fixedly connected to the inner top wall of the fixing box.

[0011] Preferably, the adjustment mechanism comprises a cylinder fixedly mounted on the inner bottom wall of the placement box, a support plate is fixedly mounted on the telescopic end of the cylinder, and the test tube rack is placed on the upper end of the support plate.

[0012] Preferably, stabilizing blocks are symmetrically fixedly mounted on both sides of the support plate, and a stabilizing groove which is limitedly slidably connected to the two stabilizing blocks is formed on the inner wall of the fixing box.

[0013] Preferably, the upper end of the support seat is provided with a sliding groove which is limitedly slidably connected to the placement box.

[0014] Compared with the known prior art, the technical solution provided by the utility model has the following beneficial effects:

[0015] When the utility model needs to oscillate the magnetic beads in the separation column, the cylinder is first used to place the bottom end of the magnetic rod sleeve in the separation column, and the motor drives the adjusting rod to rotate through the rotating shaft. When the adjusting rod rotates, the adjusting column on the adjusting rod also slides in the adjusting groove. When the adjusting column slides from the arcuate part of the limiting member to the non-arculose part in the adjusting groove, the adjusting column squeezes the limiting member, so that the limiting member and the connecting rod move downward, and when the adjusting column slides from the non-arculose part into the arcuate part, the limiting member and the connecting rod move upward again, and then the magnetic rod sleeve is moved up and down in the separation column through the connecting rod, so that the magnetic rod sleeve oscillates the magnetic beads in the separation column, the magnetic beads are dispersed in the separation column, react evenly with the reagent in the separation column, and the reaction is more complete, thereby avoiding the situation where uneven oscillation affects the experimental results. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the prior art descriptions are briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model, and for ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0017] Figure 1 It is a three-dimensional structural schematic diagram of the utility model;

[0018] Figure 2 It is a schematic diagram of the side cross-sectional structure of the fixing box of the utility model;

[0019] Figure 3 This is a schematic diagram of the structure of the motor, the limiter and the connecting rod of the utility model;

[0020] Figure 4 It is a structural schematic diagram of the fixing rod, the connecting frame and the magnetic rod sleeve of the utility model;

[0021] Figure 5 It is a schematic diagram of the main cross-sectional structure of the placement box of the utility model.

[0022] Figure numerals: 1. support base; 2. fixing box; 3. placement box; 4. test tube rack; 5. magnetic rack; 6. motor; 7. rotating shaft; 8. adjusting rod; 9. adjusting column; 10. limiting piece; 11. adjusting slot; 12. connecting rod; 13. fixing rod; 14. connecting frame; 15. magnetic rod sleeve; 16. fixing block; 17. spring; 18. limiting rod; 19. limiting slot; 20. cylinder; 21. support plate; 22. stabilizing block; 23. stabilizing slot; 24. slide slot. DETAILED DESCRIPTION

[0023] In order to make the purpose, technical solution and advantages of the embodiment of the utility model clearer, the technical solution in the embodiment of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiment of the utility model. Obviously, the described embodiment is a part of the embodiment of the utility model, not all of the embodiments. Based on the embodiment of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0024] The utility model is further described below in conjunction with embodiments.

[0025] Example: Refer to Figures 1 to 5The biological toxin nucleic acid probe pretreatment device comprises an L-shaped support seat 1, a fixed box 2 is fixedly installed on the side wall of the support seat 1, and the box mouth of the fixed box 2 faces downward, a placement box 3 is slidably installed on the support seat 1, a test tube rack 4 is slidably installed on the placement box 3, an adjustment mechanism is arranged inside the placement box 3, and the adjustment mechanism is transmission-connected with the test tube rack 4, a magnetic rack 5 is arranged on the support seat 1, a motor 6 is fixedly installed on the side wall of the support seat 1, the motor 6 is placed in the fixed box 2, a rotating shaft 7 is fixedly installed on the output end of the motor 6, an adjusting rod 8 is fixedly installed on the end of the rotating shaft 7 away from the motor 6, an adjusting column 9 is fixedly installed on the end of the adjusting rod 8 away from the rotating shaft 7, a limiting member 10 is slidably connected to the inner wall of the fixed box 2, and the limiting member 10 It is composed of a large semicircular ring plate and a horizontal plate fixed up and down, the rotating shaft 7 is the same as the axis of the large semicircular ring plate, and an adjusting groove 11 slidably connected to the adjusting column 9 is opened on the side of the limiting member 10 close to the motor 6, and a connecting rod 12 is fixedly installed on the bottom side of the limiting member 10, and a magnetic rod sleeve 15 is arranged below the connecting rod 12; in the experiment, the separation column loaded with magnetic beads is placed on the test tube rack 4, and the test tube rack 4 is raised to a suitable position through the adjustment mechanism. At this time, the bottom end of the magnetic rod sleeve 15 is placed in the separation column, and the motor 6 is started. The output end of the motor 6 drives the adjusting rod 8 to rotate through the rotating shaft 7. When the adjusting rod 8 rotates, the adjusting column 9 on the adjusting rod 8 also slides in the adjusting groove 11, and the adjusting column 9 moves from the arc portion of the limiting member 10 in the adjusting groove 11. When sliding to the non-arc-shaped part, the adjusting column 9 squeezes the limiter 10, so that the limiter 10 and the connecting rod 12 move downward, and when the adjusting column 9 slides from the non-arc-shaped part into the arc-shaped part, the limiter 10 and the connecting rod 12 move upward again, and then the magnetic rod sleeve 15 moves up and down in the separation column through the connecting rod 12, completing the oscillation of the magnetic rod sleeve 15 on the magnetic beads in the separation column. After the reaction of the magnetic beads in the separation column is completed, the motor 6 is turned off, and the magnetic frame 5 is placed in the magnetic rod sleeve 15. Under the action of the magnetic frame 5, the magnetic beads in the separation column are enriched on the outside of the magnetic rod sleeve 15, and the test tube rack 4 is lowered by the adjusting mechanism to separate the magnetic beads from the separation column, and the separation column on the test tube rack 4 is replaced with a separation column containing reagents required for the next reaction. , and then the test tube rack 4 is moved up by the adjustment mechanism. After the magnetic beads are placed in the separation column on the test tube rack 4, the magnetic rack 5 is removed from the magnetic rod sleeve 15, and the magnetic beads are no longer enriched on the magnetic rod sleeve 15. The oscillation process driven by the motor 6 is repeated to allow the magnetic beads and the reagent to react fully. The above operation process is then repeated many times until the magnetic beads complete multiple operation steps such as enrichment and cleaning. After the separation and purification of the target toxin in the sample is completed, the obtained reagents are collected, and then the nucleic acid probes on the surface of the magnetic beads are cleaned and regenerated by the oscillator. The motor 6, the adjustment column 9 and the adjustment slot 11 are used to disperse the magnetic beads in the separation column, and react evenly with the reagents in the separation column. The reaction is more complete, avoiding the situation where uneven oscillation affects the experimental results.

[0026] Furthermore, a fixing rod 13 is fixedly installed at the bottom end of the connecting rod 12, and connecting frames 14 are symmetrically fixedly installed at both ends of the fixing rod 13. T-shaped limit rods 18 are fixedly installed at both ends of the top side of the magnetic rod sleeve 15, and the bottom ends of the two connecting frames 14 are provided with limit grooves 19 which are respectively connected to the two limit rods 18 for limiting sliding; the connecting rod 12 is connected to the magnetic rod sleeve 15 through the fixing rod 13 and the connecting frame 14, and the magnetic rod sleeve 15 is slidably connected to the connecting frame 14 through the limit rod 18 and the limit groove 19. When it is necessary to take the magnetic frame 5, first slide the magnetic rod sleeve 15 and the limit rod 18 out of the limit groove 19, and then take the magnetic frame 5 to prevent the magnetic frame 5 from being blocked by the fixing rod 13 and the connecting frame 14 when installing it on the magnetic rod sleeve 15.

[0027] Furthermore, two fixed blocks 16 are symmetrically fixedly installed on the upper curved surface of the limit member 10, and springs 17 are fixedly installed on the upper ends of the two fixed blocks 16. The other ends of the two springs 17 are fixedly connected to the inner top wall of the fixed box 2; the elastic force of the spring 17 supports the limit member 10 to prevent the limit member 10 from falling downward due to gravity, so as to ensure that the adjustment column 9 can slide normally in the adjustment groove 11.

[0028] Specifically, the adjustment mechanism includes a cylinder 20 fixedly mounted on the inner bottom wall of the placement box 3, a support plate 21 is fixedly mounted on the telescopic end of the cylinder 20, and the test tube rack 4 is placed on the upper end of the support plate 21; when the test tube rack 4 needs to be raised or lowered, the cylinder 20 is started, and the output end of the cylinder 20 drives the test tube rack 4 to move up and down through the support plate 21.

[0029] Furthermore, stabilizing blocks 22 are symmetrically fixedly installed on both sides of the support plate 21, and a stabilizing groove 23 is opened on the inner wall of the fixed box 2 to be limitedly slidably connected to the two stabilizing blocks 22; when the support plate 21 moves up and down, the stabilizing blocks 22 at both ends of the support plate 21 also slide in the stabilizing groove 23, and the stabilizing blocks 22 and the stabilizing groove 23 make the support plate 21 move up and down more smoothly, preventing the test tube rack 4 from tilting.

[0030] Furthermore, a slide groove 24 is provided at the upper end of the support seat 1 and is limitedly slidably connected to the placement box 3; when the separation column on the test tube rack 4 needs to be replaced, the placement box 3 can be slid in the slide groove 24 so that the placement box 3 is no longer placed directly below the magnetic rod sleeve 15, which is convenient for the operator to replace the separation column.

[0031] The working principle of this device is as follows:

[0032] In the experiment, the separation column loaded with magnetic beads is placed on the test tube rack 4, and the cylinder 20 is started. The output end of the cylinder 20 causes the test tube rack 4 to rise to a suitable position through the support plate 21. At this time, the bottom end of the magnetic rod sleeve 15 is placed in the separation column, and the motor 6 is started. The output end of the motor 6 drives the adjustment rod 8 to rotate through the rotating shaft 7. When the adjustment rod 8 rotates, the adjustment column 9 on the adjustment rod 8 also slides in the adjustment groove 11. When the adjustment column 9 slides from the arc portion of the limiter 10 to the non-arc portion in the adjustment groove 11, the adjustment column 9 is pressed against the limiter. When the adjusting column 9 slides from the non-arc-shaped portion into the arc-shaped portion, the limiting member 10 and the connecting rod 12 move upward again, and then the magnetic rod sleeve 15 moves up and down in the separation column through the connecting rod 12, so that the magnetic rod sleeve 15 oscillates the magnetic beads in the separation column. After the reaction of the magnetic beads in the separation column is completed, the motor 6 is turned off, the magnetic rod sleeve 15 and the placement box 3 are slid to the end away from the connecting frame 14, and then the magnetic frame 5 is placed in the magnetic rod sleeve 15. Under the action of the rack 5, the magnetic beads in the separation column are all enriched on the outside of the magnetic rod sleeve 15, the test tube rack 4 is lowered by the cylinder 20 to separate the magnetic beads from the separation column, and the separation column on the test tube rack 4 is replaced with a separation column containing reagents required for the next reaction, and the test tube rack 4 is moved up by the cylinder 20, and after the magnetic beads are placed in the separation column on the test tube rack 4, the magnetic rack 5 is removed from the magnetic rod sleeve 15, and the magnetic beads are no longer enriched on the magnetic rod sleeve 15, and the magnetic rod sleeve 15 and the placement box 3 are slid back to the bottom of the connecting rack 14, and the motor 6 is driven to repeat the process. The magnetic beads are oscillated in a dynamic manner to allow the magnetic beads to react fully with the reagents, and the above-mentioned operation process is then repeated many times until the magnetic beads have completed multiple operation steps such as enrichment and cleaning. After the target toxin in the sample is separated and purified, the obtained reagents are collected, and then the nucleic acid probes on the surface of the magnetic beads are cleaned and regenerated by a rotary oscillator. The magnetic beads are dispersed in the separation column through the motor 6, the adjustment column 9 and the adjustment slot 11, and react evenly with the reagents in the separation column to make the reaction more complete, thereby avoiding the situation where uneven oscillation affects the experimental results.

[0033] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some of the technical features therein by equivalents. These modifications or replacements will not cause the essence of the corresponding technical solutions to deviate from the protection scope of the technical solutions of the embodiments of the present invention.

Claims

1. A biotoxin nucleic acid probe pretreatment device, characterized in that: The invention comprises an L-shaped support base (1), a fixed box (2) is fixedly mounted on the side wall of the support base (1), and the box opening of the fixed box (2) faces downwards; a placement box (3) is slidably mounted on the support base (1), a test tube rack (4) is slidably mounted on the placement box (3), an adjustment mechanism is arranged inside the placement box (3), and the adjustment mechanism is transmission-connected with the test tube rack (4); a magnetic rack (5) is arranged on the support base (1), a motor (6) is fixedly mounted on the side wall of the support base (1), the motor (6) is placed in the fixed box (2), a rotating shaft (7) is fixedly mounted on the output end of the motor (6), and the rotating shaft (7) 7) An adjusting rod (8) is fixedly installed at one end away from the motor (6), and an adjusting column (9) is fixedly installed at one end of the adjusting rod (8) away from the rotating shaft (7). A limiting member (10) is slidably connected to the inner wall of the fixed box (2). The limiting member (10) is composed of a large semicircular ring plate and a horizontal plate fixed up and down. The axis of the rotating shaft (7) is the same as that of the large semicircular ring plate. An adjusting groove (11) slidably connected to the adjusting column (9) is provided on the side of the limiting member (10) close to the motor (6). A connecting rod (12) is fixedly installed on the bottom side of the limiting member (10), and a magnetic rod sleeve (15) is arranged below the connecting rod (12).

2. The biotoxin nucleic acid probe pretreatment device according to claim 1, characterized in that: A fixing rod (13) is fixedly mounted on the bottom end of the connecting rod (12), connecting frames (14) are symmetrically fixedly mounted on both ends of the fixing rod (13), T-shaped limiting rods (18) are fixedly mounted on both ends of the top side of the magnetic rod sleeve (15), and limiting grooves (19) are respectively slidably connected to the two limiting rods (18) at the bottom ends of the two connecting frames (14).

3. The biotoxin nucleic acid probe pretreatment device according to claim 1, characterized in that: Two fixing blocks (16) are symmetrically fixedly mounted on the upper curved surface of the limiting member (10), springs (17) are fixedly mounted on the upper ends of the two fixing blocks (16), and the upper ends of the two springs (17) are fixedly connected to the inner top wall of the fixing box (2).

4. The biotoxin nucleic acid probe pretreatment device according to claim 1, characterized in that: The adjustment mechanism comprises a cylinder (20) fixedly mounted on the inner bottom wall of the placement box (3); a support plate (21) is fixedly mounted on the telescopic end of the cylinder (20); and the test tube rack (4) is placed on the upper end of the support plate (21).

5. The biotoxin nucleic acid probe pretreatment device according to claim 4, characterized in that: Stabilizing blocks (22) are symmetrically fixedly mounted on both sides of the support plate (21), and a stabilizing groove (23) is provided on the inner wall of the fixing box (2) and is limitedly slidably connected to the two stabilizing blocks (22).

6. The biotoxin nucleic acid probe pretreatment device according to claim 1, characterized in that: The upper end of the support seat (1) is provided with a sliding groove (24) which is limitedly slidably connected to the placement box (3).

Images