A culture dish for rapid detection of aerobic bacteria

By designing aerobic bacteria rapid detection culture dish and using a combination of hollow structure, ventilation holes and air filter membranes, the problems of sterile environment not being closed, bacterial fluid diffusion and anaerobic state in the prior art are solved, and more efficient microbial culture and detection are achieved.

CN222744296UActive Publication Date: 2025-04-11LIAONING AGRI COLLEGE
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Patent Information

Application Number
CN202421027855.9
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-05-11
Publication Date
2025-04-11
Estimated Expiration
2034-05-11

AI Technical Summary

Technical Problem

The existing rapid detection device for total colony counts has the problem of insufficient sterile environment, easy diffusion and spillover of bacterial fluid, and easy formation of an anaerobic state in the culture area, which affects the growth and detection accuracy of microorganisms.

Method used

A quick detection petri dish of aerobic bacteria was designed, using a hollow structure of the Petri dish and multiple ventilation holes and air filters were installed at the bottom. Combined with a sliding cover made of boron glass, it formed a closed and ventilated culture environment to reduce the occurrence of pollution and anaerobic state.

Benefits of technology

It effectively improves the enclosure of the sterile environment, reduces the risk of bacterial fluid diffusion, ensures the aerobic state in the culture area, and improves the growth environment and detection accuracy of microorganisms.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model provides a rapid aerobic bacteria detection culture dish, comprising a culture dish and a sliding cover, wherein the sliding cover cooperates with the culture dish, the culture dish is covered by the sliding cover, the outer diameter of the culture dish matches the inner diameter of the sliding cover, a culture area is arranged at the bottom of the culture dish, a plurality of ventilation holes are arranged in the culture area, the ventilation holes run through the bottom of the culture dish, an air filter membrane is attached to the lower surface of the bottom of the culture dish, and compared with the prior art, the utility model has the following advantages: under the condition of complying with the experimental operation regulations, the operation is convenient and the process is simple; compared with conventional laboratory culture dishes, the bottom of the culture dishes are all provided with air filter membranes, which can effectively filter out interfering substances in the air and are more suitable for the cultivation of aerobic bacteria; the use of conventional culture dishes is changed from flipping to sliding, thereby reducing the possibility of pollution caused by operation problems.
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Description

Technical Field

[0001] The utility model relates to the technical field of agricultural experimental devices, and in particular to a rapid aerobic bacteria detection culture dish. Background Art

[0002] The rapid colony count detection device currently used in China is generally composed of a base, a test piece and a covering film. The disadvantages of this device are mainly reflected in: 1. The sterile environment is not closed enough and is easily polluted by the environment during inoculation and culture; 2. There is no fence on the edge of the paper, and the inoculated bacterial solution is easy to spread and overflow, affecting accuracy; 3. After inoculation of the bacterial solution, the covering film and the rapid test paper are easily absorbed, forming an anaerobic state in the culture area, affecting the growth of microorganisms. Utility Model Content

[0003] In response to the technical problems raised above, a culture dish for rapid detection of aerobic bacteria is provided.

[0004] The technical means adopted by the utility model are as follows:

[0005] A rapid aerobic bacteria detection culture dish comprises a culture dish and a sliding cover. The sliding cover cooperates with the culture dish, the culture dish is covered by the sliding cover, the outer diameter of the culture dish matches the inner diameter of the sliding cover, a culture area is provided at the bottom of the culture dish, a plurality of ventilation holes are provided in the culture area, the ventilation holes penetrate the bottom of the culture dish, and an air filter membrane is attached to the lower surface of the bottom of the culture dish.

[0006] Furthermore, a latch is fixedly connected to the bottom of the sliding cover, and a mounting hole cooperating with the latch is provided on the top of the culture dish.

[0007] Furthermore, the culture dish is a hollow structure, the thickness of the side wall of the culture dish is 0.5 cm to 1.5 cm, the height of the inner wall of the culture dish is 1.5 cm to 2.5 cm, and the diameter of the culture area is 7.5 cm to 8.5 cm.

[0008] Furthermore, the culture dish has a height of 2.5 cm to 3.5 cm and an outer diameter of 9.5 cm to 10.5 cm.

[0009] Furthermore, the diameter of the ventilation hole is 1 cm to 2 cm.

[0010] Furthermore, the height of the latch is 1 cm to 2 cm, and the diameter of the mounting hole is 0.4 cm to 0.6 cm.

[0011] Furthermore, the sliding cover has a thickness of 0.2 cm to 0.4 cm.

[0012] Furthermore, the sliding cover is made of high-boron glass.

[0013] Compared with the prior art, the utility model has the following advantages:

[0014] Under the condition of complying with the experimental operating procedures, the operation is convenient and the process is simple; compared with conventional laboratory culture dishes, the bottom of the culture dishes are equipped with air filters to effectively filter out interfering substances in the air, making them more suitable for the cultivation of aerobic bacteria; the use of conventional culture dishes is changed from flipping to sliding, reducing the possibility of contamination caused by operational problems. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0016] Figure 1 A schematic diagram of the structure of a culture dish for rapid detection of aerobic bacteria provided by the utility model.

[0017] Figure 2 The utility model provides a schematic diagram of the sliding cover structure of a rapid aerobic bacteria detection culture dish.

[0018] In the figure: 1. sliding cover; 2. culture dish; 3. ventilation hole; 4. mounting hole; 5. latch; 6. culture area. DETAILED DESCRIPTION

[0019] It should be noted that, in the absence of conflict, the embodiments and features of the embodiments of the present invention can be combined with each other. The present invention will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0020] In order to make the purpose, technical scheme and advantages of the embodiments of the utility model clearer, the technical scheme in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. The following description of at least one exemplary embodiment is actually only illustrative and is by no means a limitation on the utility model and its application or use. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the utility model.

[0021] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the utility model. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, it indicates the presence of features, steps, operations, devices, components and / or combinations thereof.

[0022] Unless otherwise specifically stated, the relative arrangement, numerical expressions and numerical values ​​of the parts and steps set forth in these embodiments do not limit the scope of the utility model. At the same time, it should be clear that, for ease of description, the sizes of the various parts shown in the accompanying drawings are not drawn according to the actual proportional relationship. The technology, method and equipment known to those of ordinary skill in the relevant field may not be discussed in detail, but in appropriate cases, the technology, method and equipment should be considered as a part of the specification. In all examples shown and discussed here, any specific value should be interpreted as being merely exemplary, rather than as a limitation. Therefore, other examples of exemplary embodiments may have different values. It should be noted that similar reference numerals and letters represent similar items in the following drawings, and therefore, once a certain item is defined in an accompanying drawing, it does not need to be further discussed in subsequent drawings.

[0023] In the description of the present utility model, it needs to be understood that the directions or positional relationships indicated by directional words such as "front, back, up, down, left, right", "lateral, vertical, perpendicular, horizontal" and "top, bottom" are usually based on the directions or positional relationships shown in the drawings. They are only for the convenience of describing the present utility model and simplifying the description. Unless otherwise stated, these directional words do not indicate or imply that the device or element referred to must have a specific direction or be constructed and operated in a specific direction. Therefore, they cannot be understood as limiting the scope of protection of the present utility model: the directional words "inside and outside" refer to the inside and outside relative to the contours of each component itself.

[0024] For ease of description, spatially relative terms such as "above", "above", "on the upper surface of", "above", etc. may be used here to describe the spatial positional relationship between a device or feature and other devices or features as shown in the figure. It should be understood that spatially relative terms are intended to include different orientations of the device in use or operation in addition to the orientation described in the figure. For example, if the device in the accompanying drawings is inverted, the device described as "above other devices or structures" or "above other devices or structures" will be positioned as "below other devices or structures" or "below their position devices or structures". Thus, the exemplary term "above" can include both "above" and "below". The device can also be positioned in other different ways (rotated 90 degrees or in other orientations), and the spatially relative descriptions used here are interpreted accordingly.

[0025] In addition, it should be noted that the use of terms such as "first" and "second" to limit components is only for the convenience of distinguishing the corresponding components. If not otherwise stated, the above terms have no special meaning and therefore cannot be understood as limiting the scope of protection of the utility model.

[0026] like Figures 1-2 As shown, the utility model provides a rapid detection culture dish for aerobic bacteria, including a culture dish 2 and a sliding cover 1. The sliding cover is made of high boron glass to facilitate observation of the reaction state. The sliding cover 1 cooperates with the culture dish 2, and the culture dish 2 is covered by the sliding cover 1. The outer diameter of the culture dish 2 is consistent with the inner diameter of the sliding cover 1. A culture area 6 is provided at the bottom of the culture dish 2, and a plurality of ventilation holes 3 are provided in the culture area 6. The ventilation holes 3 run through the bottom of the culture dish 1, and an air filter membrane is attached to the lower surface of the bottom of the culture dish 2.

[0027] A latch 5 is fixedly connected to the bottom of the sliding cover 1, and a mounting hole 4 matching with the latch 5 is provided on the top of the culture dish 2. The latch 5 is slidably connected to the mounting hole 4. The height of the latch 5 is 1 cm to 2 cm, and the diameter of the mounting hole 4 is 0.4 cm to 0.6 cm.

[0028] The culture dish 2 is a hollow structure, the side wall thickness of the culture dish 2 is 0.5 cm to 1.5 cm, the inner wall height of the culture dish 2 is 1.5 cm to 2.5 cm, and the culture area 6 has a diameter of 7.5 cm to 8.5 cm.

[0029] The culture dish 2 has a height of 2.5 cm to 3.5 cm and an outer diameter of 9.5 cm to 10.5 cm.

[0030] The diameter of the ventilation hole 3 is 1 cm to 2 cm. The thickness of the sliding cover 1 is 0.2 cm to 0.4 cm.

[0031] Working principle of the utility model: Preliminary preparation: soak the non-woven fabric in the prepared culture medium liquid, take it out and dry it after it is completely covered, cut it after sterilization, sterilize it again, seal it in a vacuum bag and store it for later use. Cut the air filter membrane according to the specifications, sterilize it, seal it in a vacuum bag and store it for later use.

[0032] When in use, take out the air filter membrane and attach it to the ventilation hole 3 of the culture dish 2, then place the prepared non-woven fabric in the culture area 6, transfer the diluted diluent with a pipette in the clean bench, open the sliding cover 1, add the diluent, and then seal the culture dish 2 with the sliding cover 1.

[0033] Then put the whole thing into the incubator and set the time and temperature according to the national standard and wait for the cultivation to be completed.

[0034] After being taken out, the culture dish 2 is made of transparent material and can be marked and counted with a pen on the outer shell of the culture dish.

[0035] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the utility model, rather than to limit it. Although the utility model has been described in detail with reference to the aforementioned embodiments, ordinary technicians in this field should understand that they can still modify the technical solutions recorded in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not make the essence of the corresponding technical solution deviate from the scope of the technical solution of the embodiments of the utility model.

Claims

1. A rapid aerobic bacteria detection culture dish, characterized in that: The invention comprises a culture dish and a sliding cover, wherein the sliding cover cooperates with the culture dish, the culture dish is covered by the sliding cover, the outer diameter of the culture dish matches the inner diameter of the sliding cover, a culture area is arranged at the bottom of the culture dish, a plurality of ventilation holes are arranged in the culture area, the ventilation holes penetrate the bottom of the culture dish, and an air filter membrane is attached to the lower surface of the bottom of the culture dish.

2. The rapid aerobic bacteria detection culture dish according to claim 1, characterized in that: A latch is fixedly connected to the bottom of the sliding cover, and a mounting hole matching the latch is provided on the top of the culture dish.

3. A rapid aerobic bacteria detection culture dish according to claim 1 or 2, characterized in that: The culture dish is a hollow structure, the thickness of the side wall of the culture dish is 0.5 cm to 1.5 cm, the height of the inner wall of the culture dish is 1.5 cm to 2.5 cm, and the diameter of the culture area is 7.5 cm to 8.5 cm.

4. A rapid aerobic bacteria detection culture dish according to claim 1 or 2, characterized in that: The culture dish has a height of 2.5 cm to 3.5 cm and an outer diameter of 9.5 cm to 10.5 cm.

5. A rapid aerobic bacteria detection culture dish according to claim 1 or 2, characterized in that: The diameter of the ventilation hole is 1 cm to 2 cm.

6. The rapid aerobic bacteria detection culture dish according to claim 2, characterized in that: The height of the latch is 1 cm to 2 cm, and the diameter of the mounting hole is 0.4 cm to 0.6 cm.

7. A rapid aerobic bacteria detection culture dish according to claim 1 or 2, characterized in that: The thickness of the sliding cover is 0.2 cm to 0.4 cm.

8. A rapid aerobic bacteria detection culture dish according to claim 1 or 2, characterized in that: The sliding cover is made of high-boron glass.