Cell culture fluid conveying device

Abstract

The utility model discloses a cell culture fluid conveying device, relates to the technical field of medical instruments, and provides the following scheme that the cell culture fluid conveying device comprises a shell, a cavity is formed in one side of the interior of the shell, a limiting baffle is arranged on the inner wall of the cavity, and a culture suite is installed below the limiting baffle; a supporting plate is fixed to the other side of the interior of the shell, a power unit and a sensor are installed in the shell and connected with the supporting plate, a pipeline suite is installed outside the supporting plate, a transfer bag suite is externally connected to the end of the pipeline suite, and a hook is installed at the top of the inner wall of the shell; the middle parts of the power unit and the sensor are connected with a sterile connector; the utility model aims to ensure the sterility of inlet and outlet liquid in the culture process through the cooperative work of a plurality of pipelines in the cell culture process; the operation difficulty in the processes of replacing or discharging waste liquid, extracting cells and inputting the cells during cell culture is reduced, and the success rate of cell culture is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of medical devices, in particular to a cell culture fluid delivery device. Background Art

[0002] During the cell culture process, cells are typically grown in closed vessels and covered with cell culture media, which provide the cells with essential nutrients and other supplements for growth. As cells grow and multiply, they consume nutrients in the cell culture fluid and produce waste byproducts. For this reason, solutions such as cell culture media must be regularly added and the waste fluid must be pumped out to allow the cells to continue growing.

[0003] The existing cell culture methods require manual replacement of the culture container interface connection when replacing the culture medium, extracting cells, and discharging waste liquid. This cannot ensure the sterility of the cell culture and is prone to cell contamination, leading to culture failure. Manual replacement of the culture container interface connection requires manual operation, which is difficult and time-consuming, and easily affects the timeliness of cell culture. Therefore, a cell culture fluid delivery device is needed. Utility Model Content

[0004] The utility model aims to provide a cell culture fluid delivery device, which solves the problem in the prior art that the existing cell culture method needs to manually replace the culture container interface connection when replacing the culture medium, extracting cells and discharging waste liquid, which cannot ensure the sterility of the cell culture, easily causes cell contamination and leads to culture failure; when manually replacing the culture container interface connection, manual operation is required, which is difficult and time-consuming, and easily affects the timeliness of cell culture.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a cell culture fluid delivery device, comprising a shell, a cavity is installed on one side of the interior of the shell, a limit baffle is provided on the inner wall of the cavity, a culture kit is installed below the limit baffle, a support plate is fixed to the other side of the interior of the shell, a power unit and a sensor are installed inside the shell and connected to the support plate, a pipeline kit is installed outside the support plate, a transfer bag kit is externally connected to the end of the pipeline kit, a hook is installed on the top of the inner wall of the shell, and a sterile connector is connected to the middle of the power unit and the sensor.

[0006] Preferably, the culture kit comprises a culture dish, a gas interface filter and a first connection part A, a side of the culture dish is connected to the gas interface filter, and the other side of the culture dish is connected to the first connection part A.

[0007] Preferably, the power unit and the sensor include a peristaltic pump, a bubble sensor and a tube clamp valve, the tube clamp valve is arranged on one side of the peristaltic pump, and the bubble sensor is arranged on the other side of the peristaltic pump.

[0008] Preferably, the pipeline kit includes a support clamp, a connecting pipeline is clamped and fixed in the middle of the support clamp, several groups of the support clamps and the connecting pipelines are arranged correspondingly, one of the several ends of the connecting pipeline is provided with a pressure interface filter, one of the several ends of the connecting pipeline is connected to the first connecting part B, one of the several ends of the connecting pipeline is connected to a sample bag, one of the several ends of the connecting pipeline is connected to a waste liquid bag, one of the several ends of the connecting pipeline is connected to a finished product bag, one of the several ends of the connecting pipeline is connected to the second connecting part A, one of the several ends of the connecting pipeline is connected to a liquid filter, the end of the gas interface filter is connected to a gas interface body, the end of the pressure interface filter is connected to a pressure interface body, a pipeline fixing block is arranged on one side of the support plate, and one of the several ends of the connecting pipeline is connected to an air filter.

[0009] Preferably, the transfer bag kit includes a cell transfer bag, a culture medium transfer bag is arranged on the side of the cell transfer bag, other transfer bags are arranged on the side of the culture medium transfer bag, a PBS transfer bag is arranged on the side of the other transfer bag, and the bottom of the cell transfer bag is connected to a second connecting part B.

[0010] Preferably, the aseptic connector is composed of a first connection part A, a first connection part B and a second connection part A and a second connection part B in corresponding combination.

[0011] Compared with the prior art, the beneficial effects of the utility model are:

[0012] 1. By setting up a culture kit, the culture kit is a closed culture environment, and the culture kit is connected to the pipeline kit and the transfer bag kit through a sterile connector; the culture dish is carried out in a closed system during the culture and centrifugation process, and liquid can be added and aspirated in real time under the working state, without stopping the culture, manual centrifugation, and manual liquid replacement. It is convenient and fast, reducing the difficulty of operation during the work process and the possibility of cell contamination during the work process; modular design, the consumables are modular, divided into culture kits, pipeline kits, and transfer bag kits; the equipment is also modular, divided into a culture chamber that provides temperature and rotation, a gas mixing box that provides a gas environment, and a power supply that provides liquid transfer power. Units and sensors; these modules can be used individually or in combination with each other; for example, the mixed gas provided by the mixing box can be used as the gas for culturing adherent cells in the cell factory and inflating them separately; for example, when using the same set of piping kits, replace the culture kits with other functions, or replace the transfer bags of different liquids during the culture process; for example, the power unit can be used as a liquid dispensing device to distribute a large amount of culture medium or cell fluid, etc.; the inlet and outlet liquid pipelines are independently arranged on the support plate and then placed on the equipment as a whole for operation, avoiding clutter caused by too many pipelines, and being able to clearly understand the role of each path, which is convenient for operation; during use, the liquid preparation plan can be quickly set according to the path.

[0013] 2. Equipped with multiple input ports and output ports, the division of labor is clear, and a variety of different liquids can be input or output without disassembly during the cell culture process; the operation is simple, ensuring the sterility of the liquid exchange process; equipped with an air input port with an air filter, it can transfer the remaining cells in the pipeline to the target while ensuring the sterility of the air input; equipped with a bubble sensor and a pressure sensor, it can detect whether there is liquid and pressure inside the pipeline kit during the liquid delivery process to ensure that the liquid is not blocked and safely delivered to the corresponding position; the liquid delivery is controlled by a peristaltic pump, the delivery speed is adjustable, and the delivery volume is more accurate; multiple tube clamp valves work together, the response is fast, and different tube clamp valves can be pre-set to open or close at the same time, which can quickly switch different working paths and reduce operation time and cost; the pipeline is easy to lay, and only the pipeline and each container need to be replaced after each use, reducing loss and cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a schematic diagram of the overall structure of a cell culture fluid delivery device of the utility model;

[0015] Figure 2 This is a schematic diagram of the overall installation structure of a support plate of a cell culture fluid delivery device of the utility model;

[0016] Figure 3This is a schematic diagram of the disassembled structure of a culture kit, a pipeline kit and a transfer bag kit of a cell culture fluid delivery device of the utility model;

[0017] Figure 4 The utility model is a schematic diagram of the overall process relationship structure of a cell culture fluid delivery device.

[0018] In the figure: 1. shell; 2. cavity; 3. limit baffle; 4. culture kit; 401. culture dish; 402. gas interface filter; 403. first connection part A; 5. support plate; 6. peristaltic pump; 7. support clamp; 8. connecting pipeline; 9. pressure interface filter; 10. first connection part B; 11. sample bag; 12. waste liquid bag; 13. finished product bag; 14. second connection part A; 15. liquid filter; 16. gas interface body; 17. pressure interface body; 18. pipeline fixing block; 19. bubble sensor; 20. tube clamp valve; 21. air filter; 22. transfer bag kit; 2201. cell transfer bag; 2202. culture medium transfer bag; 2203. other transfer bags; 2204. PBS transfer bag; 2205. second connection part B; 23. hook. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0020] Example 1

[0021] like Figure 1-4 As shown, a cell culture fluid delivery device in the figure includes a shell 1, which is characterized in that: a cavity 2 is installed on one side of the interior of the shell 1, a limit baffle 3 is provided on the inner wall of the cavity 2, a culture kit 4 is installed below the limit baffle 3, a support plate 5 is fixed on the other side of the interior of the shell 1, a power unit and a sensor are installed inside the shell 1 and connected to the support plate 5, a pipeline kit is installed outside the support plate 5, a transfer bag kit 22 is externally connected to the end of the pipeline kit, a hook 23 is installed on the top of the inner wall of the shell 1, and a sterile connector is connected to the middle of the power unit and the sensor.

[0022] The culture kit 4 includes a culture dish 401, a gas interface filter 402 and a first connection part A403. The side of the culture dish 401 is connected to the gas interface filter 402, and the other side of the culture dish 401 is connected to the first connection part A403. The two parts of the filter each contain a filter membrane, which can block the entry of bacteria. By connecting the two parts and clamping them, and then removing the filter membrane, the connection between the pipeline and the culture dish 401 is completed, allowing liquid to pass through and ensuring that no bacteria enter the entire connection process, ensuring the convenience of disassembly, assembly and replacement, and facilitating the guarantee of a sterile environment inside the culture dish 401.

[0023] The power unit and sensor include a peristaltic pump 6, a bubble sensor 19 and a tube clamp valve 20. The tube clamp valve 20 is arranged on the side of the peristaltic pump 6, and the bubble sensor 19 is arranged on the other side of the peristaltic pump 6. The power unit can be used as a liquid dispensing device to distribute a large amount of culture medium or cell fluid, and the sensor can effectively monitor the internal pressure and bubble conditions of multiple pipelines in real time.

[0024] The pipeline kit includes a support clamp 7, a connecting pipeline 8 is clamped and fixed in the middle of the support clamp 7, a plurality of groups of the support clamp 7 and the connecting pipeline 8 are arranged correspondingly, one of the plurality of ends of the connecting pipeline 8 is provided with a pressure interface filter 9, one of the plurality of ends of the connecting pipeline 8 is connected to a first connecting portion B10, one of the plurality of ends of the connecting pipeline 8 is connected to a sample bag 11, one of the plurality of ends of the connecting pipeline 8 is connected to a waste liquid bag 12, one of the plurality of ends of the connecting pipeline 8 is connected to a finished product bag 13, one of the plurality of ends of the connecting pipeline 8 is connected to a second connecting portion A14, and a plurality of ends of the connecting pipeline 8 are connected to a plurality of connecting portions A15. One of the ends is connected to a liquid filter 15, the end of the gas interface filter 402 is connected to a gas interface body 16, the end of the pressure interface filter 9 is connected to a pressure interface body 17, a pipeline fixing block 18 is provided on one side of the support plate 5, and one of the ends of the connecting pipeline 8 is connected to an air filter 21. The inlet and outlet liquid pipelines are independently arranged on the support plate 5 and then placed on the equipment as a whole for operation, so as to avoid clutter caused by too many pipelines, and the function of each path can be clearly understood, which is convenient for operation. During use, the liquid preparation plan can be quickly set according to the path.

[0025] The transfer bag kit 22 includes a cell transfer bag 2201, a culture medium transfer bag 2202 is arranged on the side of the cell transfer bag 2201, other transfer bags 2203 are arranged on the side of the culture medium transfer bag 2202, and PBS transfer bags 2204 are arranged on the side of other transfer bags 2203. The bottom of the cell transfer bag 2201 is connected to a second connecting part B2205. It is a modular design, and the consumables are modular, and the transfer bag kit 22 is one of them.

[0026] The sterile connector is composed of a first connection part A403, a first connection part B10, a second connection part A14, and a second connection part B2205. The two parts each contain a filter membrane that can block the entry of bacteria. By connecting the two parts and clamping them, and then removing the filter membrane, the connection between the pipeline and the culture dish 401 is completed, allowing liquid to pass through and ensuring that no bacteria enter the entire connection process.

[0027] Working principle: First, the liquid delivery device is equipped with multiple input ports for inputting culture medium, cell fluid and other liquids required for cell culture, and multiple output ports for sampling, collecting waste liquid, harvesting cells and other operations; the input end is equipped with an air input port equipped with an air filter 21, which is used to transport the remaining liquid in the pipeline to the target position. The device controls the closure of each liquid delivery pipeline through multiple tube clamp valves 20, and can control multiple tube clamp valves 20 to open or close at the same time, and freely combine to achieve the target pipeline flow; the liquid flow direction can be controlled by the bidirectional operation of the peristaltic pump 6, and the input or output can be switched freely; pressure sensors are installed at both ends of the peristaltic pump 6 to monitor the liquid pressure in the pipeline in real time and protect the pipeline; the culture container connection port is equipped with a bubble sensor 19 to monitor the bubble value in the pipeline and effectively protect the cell culture work;

[0028] Next, the power unit and sensor include a peristaltic pump 6, a bubble sensor 19 and a tube clamp valve 20. The peristaltic pump 6 provides liquid power, the tube clamp valve 20 is responsible for the flow direction of the liquid in the pipeline, and the bubble sensor 19 uses ultrasonic waves to detect whether there is liquid in the pipeline. Ultrasonic waves can ignore whether the pipeline is transparent, which improves the scope of use and accuracy. The photoelectric type needs to clarify the transparency of the pipeline, and an ultrasonic sensor is preferred; the pressure interface body 17 leads to the internal air pressure sensor, which is used to detect the internal pressure of the pipelines at both ends of the peristaltic pump 6 to prevent vacuumization or pipeline blockage. The gas interface body 16 leads to the internal mixing box, and the carbon dioxide mixed gas in the mixing box is output from this interface. The hook 23 is used to hang the transfer bag, and the pipeline fixing block 18 is used to fix the pipeline connecting the culture dish 401 and the pipeline kit. The limit baffle 3 limits the rotation of the fixed part of the rotary joint of the culture dish 401. The fixed part is connected to the pipeline kit, so its rotation must be limited. The limit baffle can be lifted 90 degrees to facilitate the placement of the culture dish 401.

[0029] Afterwards, the consumables are divided into three parts: a culture kit 4, a tubing kit, and a transfer bag kit 22; a sterile connector is used to connect the kits. Taking the first sterile connector as an example, the first sterile connector is divided into two parts: a first connection part A403 and a first connection part B10. The first connection part A403 is connected to the culture dish 401, and the first connection part B10 is connected to the connecting pipe 8 in the tubing kit. These two parts each contain a filter membrane that can block the entry of bacteria. By connecting the two parts and clamping them, and then removing the filter membrane, the connection between the pipeline and the culture dish 401 is completed, allowing liquid to pass through and ensuring that no bacteria enter the entire connection process.

[0030] Finally, the culture kit 4 includes a culture dish 401, a gas interface filter 402, and a first connection part A403 of a first sterile connector; the culture kit 4 is a closed small system, and the interior of the culture dish 401 is isolated from the external environment by a filter membrane or a filter; during assembly, the culture dish 401 is first loaded into the culture chamber of the machine, and the gas interface filter 402 is connected to the gas interface body 16, which is the interface for outputting the carbon dioxide mixed gas; the support plate 5 in the pipeline kit is a transparent plastic plate, and the shape of the opening on it is consistent with the power unit and the sensor. A number of pipeline support clamps 7 are installed on the support plate 5, and the connecting pipeline 8 is clamped in the pipeline support clamp 7. The support plate 5 can arrange the pipelines according to the function and can be easily installed on the machine as a whole; the liquid filter 15 in the pipeline kit is used to filter the liquid. For the liquid in the transfer bag, the pore size of the filter membrane is generally less than 0.2um and larger than the molecular size of the liquid to be filtered. The air filter 21 is used to filter the air in the external environment. When air is needed in the process of transferring liquid in the pipeline kit, it will enter the interior through this air filter 21; the pressure interface filter 9 is installed on the pressure interface body 17 to prevent the liquid in the pipeline from entering the pressure sensor; the pipeline kit is also a small closed system; the transfer bag kit 22 includes several transfer bags and a second connection part B2205, among which this case includes: cell transfer bag 2201, culture medium transfer bag 2202, other transfer bags 2203, PBS transfer bag 2204; among which the cell transfer 2201 bag must be connected to the second connection part B2205, that is, the cells must pass through the sterile connector but not through the liquid filter 15.

[0031] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0032] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cell culture fluid delivery device, comprising a housing (1), characterized in that: A cavity (2) is installed on one side of the interior of the shell (1), a limit baffle (3) is provided on the inner wall of the cavity (2), a culture kit (4) is installed below the limit baffle (3), a support plate (5) is fixed on the other side of the interior of the shell (1), a power unit and a sensor are installed inside the shell (1) and connected to the support plate (5), a pipeline kit is installed outside the support plate (5), and a transfer bag kit (22) is externally connected to the end of the pipeline kit, a hook (23) is installed on the top of the inner wall of the shell (1), and a sterile connector is connected to the middle of the power unit and the sensor.

2. A cell culture fluid delivery device according to claim 1, characterized in that: The culture kit (4) comprises a culture dish (401), a gas interface filter (402) and a first connection part A (403), wherein the side of the culture dish (401) is connected to the gas interface filter (402), and the other side of the culture dish (401) is connected to the first connection part A (403).

3. A cell culture fluid delivery device according to claim 1, characterized in that: The power unit and the sensor comprise a peristaltic pump (6), a bubble sensor (19) and a tube clamp valve (20); the tube clamp valve (20) is arranged on one side of the peristaltic pump (6), and the bubble sensor (19) is arranged on the other side of the peristaltic pump (6).

4. A cell culture fluid delivery device according to claim 2, characterized in that: The pipeline kit comprises a support clamp (7), a connecting pipeline (8) is clamped and fixed in the middle of the support clamp (7), a plurality of groups of the support clamp (7) and the connecting pipeline (8) are arranged correspondingly, a pressure interface filter (9) is arranged at one of the plurality of ends of the connecting pipeline (8), a first connecting portion B (10) is connected to one of the plurality of ends of the connecting pipeline (8), a sample bag (11) is connected to one of the plurality of ends of the connecting pipeline (8), a waste liquid bag (12) is connected to one of the plurality of ends of the connecting pipeline (8), and a pressure port filter (9) is arranged at one of the plurality of ends of the connecting pipeline (8). One of the several ends is connected to a finished product bag (13), one of the several ends of the connecting pipeline (8) is connected to a second connecting portion A (14), one of the several ends of the connecting pipeline (8) is connected to a liquid filter (15), an end of the gas interface filter (402) is connected to a gas interface body (16), an end of the pressure interface filter (9) is connected to a pressure interface body (17), a pipeline fixing block (18) is provided on one side of the support plate (5), and one of the several ends of the connecting pipeline (8) is connected to an air filter (21).

5. A cell culture fluid delivery device according to claim 1, characterized in that: The transfer bag kit (22) comprises a cell transfer bag (2201), a culture medium transfer bag (2202) is arranged on the side of the cell transfer bag (2201), other transfer bags (2203) are arranged on the side of the culture medium transfer bag (2202), a PBS transfer bag (2204) is arranged on the side of the other transfer bag (2203), and the bottom of the cell transfer bag (2201) is connected to a second connecting part B (2205).

6. A cell culture fluid delivery device according to claim 5, characterized in that: The aseptic connector is composed of a first connection part A (403), a first connection part B (10) and a second connection part A (14) and a second connection part B (2205) in a corresponding combination.

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