Full-automatic western blot processing system

Abstract

The utility model discloses a full-automatic western blot processing system which comprises a shell with a containing cavity inside. The panel is arranged at the front end of the shell in an openable and closable manner; the kit assembly is arranged at the bottom in the accommodating cavity; the incubation box assembly is arranged on the right side of the kit assembly, and at least two antibody incubation grooves are distributed in the incubation box assembly; the swinging table is arranged at the bottom of the accommodating cavity and is used for driving the incubation box assembly to obliquely rotate and swing front and back; the waste liquid box is arranged on one side of the kit assembly or the incubation box assembly; the liquid transferring assembly comprises a sample adding pipe and a driving device for driving the sample adding pipe to move in the longitudinal direction and the horizontal direction; the cleaning liquid box is arranged on the left side of the waste liquid box; the lower end of the liquid suction pipe is inserted into the cleaning liquid box, the upper end of the liquid suction pipe is communicated with the upper end of the sample adding pipe through a plunger pump, and electromagnetic valves are arranged between the plunger pump and the liquid suction pipe and between the plunger pump and the sample adding pipe. According to the utility model, the functions of automatic sample adding, automatic recovery, automatic cleaning, automatic incubation and the like are realized, and the automation degree is high.

Description

Technical Field

[0001] The utility model belongs to the technical field of incubators, and in particular relates to a full-automatic protein blotting processing system. Background Art

[0002] Western Blot experiment, also known as protein blotting experiment, is a commonly used experimental method in molecular biology, biochemistry and immunogenetics. Its basic principle is to stain cells or biological tissue samples treated by gel electrophoresis with specific antibodies, and to obtain information on the expression of specific proteins in the analyzed cells or tissues by analyzing the position and depth of staining. It is now widely used in gene expression research at the protein level, antibody activity detection and early diagnosis of diseases. This method can be divided into three stages: electrophoresis, transfer, and enzyme immunoassay. The protein sample separated by PAGE (polyacrylamide gel electrophoresis) is transferred to a solid phase carrier (such as nitrocellulose membrane). The solid phase carrier adsorbs proteins in the form of non-covalent bonds and can keep the type of polypeptide separated by electrophoresis and its biological activity unchanged. The protein or polypeptide on the solid phase carrier is used as an antigen to react with the corresponding antibody, and then reacts with the enzyme or isotope-labeled second antibody. The protein component expressed by the specific target gene separated by electrophoresis is detected through substrate color development or radioautography.

[0003] Currently, in Western Blot experiments, after the membrane transfer is completed, the blocking, primary antibody incubation, primary antibody recovery, primary antibody washing, secondary antibody incubation, secondary antibody recovery, and secondary antibody washing cannot be fully automatically controlled. The sample liquid needs to be added manually. After the experiment, the experimental reagents need to be manually poured out and the washing needs to be done manually. The entire process is very cumbersome and inconvenient, with a low degree of automation. In addition, due to incomplete manual operation, there is residual liquid or uneven sample addition, resulting in inaccurate experimental data. Utility Model Content

[0004] The utility model aims to overcome the existing technical defects and provide a fully automatic protein blotting processing system, which can automatically realize the processes of automatic sample addition, automatic incubation, automatic recovery, automatic cleaning and the like after protein transfer and before substrate incubation, with a high degree of automation, and ensure the stability and accuracy of test data.

[0005] In order to solve the above technical problems, the utility model provides a fully automatic protein blotting processing system, comprising:

[0006] A shell having a cavity inside and an opening at the front side of the shell;

[0007] A panel, which is closable and arranged at the front end of the housing and is used to cover or open the opening;

[0008] A reagent kit assembly, which is arranged at the bottom of the chamber and is used to separate and accommodate a plurality of different reagents;

[0009] An incubation box assembly, which is arranged on the right side of the test kit assembly, and at least two antibody incubation tanks are arranged on the incubation box assembly;

[0010] A swing platform, which is arranged at the bottom of the cavity and is used to drive the incubation box assembly to tilt, rotate and swing forward and backward;

[0011] A waste liquid box, which is arranged on the left side of the reagent kit assembly or the right side of the incubation box assembly and is used to collect waste liquid;

[0012] A pipetting assembly, the pipetting assembly comprising a sample loading tube disposed at the upper end of the chamber and used to absorb the reagent in the reagent kit assembly, and a driving device used to drive the sample loading tube to move in the longitudinal and horizontal directions, so that the sample loading tube can move back and forth between the waste liquid box, the reagent kit assembly and the incubation box assembly;

[0013] A cleaning liquid box, which is arranged on the left side of the waste liquid box and is used to contain the cleaning liquid;

[0014] A pipette, the lower end of which is inserted into the cleaning liquid box, the upper end of which is communicated with the upper end of the sample adding tube through a plunger pump, and electromagnetic valves are arranged between the plunger pump and the pipette and the sample adding tube.

[0015] Furthermore, the fully automatic protein blotting processing system also includes a vertical moving device for driving the panel to slide up and down and open, the vertical moving device includes two transmission belts arranged vertically on both sides of the front end of the shell, a transmission shaft connected to the two transmission belts, and a motor for driving the transmission shaft. The front end of the shell is also provided with two guide shafts respectively located in front of the two transmission belts, and a slider is slidably provided on the guide shaft, one side of the slider is fixedly connected to the transmission belt, and the inner upper end or lower end of the panel is fixedly connected to the sliders on the two guide shafts; first synchronous pulleys are provided at both ends of the transmission shaft, and two second synchronous pulleys are rotatably provided in the shell and are respectively located below the first synchronous pulley, and the transmission belt is wound around the first synchronous pulley and the second synchronous pulley; a driven gear is also provided on the transmission shaft, and a driving gear meshing with the driven gear is provided on the output shaft of the motor.

[0016] Furthermore, the reagent kit assembly includes a reagent kit base, a reagent kit upper cover rotatably arranged on the upper end of the reagent kit base, and at least three reagent kits arranged between the reagent kit base and the reagent kit upper cover and used to respectively accommodate different reagents, the reagent kit upper cover is provided with a plurality of first through holes for sample tubes to pass through, a first cooling box and a first temperature conduction plate arranged on the upper end of the first cooling box are provided inside the reagent kit base, the reagent kit is arranged on the first temperature conduction plate, a water inlet and a water outlet are provided on the first cooling box, thermal insulation cotton is provided between the first cooling box and the reagent kit base, and a temperature sensor is provided on the first temperature conduction plate; the reagent kit upper cover and the front end of the reagent kit are both provided with handles.

[0017] Furthermore, the incubation box assembly includes an incubation box base, an incubation box upper cover which is rotatably arranged on the upper end of the incubation box base, and at least two incubation boxes which are arranged left and right between the incubation box base and the incubation box upper cover, the incubation box upper cover is provided with a plurality of second through holes for the sample tubes to pass through, and the upper end of the incubation box is recessed with at least one antibody incubation groove which is arranged left and right; a second cooling box and a second temperature conduction plate which is arranged on the upper end of the second cooling box are provided inside the incubation box base, the incubation box is provided on the second temperature conduction plate, a water inlet and a water outlet are provided on the second cooling box, thermal insulation cotton is provided between the second cooling box and the incubation box base, and a temperature sensor is provided on the second temperature conduction plate; a handle is provided at the front end of the incubation box upper cover.

[0018] Furthermore, the rocking platform includes a motor arranged at the bottom of the cavity and a crank-connecting rod assembly for transmission connection between the motor and the incubation box base, the crank-connecting rod assembly includes a crank with one end fixed to the output shaft of the motor and a connecting rod with one end rotatably connected to the other end of the crank, and the middle of the bottom rear side of the incubation box base is rotatably connected to the other end of the connecting rod through a connecting plate; the bottom of the cavity is also symmetrically provided with two support seats for supporting the incubation box assembly, and the bottom front side of the incubation box base is provided with two connecting members rotatably connected to the support seats in a one-to-one correspondence.

[0019] Furthermore, the fully automatic protein blotting processing system also includes a water tank arranged in the outer shell, a third cooling box circulated and connected to the water tank through a pipeline, and a refrigerator abutting the surface of the third cooling box, one end of the third cooling box is provided with a water inlet and a water outlet respectively connected to the water tank, and an internal circulation pump is provided in the pipeline connecting the water inlet and the water tank; the water inlet and water outlet of the first cooling box and the second cooling box are respectively connected to the water tank through pipelines, and a first water pump is provided on the pipeline connecting the water tank and the water inlet of the first cooling box, and a second water pump is provided on the pipeline connecting the water tank and the water inlet of the second cooling box.

[0020] Furthermore, the water tank is provided with a liquid level sensor for detecting the liquid level and a temperature sensor for detecting the temperature of the liquid in the water tank.

[0021] Furthermore, the driving device includes a vertical driving device for driving the sample tube to move longitudinally and a horizontal driving device for driving the sample tube and the vertical driving device to move horizontally, and the vertical driving device is provided with a clamping member for clamping the upper end of the sample tube; the vertical driving device is provided with a first travel switch for limiting the up and down travel of the clamping member, and the left and right ends of the horizontal driving device are provided with a second travel switch for limiting the left and right travel of the vertical driving device.

[0022] Furthermore, a base is provided at the bottom of the cavity, and two grooves are provided on the base for respectively accommodating the cleaning liquid box and the waste liquid box, the front end of the groove is open, and at least one spring plate for tightening the cleaning liquid box or the waste liquid box is provided on both sides of the groove, a first magnet is provided at the bottom of the groove, and a second magnet that is magnetically attracted to the first magnet is provided at the bottom of the cleaning liquid box and the waste liquid box; handles are provided at the front end of the cleaning liquid box and the waste liquid box.

[0023] Furthermore, a hand-operated swing assembly for driving the pipette to rotate between a horizontal state and a vertical state is also provided in the cavity, and the hand-operated swing assembly includes two damped rotating parts symmetrically arranged on the left and right and used to clamp the upper end of the pipette, and a rocker arm provided on the outside of one of the damped rotating parts, and the damped rotating part includes a mounting part fixed on the outer shell, a rotating part rotatably arranged on the inner side of the mounting part, and a fixing part fixed on the inner side of the rotating part, a damping part is provided between the rotating part and the mounting part, the upper end of the pipette is clamped between the two fixing parts, and the rocker arm is provided on the outside of one of the mounting parts.

[0024] Furthermore, a touch screen for human-computer interaction and display is provided on one side of the shell. A rotating head that rotates longitudinally is rotatably provided on one side of the shell, and a rotating seat that rotates horizontally is rotatably provided at the lower end of the rotating head. The rear side of the touch screen is fixed on the rotating seat.

[0025] The utility model has the following beneficial effects:

[0026] 1. A sample pipette that can move up and down and left and right between the waste liquid box, the reagent kit component and the incubation box component is used in conjunction with a plunger pump to achieve the purpose of automatic liquid suction and automatic liquid discharge, so that the sample pipette can be used to absorb and discharge the reagents (such as blocking solution, primary antibody and secondary antibody) in the reagent kit component into the antibody incubation tank in the incubation box component for swing incubation; after incubation, the primary antibody and secondary antibody to be recovered are absorbed and discharged back to the corresponding positions in the reagent kit component; then the cleaning liquid is absorbed by the pipette and then passed through the plunger pump and discharged by the sample pipette into the antibody incubation tank to clean the used antibody incubation tank; the liquid after cleaning in the antibody incubation tank is absorbed and discharged into the waste liquid box by the sample pipette, thereby realizing the functions of automatic loading, automatic recovery, automatic cleaning, automatic incubation, etc., with a high degree of automation, ensuring the stability and accuracy of the test data.

[0027] 2. The reagents used in the experiment, such as blocking solution, primary antibody, and secondary antibody, are all proteins, which will slowly denature at room temperature. The entire experimental process takes several hours. If the protein reagents are not temperature-controlled, protein denaturation may affect the experimental results. At the same time, expensive reagents such as primary antibodies and secondary antibodies cannot be reused. Therefore, in the utility model, a cooling box is added to the bottom of the test kit and the incubation box, and the cooling box is used to cool the two, and the temperature of the protein reagents in the two is controlled by the use of a temperature sensor.

[0028] 3. The internal water tank is connected to three pump bodies, and the three pump bodies are used to realize independent control of three water circuits. One is used for self-circulating refrigeration of the liquid in the water tank, the second is used to connect with the reagent box to cool the reagent box, and the third is used to connect with the incubation box to cool the incubation box. The three circuits do not interfere with each other. One or two circuits can be opened for use separately, or all three circuits can be used together.

[0029] Additional aspects and advantages of the present invention will be partially given in the following description, which will become apparent from the following description or be understood through the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] The drawings described herein are used to provide a further understanding of the present invention, constitute a part of the present application, and do not constitute an improper limitation of the present invention. In the drawings:

[0031] Figure 1 Schematic diagram of a fully automated protein blotting processing system in the embodiment;

[0032] Figure 2 This is a schematic diagram of the fully automatic protein blotting processing system in the embodiment after the outer shell is removed;

[0033] Figure 3This is a schematic diagram of the interior of the reagent kit assembly, incubation box assembly, rocking platform, waste liquid box and cleaning liquid box after assembly in the embodiment;

[0034] Figure 4 for Figure 3 Schematic diagram of the mid-dorsal side;

[0035] Figure 5 This is a schematic diagram of the disassembly of the incubation box assembly and the rocking platform after assembly in the embodiment;

[0036] Figure 6 Schematic diagram of the kit components in the embodiment;

[0037] Figure 7 A schematic diagram of the kit assembly in the embodiment with the upper cover of the kit opened;

[0038] Figure 8 Schematic diagram of the decomposition of the kit components in the embodiment;

[0039] Fig. 9 This is a schematic diagram of the disassembly of the waste liquid box and the cleaning liquid box when they are assembled with the base in the embodiment;

[0040] Fig.10 A schematic diagram of a base in an embodiment;

[0041] Fig.11 is a cross-sectional view of a waste liquid box or a cleaning liquid box in an embodiment;

[0042] Fig.12 Schematic diagram of the pipeline connection between the sample adding tube, the liquid pipette and the plunger pump in the embodiment;

[0043] Fig.13 This is a schematic diagram of the sample adding tube and the driving device after being assembled in the embodiment;

[0044] Fig.14 This is a schematic diagram of the assembly of the pipette and the hand swing assembly in the embodiment;

[0045] Fig.15 This is a schematic diagram of the embodiment in which the pipette is rotated to a horizontal state;

[0046] Fig.16 Schematic diagram of the pipe connection between the water tank, water pump and cooling box in the embodiment

[0047] Fig.17 It is a schematic diagram of the embodiment in which the panel and the vertical moving device are assembled and in an unopened state;

[0048] Fig.18 It is a schematic diagram of the embodiment in which the panel and the vertical moving device are assembled and in an unopened state;

[0049] Fig.19It is a schematic diagram of the touch screen after being fixed to the rotating head and the rotating base in the embodiment. DETAILED DESCRIPTION

[0050] In order to more fully understand the technical content of the present invention, the present invention will be further introduced and explained in conjunction with the accompanying drawings and specific embodiments below; it should be noted that descriptions such as "first" and "second" in the text are used to distinguish different components, etc., and do not represent the order of precedence, nor do they limit the "first" and "second" to different types.

[0051] The technical solutions in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all of the embodiments; based on the embodiments of the utility model, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the utility model.

[0052] Example

[0053] like Figures 1 to 19 As shown, the fully automatic protein blotting processing system shown in this embodiment includes a shell 1, a panel 2, and a reagent kit component 3, an incubation box component 4, a rocking platform, a pipetting component, a waste liquid box 5, a cleaning liquid box 6 and a pipette 7 in the shell 1. A cavity is provided inside the shell 1 for accommodating the above components. An opening is provided on the front side of the shell 1 to expose the internal components. The panel 2 can be opened and closed and is arranged at the front end of the shell 1 and is used to cover or open the opening; the reagent kit component 3 is arranged at the bottom of the cavity and is used to separate and accommodate a variety of different reagents. The conventionally accommodated reagents include blocking solution, primary antibody and secondary antibody; the incubation box component 4 is arranged on the right side of the reagent kit component 3, and at least two antibody incubation grooves 44 are arranged on the incubation box component 4 for antibody incubation; the rocking platform is arranged at the bottom of the cavity and is used to drive the incubation box component to tilt, rotate and swing back and forth, that is, the incubation box component 4 can be moved within a certain angle range The waste liquid box 5 is arranged on the left side of the reagent kit assembly 7 or the right side of the incubation box assembly and is used to collect waste liquid; the pipetting assembly includes a sample tube 8 arranged at the upper end of the cavity and used to absorb the reagent in the reagent kit assembly 3 and a driving device for driving the sample tube 8 to move in the longitudinal and horizontal directions, so that the sample tube 8 can move back and forth between the waste liquid box 5, the reagent kit assembly 3 and the incubation box assembly 4; the cleaning liquid box 6 is arranged on the left side of the waste liquid box 5 and is used to accommodate the cleaning liquid; the lower end of the pipette 7 is inserted into the cleaning liquid box 6 and is used to absorb the cleaning liquid, and the upper end is connected to the upper end of the sample tube 8 through the plunger pump 9, and the pipelines between the plunger pump 9 and the pipette 7 and the sample tube 8 are provided with electromagnetic valves 71 for controlling the on-off of the pipelines at both ends, and the plunger pump is double-headed for output and input, and its two ends are respectively connected to the electromagnetic valves 71 (such as Fig.13 As shown), it is used to control the aspiration operation and discharge operation between the aspiration tube and the sample adding tube respectively, and can make the aspiration tube 7 and the sample adding tube 8 communicate with each other.

[0054] In the above, a sample loading tube that can move up and down and left and right between the waste liquid box, the reagent kit component and the incubation box component is used, and is used in conjunction with a plunger pump to achieve the purpose of automatic liquid suction and automatic liquid discharge, so that the sample loading tube can be used to absorb and discharge the reagents in the reagent kit component (such as blocking solution, primary antibody and secondary antibody) into the antibody incubation tank in the incubation box component for swing incubation; after incubation, the primary antibody and secondary antibody to be recovered are absorbed and discharged back to the corresponding positions in the reagent kit component; then the cleaning liquid is absorbed by the pipette and then passed through the plunger pump and discharged by the sample loading tube into the antibody incubation tank to clean the used antibody incubation tank; the liquid after cleaning in the antibody incubation tank is absorbed and discharged into the waste liquid box by the sample loading tube, thereby realizing the functions of automatic loading, automatic recovery, automatic cleaning, automatic incubation, etc., with a high degree of automation, ensuring the stability and accuracy of the test data.

[0055] In one embodiment, if Fig.13 As shown, a liquid circuit monitoring sensor 72 for detecting the flow state of the liquid in the pipeline is provided on the pipeline between the plunger pump 9 and the pipette 7 and the sample adding tube 8, so as to control the suction and discharge of the liquid by the pipette and the sample adding tube by turning on and off the solenoid valve. The liquid circuit monitoring sensor can monitor the state of the liquid in the pipeline in real time, so as to determine and know whether the suction and discharge of the liquid have been completed; the liquid circuit monitoring sensor is a water flow sensor or a liquid flow meter sensor, etc.

[0056] In one embodiment, if Figure 17 to Figure 18As shown, the fully automatic protein blotting processing system also includes a vertical moving device for driving the panel 2 to slide up and down and open, the vertical moving device includes two transmission belts 20 arranged vertically on both sides of the front end of the shell 1, a transmission shaft 21 rotatably arranged inside the shell 1 through a bearing and connected to the two transmission belts 20, and a motor 22 for driving the transmission shaft 21 to be connected to the transmission. The front end of the shell 1 is also provided with two guide shafts 23 respectively located in front of the two transmission belts 20, a slider 24 is slidably provided on the guide shaft 23, one side of the slider 24 is fixedly connected to the transmission belt 20, and the inner upper end or lower end of the panel 2 is connected to the two transmission belts 20. The slider 24 on the guide shaft 23 is fixedly connected; a first synchronous pulley 25 is provided at both ends of the transmission shaft 21, and two second synchronous pulleys 26 are rotatably provided in the housing 1 and are respectively located below the first synchronous pulley 25, and the transmission belt 20 is wound around the first synchronous pulley 25 and the second synchronous pulley 26; a driven gear 27 is also provided on the transmission shaft 21, and a driving gear 28 meshing with the driven gear 27 is provided on the output shaft of the motor 22; in this structure, the motor 22 is used to drive the transmission shaft 21 to rotate, thereby driving the transmission belt 20 to rotate up and down and the slider to slide up and down, thereby driving the panel 2 to be lifted up and down to open or close.

[0057] Preferably, a tensioning wheel 29 is rotatably provided in the housing and is located on one side of the transmission belt 20 to tension the transmission belt.

[0058] In one embodiment, in order to prevent the lifting door from causing dangerous behaviors such as pinching hands during the up and down movement, the motor 22 is preferably a double-headed reduction DC motor, one end of which is connected to the transmission shaft, and the other end is connected to the encoder 221. When the panel descends and closes, it can stop immediately if there is an object blocking it from below, preventing hands or objects from being pinched.

[0059] In one embodiment, if Figures 6 to 8As shown, the reagent kit assembly 3 includes a reagent kit base 30, a reagent kit upper cover 31 rotatably mounted on the upper end of the reagent kit base 30, and three reagent kits 32 arranged between the reagent kit base 30 and the reagent kit upper cover 31 and used to respectively accommodate different reagents. The three reagent kits 32 are respectively used to accommodate a blocking solution, a primary antibody, and a secondary antibody. The reagent kit upper cover 31 is provided with three first through holes 33 for the sample tubes 8 to pass through and corresponding to the three reagent kits 32 one by one, which are used to absorb the internal liquid or discharge the liquid after the sample tubes 8 pass through. The interior of the reagent kit base 30 is provided with a first cooling box 34 and a first temperature conducting plate 35 disposed on the upper end of the first cooling box 34. The first cooling box 34 is used to cool the reagent kit 32. The reagent kit 32 is disposed on the first temperature conducting plate 35, which can better conduct temperature. The temperature of the reagent box 32 is adjusted to the liquid in the three reagent boxes 32. The first cooling box 34 is provided with a water inlet 13 and a water outlet 14 for connecting with an external water supply device to form a circulating water cooling structure. A thermal insulation cotton 36 is provided between the first cooling box 34 and the reagent box base 30 for thermal insulation to reduce excessive loss of useless temperature. At the same time, after the reagent box cover 31 is closed, the loss of internal temperature can be reduced to better maintain the temperature balance in the reagent box. A temperature sensor 37 for detecting its temperature is provided on the first temperature conduction plate 35 to monitor the temperature change in real time to ensure that the protein reagent in the reagent box is temperature controlled, and the temperature can be as low as about 4°. The reagent box cover 31 and the front end of the reagent box 32 are both provided with handles 38 for conveniently flipping open the reagent box cover 31 and taking out or putting in the reagent box 32.

[0060] In one embodiment, if Figure 5As shown, the incubation box assembly 4 includes an incubation box base 40, an incubation box upper cover 41 which is rotatably mounted on the upper end of the incubation box base 40, and two incubation boxes 42 which are arranged left and right between the incubation box base 40 and the incubation box upper cover 41, that is, the incubation box upper cover 41 can be flipped open to expose the incubation box 42 inside, and the incubation box upper cover 41 is provided with a plurality of second through holes 43 for the sample tube 8 to pass through, which are used to absorb the internal liquid or discharge the liquid after the sample tube 8 passes through, and the upper end of the incubation box 42 is recessed with at least one antibody incubation groove 44 which is arranged left and right; a second cooling box 45 and a second temperature conducting plate 46 which is arranged on the upper end of the second cooling box 45 are provided inside the incubation box base 40, and the second cooling box 45 is used to cool the incubation box 42. 2 is arranged on the second temperature conducting plate 46, which can better conduct the temperature to the liquid in the incubation box 42. The second cooling box 45 is provided with a water inlet and a water outlet, which is used to connect with the external water supply device to form a circulating water cooling structure; a thermal insulation cotton 36 is arranged between the second cooling box 45 and the incubation box base 40, which is used for thermal insulation and reducing the excessive loss of useless temperature. At the same time, after the incubation box cover 41 is closed, the loss of internal temperature can also be reduced, and the temperature balance in the incubation box can be better maintained; a temperature sensor is arranged on the second temperature conducting plate 46 to monitor the temperature change in real time to ensure the temperature control of the protein reagent in the test kit, and the temperature can be as low as about 4°; a handle 38 is arranged at the front end of the incubation box cover 41, which is convenient for flipping and opening the incubation box cover 41.

[0061] In one embodiment, the incubation box 42 is a protein blotting membrane incubation / washing container. The incubation box 42 is preferably made of plastic injection molding such as PP or PC. The size is determined according to the size of the standard polyacrylamide gel electrophoresis tank and the actual application. There are 3 specifications, namely a 90*70mm incubation box with a single-grid antibody incubation tank, a 90*30mm incubation box with a double-grid antibody incubation tank, and a 90*16mm incubation box with an antibody incubation tank (the double grid is divided at a ratio of 1:2, and the three grids are divided at a ratio of 1:1:1). Other sizes and specifications are also possible. When two incubation boxes 42 are used, the three different specifications of incubation boxes can be randomly matched according to needs. It can be a combination of two incubation boxes of the same specification or a combination of two incubation boxes of different specifications. Therefore, there are a total of 9 different matching methods to install the incubation boxes to meet different experimental needs.

[0062] In one embodiment, if Figure 2 and Fig.16As shown, the reagents used in the experiment, such as blocking solution, primary antibody, and secondary antibody, are all proteins, which will slowly denature at room temperature. The entire experimental process lasts for several hours. If the protein reagents are not temperature-controlled, protein denaturation may affect the experimental results. At the same time, expensive reagents such as primary antibodies and secondary antibodies cannot be reused. Therefore, a cooling box for cooling is provided in the reagent kit assembly and the incubation box assembly. In order to supply coolant to the cooling box, the fully automatic protein blotting processing system also includes a water tank 10 arranged in the outer shell 1, a third cooling box 11 circulatedly connected to the water tank 10 through a pipeline, and a refrigerator 12 abutting against the bottom surface of the third cooling box 11. One end of the third cooling box 11 is provided with a water inlet and a water outlet respectively connected to the water tank 10, and the pipeline connecting the water inlet to the water tank 10 is provided with an internal circulation A separate circulation loop is formed between the inner circulation pump 15 (preferably a water pump), the third cooling box 11, the inner circulation pump 15 and the water tank 10, and the coolant (preferably alcohol or ethylene glycol coolant) in the water tank 10 can be pumped into the third cooling box 11 by the inner circulation pump 15, and the liquid entering the third cooling box 11 is cooled by the refrigerator 12. After cooling, the cooled liquid is discharged back into the water tank. At this time, the liquid in the water tank is cooled. After circulating cooling in this way, the temperature can be reduced to below zero degrees; the first cooling box 34 and the third The water inlet and the water outlet of the second cooling box 45 are respectively connected to the water tank 10 through pipes, and the first water pump 16 is provided on the pipe connecting the water tank 10 with the water inlet of the first cooling box 34. At this time, a separate circulation loop is formed between the first cooling box 34, the first water pump 16 and the water tank 10, and the liquid in the water tank 10 can be pumped into the first cooling box 34 by the first water pump 16, and the first cooling box 34 is circulated to cool down, and then the temperature of the corresponding reagent in the test kit can be reduced by temperature conduction; the pipe connecting the water tank 10 with the water inlet of the second cooling box 45 A second water pump 17 is provided on the road, and a separate circulation loop is formed between the second cooling box 45, the second water pump 17 and the water tank 10. The liquid in the water tank 10 can be pumped into the second cooling box 45 by the second water pump 17, and the second cooling box 45 is circulated to cool down, and then the temperature of the corresponding reagents in incubation boxes of different specifications can be reduced by temperature conduction; here, three independent circulation water circuits are formed between the water tank and the three cooling boxes, and the three circuits do not interfere with each other. One or two circuits can be opened for use separately, or all three circuits can be used together.

[0063] In one embodiment, if Fig.16 As shown, the water tank 10 is provided with a liquid level sensor 161 for detecting the liquid level and a temperature sensor 37 for detecting the temperature of the liquid in the water tank 10, which can read the liquid level and temperature in the water tank in real time. When the liquid in the water tank is insufficient, liquid can be added into the water tank to maintain the required cooling function.

[0064] In one embodiment, if Figures 4 to 5 As shown, the rocking platform includes a motor 22 arranged at the bottom of the cavity and a crank-connecting rod assembly that transmits and connects the motor 22 and the incubation box base 40. The crank-connecting rod assembly includes a crank 18 fixed at one end to the output shaft of the motor 22 and a connecting rod 19 that is rotatably connected to the other end of the crank 18 at one end. The middle of the bottom rear side of the incubation box base 40 is rotatably connected to the other end of the connecting rod 19 through a connecting plate 47; two support seats 48 for supporting the incubation box assembly 4 are symmetrically provided at the bottom of the cavity, and two support seats 48 that are rotatably corresponding to the support seats 48 are provided at the front side of the bottom of the incubation box base 40. The connected connecting piece 49, that is, under the drive of the motor 22, the connecting rod 19 makes a reciprocating motion along the arc, driving the entire incubation box assembly to flip over repeatedly, that is, the incubation box base 40 is tilted and rotated up and down around the support seat 48. When the incubation box is swung to a horizontal state, it is convenient to install or remove the incubation box. At the same time, the motor can use a stepping motor or add a sensor. When the incubation box assembly 4 tilts backward at the maximum angle, the liquid in the incubation box is tilted and concentrated to the rear end of the incubation box, which is convenient for the sample tube to absorb the internal reagent / waste liquid, utilizing the working principle of the crank connecting rod assembly.

[0065] In one embodiment, the rocking platform also includes a U-shaped sensor 191 disposed on one side of the connecting rod 19. When the incubation box is swung to a horizontal position, the connecting rod can be detected by the U-shaped sensor 221. At this time, it can be set to the initial position to ensure that the incubation box assembly 4 is in a horizontal position.

[0066] In one embodiment, if Fig.13 As shown, the driving device includes a vertical driving device 81 for driving the sample tube 8 to move in the longitudinal direction and a horizontal driving device 82 for driving the sample tube 8 and the vertical driving device 81 to move in the horizontal direction. The sliding member (such as a slider or a lead screw nut) of the vertical driving device 81 is provided with a clamping member 83 for clamping the upper end of the sample tube 8; the vertical driving device 81 is provided with a first travel switch 84 for limiting the up and down travel of the clamping member 83, and the left and right ends of the horizontal driving device 82 are provided with a second travel switch 85 for limiting the left and right travel of the vertical driving device 81.

[0067] In one embodiment, both the vertical drive device and the horizontal drive device may adopt a screw drive mechanism, and may also adopt a belt drive mechanism or a cylinder drive mechanism, etc. The screw drive mechanism, belt drive mechanism and cylinder drive mechanism are all conventional drive mechanisms in the existing art and will not be described in detail here.

[0068] In one embodiment, if Figures 9 and 10As shown, a base 50 is also provided at the bottom of the cavity, and two grooves 51 are concavely provided on the base 50 for accommodating the cleaning liquid box 6 and the waste liquid box 5 respectively. The front end of the groove 51 is open, which is convenient for the cleaning liquid box 6 and the waste liquid box 5 to slide into or slide out. At least one spring piece 52 is provided on both sides of the groove 51 to press against the cleaning liquid box 6 or the waste liquid box 5, which can be clamped when the cleaning liquid box 6 and the waste liquid box 5 are inserted; a first magnet 53 is provided at the bottom of the groove 51, and a second magnet 54 that is magnetically attracted to the first magnet 53 is provided at the bottom of the cleaning liquid box 6 and the waste liquid box 5. When inserted into place, the upper and lower magnets are attracted, and it will be clear to the senses that they have been inserted into place at this time; a handle 38 is provided at the front end of the cleaning liquid box 6 and the waste liquid box 5. When taking out, the handle 38 is pulled out by one hand. When most of the cleaning liquid box 6 or the waste liquid box 5 is out, the other hand holds it up to complete the removal process.

[0069] In one embodiment, if Fig.11 As shown, the inner bottoms of the cleaning liquid box and the waste liquid box are both tilted backwards, so that the liquid is always concentrated at the rear end of the box, which is convenient for sucking and discharging the liquid cleanly.

[0070] In one embodiment, if Figure 14 to Figure 15 As shown, a hand swing assembly 90 for driving the pipette 7 to rotate between a horizontal state and a vertical state is also provided in the cavity. The hand swing assembly 90 includes two symmetrically arranged damped rotating parts for clamping the upper end of the pipette 7 and a rocker 91 provided on the outside of one of the damped rotating parts. The damped rotating part includes a mounting part 92 fixed on the outer shell 1, a rotating part 93 rotatably arranged on the inner side of the mounting part 92, and a fixing part 94 fixed on the inner side of the rotating part 93. The rotating part 93 can rotate 360 ​​degrees relative to the mounting part 92. The upper end of the pipette 7 is clamped between the two fixing parts 94, and the rocker 91 is provided on the outer side of one of the mounting parts 92. After grasping the rocker 91 by hand, the hand swing assembly can be rotated upward by 90 degrees, so that the pipette fixed thereon rotates upward at the same time. The pipette 7 rotates from a vertical state to a horizontal state, so that it leaves the cleaning liquid box. At this time, the cleaning liquid box can be drawn out to perform liquid adding or pouring operations, such as Fig.15 When the liquid is added, the cleaning liquid box is pushed in, and the rocker 91 is manually swung to rotate the pipette 7 from the horizontal state to the vertical state. At this time, the pipette 7 is inserted into the liquid in the cleaning liquid box 6, as shown. Fig.14 shown.

[0071] In one embodiment, a damping member 98 is provided between the rotating member 93 and the mounting member 92, and the damping torque of the damping member can also be adjusted by a nut at the rear end, so that the pipette can remain stable when rotating to any state.

[0072] In one embodiment, if Figures 1 to 19As shown, a touch screen 95 for human-computer interaction and display is also provided on one side of the housing 1, and the touch screen 95 can be manually rotated in two levels. The first rotation is the rotation of the touch screen 95 in the horizontal direction, which is a switching process of the touch screen 95 rotating from the side to the front; the second rotation is the rotation of the touch screen 95 in the vertical direction, which realizes the upward rotation of the touch screen from a vertical state to an inclined or horizontal state; in order to achieve the purpose of two-level rotation, a rotating head 96 that rotates in the longitudinal direction is rotatably provided on one side of the housing 1, that is, the rotating head 96 can rotate in the vertical plane, and a rotating seat 97 that can rotate in the horizontal direction is rotatably provided at the lower end of the rotating head 96, that is, the rotating seat 97 can rotate in the horizontal plane, and the rear side of the touch screen 95 is fixed on the rotating seat, so that it can be rotated left and right and up and down to adjust the touch screen to a comfortable screen display viewing angle.

[0073] In other embodiments, the thermal insulation cotton is preferably made of polyurethane.

[0074] In other embodiments, both the sample adding tube and the liquid pipette are capillaries.

[0075] The technical solutions provided by the embodiments of the present invention are introduced in detail above. Specific examples are used in this article to illustrate the principles and implementation methods of the embodiments of the present invention. The description of the above embodiments is only applicable to help understand the principles of the embodiments of the present invention. At the same time, for those skilled in the art, according to the embodiments of the present invention, there will be changes in the specific implementation methods and application scopes. In summary, the content of this specification should not be understood as a limitation on the present invention.

Claims

1. A fully automatic protein blotting processing system, characterized in that: include: A shell having a cavity inside and an opening at the front side of the shell; A panel, which is closable and arranged at the front end of the housing and is used to cover or open the opening; A reagent kit assembly, which is arranged at the bottom of the chamber and is used to separate and accommodate a plurality of different reagents; An incubation box assembly, which is arranged on the right side of the test kit assembly, and at least two antibody incubation tanks are arranged on the incubation box assembly; A swing platform, which is arranged at the bottom of the cavity and is used to drive the incubation box assembly to tilt, rotate and swing forward and backward; A waste liquid box, which is arranged on the left side of the reagent kit assembly or the right side of the incubation box assembly and is used to collect waste liquid; A pipetting assembly, the pipetting assembly comprising a sample loading tube disposed at the upper end of the chamber and used to absorb the reagent in the reagent kit assembly, and a driving device used to drive the sample loading tube to move in the longitudinal and horizontal directions, so that the sample loading tube can move back and forth between the waste liquid box, the reagent kit assembly and the incubation box assembly; A cleaning liquid box, which is arranged on the left side of the waste liquid box and is used to contain the cleaning liquid; A pipette, the lower end of which is inserted into the cleaning liquid box, the upper end of which is communicated with the upper end of the sample adding tube through a plunger pump, and electromagnetic valves are arranged between the plunger pump and the pipette and the sample adding tube.

2. The fully automatic protein blotting processing system according to claim 1, characterized in that: The cam is an electrically conductive member which is actuated by the motion of a user pushing the cam forward along the guide rail, the latter being an electrically conductive member which is actuated by the motion of a user pushing the cam forward along the guide rail.

3. The fully automatic protein blotting processing system according to claim 1, characterized in that: The reagent kit assembly includes a reagent kit base, a reagent kit upper cover rotatably mounted on the upper end of the reagent kit base, and at least three reagent kits arranged between the reagent kit base and the reagent kit upper cover and used to respectively accommodate different reagents. The reagent kit upper cover is provided with a plurality of first through holes for sample tubes to pass through. A first cooling box and a first temperature conduction plate disposed on the upper end of the first cooling box are disposed inside the reagent kit base. The reagent kit is disposed on the first temperature conduction plate. A water inlet and a water outlet are disposed on the first cooling box. Insulation cotton is disposed between the first cooling box and the reagent kit base. A temperature sensor is disposed on the first temperature conduction plate. The reagent kit upper cover and the front end of the reagent kit are both provided with handles.

4. The fully automatic protein blotting processing system according to claim 3, characterized in that: The incubation box assembly includes an incubation box base, an incubation box cover that is rotatably mounted on the upper end of the incubation box base, and at least two incubation boxes that are arranged left and right between the incubation box base and the incubation box cover. The incubation box cover is provided with a plurality of second through holes for sample tubes to pass through, and the upper end of the incubation box is recessed with at least one antibody incubation groove that is arranged left and right; a second cooling box and a second temperature conduction plate that is arranged on the upper end of the second cooling box are provided inside the incubation box base, the incubation box is provided on the second temperature conduction plate, a water inlet and a water outlet are provided on the second cooling box, thermal insulation cotton is provided between the second cooling box and the incubation box base, and a temperature sensor is provided on the second temperature conduction plate; a handle is provided at the front end of the incubation box cover.

5. The fully automatic protein blotting processing system according to claim 4, characterized in that: The rocking platform includes a motor arranged at the bottom of the cavity and a crank-connecting rod assembly for transmission connection between the motor and the incubation box base, the crank-connecting rod assembly includes a crank with one end fixed to the output shaft of the motor and a connecting rod with one end rotatably connected to the other end of the crank, and the middle of the bottom rear side of the incubation box base is rotatably connected to the other end of the connecting rod through a connecting plate; two support seats for supporting the incubation box assembly are also symmetrically provided at the bottom of the cavity, and two connecting members rotatably connected to the support seats in a one-to-one correspondence are provided on the front side of the bottom of the incubation box base.

6. The fully automatic protein blotting processing system according to claim 4 or 5, characterized in that: It also includes a water tank arranged in the outer shell, a third cooling box connected to the water tank for circulation through a pipeline, and a refrigerator abutting the surface of the third cooling box, one end of the third cooling box is provided with a water inlet and a water outlet connected to the water tank respectively, and an internal circulation pump is provided in the pipeline connecting the water inlet and the water tank; the water inlet and the water outlet of the first cooling box and the second cooling box are connected to the water tank through pipelines respectively, and a first water pump is provided on the pipeline connecting the water tank and the water inlet of the first cooling box, and a second water pump is provided on the pipeline connecting the water tank and the water inlet of the second cooling box; a liquid level sensor for detecting the liquid level and a temperature sensor for detecting the temperature of the liquid in the water tank are provided in the water tank.

7. The fully automatic protein blotting processing system according to claim 1, characterized in that: The driving device includes a vertical driving device for driving the sample adding tube to move in the longitudinal direction and a horizontal driving device for driving the sample adding tube and the vertical driving device to move in the horizontal direction. The vertical driving device is provided with a clamping member for clamping the upper end of the sample adding tube; the vertical driving device is provided with a first travel switch for limiting the up and down travel of the clamping member, and the left and right ends of the horizontal driving device are both provided with a second travel switch for limiting the left and right travel of the vertical driving device.

8. The fully automatic protein blotting processing system according to claim 1, characterized in that: A base is also provided at the bottom of the cavity, and two grooves are concavely provided on the base for respectively accommodating the cleaning liquid box and the waste liquid box, the front end of the groove is open, and at least one spring piece for tightening the cleaning liquid box or the waste liquid box is provided on both sides of the groove, a first magnet is provided at the bottom of the groove, and a second magnet that is magnetically attracted to the first magnet is provided at the bottom of the cleaning liquid box and the waste liquid box; handles are provided at the front end of the cleaning liquid box and the waste liquid box.

9. The fully automatic protein blotting processing system according to claim 1, characterized in that: The cavity is also provided with a hand-operated assembly for driving the pipette to rotate between a horizontal state and a vertical state, the hand-operated assembly includes two symmetrically arranged damped rotating parts for clamping the upper end of the pipette and a rocker provided on the outside of one of the damped rotating parts, the damped rotating part includes a mounting part fixed on the outer shell, a rotating part rotatably provided on the inner side of the mounting part and a fixing part fixed on the inner side of the rotating part, a damping part is provided between the rotating part and the mounting part, the upper end of the pipette is clamped between the two fixing parts, and the rocker is provided on the outside of one of the mounting parts.

10. The fully automatic protein blotting processing system according to claim 1, characterized in that: A touch screen for human-computer interaction and display is also provided on one side of the shell. A rotating head that rotates longitudinally is rotatably provided on one side of the shell, and a rotating seat that rotates horizontally is rotatably provided at the lower end of the rotating head. The rear side of the touch screen is fixed on the rotating seat.

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