Stem cell exosome separation and extraction device

Abstract

The utility model relates to the field of stem cell treatment, in particular to a stem cell exosome separation and extraction device which comprises a machine base used for protection, a sealing cover, a centrifugal mechanism used for providing centrifugal power and a clamping mechanism used for clamping a test tube. An oscillation mechanism for promoting oscillation of a test tube is installed at the axis position of the centrifugal mechanism, and the multiple sets of clamping mechanisms are evenly distributed on the centrifugal mechanism along the circumference. Through cooperation of the oscillation mechanism, the centrifugal mechanism and the clamping mechanism, the test tube is driven to turn over by utilizing centrifugal force, meanwhile, the vibration provided by the oscillation mechanism for the fixing frame is utilized to generate mixing of various powers when stem cell exosomes in the test tube are centrifuged, separation of the stem cell exosomes is improved, and therefore the separation effect is improved.

Description

Technical Field

[0001] The utility model relates to the field of stem cell processing, in particular to a stem cell exosome separation and extraction device. Background Art

[0002] The stem cell exosome separation and extraction device is a device used to separate and extract stem cell exosomes from cell culture supernatant or biological samples. Exosomes are small vesicles secreted by cells that contain a variety of bioactive molecules, such as miRNA, proteins and other bioactive molecules, and have important biological functions and potential clinical application value. The device usually uses centrifugation, ultrafiltration, or affinity adsorption techniques to separate and extract exosomes from samples. Such devices are usually precisely designed to efficiently extract exosomes and maintain their integrity and bioactivity to meet scientific research and clinical needs.

[0003] In the prior art, a Chinese utility model with a comparative announcement number of CN220610828U discloses a stem cell exosome separation and extraction device, which uses a movable block to facilitate the placement of test tubes. However, in actual centrifugal operations, because the liquid needs to be separated from the exosomes, a single centrifugal force cannot quickly and effectively separate the solid and liquid, and it is often necessary to increase the separation time to achieve the desired effect. Utility Model Content

[0004] The purpose of the utility model is to provide a stem cell exosome separation and extraction device in order to solve the above problems.

[0005] The utility model achieves the above-mentioned purpose through the following technical solutions:

[0006] A stem cell exosome separation and extraction device, comprising a machine base and a sealing cover for protection, a centrifugal mechanism for providing centrifugal power, and a clamping mechanism for clamping a test tube, wherein the centrifugal mechanism is installed inside the machine base, an oscillation mechanism for causing the test tube to oscillate is installed at the axis position of the centrifugal mechanism, and the clamping mechanism is provided in a plurality of groups, and the plurality of groups of the clamping mechanisms are evenly distributed on the centrifugal mechanism along the circumference;

[0007] The centrifugal mechanism comprises a rotating base, a rotating column is installed at the axis position of the rotating base, a rotating table is installed at the upper end of the rotating column, and a matching groove for matching the oscillation mechanism is provided on the rotating table;

[0008] The clamping mechanism includes a fixed frame, and a first power rod and a second power rod are respectively arranged at both ends of the fixed frame, the first power rod and the second power rod are rotatably connected to the fixed frame, the first power rod and the second power rod are located on one end of the fixed frame and a power gear ring is installed, a through hole for placing a test tube is formed on the fixed frame, a passive ring is arranged on the inner wall of the through hole, an active ring is arranged on the lower side of the passive ring, and the active ring cooperates with the two power gear rings through a gear block.

[0009] Preferably: the oscillation mechanism includes a telescopic sleeve, which is installed along the radial direction of the rotating table and the telescopic sleeve is installed corresponding to the position of the fixed frame, an oscillation generating column is installed inside the matching groove, a telescopic rod is installed on the telescopic sleeve corresponding to the position of the oscillation generating column, an extrusion spring is installed between the telescopic sleeve and the telescopic rod, and a protrusion is formed on the oscillation generating column corresponding to the position of the telescopic sleeve.

[0010] Preferably, the second power rod and the first power rod are both connected to the power gear ring via ratchets, and the ratchets of the second power rod and the first power rod are in opposite directions.

[0011] Preferably, the passive ring and the active ring are rotatably connected to the fixing frame, and rubber pads are installed on the inner sides of the passive ring and the active ring.

[0012] Preferably, the first power rod and the second power rod are connected to the rotating table via splines at one end away from the fixing frame.

[0013] Preferably, the top of the oscillation generating column is connected to the sealing cover via threads, and the sealing cover is connected to the base via snaps.

[0014] Preferably, the telescopic sleeve is slidably connected to the rotating platform, and a ball head is formed on one end of the telescopic sleeve located on the fixing frame.

[0015] Compared with the prior art, the beneficial effects are as follows:

[0016] Through the cooperation of the oscillation mechanism, the centrifugal mechanism and the clamping mechanism, the centrifugal force is used to drive the test tube to flip. At the same time, the vibration provided by the oscillation mechanism to the fixed frame generates a mixture of multiple forces when the stem cell exosomes inside the test tube are centrifuged, thereby improving the separation of the stem cell exosomes and thus improving the separation effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0018] Figure 1 This is a schematic diagram of the structure of a stem cell exosome separation and extraction device described in the utility model;

[0019] Figure 2 This is a schematic diagram of the installation structure of an oscillation generating column of a stem cell exosome separation and extraction device described in the utility model;

[0020] Figure 3 This is a schematic diagram of the centrifugal mechanism structure of a stem cell exosome separation and extraction device described in the utility model;

[0021] Figure 4 This is a schematic diagram of the structure of a fixing frame of a stem cell exosome separation and extraction device described in the utility model;

[0022] Figure 5 This is a schematic diagram of the internal structure of a fixing frame of a stem cell exosome separation and extraction device described in the utility model;

[0023] Figure 6 This is a schematic diagram comparing the structures of a fixing frame of a stem cell exosome separation and extraction device described in the utility model before and after flipping;

[0024] Figure 7 This is a top view of a rotating table of a stem cell exosome separation and extraction device described in the utility model;

[0025] Figure 8 It is a schematic diagram of the internal structure of a telescopic sleeve of a stem cell exosome separation and extraction device described in the utility model.

[0026] The following are the descriptions of the reference numerals:

[0027] 1. Machine base; 2. Sealing cover; 3. Oscillation mechanism; 4. Centrifugal mechanism; 5. Clamping mechanism; 31. Oscillation generating column; 32. Telescopic sleeve; 33. Telescopic rod; 34. Extrusion spring; 41. Rotating base; 42. Rotating column; 43. Matching groove; 44. Rotating table; 51. Fixed frame; 52. First power rod; 53. Passive ring; 54. Active ring; 55. Second power rod; 56. Power gear ring. DETAILED DESCRIPTION

[0028] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood by specific circumstances.

[0029] The utility model is further described below in conjunction with the accompanying drawings:

[0030] like Figure 1-Figure 8 As shown, a stem cell exosome separation and extraction device includes a base 1 for protection, a sealing cover 2, a centrifugal mechanism 4 for providing centrifugal power, and a clamping mechanism 5 for clamping a test tube. The centrifugal mechanism 4 is installed inside the base 1, and an oscillation mechanism 3 for causing the test tube to oscillate is installed at the axis position of the centrifugal mechanism 4. The clamping mechanism 5 is provided with a plurality of groups, and the plurality of groups of clamping mechanisms 5 are evenly distributed on the centrifugal mechanism 4 along the circumference;

[0031] The centrifugal mechanism 4 includes a rotating base 41, a rotating column 42 is installed at the axis position of the rotating base 41, a rotating table 44 is installed on the upper end of the rotating column 42, a matching groove 43 for matching the oscillation mechanism 3 is provided on the rotating table 44, and a power motor is installed at the bottom of the rotating base 41;

[0032] like Figure 3-5 As shown, the clamping mechanism 5 includes a fixed frame 51, and the first power rod 52 and the second power rod 55 are respectively arranged at both ends of the fixed frame 51. The first power rod 52 and the second power rod 55 are rotatably connected to the fixed frame 51. The first power rod 52 and the second power rod 55 are located at one end of the fixed frame 51 and a power gear ring 56 is installed. A through hole for placing a test tube is formed on the fixed frame 51, and a passive ring 53 is arranged on the inner wall of the through hole. An active ring 54 is arranged on the lower side of the passive ring 53, and the active ring 54 cooperates with the two power gear rings 56 through a tooth block.

[0033] Preferred: Figure 8 As shown, the oscillation mechanism 3 includes a telescopic sleeve 32, which is installed along the radial direction of the rotating table 44 and the telescopic sleeve 32 is installed at a position corresponding to the fixed frame 51. An oscillation generating column 31 is installed inside the matching groove 43, and a telescopic rod 33 is installed at a position of the telescopic sleeve 32 corresponding to the oscillation generating column 31. An extrusion spring 34 is installed between the telescopic sleeve 32 and the telescopic rod 33, and a protrusion is formed on the oscillation generating column 31 at a position corresponding to the telescopic sleeve 32.

[0034] Preferred: Figure 5As shown, the second power rod 55 and the first power rod 52 are both connected to the power gear ring 56 through a ratchet, and the ratchet directions of the second power rod 55 and the first power rod 52 are opposite, so that when the fixed frame 51 is flipped back and forth, the positive and negative power gear ring 56 can cooperate with the active ring 54 to rotate the test tube.

[0035] Preferably, the passive ring 53 and the active ring 54 are rotatably connected to the fixing frame 51, and rubber pads are installed on the inner sides of the passive ring 53 and the active ring 54, so as to ensure the fixation of the test tube.

[0036] Preferably, the first power rod 52 and the second power rod 55 are both connected to the rotating platform 44 via splines at one end away from the fixing frame 51 .

[0037] Preferably, the top of the oscillation generating column 31 is connected to the sealing cover 2 via threads, and the sealing cover 2 is connected to the base 1 via snaps.

[0038] Preferably, the telescopic sleeve 32 is slidably connected to the rotating platform 44 , and one end of the telescopic sleeve 32 located on the fixing frame 51 is formed into a ball head.

[0039] Working principle: Place multiple test tubes in the through holes of the fixing frame 51, and use the protrusions on the top of the test tubes to prevent them from falling. At this time, use the rubber pads of the passive ring 53 and the active ring 54 to fix the test tubes, and close the machine base 1;

[0040] The power motor at the bottom of the rotating base 41 is started, and the rotating base 41, the rotating column 42, the rotating table 44, the six fixed racks 51 and the test tubes fixed thereon start to rotate. Since the test tubes contain stem cells, the test tubes and the fixed racks 51 begin to flip over due to the centrifugal force. Figure 6 As shown, after the flipping occurs, the fixing frame 51 squeezes the telescopic sleeve 32;

[0041] When the telescopic sleeve 32 feels squeezed, the telescopic sleeve 32 squeezes the internal squeezing spring 34, so that the squeezing spring 34 is slowly tightened. At the same time, because the other end of the squeezing spring 34 corresponds to the telescopic rod 33, the telescopic rod 33 is intermittently matched with the protrusion on the oscillation generating column 31, and the oscillation generating column 31 can reversely provide oscillation force to the telescopic sleeve 32 and the fixing frame 51, thereby providing oscillation for the test tube and the stem cells inside.

[0042] At the same time, because the test tube and the fixed frame 51 oscillate, when the first power rod 52 and the second power rod 55 cooperate with the reciprocating fixed frame 51, the power gear ring 56 connected by the ratchet on the first power rod 52 and the second power rod 55 can intermittently cooperate with the power gear ring 56, and the power gear ring 56 drives the test tube to rotate, thereby improving the stirring effect of the stem cells inside the test tube, and utilizing the combination of centrifugal force and multiple external forces to improve the effect of stem cell separation.

[0043] The above shows and describes the basic principle, main features and advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments, and the above embodiments and descriptions are only for explaining the principle of the utility model. Without departing from the spirit and scope of the utility model, the utility model may have various changes and improvements, and these changes and improvements fall within the scope of the utility model to be protected.

Claims

1. A stem cell exosome separation and extraction device, comprising a base (1) for protection and a sealing cover (2), characterized in that: It also comprises a centrifugal mechanism (4) for providing centrifugal power and a clamping mechanism (5) for clamping the test tube, the centrifugal mechanism (4) being installed inside the machine base (1), an oscillation mechanism (3) for causing the test tube to oscillate being installed at the axis center position of the centrifugal mechanism (4), and the clamping mechanism (5) being provided in a plurality of groups, and the plurality of groups of the clamping mechanisms (5) being evenly distributed on the centrifugal mechanism (4) along the circumference; The centrifugal mechanism (4) comprises a rotating base (41), a rotating column (42) is installed at the axis position of the rotating base (41), a rotating table (44) is installed at the upper end of the rotating column (42), and a matching groove (43) for matching the oscillating mechanism (3) is provided on the rotating table (44); The clamping mechanism (5) comprises a fixed frame (51), and a first power rod (52) and a second power rod (55) are respectively arranged at two ends of the fixed frame (51), and the first power rod (52) and the second power rod (55) are rotatably connected to the fixed frame (51), and a power gear ring (56) is installed on one end of the first power rod (52) and the second power rod (55) located on the fixed frame (51). A through hole for placing a test tube is formed on the fixed frame (51), and a passive ring (53) is arranged on the inner wall of the through hole. An active ring (54) is arranged on the lower side of the passive ring (53), and the active ring (54) cooperates with two power gear rings (56) through a tooth block.

2. A stem cell exosome separation and extraction device according to claim 1, characterized in that: The oscillation mechanism (3) comprises a telescopic sleeve (32), the telescopic sleeve (32) being installed along the radial direction of the rotating platform (44), and the telescopic sleeve (32) being installed at a position corresponding to the fixed frame (51), an oscillation generating column (31) being installed inside the matching groove (43), a telescopic rod (33) being installed at a position of the telescopic sleeve (32) corresponding to the oscillation generating column (31), a compression spring (34) being installed between the telescopic sleeve (32) and the telescopic rod (33), and a protrusion being formed on the oscillation generating column (31) at a position corresponding to the telescopic sleeve (32).

3. A stem cell exosome separation and extraction device according to claim 1, characterized in that: The second power rod (55) and the first power rod (52) are both connected to the power gear ring (56) via ratchets, and the ratchets of the second power rod (55) and the first power rod (52) have opposite directions.

4. A stem cell exosome separation and extraction device according to claim 3, characterized in that: The passive ring (53) and the active ring (54) are rotatably connected to the fixing frame (51), and rubber pads are installed on the inner sides of the passive ring (53) and the active ring (54).

5. The stem cell exosome separation and extraction device according to claim 3, characterized in that: The ends of the first power rod (52) and the second power rod (55) away from the fixed frame (51) are both connected to the rotating platform (44) via splines.

6. A stem cell exosome separation and extraction device according to claim 2, characterized in that: The top of the oscillation generating column (31) is connected to the sealing cover (2) via a thread, and the sealing cover (2) is connected to the machine base (1) via a buckle.

7. The stem cell exosome separation and extraction device according to claim 2, characterized in that: The telescopic sleeve (32) is slidably connected to the rotating platform (44), and one end of the telescopic sleeve (32) located on the fixing frame (51) is formed into a ball head.

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