An intubation device for isolated frog heart perfusion experiment

Abstract

The utility model discloses an in vitro frog heart perfusion experiment cannula device, comprising an experimental cannula body, a branch tube plug hole is provided on the outer side of the bottom end of the experimental cannula body, a liquid extraction branch tube for conveniently extracting cannula blood is inserted in the branch tube plug hole; a positioning ring is sleeved on the experimental cannula body below the branch tube plug hole, the outer circumferential surface of the positioning ring is provided with an annular positioning groove that is radially recessed outward, and a restraining ring for fixing the frog heart artery incision is clamped outside the annular positioning groove, and the restraining ring includes two symmetrical splicing rings, and the bottom end of the experimental cannula body is a beveled end. The utility model provides a positioning ring on the outer wall of the bottom end of the experimental connecting test tube. When conducting an experiment, it is only necessary to put the frog heart on the outside of the positioning ring to quickly fix the frog heart, reduce the difficulty of operation and shorten the operation time, ensure the activity of the frog heart so that the experimental results will not be biased; at the same time, a liquid extraction branch tube is inserted on one side of the bottom end of the experimental connecting test tube, and blood or cleaning reagents in the frog heart are extracted before the experimental operation.

Description

Technical Field

[0001] The utility model relates to the technical field of frog heart perfusion experimental devices, in particular to an isolated frog heart perfusion experimental cannula device. Background Art

[0002] Regarding the prior art, the patent number is: CN202123073355.8-A new type of frog heart perfusion experimental cannula, which uses a perfusion tube with a beveled head to insert into the frog heart, and an anticoagulant is coated on the outer wall of the beveled head of the perfusion tube to solve the problem of blockage of the beveled head perfusion tube of the cannula caused by coagulation. However, when the frog heart is connected to the perfusion tube, silk thread needs to be used to bind and fix it.

[0003] Using silk thread to fix the frog heart to the bottom of the test tube is very troublesome and time-consuming. The time-consuming operation can easily affect the activity of the frog heart and cause deviations in the experimental data. Therefore, it does not meet the existing needs. In this regard, we propose an in vitro frog heart perfusion experimental device. Summary of the invention

[0004] The purpose of the utility model is to overcome the defects and shortcomings of the prior art and to provide an in vitro frog heart perfusion experiment cannulation device to solve the problem that the frog heart is fixed to the bottom of the test tube using silk thread proposed in the above background technology, which is very troublesome and time-consuming to operate, and the time-consuming operation can easily affect the activity of the frog heart, resulting in deviations in the experimental data.

[0005] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0006] A cannula device for an isolated frog heart perfusion experiment comprises an experimental cannula body, wherein a branch tube plug hole is arranged on the outer side of the bottom end of the experimental cannula body, and a liquid extraction branch tube for conveniently extracting cannulated blood is inserted in the branch tube plug hole; a positioning ring is sleeved on the experimental cannula body below the branch tube plug hole, and an annular positioning groove which is radially recessed outward is arranged on the outer circumference of the positioning ring, and a restraining ring for fixing the incision of the frog heart artery is clamped outside the annular positioning groove, and the restraining ring comprises two symmetrical splicing rings; and the bottom end of the experimental cannula body is a beveled end.

[0007] The liquid extraction branch pipe is an inverted L-shaped structure, and the length of the horizontal section of the L-shape is less than the diameter D of the experimental cannula body and greater than half I / 2D of the diameter of the experimental cannula body.

[0008] An annular groove is arranged on the inner wall of the branch pipe insertion hole, and a sealing ring is clamped on the inner side of the annular groove.

[0009] The thickness of the sealing ring is greater than the gap between the liquid extraction branch pipe and the branch pipe plug-in hole.

[0010] The positioning ring is formed by rubber injection molding, and the inner surface of the positioning ring is coated with adhesive. The positioning ring is attached to the outer side of the bottom end of the experimental cannula body through the adhesive.

[0011] The bottom end of the positioning ring is a tapered structure for easy insertion.

[0012] Both ends of the splicing ring are provided with plug-in circular grooves for docking, and adsorption magnets are plugged into the inner sides of the plug-in circular grooves. The two ends of the two splicing rings are connected by the magnetic force between the adsorption magnets.

[0013] Compared with the prior art, the beneficial effects of the utility model are:

[0014] 1. The utility model provides a positioning ring on the outer wall of the bottom end of the experimental cannula body. When conducting an experiment, it is only necessary to put the frog heart on the outside of the positioning ring, and put the ends of the two splicing rings on the outside of the frog heart so that the splicing ring and the positioning ring clamp the frog heart in the middle, and fit the two ends of the two splicing rings together so that the two splicing rings are adsorbed by adsorption magnets, so that the frog heart can be quickly fixed, the operation difficulty and operation time are reduced, and the activity of the frog heart is guaranteed so that the experimental results will not be biased;

[0015] 2. The utility model has a branch tube connection hole at one side of the bottom end of the experimental cannula body, and a liquid extraction branch tube is inserted into the branch tube connection hole. During the experiment, the liquid inside the frog heart can be extracted through the liquid extraction branch tube, or blood or cleaning reagent can be extracted from the frog heart before the experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0017] Figure 2 It is a partial structural cross-sectional view of the experimental cannula body of the utility model;

[0018] Figure 3 It is a structural schematic diagram of the restraining ring of the utility model.

[0019] In the figure: 1. Experimental cannula body; 2. Liquid extraction branch pipe; 3. Restraining ring; 31. Splicing ring; 32. Adsorption magnet; 33. Insertion circular groove; 4. Branch pipe insertion hole; 5. Sealing ring; 6. Positioning ring; 7. Annular positioning groove; 8. Anti-slip protrusion. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0021] See attached Figure 1-3 ;

[0022] like Figure 1-Figure 3 As shown, an in vitro frog heart perfusion experimental device includes an experimental cannula body 1, a positioning ring 6 is fixed to the outer side of the bottom end of the experimental cannula body 1, a restraining ring 3 is movably installed on the outer side of the positioning ring 6, and an inwardly recessed annular positioning groove 7 is provided on the outer surface of the positioning ring 6, and the bottom end of the positioning ring 6 is a conical structure for easy insertion.

[0023] The restraint ring 3 includes two symmetrical splicing rings 31, both ends of the two splicing rings 31 are provided with plug-in circular grooves 33, the inner side of the plug-in circular grooves 33 is plugged with adsorption magnets 32, the two ends of the two splicing rings 31 are connected by the magnetic force between the adsorption magnets 32 and the adsorption magnets 32, the ends of the two splicing rings 31 are magnetically adsorbed by the adsorption magnets 32, the connection is fast and stable, and the two splicing rings 31 can be easily separated manually, which is convenient for quickly connecting or removing the frog heart and the experimental cannula body 1.

[0024] A branch tube plug-in hole 4 is provided on the outer side of the bottom end of the experimental cannula body 1, and an annular groove is provided on the inner wall of the branch tube plug-in hole 4. A liquid extraction branch tube 2 is inserted in the middle of the branch tube plug-in hole 4. The liquid extraction branch tube 2 is an inverted L-shaped structure. The length of the horizontal section of the L-shape is less than the diameter D of the experimental cannula body and greater than half I / 2D of the diameter of the experimental cannula body. A sealing ring 5 is clamped on the inner side of the annular groove. The thickness of the sealing ring 5 is greater than the gap size between the liquid extraction branch tube 2 and the branch tube plug-in hole 4. The sealing ring 5 is located between the liquid extraction branch tube 2 and the inner wall of the branch tube plug-in hole 4, which can ensure the stability of the liquid extraction branch tube 2 after passing through the middle of the branch tube plug-in hole 4, and can fill the gap between the branch tube plug-in hole 4 and the liquid extraction branch tube 2 to prevent the medicine left in the experimental cannula body 1 from leaking out along the branch tube plug-in hole 4.

[0025] The positioning ring 6 is made of rubber injection molding, and the inner surface of the positioning ring 6 is coated with adhesive. The positioning ring 6 is attached to the outer side of the bottom end of the experimental cannula body 1 through the adhesive. The rubber positioning ring 6 has a certain elasticity, which is convenient for being stretched and put on the outer side of the experimental cannula body 1.

[0026] When the frog heart perfusion experiment is performed using the above perfusion experimental device, two threads are passed through the right aorta, the distal end is ligated, and a slipknot is tied at the proximal end for later use.

[0027] Lift the distal end line, use ophthalmic scissors to cut an oblique incision at the right aorta near the arterial cone, insert the cannula of the present application structure containing a small amount of Ren's solution into the incision, and ensure that the periphery of the frog heart incision covers the positioning ring 6 on the outside of the bottom end of the experimental cannula body 1, then fit the two splicing rings 31 on the outside of the frog heart, and ensure that the splicing ring 31 is clamped in the arc groove recessed inward on the outer surface of the positioning ring 6, and then fit the two ends of the two splicing rings 31 so that the ends of the two splicing rings 31 are inserted into the adsorption magnet 32 ​​for magnetic adsorption. At this time, the two splicing rings 31 will be located on the outside of the positioning ring 6 and will not be separated. The frog heart is clamped and fixed by the two splicing rings 31 and the positioning ring 6, and the cannula is gently lifted, and all the connected tissues at the distal end of the ligature are cut off to remove the heart from the body. After that, the blood in the cannula is repeatedly rinsed with Ren's solution until it is clear liquid, and the liquid level is kept at 1-2cm. The isolated frog heart specimen was successfully prepared and can be used for experiments. The frog heart can be quickly fixed, reducing the difficulty and time of operation.

[0028] Although this specification is described according to implementation modes, not every implementation mode includes only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

[0029] Therefore, the above description is only a preferred embodiment of the present application and is not intended to limit the scope of implementation of the present application; that is, all equivalent changes made according to the scope of the claims of the present application are within the protection scope of the claims of the present application.

Claims

1. An in vitro frog heart perfusion experiment cannula device, comprising an experimental cannula body, characterized in that: The outer side of the bottom end of the experimental cannula body (1) is provided with a branch tube insertion hole (4), and a liquid extraction branch tube (2) for conveniently extracting cannula blood is inserted into the branch tube insertion hole (4); a positioning ring (6) is sleeved on the experimental cannula body (1) below the branch tube insertion hole (4), and the outer circumferential surface of the positioning ring (6) is provided with an annular positioning groove (7) which is radially recessed outward, and a restraining ring (3) for fixing the incision of the frog's cardiac artery is clamped outside the annular positioning groove (7), and the restraining ring (3) includes two symmetrical splicing rings (31). The bottom end of the experimental cannula body (1) is a beveled end.

2. The intubation device for isolated frog heart perfusion experiment according to claim 1, characterized in that: The liquid extraction branch pipe (2) is an inverted L-shaped structure, and the length of the horizontal section of the L-shape is less than the diameter D of the experimental cannula body and greater than half I / 2D of the diameter of the experimental cannula body.

3. The intubation device for isolated frog heart perfusion experiment according to claim 1, characterized in that: An annular groove is provided on the inner wall of the branch pipe insertion hole (4), and a sealing ring (5) is clamped on the inner side of the annular groove.

4. The intubation device for isolated frog heart perfusion experiment according to claim 3, characterized in that: The thickness of the sealing ring (5) is greater than the gap between the liquid extraction branch pipe (2) and the branch pipe insertion hole (4).

5. The intubation device for isolated frog heart perfusion experiment according to claim 1, characterized in that: The positioning ring (6) is made of rubber injection molding, and the inner surface of the positioning ring (6) is coated with adhesive. The positioning ring (6) is attached to the outer side of the bottom end of the experimental cannula body (1) through the adhesive.

6. The intubation device for isolated frog heart perfusion experiment according to claim 1, characterized in that: The bottom end of the positioning ring (6) is a conical structure for easy insertion.

7. The intubation device for isolated frog heart perfusion experiment according to claim 1, characterized in that: Both ends of the splicing ring (31) are provided with plug-in circular grooves (33) for docking, and the inner side of the plug-in circular groove (33) is plugged with an adsorption magnet (32), and the two ends of the two splicing rings (31) are connected by the magnetic force between the adsorption magnets (32) and the adsorption magnets (32).

Images