A cell culture bottle

Abstract

The utility model relates to the technical field of cell culture, and discloses a cell culture bottle, comprising a culture bottle, wherein a bottom wall is arranged at the bottom of the culture bottle, a liquid-blocking and breathable membrane is arranged at the opening of the culture bottle, a closing cover is installed on the outside of the culture bottle, a fixing ring is fixedly connected to the outside of the culture bottle, a plug is arranged inside the fixing ring, and a sealing block is fixedly connected to the inside of the plug. The cell culture bottle has the advantages of being convenient for adding culture fluid and reducing the influence of the external environment on the internal environment of the culture bottle. The plug is used to close a pipeline, and the culture bottle is divided into two cavities by a partition plate, so that culture fluid can be added through the pipeline, and a blocking mechanism is opened by gravity to add culture fluid to the inside of the cavity for culturing cells, so as to complete the addition of culture fluid, thereby solving the problem that the external environment may contaminate cells when the closing cover is opened to pour the culture fluid through the opening during the current cell culture process.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a cell culture bottle. Background Art

[0002] The in vitro culture of cells and tissues has become an indispensable part of life research and practice. There are many types of cells that can be cultured, from viruses to bacteria and fungi, from human cells to animal cells and plant cells. Some cells and tissues can grow in suspension, but a considerable number of mammalian cells need to adhere to the surface. Cell culture flasks are used for cell culture. Under the conditions of limited manpower, material resources and space, a large amount of experimental data on basic cell growth information and culture process parameters can be obtained. Therefore, cell culture flasks are widely used in laboratory research and industrial production.

[0003] The culture bottles currently used include a bottle body, a bottle cap, a liquid-resistant and breathable membrane and other structures to achieve cell culture. However, during the cell culture process, the bottle cap needs to be opened repeatedly during the process of inoculating cells, replenishing culture medium and harvesting cells. In particular, the culture fluid needs to be added in batches. There are multiple opening operations of the culture bottle during the inoculation, replenishment and harvesting processes, which can easily lead to the problem of cells in culture being contaminated by external bacteria and viruses. For this reason, we propose a cell culture bottle. Utility Model Content

[0004] In view of the disadvantage that in the prior art, when adding culture fluid during cell culture, it is necessary to open the closed cover and pour the culture fluid through the opening, which may cause the external environment to contaminate the cells, the utility model provides a cell culture bottle, which has the advantages of facilitating the addition of culture fluid and reducing the impact of the external environment on the internal environment of the culture bottle.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a cell culture bottle, comprising a culture bottle, a bottom wall is arranged at the bottom of the culture bottle, a liquid-blocking and breathable membrane is arranged at the opening of the culture bottle, a closing cover is installed on the outside of the culture bottle, a fixing ring is fixedly connected to the outside of the culture bottle, a plug is arranged inside the fixing ring, a sealing block is fixedly connected to the inside of the plug, and a pipeline is arranged inside the fixing ring.

[0006] Preferably, a partition plate is fixedly connected to the interior of the culture bottle, a side wall is arranged outside the partition plate, a liquid outlet is arranged at the tail end of the pipeline, and a blocking mechanism is installed inside the partition plate.

[0007] Preferably, the blocking mechanism comprises a fixed shell, a sealing ball is arranged inside the fixed shell, and a spring is fixedly connected to the bottom of the sealing ball.

[0008] Preferably, a through hole is provided inside the closing cover for ventilation.

[0009] Preferably, the distance between the bottom wall and the pipeline is greater than the distance between the top outer wall of the culture bottle and the pipeline, the pipeline is fixedly connected to the inside of the culture bottle, and the sealing block is clamped to the inside of the pipeline.

[0010] Preferably, the plane of the side of the side wall close to the fixing ring is lower than the plane of the side of the side wall away from the fixing ring, which is used for drainage and can drive the culture fluid to flow to the blocking mechanism under the action of gravity when the culture bottle is tilted.

[0011] Preferably, the liquid outlet is configured as a pointed cone-shaped opening, the inclined surface of which is disposed close to the inner wall of the culture bottle, which can reduce the possibility of bubbles appearing during the addition of culture fluid.

[0012] Preferably, a groove body corresponding to the sealing ball is opened inside the fixed shell, the length of the groove body is greater than the length of the sealing ball, and the sealing ball can move inside the groove body.

[0013] Beneficial effects:

[0014] 1. The cell culture bottle is implanted with cells in the culture bottle by opening the closed cover and taking out the liquid-blocking and breathable membrane, and then the liquid-blocking and breathable membrane and the closed cover are installed to culture the cells. When culture fluid needs to be added, the plug is pulled out, and culture fluid is added to the inside of the pipe through the sterile pipe. The culture fluid enters the inside of the culture bottle through the liquid outlet, and then the culture bottle is tilted to make the culture fluid flow to the blocking mechanism. Under the action of gravity, the sealing ball squeezes the spring to deform it, so as to open the blocking mechanism to make the culture fluid flow to the area where the cells are located. The setting of the pipe facilitates the addition of culture fluid and reduces the opening size when adding culture fluid. The matching sterile pipe can be used to add culture fluid, thereby reducing the influence of the external environment on the internal environment of the culture bottle.

[0015] 2. The cell culture bottle reduces the probability of bubble generation by means of an inclined liquid outlet. The liquid outlet is arranged close to the inner wall of the culture bottle, which can effectively reduce the collision of the culture solution with the inner wall of the culture bottle during the process of injecting the culture solution into the culture bottle, thereby reducing the possibility of bubble generation. The plug is clamped inside the fixing ring, and the sealing block is clamped inside the pipeline, which can increase the sealing performance of the device. The structure is simple and easy to use. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the structure of the utility model;

[0017] Figure 2 This is a schematic diagram of the structure of the fixing ring of the utility model;

[0018] Figure 3 This is a schematic diagram of the structure of the partition board of the utility model;

[0019] Figure 4It is a schematic diagram of the cross-sectional structure of the fixed shell of the utility model.

[0020] In the figure: 1. culture bottle; 2. bottom wall; 3. liquid-blocking and breathable membrane; 4. closing cover; 5. fixing ring; 6. pipeline; 7. plug; 8. sealing block; 9. partition plate; 10. side wall; 11. liquid outlet; 12. blocking mechanism; 13. fixed shell; 14. sealing ball; 15. spring. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] Embodiment 1

[0023] See also Figure 1-4 A cell culture bottle comprises a culture bottle 1, wherein a bottom wall 2 is arranged at the bottom of the culture bottle 1, a liquid-blocking and breathable membrane 3 is arranged at the opening of the culture bottle 1, a closing cover 4 is installed on the outside of the culture bottle 1, a fixing ring 5 is fixedly connected to the outside of the culture bottle 1, a pipe 6 is arranged inside the fixing ring 5, a partition plate 9 is fixedly connected to the inside of the culture bottle 1, a side wall 10 is arranged outside the partition plate 9, a liquid outlet 11 is arranged at the tail end of the pipe 6, a blocking mechanism 12 is installed inside the partition plate 9, a through hole is opened inside the closing cover 4 for ventilation, a distance between the bottom wall 2 and the pipe 6 is greater than a distance between the top outer wall of the culture bottle 1 and the pipe 6, the pipe 6 is fixedly connected to the inside of the culture bottle 1, a sealing block 8 is clamped inside the pipe 6, a plane on the side of the side wall 10 close to the fixing ring 5 is lower than a plane on the side of the side away from the fixing ring 5, and is used for drainage, and can drive the culture fluid to flow to the blocking mechanism 12 under the action of gravity when the culture bottle 1 is tilted.

[0024] Among them, the liquid outlet 11 is set as a pointed cone opening, and its inclined surface is set close to the inner wall of the culture bottle 1, which can reduce the possibility of bubbles appearing during the addition of culture liquid. A groove body corresponding to the sealing ball 14 is opened inside the fixed shell 13, and the length of the groove body is greater than the length of the sealing ball 14, and the sealing ball 14 can move inside the groove body.

[0025] Working principle: When the cell culture bottle is in use, the liquid-blocking and breathable membrane 3 can be taken out by opening the closing cover 4 to implant cells into the culture bottle 1, and then the liquid-blocking and breathable membrane 3 and the closing cover 4 can be installed to culture the cells. When it is necessary to add culture fluid, the plug 7 is pulled out, and the culture fluid is added to the inside of the pipe 6 through the sterile pipe. The culture fluid enters the inside of the culture bottle 1 through the liquid outlet 11, and then the culture bottle 1 is tilted to allow the culture fluid to flow to the blocking mechanism 12. Under the action of gravity, the sealing ball 14 squeezes the spring 15 to deform it, so as to open the blocking mechanism 12 and allow the culture fluid to flow to the area where the cells are located. The setting of the pipe 6 facilitates the addition of culture fluid and reduces the size of the opening when adding culture fluid. The matching sterile pipe can be used to add culture fluid, thereby reducing the impact of the external environment on the internal environment of the culture bottle.

[0026] Embodiment 2

[0027] See also Figure 1-4 Further on the basis of the first embodiment, a plug 7 is arranged inside the fixing ring 5, a sealing block 8 is fixedly connected inside the plug 7, the blocking mechanism 12 includes a fixed shell 13, a sealing ball 14 is arranged inside the fixed shell 13, and a spring 15 is fixedly connected to the bottom of the sealing ball 14.

[0028] Working principle: When the cell culture bottle is in use, the probability of bubble generation can be reduced by setting the liquid outlet 11 obliquely. The liquid outlet 11 is set close to the inner wall of the culture bottle 1, which can effectively reduce the collision of the culture liquid with the inner wall of the culture bottle 1 during the process of injecting the culture liquid into the culture bottle 1, thereby reducing the possibility of bubble generation. The plug 7 is clamped inside the fixing ring 5, and the sealing block 8 is clamped inside the pipe 6, which can increase the sealing performance of the device. The structure is simple and easy to use.

[0029] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cell culture bottle, comprising a culture bottle (1), characterized in that: The bottom of the culture bottle (1) is provided with a bottom wall (2), the opening of the culture bottle (1) is provided with a liquid-blocking and gas-permeable membrane (3), the outside of the culture bottle (1) is provided with a closing cover (4), the outside of the culture bottle (1) is fixedly connected with a fixing ring (5), the inside of the fixing ring (5) is provided with a plug (7), the inside of the plug (7) is fixedly connected with a sealing block (8), and the inside of the fixing ring (5) is provided with a pipeline (6); the inside of the culture bottle (1) is fixedly connected with a partition plate (9), the outside of the partition plate (9) is provided with a side wall (10), the tail end of the pipeline (6) is provided with a liquid outlet (11), and the inside of the partition plate (9) is provided with a blocking mechanism (12).

2. A cell culture bottle according to claim 1, characterized in that: The blocking mechanism (12) comprises a fixed shell (13), a sealing ball (14) is arranged inside the fixed shell (13), and a spring (15) is fixedly connected to the bottom of the sealing ball (14).

3. A cell culture bottle according to claim 1, characterized in that: A through hole is provided inside the closing cover (4) for ventilation.

4. A cell culture bottle according to claim 1, characterized in that: The distance between the bottom wall (2) and the pipe (6) is greater than the distance between the top outer wall of the culture bottle (1) and the pipe (6); the pipe (6) is fixedly connected to the inside of the culture bottle (1); and the sealing block (8) is snap-fitted to the inside of the pipe (6).

5. A cell culture bottle according to claim 1, characterized in that: The plane of the side of the side wall (10) close to the fixing ring (5) is lower than the plane of the side of the side wall (10) away from the fixing ring (5), and is used for drainage.

6. A cell culture bottle according to claim 1, characterized in that: The liquid outlet (11) is configured as a pointed cone-shaped opening, the inclined surface of which is disposed close to the inner wall of the culture bottle (1).

7. A cell culture bottle according to claim 2, characterized in that: A groove body corresponding to the sealing ball (14) is provided inside the fixed housing (13), and the length of the groove body is greater than the length of the sealing ball (14).

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