Device for measuring toxicity of fruit flies
By designing a fruit fly virility measurement device including a tank body, a lid, a Baspsteel pipette suction capsule, a partition plate and a mesh sleeve, the existing device has solved the problem of low fault tolerance and low experimental efficiency when determining male and female concentrations separately and multi-gradient concentrations, and achieved efficient and accurate virility measurement.
Patent Information
- Application Number
- CN202421518429.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-30
- Publication Date
- 2025-04-22
- Estimated Expiration
- 2034-06-30
AI Technical Summary
When the existing fruit fly virility measurement device performs separate measurement of male and female concentration and multi-gradient concentration, the fault tolerance rate is low, and the experimental efficiency and space utilization rate are not high.
A fruit fly virility determination device is designed, including a tank body, a lid, a Basp pipette suction capsule, a partition plate and a mesh sleeve. By setting a Pasteur pipette suction capsule on the lid, the contact between fruit flies and the agent is reduced; by setting a partition plate in the tank body to separate it into several feeding areas, the determination of male and female and the determination of multi-gradient concentrations is achieved; by setting up a worm mouth and a mesh sleeve at the bottom of the tank body, the fault tolerance and experimental efficiency are improved.
The device can accurately measure the gastric toxicity of the agent, improve the fault tolerance and experimental efficiency, and reduce the test footprint and cleaning time.
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Figure CN222776808U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of insect breeding devices, in particular to a fruit fly toxicity determination device. Background Art
[0002] Fruit flies are a general term for insects of the family Tephritidae in the order Diptera. They are a type of pest that seriously harms fruit trees and vegetable production in my country. Among them, fruit flies are the most serious, including the citrus fruit fly, citrus fruit fly, citrus fruit fly, pumpkin fruit fly, melon fruit fly, guava fruit fly, etc. After mating, female insects lay eggs inside vegetables and fruits, and the larvae feed on the flesh of vegetables and fruits, causing them to rot and deteriorate, fall off prematurely, and lose their commercial value. At present, the control plan for fruit flies is diverse, mainly based on chemical pesticides, combined with the use of sex attractants, food attractants, release of parasitic wasps, and garden cleaning. Among the methods for determining the toxicity of chemical agents, the drug film method and the drip method are mainly used to determine the contact effect of the agent. Relatively speaking, the method for determining the toxicity of stomach poisons for insects with licking mouthparts is more accurate.
[0003] The existing device for determining the toxicity of the stomach poison of fruit flies can refer to the patent "A method for determining the toxicity of the stomach poison of the orange fruit fly using a simple device" (CN104770340B). The device for determining the toxicity of the stomach poison of the orange fruit fly in this patent includes a cotton ball, a pipette tip, a disposable plastic cup, a gauze and other structures. The device has a simple structure, can reduce the contact between the orange fruit fly and the agent, avoid the interference of the contact killing effect, and can accurately measure the toxicity of the stomach poison. However, the device needs to make multiple devices when performing separate male and female measurements and multi-gradient concentration measurements. The area occupied during the experiment is large, and it takes a lot of time to clean them one by one after the experiment. In addition, the insect entry setting of the device is not perfect, the insect opening is too large and there is no obstruction, which easily causes the insects to fly away, and if the insects of the other sex are accidentally mixed in when the male and female are measured separately, they cannot be taken out, and the whole cup can only be re-inserted, which reduces the efficiency of the experiment. Utility Model Content
[0004] The utility model aims to provide a fruit fly toxicity determination device, solve the problem of low fault tolerance of the existing fruit fly toxicity determination device, and improve space utilization and experimental efficiency.
[0005] The specific technical solutions are as follows:
[0006] A fruit fly toxicity determination device comprises a tank body, a cover, a Pasteur pipette suction bag, a partition plate and a gauze cover.
[0007] The lid is arranged on the top of the tank body, and the lid can rotate relative to the tank body. The lid is provided with at least two insertion holes. The Pasteur pipette suction bag is detachably inserted at the insertion holes. One end of the Pasteur pipette suction bag is sealed and the other end is open. When in use, the Pasteur pipette suction bag is squeezed to inhale the liquid medicine, and then inserted into the insertion hole. The open end of the Pasteur pipette suction bag extends into the tank body.
[0008] The tank body is a hollow cylinder with an opening at the top, a vent hole is provided at the top of the tank body, an insect placing and taking-out opening is provided at one side of the bottom of the tank body, and the insect placing and taking-out opening is provided with the gauze sleeve; a partition plate is provided in the tank body, the partition plate divides the tank body into at least two insect feeding areas, and the partition plate can rotate relative to the tank body with the cover. The partition plate can be directly fixed on the cover, or can be installed through a rotating shaft or the like.
[0009] Preferably, each insect feeding area is provided with 1-3 Pasteur straw suction sacs.
[0010] Preferably, the Pasteur pipette suction bag is a 1ml disposable plastic rubber-tipped dropper with the pipette part cut off and the suction bag part retained for use, the suction bag has an outer diameter of 12mm, a length of 35mm, and a lower pipette opening width of 4mm. The width of the pipette opening is smaller than the diameter of the other parts of the suction bag, that is, the opening is retracted.
[0011] Preferably, a hole for a socket is punched on the top of the cover using an electric soldering iron punch, and the diameter of the hole is about 1.2 cm.
[0012] Preferably, the tank body is a transparent plastic tank.
[0013] Preferably, there are 3-6 insect feeding areas.
[0014] Preferably, a column is fixed at the center of the cover, and vertical slots are evenly spaced along the circumferential direction on the column, and one side of the partition plate is detachably inserted on the column. The installation position of the partition plate can be rotated as needed to divide the required number of insect rearing areas.
[0015] Preferably, the insect release opening is rectangular, with length*width=8cm*9cm.
[0016] Preferably, the specification of the gauze bag is: length*width=40cm*20cm, the mesh number of the gauze bag is 100 mesh, one end of the gauze bag is connected to the insect release opening with a glue gun, and the end away from the tank body is fixed with a rubber band to prevent the insects from flying away.
[0017] Preferably, the tank body is transparent and the partition is transparent, which facilitates observation of mortality rate.
[0018] Preferably, the diameter of the vent hole is 1 mm.
[0019] Compared with the existing technology, the utility model has the following beneficial effects:
[0020] 1. The utility model provides a Pasteur pipette on the lid. When in use, the Pasteur pipette is squeezed to suck the liquid medicine, and then inserted into the socket. The opening end of the Pasteur pipette suction bag extends into the tank body for fruit flies to suck, which can reduce the interference of contact killing and accurately measure the toxicity of the stomach poison of the medicine. The Pasteur pipette suction bag can store a certain amount of liquid medicine, which can reduce the frequency of adding liquid medicine and will not be polluted by air.
[0021] 2. The utility model divides the tank into several insect feeding areas by setting partitions, which can simultaneously perform male and female measurements and multi-gradient concentration measurements, and can reduce the number of measurement devices and the experimental area. The partitions can be directly fixed on the lid, or a column with a vertical slot can be set in the center of the lid. The number of partitions installed and the installation position of the vertical slots can be selected according to needs, so as to obtain the number of insect feeding areas to meet more experimental needs.
[0022] 3. The utility model provides an insect release opening at the lower part of the tank body, and a gauze cover at the insect release opening. When in use, the fruit flies can be directly placed near the lid of the tank body through the insect release opening, which is convenient for the fruit flies to feed on the liquid in the Pasteur pipette suction bag. Compared with releasing the insects by opening the lid, it is not easy for the fruit flies to fly away. At the same time, if the insects of the other sex are accidentally mixed in when measuring male and female separately, they can be caught through the insect release opening, without taking out the fruit flies in the area and re-catching them, thereby improving the fault tolerance of the device, or when sampling at different toxicity times is required, it is convenient to take samples and continue to measure, thereby enhancing practicality. BRIEF DESCRIPTION OF THE DRAWINGS
[0023] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. In all drawings, similar elements or parts are generally identified by similar reference numerals. In the drawings, the elements or parts are not necessarily drawn according to the actual scale.
[0024] Figure 1 It is a structural schematic diagram of the utility model.
[0025] Figure 2 It is a structural schematic diagram of the lid of the utility model.
[0026] Figure 3 The utility model is a structural schematic diagram of the Pasteur pipette.
[0027] Figure 4 It is a structural schematic diagram of the tank body of the utility model.
[0028] Figure 5 This is a schematic diagram of the installation structure of the partition plate of the utility model.
[0029] Figure 6 This is a schematic diagram of the installation structure of the partition plate in another embodiment of the utility model.
[0030] Among them, the main reference numerals are described as follows:
[0031] 1. Tank body; 2. Lid; 3. Pasteur straw suction bag; 4. Partition plate; 5. Gauze cover; 6. Socket; 7. Vent; 8. Insect release and removal port; 9. Column; 10. Vertical slot. DETAILED DESCRIPTION
[0032] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0033] In the description of the present invention, it should be noted that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "top", "bottom", "top surface", "bottom surface", "inside", "outside", "inside", "outside" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.
[0034] In the description of the present utility model, the meaning of "several" is one or more, the meaning of "more" is more than two, "greater than", "less than", "exceed" etc. are understood to exclude the number itself, and "above", "below", "within" etc. are understood to include the number itself. If there are terms "first", "second" and "third" in the description, they are only used for the purpose of description and distinguishing technical features, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features or implicitly indicating the order of the indicated technical features.
[0035] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected" and "set" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal connection of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances. The following is an explanation of the embodiments of the present invention based on its overall structure.
[0036] Example 1
[0037] like Figure 1 As shown, a fruit fly toxicity determination device comprises a tank body 1, a cover 2, a Pasteur pipette suction bag 3, a partition plate 4 and a gauze cover 5. The utility model provides a Pasteur pipette suction bag 3 on the cover 2 for feeding venom, thereby reducing the contact between fruit flies and venom and accurately measuring the stomach toxicity of the agent. By providing a partition plate 4 in the tank body 1, the tank body 1 is divided into several insect feeding areas, and male and female determinations and multi-gradient concentration determinations can be performed simultaneously, thereby reducing the experimental area.
[0038] Specific, combined Figure 1-2 The lid 2 is disposed on the top of the tank body 1, and at least three sockets 6 are formed on the lid 2, and the Pasteur straw suction bag 3 is detachably inserted in the sockets 6. Figure 3 , one end of the Pasteur straw suction capsule 3 is sealed and the other end is open, and the open end is a closed funnel shape. Preferably, the Pasteur straw suction capsule 3 has an outer diameter of 12mm, a length of 35mm, and an opening width of 4mm. The Pasteur straw suction capsule 3 can be purchased from an existing plastic dropper, the straw part is cut off, and the suction capsule part is retained for use. When in use, after squeezing the Pasteur straw suction capsule 3 to inhale the liquid medicine, it is inserted into the socket 6. The open end of the Pasteur straw suction capsule 3 extends into the tank body 1 for fruit flies to inhale. If the venom test is not performed, the Pasteur straw suction capsule 3 can be inserted upside down at the socket 6, and the socket 6 can be sealed to prevent the fruit flies from escaping, and starvation treatment can be performed, which is simple and practical. The opening width of the Pasteur straw suction capsule 3 is about 4mm, which is convenient for fruit flies to inhale, and the liquid medicine in the Pasteur straw suction capsule 3 will not fall off. If the opening of the Pasteur straw suction bag 3 is too small, after the fruit fly sucks the liquid medicine on the surface, the liquid medicine in the Pasteur straw suction bag 3 is not easy to be replenished downward, which is not conducive to the fruit fly's sucking.
[0039] In other embodiments, several support rods may be provided on the top of the cover 2, wherein the height of the support rods is higher than the height of the Pasteur pipette suction bag 3, so as to facilitate stacking of multiple fruit fly toxicity determination devices and reduce the device footprint.
[0040] Combination Figure 4The tank body 1 is a hollow cylinder with an opening at the top. A vent hole 7 is provided at the upper part of the tank body 1. An insect release opening 8 is provided at one side of the lower part of the tank body 1. The insect release opening 8 is provided with the gauze sleeve 5. The insect release opening 8 is provided at the lower part of the tank body 1 and is equipped with a gauze sleeve 5. Compared with releasing the insects with the lid opened, the insects are not easy to fly away. By providing the insect release opening 8 at the lower part of the tank body 1 and providing the gauze sleeve 5 at the insect release opening 8, when in use, the fruit flies can be directly placed near the lid 2 of the tank body 1 through the insect release opening, so that the fruit flies can feed on the liquid in the Pasteur straw suction bag 3. At the same time, if the insects of the other sex are accidentally mixed in when the male and female are measured separately, they can be caught through the insect release opening 8, without taking out the fruit flies in the area and re-catching them, thereby improving the fault tolerance of the device.
[0041] In other embodiments, a fixing ring is provided at one end of the gauze cover 5 away from the tank body 1, so as to facilitate the operation of taking and releasing the insects.
[0042] Combination Figure 1 The tank body 1 is provided with a partition plate 4, and the partition plate 4 divides the tank body 1 into at least two insect feeding areas. Figure 6 In this embodiment, there are three partition plates 4, which divide the tank body 1 into three insect feeding areas. Two Pasteur straw suction capsules 3 are installed on the lid 2 facing each insect feeding area. Of course, in other embodiments, different numbers of partition plates 4 can also be set, and different numbers of Pasteur straw suction capsules 3 can be matched. In this embodiment, the top of the partition plate 4 is fixed to the lid 2 by glue or the like. When the lid 2 rotates relative to the tank body 1, the Pasteur straw suction capsule 3 and the partition plate 4 also rotate. That is, by rotating the lid 2, different insect feeding areas can be aligned with the insect release port 8 in turn, which is convenient for taking out and putting in fruit flies from different insect feeding areas.
[0043] In other embodiments, the partition may also be fixed in different ways. Figure 6 A column 9 is fixed at the center of the cover 2, and vertical slots 10 are evenly spaced along the circumferential direction on the column 9, and one side of the partition plate 4 is detachably inserted on the column 9. The number of partition plates 4 and the installation positions of the vertical slots 10 are selected as needed to obtain the number of insect feeding areas to meet more experimental requirements.
[0044] In other embodiments, a feeding box is fixed to the lower portion of the partition.
[0045] In this embodiment, the tank body 1 is transparent and the partition is opaque, which is convenient for observing fruit flies in different insect feeding areas.
[0046] The device is used for the determination of the toxicity of the stomach poison of the orange fruit fly. The device is assembled first. Six sockets 6 with a diameter of about 1.2 cm are opened on the lid 2. The partition is cut and fixed to the lid 2 by glue. At the same time, a number of 1 mm diameter pores are opened on the upper part of the tank body 1 by an electric soldering iron, and an insect release opening 8 is also cut out by an electric soldering iron on the lower part of the tank body 1. The insect release opening is length * width = 8cm * 9cm, and the gauze sleeve 5 is fixed at the insect release opening 8. Then the lid 2 is covered on the tank body 1, and the Pasteur pipette suction capsule 3 is inverted first. After preparation, the adult orange fruit fly is rotated into different insect feeding areas through the insect release opening 8 through the partition plate. After starvation for 24 hours, the Pasteur pipette suction capsule 3 is squeezed into the medicine liquid or water and inserted into the socket 6, so that the orange fruit fly can suck the medicine liquid in the Pasteur pipette suction capsule 3. After 24 hours, the mortality rate of each area is recorded. This device can set multiple treatment groups at the same time, which is conducive to the development of the experiment.
[0047] In summary, the utility model provides a Pasteur straw suction bag 3 on the lid 2, and the open end of the Pasteur straw suction bag 3 extends into the tank body 1 for fruit flies to suck, which can reduce the interference of contact killing and accurately measure the stomach poison toxicity of the agent. By providing a partition, the tank body 1 is divided into several insect feeding areas, and male and female measurements and multi-gradient concentration measurements can be performed simultaneously, and the number of measurement devices can be reduced, and the experimental area can be reduced. By providing an insect release opening 8 at the lower part of the tank body 1, and providing a gauze cover 5 at the insect release opening 8, if insects of the other sex are accidentally mixed in when performing male and female measurements, they can be caught through the insect release opening 8, without taking out the fruit flies in the area and re-catching them, thereby improving the fault tolerance of the device, or when sampling at different toxicity times is required, sampling can be conveniently performed and continued measurement can be performed.
[0048] The foregoing descriptions of specific exemplary embodiments of the present invention are for the purpose of explanation and illustration. These descriptions are not intended to limit the present invention to the precise form disclosed, and it is obvious that many changes and variations can be made based on the above teachings. Although an embodiment of the present invention has been shown and described, this specific embodiment is merely an explanation of the present invention and is not a limitation of the present invention. The specific features, structures, materials or characteristics described may be combined in a suitable manner in any one or more embodiments or examples. The purpose of selecting and describing the exemplary embodiments is to explain the specific principles of the present invention and its practical application, so that those skilled in the art can make modifications, substitutions, variations and various selections and changes to the embodiments without creative contribution as needed after reading this specification without departing from the principles and purpose of the present invention, but they are protected by patent law as long as they are within the scope of the claims of the present invention.
Claims
1. A fruit fly toxicity determination device, comprising a tank body, a cover, a Pasteur pipette suction bag, a partition plate and a gauze cover, characterized in that: The lid is arranged on the top of the tank body, and the lid can rotate relative to the tank body. The lid is provided with at least two insertion holes. The Pasteur pipette suction bag is detachably inserted at the insertion holes. One end of the Pasteur pipette suction bag is sealed and the other end is open. When in use, the Pasteur pipette suction bag is squeezed to inhale the liquid medicine, and then inserted into the insertion hole. The open end of the Pasteur pipette suction bag extends into the tank body. The tank body is a hollow cylinder with an opening at the top, a vent hole is provided on the upper part of the tank body, an insect placing and taking-out opening is provided on one side of the lower part of the tank body, and the gauze sleeve is provided at the insect placing and taking-out opening; a partition plate is provided in the tank body, the partition plate divides the tank body into at least two insect feeding areas, and the partition plate can rotate relative to the tank body with the cover.
2. The fruit fly toxicity determination device according to claim 1, characterized in that: Each insect feeding area is provided with 1-3 Pasteur straw suction sacs.
3. The fruit fly toxicity determination device according to claim 2, characterized in that: The Pasteur straw suction bag has an outer diameter of 12 mm, a length of 35 mm, and an opening width of 4 mm.
4. The fruit fly toxicity determination device according to claim 1, characterized in that: The diameter of the socket of the lid is 1.2 cm.
5. The fruit fly toxicity determination device according to claim 1, characterized in that: The tank body is a transparent plastic tank.
6. The fruit fly toxicity determination device according to claim 1, characterized in that: There are 3 to 6 insect feeding areas.
7. The fruit fly toxicity determination device according to claim 6, characterized in that: A column is fixed at the center of the cover, and vertical slots are evenly spaced on the column along the circumferential direction, and one side of the partition plate is detachably inserted on the column.
8. The fruit fly toxicity determination device according to claim 1, characterized in that: The insect placing and taking-in opening is length*width=8cm*9cm.
9. The fruit fly toxicity determination device according to claim 1, characterized in that: The specification of the gauze bag is: length*width=40cm*20cm, the mesh number is 100 meshes, the gauze bag is connected to the insect release and removal opening with hot melt adhesive, and the end away from the tank body is fixed with a rubber band.
10. The fruit fly toxicity determination device according to claim 1, characterized in that: The diameter of the vent hole is 1 mm.
Citation Information
Patent Citations
A method for measuring the virulence of Bactrocera dorsalis stomach poison with a simple device
CN104770340B