Efficient separation device for preparing exosome

Abstract

The utility model discloses an efficient separation device for preparing exosomes. The efficient separation device comprises a cylinder body, a driving gear, a driven gear, a centrifugal cylinder and a cover plate, a plurality of centrifugal cylinders are arranged in the cylinder body; rotating shafts are mounted at the bottoms of the centrifugal cylinders; the rotating shaft penetrates through the bottom of the barrel and is rotationally and hermetically connected with the bottom of the barrel; a driving gear is arranged at the center of the lower part of the barrel body; a gear shaft at one end of the driving gear is rotationally connected with the barrel, and the other end is driven by a driving motor; driven gears as many as the centrifugal cylinders are arranged on the periphery of the driving gear; the driven gear is meshed with the driving gear; a gear shaft of the driven gear is connected with the rotating shaft through a coupler; a cover plate is arranged above the cylinder body; a sample injection cavity is formed in the cover plate; sleeves of which the number is equal to that of the centrifugal cylinders are arranged below the sample injection cavity; a sample injection hole communicated with the sample injection cavity is formed in the sleeve; a sample inlet communicated with the sample inlet cavity is formed in the top of the cover plate. According to the utility model, the separation efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of exosome preparation, and in particular to a high-efficiency separation device for preparing exosomes. Background Art

[0002] Exosomes refer to small membrane vesicles containing complex RNA and proteins. Nowadays, they specifically refer to disc-shaped vesicles with a diameter of 40-100nm. A variety of cells can secrete exosomes under normal and pathological conditions. They are mainly derived from multivesicular bodies formed by the invagination of intracellular lysosomal particles. After the multivesicular outer membrane fuses with the cell membrane, they are released into the extracellular matrix. In order to improve the efficiency of research on exosomes, it is necessary to separate exosomes. For this purpose, an efficient separation device for exosomes is proposed. Utility Model Content

[0003] The technical problem to be solved by the utility model is to provide a high-efficiency separation device for preparing exosomes to improve the separation efficiency.

[0004] In order to solve the above technical problems, the utility model adopts the following technical solutions: an efficient separation device for preparing exosomes, comprising a cylinder, a driving gear, a driven gear, a centrifugal cylinder and a cover plate;

[0005] A plurality of centrifugal cylinders are arranged in the cylinder; a rotating shaft is installed at the bottom of the centrifugal cylinder; the rotating shaft passes through the bottom of the cylinder, and the rotating shaft is rotatably and sealedly connected to the bottom of the cylinder; a driving gear is arranged at the center of the lower part of the cylinder; a gear shaft at one end of the driving gear is rotatably connected to the cylinder, and the other end is driven by a driving motor; a driven gear equal in number to the number of centrifugal cylinders is arranged on the outer periphery of the driving gear; the driven gear meshes with the driving gear; the gear shaft of the driven gear is connected to the rotating shaft through a coupling; a cover plate is arranged above the cylinder; a sample injection cavity is arranged in the cover plate; a sleeve equal in number to the number of centrifugal cylinders is arranged below the sample injection cavity; a sample injection hole connected to the sample injection cavity is arranged in the sleeve; a sample injection port connected to the sample injection cavity is arranged on the top of the cover plate.

[0006] Furthermore, the sample inlet is connected to the liquid storage tank through a pipeline and a pump body.

[0007] Furthermore, there are multiple centrifuge barrel fixing frames in the barrel body; a rotating shaft is arranged at the bottom of the centrifuge barrel fixing frame; the centrifuge barrel fixing frame includes a bottom support plate; a support ring is arranged above the bottom support plate; the support ring is connected to the support plate through a connecting rod; multiple limit slots are arranged in the support ring; and a limit protrusion matching the limit slot is arranged on the outside of the centrifuge barrel.

[0008] Furthermore, a movable ring rotatably connected to the cover plate is arranged on the outer periphery of the sleeve; and a ball bearing is arranged on the surface of the movable ring in contact with the cover plate.

[0009] Furthermore, the centrifugal cylinder includes an upper cylinder and a lower cylinder; the upper cylinder and the lower cylinder are screwed together; a filter body is arranged in the upper cylinder; the lower cylinder includes a hollow inner cylinder and a hollow outer cylinder; a filter layer is arranged between the inner cylinder and the outer cylinder.

[0010] Furthermore, the cover plate is connected to the cylinder body through a connecting piece; the connecting piece includes an inverted T-shaped slot arranged on the top of the cylinder body, L-shaped block 1 and block 2 and a bidirectional lead screw arranged on the cover plate; the block 1 and block 2 are slidingly connected to the cover plate, and the block 1 and block 2 are threadedly connected to the bidirectional lead screw, and both ends of the bidirectional lead screw are rotatably connected to the bearing seat on the cover plate; one end of the bidirectional lead screw is fixedly connected to the knob.

[0011] The beneficial effects of the utility model are as follows: in this embodiment, a plurality of separation cylinders are driven to work by an active gear, and the solid matter in the stock liquid is screened out by setting the separation cylinder structure, so as to avoid the solid matter in the stock liquid from causing the device to be blocked in the subsequent separation process, thereby improving the separation efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] The utility model is further described below in conjunction with the accompanying drawings and embodiments.

[0013] Figure 1 It is a structural schematic diagram of the utility model.

[0014] Figure 2 A top view of the interior of the centrifuge cylinder.

[0015] Figure 3 Schematic diagram of the activity ring.

[0016] Figure 4 Schematic diagram of the centrifuge cylinder structure.

[0017] Figure 5 This is a schematic diagram of the structure of Example 2.

[0018] Figure 6 Schematic diagram of the centrifuge tube fixing frame.

[0019] Figure 7 It is a schematic diagram of the connection between the centrifuge cylinder fixing frame and the separation cylinder.

[0020] Figure 8 Schematic diagram of the connection between the cover plate and the cylinder. DETAILED DESCRIPTION

[0021] The technical solution of the utility model will be clearly and completely described below through specific implementation methods. Example 1

[0022] refer to Figure 1-Figure 2, is a high-efficiency separation device for preparing exosomes of the utility model, comprising a cylinder 1, a driving gear 2, a driven gear 3, a centrifugal cylinder 4 and a cover plate 5.

[0023] In this embodiment, a plurality of centrifugal cylinders 4 are arranged in the cylinder 1; a rotating shaft 8 is installed at the bottom of the centrifugal cylinder 4; the rotating shaft 8 passes through the bottom of the cylinder 1, and the rotating shaft 8 is rotatably and sealedly connected to the bottom of the cylinder 1; a driving gear 2 is arranged at the center below the cylinder 1; a gear shaft at one end of the driving gear 2 is rotatably connected to the cylinder 1, and the other end is driven by a driving motor; a driven gear 3 equal in number to the number of centrifugal cylinders 4 is arranged on the periphery of the driving gear 2; the driven gear 3 is meshed with the driving gear 2; the gear shaft of the driven gear 3 is connected to the rotating shaft 8 through a coupling; a cover plate 5 is arranged above the cylinder 1; a sample injection cavity 51 is arranged in the cover plate 5; a sleeve 52 equal in number to the number of centrifugal cylinders 4 is arranged below the sample injection cavity 51; a sample injection hole 53 connected to the sample injection cavity 51 is arranged in the sleeve 52; a sample injection port 54 connected to the sample injection cavity 51 is arranged on the top of the cover plate 5.

[0024] Preferably, Figure 3 As shown, a movable ring 55 rotatably connected to the cover plate 5 is arranged on the outer periphery of the sleeve 52 ; and balls are arranged on the surface of the movable ring 55 in contact with the cover plate 5 .

[0025] By setting the movable ring, the top of the centrifugal cylinder 4 contacts the movable ring, and the friction between the top of the cylinder and the cover plate 5 is reduced during the rotation of the centrifugal cylinder, thereby avoiding affecting the rotation speed of the centrifugal cylinder.

[0026] like Figure 4 As shown, the centrifugal cylinder 4 of this embodiment includes an upper cylinder 41 and a lower cylinder 42; a filter body 43 is arranged in the upper cylinder 41; the filter body 43 includes a carbon nanotube composite graphene filter layer, the carbon nanotube composite graphene filter layer is fixed on the filter frame, and the filter frame is installed in the upper cylinder 41.

[0027] The lower cylinder 42 of this embodiment includes a hollow inner cylinder 421 and a hollow outer cylinder 422; a filter layer 423 is arranged between the inner cylinder and the outer cylinder.

[0028] The upper cylinder 41 and the inner cylinder are connected by threads, the bottom of the inner cylinder is sealed, a number of circular holes are arranged on the side wall of the inner cylinder, a filter layer is wrapped around the outside of the inner cylinder, the filter layer is a sleeve-shaped carbon nanotube composite graphene filter layer, which is sleeved on the outside of the inner cylinder, the outer cylinder is sleeved on the outside of the carbon nanotube composite graphene filter layer, the outer cylinder and the inner cylinder are fixed by screws, and the outer cylinder is covered with circular holes.

[0029] The pore size of the carbon nanotube composite graphene filter layer in the filter body 43 is larger than the pore size of the carbon nanotube composite graphene filter layer in the filter layer 423 .

[0030] In this embodiment, the driving gear drives several separation cylinders to work. Through the setting of the separation cylinder structure, the solid matter in the raw liquid is screened out, and the solid matter is retained in the upper cylinder 41 above the filter body 43. Through the centrifugal operation, the exosomes are retained in the lower cylinder 42, thereby improving the separation efficiency. A liquid outlet may be provided at the bottom of the cylinder 1. Example 2

[0031] like Figure 5 As shown, the sample inlet 54 of this embodiment is connected to the liquid storage tank through a pipeline and a pump body, so that manual sample injection is not required, thereby improving separation efficiency. Example 3

[0032] like Figure 6 and 7 As shown, in the present embodiment, there are multiple centrifugal barrel fixing frames 9 in the barrel body 1; a rotating shaft 8 is arranged at the bottom of the centrifugal barrel fixing frame 9; the centrifugal barrel fixing frame includes a bottom support plate 91; a support ring 92 is arranged above the bottom support plate 91; the support ring 92 is connected to the support plate 91 through a connecting rod 93; a plurality of limit slots 94 are arranged in the support ring 92; and a limit protrusion 95 matching the limit slot 94 is arranged on the outer side of the centrifugal barrel 4.

[0033] In this embodiment, a centrifuge cylinder fixing frame is provided to facilitate installation and removal of the centrifuge cylinder. Example 4

[0034] In this embodiment, the cover plate 5 is connected to the cylinder body 1 via a connecting piece, which may be a connecting bolt.

[0035] like Figure 8 As shown, the connecting member in this embodiment includes an inverted T-shaped groove 101 arranged on the top of the cylinder 1, an L-shaped block 102 and a block 2 103 and a bidirectional lead screw 104 arranged on the cover plate 5; the block 102 and the block 2 103 are slidingly connected to the cover plate 5, and the block 102 and the block 2 103 are screwed to the bidirectional lead screw 104, and the two ends of the bidirectional lead screw are rotatably connected to the bearing seat on the cover plate 5; one end of the bidirectional lead screw is fixedly connected to the knob 105.

[0036] This embodiment facilitates the installation of the cover plate.

[0037] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Those skilled in the art may make various modifications or equivalent substitutions to the present invention within the essence and protection scope of the present invention, and such modifications or equivalent substitutions should also be deemed to fall within the protection scope of the technical solution of the present invention.

Claims

1. An efficient separation device for preparing exosomes, characterized in that: It comprises a cylinder (1), a driving gear (2), a driven gear (3), a centrifugal cylinder (4) and a cover plate (5); A plurality of centrifugal cylinders (4) are arranged in the cylinder (1); a rotating shaft (8) is installed at the bottom of the centrifugal cylinder (4); the rotating shaft (8) passes through the bottom of the cylinder (1), and the rotating shaft (8) is rotatably and hermetically connected to the bottom of the cylinder (1); a driving gear (2) is arranged at the center below the cylinder (1); a gear shaft at one end of the driving gear (2) is rotatably connected to the cylinder (1), and the other end is driven by a driving motor; the outer periphery of the driving gear (2) is provided with driven gears (3) equal in number to the number of centrifugal cylinders (4). ; the driven gear (3) is meshed with the driving gear (2); the gear shaft of the driven gear (3) is connected to the rotating shaft (8) via a coupling; a cover plate (5) is arranged above the cylinder (1); a sample injection cavity (51) is arranged inside the cover plate (5); sleeves (52) equal in number to the number of centrifugal cylinders (4) are arranged below the sample injection cavity (51); a sample injection hole (53) communicating with the sample injection cavity (51) is arranged inside the sleeve (52); and a sample injection port (54) communicating with the sample injection cavity (51) is arranged on the top of the cover plate (5).

2. The high-efficiency separation device for preparing exosomes according to claim 1, characterized in that: The sample inlet (54) is connected to the liquid storage tank (7) via a pipeline and a pump body (6).

3. The efficient separation device for preparing exosomes according to claim 1, characterized in that: The barrel (1) includes a plurality of centrifugal barrel fixing frames (9); a rotating shaft (8) is arranged at the bottom of the centrifugal barrel fixing frame (9); the centrifugal barrel fixing frame comprises a bottom support plate (91); a support ring (92) is arranged above the bottom support plate (91); the support ring (92) is connected to the support plate (91) via a connecting rod (93); a plurality of limiting grooves (94) are arranged in the support ring (92); and limiting protrusions (95) matching the limiting grooves (94) are arranged on the outer side of the centrifugal barrel (4).

4. The efficient separation device for preparing exosomes according to claim 1, characterized in that: The outer periphery of the sleeve (52) is provided with a movable ring (55) rotatably connected to the cover plate (5); a ball bearing is provided on the surface of the movable ring (55) in contact with the cover plate (5).

5. The efficient separation device for preparing exosomes according to claim 1, characterized in that: The centrifugal cylinder (4) comprises an upper cylinder (41) and a lower cylinder (42); the upper cylinder (41) and the lower cylinder (42) are screwed together; a filter body is arranged inside the upper cylinder (41); the lower cylinder (42) comprises a hollow inner cylinder and a hollow outer cylinder; a filter layer is arranged between the inner cylinder and the outer cylinder.

6. The efficient separation device for preparing exosomes according to claim 1, characterized in that: The cover plate (5) is connected to the cylinder (1) via a connecting piece; the connecting piece comprises an inverted T-shaped slot (101) arranged at the top of the cylinder (1), an L-shaped block 1 (102) and block 2 (103) arranged on the cover plate (5), and a bidirectional lead screw (104); the block 1 (102) and block 2 (103) are slidably connected to the cover plate (5), and the block 1 (102) and block 2 (103) are screwed to the bidirectional lead screw (104), and both ends of the bidirectional lead screw are rotatably connected to the bearing seat on the cover plate (5); one end of the bidirectional lead screw is fixedly connected to the knob (105).

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