Sugarcane tissue culture equipment

Abstract

The utility model relates to the technical field of tissue culture, in particular to sugarcane tissue culture equipment. Comprising a culture box and an auxiliary device, the auxiliary device comprises a sealing cover, a degerming film, a limiting seat, a temperature sensor, a steel wire mesh pore plate and a stabilizing assembly, degerming holes are formed in the sealing cover, during culture, sugarcane tissue and a culture solution are placed in the culture box, then the sealing cover is installed, and during culture, the temperature sensor is used for monitoring internal temperature; the sterilization film filters bacteria in the air, water vapor generated in the culture box can be discharged from the sterilization holes by means of the semi-permeable performance of the film, meanwhile, the air can be exchanged, healthy development of sugarcane calluses can be guaranteed, the sealing cover can be directly and rotationally detached from the culture box, and the sealing cover is convenient to use. Therefore, when sugarcane callus culture is carried out, ventilation and bacteria filtration can be better achieved, rapid disassembly and assembly are facilitated, and culture and use are facilitated.

Description

Technical Field

[0001] The utility model relates to the technical field of tissue culture, in particular to a sugarcane tissue culture device. Background Art

[0002] Sugarcane callus originally refers to the newly formed tissue on the wound surface after local trauma of sugarcane. It is mostly composed of living parenchyma cells and can be used for subculture and differentiation culture.

[0003] At present, culture dishes are mostly used for sugarcane callus culture. However, since sugarcane callus will produce a large amount of water vapor during the cultivation process, it is easy to have adverse effects on the callus and hinder its differentiation and proliferation. In addition, traditional culture dishes with good sealing have poor ventilation performance, while those with good ventilation performance have poor air filtering and sealing. Traditional culture dishes need to be sealed with sealing film, which increases the work intensity. The sealing film is also easy to break after a long time of use, and even causes pollution, which is not conducive to cultivation and use. Utility Model Content

[0004] The utility model aims to provide a sugarcane tissue culture device, which aims to better realize ventilation and bacteria filtration when culturing sugarcane callus tissue, and is conducive to rapid disassembly and assembly, so as to facilitate cultivation and use.

[0005] To achieve the above-mentioned purpose, the utility model provides a sugarcane tissue culture device, including a culture box and an auxiliary device;

[0006] The auxiliary device includes a sealing cover, a sterilization film, a limiting seat, a temperature sensor, a wire mesh plate and a stabilizing component. The sealing cover is provided with a sterilization hole, the sterilization hole passes through the sealing cover and is located at the top of the sealing cover. The sealing cover is threadedly connected to the culture box and is located at the top of the culture box. The sterilization film is arranged in the sterilization hole. The limiting seat is detachably connected to the sealing cover and is located on the side of the sealing cover close to the sterilization film. The temperature sensor is detachably connected to the culture box and is located at the front side of the culture box. The wire mesh plate is fixedly connected to the limiting seat and is located on the limiting seat. The stabilizing component is arranged on both sides of the bottom of the culture box.

[0007] Wherein, a mounting groove is arranged on the top end surface of the culture box.

[0008] Wherein, the auxiliary device further comprises a sealing gasket, and the sealing gasket is arranged in the mounting groove.

[0009] Wherein, the stabilizing component comprises a hand pump and a pneumatic suction cup, the hand pump is fixedly connected to the culture box and is located at both sides of the bottom of the culture box; the pneumatic suction cup is arranged below the hand pump.

[0010] Wherein, the auxiliary device also includes an addition tube and a threaded cover, wherein the addition tube is threadedly connected to the sealing cover and is located on the sealing cover; the threaded cover is threadedly connected to the addition tube and is located on the top of the addition tube.

[0011] The utility model discloses a sugarcane tissue culture equipment, the top of the culture box is an installation threaded end, which is convenient for direct installation and cooperation of the sealing cover threaded cavity, a sterilization hole is arranged on the sealing cover, a sterilization film is arranged in the sterilization hole and is fixed by a limit seat, a wire mesh plate is arranged on the top of the limit seat, and is used for the safety protection of the sterilization film, a temperature sensor is arranged on the front side of the culture box, and is used for internal temperature monitoring, a stabilizing component is arranged on both sides of the bottom of the culture box, and improves the placement stability of the culture box, during cultivation, the sugarcane tissue and culture solution are placed in the culture box, and then the sealing cover is installed, during cultivation, the temperature sensor is used for internal temperature monitoring, the sterilization film filters bacteria in the air, and utilizes the semi-permeable performance of the membrane to enable the water vapor generated inside the culture box to be discharged from the sterilization hole, and at the same time, the air can also be exchanged, which is beneficial to ensure the healthy development of the sugarcane callus tissue, and the sealing cover can be directly rotated and removed from the culture box, so as to achieve better ventilation and bacteria filtering when the sugarcane callus tissue is cultivated, and is beneficial to quick disassembly and assembly, and is convenient for cultivation and use. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art are briefly introduced below.

[0013] Figure 1 It is a schematic diagram of the overall structure of the sugarcane tissue culture device of the first embodiment of the utility model.

[0014] Figure 2 It is a schematic diagram of the setting position of the installation groove of the first embodiment of the utility model.

[0015] Figure 3 It is a schematic diagram of the installation position of the sterilization membrane of the first embodiment of the utility model.

[0016] Figure 4 It is a schematic diagram of the overall structure of a sugarcane tissue culture device according to the second embodiment of the utility model.

[0017] In the figure: 101-culture box, 102-sealing cover, 103-sterilization film, 104-limiting seat, 105-temperature sensor, 106-wire mesh plate, 107-sterilization hole, 108-installation groove, 109-sealing pad, 110-hand pump, 111-pneumatic suction cup, 201-addition tube, 202-threaded cover. DETAILED DESCRIPTION

[0018] Embodiments of the present invention are described in detail below, and examples of the embodiments are shown in the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present invention, but should not be understood as limiting the present invention.

[0019] Embodiment 1:

[0020] like Figure 1 to Figure 2 As shown, Figure 1 This is the overall structural diagram of the sugarcane tissue culture equipment. Figure 2 is a schematic diagram of the installation position of the installation slot 108, Figure 3 It is a schematic diagram of the installation position of the sterilization film 103. The utility model provides a sugarcane tissue culture device: including a culture box 101 and an auxiliary device, the auxiliary device includes a sealing cover 102, a sterilization film 103, a limit seat 104, a temperature sensor 105, a steel mesh plate 106, a sealing gasket 109 and a stabilizing component. The top end surface of the culture box 101 is provided with a mounting groove 108, the stabilizing component includes a hand pump 110 and a pneumatic suction cup 111, and a sterilization hole 107 is provided on the sealing cover 102. Through the above scheme, it can be achieved that when the sugarcane callus tissue is cultured, ventilation and bacteria filtration can be better achieved, and it is convenient for rapid disassembly and assembly, and convenient for culture and use. It can be understood that the above scheme can be more conducive to culture and use when the sugarcane callus tissue is cultured.

[0021] In this embodiment, the top of the culture box 101 is an externally threaded mounting end, and sugarcane tissue and culture solution can be stored inside.

[0022] Among them, a sterilization hole 107 is opened on the sealing cover 102, and the sterilization hole 107 passes through the sealing cover 102 and is located at the top of the sealing cover 102. The sealing cover 102 is threadedly connected to the culture box 101 and is located at the top of the culture box 101. The sterilization membrane 103 is arranged in the sterilization hole 107. The limiting seat 104 is detachably connected to the sealing cover 102 and is located on the side of the sealing cover 102 close to the sterilization membrane 103. The temperature sensor 105 is detachably connected to the culture box 101 and is located on the front side of the culture box 101. The wire mesh plate 106 is fixedly connected to the limiting seat 104 and is located on the limiting seat 104. The stabilizing component is arranged on both sides of the bottom of the culture box 101. The sealing cover 102 has an internal thread installation cavity, which is convenient for installation and matching with the culture box 101. The top of the sealing cover 102 is provided with the sterilization hole 107 penetrating therethrough. The sterilization hole 107 is stepped. The sterilization membrane 103 can be of 0.22 μm specification. After being placed in the sterilization hole 107, the limiting seat 104 is fixed by bolts. The middle through hole of the limiting seat 104 is convenient for ventilation and the operation of the sterilization membrane 103. The temperature sensor 105 is installed on the culture box 101 by bolts. The front side is used to monitor the internal temperature of the culture box 101. During cultivation, after the culture solution is added, the temperature sensor 105 will not be immersed. The temperature sensor 105 is externally mounted in conjunction with the electrical control box. The wire mesh plate 106 is installed on the top of the limit seat 104 by bolts and has ventilation holes for the safety protection of the sterilization membrane 103 to prevent heavy objects from falling and piercing the sterilization membrane 103. At the same time, ventilation can be performed. The stabilizing component is arranged on both sides of the bottom of the culture box 101 to improve the working stability of the culture box 101.

[0023] Secondly, a mounting groove 108 is provided on the top end surface of the culture box 101. The mounting groove 108 has an O-shaped cross section and is provided on the top end surface of the culture box 101.

[0024] Then, the auxiliary device further comprises a sealing gasket 109, and the sealing gasket 109 is arranged in the installation groove 108. The sealing gasket 109 can be directly arranged in the installation groove 108, and is used for sealing the installation gap between the culture box 101 and the sealing cover 102.

[0025] Finally, the hand pump 110 is fixedly connected to the culture box 101 and is located on both sides of the bottom of the culture box 101; the cylinder suction cup 111 is arranged below the hand pump 110. The hand pump 110 is installed on the culture box 101 by bolts, and the cylinder suction cup 111 is arranged below the hand pump 110. When in use, after the culture box 101 is placed on the culture table, air can be introduced into the cylinder suction cup 111 by controlling the control knob on the top of the hand pump 110, and then the cylinder suction cup 111 is adsorbed on the culture table to achieve fixation, which can prevent the culture box 101 from tipping over due to external impact and improve stability.

[0026] When using the utility model to culture sugarcane callus, ventilation and bacteria filtration can be better achieved, and it is convenient to disassemble and assemble quickly, which is convenient for cultivation and use. During cultivation, the sugarcane tissue and culture solution are placed in the culture box 101, and then the sealing cover 102 is installed. During cultivation, the temperature sensor 105 can be used for internal temperature monitoring. The bacteria in the air can be filtered through the sterilization membrane 103, and the semi-permeable performance of the membrane can be used to enable the water vapor generated inside the culture box 101 to be discharged from the sterilization hole 107, and the air can also be exchanged normally, which is conducive to ensuring the healthy development of the sugarcane callus. The sealing cover 102 is threadedly connected to the culture box 101 and can be directly rotated and removed from the culture box 101 to achieve convenient disassembly and assembly, thereby achieving better ventilation and bacteria filtration during sugarcane callus culture, and is convenient to disassemble and assemble quickly, which is convenient for cultivation and use.

[0027] Embodiment 2:

[0028] like Figure 4 As shown, Figure 4 20 is a schematic diagram of the overall structure of a sugarcane tissue culture device. On the basis of the first embodiment, the utility model provides a sugarcane tissue culture device, wherein the auxiliary device further includes an addition tube 201 and a threaded cover 202 .

[0029] The addition tube 201 is threadedly connected to the sealing cover 102 and is located on the sealing cover 102; the threaded cover 202 is threadedly connected to the addition tube 201 and is located on the top of the addition tube 201. The threaded mounting portion at the bottom of the addition tube 201 can be directly mounted in the threaded mounting hole of the sealing cover 102, and the threaded cover 202 can be mounted on the top of the addition tube 201 for sealing and easy disassembly.

[0030] In this embodiment, when a certain amount of culture solution needs to be added, the threaded cap 202 can be directly unscrewed and then added without removing the sealing cap 102, further providing a nutrient solution addition channel.

[0031] What is disclosed above is only one or more preferred embodiments of the present application, and cannot be used to limit the scope of rights of the present application. Ordinary technicians in this field can understand that all or part of the processes of implementing the above embodiments and equivalent changes made according to the claims of the present application are still within the scope covered by the present application.

Claims

1. A sugarcane tissue culture device, comprising a culture box, characterized in that: Also includes auxiliary devices; The auxiliary device includes a sealing cover, a sterilization film, a limiting seat, a temperature sensor, a wire mesh plate and a stabilizing component. The sealing cover is provided with a sterilization hole, the sterilization hole passes through the sealing cover and is located at the top of the sealing cover. The sealing cover is threadedly connected to the culture box and is located at the top of the culture box. The sterilization film is arranged in the sterilization hole. The limiting seat is detachably connected to the sealing cover and is located on the side of the sealing cover close to the sterilization film. The temperature sensor is detachably connected to the culture box and is located at the front side of the culture box. The wire mesh plate is fixedly connected to the limiting seat and is located on the limiting seat. The stabilizing component is arranged on both sides of the bottom of the culture box.

2. The sugarcane tissue culture device according to claim 1, characterized in that: A mounting groove is arranged on the top end surface of the culture box.

3. The sugarcane tissue culture device according to claim 2, characterized in that: The auxiliary device further comprises a sealing gasket, which is arranged in the mounting groove.

4. The sugarcane tissue culture device according to claim 1, characterized in that: The stabilizing component comprises a hand pump and a pneumatic suction cup. The hand pump is fixedly connected to the culture box and is located at two sides of the bottom of the culture box. The pneumatic suction cup is arranged below the hand pump.

5. The sugarcane tissue culture device according to claim 1, characterized in that: The auxiliary device also includes an addition tube and a threaded cover. The addition tube is threadedly connected to the sealing cover and is located on the sealing cover; the threaded cover is threadedly connected to the addition tube and is located on the top of the addition tube.

Images