Antigenic cell collecting device

Abstract

The utility model discloses an antigen cell collecting device which comprises a collecting cylinder, the top of the collecting cylinder is in threaded connection with a sealing cover, the top of the sealing cover is provided with a liquid inlet, the inner wall of the collecting cylinder is rotatably connected with a centrifugal cylinder, the bottom of the centrifugal cylinder is fixedly connected with an outer gear ring, and the outer gear ring is fixedly connected with an inner gear ring. A centrifugal motor is installed on the right side of the collecting cylinder, a gear is fixedly connected to an output shaft of the centrifugal motor, a through groove is formed in the right side of the collecting cylinder, the gear penetrates through the through groove and is meshed with the outer gear ring, and a guide funnel is fixedly connected to the inner wall of the collecting cylinder. According to the utility model, the sealed centrifugal cylinder is arranged to carry out centrifugal separation on a dendritic cell solution added with a medium, so that the separation effect is good, cells needing to be collected are easier to obtain, and the height of the solution in the centrifugal cylinder can be known through the cooperation of the liquid level sensor and the display controller; the cells in the interface layer can be more accurately collected through the collection assembly, and the collection precision is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of cell collection, in particular to an antigen cell collection device. Background Art

[0002] Antigen cells usually refer to cells that can produce antigens. Antigens refer to molecules that can be recognized by the immune system and can trigger an immune response. They can be proteins, polysaccharides, lipids or nucleic acids.

[0003] Such as dendritic cells and macrophages, they can take up antigens, process them and present them to T cells, thereby initiating adaptive immune responses.

[0004] The patent document with application number CN202122704272.8 discloses a dendritic cell collection device, including: a box body, a baffle, a first feed port, a second feed port, a filter screen, a separation box, a first connecting pipe, a motor, a first bevel gear, a rotating shaft, a second bevel gear, a paddle, a collection box and a second connecting pipe; the box body is a hollow cavity, and a first mounting plate and a second mounting plate are provided in the box body, and there is a distance between the first mounting plate and the second mounting plate, which can divide the box body into a first accommodating chamber, a second accommodating chamber and a third accommodating chamber; the baffle is vertically fixed to the bottom surface of the first accommodating chamber, and the first accommodating chamber is divided into a filtering chamber and a fixed chamber; the first feed port is arranged on the top surface of the box body, and the first feed port is connected to the filtering chamber; the second feed port is arranged on the top surface of the box body, the second feed port is opposite to the first feed port, and the second feed port is connected to the filtering chamber.

[0005] In this structure, multiple cavities are set up to allow the cell solution to flow in each cavity in sequence, realizing the steps of filtration, centrifugation and collection. However, the centrifugal effect in this structure is poor. The cell solution cannot be stratified by a single set of stirring shafts. At the same time, the solution needs to continue to flow after centrifugation, resulting in mixing that affects the collection accuracy. Finally, the solution collected through the rubber-headed tube has poor accuracy. Utility Model Content

[0006] In view of the problems existing in the prior art, the purpose of the present invention is to provide an antigen cell collection device, which can more accurately collect cells in the interface layer through the collection component, thereby improving the collection accuracy and solving the background technology problems.

[0007] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0008] An antigen cell collection device comprises a collection barrel, a sealing cover is threadedly connected to the top of the collection barrel, a liquid inlet is provided on the top of the sealing cover, a centrifugal barrel is rotatably connected to the inner wall of the collection barrel, an outer gear ring is fixedly connected to the bottom of the centrifugal barrel, a centrifugal motor is installed on the right side of the collection barrel, a gear is fixedly connected to the output shaft of the centrifugal motor, a through slot is provided on the right side of the collection barrel, the gear passes through the through slot and meshes with the outer gear ring, a guide funnel is fixedly connected to the inner wall of the collection barrel, the bottom end of the guide funnel passes through and extends into the interior of the centrifugal barrel, and a collection assembly is provided on the inner wall of the collection barrel;

[0009] The collection component includes a liquid pump, which is installed on the inner wall of the collection tube. A hose is connected to the liquid pump, and a collection needle is installed at the other end of the hose. A push rod is fixedly installed at the bottom of the guide funnel, and the bottom end of the push rod is connected to a supporting plate. The collection needle is installed on the supporting plate. A through hole is opened at the bottom of the centrifuge tube, and the collection needle is located at the top of the through hole.

[0010] As a further description of the above technical solution: a processor is integrated inside the collection tube, a liquid level sensor is installed on the inner wall of the centrifugal tube, and a display controller is fixedly installed on the outside of the collection tube. The display controller, liquid level sensor, centrifugal motor and collection component are all connected to the processor signal.

[0011] As a further description of the above technical solution: a status indicator light is installed on the collection tube, and the status indicator light is connected to the processor signal.

[0012] As a further description of the above technical solution: a protective cover is movably installed on the right side of the collection tube, and the centrifugal motor is located inside the protective cover.

[0013] As a further description of the above technical solution: the hose is located on the top of the carrying plate.

[0014] As a further description of the above technical solution: a filter plate is detachably connected to the inner wall of the sealing cover, and the filter plate is located at the bottom of the liquid inlet.

[0015] Compared with the prior art, the advantages of the present invention are:

[0016] In the present invention, a sealed centrifuge cylinder is provided to centrifuge a dendritic cell solution to which a medium is added, so that a good separation effect is achieved and cells to be collected are more easily obtained. Furthermore, the height of the solution inside the centrifuge cylinder can be understood through the cooperation of a liquid level sensor and a display controller. Cells in the interface layer can be collected more accurately through a collection component, thereby improving collection accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the three-dimensional structure of the utility model;

[0018] Figure 2 This is a schematic diagram of the front cross-sectional structure of the present utility model;

[0019] Figure 3 For the utility model Figure 2 A in the middle is an enlarged structural diagram;

[0020] Figure 4 It is a schematic diagram of the principle of the present utility model.

[0021] Description of the numbers in the figure:

[0022] 1. Collection tube; 2. Sealing cover; 3. Liquid inlet; 4. Centrifuge tube; 5. External gear ring; 6. Centrifugal motor; 7. Guide funnel; 8. Collection assembly; 801. Liquid pump; 802. Hose; 803. Collection needle; 804. Push rod; 805. Carrying plate; 806. Through hole; 9. Processor; 10. Liquid level sensor; 11. Display controller; 12. Status indicator light; 13. Protective cover; 14. Filter plate. DETAILED DESCRIPTION

[0023] The technical solutions in the embodiments of the present invention will be described clearly and completely below with reference to the accompanying drawings in the embodiments of the present invention.

[0024] See also Figures 1 to 4 In the utility model, an antigen cell collection device includes a collection tube 1, a sealing cover 2 is threadedly connected to the top of the collection tube 1, a liquid inlet 3 is opened on the top of the sealing cover 2, a centrifugal tube 4 is rotatably connected to the inner wall of the collection tube 1, an outer gear ring 5 is fixedly connected to the bottom of the centrifugal tube 4, a centrifugal motor 6 is installed on the right side of the collection tube 1, a gear is fixedly connected to the output shaft of the centrifugal motor 6, a through groove is opened on the right side of the collection tube 1, the gear passes through the through groove and meshes with the outer gear ring 5, a guide funnel 7 is fixedly connected to the inner wall of the collection tube 1, the bottom end of the guide funnel 7 passes through and extends to the interior of the centrifugal tube 4, and a collection component 8 is provided on the inner wall of the collection tube 1.

[0025] The collection component 8 includes a liquid suction pump 801, which is installed on the inner wall of the collection tube 1. A hose 802 is connected to the liquid suction pump 801, and a collection needle 803 is installed at the other end of the hose 802. A push rod 804 is fixedly installed at the bottom of the guide funnel 7, and the bottom end of the push rod 804 is connected to a supporting plate 805. The collection needle 803 is installed on the supporting plate 805. A through hole 806 is opened at the bottom of the centrifuge tube 4, and the collection needle 803 is located at the top of the through hole 806.

[0026] A processor 9 is integrated inside the collection tube 1, a liquid level sensor 10 is installed on the inner wall of the centrifugal tube 4, and a display controller 11 is fixedly installed on the outside of the collection tube 1. The display controller 11, liquid level sensor 10, centrifugal motor 6 and collection component 8 are all connected to the processor 9 for signal.

[0027] The hose 802 is located on the top of the carrier plate 805; the filter plate 14 is detachably connected to the inner wall of the sealing cover 2, and the filter plate 14 is located at the bottom of the liquid inlet 3;

[0028] As can be seen in the figure, the other side of the liquid pump 801 is connected to a liquid outlet pipe, and the bottom of the centrifugal cylinder 4 is connected to a liquid discharge pipe that runs through the bottom of the collection cylinder 1. The liquid discharge pipe is rotatably connected to the collection cylinder 1.

[0029] When it is necessary to collect antigen cells, the user adds a solution containing dendritic cells, such as blood or bone marrow, to the medium through the top liquid inlet 3. At this time, the solution moves downward to penetrate the filter plate 14, and the filter plate 14 filters the impurities in the solution. Then the solution continues to move downward to the inside of the guide funnel 7 and enters the centrifugal cylinder 4. At this time, the liquid level sensor 10 monitors the liquid level of the solution and transmits it to the processor 9. After receiving the signal, the processor 9 displays the value on the display controller 11. Then, the centrifugal motor 6 can be controlled to work, and the centrifugal motor 6 drives the gear fixed to it to rotate. The outer gear ring 5 of the gear starts to rotate, and the centrifugal cylinder 4 rotates to make the dendritic cells inside. The solution begins to separate. The density gradient medium is used to separate cells according to their density. After centrifugation is completed, the centrifugal motor 6 stops, and the interior of the centrifuge cylinder 4 is layered, with the medium at the bottom, the dendritic cells in the middle, and the plasma or cell culture supernatant at the top. The liquid level sensor 10 can determine the height of the interface layer based on the liquid level position, and the control push rod 804 drives the supporting plate 805 fixed to it to move downward. The collection needle 803 then moves downward and penetrates the through hole 806 until it enters the interface layer. The liquid pump 801 then operates to extract the dendritic cells in the interface layer through the hose 802 to complete the collection. The collection assembly 8 is then reset, and the bottom drain pipe is opened to discharge the remaining liquid.

[0030] In the present invention, a sealed centrifuge cylinder 4 is provided to centrifuge the dendritic cell solution to which a medium is added, so that the separation effect is good and the cells to be collected are more easily obtained. Moreover, the height of the solution inside the centrifuge cylinder 4 can be understood through the cooperation of the liquid level sensor 10 and the display controller 11. The cells in the interface layer can be collected more accurately through the collection component 8, thereby improving the collection accuracy.

[0031] See also Figure 1 and 4 , wherein: a status indicator light 12 is installed on the collection tube 1, and the status indicator light 12 is connected to the processor 9 signal.

[0032] In the present invention, the processor 9 controls the status indicator light 12 to flash. For example, after reaching the collection depth, the processor 9 controls the status indicator light 12 to light up, so as to prompt the user to collect cells.

[0033] See also Figure 2 , wherein: a protective cover 13 is movably installed on the right side of the collection tube 1, and the centrifugal motor 6 is located inside the protective cover 13.

[0034] In the present invention, the provision of the protective cover 13 can protect the centrifugal motor 6 and prevent the centrifugal motor 6 from being hit, while also improving the appearance.

[0035] The above description is merely a preferred embodiment of the present invention; however, the scope of protection of the present invention is not limited thereto. Any person skilled in the art who, within the technical scope disclosed in the present invention, makes equivalent substitutions or modifications based on the technical solutions and improved concepts of the present invention shall be covered by the scope of protection of the present invention.

Claims

1. An antigen cell collection device, comprising a collection tube (1), characterized in that: The top of the collecting tube (1) is threadedly connected to a sealing cover (2), the top of the sealing cover (2) is provided with a liquid inlet (3), the inner wall of the collecting tube (1) is rotatably connected to a centrifugal tube (4), the bottom of the centrifugal tube (4) is fixedly connected to an outer toothed ring (5), a centrifugal motor (6) is installed on the right side of the collecting tube (1), a gear is fixedly connected to the output shaft of the centrifugal motor (6), a through groove is provided on the right side of the collecting tube (1), the gear passes through the through groove and meshes with the outer toothed ring (5), a guide funnel (7) is fixedly connected to the inner wall of the collecting tube (1), the bottom end of the guide funnel (7) passes through and extends to the inside of the centrifugal tube (4), and a collecting component (8) is provided on the inner wall of the collecting tube (1); The collection assembly (8) comprises a liquid pump (801), the liquid pump (801) is mounted on the inner wall of the collection tube (1), the liquid pump (801) is connected to a hose (802), a collection needle (803) is mounted on the other end of the hose (802), a push rod (804) is fixedly mounted on the bottom of the guide funnel (7), the bottom end of the push rod (804) is connected to a supporting plate (805), the collection needle (803) is mounted on the supporting plate (805), a through hole (806) is opened at the bottom of the centrifugal tube (4), and the collection needle (803) is located at the top of the through hole (806).

2. The antigen cell collection device according to claim 1, characterized in that: A processor (9) is integrated inside the collection tube (1), a liquid level sensor (10) is installed on the inner wall of the centrifugal tube (4), and a display controller (11) is fixedly installed on the outer side of the collection tube (1). The display controller (11), the liquid level sensor (10), the centrifugal motor (6) and the collection component (8) are all connected to the processor (9) by signals.

3. The antigen cell collection device according to claim 2, characterized in that: The collecting tube (1) is provided with a status indicator light (12), and the status indicator light (12) is connected to the processor (9) by signal.

4. The antigen cell collection device according to claim 1, characterized in that: A protective cover (13) is movably mounted on the right side of the collecting tube (1), and the centrifugal motor (6) is located inside the protective cover (13).

5. The antigen cell collection device according to claim 1, characterized in that: The hose (802) is located on top of the carrying plate (805).

6. The antigen cell collection device according to claim 1, characterized in that: A filter plate (14) is detachably connected to the inner wall of the sealing cover (2), and the filter plate (14) is located at the bottom of the liquid inlet (3).

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