Cell culture device supporting co-culture of various cells

Abstract

The utility model relates to the technical field of cell culture, in particular to a cell culture device supporting co-culture of various cells, which comprises a box body and a top cover, a plurality of groups of culture holes are arranged in the box body, a first circulation groove is arranged between adjacent culture holes in a penetrating manner, culture dishes are inserted into the culture holes, and the top cover is arranged on the box body. Second circulation grooves are formed in the two sides of the culture dish in a penetrating mode, exchange grooves are formed in the middles of the circulation grooves, and insertion plates are inserted into the exchange grooves. The cell culture device is provided with more partitions, the modules can be replaced and customized, different modules can be combined according to requirements, single-hole independent culture or multi-hole co-culture can be realized, the interaction of more types of cells between cells and between cells and a culture environment can be researched, the action mechanism of drugs can be discussed, and the cell culture device is more convenient to operate.

Description

Technical Field

[0001] The utility model relates to the technical field of cell cultivation, in particular to a cell cultivation device supporting co-cultivation of multiple cells. Background Art

[0002] In vitro cell co-culture is the mixing and co-culturing of two or more cells (which can be from the same tissue or different tissues) so that the morphology and function of one of the cells are stably expressed and maintained for a long time. This technology can simulate the microenvironment generated in the body, making it easier to better observe the interaction between cells and cells, cells and the culture environment, and explore the mechanism of action and possible targets of drugs, filling the gap between monolayer cell culture and whole animal experiments.

[0003] Existing co-culture technology solutions mainly rely on cell chambers, but the chambers are inconvenient to use and operate, expensive, and most importantly, they are not applicable to co-culture of more types of cells (more than two). Utility Model Content

[0004] The utility model provides a cell culture device supporting co-culture of multiple cells in order to solve the problems of the prior art.

[0005] The purpose of the utility model can be achieved through the following technical solutions: A cell culture device supporting co-culture of multiple cells, comprising: a box body and a top cover, wherein a plurality of groups of culture wells are arranged inside the box body, a first flow slot is arranged through between adjacent culture wells, a culture dish is inserted into the culture well, second flow slots are arranged through on both sides of the culture dish, a first exchange slot is arranged in the middle of the first flow slot, and an insert is inserted into the first exchange slot.

[0006] As a further improvement, the plug board is arranged in a closed manner.

[0007] As a further improvement, a second exchange slot is provided through the middle of the plug plate, and a material exchange membrane is provided in the second exchange slot.

[0008] As a further improvement, a guide block is disposed outside the second flow channel of the culture dish, and the guide block can be slidably disposed in the first flow channel.

[0009] As a further improvement, an avoidance groove is arranged on the outer side of the top end of the box body, and the bottom end of the top cover is sleeved on the outer side of the avoidance groove.

[0010] As a further improvement, a sealing ring is provided inside the top cover, and the sealing ring and the culture hole are provided correspondingly.

[0011] Compared with the prior art, the utility model has the following beneficial effects: the utility model has more partitions, the modules can be replaced and customized, and different modules can be combined according to needs to realize single-well separate culture, or 2, 3, or 4-well co-culture, which can study the interaction between more types of cells and between cells and culture environment, and explore the mechanism of action of drugs, and the operation is more convenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 It is a schematic diagram of the structure of the utility model;

[0013] Figure 2 It is a partial structural schematic diagram of the utility model;

[0014] Figure 3 It is a partial cross-sectional view of the utility model;

[0015] Figure 4 It is a structural schematic diagram of the top cover of the utility model.

[0016] In the figure, 1, box body; 11, culture hole; 111, first circulation slot; 112, first exchange slot; 12, avoidance slot; 2, top cover; 21, sealing ring; 3, culture dish; 31, second circulation slot; 311, guide block; 4, plug plate; 41, second exchange slot. DETAILED DESCRIPTION

[0017] In the description of the present invention, it should be noted that the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside", etc. indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, they cannot be understood as limitations on the present invention.

[0018] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0019] Below is a combination of the embodiments and the attached Figures 1 to 4 , the technical solution of the utility model is further elaborated.

[0020] Example 1

[0021] A cell culture device supporting co-culture of multiple cells comprises: a box body 1 and a top cover 2, wherein a plurality of culture wells 11 are arranged inside the box body 1, a first flow slot 111 is provided between adjacent culture wells 11, a culture dish 3 is inserted into the culture well 11, second flow slots 31 are provided on both sides of the culture dish 3, a first exchange slot 112 is provided in the middle of the first flow slot 111, an insert plate 4 is inserted into the first exchange slot 112, the insert plate 4 can be selected as a closed insert plate or an insert plate with a second exchange slot 41 provided in the middle, and a substance exchange membrane is provided in the second exchange slot 41.

[0022] like Figures 1 to 4 As shown, the utility model has a simple structure. In actual use, first, according to actual experimental needs, the culture dish 3 is placed in the culture hole 11, and a closed plug plate or a plug plate with a second exchange slot 41 running through the middle is selected to be inserted into the first exchange slot 112 to achieve isolation between the culture dishes 3. The second exchange slot 41 of the plug plate 4 is provided with a material exchange membrane that can be used for cell migration, transport and other functions. After assembly is completed, different cells are connected to different culture dishes 3 to achieve co-culture of cells; when so many types of cells are not needed for co-culture, it is only necessary to insert the closed plug plate 4 into the first exchange slot 112 to seal the second flow slot 31 of the culture dish 3. At the same time, the culture dish 3 without the second flow slot 31 can also be used to achieve single-hole independent culture.

[0023] Compared with the current cell chamber with only two separated areas, the utility model has more partitions, and the modules can be replaced and customized. Different modules can be combined according to needs to achieve single-well culture, or 2, 3, or 4-well co-culture. It can study the interactions between more types of cells, between cells and culture environments, and explore the mechanism of action of drugs, and the operation is more convenient.

[0024] The material of the material exchange membrane is polycarbonate, and the pore size is optional. Membranes with different pore sizes can be selected according to different experiments.

[0025] In a further preferred embodiment, a guide block 311 is provided on the outer side of the second flow groove 31 of the culture dish 3, and the guide block 311 can be slidably disposed in the first flow groove 111. The culture dish 3 is limited by the guide block 311, thereby improving the stability of the culture dish 3 during the installation process.

[0026] As a further preferred embodiment, an escape groove 12 is provided on the outer side of the top end of the box body 1, and the bottom end of the top cover 2 is sleeved on the outer side of the escape groove 12 to facilitate the assembly of the equipment.

[0027] As a further preferred embodiment, a sealing ring 21 is provided inside the top cover 2, and the sealing ring 21 and the culture hole 11 are provided correspondingly to further improve the stability of the device.

[0028] The preferred specific embodiments of the utility model are described in detail above. It should be understood that ordinary technicians in this field can make many modifications and changes based on the concept of the utility model without creative work. Therefore, all technical solutions that can be obtained by technicians in this technical field based on the concept of the utility model through logical analysis, reasoning or limited experiments on the basis of the existing technology should be within the scope of protection determined by the claims.

Claims

1. A cell culture device supporting co-culture of multiple cells, characterized in that: include: A box body (1) and a top cover (2), wherein a plurality of culture wells (11) are arranged inside the box body (1), a first circulation groove (111) is arranged between adjacent culture wells (11), a culture dish (3) is inserted into the culture well (11), second circulation grooves (31) are arranged on both sides of the culture dish (3), a first exchange groove (112) is arranged in the middle of the first circulation groove (111), and an insert plate (4) is inserted into the first exchange groove (112).

2. A cell culture device supporting co-culture of multiple cells according to claim 1, characterized in that: The plug board (4) is arranged in a closed manner.

3. A cell culture device supporting co-culture of multiple cells according to claim 1, characterized in that: A second exchange slot (41) is provided through the middle of the plug plate (4), and a material exchange membrane is provided in the second exchange slot (41).

4. A cell culture device supporting co-culture of multiple cells according to claim 1, characterized in that: A guide block (311) is arranged outside the second circulation groove (31) of the culture dish (3), and the guide block (311) can be slidably arranged in the first circulation groove (111).

5. The cell culture device supporting co-culture of multiple cells according to claim 1, characterized in that: The outer side of the top end of the box body (1) is provided with an avoidance groove (12), and the bottom end of the top cover (2) is sleeved on the outer side of the avoidance groove (12).

6. The cell culture device supporting co-culture of multiple cells according to claim 1, characterized in that: A sealing ring (21) is arranged inside the top cover (2), and the sealing ring (21) and the culture hole (11) are arranged correspondingly.

Images