Cell activity detection kit

Abstract

The utility model relates to the technical field of kits, in particular to a cell activity detection kit. In order to solve the problems that the time interval between the first hole for adding the reagent and the last hole for adding the reagent is too long, so that the detection result is difficult to directly compare and the accuracy of the detection result is influenced by sequentially adding the reagent into each hole by adopting a pipettor, the following technical scheme is provided: the kit comprises a kit body, a plurality of groups of storage holes which are distributed at equal intervals and are used for storing cells are formed in the kit body, and a sliding groove formed in the top of the kit body is formed in one side of each group of storage holes; and each group of liquid storage pipes are correspondingly arranged in the multiple groups of sliding grooves, and the bottoms of the liquid storage pipes are attached to the sliding grooves. According to the utility model, a detection reagent can be pre-stored and then synchronously added, so that the consistency of reaction time in a plurality of groups of storage holes is ensured, and the detection result has extremely high accuracy.

Description

Technical Field

[0001] The utility model relates to the technical field of kits, in particular to a cell viability detection kit. Background Art

[0002] Cell viability detection is not only widely used in scientific research, but also has important significance in the fields of drug development, disease research and toxicological evaluation, providing scientists with key tools for evaluating cell function and physiological state. When detecting cell viability, the cells are first evenly distributed to different wells in the test kit, each well may be subjected to different treatments or drug treatments with a concentration gradient, and then pre-diluted detection reagents are added to each well. At present, a pipette is used to add reagents to each well in turn, which may cause the interval between the first well to add the reagent and the last well to add the reagent to be too long, so that the test results are difficult to directly compare, affecting the accuracy of the test results. In view of this, the utility model proposes a cell viability detection kit. Utility Model Content

[0003] The utility model aims to propose a cell viability detection kit to address the problem in the background technology that a pipette is used to add reagents to each well in sequence, which may result in a long interval between the first well to add the reagent and the last well to add the reagent, making it difficult to directly compare the test results, thereby affecting the accuracy of the test results.

[0004] The technical solution of the utility model is as follows: a cell viability detection kit, comprising a kit body, the kit body is provided with a plurality of groups of equally spaced storage holes for storing cells, and one side of each group of the storage holes is provided with a slide groove opened on the top of the kit body; a plurality of groups of liquid storage tubes, each group of the liquid storage tubes is correspondingly arranged in a plurality of slide grooves, and the bottom of the liquid storage tubes is in contact with the slide grooves; a shifting component arranged on the top of the kit body, the shifting component includes a mounting frame arranged on the top of the kit body, and the plurality of groups of the liquid storage tubes are fixedly arranged in the mounting frame; a plurality of clamping mechanisms installed on the kit body for driving the liquid storage tubes to be pressed tightly in the slide grooves, the clamping mechanisms include an L-shaped pressing block slidably connected to the kit body, and one side of the pressing block is provided with a fixing rod opened on the mounting frame.

[0005] Optionally, the displacement assembly further includes a fixing ring and a fixing rod, each group of the liquid storage tubes is fixedly connected with a fixing ring, two adjacent groups of fixing rings are fixedly connected with a fixing rod, and a fixing rod is also fixedly connected between the fixing ring close to the mounting frame and the mounting frame.

[0006] Optionally, the clamping mechanism also includes a movable plate, a sliding rod, and a limit plate. Both sides of the bottom of the pressing block are fixedly connected to the movable plate, and a sliding rod is installed on the top of the movable plate. The sliding rod passes through one end of the reagent box body and is fixedly connected to the limit plate.

[0007] Optionally, the clamping mechanism further comprises a spring sleeved on the outer ring of the slide rod, and the spring is arranged between the movable plate and the reagent box body.

[0008] Optionally, it also includes a pushing component installed on one side of the installation frame to prevent the installation frame from accidentally moving.

[0009] Optionally, the pushing assembly is arranged on a side of the fixing rod away from a group of pressing blocks corresponding thereto.

[0010] Optionally, the pushing assembly includes a push plate, a card plate, and a positioning block. The push plate is fixedly connected to one side of the installation frame. Multiple groups of card plates are fixedly connected to the side of the push plate opposite to the reagent box body. The multiple groups of card plates are staggered, and the positioning block is slidably connected between two groups of card plates.

[0011] Compared with the prior art, the utility model has the following beneficial technical effects:

[0012] The utility model sets a pressing mechanism, and the spring releases elastic force to make the pressing block move downward, so that the installation frame can drive the liquid storage tube to be pressed tightly in the slide groove, so that the detection reagent can be added to the liquid storage tube, so that the reagent is retained in the liquid storage tube without contacting the cells in the storage hole;

[0013] Further, by setting the push assembly, after the positioning block is pulled out, the push plate can be pushed and the installation frame can be driven to move, so that the bottom of the liquid storage tube slides in the slide groove and moves above the storage hole, so that the detection reagents in all liquid storage tubes enter the storage hole synchronously, ensuring the consistency of the reaction time, thereby improving the accuracy of the detection result;

[0014] In summary, the utility model can pre-store the detection reagents and then add them synchronously, thereby ensuring the consistency of reaction time in multiple groups of storage wells, and the detection results have extremely high accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 A schematic diagram of the structure of a cell viability detection kit is provided;

[0016] Figure 2 It is a schematic diagram of the structure of the kit itself;

[0017] Figure 3 It is a schematic diagram of a partial cross-sectional structure of the test kit body;

[0018] Figure 4 for Figure 1 The enlarged schematic diagram of point A in the middle;

[0019] Figure 5 It is a structural diagram of the push component.

[0020] Reference numerals:

[0021] 1. Reagent box body; 11. Storage hole; 12. Slide slot;

[0022] 2. Shifting assembly; 21. Mounting frame; 22. Fixing ring; 23. Fixing rod;

[0023] 3. Clamping mechanism; 31. Pressing block; 32. Moving plate; 33. Sliding rod; 34. Limiting plate; 35. Spring;

[0024] 4. Pushing assembly; 41. Pushing plate; 42. Card plate; 43. Positioning block;

[0025] 5. Liquid storage tube. DETAILED DESCRIPTION

[0026] The technical solution of the present utility model will be clearly and completely described below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present utility model, rather than all of the embodiments.

[0027] The components of the embodiments of the present invention generally described and shown in the drawings herein can be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the claimed invention, but merely represents selected embodiments of the present invention.

[0028] Based on the embodiments of the present utility model, all other embodiments obtained by ordinary technicians in the field without making any creative work shall fall within the scope of protection of the present utility model.

[0029] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0030] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0031] Example

[0032] like Figure 1 and Figure 2 As shown, a cell viability detection kit proposed in the utility model includes a kit body 1, on which a plurality of equally spaced storage holes 11 for storing cells are arranged, and one side of each group of storage holes 11 is provided with a slide groove 12 opened on the top of the kit body 1.

[0033] For details, please refer to Figure 3 The above-mentioned test kit includes multiple groups of liquid storage tubes 5, each group of liquid storage tubes 5 is correspondingly arranged in multiple groups of slide grooves 12, and the bottom of the liquid storage tube 5 is in contact with the slide groove 12, so that after the detection reagent is placed in the liquid storage tube 5, the bottom opening of the liquid storage tube 5 is blocked by the slide groove 12, so that the reagent in the liquid storage tube 5 will not flow out.

[0034] Further, such as Figure 4 As shown, the above-mentioned test kit also includes a shifting component 2 arranged on the top of the test kit body 1, and the shifting component 2 includes a mounting frame 21 arranged on the top of the test kit body 1, and multiple groups of liquid storage tubes 5 are fixedly arranged in the mounting frame 21. The shifting component 2 also includes a fixing ring 22 and a fixing rod 23. Each group of liquid storage tubes 5 is fixedly connected with a fixing ring 22, and a fixing rod 23 is fixedly connected between two adjacent groups of fixing rings 22. The fixing ring 22 close to the mounting frame 21 is also fixedly connected to the mounting frame 21 with a fixing rod 23, so that the mounting frame 21 can simultaneously drive multiple groups of liquid storage tubes 5 to move when moving.

[0035] Furthermore, if Figure 4As shown, the reagent box includes a plurality of clamping mechanisms 3 installed on the reagent box body 1 for driving the liquid storage tube 5 to be pressed tightly in the slide groove 12. The clamping mechanism 3 includes an L-shaped clamping block 31 slidably connected to the reagent box body 1. The clamping block 31 is downwardly moved by the elastic force of the spring 35, thereby pressing the installation frame 21 downward, so that the bottom of the liquid storage tube 5 is pressed tightly in the slide groove 12. A fixed rod 23 opened on the installation frame 21 is provided on one side of the clamping block 31. After the installation frame 21 moves, when the fixed rod 23 moves to a position corresponding to the clamping block 31, the clamping block 31 moves downward and no longer presses the installation frame 21, so that the reagent in the liquid storage tube 5 can flow out from the bottom. The clamping mechanism 3 also includes a moving plate 32, a sliding rod 33, and a limiting plate 34. The bottom sides of the clamping block 31 are fixedly connected with the moving plate 32, and the moving plate 32 moves synchronously with the clamping block 31. A slide bar 33 is installed on the top of the moving plate 32. One end of the slide bar 33 passes through the reagent box body 1 and is fixedly connected to a limit plate 34, which is used to limit the moving plate 32 and prevent the moving plate 32 from falling. The clamping mechanism 3 also includes a spring 35 sleeved and installed on the outer ring of the slide bar 33. The spring 35 is arranged between the moving plate 32 and the reagent box body 1. The spring 35 is used to release elastic force to drive the pressing block 31 to press down the installation frame 21, so that the liquid storage tube 5 is pressed in the slide groove 12.

[0036] Finally, if Figure 5 As shown, it also includes a pushing assembly 4 installed on one side of the installation frame 21 to prevent the installation frame 21 from accidentally moving. The pushing assembly 4 is arranged on the side of the fixed rod 23 away from a group of pressing blocks 31 corresponding thereto. The pushing assembly 4 includes a push plate 41, a card plate 42, and a positioning block 43. The push plate 41 is fixedly connected to one side of the installation frame 21. When the push plate 41 moves, it drives the installation frame 21 to move synchronously, so as to facilitate the movement of the installation frame 21. The push plate 41 is fixedly connected to a side opposite to the reagent box body 1 with multiple groups of card plates 42, and the multiple groups of card plates 42 are staggered. The positioning block 43 is slidably connected between two groups of card plates 42, and is used to support the push plate 41 to prevent the push plate 41 from moving due to accidental touch and driving the installation frame 21 to move.

[0037] In this embodiment, cells are first added to the storage hole 11, and the detection reagent is added to all the liquid storage tubes 5 in sequence using a pipette. Then the positioning block 43 is pulled out, and the push plate 41 is pushed close to the reagent box body 1, and the push plate 41 drives the installation frame 21 to move synchronously. When the installation frame 21 moves, the fixing ring 22 is driven to move synchronously through multiple groups of fixing rods 23, so that multiple groups of liquid storage tubes 5 move synchronously, and the liquid storage tubes 5 slide in the chute 12. At this time, the liquid storage tubes 5 slowly move to the top of the storage hole 11, and the detection reagent in the liquid storage tubes 5 flows into the storage hole 11 and mixes with the cells, and the cells in the multiple groups of storage holes 11 are in synchronous contact with the detection reagent, so that the reaction time is equal. Until the installation frame 21 moves, the fixing rod 23 moves to the position of the pressing block 31, and the spring 35 releases the elastic force to make the pressing block 31 move downward. At this time, the spring 35 no longer presses the installation frame 21 downward, and the installation frame 21 can be lifted upward together with the fixing rod 23, the fixing ring 22 and the liquid storage tube 5, so as to facilitate the observation of the reagent box body 1, so as to obtain accurate detection results.

[0038] The above-mentioned specific embodiments are only several optional embodiments of the present invention. Based on the technical solution of the present invention and the relevant inspiration of the above-mentioned embodiments, those skilled in the art can make various alternative improvements and combinations to the above-mentioned specific embodiments.

Claims

1. A cell viability detection kit, characterized in that: include: A reagent box body (1), wherein the reagent box body (1) is provided with a plurality of equally spaced storage holes (11) for storing cells, and one side of each group of storage holes (11) is provided with a slide groove (12) opened at the top of the reagent box body (1); A plurality of groups of liquid storage tubes (5), each group of the liquid storage tubes (5) being correspondingly arranged in a plurality of groups of chutes (12), and the bottom of the liquid storage tubes (5) being in contact with the chutes (12); A displacement assembly (2) disposed on the top of the reagent box body (1), wherein the displacement assembly (2) comprises a mounting frame (21) disposed on the top of the reagent box body (1), and a plurality of groups of liquid storage tubes (5) are fixedly disposed in the mounting frame (21); A plurality of pressing mechanisms (3) are mounted on the reagent box body (1) and are used to drive the liquid storage tube (5) to be pressed tightly in the slide groove (12). The pressing mechanisms (3) include an L-shaped pressing block (31) slidably connected to the reagent box body (1), and a fixing rod (23) opened on the installation frame (21) is arranged on one side of the pressing block (31).

2. A cell viability detection kit according to claim 1, characterized in that: The displacement assembly (2) further comprises a fixing ring (22) and a fixing rod (23); each group of the liquid storage tubes (5) is fixedly connected with a fixing ring (22); a fixing rod (23) is fixedly connected between two adjacent groups of fixing rings (22); and a fixing rod (23) is also fixedly connected between the fixing ring (22) close to the installation frame (21) and the installation frame (21).

3. A cell viability detection kit according to claim 2, characterized in that: The clamping mechanism (3) further comprises a movable plate (32), a sliding rod (33) and a limiting disk (34); both sides of the bottom of the pressing block (31) are fixedly connected to the movable plate (32); a sliding rod (33) is installed on the top of the movable plate (32); one end of the sliding rod (33) passes through the reagent box body (1) and is fixedly connected to the limiting disk (34).

4. A cell viability detection kit according to claim 3, characterized in that: The clamping mechanism (3) further comprises a spring (35) sleeved and mounted on the outer ring of the sliding rod (33), and the spring (35) is arranged between the moving plate (32) and the reagent box body (1).

5. A cell viability detection kit according to claim 4, characterized in that: It also includes a pushing component (4) installed on one side of the installation frame (21) and used to prevent the installation frame (21) from accidentally moving.

6. A cell viability detection kit according to claim 5, characterized in that: The pushing assembly (4) is arranged on a side of the fixing rod (23) away from a group of pressing blocks (31) corresponding thereto.

7. A cell viability detection kit according to claim 6, characterized in that: The pushing assembly (4) comprises a pushing plate (41), a card plate (42), and a positioning block (43); the pushing plate (41) is fixedly connected to one side of the mounting frame (21); a plurality of groups of card plates (42) are fixedly connected to the side of the pushing plate (41) opposite to the reagent box body (1); the plurality of groups of card plates (42) are staggered; and the positioning block (43) is slidably connected between two groups of card plates (42).

Images