Temperature control system for monitoring microbial culture

Abstract

The utility model discloses a temperature control system for monitoring microorganism culture, a corresponding microorganism culture monitoring device comprises a culture box and an incubation frame, the incubation frame is arranged in the culture box, and the incubation frame is used for placing a culture vessel filled with a sample to be detected. The temperature control system comprises: a temperature sensor arranged corresponding to the incubation rack and used for measuring the temperature of the incubation rack to obtain a first temperature detection value of the incubation rack; the heating part is arranged corresponding to the incubation frame and is used for heating the incubation frame; the refrigerating assembly is arranged on the incubator and is used for refrigerating the incubator; and the controller is connected with the temperature sensor, the heating part and the refrigerating assembly and controls the heating part and the refrigerating assembly according to a first temperature detection value obtained by the temperature sensor. According to the incubator, a single incubation frame can be used as a control object to heat the incubation frame, the incubator is used as a control object to integrally refrigerate the incubator, the adaptive environment temperature range is larger, the settable target temperature range is larger, and the temperature uniformity in the incubator can be improved.

Description

Technical Field

[0001] The utility model relates to the biological field, in particular to a temperature control system for monitoring microbial culture. Background Art

[0002] In the detection test of microbial culture monitoring, the tester needs to detect aerobic, facultative or anaerobic microorganisms in the sample to be tested (blood or other usually sterile body fluids) through incubation, shaking and continuous monitoring. In this process, the temperature control accuracy, temperature fluctuation and temperature uniformity have a great influence on the growth state of the microorganisms in the sample, which often determines the accuracy of the monitoring results, resulting in invalidation or failure of the detection test. In addition, different samples require different incubation and culture temperatures. To this end, it is necessary not only to control the fluctuation and uniformity of the test temperature to ensure the smooth progress of the culture monitoring process, but also to be able to accurately set different test temperatures according to the required temperature to adapt to the culture monitoring of various samples. Therefore, it is usually carried out in a laboratory equipped with a constant temperature incubator, or completed by a microbial culture monitoring instrument.

[0003] To achieve microbial culture monitoring that can adapt to various environments and various samples, the following two requirements need to be met:

[0004] 1. Able to be tested normally at ambient temperature outside the laboratory. The ambient temperature of the laboratory is generally 25±5℃, and the ambient temperature outside the laboratory is generally 10℃~40℃;

[0005] 2. The temperature inside the incubator can be set according to the culture and detection temperature of different samples at the ambient temperature outside the laboratory, and the control accuracy, fluctuation and uniformity of the target temperature can be ensured.

[0006] In the prior art, the temperature control scheme adopted is overall heating and natural cooling. A heating tube or a heating wire is used, and air heating is adopted to heat the entire incubator through air. The cooling is enhanced by natural cooling or adding an air cooling system, and the entire incubator is used as a temperature control object for temperature control. For example, the control method can be that when the ambient temperature remains unchanged, the natural cooling caused by heat transfer is stable and unchanged, and the output power of the heating tube or the heating wire is adjusted according to the collected temperature parameters to achieve the purpose of controlling the temperature. However, this temperature control scheme can adapt to a small range of ambient temperatures, a small range of target temperatures that can be set, and the uniformity of temperature control in the incubator is poor. Utility Model Content

[0007] The purpose of the utility model is to provide a temperature control system for microbial culture monitoring, which can make the incubator used for microbial culture monitoring adapt to a larger range of environmental temperatures, a larger range of settable target temperatures, and can improve the temperature uniformity in the incubator used for microbial culture monitoring.

[0008] To achieve the above-mentioned purpose, the utility model provides a temperature control system for microbial culture monitoring, the corresponding microbial culture monitoring device comprises an incubator and an incubation rack, the incubation rack is arranged in the incubator, and the incubation rack is used to place a culture vessel containing a sample to be tested; the temperature control system comprises:

[0009] A temperature sensor provided corresponding to the incubation rack and used for measuring the temperature of the incubation rack to obtain a first temperature detection value of the incubation rack;

[0010] A heating part arranged corresponding to the incubation rack and used for heating the incubation rack;

[0011] A refrigeration component disposed on the incubator and used for cooling the incubator;

[0012] A controller is connected to the temperature sensor, the heating part and the refrigeration component respectively and is used to control the heating part and the refrigeration component according to the first temperature detection value obtained by the temperature sensor.

[0013] Optionally, the incubation rack is provided with a plurality of culture holes arranged in sequence, and the heating parts are respectively provided on at least two sides of the plurality of culture holes.

[0014] Optionally, the heating parts are respectively provided on two opposite sides of the incubation frame, and the culture holes are arranged in a straight line between the two opposite sides of the incubation frame.

[0015] Optionally, the temperature sensor is arranged at the center of the incubation rack.

[0016] Optionally, the refrigeration component is arranged at one end of the incubator, and the refrigeration component is located on one side of the incubation rack, and the temperature sensor corresponding to the incubation rack is arranged in the incubation rack and is located on the side of the culture hole close to the refrigeration component.

[0017] Optionally, the refrigeration component is arranged at one end of the incubator, and the refrigeration component includes a cold end located inside the incubator and a hot end located outside the incubator, the cold end is provided with a first fan for driving airflow to circulate between the incubation rack in the incubator and the refrigeration component, and the hot end is provided with a second fan for realizing outward heat dissipation of the refrigeration component.

[0018] Optionally, a plurality of incubation racks stacked in sequence are provided in the incubator, and the incubation racks are provided with a plurality of culture wells arranged in sequence, and the culture wells are used to place culture vessels containing samples to be tested.

[0019] Optionally, it also includes:

[0020] A temperature acquisition circuit corresponding to a single incubation rack and connected to the temperature sensor and the controller respectively, and used for acquiring an output signal of the temperature sensor;

[0021] A heating control circuit corresponding to the single incubation rack and connected to the heating part and the controller respectively, and used for controlling the heating part according to an output signal of the controller.

[0022] Optionally, it also includes: a refrigeration control circuit connected to the refrigeration component and the controller respectively, and used to control the refrigeration component according to an output signal of the controller.

[0023] Optionally, the heating portion includes a heating film.

[0024] It can be known from the above technical scheme that the temperature control system for microbial culture monitoring provided by the utility model, the corresponding microbial culture monitoring device includes an incubator and an incubation rack, the incubation rack is arranged in the incubator, and the incubation rack is used to place the culture vessel containing the sample to be tested. The temperature control system includes: a temperature sensor corresponding to the incubation rack, used to measure the temperature of the incubation rack to obtain a first temperature detection value of the incubation rack; a heating part corresponding to the incubation rack, used to heat the incubation rack; a refrigeration component arranged on the incubator, used to cool the incubator; a controller respectively connected to the temperature sensor, the heating part, and the refrigeration component, used to control the heating part and the refrigeration component according to the first temperature detection value obtained by the temperature sensor. The temperature control system for microbial culture monitoring of the utility model can heat the incubation rack with a single incubation rack as the control object, and can cool the incubator as a whole with the incubator as the control object, so as to realize the temperature control in the incubator used for microbial culture monitoring. Compared with the temperature control scheme of the prior art which adopts the overall heating and natural cooling of the incubator, the incubator used for microbial culture monitoring can adapt to a wider range of environmental temperatures, the target temperature range that can be set is wider, and the temperature uniformity in the incubator used for microbial culture monitoring can be improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0026] Figure 1 A longitudinal cross-sectional view of an incubator corresponding to a temperature control system for microbial culture monitoring provided by an embodiment of the utility model;

[0027] Figure 2 A front view of an incubator corresponding to a temperature control system for microbial culture monitoring provided by an embodiment of the utility model;

[0028] Figure 3 A schematic diagram of a temperature control system for microbial culture monitoring provided by an embodiment of the utility model;

[0029] Figure 4 A flow chart of a temperature control method applied to a temperature control system for microbial culture monitoring provided by an embodiment of the utility model;

[0030] Figure 5 A flow chart of a temperature control method applied to a temperature control system for microbial culture monitoring provided in yet another embodiment of the present invention.

[0031] The reference numerals in the drawings of the specification include:

[0032] 100 - incubator, 101 - incubation rack, 102 - culture well, 201 - temperature sensor, 202 - heating film, 203 - refrigeration component, 204 - first fan, 205 - second fan. DETAILED DESCRIPTION

[0033] In order to enable those skilled in the art to better understand the technical solutions in the present invention, the following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

[0034] This embodiment provides a temperature control system for microbial culture monitoring. The corresponding microbial culture monitoring device includes an incubator and an incubation rack. The incubation rack is arranged in the incubator and is used to place a culture vessel containing a sample to be tested. The temperature control system includes:

[0035] A temperature sensor provided corresponding to the incubation rack and used for measuring the temperature of the incubation rack to obtain a first temperature detection value of the incubation rack;

[0036] A heating part arranged corresponding to the incubation rack and used for heating the incubation rack;

[0037] A refrigeration component disposed on the incubator and used for cooling the incubator;

[0038] A controller is connected to the temperature sensor, the heating part and the refrigeration component respectively and is used to control the heating part and the refrigeration component according to the first temperature detection value obtained by the temperature sensor.

[0039] The incubation rack is arranged in an incubator, and the incubator provides the temperature required by the samples to be tested.

[0040] For any incubation rack, a temperature sensor and a heating unit are correspondingly provided, and the temperature sensor can detect and obtain a first temperature detection value of the incubation rack, and the first temperature detection value of the incubation rack reflects the temperature of the incubation rack, and the heating unit can heat the incubation rack. The incubator is cooled by the refrigeration component.

[0041] The temperature control system for microbial culture monitoring of the present embodiment can heat the incubation rack with a single incubation rack as the control object, and can cool the incubator as a whole with the incubator as the control object, so as to realize the temperature control in the incubator used for microbial culture monitoring. The prior art adopts the temperature control scheme of heating the incubator as a whole and naturally cooling it. Since it relies on natural cooling, it is not suitable for the situation where the target temperature is set lower than the ambient temperature. Compared with the prior art, the temperature control system for microbial culture monitoring of the present embodiment can heat the incubation rack with a single incubation rack as the control object, and cool the incubator as a whole with the incubator as the control object. The situation where the target temperature is set higher or lower than the ambient temperature can be applied. Therefore, the incubator used for microbial culture monitoring can adapt to a wider range of ambient temperatures, the target temperature range that can be set is wider, and the temperature uniformity in the incubator used for microbial culture monitoring can be improved.

[0042] In some embodiments, the incubation rack is provided with culture wells, and the culture vessels containing the samples to be tested are placed in the culture wells. In this embodiment, the location of the heating unit is not limited, as long as it is provided corresponding to the incubation rack and can heat the incubation rack. In some embodiments, the incubation rack is provided with a plurality of culture wells arranged in sequence, and the heating units are respectively provided on at least two sides of the plurality of culture wells, and each culture well is heated by the heating unit located on the side of the culture well. In this embodiment, the structure of the heating unit is not limited, and the heating unit can be, but is not limited to, a heating film.

[0043] In some embodiments, heating parts are respectively provided on two opposite sides of the incubation frame, and the culture wells are arranged in a straight line between the two opposite sides of the incubation frame, and the incubation frame is heated by the heating parts on both sides to achieve temperature control. Figure 1 , Figure 1 A longitudinal cross-sectional view of an incubator corresponding to a temperature control system for microbial culture monitoring provided in one embodiment is shown in the figure. As shown in the figure, an incubation rack 101 is provided in the incubator 100, and any incubation rack 101 is provided with two rows of culture holes 102, and each row of culture holes 102 is arranged in a "one" shape, and heating films 202 are respectively provided on the upper and lower parts of the incubation rack 101.

[0044] In some embodiments, a temperature sensor 201 may be provided at the center of the incubation rack 101. The temperature sensor 201 may be provided inside the housing of the incubation rack 101 at the center of the incubation rack 101, and the temperature of the incubation rack 101 may be detected by the temperature sensor 201. Figure 1 and Figure 2 , Figure 2 This is a front view of an incubator corresponding to a temperature control system for microbial culture monitoring provided in an embodiment. As shown in the figure, any incubation rack 101 is provided with two rows of culture wells 102, and a temperature sensor 201 is provided in the middle position between the two rows of culture wells 102. In this embodiment, the structure of the temperature sensor 201 is not limited, and a digital temperature sensor with higher reliability and anti-interference ability can be used, but is not limited to.

[0045] In some embodiments, the refrigeration component 203 is disposed at one end of the incubator 100, and the refrigeration component 203 is located on one side of the incubation rack 101. The heat in the incubator 100 is transferred to the outside of the incubator 100 through the refrigeration component 203, thereby achieving refrigeration and cooling of the incubator 100. The temperature sensor 201 corresponding to the incubation rack 101 can be disposed in the incubation rack 101 and on one side of the culture hole 102 close to the refrigeration component 203. For example, Figure 1As shown, any incubation rack 101 is provided with a temperature sensor 201 , and the temperature sensor 201 is arranged in the incubation rack 101 and is located on a side of the culture hole 102 close to the refrigeration component 203 .

[0046] In this embodiment, the structure of the refrigeration assembly 203 is not limited. In some embodiments, the refrigeration assembly 203 is arranged at one end of the incubator 100, and the refrigeration assembly 203 includes a cold end inside the incubator 100 and a hot end outside the incubator 100. Further, the cold end is provided with a first fan 204 for driving the airflow to circulate between the incubation rack 101 in the incubator 100 and the refrigeration assembly 203, and the hot end is provided with a second fan 205 for achieving heat dissipation of the refrigeration assembly 203. The first fan 204 drives the airflow to circulate between the incubation rack 101 and the refrigeration assembly 203 inside the incubator 100 to ensure the consistency of temperature during refrigeration. The second fan 205 efficiently dissipates the heat outside the incubator 100.

[0047] The refrigeration component 203 may include but is not limited to a semiconductor cooler (TEC), also known as a thermoelectric cooler, which is made using the Peltier effect of semiconductor materials. The Peltier effect, also known as the thermo-electric effect, refers to the phenomenon that when a direct current passes through a galvanic couple composed of two semiconductor materials, one end of the galvanic couple absorbs heat and the other end releases heat. The refrigeration capacity of a single thermoelectric refrigeration pair is limited, and it can be composed of a dozen to dozens of refrigeration pairs to form a TEC cooler or TEC refrigeration sheet. By controlling the direction of the current, the semiconductor cooler TEC can both cool and heat. The semiconductor cooler TEC has the characteristics of no noise, no vibration, no need for refrigerant, small size, light weight, etc., reliable operation, easy operation, fast cooling and heating, easy adjustment, and high temperature control accuracy. However, its refrigeration coefficient is relatively small, the refrigeration capacity is limited, the power consumption is relatively large, and the cost is relatively high. Therefore, it is mainly used in occasions with small cooling consumption and small floor space.

[0048] In this embodiment, there is no limitation on the number of incubation racks 101 included in the incubator 100, and there is no limitation on the number of culture holes 102 included in the incubation racks 101, and they can be flexibly set according to the needs in practical applications. In some embodiments, a plurality of incubation racks 101 stacked in sequence are provided in the incubator 100, and the incubation racks 101 are provided with a plurality of culture holes 102 arranged in sequence, and the culture holes 102 are used to place culture vessels containing samples to be tested. It can be that in the incubator 100, a plurality of incubation racks 101 stacked in sequence are provided between the front end and the rear end of the incubator 100, and the incubation racks 101 are provided with a plurality of culture holes 102 arranged in sequence, and the plurality of culture holes 102 are arranged in an array when observed from the front end to the rear end of the incubator 100, and the refrigeration assembly 203 is provided at the rear end of the incubator 100. For example, reference can be made to Figure 1 and Figure 2 Three horizontally placed incubation racks 101 are arranged inside the incubator 100. Each incubation rack 101 is provided with a plurality of culture holes 102 for placing culture bottles containing samples to be tested. As shown in the figure, each incubation rack 101 is provided with twenty culture holes 102.

[0049] In some embodiments, the temperature control system may further include: a temperature acquisition circuit corresponding to a single incubation rack 101 and connected to the temperature sensor 201 and the controller, respectively, for acquiring the output signal of the temperature sensor 201. A temperature sensor interface may also be included to provide a wiring harness interface for the temperature sensor 201. For example, reference may be made to Figure 3 , Figure 3 The schematic diagram of a temperature control system for monitoring microbial culture provided by an embodiment is shown in FIG. The temperature acquisition circuit is connected to the controller, and is connected to the temperature sensor interface through a wiring harness to connect to the temperature sensor 201 of the corresponding incubation rack. The temperature acquisition circuit is used to achieve signal isolation, level conversion or power supply of the temperature sensor 201.

[0050] In some embodiments, the temperature control system may further include: a heating control circuit corresponding to the single incubation rack 101 and connected to the heating unit and the controller respectively, for controlling the heating unit according to the output signal of the controller. It may also include a heating unit interface, which is a wiring harness interface of the heating unit. For example, reference may be made to Figure 3 The heating part adopts a heating film 202, the heating part interface is a heating film interface, the heating control circuit is connected to the controller, and is connected to the heating film interface through a wiring harness to be connected to the heating film 202 of the corresponding incubation rack.

[0051] In some embodiments, the temperature control system may further include: a refrigeration control circuit connected to the refrigeration component 203 and the controller respectively, and used to control the refrigeration component 203 according to the output signal of the controller. It may also include a refrigeration component interface, which is a wiring harness interface of the refrigeration component. For example, reference may be made to Figure 3 The refrigeration control circuit is connected to the controller, and is connected to the refrigeration component interface through a wiring harness to be connected to the refrigeration component 203.

[0052] The controller controls the temperature acquisition circuit to collect the output signals of the temperature sensors 201 of each incubation rack 101 in real time, and can perform PID operation on the collected temperature parameters. According to the operation result, the output power of the heating control circuit is changed to control the heating film 202 on each incubation rack 101 for temperature rise control of each incubation rack 101, or the output power of the refrigeration control circuit is changed to control the temperature drop of the entire incubator 100.

[0053] The temperature control system may also include a host computer, which is used to send the target temperature, incubation rack bias temperature and other PID control parameters that need to be set. The controller sets the output PWM duty cycle according to the PID operation result, and the heating control circuit controls the heating film 202 to heat according to the PWM duty cycle, and the cooling control circuit controls the cooling component 203 to cool according to the PWM duty cycle. The heating control circuit can be a heating PID control circuit, and the cooling control circuit can be a cooling PID control circuit. The controller can be a single-chip microcomputer.

[0054] The temperature control system for microbial culture monitoring of this embodiment, wherein the controller controls the heating part corresponding to the incubation rack and controls the refrigeration component according to the first temperature detection value obtained by the temperature sensor corresponding to the incubation rack, and the temperature control method applied can refer to Figure 4 , Figure 4 A flow chart of a temperature control method applied to a temperature control system for microbial culture monitoring provided in an embodiment is shown in the figure. The temperature control method includes the following steps:

[0055] S11: Acquire a first temperature detection value of the incubation rack and acquire a second temperature detection value of the incubator, wherein the second temperature detection value is a value obtained by performing a first preset statistical operation on the first temperature detection value of the incubation rack in the incubator.

[0056] The incubation rack is arranged in an incubator, and the incubator provides the temperature required by the samples to be tested.

[0057] For any incubation rack, the first temperature detection value of the incubation rack is obtained, and the first temperature detection value of the incubation rack reflects the temperature of the incubation rack. According to the first temperature detection value of the incubation rack in the incubator, the first temperature detection value is subjected to first preset statistics to obtain a second temperature detection value.

[0058] S12: taking a single incubation rack as a control object, and controlling heating of the incubation rack according to the first temperature detection value of the incubation rack, so that the first temperature detection value of the incubation rack approaches a first target temperature.

[0059] For any incubation rack, the heating of the incubation rack is controlled according to the first temperature detection value of the incubation rack, so that the first temperature detection value of the incubation rack approaches the first target temperature and the first temperature detection value of the incubation rack reaches the first target temperature.

[0060] S13: taking the incubator as a control object, and controlling refrigeration of the incubator according to the second temperature detection value of the incubator, so that the second temperature detection value of the incubator approaches a second target temperature.

[0061] For the incubator, the refrigeration of the incubator is controlled according to the second temperature detection value, so that the second temperature detection value of the incubator approaches the second target temperature, and the second temperature detection value of the incubator reaches the second target temperature.

[0062] In this embodiment, there is no limitation on the order of performing step S12 and step S13. Step S12 may be performed first and then step S13, or step S13 may be performed first and then step S12, or step S12 and step S13 may be performed simultaneously.

[0063] The temperature control system for microbial culture monitoring of this embodiment uses a single incubation rack as a control object to heat the incubation rack so that the temperature detection value of the incubation rack approaches the first target temperature, and uses the incubator as a control object to cool the incubator as a whole so that the temperature detection value of the incubator approaches the second target temperature. The prior art adopts a temperature control scheme of heating the incubator as a whole and cooling it naturally. Since it relies on natural cooling, it is not suitable for the case where the target temperature set is lower than the ambient temperature. Compared with the prior art, the temperature control method for microbial culture monitoring of this embodiment can use a single incubation rack as a control object to heat the incubation rack and use the incubator as a control object to cool the incubator as a whole. It can be applied to the case where the target temperature set is higher or lower than the ambient temperature. Therefore, the incubator used for microbial culture monitoring can adapt to a wider range of ambient temperatures, a wider range of target temperatures that can be set, and can improve the temperature uniformity in the incubator used for microbial culture monitoring.

[0064] In this embodiment, there is no limitation on the value of the first target temperature, and in practical applications, the first target temperature can be set according to the temperature requirements of the samples to be tested in the incubator.

[0065] The second temperature detection value is a value obtained by performing a first preset statistical analysis on the first temperature detection value of the incubation rack in the incubator. In some embodiments, a plurality of incubation racks are arranged in the incubator, and the second temperature detection value may be, but is not limited to, the maximum value, minimum value, average value or median value of the first temperature detection value of each incubation rack in the incubator.

[0066] In some embodiments, the second target temperature is a value obtained by performing a second preset statistical analysis on the first target temperature of the incubation rack of the incubator, so that heating control is performed on a single incubation rack and cooling control is performed on the incubator as a whole, but the heating control and cooling control use associated temperature parameters, so that the heating control and cooling control of the incubator are organically linked. In an embodiment where a plurality of incubation racks are provided in the incubator, the second target temperature may be, but is not limited to, the median, average, maximum or minimum value of the first target temperatures of the respective incubation racks of the incubator.

[0067] Exemplarily, multiple incubation racks are arranged in the incubator, the second temperature detection value is the maximum value of the first temperature detection values ​​of each incubation rack of the incubator, and the second target temperature is the middle value of the first target temperatures of each incubation rack of the incubator, which can be expressed as Tcs=MAX(Ts1, Ts2, Ts3, ..., Tsn), Tco=MID(To1, To2, To3, ..., Ton), wherein Tcs represents the second temperature detection value, Ts1, Ts2, Ts3, ..., Tsn represent the first temperature detection values ​​of each incubation rack respectively, Tco represents the second target temperature, To1, To2, To3, ..., Ton represent the first target temperature of each incubation rack respectively, n represents the number of incubation racks, MID() represents the function of calculating the middle value, and MAX() represents the function of calculating the maximum value.

[0068] In this embodiment, the maximum value of the first temperature detection value of each incubation rack of the incubator is used as the second temperature detection value, and the middle value of the first target temperature of each incubation rack of the incubator is used as the second target temperature, which has a better temperature control effect on the incubator. If PID control is used for heating control and PID control is used for cooling control, for heating PID control, the greater the difference between the temperature detection value and the target temperature, the greater the heating output power, and the greater the difference between the temperature detection value and the target temperature, the smaller the heating output power. ① If the maximum value of the first target temperature of each incubation rack is used as the second target temperature of the overall cooling, when approaching the second target temperature, except for the incubation racks whose first target temperature is the maximum value, the temperature detection value of other incubation racks is lower than the first target temperature of the incubation rack by a relatively large difference, thereby outputting a large heating power; excessive heating power will cause the temperature to overshoot in the opposite direction, breaking the original temperature balance, causing new temperature oscillations, and prolonging the temperature control process. ② If the minimum value of the first target temperature of each incubation rack is used as the second target temperature of the overall cooling, when approaching the second target temperature, the temperature detection value of the other incubation racks, except for the incubation racks whose first target temperature is the minimum value, is higher than the first target temperature of the incubation rack by a relatively large difference, thereby outputting very small heating power; too small heating power will also cause the temperature to overshoot in the opposite direction, breaking the original temperature balance, causing new temperature shocks, and prolonging the temperature control process. Therefore, in order to improve the overall temperature control efficiency, it is necessary to control the above two unfavorable factors ①② to a minimum. Taking the middle value of the first target temperature of the heating control of each incubation rack as the second target temperature for the overall cooling of the incubator will achieve the best effect.

[0069] In some embodiments, according to the first temperature detection value of the incubation rack, controlling the heating of the incubation rack so that the first temperature detection value of the incubation rack approaches a first target temperature comprises:

[0070] If the first temperature detection value of the incubation rack satisfies Ts<To-TL, the incubation rack is heated in a first heating manner so that the first temperature detection value of the incubation rack reaches To-TL≤Ts≤To+TH, wherein Ts represents the first temperature detection value of the incubation rack, To represents the first target temperature, To=Ti+Tj, Ti represents the original target temperature, Tj represents the bias temperature, and TL represents the first lower limit threshold;

[0071] If the first temperature detection value of the incubation rack satisfies To-TL≤Ts≤To+TH, the incubation rack is heated in a second heating manner so that the first temperature detection value of the incubation rack approaches the first target temperature, and TH represents a first upper limit threshold;

[0072] If the first temperature detection value of the incubation rack satisfies Ts>To+TH, the incubation rack is heated in a third heating manner so that the first temperature detection value of the incubation rack reaches To-TL≤Ts≤To+TH.

[0073] In this embodiment, there is no limitation on the values ​​of the original target temperature Ti, the bias temperature Tj, the first lower threshold TL, and the first upper threshold TH. In practical applications, they can be set according to the temperature requirements of the samples to be tested in the incubator, and can also be set and adjusted according to real-time conditions during the incubation monitoring process of the samples to be tested.

[0074] With a single incubation rack as the control object, the original target temperature Ti, the bias temperature Tj, the first lower threshold TL and the first upper threshold TH can be set for the single incubation rack. Through the above process, the heating control of the single incubation rack is independently performed to stabilize the temperature of the incubation rack at the first target temperature. The heating control mode for a single incubation rack can adopt a proportional-integral-derivative (PID) control method, and the proportional coefficient P, the integral coefficient I and the differential coefficient D can be set for a single incubation rack. In some embodiments, the first heating method includes controlling the heating of the incubation rack according to an adjustment amount of 100%; the second heating method includes controlling the heating of the incubation rack according to an adjustment amount obtained by performing a PID operation; the third heating method includes controlling the heating of the incubation rack according to an adjustment amount of 0.

[0075] Exemplarily, the process of the heating PID control mode is described in detail below, including the following steps:

[0076] Step 1, set the original target temperature Ti, bias temperature Tj, first lower threshold TL, first upper threshold TH, proportional coefficient P, integral coefficient I and differential coefficient D of the controlled object, the first target temperature of the controlled object is To=Ti+Tj, and the temperature detection value of the controlled object is represented by Ts. The bias temperature Tj is used to compensate for the actual temperature deviation caused by the local high or low temperature.

[0077] Step 2, when the heating PID control is in progress, when the temperature detection value of the controlled object Ts<To-TL, the adjustment amount output by the PID operation is 100%, and the incubation rack is heated at full power; when To-TL≤Ts≤To+TH, the heating of the incubation rack is controlled according to the adjustment amount obtained by the PID operation with the set duty cycle; the temperature of the controlled object will continue to rise due to inertia, resulting in overshoot; when Ts>To+TH, the adjustment amount obtained by the PID operation is 0, and Ts gradually decreases under the natural cooling effect caused by heat transfer and other non-control factors.

[0078] Step 3, when Ts decreases to To - TL ≤ Ts ≤ To + TH, heat the incubation rack according to the adjustment amount obtained from the PID operation, and adjust in such a cycle to stabilize the temperature of the controlled object at the first target temperature, that is, Ts = To. In the above process, the controlled object is a single incubation rack.

[0079] The proportional-integral-derivative (PID) control method maintains the state of the controlled object near the set value by continuously adjusting the output of the controller, and can achieve stable and accurate automatic control. In the temperature control method of this embodiment, a single incubation rack is used as the controlled object, and the PID control method is used to control the heating of the incubation rack, which can improve the control accuracy of temperature control and reduce the fluctuation.

[0080] In some embodiments, the first lower threshold TL is greater than zero, the first upper threshold TH is greater than zero, and the first lower threshold TL is greater than the first upper threshold TH. This can make To - TL lower than the original target temperature Ti, and To + TH slightly higher than the original target temperature Ti, that is, the heating PID control mode mainly works in the range below and slightly above the target temperature.

[0081] In some embodiments, according to the second temperature detection value of the incubator, controlling the refrigeration of the incubator to make the second temperature detection value of the incubator tend to the second target temperature includes:

[0082] If the second temperature detection value of the incubator satisfies Tcs > Tco + TcH, refrigerate the incubator in the first refrigeration mode to make the second temperature detection value of the incubator reach Tco - TcL ≤ Tcs ≤ Tco + TcH, where Tcs represents the second temperature detection value of the incubator, Tco represents the second target temperature, and TcH represents the second upper threshold;

[0083] If the second temperature detection value of the incubator satisfies Tco - TcL ≤ Tcs ≤ Tco + TcH, refrigerate the incubator in the second refrigeration mode to make the second temperature detection value of the incubator tend to the second target temperature, and TcL represents the second lower threshold;

[0084] If the second temperature detection value of the incubator satisfies Tcs < Tco - TcL, refrigerate the incubator in the third refrigeration mode to make the second temperature detection value of the incubator reach Tco - TcL ≤ Tcs ≤ Tco + TcH.

[0085] In this embodiment, the values of the second target temperature Tco, the second lower threshold TcL, and the second upper threshold TcH are not limited. In practical applications, they can be set according to the temperature requirements of the sample to be measured in the incubator, or can be set and adjusted according to the real-time situation during the cultivation and monitoring of the sample to be measured. Taking the incubator as the control object for refrigeration control, the second target temperature Tco, the second lower threshold TcL, and the second upper threshold TcH can be set for the incubator. Through the above process, the refrigeration control of the incubator is performed to make the temperature of the incubator stable at the second target temperature.

[0086] The refrigeration control mode of the incubator can adopt the Proportional-Integral-Derivative (PID) control method. The proportional coefficient P, the integral coefficient I, and the differential coefficient D can be set for the incubator. In some embodiments, the first refrigeration method includes controlling the refrigeration of the incubator according to an adjustment amount of 100%; the second refrigeration method includes controlling the refrigeration of the incubator according to the adjustment amount obtained by PID operation; the third refrigeration method includes controlling the refrigeration of the incubator according to an adjustment amount of 0.

[0087] Exemplarily, the process of the refrigeration PID control mode is described in detail below, including the following steps:

[0088] Step 1: First, set the second lower threshold TcL, the second upper threshold TcH, the proportional coefficient P, the integral coefficient I, and the differential coefficient D of the control object; then, calculate the intermediate value of the first target temperature of each incubation rack of the incubator as the second target temperature of the control object, denoted as Tco = MID(To1, To2, To3,..., Ton), and calculate the maximum value of the temperature detection values of each incubation rack of the incubator as the reference temperature of the control object, denoted as Tcs = MAX(Ts1, Ts2, Ts3,..., Tsn).

[0089] Step 2: When the refrigeration PID control is in progress, when the reference temperature value of the control object satisfies Tcs > Tco + TcH, the adjustment amount output by the PID operation is 100%, and the incubator is refrigerated at full power; when Tco - TcL ≤ Tcs ≤ Tco + TcH, the incubator is refrigerated according to the adjustment amount obtained by the PID operation at the set duty cycle; due to inertia, the temperature of the control object will continue to decrease and overshoot will occur; when Tcs < Tco - TcL, the adjustment amount obtained by the PID operation is 0; under the action of natural heating caused by heat transfer and other non-control factors, Tcs gradually increases.

[0090] Step 3, when Tcs rises to Tco - TcL ≤ Tcs ≤ Tco + TcH, refrigeration is carried out according to the adjustment amount obtained by PID operation, and such adjustment is cycled to achieve the temperature of the controlled object to be stabilized at the second target temperature, that is, Tcs = Tco. In the above process, the controlled object is the entire incubator.

[0091] In the temperature control method of this embodiment, the incubator is used as the controlled object, and the incubator is cooled as a whole. The PID control method is used to control the refrigeration of the incubator, which can improve the control accuracy of temperature control and reduce the fluctuation.

[0092] In some embodiments, the second lower threshold TcL is greater than zero, the second upper threshold TcH is greater than zero, and the second lower threshold TcL is less than the second upper threshold TcH, which can make Tco - TcL of the refrigeration control close to the second target temperature Tco, and Tco + TcH higher than the second target temperature Tco, that is, the refrigeration PID control mode mainly works in the interval close to and higher than the second target temperature.

[0093] In some embodiments, the first lower threshold TL is greater than zero, the first upper threshold TH is greater than zero, and the first lower threshold TL is greater than the first upper threshold TH, the second lower threshold TcL is greater than zero, the second upper threshold TcH is greater than zero, and the second lower threshold TcL is less than the second upper threshold TcH. If To = Tco, then TL > TcL, TH < TcH, that is to say, it can make the heating PID control mode mainly work in the interval lower than the original target temperature and slightly higher than the original target temperature, and the refrigeration PID control mode mainly work in the interval close to and higher than the second target temperature. Exemplarily, the set original target temperature Ti = 30 °C; TL = 0.5 °C, TH = 0.1 °C for heating control, the adjustment interval of heating PID control: 29.5 - 30.1 °C; TcL = 0.1 °C, TcH = 0.5 °C for refrigeration control; the adjustment interval of refrigeration PID control: 29.9 - 30.5 °C.

[0094] When the set original target temperature is close to the ambient temperature, the natural cooling and natural heating capabilities caused by heat transfer and other non - control factors are both weak and unstable. Using only the refrigeration control mode or the heating control mode alone cannot achieve temperature control. In this case, the heating control of a single incubation rack and the refrigeration control of the incubator are turned on simultaneously. By using this special control method, through the first temperature detection value obtained from the incubation rack and the associated temperature parameters, the two are organically linked together, which can not only run simultaneously but also switch in real - time to jointly achieve temperature control. The operating principle is as follows:

[0095] ① The cooling PID control and the heating PID control run at the same time, but the PID parameters and PID operations of the two are completely isolated and independent of each other. The cooling PID control performs overall cooling control on the entire incubator according to the first temperature detection value of each incubation rack, and the heating PID control performs local temperature increase control on a single incubation rack according to the first temperature detection value of the corresponding incubation rack, forming a control strategy that combines overall cooling control with local temperature increase control.

[0096] ② When the refrigeration PID control and the heating PID control are running at the same time, the control parameters of the heating PID control (P, I, D, TL, TH and other parameters) can be modified to weaken the heating and temperature rise regulation function of the heating PID control, forming a regulation mechanism with refrigeration PID control as the main and heating PID control as the auxiliary. That is, a control strategy with refrigeration PID control as the main and heating PID control as the auxiliary is adopted.

[0097] ③ When the cooling PID control and the heating PID control are running simultaneously, according to the changing trend of the reference temperature (Ts or Tcs), by changing the exit conditions of the two PID controls (parameters such as Tk, TL, TH or Tck, TcL, TcH), the system can be stabilized to a control mode as soon as possible.

[0098] In some embodiments, if the first temperature detection value of the incubation rack satisfies Ts>To+TH+Tk, the heating control of the incubation rack is turned off, wherein Ts represents the first temperature detection value of the incubation rack, To represents the first target temperature, TH represents the first upper limit threshold, and Tk represents the third threshold. When the set original target temperature Ti is lower than the ambient temperature, the natural heating ability caused by heat transfer and other non-control factors is stronger, and the natural cooling ability is weaker. When Ts>To+TH, the heating of the incubation rack is controlled with an adjustment amount of 0; Ts cannot be gradually reduced, but gradually increased; this change trend is detected, and when Ts reaches Ts>To+TH+Tk, that is, Ts>Ti+Tj+Tk+TH, the heating PID control of the incubation rack is turned off. After the heating control of the incubation rack is turned off, the refrigeration control runs independently, and the entire incubator forms an independent temperature control unit. Under the refrigeration PID control, refrigeration and cooling are adjusted according to the change of the second temperature detection value until the second target temperature is reached. In some embodiments, the fan at the cold end of the refrigeration assembly can be turned on to circulate the air in the incubator between the cold end of the refrigeration assembly and the incubation rack to improve the temperature consistency in the incubator. In this state, the factors affecting the temperature are mainly the cooling capacity determined by the output power of the refrigeration assembly under PID control and the heating capacity caused by natural cold loss.

[0099] Tk represents the difference between the upper limit temperature of heating control shutdown and the upper limit temperature To+TH of heating control, that is, the temperature rise threshold at which the adjustment amount of heating control output is 0. When the natural temperature rise is strong, when Ts reaches the upper limit temperature To+TH of heating control, the adjustment amount of heating control output is 0. After the Ts rise exceeds the overshoot range, Ts will continue to rise. When Ts>To+TH+Tk, the heating control is turned off and the cooling control is turned on. Tk must be greater than the heating overshoot.

[0100] In some embodiments, the second target temperature is a value obtained by performing a second preset statistical analysis on the first target temperature of the incubation rack of the incubator; if the second temperature detection value of the incubator satisfies Tcs<Tco-TcL-Tck, the refrigeration control of the incubator is turned off, wherein Tcs represents the second temperature detection value of the incubator, Tco represents the second target temperature, TcL represents the second lower limit threshold, and Tck represents the fourth threshold. When the set original target temperature Ti is higher than the ambient temperature, the natural cooling ability caused by heat transfer and other non-control factors is stronger, and the natural heating ability is weaker. In refrigeration control, if Tcs<Tco-TcL, the adjustment amount of the PID operation output is 0, and the temperature of Tcs cannot be gradually increased, but gradually decreased; when this change trend is detected, when Tcs reaches the set condition, that is, Tcs<Tco-TcL-Tck, the refrigeration PID control is turned off. The heating PID control of each incubation rack operates independently to form an independent temperature control unit; the incubation rack performs heating and temperature adjustment according to the change of the first temperature detection value under the corresponding heating PID control until the first target temperature is reached, achieving the purpose of precise temperature control. At this time, the factors affecting temperature regulation are mainly the heating capacity determined by the output power of the heating part under the heating PID control and the cooling capacity caused by natural heat loss.

[0101] Tck represents the difference between the lower limit temperature of cooling control shutdown and the lower limit temperature of cooling control Tco-TcL, that is, the temperature drop threshold value at which the adjustment amount of cooling control output is 0. When the natural cooling is strong, when Tcs reaches the lower limit temperature Tco-TcL of cooling control, the adjustment amount of cooling control output is 0. After the Tcs drop reaches the maximum overshoot, Tcs will continue to drop. Tcs<Tco-TcL-Tck, the cooling control is turned off and the heating control is turned on. Tck should be greater than the cooling overshoot.

[0102] The original target temperature Ti is set slightly higher than the ambient temperature, and the natural cooling is slightly stronger than the natural heating. When the cooling PID control and the heating PID control are running at the same time, the detected temperature will continue to be low. When this trend is detected, the exit condition of the cooling PID control mode will be modified to exit the mode as soon as possible, and only the heating PID control mode will be retained. The conditions for modifying parameters such as Tck, TcL, and TcH include: 1. The number of consecutive occurrences of Tcs<Tco exceeds the set threshold; it means that the temperature of the controlled object continues to be lower than the target temperature; 2. The integral calculation result of the cooling PID is negative and less than the set threshold; the temperature continues to be lower than the target temperature, and the error is negative (cooling error Tcs-Tco, heating error To-Ts, the temperature control direction is opposite), and the cumulative error integral calculation result will become a negative value with a large absolute value, indicating that the error is continuing to increase, and the cooling PID adjustment can no longer play a role in temperature control. At the same time, the above two conditions are met, and the exit condition is modified to close the cooling PID control mode as soon as possible. It can also be closed directly, but it will cause temperature fluctuations, and the effect is not as good as modifying the conditions to gradually close.

[0103] The original target temperature Ti is set slightly lower than the ambient temperature, and the natural heating is slightly stronger than the natural cooling. When the cooling PID control mode and the heating PID control mode are running at the same time, the detected temperature will continue to be high, and the exit condition of the heating PID control mode will be modified to make it exit as soon as possible, and only the cooling PID control mode will be retained. The conditions for modifying parameters such as Tk, TL, and TH include: 1. The number of consecutive occurrences of Ts>To exceeds the set threshold; it means that the temperature of the controlled object continues to be higher than the target temperature; 2. The integral calculation result of the heating PID is negative and less than the set threshold; the temperature continues to be higher than the target temperature, and the error is negative (cooling error Tcs-Tco, heating error To-Ts, temperature control direction is opposite), and the cumulative error integral calculation result will become a negative value with a large absolute value, indicating that the error is continuing to increase, and the heating PID adjustment cannot play a role in temperature control. At the same time, the above two conditions are met, and the exit condition is modified to close the heating PID control mode as soon as possible. It can also be closed directly, but it will cause temperature fluctuations, and the effect is not as good as modifying the conditions to gradually close.

[0104] In some embodiments, before a single incubation rack is controlled as a control object and the heating of the incubation rack is controlled according to the first temperature detection value of the incubation rack, it may also include: if the first temperature detection value of the incubation rack satisfies Ts<To-TL-Tk, the heating control of the incubation rack is started, that is, the incubation rack is controlled as a control object and the heating of the incubation rack is controlled according to the first temperature detection value of the incubation rack so that the first temperature detection value of the incubation rack approaches a first target temperature, Ts represents the first temperature detection value of the incubation rack, To represents the first target temperature, TL represents a first lower threshold value, and Tk represents a third threshold value. Figure 5, Figure 5 A flow chart of a temperature control method applied to a temperature control system for microbial culture monitoring provided in another embodiment is shown in the figure. A first temperature detection value of an incubation rack and a second temperature detection value of an incubator are obtained. If the first temperature detection value of the incubation rack satisfies Ts<To-TL-Tk, heating control of the incubation rack is started first; if the second temperature detection value satisfies Tcs>Tco+TcH+Tck, cooling control of the incubator is started. Otherwise, cooling control of the incubator is not started. Whether cooling of the incubator needs to be started also depends on the natural heating and cooling capacity.

[0105] In some embodiments, before controlling the refrigeration of the incubator according to the second temperature detection value of the incubator with the incubator as the control object, the method further includes: if the second temperature detection value satisfies Tcs>Tco+TcH+Tck, then starting the refrigeration control of the incubator, that is, controlling the refrigeration of the incubator according to the second temperature detection value of the incubator with the incubator as the control object, so that the second temperature detection value of the incubator approaches the second target temperature, Tcs represents the second temperature detection value of the incubator, Tco represents the second target temperature, TcH represents the second upper limit threshold, and Tck represents the fourth threshold. Figure 5 As shown, the first temperature detection value of the incubation rack and the second temperature detection value of the incubator are obtained. If the second temperature detection value satisfies Tcs>Tco+TcH+Tck, the refrigeration control of the incubator is started first; if the first temperature detection value of the incubation rack satisfies Ts<To-TL-Tk, the heating control of the incubation rack is started, otherwise the heating control of the incubation rack is not started. Whether the heating of the incubation rack needs to be started also depends on the natural heating and cooling capacity.

[0106] The utility model scheme aims at the defects of the existing technical scheme and has the following advantages:

[0107] a) Use heating film with a cost far lower than TEC to heat each incubation rack module, achieve temperature regulation, and save instrument manufacturing costs; each incubation rack module has its own temperature measurement sensor, adopts independent PID control, and can set the target temperature and bias temperature separately. The bias temperature is used to compensate for the local high or low temperature, solve the problem of inconsistent temperature of each incubation rack module during heating, and improve the temperature uniformity of the incubator;

[0108] b) Using TEC refrigeration components to cool the incubator as a whole reduces the number of TECs used, reduces instrument power consumption, and saves instrument manufacturing costs; adding a fan to the refrigerator component strengthens the air circulation inside the incubator and improves the uniformity of the temperature inside the box during cooling; the TEC refrigeration component only performs cooling and cooling control and is not used for heating, which can save the control circuit that changes the TEC current direction, making the instrument design simpler and saving instrument costs; improving heating efficiency and reducing instrument power consumption.

[0109] c) The temperature acquisition solution uses a digital temperature sensor with stronger reliability and anti-interference ability, which reduces the complexity of system design, improves system reliability and fault tolerance, and saves instrument costs.

[0110] d) The middle value of the target temperature of each incubation rack is used as the target temperature of the refrigeration PID control of the TEC refrigeration component, and the maximum value of the temperature measurement value of each incubation rack module measurement sensor is used as the reference temperature of the refrigeration PID control of the TEC refrigeration component. The advantage of this is that it saves the number of temperature measurement sensors used and reduces the manufacturing cost of the instrument; the refrigeration PID control mode and the heating PID control mode can work better together by using the same temperature parameters.

[0111] e) The cooling PID control mode of the TEC cooling component and the heating PID control mode of each incubation rack module are both independent and interconnected, and complement each other. They can run simultaneously and switch in real time to achieve temperature control together. Through the control strategy of combining overall cooling control with local heating control, using the cooling PID control mode as the main and the heating PID control as the auxiliary, and modifying the exit conditions of the control mode in real time, not only the problems of control accuracy, volatility, uniformity, etc. are solved, but also the stability of the system is improved.

[0112] The above is a detailed introduction to a temperature control system for microbial culture monitoring provided by the utility model. This article uses specific examples to illustrate the principle and implementation method of the utility model. The description of the above embodiment is only used to help understand the method and core idea of ​​the utility model. It should be pointed out that for ordinary technicians in this technical field, without departing from the principle of the utility model, the utility model can also be improved and modified, and these improvements and modifications also fall within the scope of protection of the claims of the utility model.

Claims

1. A temperature control system for microbial culture monitoring, characterized in that: The corresponding microbial culture monitoring device includes an incubator and an incubation rack, wherein the incubation rack is arranged in the incubator and is used to place a culture vessel containing a sample to be tested; the temperature control system includes: A temperature sensor provided corresponding to the incubation rack and used for measuring the temperature of the incubation rack to obtain a first temperature detection value of the incubation rack; A heating part arranged corresponding to the incubation rack and used for heating the incubation rack; A refrigeration component disposed on the incubator and used for cooling the incubator; A controller is connected to the temperature sensor, the heating part and the refrigeration component respectively and is used to control the heating part and the refrigeration component according to the first temperature detection value obtained by the temperature sensor.

2. The temperature control system for microbial culture monitoring according to claim 1, characterized in that: The incubation rack is provided with a plurality of culture holes arranged in sequence, and the heating parts are respectively provided on at least two sides of the plurality of culture holes.

3. The temperature control system for microbial culture monitoring according to claim 2, characterized in that: The heating parts are respectively arranged on two opposite sides of the incubation frame, and the culture holes are arranged in a straight line between the two opposite sides of the incubation frame.

4. The temperature control system for microbial culture monitoring according to claim 1, characterized in that: The temperature sensor is arranged at the center of the incubation rack.

5. The temperature control system for microbial culture monitoring according to claim 1, characterized in that: The refrigeration component is arranged at one end of the incubator and is located at one side of the incubation rack. The temperature sensor corresponding to the incubation rack is arranged in the incubation rack and is located at a side of the culture hole close to the refrigeration component.

6. The temperature control system for microbial culture monitoring according to claim 1, characterized in that: The refrigeration component is arranged at one end of the incubator, and the refrigeration component includes a cold end inside the incubator and a hot end outside the incubator. The cold end is provided with a first fan for driving airflow to circulate between the incubation rack in the incubator and the refrigeration component, and the hot end is provided with a second fan for achieving outward heat dissipation of the refrigeration component.

7. The temperature control system for microbial culture monitoring according to claim 1, characterized in that: A plurality of incubation racks stacked in sequence are arranged in the incubator, and the incubation racks are provided with a plurality of culture holes arranged in sequence, and the culture holes are used to place culture vessels containing samples to be tested.

8. The temperature control system for microbial culture monitoring according to claim 1, characterized in that: Also includes: A temperature acquisition circuit corresponding to a single incubation rack and connected to the temperature sensor and the controller respectively, and used for acquiring an output signal of the temperature sensor; A heating control circuit corresponding to the single incubation rack and connected to the heating part and the controller respectively, and used for controlling the heating part according to an output signal of the controller.

9. The temperature control system for microbial culture monitoring according to claim 1, characterized in that: Also includes: A refrigeration control circuit connected to the refrigeration component and the controller respectively and used for controlling the refrigeration component according to an output signal of the controller.

10. The temperature control system for microbial culture monitoring according to claim 1, characterized in that: The heating portion includes a heating film.

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