Rapid plant DNA (Deoxyribose Nucleic Acid) direct expansion sampler

Abstract

The utility model discloses a rapid plant DNA direct expansion sampler which comprises a sampling protection shell, the right side face of the sampling protection shell is fixedly communicated with a sliding pipe, the inner wall of the sliding pipe is in sliding connection with a connecting rod, the inner wall of the sampling protection shell is in sliding connection with a piston plate, and the piston plate is in sliding connection with the sliding pipe. And the right side face of the piston plate is fixedly connected with the left end of a connecting rod, the left side face of the sampling protection shell fixedly communicates with a liquid pumping pipe, and the right end of the liquid pumping pipe fixedly communicates with a rubber hose. Through cooperation of a connecting rod and a piston plate, air pressure in the sampling protective shell can be reduced, then a rubber balloon is expanded to suck plant grinding fluid, the grinding fluid enters a storage pipe, a rubber pressing head can move downwards through threaded connection of a threaded rod and a threaded pipe, and the plant grinding fluid can be taken out of the storage pipe. The rubber pressing head is matched with the top seat to extrude the rubber hose, so that a sample is in a closed environment and is prevented from being interfered by the outside, and accurate DNA data detection is ensured.

Description

Technical Field

[0001] The utility model relates to the technical field of plant gene sampling, in particular to a rapid plant DNA direct amplification sampler. Background Art

[0002] Plant genetic engineering technology is to first isolate a specific target gene, and then use a vector, media or other physical or chemical methods to introduce the isolated target gene into the plant cell receptor and integrate it into the chromosome of the plant receptor cell (the carrier of the gene), so as to obtain the expression of the target gene in the plant receptor, and ultimately achieve the purpose of changing plant traits (such as disease resistance, insect resistance, stress resistance, etc.) and quickly breeding new plant varieties.

[0003] In the process of extracting plant genes, plant DNA is generally extracted by the extraction-free DNA direct amplification method. During the extraction process, a sampler is needed to sample the grinding liquid. Although the current sampler can be used for sampling, during the use process, since the DNA is difficult to be in a closed environment after sampling, the DNA is easily affected by the outside world, resulting in inaccurate DNA data detection. Utility Model Content

[0004] The utility model aims to solve the shortcomings in the prior art and proposes a rapid plant DNA direct amplification sampler.

[0005] In order to achieve the above-mentioned purpose, the utility model adopts the following technical solutions: a rapid plant DNA direct amplification sampler, comprising a sampling protective shell, the right side of the sampling protective shell is fixedly connected to a sliding tube, the inner wall of the sliding tube is slidably connected to a connecting rod, the inner wall of the sampling protective shell is slidably connected to a piston plate, the right side of the piston plate is fixedly connected to the left end of the connecting rod, the left side of the sampling protective shell is fixedly connected to a liquid suction tube, the right end of the liquid suction tube is fixedly connected to a rubber hose, the right end of the rubber hose is fixedly connected to a storage tube, the right end of the storage tube is fixedly connected to a rubber balloon, the upper surface of the sampling protective shell is fixedly connected to a sealing shell, the upper surface of the sealing shell is rotatably connected to a threaded rod through a sealing bearing, the outer surface of the threaded rod is threadedly connected to a threaded tube, the bottom end of the threaded tube is fixedly connected to a rubber pressure head, and the inner bottom wall of the sampling protective shell is fixedly connected to a top seat.

[0006] As a further description of the above technical solution:

[0007] A connecting ring is fixedly connected to the left side of the sampling protection shell, and a sealing cover is threadedly connected to the outer surface of the connecting ring.

[0008] As a further description of the above technical solution:

[0009] The left and right side surfaces of the sealed shell are fixedly connected with reinforcement blocks, and the bottom surface of each reinforcement block is fixedly connected to the upper surface of the sampling protection shell.

[0010] As a further description of the above technical solution:

[0011] A fixing ring is fixedly connected to the outer surface of the sliding tube, and the left side of the fixing ring is fixedly connected to the right side of the sampling protection shell.

[0012] As a further description of the above technical solution:

[0013] The outer surface of the connecting rod is fixedly connected with a support seat, and the left side of the support seat is fixedly connected to the right side of the piston plate.

[0014] As a further description of the above technical solution:

[0015] The outer surface of the storage tube is sleeved with a limiting ring, the outer surface of the limiting ring is fixedly connected to a connecting block, and the bottom surface of the connecting block is fixedly connected to the inner bottom wall of the sampling protection shell.

[0016] The utility model has the following beneficial effects:

[0017] 1. Compared with the existing technology, this rapid plant DNA direct amplification sampler can reduce the air pressure inside the sampling protective shell by cooperating with a connecting rod and a piston plate, thereby expanding the rubber balloon to absorb the plant grinding liquid, allowing the grinding liquid to enter the interior of the storage tube. The threaded connection between the threaded rod and the threaded tube can move the rubber pressure head downward, so that the rubber pressure head cooperates with the top seat to squeeze the rubber hose, thereby placing the sample in a closed environment, preventing external interference, and ensuring accurate DNA data detection.

[0018] 2. Compared with the existing technology, this rapid plant DNA direct amplification sampler can protect the liquid extraction tube by using the cooperation of the sealing cover and the connecting ring to prevent dust from falling on the liquid extraction tube, and can support the piston plate by using the support seat to prevent the piston plate from deforming during the left and right sliding process, causing gas leakage. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a schematic diagram of the three-dimensional structure of a rapid plant DNA direct amplification sampler proposed in the utility model;

[0020] Figure 2 This is a schematic diagram of the three-dimensional structure of a top view of a rapid plant DNA direct amplification sampler proposed in the utility model;

[0021] Figure 3This is a schematic diagram of the three-dimensional structure of the front cross-section of a rapid plant DNA direct amplification sampler proposed by the utility model;

[0022] Figure 4 This is a schematic diagram of the three-dimensional structure of a side section view of a rapid plant DNA direct amplification sampler proposed by the utility model.

[0023] Legend:

[0024] 1. Sampling protective shell; 2. Threaded rod; 3. Sealing cover; 4. Reinforcement block; 5. Sealing shell; 6. Fixing ring; 7. Sliding tube; 8. Connecting rod; 9. Liquid extraction tube; 10. Connecting ring; 11. Rubber hose; 12. Top seat; 13. Storage tube; 14. Connecting block; 15. Rubber balloon; 16. Piston plate; 17. Limiting ring; 18. Support seat; 19. Threaded tube; 20. Rubber pressure head. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0026] Reference Figure 1-4 The utility model provides a rapid plant DNA direct amplification sampler, including a sampling protection shell 1, the right side of the sampling protection shell 1 is fixedly connected with a sliding tube 7, the inner wall of the sliding tube 7 is slidably connected with a connecting rod 8, the inner wall of the sampling protection shell 1 is slidably connected with a piston plate 16, the right side of the piston plate 16 is fixedly connected to the left end of the connecting rod 8, the left side of the sampling protection shell 1 is fixedly connected with a liquid extraction tube 9, the right end of the liquid extraction tube 9 is fixedly connected with a rubber hose 11, the right end of the rubber hose 11 is fixedly connected with a storage tube 13, and the right end of the storage tube 13 is fixedly connected with a rubber balloon 15, the upper surface of the sampling protection shell 1 is fixedly connected with a sealing shell 5, the upper surface of the sealing shell 5 is rotatably connected with a threaded rod 2 through a sealing bearing, the outer surface of the threaded rod 2 is threadedly connected with a threaded tube 19, the bottom end of the threaded tube 19 is fixedly connected with a rubber pressure head 20, and the inner bottom wall of the sampling protection shell 1 is fixedly connected with a top seat 12.

[0027] In this embodiment, a connecting ring 10 is fixedly connected to the left side of the sampling protective shell 1, and a sealing cover 3 is threadedly connected to the outer surface of the connecting ring 10. The connecting ring 10 and the sealing cover 3 can be used to seal the liquid extraction tube 9 to prevent dust from being adsorbed on the liquid extraction tube 9. The left and right side surfaces of the sealing shell 5 are fixedly connected with reinforcement blocks 4. The bottom surface of each reinforcement block 4 is fixedly connected to the upper surface of the sampling protective shell 1. The reinforcement blocks 4 can increase the stability of the sealing shell 5 and prevent the sealing shell 5 from loosening and shaking. The outer surface of the sliding tube 7 is fixedly connected with a fixing ring 6. The left side of the fixing ring 6 is fixedly connected to the right side of the sampling protective shell 1. The fixing ring 6 can be used to reinforce the sliding tube 7, increase the stability of the sliding tube 7, and prevent the sliding tube 7 from loosening.

[0028] In this embodiment, the outer surface of the connecting rod 8 is fixedly connected to a support seat 18, and the left side of the support seat 18 is fixedly connected to the right side of the piston plate 16. The support seat 18 can support the piston plate 16 to prevent the piston plate 16 from being deformed during use, thereby ensuring that the air pressure inside the sampling protective shell 1 can be reduced. The outer surface of the storage tube 13 is sleeved with a limiting ring 17, and the outer surface of the limiting ring 17 is fixedly connected to a connecting block 14. The bottom surface of the connecting block 14 is fixedly connected to the inner bottom wall of the sampling protective shell 1. The limiting ring 17 and the connecting block 14 can limit the storage tube 13 to prevent the storage tube 13 from swinging, which makes it difficult to discharge the sample.

[0029] Working principle: When in use, rotate the sealing cover 3 to separate the sealing cover 3 from the connecting ring 10, then make the liquid extraction tube 9 contact with the sample, pull the connecting rod 8, so that the connecting rod 8 drives the piston plate 16 to move, and the piston plate 16 moves to reduce the air pressure inside the sampling protective shell 1, thereby expanding the rubber balloon 15, causing the liquid extraction tube 9 and the rubber hose 11 to extract the sample, and allowing the sample to enter the interior of the storage tube 13, and rotate the threaded rod 2 so that the threaded rod 2 is connected to the threaded tube 19 through the thread, pushing the rubber pressure head 20 to move downward, and the rubber pressure head 20 moves downward to squeeze the rubber hose 11, and cooperates with the top seat 12 to seal the rubber hose 11, thereby preventing the sample from being disturbed by the outside world.

[0030] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent replacements for some of the technical features therein. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A rapid plant DNA direct amplification sampler, comprising a sampling protective housing (1), characterized in that: The right side surface of the sampling protection shell (1) is fixedly connected to a sliding tube (7), the inner wall of the sliding tube (7) is slidably connected to a connecting rod (8), the inner wall of the sampling protection shell (1) is slidably connected to a piston plate (16), the right side surface of the piston plate (16) is fixedly connected to the left end of the connecting rod (8), the left side surface of the sampling protection shell (1) is fixedly connected to a liquid extraction tube (9), the right end of the liquid extraction tube (9) is fixedly connected to a rubber hose (11), and the right end of the rubber hose (11) is fixedly connected to A storage tube (13) is provided, the right end of which is fixedly connected to a rubber balloon (15), the upper surface of the sampling protection shell (1) is fixedly connected to a sealing shell (5), the upper surface of the sealing shell (5) is rotatably connected to a threaded rod (2) via a sealing bearing, the outer surface of the threaded rod (2) is threadedly connected to a threaded tube (19), the bottom end of the threaded tube (19) is fixedly connected to a rubber pressure head (20), and the inner bottom wall of the sampling protection shell (1) is fixedly connected to a top seat (12).

2. A rapid plant DNA direct amplification sampler according to claim 1, characterized in that: A connecting ring (10) is fixedly connected to the left side surface of the sampling protection housing (1), and a sealing cover (3) is threadedly connected to the outer surface of the connecting ring (10).

3. A rapid plant DNA direct amplification sampler according to claim 1, characterized in that: The left and right side surfaces of the sealed shell (5) are fixedly connected with reinforcement blocks (4), and the bottom surface of each reinforcement block (4) is fixedly connected to the upper surface of the sampling protection shell (1).

4. A rapid plant DNA direct amplification sampler according to claim 1, characterized in that: A fixing ring (6) is fixedly connected to the outer surface of the sliding tube (7), and the left side surface of the fixing ring (6) is fixedly connected to the right side surface of the sampling protection housing (1).

5. A rapid plant DNA direct amplification sampler according to claim 1, characterized in that: The outer surface of the connecting rod (8) is fixedly connected to a support seat (18), and the left side surface of the support seat (18) is fixedly connected to the right side surface of the piston plate (16).

6. A rapid plant DNA direct amplification sampler according to claim 1, characterized in that: The outer surface of the storage tube (13) is sleeved with a limiting ring (17), the outer surface of the limiting ring (17) is fixedly connected to a connecting block (14), and the bottom surface of the connecting block (14) is fixedly connected to the inner bottom wall of the sampling protection housing (1).

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