Combined cell climbing slide

Abstract

The utility model relates to the field of experimental equipment, and provides a combined cell slide which comprises a base and a plurality of slide pieces, the slide pieces are fan-shaped, the plurality of slide pieces are detachably connected with the base, and a circular or fan-shaped cell slide is formed on the base. The problem that it is difficult to ensure the consistency of the scratch width when scratches are manufactured through a manual gun head is solved, meanwhile, the slide piece can be replaced, in this way, the experiment of dynamically observing migrated cells can be repeatedly carried out, and the problem that only cell migration at a fixed time point at the scratch position can be observed on a traditional slide of an overall structure is solved.

Description

Technical Field

[0001] The utility model belongs to the field of experimental equipment, and in particular relates to a combined cell climbing slide. Background Art

[0002] Cell slides are formed by placing a glass slide in a culture medium containing cells. After a certain period of incubation, the cells adhere to the glass slide and grow on it. Cell slides are often needed in in vitro cell experimental studies such as histology, immunohistochemistry, frozen sections, cell smears, in situ hybridization, etc., and are of great significance to the in-depth research of life and medicine.

[0003] When conducting cell co-culture, cell invasion and migration experiments, for example, the cell scratch assay is a laboratory technique that studies cell migration, repair ability and cell-to-cell interaction by scratching a cell monolayer and capturing images regularly through a time-lapse microscope. The basic principle is: artificially create a blank area on the fused monolayer of cells, called a "scratch / wound", and the cells at the edge of the scratch will gradually enter the blank area to heal the "scratch / wound". Images are captured at the beginning and regularly during the cell migration process. By measuring the scratch spacing at different time points and calculating the difference, the cell migration ability can be judged. Because the cell slides currently available on the market are the overall structure of a single circular cell slide, when conducting the above-mentioned cell scratch experiment, it is necessary to scratch the cell slide and then observe the migration of cells to the hollow area of ​​the scratch. First, the scratch artificial error is relatively large, and the scratch position after treatment is fixed. On the traditional overall structure slide, only the cell migration at the scratch position at a fixed time point can be observed. Utility Model Content

[0004] In order to overcome the above-mentioned shortcomings of the prior art, the purpose of the utility model is to provide a combined cell climbing sheet.

[0005] The technical solution adopted by the utility model to solve its technical problems is:

[0006] A combined cell slide, comprising:

[0007] A base and a plurality of climbing pieces;

[0008] The climbing sheet piece is fan-shaped, and a plurality of the climbing sheet pieces are detachably connected to the base, and form a circular or fan-shaped cell climbing sheet on the base.

[0009] Preferably, a connecting rod is provided in the middle of the base, and one end of the climbing piece is detachably connected to the connecting rod.

[0010] Preferably, the small end of the climbing piece is detachably connected to the connecting rod.

[0011] Preferably, a first connecting portion is provided at the top of the connecting rod, and a second connecting portion is provided at the bottom of the small end of the climbing piece, and the first connecting portion and the second connecting portion are detachably connected.

[0012] Preferably, the first connecting portion is a connecting groove, and the second connecting portion is a protruding piece matching with the connecting groove.

[0013] Preferably, the first connecting portion is a first magnet component, the second connecting portion is a second magnet component, and the first magnet component and the second magnet component can be attracted to each other.

[0014] Preferably, a plurality of supporting feet are provided on the bottom end surface of the climbing piece.

[0015] Preferably, one support foot is provided at the small end of the climbing piece, and two support feet are provided at the large end of the climbing piece.

[0016] Preferably, the number of the climbing sheet members is six, and the cell climbing sheet is circular.

[0017] Preferably, the outer periphery of the climbing piece is provided with a wear-resistant portion, the wear-resistant portion is in a U-shaped structure, and the wear-resistant portion in the U-shaped structure extends from the top surface of the climbing piece through the outer periphery of the climbing piece to the bottom surface of the climbing piece.

[0018] Compared with the prior art, the beneficial effects of the utility model include:

[0019] The number of crawling slides in the present application is several, and because the crawling slides can be combined or separated, cells are inoculated on non-adjacent crawling slides, and after the cells are fully grown, blank crawling slides (without inoculated cells) are inserted between non-adjacent crawling slides, which are taken out at intervals and the cells are stained (either crystal violet or DAPI). This allows dynamic observation of the migrating cells and their number, and does not require the use of an artificial gun tip to make scratches, thus overcoming the problem of difficulty in ensuring the consistency of the scratch width when using an artificial gun tip to make scratches. At the same time, the blank crawling slides can be replaced, so that the experiment of dynamically observing the migrating cells can be repeated, overcoming the problem that the traditional crawling slide with an integral structure can only observe the migration of cells at a fixed time point at the scratch position. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0021] Figure 1It is a schematic diagram of the utility model.

[0022] Figure 2 It is a structural diagram of the climbing piece of the utility model.

[0023] Figure 3 It is a schematic diagram of the connection between the first connecting part and the second connecting part of the utility model.

[0024] Figure 4 It is a schematic diagram of the connection between the wear-resistant part and the climbing piece of the utility model.

[0025] Figure 5 This is a schematic diagram showing that the cell climbing sheet of the utility model has a semicircular structure.

[0026] Figure 6 This is a schematic diagram of the operation of the utility model for observing cell migration.

[0027] in:

[0028] 1-base, 2-climbing piece, 3-connecting rod, 4-first connecting part, 5-second connecting part, 6-supporting foot, 7-wear-resistant part. DETAILED DESCRIPTION

[0029] In order to more clearly understand the above-mentioned purposes, features and advantages of the utility model, the utility model is described in detail below in conjunction with the accompanying drawings and specific embodiments. It should be noted that, in the absence of conflict, the embodiments of the present application and the features in the embodiments can be combined with each other. In the following description, many specific details are set forth in order to fully understand the utility model, and the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the utility model.

[0030] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention.

[0031] Example:

[0032] like Figure 1-6 As shown, this embodiment provides a combined cell climbing sheet, including:

[0033] A base 1 and a plurality of climbing pieces 2;

[0034] The climbing sheet member 2 is fan-shaped, and a plurality of climbing sheet members 2 are detachably connected to the base 1 to form a cell climbing sheet on the base 1 .

[0035] The number of the climbing sheet parts 2 of the present embodiment is several, and different numbers of climbing sheet parts 2 are combined and connected with the base 1 to form cell climbing sheets of different sizes, which can be circular, semicircular or fan-shaped cell climbing sheets. For example, there are rectangular or circular culture dishes and well plates in the prior art. Therefore, the culture dishes adapted to the combined cell climbing sheet of the present application can be circular or rectangular structures. When different numbers of climbing sheet parts of the combined cell climbing sheet of the present application are combined and connected with the base, the cell climbing sheets of different sizes formed can also be semicircular, and the shape of the base can also match the shape of the cell climbing sheet. At this time, the overall size of the circular cell climbing sheet is smaller, and a rectangular culture dish or well plate of the corresponding size can be selected, such as Figure 5 , which is a schematic diagram of adapting to a rectangular culture dish, so it can be adapted to different types of culture dishes and well plates.

[0036] At the same time, because the slides can be combined or separated, by inoculating cells on non-adjacent slides, and after the cells are fully grown, insert blank slides (without inoculated cells) between non-adjacent slides, take them out at intervals, and stain the cells (crystal violet or DAPI can be used), you can dynamically observe the migrating cells and their number. For specific operations for observing cell migration, see Figure 6 , place 3 crawling slides (labeled a, b, c) in a plate and inoculate cells. After the cells are fully grown, separate them in order and insert 3 blank crawling slides (labeled d, e, f) at intervals. Take out one of the crawling slides (labeled d, e, f) at regular intervals and stain the cells (either crystal violet or DAPI). Then, the migrated cells and their number can be dynamically observed. The above operation does not require the use of an artificial gun tip to make scratches, thus overcoming the problem that it is difficult to ensure the consistency of the scratch width when making scratches with an artificial gun tip. At the same time, the blank crawling slides can be replaced, so that the experiment of dynamically observing the migrated cells can be repeated, thus overcoming the problem that the traditional crawling slide with an integral structure can only observe the migration of cells at a fixed time point at the scratch position.

[0037] At the same time, because the slides can be combined or separated, different types of cells can be inoculated on different slides, and different types of cells can be separated and recombined together to form cell slides with different characteristics. This type of slide can better control the morphology, growth, migration and other characteristics of cells during cell co-culture, providing more opportunities and possibilities for cell experiments and applications.

[0038] In this embodiment, regarding the connection structure between the climbing piece 2 and the base 1 , specifically, a connecting rod 3 is provided in the middle of the base 1 , and one end of the climbing piece 2 is detachably connected to the connecting rod 3 .

[0039] Specifically, the small end of the climbing piece 2 is detachably connected to the connecting rod 3 .

[0040] Specifically, a first connecting portion 4 is provided at the top of the connecting rod 3, and a second connecting portion 5 is provided at the bottom of the small end of the climbing piece 2. The first connecting portion 4 and the second connecting portion 5 are detachably connected.

[0041] Specifically, the first connecting portion 4 is a connecting groove, and the second connecting portion 5 is a protruding piece matched with the connecting groove.

[0042] Specifically, the first connecting portion 4 is a first magnet component, and the second connecting portion 5 is a second magnet component. The first magnet component and the second magnet component can be attracted to each other.

[0043] In the above connection structure, the connecting rod 3 not only plays a connecting role, but also plays a positioning role because the connecting rod 3 is located at the center of the circular cell climbing sheet, thereby ensuring the positional relationship between the climbing sheet parts 2 and ensuring that a circular cell climbing sheet can be formed.

[0044] In this embodiment, with regard to the structure of the climbing piece 2 , specifically, a plurality of supporting legs 6 are provided on the bottom end surface of the climbing piece 2 .

[0045] Specifically, a foot 6 is provided at the small end of the climbing piece 2 , and two feet 6 are provided at the large end of the climbing piece 2 .

[0046] Specifically, the number of the climbing pieces 2 is six.

[0047] The provided support legs 6 can create a gap between the climbing piece 2 and the base 1 or the culture dish, so that the experimenter can quickly pick up or move it with tweezers, avoiding the difficulty of lifting the climbing piece 2 with tweezers due to the climbing piece 2 relying on the surface tension of the liquid to fit tightly with the base 1 or the bottom of the culture dish. This not only reduces the damage of the tweezers to the climbing piece 2 and the adherent cells, but also helps to improve work efficiency.

[0048] At the same time, a wear-resistant portion 7 is provided on the outer periphery of the climbing piece 2. The wear-resistant portion 7 is in a U-shaped structure, and the wear-resistant portion 7 in the U-shaped structure extends from the top surface of the climbing piece 2 through the outer periphery of the climbing piece 2 to the bottom surface of the climbing piece 2. When the climbing piece 2 is clamped with tweezers, the wear-resistant portion 7 prevents the tweezers from wearing the climbing piece 2; and compared with the climbing piece 2, the wear-resistant portion 7 is less suitable for cell adhesion growth, so the tweezers clamping the wear-resistant portion 7 will not cause damage to the cells.

[0049] The above description is only a preferred embodiment of the present invention and does not constitute any form of limitation to the present invention. Therefore, any modification, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A combined cell slide, characterized in that: include: A base and a plurality of climbing pieces; The climbing sheet is fan-shaped, and a plurality of the climbing sheet are detachably connected to the base, and form a circular or fan-shaped cell climbing sheet on the base; A connecting rod is provided in the middle of the base, and one end of the climbing piece is detachably connected to the connecting rod; The small end of the climbing piece is detachably connected to the connecting rod.

2. The combined cell slide according to claim 1, characterized in that: A first connecting portion is provided at the top of the connecting rod, and a second connecting portion is provided at the bottom of the small end of the climbing piece, and the first connecting portion and the second connecting portion are detachably connected.

3. The combined cell slide according to claim 2, characterized in that: The first connecting portion is a connecting groove, and the second connecting portion is a protruding piece matched with the connecting groove.

4. The combined cell slide according to claim 2, characterized in that: The first connecting portion is a first magnet component, the second connecting portion is a second magnet component, and the first magnet component and the second magnet component can be attracted to each other.

5. The combined cell slide according to claim 1, characterized in that: A plurality of supporting feet are arranged on the bottom end surface of the climbing piece.

6. The combined cell slide according to claim 5, characterized in that: One support foot is provided at the small end of the climbing piece, and two support feet are provided at the large end of the climbing piece.

7. The combined cell slide according to claim 1, characterized in that: The number of the climbing sheet pieces is six, and the cell climbing sheet is circular.

8. The combined cell slide according to claim 1, characterized in that: The outer periphery of the climbing piece is provided with a wear-resistant portion, which is in a U-shaped structure. The wear-resistant portion in the U-shaped structure extends from the top surface of the climbing piece through the outer periphery of the climbing piece to the bottom surface of the climbing piece.

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