Row-up electrochemical reagent reaction strip suitable for simultaneous operation of porous pipettes

Abstract

The utility model discloses a row electrochemical reagent reaction strip suitable for simultaneous operation of a porous pipette, the row electrochemical reagent reaction strip consists of eight independent electrochemical test strips which are transversely connected, and each independent electrochemical test strip is sequentially provided with a PET (Polyethylene Terephthalate) substrate layer, a conductive layer, an insulating oil layer, a double-sided adhesive layer and a cover plate layer from bottom to top, the conductive layer is provided with a detection electrode group, the insulating oil layer covers the part above the pins of the detection electrode group, a reaction area is arranged at one end far away from the corners of the detection electrode group, and a reagent reaction chamber is arranged on the cover plate layer. According to the multi-hole pipette, the row design is adopted, the distance between the reaction chambers is kept consistent with the distance between the pipette heads of the row pipette, the use of the multi-hole pipette is facilitated, the consistency of the adding time of the reaction liquid can be ensured, and the consistency of the reaction time can also be ensured.

Description

Technical Field

[0001] The utility model belongs to the technical field of biochemistry, and in particular relates to a linked electrochemical reagent reaction strip suitable for simultaneous operation of multi-hole liquid transfer guns. Background Art

[0002] Electrochemical biosensor technology is a sensor that uses biological materials as sensitive elements and electrodes as conversion elements, and uses potential or current as characteristic detection signals. When using electrochemical biosensors for substance detection, the substance to be tested enters the bioactive material through diffusion, undergoes molecular recognition, and undergoes biochemical reactions. The electron transfer in the process is converted by the electrode into an electrochemical signal that can be qualitatively or quantitatively measured, thereby calculating the concentration of the substance to be tested in the system.

[0003] Electrochemical biosensors can be divided into enzyme sensors, immunosensors, cell sensors, tissue sensors, nucleic acid sensors, microbial sensors, DNA sensors and molecular imprinting sensors based on the recognition element, i.e. the biological material used. According to the different detection signals, they can be divided into amperometric biosensors, voltammetric biosensors, potentiometric biosensors, organic electrochemical transistor biosensors, photoelectrochemical biosensors and electrochemiluminescence biosensors. Among them, amperometric biosensors are the most mature and commercialized electrochemical biosensors. A series of electrochemical test strips for testing blood sugar, uric acid, total cholesterol, blood ketones, etc. on the market belong to this type. In addition, electrochemiluminescence technology is also increasingly valued by researchers in the field of in vitro diagnostic reagents.

[0004] Electrochemical detection reagents are reaction reagents used in conjunction with electrochemical biosensors, including buffers, reaction substrates, biological enzymes, electron mediators and other auxiliary materials. In the current use scenarios of electrochemical biosensors, except for the dry chemical test strips with a high degree of commercialization, the substance to be tested and the reaction reagent are basically mixed and reacted in a certain container, and then connected to the working electrode and the counter electrode to detect the electrochemical signal. This method is simple to operate and does not require additional instruments and equipment. The reaction container and the test electrode can also be changed according to the specific situation to meet different test needs. Therefore, it is widely adopted. As a result, some electrochemical detection kit products with sealed reaction reagents have been developed, but in actual use, there are still problems of cumbersome operation and low test efficiency. At the same time, for the updated electrochemical workstation that has already launched multi-channel testing, there is still no matching electrochemical detection device on the market that can detect multiple samples at the same time, although some manufacturers have launched multi-interface converters to meet the needs of simultaneous detection of multiple samples. However, in terms of design, it is still necessary to insert disposable test strips one by one into the reaction interface, and then use a pipette to transfer the reaction liquid to the electrode reaction area for testing. The measurement process is cumbersome and has certain operating requirements, and the improvement of measurement efficiency is limited. Utility Model Content

[0005] In order to overcome the technical problems in the prior art, the utility model provides a row of electrochemical reagent reaction strips suitable for simultaneous operation of multi-porous pipette guns. The row design is used, and the spacing between reaction chambers is consistent with the spacing between the gun heads of the row of pipette guns, which is convenient for the use of the multi-porous pipette guns, can ensure the consistency of the addition time of the reaction liquid, and can also better ensure the consistency of the reaction time.

[0006] In order to solve the above technical problems, the technical solutions proposed by the utility model are as follows:

[0007] The present application provides a row of electrochemical reagent reaction strips suitable for simultaneous operation of a multi-hole pipette gun, wherein the row of electrochemical reagent reaction strips is composed of 8 independent electrochemical test strips connected laterally, and the independent electrochemical test strips are sequentially provided with a PET substrate layer, a conductive layer, an insulating oil layer, a double-sided adhesive layer and a cover layer from bottom to top.

[0008] The conductive layer is provided with a detection electrode group; the insulating oil layer covers the part above the pins of the detection electrode group, a reaction zone is provided at the end away from the corner of the detection electrode group, the insulating oil layer and the double-sided adhesive layer above the reaction zone are exposed areas, and a boundary is provided between the exposed areas of two adjacent electrochemical test strips; a reagent reaction chamber is provided on the cover layer, and the reagent reaction chamber is provided directly above the reaction zone.

[0009] In the present application, the PET substrate layer is used to support the test strip. The double-sided adhesive layer overlaps with the insulating oil layer structure, and because of the boundary, the reaction areas of adjacent electrochemical test strips are isolated from each other to ensure that the reaction liquids in different reaction areas will not interfere with each other. At the same time, the connected reagent reaction chambers are isolated from each other and will not interfere with each other.

[0010] As an optional embodiment, in the row of electrochemical reagent reaction strips provided in the present application, the reaction zone is arranged to be transversely through-connected, and the width of the reaction zone is 5.0-8.0 mm.

[0011] As an optional implementation, in the row of electrochemical reagent reaction strips provided in the present application, the thickness of the double-sided adhesive layer is 3.0-6.0 mm.

[0012] As an optional embodiment, in the coupled electrochemical reagent reaction strips provided in the present application, the reagent reaction chamber is circular or square and penetrates from top to bottom, and the reagent reaction chamber completely exposes the reaction area.

[0013] As an optional embodiment, in the row of electrochemical reagent reaction strips provided in the present application, the distance between two adjacent reagent reaction chambers is 8.0-9.0 mm, and the height of the reagent reaction chamber is 8.0-10.0 mm.

[0014] As an optional implementation, in each independent electrochemical test strip in the coupled electrochemical reagent reaction strip provided in the present application, the detection electrode group is composed of a working electrode and a counter electrode.

[0015] As an optional embodiment, in the coupled electrochemical reagent reaction strip provided in the present application, the upper end of the detection motor group is a carbon electrode, and the lower end is a silver electrode. The spacing between the carbon electrodes is 0.5-1.2 mm, and the width is 1.5-3.0 mm.

[0016] Based on the same technical concept, the utility model also provides a circuit adapter board, including a PCB board, a workstation electrode line connector and an adapter interface arranged on the PCB board, and the workstation electrode line connector and the adapter interface are connected by an electrical conductor.

[0017] As an optional implementation, in the circuit adapter board provided in the present application, the number of the workstation electrode line connectors is several, and they are arranged at one end of the PCB board and spaced 1.0-1.5 cm apart from each other.

[0018] As an optional implementation, in the circuit adapter board provided in the present application, a copper spring interface is provided on the adapter interface, the number of the spring interfaces is consistent with the number of the workstation electrode line connectors, the spring interfaces are arranged in sequence, and the spacing between two adjacent spring interfaces is 0.5-1.2mm.

[0019] As an optional implementation, in the circuit adapter board provided in the present application, the PCB board has a length of 16-25 cm, a width of 8-16 cm, and a thickness of 0.5-1.0 cm.

[0020] Compared with the prior art, the beneficial effects of the utility model are:

[0021] (1) In the present application, the reaction area of ​​the test electrode is directly assembled at the bottom of the reagent reaction chamber, integrating the reaction step and the test step, simplifying the operation process, reducing the waste of reagents and samples, and being more conducive to the detection of trace samples.

[0022] (2) Using a row design, the spacing between the reaction chambers is consistent with the spacing between the pipette tips, which facilitates the use of multi-hole pipettes, ensures that the reaction solution is added at the same time, and can also ensure the consistency of the reaction time.

[0023] (3) By using a specially designed multi-interface adapter circuit board, it can be connected to a multi-channel electrochemical workstation to ensure that it can measure multiple different samples simultaneously. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0025] Figure 1 This is a schematic diagram of the structure of the coupled electrochemical reagent reaction strips in this application;

[0026] Figure 2 for Figure 1 A top view of

[0027] Figure 3 is a schematic diagram of the structure of an independent electrochemical test strip;

[0028] Figure 4 It is a schematic diagram of the structure of the circuit adapter board.

[0029] Reference numerals:

[0030] 1. electrochemical test strip; 11. PET substrate layer; 12. conductive layer; 13. insulating oil layer; 14. double-sided adhesive layer; 15. cover layer; 121. working electrode; 122. counter electrode; 123. reaction area; 151. reagent reaction chamber;

[0031] 21. PCB board; 22. Workstation electrode line connector; 23. Adapter interface; 24. Electrical conductor; 25. Shrapnel interface. DETAILED DESCRIPTION

[0032] In order to facilitate the understanding of the present invention, the present invention will be described more comprehensively and meticulously below in combination with the specification and preferred embodiments, but the protection scope of the present invention is not limited to the following specific embodiments.

[0033] Unless otherwise defined, all professional terms used below have the same meaning as those generally understood by those skilled in the art. The professional terms used herein are only for the purpose of describing specific embodiments and are not intended to limit the scope of protection of the present utility model.

[0034] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods.

[0035] Example 1

[0036] A parallel electrochemical reagent reaction strip suitable for simultaneous operation of a multi-hole pipette gun, the structural schematic diagram is as follows Figure 1-3 As shown, the row of electrochemical reagent reaction strips consists of 8 independent electrochemical test strips 1 connected horizontally, and each independent electrochemical test strip 1 is provided with a PET substrate layer 11, a conductive layer 12, an insulating oil layer 13, a double-sided adhesive layer 14 and a cover layer 15 in sequence from bottom to top.

[0037] The PET substrate layer 11 is disposed at the bottom to support the test strip.

[0038] The conductive layer 12 is provided with a detection electrode group, and in each independent electrochemical test strip, the detection electrode group is composed of a working electrode 121 and a counter electrode 122. The upper end of the detection electrode group is a carbon electrode, and the lower end is a silver electrode. The spacing between the carbon electrodes is 0.5-1.2 mm, and the width is 1.5-3.0 mm.

[0039] The insulating oil layer 13 covers the part above the pins of the detection electrode group. According to the arrangement of the electrodes, a reaction area 123 is provided at one end away from the pins of the detection electrode group. The insulating oil layer 13 and the double-sided adhesive layer 14 above the reaction area 123 are exposed areas, and a boundary is provided between the exposed areas of two adjacent electrochemical test strips 1. The reaction area 123 is arranged to be through-through in the horizontal direction, with a width of 5.0-8.0 mm, and the other areas in the insulating oil layer 13 cover the working electrode 121 and the counter electrode 122 in the conductive layer.

[0040] The double-sided adhesive layer 14 is used to fix the insulating oil layer 13 and the cover layer 15. Above the reaction area 123, the double-sided adhesive layer 14 is exposed to expose the reaction area. In the present application, a boundary is provided in the longitudinal direction of the exposed area. When the double-sided adhesive layer 14 and the insulating oil layer 13 are structurally overlapped, the reaction areas 123 of different electrode test strips are isolated from each other because of the boundary, ensuring that the reaction liquids in different reaction areas 123 do not interfere with each other. In the present application, the thickness of the double-sided adhesive layer 14 is 3.0-6.0 mm.

[0041] A cover layer 15 is disposed above the double-sided adhesive layer 14 . A reagent reaction chamber 151 is disposed on the cover layer 15 . The reagent reaction chamber 151 is disposed right above the reaction area 123 .

[0042] In the present application, the cover plate with the reagent reaction chamber 151 is made of antistatic polystyrene (PVC). The reagent reaction chamber is circular or square, and the lower end is aligned with the reaction area 123, that is, the reagent reaction chamber 151 completely exposes the reaction area 123. Two adjacent reagent reaction chambers 151 are isolated from each other and will not interfere with each other.

[0043] In this embodiment, the distance between two adjacent reagent reaction chambers 151 is 8.0-9.0 mm, which is consistent with the spacing of the multi-hole row pipette gun tips. The height of the reagent reaction chamber 151 is 8.0-10.0 mm, and can accommodate up to 350 μl of reaction solution.

[0044] Example 2

[0045] In order to connect the electrochemical reagent strips and the multi-channel electrochemical workstation, this embodiment also provides a circuit adapter board, the structural diagram of which is shown in FIG. Figure 4 As shown, it includes a PCB board 21, a workstation electrode line connector 22 and a transfer interface 23 arranged on the PCB board 21, and the workstation electrode line connector 22 and the transfer interface 23 are connected through an electrical conductor 24.

[0046] In the present application, the PCB board 21 plays a supporting role, and has a length of 16-25 cm, a width of 8-16 cm, and a thickness of 0.5-1.0 cm.

[0047] The number of workstation electrode line connectors 22 is a plurality. In this embodiment, the workstation electrode line connectors 22 are made of copper material, the number is 16, and are arranged at one end of the PCB board 21, with a spacing of 1.0-1.5 cm between each other, and are used to connect to the electrode line of the electrochemical workstation.

[0048] A copper spring interface 25 is provided on the adapter interface 23, wherein the number of the spring interfaces 25 is consistent with the number of the workstation electrode line connectors 22, and the spring interfaces 25 are arranged in sequence, one workstation electrode line connector 22 is connected to one spring interface 25 through a wire, and the spacing between two adjacent spring interfaces 25 is 0.5-1.2mm. In the present embodiment, the number of springs is 16, and the springs are arranged in sequence. The width of the springs is 1.5-3.0mm, and the spacing between two adjacent springs is 0.5-1.2mm. The springs are consistent with the electrode arrangement of the reagent reaction strip, and the springs are designed to ensure good conductivity while better fixing the electrochemical reagent reaction strip.

[0049] Example 3

[0050] The steps for simultaneously detecting the concentration of the same indicator to be tested in multiple samples using the coupled electrochemical reagent reaction strips and the circuit adapter board are as follows:

[0051] (1) Neatly insert the detection electrode group of the electrochemical reagent reaction strip into the adapter interface of the circuit adapter board, so that the electrode group on the electrochemical test strip is accurately inserted into the spring interface.

[0052] (2) Fix the detection line with a metal clip on the multi-channel electrochemical workstation to the cylindrical workstation electrode line connector on the circuit adapter board. The signal input line in a detection channel is connected to two adjacent cylindrical connectors, where the working electrode signal input line corresponds to the cylindrical connector of the working electrode of the electrochemical test strip, and the counter electrode signal input line corresponds to the cylindrical connector of the counter electrode of the electrochemical test strip. There are a total of 8 groups of signal input lines, corresponding to eight electrochemical workstation detection channels.

[0053] (3) According to the instructions for use of the reagents for different indicators, add the same volume of blank reagent, standard and liquid sample into the eight reaction chambers of the strip electrochemical reagent reaction strip, and then use a strip pipette to add the same volume of buffer solution and the same volume of electron mediator solution, so that the final solution reaction system completely covers the bottom of the reaction chamber without overflowing, and ensure that there are no bubbles or other obvious solid particles attached to the electrode reaction area at the bottom.

[0054] (4) The detection method is selected on the electrochemical workstation, and then the reaction systems in the eight reaction chambers are measured simultaneously. Finally, the index concentrations of the samples to be tested in different reaction chambers are calculated according to the corresponding calculation formula to complete the detection.

[0055] During the detection process, the volume of blank reagent, standard substance, sample amount, buffer solution, electron mediator, and the corresponding electrochemical detection method and calculation formula are determined according to the technical specifications of electrochemical reagents with different detection indicators.

[0056] The above contents are further detailed descriptions of the present invention in combination with specific preferred implementations, and it cannot be determined that the specific implementation of the present invention is limited to these descriptions. For ordinary technicians in the field to which the present invention belongs, several simple deductions or substitutions can be made without departing from the concept of the present invention, which should be regarded as falling within the scope of protection of the present invention.

Claims

1. A parallel electrochemical reagent reaction strip suitable for simultaneous operation of a multi-hole pipette gun, characterized in that: The row of electrochemical reagent reaction strips is composed of eight independent electrochemical test strips (1) connected transversely, wherein the independent electrochemical test strips (1) are provided with a PET substrate layer (11), a conductive layer (12), an insulating oil layer (13), a double-sided adhesive layer (14) and a cover layer (15) in order from bottom to top; The conductive layer (12) is provided with a detection electrode group; the insulating oil layer (13) covers the portion above the detection electrode group pins, and a reaction area (123) is provided at one end away from the detection electrode group pins; the insulating oil layer (13) and the double-sided adhesive layer (14) above the reaction area (123) are exposed areas, and a boundary is provided between the exposed areas of two adjacent electrochemical test strips; a reagent reaction chamber (151) is provided on the cover layer (15), and the reagent reaction chamber (151) is provided directly above the reaction area (123).

2. The electrochemical reagent strip suitable for simultaneous operation of a multi-hole pipette according to claim 1, characterized in that: The reaction zone (123) is arranged to be transversely through-connected, and the width of the reaction zone is 5.0-8.0 mm.

3. The electrochemical reagent strip suitable for simultaneous operation of a multi-hole pipette according to claim 1, characterized in that: The thickness of the double-sided adhesive layer (14) is 3.0-6.0 mm.

4. The electrochemical reagent strip suitable for simultaneous operation of a multi-hole pipette according to claim 1, characterized in that: The reagent reaction chamber (151) is circular or square and penetrates from top to bottom, and the reagent reaction chamber (151) completely exposes the reaction area (123).

5. The electrochemical reagent strip suitable for simultaneous operation of a multi-hole pipette gun according to any one of claims 1 to 4, characterized in that: The distance between two adjacent reagent reaction chambers (151) is 8.0-9.0 mm, and the height of the reagent reaction chamber (151) is 8.0-10.0 mm.

6. The electrochemical reagent strip suitable for simultaneous operation of a multi-hole pipette according to claim 1, characterized in that: In each independent electrochemical test strip (1), the detection electrode group consists of a working electrode and a counter electrode.

7. The electrochemical reagent strip suitable for simultaneous operation of a multi-hole pipette according to claim 1, characterized in that: The upper end of the detection electrode group is a carbon electrode, and the lower end is a silver electrode. The spacing between the carbon electrodes is 0.5-1.2 mm, and the width is 1.5-3.0 mm.

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