Adsorption device for plasmid extraction and suction filtration

Abstract

The utility model relates to the technical field of plasmid extraction, in particular to an adsorption device for plasmid extraction and suction filtration, which comprises a centrifugal tube, a partition plate and a round cover, the partition plate is used for dividing the inside of the centrifugal tube into a first chamber and a second chamber, the round cover is rotatably connected to an opening of the centrifugal tube, a through hole is arranged on the round cover, and the axial direction of the through hole is parallel to the axial direction of the round cover. The through hole deviates from the center of the round cover and is used for containing an adsorption column pipe, the diameter of the adsorption column pipe is smaller than that of the first limiting opening, the diameter of the adsorption column pipe is smaller than that of the second limiting opening, a first limiting plate and a second limiting plate are arranged below the round cover, the structure of the first limiting plate is the same as that of the second limiting plate, and the first limiting plate is matched with the first limiting opening. By means of the structure, the adsorption column tube can be switched back and forth between the first cavity and the second cavity, the situation that the adsorption column tube is taken by hands or experiment tools is avoided, and therefore pollution to the adsorption column tube is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of plasmid extraction, in particular to an adsorption device used for plasmid extraction and filtration. Background Art

[0002] The commonly used method for large-scale plasmid extraction is to use a silicon matrix membrane adsorption column to bind plasmid DNA based on the alkaline lysis method to lyse cells, then transfer the adsorption column to a new collection tube, add an eluent and centrifuge, so that the plasmid in the adsorption column and the eluent enter the new collection tube together. However, during the process of transferring the adsorption column to the new collection tube, the adsorption column is easily contaminated by taking it by hand or experimental tools, resulting in contamination of the plasmid DNA in the adsorption column, thereby reducing the purity of the plasmid extraction. Therefore, there is an urgent need for an adsorption device for plasmid extraction and filtration, which can reduce the risk of contamination of the adsorption column during the transfer process, thereby improving the purity of the plasmid extraction. Utility Model Content

[0003] The technical problem to be solved by the utility model is to provide an adsorption device for plasmid extraction and filtration, which can reduce the risk of contamination of the adsorption column during the transfer process, thereby improving the purity of the plasmid extraction.

[0004] In order to solve the above technical problems, the technical solution adopted by the utility model is: an adsorption device for plasmid extraction and filtration, comprising:

[0005] A centrifuge tube, wherein an opening is provided at the top of the centrifuge tube;

[0006] A partition, the partition is connected to the centrifuge tube, the partition is used to divide the centrifuge tube into a first chamber and a second chamber, the partition includes a first flat plate and a second flat plate, the first flat plate coincides with the centrifuge tube axially, the upper edge of the first flat plate is flush with the opening, the upper end of the second flat plate is connected to the lower end of the first flat plate, the lower end of the first flat plate is connected to the inner wall of the centrifuge tube, the first flat plate is provided with a first limiting opening and a second limiting opening, the first limiting opening and the second limiting opening are symmetrically arranged with respect to the axis of the centrifuge tube;

[0007] A round cover, the round cover can be rotatably connected to the opening, a through hole is provided on the round cover, the axial direction of the through hole is parallel to the axial direction of the round cover, the through hole deviates from the center of the round cover, the through hole is used to place an adsorption column tube, the diameter of the adsorption column tube is smaller than the first limiting opening, the diameter of the adsorption column tube is smaller than the second limiting opening, a first limiting plate and a second limiting plate are provided below the round cover, the structure of the first limiting plate is the same as the second limiting structure, the first limiting plate matches the first limiting opening, and the second limiting plate matches the second limiting opening;

[0008] The round cover rotates at the opening to switch the round cover between a first state and a second state;

[0009] In the first state, the adsorption column tube is located in the first chamber, the first limiting plate is embedded in the first limiting opening, and the second limiting plate is embedded in the second limiting opening;

[0010] In the second state, the adsorption column tube is located in the second chamber, the first limiting plate is embedded in the second limiting opening, and the second limiting plate is embedded in the first limiting opening.

[0011] Furthermore, a limiting hole is provided in the middle of the first flat plate, the opening of the limiting hole is oriented in the same direction as the opening of the centrifuge tube, the axial direction of the limiting hole is coaxial with the centrifuge tube, a limiting column is provided below the round cover to match the gap with the limiting hole, the limiting column is coaxial with the centrifuge tube, and a handle is provided on the upper part of the round cover.

[0012] Furthermore, a first sealing ring is arranged in the first limiting opening, and a second sealing ring is arranged in the second limiting opening.

[0013] Furthermore, the adsorption column tube is provided with a detachable tube cover.

[0014] Furthermore, a drain port is provided at the bottom end of the second flat plate relative to the inner wall of the centrifuge tube, a drain valve is provided on the drain port, and the drain valve is connected to the drain port by interference fit.

[0015] The beneficial effect of the utility model is that, compared with the prior art, since the adsorption column tube rotates from the first chamber to the second chamber in the centrifuge tube, there is no need to use experimental tools or hold the adsorption column tube by hand, thereby avoiding the adsorption column tube from being contaminated by hands or experimental tools, and improving the purity of plasmid extraction. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the structure of an adsorption device for plasmid extraction and filtration according to a specific embodiment of the utility model;

[0017] Figure 2 A top view of an adsorption device for plasmid extraction and filtration according to a specific embodiment of the utility model;

[0018] Figure 3 for Figure 2 A-direction cross-sectional view;

[0019] Figure 4 A side view of an adsorption device for plasmid extraction and filtration according to a specific embodiment of the utility model;

[0020] Figure 5 for Figure 4 B-direction cross-sectional view;

[0021] Figure 6 for Figure 5 Part A is the big picture.

[0022] Description of symbols

[0023] 1. Centrifuge tube; 11. Drain port;

[0024] 2. partition; 21. first flat plate; 211. first limiting opening; 2111. first sealing ring; 212. second limiting opening; 2121. second sealing ring; 213. limiting hole; 22. second flat plate;

[0025] 3. round cover; 31. first limiting plate; 32. second limiting plate; 33. through hole; 34. limiting column; 35. handle;

[0026] 4. Adsorption column;

[0027] 5. Tube cover;

[0028] 6. Drain valve. DETAILED DESCRIPTION

[0029] In order to explain the technical content, achieved objectives and effects of the present invention in detail, the following is an explanation in conjunction with the implementation modes and the accompanying drawings.

[0030] Please refer to Figures 1 to 6 , 1. An adsorption device for plasmid extraction and filtration, comprising:

[0031] A centrifuge tube 1, wherein an opening is provided at the top of the centrifuge tube 1;

[0032] A partition 2 is connected to the centrifuge tube 1. The partition 2 is used to divide the centrifuge tube 1 into a first chamber and a second chamber. The partition 2 includes a first flat plate 21 and a second flat plate 22. The first flat plate 21 coincides with the centrifuge tube 1 axially. The upper edge of the first flat plate 21 is flush with the opening. The upper end of the second flat plate 22 is connected to the lower end of the first flat plate 21. The lower end of the first flat plate 21 is connected to the inner wall of the centrifuge tube 1. The first flat plate 21 is provided with a first limiting opening 211 and a second limiting opening 212. The first limiting opening 211 and the second limiting opening 212 are symmetrically arranged with respect to the axis of the centrifuge tube 1.

[0033] The circular cover 3 is rotatably connected to the opening. A through hole 33 is provided on the circular cover 3. The axial direction of the through hole 33 is parallel to the axial direction of the circular cover 3. The through hole 33 deviates from the center of the circular cover 3. The through hole 33 is used to place the adsorption column tube 4. The diameter of the adsorption column tube 4 is smaller than the first limiting opening 211. The diameter of the adsorption column tube 4 is smaller than the second limiting opening 212. A first limiting plate 31 and a second limiting plate 32 are provided below the circular cover 3. The structure of the first limiting plate 31 is the same as that of the second limiting structure. The first limiting plate 31 matches the first limiting opening 211, and the second limiting plate 32 matches the second limiting opening 212.

[0034] The round cover 3 rotates at the opening, so that the round cover 3 switches between the first state and the second state;

[0035] In the first state, the adsorption column tube 4 is located in the first chamber, the first limiting plate 31 is embedded in the first limiting opening 211, and the second limiting plate 32 is embedded in the second limiting opening 212;

[0036] In the second state, the adsorption column tube 4 is located in the second chamber, the first limiting plate 31 is embedded in the second limiting opening 212 , and the second limiting plate 32 is embedded in the first limiting opening 211 .

[0037] In the above implementation mode, in the first state, the supernatant of the lysate after multiple centrifugation of the bacteria is poured into the adsorption column tube 4, and then the centrifuge tube 1 is placed in the centrifuge for centrifugation. After the centrifugation is completed, the plasmid is combined with the adsorption column and adsorbed, and the waste liquid enters the first chamber. Then, by rotating the round cover 3, the adsorption column is transferred from the first state to the second state, and then the eluent is poured into the adsorption column tube 4, and the centrifuge tube 1 is placed in the centrifuge for centrifugation. After the centrifugation is completed, the eluent elutes the plasmid adsorbed by the adsorption column from the adsorption column, and the plasmid and the eluent are gathered at the bottom of the second chamber. Compared with the prior art, since the adsorption column tube 4 is rotated from the first chamber to the second chamber in the centrifuge tube 1, there is no need to use experimental tools or hand-holding the adsorption column tube 4, thereby avoiding the adsorption column tube 4 from being contaminated by hand or experimental tools, and the purity of the plasmid extraction is improved.

[0038] As an optional embodiment, a limiting hole 213 is provided in the middle of the first flat plate 21, the opening of the limiting hole 213 is oriented in the same direction as the opening of the centrifuge tube 1, the axial direction of the limiting hole 213 is coaxial with the centrifuge tube 1, a limiting column 34 is provided below the round cover 3 and is clearance-matched with the limiting hole 213, the limiting column 34 is coaxial with the centrifuge tube 1, and a handle 35 is provided on the upper part of the round cover 3.

[0039] In the above embodiment, the limiting column 34 of the round cover 3 is placed in the limiting hole 213, so that the lower end of the limiting column 34 contacts the inner wall of the bottom end of the limiting hole 213, so that the round cover 3 is located above the opening, and then the handle 35 is grasped by hand to rotate the round cover 3 to make the limiting column 34 rotate in the limiting hole 213, so that the adsorption column tube 4 is transferred from the first chamber to the second chamber through the first limiting port 211, the first limiting plate 31 is transferred from the first limiting port 211 to the second limiting port 212, and the second limiting plate 32 is transferred from the second limiting port 212 to the first limiting port 211, thereby ensuring that the adsorption column tube 4 rotates in the centrifuge tube 1, thereby reducing the contamination of the adsorption column.

[0040] As an optional implementation, a first sealing ring 2111 is disposed in the first limiting opening 211 , and a second sealing ring 2121 is disposed in the second limiting opening 212 .

[0041] In the above embodiment, the sealing of the first chamber and the second chamber is ensured when the round cover 3 is in the first state and the second state, and liquid leakage is prevented from occurring in the first chamber and the second chamber.

[0042] As an optional implementation, the adsorption column tube is provided with a detachable tube cover 5 .

[0043] In the above embodiment, an internal thread is provided at the opening of the adsorption column tube 4, and an external thread is provided on the tube cover 5. The internal thread is connected to the external thread, so that the tube cover 5 can be detachably connected to the adsorption column tube 4, and the tube cover 5 and the adsorption column tube 4 are sealed, ensuring that the adsorption column tube 4 is sealed during the transfer of the round cover 3, thereby preventing pollutants from falling from the opening of the adsorption column tube 4 into the adsorption column tube 4, thereby contaminating the adsorption column.

[0044] As an optional embodiment, a drain port 11 is provided at the bottom end of the second flat plate 22 relative to the inner wall of the centrifuge tube 1 , a drain valve 6 is provided on the drain port 11 , and the drain valve 6 is connected to the drain port 11 by interference fit.

[0045] In the above embodiment, after the drain valve 6 is separated from the drain port 11 , the waste liquid in the first chamber is discharged from the drain port 11 .

[0046] The above description is only an embodiment of the present invention, and does not limit the patent scope of the present invention. Any equivalent transformations made using the contents of the specification and drawings of the present invention, or directly or indirectly applied in the relevant technical field, are also included in the patent protection scope of the present invention.

Claims

1. An adsorption device for plasmid extraction and filtration, characterized in that: include: A centrifuge tube, wherein an opening is provided at the top of the centrifuge tube; A partition, the partition is connected to the centrifuge tube, the partition is used to divide the centrifuge tube into a first chamber and a second chamber, the partition includes a first flat plate and a second flat plate, the first flat plate coincides with the centrifuge tube axially, the upper edge of the first flat plate is flush with the opening, the upper end of the second flat plate is connected to the lower end of the first flat plate, the lower end of the first flat plate is connected to the inner wall of the centrifuge tube, the first flat plate is provided with a first limiting opening and a second limiting opening, the first limiting opening and the second limiting opening are symmetrically arranged with respect to the axis of the centrifuge tube; A round cover, the round cover can be rotatably connected to the opening, a through hole is provided on the round cover, the axial direction of the through hole is parallel to the axial direction of the round cover, the through hole deviates from the center of the round cover, the through hole is used to place an adsorption column tube, the diameter of the adsorption column tube is smaller than the first limiting opening, the diameter of the adsorption column tube is smaller than the second limiting opening, a first limiting plate and a second limiting plate are provided below the round cover, the structure of the first limiting plate is the same as the second limiting structure, the first limiting plate matches the first limiting opening, and the second limiting plate matches the second limiting opening; The round cover rotates at the opening to switch the round cover between a first state and a second state; In the first state, the adsorption column tube is located in the first chamber, the first limiting plate is embedded in the first limiting opening, and the second limiting plate is embedded in the second limiting opening; In the second state, the adsorption column tube is located in the second chamber, the first limiting plate is embedded in the second limiting opening, and the second limiting plate is embedded in the first limiting opening.

2. The adsorption device for plasmid extraction and filtration according to claim 1, characterized in that: A limiting hole is provided in the middle of the first flat plate, the opening of the limiting hole is oriented in the same direction as the opening of the centrifuge tube, the axial direction of the limiting hole is coaxial with the centrifuge tube, a limiting column is provided below the round cover to match the clearance of the limiting hole, the limiting column is coaxial with the centrifuge tube, and a handle is provided on the top of the round cover.

3. The adsorption device for plasmid extraction and filtration according to claim 1, characterized in that: A first sealing ring is arranged in the first limiting opening, and a second sealing ring is arranged in the second limiting opening.

4. The adsorption device for plasmid extraction and filtration according to claim 1, characterized in that: The adsorption column tube is equipped with a removable tube cover.

5. The adsorption device for plasmid extraction and filtration according to claim 1, characterized in that: A liquid discharge port is arranged at the bottom end of the second flat plate relative to the inner wall of the centrifuge tube, a liquid discharge valve is arranged on the liquid discharge port, and the liquid discharge valve is connected to the liquid discharge port by interference fit.

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