A multi-stage screening system for highly active targeted microbial strains
By designing a multi-stage screening system with plug-in connections and a hook ring rod structure, the problems of inconvenient operation of microbial strain screening devices and difficulty in collecting screens in the prior art are solved, and efficient and convenient strain screening and processing processes are achieved.
Patent Information
- Application Number
- CN202421368594.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-17
- Publication Date
- 2025-04-08
- Estimated Expiration
- 2034-06-17
AI Technical Summary
In the prior art, the microbial strain screening device is inconvenient to operate and the screening connection method is complicated, which makes it difficult to clean and replace, and it is difficult to easily collect and transfer mycorrhizal fungi on the bottom screen.
A multi-stage screening system for high-active targeted microbial strains is designed, using a plug-in screening box structure, allowing the screening box to rotate quickly, clean and replace, and the convenient collection and transfer of the bottom screen through hook and ring rod structures.
It realizes convenient operation of the screening process, reduces manual intervention, improves the efficiency of screen cleaning and replacement, and ensures that the mycorrhizal fungi on the bottom screen can be quickly and conveniently collected and processed.
Smart Images
Figure CN222724774U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of microbial strain screening, in particular to a multi-stage screening system for high-activity targeted microbial strains. Background Art
[0002] Highly active targeted microbial strains refer to those with specific functions that can improve soil quality, such as rhizobia, mycorrhizal fungi, or microorganisms in bio-organic waste. These microbial strains are usually obtained through screening.
[0003] In the prior art, mycorrhizal fungi are generally screened by a microbial strain screening device, which includes multiple sieve bodies arranged up and down, each sieve body is provided with a sieve with different mesh sizes, the upper sieve intercepts large particles of impurities in the soil, and the bottom sieve intercepts mycorrhizal fungi spores, and finally the mycorrhizal fungi on the sieve are transferred and cleaned. However, the prior art requires the soil and mycorrhizal fungi to be separated by holding the handle on the sieve body, which is inconvenient to operate; and the multiple sieve bodies of the prior art are connected by means of threads and buckles. Each time the sieve is cleaned or replaced, the sieve bodies need to be disassembled one by one, which is inconvenient. At the same time, it is not convenient to collect and transfer the mycorrhizal fungi on the bottom sieve. Utility Model Content
[0004] In order to solve the technical problems existing in the above-mentioned background technology, the utility model provides a multi-stage screening system for highly active targeted microbial strains.
[0005] The technical solution of this utility model is as follows:
[0006] A high-activity targeted microbial strain multi-stage screening system comprises a base placed on a reference surface, a plurality of screening frames are arranged from bottom to top on the base, each screening frame is correspondingly provided with a screen, the apertures of the plurality of screens decrease from top to bottom, the bottom screening frame is hung on the base, and a distance is left between the bottom screening frame and the upper end surface of the base;
[0007] Adjacent screening frames are plug-connected, a single screening frame has an insertion rod on its upper end face, a slot corresponding to the insertion rod on its lower end face, and the screening frame above the lowest screening frame can rotate around the insertion rod; the insertion rod includes a first insertion rod and a second insertion rod vertically arranged on the upper end face of the screening frame, and the length of the first insertion rod is longer than that of the second insertion rod.
[0008] By setting the connection mode of the upper and lower adjacent screening frames to a simple plug-in connection, it is convenient to rotate the screening frame to quickly clean or replace the screen thereon, and the corresponding screening frame can be directly pulled out for separate cleaning and replacement, so that the mycorrhizal fungi intercepted by the lower screening frame can be washed out into the corresponding strain container for the next step of centrifugal separation.
[0009] To prevent the screening frame from rotating during use, the specific structure of the plug rod is set so that the first plug rod is 1-2 times longer than the second plug rod. The corresponding screening frame needs to be pushed upward to a height higher than the second plug rod before the screening frame can be pushed out or rotated to a certain angle.
[0010] Regarding the arrangement positions of the first insertion rod and the second insertion rod, the first insertion rod and the second insertion rod are symmetrically arranged on both sides of the screening frame.
[0011] In order to unify the rotation positions of the screening frames, the first insertion rods on the multiple screening frames are coaxially arranged so that the first insertion rods are located on the same side, thereby facilitating the simultaneous rotation of several adjacent screening frames.
[0012] Regarding the aperture of the sieve in the bottom screening frame, the aperture selection range is 55μm-100μm, which can screen mycorrhizal fungi within this size range.
[0013] To facilitate the collection of the mixture of soil and clean water, a collecting container located below the screening frame is provided on the upper end surface of the base. Compared with the existing technology, the lowest screen and the sewage flowing through the lowest screen can be separated by a certain distance to prevent the sewage from contaminating the lowest screen.
[0014] In order to prevent the screening frame from being interfered by the bottom screen when rotating along the first insertion rod, the lower end surface of the screen is 0.5-2 cm higher than the lower end surface of the screening frame.
[0015] Regarding the specific structure of the base, the base includes a bottom plate and a support frame arranged on one side of the bottom plate, and the outer side of the lowest screening frame is hung on the support frame.
[0016] The specific structure of the above-mentioned support frame is that the support frame includes a vertical rod and an annular rod fixed as one, the lower end of the vertical rod is connected to the base, and the upper end is connected to the horizontally arranged annular rod, and the lowest screening frame is hung on the annular rod by a hook. The hook can drive the lowest screening frame to rotate along the circumferential direction of the annular rod, so as to facilitate shaking the mixture of soil samples and clean water on multiple screens, so that there is no liquid residue on multiple screens at different heights.
[0017] The beneficial effects of the utility model are:
[0018] After the soil sample with mycorrhizal fungi is mixed with water, it is poured onto the top sieve and filtered through multiple layers of sieves. Large particles or impurities in the soil are intercepted by the upper sieves, and the bottom sieve can intercept most of the mycorrhizal fungi. Then the bottom sieve is disassembled for collection, and the remaining material is centrifuged by sucrose centrifugation method, and then filtered to obtain the mycorrhizal fungi strain sample. This operation is completed on the base, and there is no need for the staff to hold the handle to operate;
[0019] The connection mode of adjacent screening frames is plug-in connection, and the screen is installed at the bottom of the screening frame. When the screen needs to be replaced or cleaned, the corresponding screening frame is directly rotated at a certain angle to make it misaligned with the adjacent screening frame below. The rotated screen can be directly cleaned with clean water, and the screen at the bottom of the screening frame can be quickly replaced.
[0020] You can also directly pull the filter frame upwards to clean or replace it separately. Cleaning and replacement are simple and fast.
[0021] When the mycorrhizal fungi intercepted on the bottom screen need to be collected, the upper screening frame is quickly pulled out, and the screening net is removed from the support frame of the base, and the strains on the screen can be washed into the corresponding strain container for the next step of centrifugal separation;
[0022] The first insertion rod is 1-2 times longer than the second insertion rod. When the mixture of soil sample and clean water is poured into the top screen, multiple screening frames are moved up and down, and the height of the up and down movement is less than the height of the second insertion rod, so that the liquid on the multiple screens can flow downward, so that no liquid remains on each screen.
[0023] When the mixture of soil sample and water flows downward from the top sieve, the flow rate is slow and may accumulate on one side to cause blockage, affecting the downward flow of the mixture. For this reason, the bottom screening frame is hung on the annular rod of the base through a hook and can rotate on the annular rod. In conjunction with the above-mentioned operation of moving the screening frame up and down, the bottom screening frame is rotated to drive the mixture on multiple sieves to flow quickly, thereby improving the separation efficiency. BRIEF DESCRIPTION OF THE DRAWINGS
[0024] In the attached picture:
[0025] Figure 1 It is a top view;
[0026] Figure 2 for Figure 1 AA section structural diagram;
[0027] Figure 3 for Figure 2 The enlarged structural diagram at A in the middle;
[0028] Figure 4 It is a schematic diagram of the secondary screening frame structure;
[0029] Figure 5 This is a schematic diagram of the first-level filter box and the second-level filter box after rotation;
[0030] The components represented by the reference numerals in the figure are:
[0031] 1. First-level screening frame; 2. Second-level screening frame; 3. Third-level screening frame; 4. First plug-in rod; 5. Second plug-in rod; 6. Slot; 7. First-level screen; 8. Second-level screen; 9. Third-level screen; 10. Base; 101. Bottom plate; 102. Vertical rod; 103. Ring rod; 11. Collecting container; 12. Hook. DETAILED DESCRIPTION
[0032] See also Figure 1 and Figure 2 A multi-stage screening system for highly active targeted microbial strains is shown, comprising a base 10 placed on a reference plane, and a plurality of screening frames are arranged above the base 10 from bottom to top. In this embodiment, three screening frames are arranged, and the three screening frames are respectively a primary screening frame 1, a secondary screening frame 2 and a tertiary screening frame 3 from top to bottom. Each screening frame is provided with a corresponding screen, and the three screens are respectively a primary screen 7, a secondary screen 8 and a tertiary screen 9 from top to bottom. The apertures of the primary screen 7, the secondary screen 8 and the tertiary screen 9 decrease from top to bottom, forming a multi-stage screening system for filtering the mixture of soil samples and clean water step by step until the mycorrhizal fungi are intercepted on the lowest screen, and then the mycorrhizal fungi on the screen are washed into the corresponding strain container, and then the remaining material is centrifuged by sucrose centrifugation method, and then filtered to obtain the mycorrhizal fungus strain sample.
[0033] The upper primary screen 7 is used to intercept gravel or impurities with larger diameters, the secondary screen 8 is used to filter particles or impurities with smaller diameters, and the aperture selection range of the lower tertiary screen 9 is 55μm-100μm. The aperture size is selected according to the soil sample or actual conditions so that it can intercept most of the mycorrhizal fungi and screen the mycorrhizal fungi within this size range. The remaining sewage can flow downward from the tertiary screen 9 to the collection vessel 11.
[0034] See also Figure 2 and Figure 5As shown, adjacent screening frames are plug-connected. The upper end faces of the secondary screening frame 2 and the tertiary screening frame 3 of this embodiment are respectively provided with plug rods. If a screening frame is also provided above the primary screening frame 1, a plug rod is also provided on the upper end face of the primary screening frame 1, and slots 6 corresponding to the plug rods are provided on the lower end faces of the three screening frames. In order to prevent the primary screening frame 1, the secondary screening frame 2 and the tertiary screening frame 3 from rotating when in use, the specific structure of the plug rod is provided as follows: the plug rod includes a first plug rod 4 and a second plug rod 5 provided on the upper end face of the corresponding screening frame, the first plug rod 4 and the second plug rod 5 are symmetrically provided on both sides of the screening frame, and the first plug rod 4 is 1-2 times longer than the second plug rod 5. The corresponding screening frame needs to be pushed upward to a height higher than the second plug rod 5 in order to push the screening frame out or rotate it to a certain angle, so as to quickly clean or replace the screen on it. Specifically, when the second plug rod 5 is separated from the corresponding slot, and the first plug rod 4 is not separated from its corresponding slot, the primary screening frame 1 and the secondary screening frame 2 can respectively rotate with the corresponding first plug rod 4 as the center.
[0035] See also Figure 4 As shown, the length of the first insertion rod 4 is set to be twice the length of the second insertion rod 5. In this embodiment, when the mixture of soil sample and clean water is poured into the first-level screen 7, multiple screening frames are moved up and down, and the height of the up and down movement is less than the height of the second insertion rod 5, which is convenient for the liquid on the multiple screens to flow downward, so that there is no liquid residue on each screen, which has the effect of filtering the mixture or mycorrhizal fungi. There is no liquid residue on the third-level screen 9, which is convenient for cleaning and collecting the mycorrhizal fungi.
[0036] In order to unify the rotation position of the screening frames, the first plug rods 4 on the multiple screening frames are coaxially arranged so that the first plug rods 4 are located on the same side, which is convenient for rotating several adjacent screening frames at the same time. In order to prevent the primary screening frame 1, the secondary screening frame 2 and the tertiary screening frame 3 from being interfered by the corresponding screen at the bottom when rotating along the first plug rod 4, thereby affecting their rotation after moving up, the above-mentioned screen is installed at the position height of the corresponding screening frame, and the lower end surface of the screen is 0.5-2cm higher than the lower end surface of the screening frame.
[0037] See also Figure 2 As shown, in order to facilitate the collection of the mixture of soil and clean water, a collecting vessel 11 is provided on the upper end surface of the base 10 and is located directly below the three-stage screening frame. Compared with the prior art, the three-stage screen 9 can be separated from the flowing sewage by a certain distance to prevent the sewage from contaminating the three-stage screen 9.
[0038] See also Figure 2 and Figure 5As shown, the three-level screening frame 3 is hung on the base 10, and a gap is left between the three-level screening frame 3 and the upper end surface of the base 10. Regarding the specific structure of the base 10, the base 10 includes a bottom plate 101 and a support frame arranged on one side of the bottom plate 101, and the outer side of the bottom screening frame is hung on the support frame. The specific structure of the support frame includes a vertical rod 102 and an annular rod 103 fixed as one, the lower end of the vertical rod 102 is connected to the base 10, and the upper end is connected to the horizontally arranged annular rod 103, and the three-level screening frame 3 is hung on the annular rod 103 through a hook 12, and the hook 12 can be slidably connected to the annular rod 103, so that the three-level screening frame 3 can rotate on the annular rod 103 along its circumferential direction, and a plurality of hooks 12 are provided, which are located outside the three-level screening frame 3 and are located in the same horizontal plane.
[0039] When the mixture of soil sample and water flows downward from the primary screen 7, the flow rate is slow, and it may also accumulate on one side to cause blockage, affecting the downward flow of the mixture. For this reason, the three-stage screening frame 3 can be rotated to drive the mixture on the primary screen 7, the secondary screen 8 and the tertiary screen 9 to flow quickly. In conjunction with the above-mentioned operation of moving the screening frame up and down, the separation efficiency is improved, so that there is no liquid residue on the screens of different heights, which is convenient for collecting mycorrhizal fungi.
Claims
1. A multi-stage screening system for highly active targeted microbial strains, characterized in that: It comprises a base (10) placed on a reference surface, a plurality of screening frames are arranged above the base (10) from bottom to top, each screening frame is provided with a corresponding screen, the apertures of the plurality of screens decrease from top to bottom, and the bottom screening frame is hung on the base (10) and is spaced apart from the upper end surface of the base (10); Adjacent screening frames are plug-connected, the upper end surface of a single screening frame is provided with a plug rod, the lower end surface of the screening frame is provided with a slot (6) corresponding to the plug rod, and the screening frame located above the lowest screening frame can rotate around the plug rod; The insertion rods comprise a first insertion rod (4) and a second insertion rod (5) which are vertically arranged on the upper end surface of the screening frame, and the length of the first insertion rod (4) is longer than that of the second insertion rod (5).
2. A multi-stage screening system for highly active targeted microbial strains according to claim 1, characterized in that: The length of the first insertion rod (4) is 1 to 2 times the length of the second insertion rod (5).
3. A multi-stage screening system for highly active targeted microbial strains according to claim 2, characterized in that: The first insertion rod (4) and the second insertion rod (5) are symmetrically arranged on both sides of the screening frame.
4. A multi-stage screening system for highly active targeted microbial strains according to claim 3, characterized in that: A plurality of first insertion rods (4) on the screening frame are coaxially arranged.
5. A multi-stage screening system for highly active targeted microbial strains according to claim 1, characterized in that: The aperture of the screen in the bottom screening frame is 55-100 μm.
6. A multi-stage screening system for highly active targeted microbial strains according to claim 1, characterized in that: The upper end surface of the base (10) is provided with a collecting container (11) located below the screening frame.
7. A multi-stage screening system for highly active targeted microbial strains according to claim 1, characterized in that: The lower end surface of the screen is 0.5-2 cm higher than the lower end surface of the screening frame.
8. A multi-stage screening system for highly active targeted microbial strains according to claim 1, characterized in that: The base (10) comprises a bottom plate (101) and a support frame arranged on one side of the bottom plate (101), and the outer side of the lowest screening frame is hung on the support frame.
9. A multi-stage screening system for highly active targeted microbial strains according to claim 8, characterized in that: The support frame comprises a vertical rod (102) and an annular rod (103) fixedly connected as one body, the lower end of the vertical rod (102) is connected to the base (10), and the upper end is connected to the horizontally arranged annular rod (103), and the lowest screening frame is hung on the annular rod (103) through a hook (12) and can rotate.