Stem cell differentiation culture device

Abstract

The invention relates to the technical field of stem cell culture, and one embodiment of the invention provides a stem cell differentiation culture device which comprises a culture box and further comprises an air inlet pipe, a main fan, a fixed box, a conversion cylinder, a filter cylinder, an auxiliary fan, a discharge box and a cleaning mechanism, the culture box is communicated with a temperature adjusting system through the air inlet pipe, the fixed box is communicated with the air inlet pipe, and the air inlet pipe is communicated with the main fan. The conversion cylinder is rotatably arranged in the fixed box, the interior of the conversion cylinder is divided into two conversion cavities, the filter cylinder is fixedly connected to the interior of the conversion cavities and communicates with the air inlet, the auxiliary fan is installed in the filter cylinder, the discharge box communicates with the fixed box through the discharge port, and the discharge box communicates with a discharge pipe. The cleaning mechanism is used for being matched with the auxiliary fan to clean the inner wall of the filter cartridge. By means of the technical scheme, the technical problem that dust accumulates on a filter screen in a ventilation system in the prior art, and regular disassembly and cleaning are needed is solved.

Description

Technical Field

[0001] Embodiments of the present disclosure relate to the technical field of stem cell culture, and in particular, to a stem cell differentiation culture device. Background Art

[0002] Stem cells are undifferentiated or partially differentiated cells that have the ability to self-renew and differentiate multiple times. Under certain conditions, they can differentiate into multiple functional cells. Since stem cells have the potential to regenerate tissues and repair, they can replace damaged or diseased cells in the body. Therefore, they have broad application prospects in the fields of autoimmune diseases, nervous system diseases, cardiovascular diseases, skeletal system diseases, ophthalmic diseases, and metabolic diseases. At present, cell culture incubators are used to differentiate and culture stem cells. Cell culture incubators can provide a constant temperature, constant humidity, and sterile environment. They are usually made of stainless steel and polished inside to reduce bacterial adhesion. At the same time, cell culture incubators are equipped with independent air circulation systems and CO 2 Control device to keep the culture environment stable and sterile.

[0003] The ventilation system keeps the interior of the incubator in a relatively stable environment. The ventilation system is equipped with filters to filter and block dust and impurities in the environment to avoid pollution to the culture environment. However, as the gas flows and time accumulates, dust will accumulate on the surface of the filter, which will affect the ventilation volume and ventilation efficiency over time. The accumulation of dust and impurities can easily breed bacteria and destroy the environment in the incubator. Therefore, filters need to be cleaned and maintained regularly, which takes up the culture time of stem cells. Summary of the invention

[0004] In order to overcome the above-mentioned defects, the embodiments of the present disclosure provide a stem cell differentiation culture device, which solves the technical problem in the prior art that dust accumulates on the filter screen in the ventilation system and needs to be regularly disassembled and cleaned.

[0005] According to one aspect, at least one embodiment of the present disclosure provides a stem cell differentiation culture device, including a culture box and a box door, and further comprising: An air inlet pipe, through which the incubator is connected to a temperature control system; A main fan, the main fan being installed inside the air inlet duct; A fixed box, the fixed box is fixedly connected to the interior of the incubator, the fixed box is communicated with the air inlet pipe, and an air outlet is provided on the fixed box; A conversion cylinder, the conversion cylinder is rotatably arranged inside the fixed box, the outer wall of the conversion cylinder is tightly attached to the inner wall of the fixed box, the interior of the conversion cylinder is divided into two conversion chambers, and the conversion cylinder is provided with two air inlets matching the air inlet pipe and two bottom openings matching the air outlets; A filter cartridge, wherein two filter cartridges are provided, the filter cartridges and the conversion chambers correspond one to one, the filter cartridges are fixedly connected to the inside of the conversion chamber, the filter cartridges are communicated with the air inlet, the filter cartridges include a barrel and a barrel bottom, the barrel bottom is detachably connected to the bottom of the barrel, a magnet is fixedly connected to the bottom of the barrel, and the barrel bottom is made of iron metal; Auxiliary fan, the auxiliary fan is installed inside the filter cartridge; A discharge box, the discharge box is connected to the fixed box through a discharge port, when the air inlet of one of the conversion cylinders is connected to the air inlet pipe, the bottom opening of another conversion cylinder is connected to the discharge port, and the discharge box is connected to a discharge pipe; A cleaning mechanism, the cleaning mechanism is used to cooperate with the auxiliary fan to clean the inner wall of the filter cartridge, and the cleaning mechanism includes: A cleaning frame, the cleaning frame is rotatably connected to the bottom of the filter cartridge, a brush is connected to the cleaning frame, and the brush contacts the inner wall of the filter cartridge; An opening and closing component, which is used to open and close the bottom of the cylinder, and includes: A lifting plate, the lifting plate can be lifted and lowered inside the discharge box; A pull plate, the pull plate being fixedly connected to the bottom end of the bottom of the cylinder; A grab plate, wherein two grab plates are provided, and the grab plates are movably arranged on the lifting plate, and the two grab plates move relative to each other to grab the pulling plate; A driving assembly, wherein the driving assembly and the opening and closing assembly cooperate to connect the cleaning frame and the auxiliary fan, and the driving assembly comprises: A tooth clutch, the tooth clutch comprises a half clutch one and a half clutch two, the half clutch one is fixedly connected to the auxiliary fan, the half clutch two is connected to the cleaning frame via a buffer spring and a telescopic rod, and the half clutch one is provided with a through hole for the telescopic rod and the buffer spring to pass through.

[0006] In order to further improve the cleaning effect of the filter cartridge, an auxiliary cleaning component is also included, and the auxiliary cleaning component includes: A spray washing pipe, wherein a plurality of the spray washing pipes are provided, a plurality of spray washing slots are provided on the incubator, the spray washing pipes correspond to the spray washing slots one by one, and the spray washing pipes can be raised and lowered and arranged inside the spray washing slots; A delivery pump, wherein the output end of the delivery pump is connected to the spray pipe and is used for delivering cleaning agent to the spray pipe.

[0007] In order to reduce the humidity in the filter cartridge, a drying component is also included. The drying component and the spray washing pipe cooperate to dry the filter cartridge. The drying component includes: A heating box is provided with a heater inside the heating box, and the spray cleaning pipe is connected with the delivery pump and the heating box through a conversion valve.

[0008] In order to further improve the disinfection and sterilization effect in the filter cartridge, an auxiliary sterilization component is also included, and the auxiliary sterilization component includes: A sterilization chamber, the sterilization chamber is connected to the fixed box, the conversion cylinder is provided with two openings, the openings correspond to the conversion chambers one by one, and a transparent window is installed inside the openings; An ultraviolet lamp is installed inside the sterilization chamber.

[0009] In order to concentrate the gas filtered by the filter cartridge, a protective cover is fixedly connected to the interior of the conversion chamber, the protective cover is communicated with the bottom opening, the filter cartridge is located inside the protective cover, and the protective cover is made of transparent material.

[0010] In order to realize the transmission connection between the push rod and the lifting plate, the lifting plate is connected to the push rod through a transmission assembly, and the transmission assembly includes: A driving rack, the driving rack being fixedly connected to the push rod; A driven rack, the driven rack being fixedly connected to the lifting plate; A transmission gear is rotatably connected to the inside of the discharge box, the driving rack and the driven rack are centrally symmetrical with respect to the transmission gear, and both the driving gear and the driven rack are meshed with the transmission gear.

[0011] The beneficial effects of the embodiments of the present disclosure are: 1. In the present disclosure, the main fan and the auxiliary fan are rotated to allow the air inlet pipe to supply air to the interior of the filter cartridge, the temperature of the incoming air is adjusted by the temperature control system, and the incoming air is filtered by the filter cartridge and then sent to the interior of the incubator, so that the gas entering the incubator is constant temperature clean gas.

[0012] 2. In the present disclosure, the two filter cartridges work alternately by rotating the conversion cylinder. While one filter cartridge filters the incoming air, the other filter cartridge is in the cleaning position. The push rod pushes the semi-clutch 2 to rise and cooperate with the semi-clutch 1. The auxiliary fan drives the semi-clutch 2 and the cleaning frame to rotate, driving the brush to clean the inner wall of the filter cartridge. The grab plate clamps the pull plate, and the push rod rises to drive the lifting plate to descend, thereby pulling the bottom of the cartridge downward to open the filter cartridge. The dust and impurities cleaned can be discharged through the discharge box and the discharge pipe.

[0013] 3. In the present disclosure, the delivery pump and the spray pipe are connected through the conversion valve, the cleaning agent is delivered to the spray pipe by the delivery pump, and descends into the interior of the filter cartridge through the spray pipe to flush the inner wall of the filter cartridge. The flushed liquid is discharged through the discharge box and the discharge pipe, and dust and impurities are flushed away at the same time to improve the cleaning effect. The cleaning agent drives the cleaning and sterilization effects, cleans and disinfects the auxiliary fan and the filter cartridge, and ensures the cleanliness of the gas entering the incubator.

[0014] 4. In the present disclosure, ultraviolet rays are irradiated into the interior of the conversion cavity through an ultraviolet lamp and a transparent window to perform auxiliary sterilization and disinfection on the filter cartridge that is not in a working state, thereby further improving the cleaning effect of the filter cartridge and ensuring the cleanliness of the incoming air.

[0015] 5. In the present disclosure, after cleaning is completed, the spray pipe and the heating box are connected through the conversion valve, the air in the heating box is heated by the heater, and the hot air enters the interior of the filter cartridge under the action of the auxiliary fan to dry the filter cartridge and the auxiliary fan. The moisture is discharged through the exhaust box and the exhaust pipe, reducing the influence of the cleaning agent on the subsequent air intake.

[0016] 6. Therefore, compared with the cell culture incubator in the prior art, the present invention makes the two filter cartridges work alternately by rotating the conversion cylinder, and makes the temperature-adjusted gas enter the filter cartridge through the air inlet pipe through the auxiliary fan and enter the interior of the incubator after being filtered by the filter cartridge, so as to ensure the air circulation in the incubator, and drive the tooth clutch to engage by the push rod, so that the auxiliary fan can drive the cleaning frame and the brush to rotate, so as to clean the interior of the filter cartridge, and at the same time, the cleaning agent is sprayed on the interior of the filter cartridge through the cooperation of the delivery pump and the spray pipe, so as to flush and disinfect the filter cartridge and the auxiliary fan, and the filter cartridge is assisted by the ultraviolet lamp to disinfect, and then the heating box and the spray pipe are connected through the conversion valve, and the hot air generated by the heater enters the interior of the filter cartridge under the action of the auxiliary fan, so as to dry the auxiliary fan and the filter cartridge, so as to ensure the cleanliness of the air entering the incubator, and at the same time, avoid the accumulation of dust and impurities in the filter cartridge to affect the air intake, and have the self-cleaning function of the filter cartridge, which reduces the time occupied by disassembly and cleaning for stem cell culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions in the embodiments of the present disclosure, the following briefly introduces the drawings required for use in the description of the embodiments of the present disclosure. Obviously, the drawings described below are only some exemplary embodiments of the present disclosure. For those of ordinary skill in the art, other drawings can be obtained based on the contents of the exemplary embodiments of the present disclosure and these drawings without creative work.

[0018] Figure 1 It is a schematic diagram of the overall first-view structure of an embodiment of the present disclosure; Figure 2 for Figure 1 A schematic diagram of a partially cutaway structure of an embodiment of the present invention; Figure 3 for Figure 1 A schematic diagram of the structure of the air inlet duct, the main fan, the fixed box and the conversion cylinder in the embodiment; Figure 4 for Figure 1 A schematic diagram of the structure of the conversion cylinder, the filter cylinder, the discharge box and the discharge pipe in the embodiment of the invention; Figure 5 for Figure 1 A schematic diagram of the structure of the filter cartridge, auxiliary fan, discharge box and cleaning mechanism in the embodiment of the invention; Figure 6 for Figure 1 A schematic diagram of the structure of the drum bottom, auxiliary fan, cleaning frame and drive assembly in the embodiment; Figure 7 for Figure 1 A schematic diagram of the exploded structure of the drum bottom, auxiliary fan, cleaning frame and driving assembly in the embodiment; Figure 8 for Figure 1 A schematic diagram of the structure of the fixed box, the auxiliary cleaning component and the drying component in the embodiment; Fig. 9 for Figure 1 A schematic diagram of the structure of the fixed box, the discharge box and the heating box in the embodiment of the present invention; Fig.10 for Figure 1 A schematic diagram of the structure of the filter cartridge, the auxiliary cleaning component and the drying component in the embodiment of the invention; Fig.11 for Figure 1 A schematic diagram of the structure of the conversion cylinder, the protective cover and the auxiliary sterilization component in the embodiment of the invention; Fig.12 for Figure 1 A schematic diagram of the overall structure of the second viewing angle in the embodiment of FIG. Fig.13 for Figure 1 A schematic diagram of the structure in which the door is in an open state in the embodiment; Fig.14 for Figure 2 Schematic diagram of the local enlarged structure at point A in the middle.

[0019] In the figure: 1. Incubator; 2. Box door; 3. Air inlet pipe; 4. Main fan; 5. Fixed box; 6. Conversion cylinder; 7. Auxiliary fan; 8. Exhaust box; 9. Exhaust pipe; 10. Motor 1; 11. Protective cover; 101, cylinder body; 102, cylinder bottom; 103, magnet 1; 201. cleaning frame; 202. brush; 301, lifting plate; 302, pulling plate; 303, grabbing plate; 304, electric cylinder 1; 305, electric cylinder 2; 401, half clutch one; 402, half clutch two; 403, fixed plate; 404, telescopic rod; 501, spray washing pipe; 502, delivery pump; 503, electric cylinder three; 601, heating box; 602, heater; 603, switching valve; 701. Sterilization chamber; 702. Ultraviolet lamp. DETAILED DESCRIPTION The present disclosure is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present disclosure, rather than to limit the present disclosure.

[0020] In order to simplify the drawings, only the parts related to the disclosure are schematically shown in each figure, and they do not represent the actual structure of the product. In addition, in order to simplify the drawings and facilitate understanding, in some figures, only one of the parts with the same structure or function is schematically shown, or only one of them is marked. In this article, "one" not only means "only one", but also means "more than one", and "several" includes "two" and "more than two".

[0021] In this document, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in this disclosure can be understood according to specific circumstances.

[0022] In the present disclosure, unless otherwise expressly specified and limited, a first feature being “above” or “below” a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being “above”, “above”, and “above” a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being “below”, “below”, and “below” a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0023] In the description of this embodiment, terms such as "up", "down", "left", and "right" and other orientations or positional relationships are based on the orientations or positional relationships shown in the accompanying drawings and are only for the convenience of description and simplification of operation, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present disclosure.

[0024] In addition, in the description of the present application, the terms "first", "second", etc. are only used to distinguish the description and cannot be understood as indicating or implying relative importance.

[0025] like Figures 1 to 14 As shown, a stem cell differentiation culture device in one embodiment of the present disclosure includes a culture box 1 and a box door 2, and also includes an air inlet pipe 3, a main fan 4, a fixed box 5, a conversion cylinder 6, a filter cylinder, an auxiliary fan 7, an exhaust box 8 and a cleaning mechanism. Compared with the cell culture box 1 in the prior art, the present disclosure rotates the conversion cylinder 6 to make the two filter cylinders work alternately, and the auxiliary fan 7 allows the temperature-controlled gas to enter the filter cylinder through the air inlet pipe and enter the interior of the culture box 1 after being filtered by the filter cylinder, thereby ensuring the air circulation in the culture box 1. The push rod drives the tooth clutch to engage, so that the auxiliary fan 7 can drive the cleaning frame 201 and the brush 202 to rotate, so as to The inside of the filter cartridge is cleaned, and the cleaning agent is sprayed on the inside of the filter cartridge through the cooperation of the delivery pump 502 and the spray pipe 501 to rinse and disinfect the filter cartridge and the auxiliary fan 7. The filter cartridge is assisted in disinfection by the ultraviolet lamp 702, and then the heating box 601 and the spray pipe 501 are connected through the conversion valve 603. The hot air generated by the heater 602 enters the inside of the filter cartridge under the action of the auxiliary fan 7, and dries the auxiliary fan 7 and the filter cartridge to ensure the cleanliness of the air entering the incubator 1. At the same time, dust and impurities are prevented from accumulating in the filter cartridge and affecting the air intake. The filter cartridge has a self-cleaning function, which reduces the time occupied by disassembly and cleaning for stem cell culture.

[0026] The incubator 1 is connected to the temperature control system via the air inlet duct 3. The main fan 4 is installed inside the air inlet duct 3. The air inlet duct 3 is connected to the temperature control system. The temperature control system can use a temperature controller to adjust the temperature of the air entering the air inlet duct 3 to make it consistent with the temperature inside the incubator 1, that is, consistent with the temperature required for stem cell differentiation and culture. The temperature-adjusted air is sent into the interior of the incubator 1 through the main fan 4 to achieve air flow in the incubator 1.

[0027] The fixed box 5 is fixedly connected to the inside of the incubator 1, the fixed box 5 is connected to the air inlet pipe 3, an air outlet is provided on the fixed box 5, a conversion cylinder 6 is rotatably arranged inside the fixed box 5, a motor 10 is installed on the fixed box 5, the conversion cylinder 6 is fixedly connected to the output end of the motor 10, the fixed box 5 and the conversion cylinder 6 are both cylindrical, and the outer wall of the conversion cylinder 6 is tightly attached to the inner wall of the fixed box 5, the interior of the conversion cylinder 6 is divided into two conversion chambers by a partition, the conversion cylinder 6 is coaxially connected to the output end of the motor 10, the conversion cylinder 6 is provided with two air inlets matching the air inlet pipe 3 and two bottom openings matching the air outlet, and the filter cylinder is arranged There are two, the filter cartridge and the conversion chamber correspond one to one, the filter cartridge is fixedly connected to the inside of the conversion chamber, the top inlet of the filter cartridge is connected to the air inlet, the inside of the conversion chamber is fixedly connected with a protective cover 11, the protective cover 11 is connected to the bottom opening, the filter cartridge is located inside the protective cover 11, the protective cover 11 is made of transparent material, the gas filtered by the filter cartridge is protected and concentrated by the protective cover 11, so that the gas enters the inside of the incubator 1 through the bottom opening and the air outlet in time, the filter cartridge includes a barrel 101 and a barrel bottom 102, the barrel bottom 102 is detachably connected to the bottom of the barrel 101, and the bottom of the barrel 101 is fixedly connected with a magnet 103 The material of the bottom 102 of the cylinder is iron metal. The bottom 102 of the cylinder can be conveniently disassembled and assembled through the cooperation of the bottom 102 and the magnet 103. The auxiliary fan 7 is installed inside the filter cylinder. The motor 10 can drive the conversion cylinder 6 to rotate, and the positions of the two conversion chambers are swapped, that is, the positions of the two filter cylinders are switched, so that the two filter cylinders work alternately, and the positions of the air inlet and the air outlet correspond. The filter cylinder rotates to between the air inlet and the air outlet. The main fan 4 and the auxiliary fan 7 cooperate to allow air to enter the interior of the filter cylinder. The dust and impurities in the air can be intercepted through the filter holes on the filter cylinder. At the same time, the filter cylinder can be used Inorganic metal materials, such as silver or copper, have antibacterial and disinfecting effects, and the incubator 1 itself is in a sterile environment to prevent bacteria and viruses from entering the interior of the incubator 1 with the air. After a period of time, the motor 10 drives the conversion cylinder 6 to rotate, and converts the position of the filter cylinder, so that the other filter cylinder rotates to the working position, that is, the position connected to the air inlet pipe 3 and the incubator 1. After working for a period of time, the filter cylinder rotates to a relatively closed position away from the air inlet pipe 3 and the air outlet for cleaning, so as to prevent dust and impurities from accumulating inside the filter cylinder to cause blockage of the filter holes and the generation of bacteria, thereby affecting the air intake effect and the culture effect.

[0028] It should be noted that the motor 10 rotates back and forth to make the conversion chamber rotate 180 degrees at a time to prevent the conversion chamber from continuously rotating and causing the circuit of the auxiliary fan 7 to be entangled or damaged. Then the motor 10 rotates 180 degrees in the opposite direction to restore the conversion chamber to its original position instead of continuously rotating in one direction.

[0029] The discharge box 8 is connected to the fixed box 5 through the discharge port. When the air inlet of one conversion cylinder 6 is connected to the air inlet pipe 3, the bottom opening of the other conversion cylinder 6 is connected to the discharge port. The discharge box 8 is connected to the discharge pipe 9. When one conversion cylinder 6 is in the working position, the other conversion cylinder 6 is in the position corresponding to the discharge box 8. At this time, the interior of the conversion cylinder 6 can be cleaned, and the dust and impurities cleaned can be discharged through the discharge box 8 and the discharge pipe 9 to avoid affecting the stem cells in the incubator 1.

[0030] The cleaning mechanism is used to cooperate with the auxiliary fan 7 to clean the inner wall of the filter cylinder. The cleaning mechanism includes a cleaning frame 201, an opening and closing assembly and a driving assembly. The cleaning frame 201 is rotatably connected to the cylinder bottom 102. The cleaning frame 201 is connected with a brush 202, and the brush 202 contacts the inner wall of the filter cylinder. The opening and closing assembly is used to open and close the cylinder bottom 102. The opening and closing assembly includes a lifting plate 301, a pulling plate 302 and a grabbing plate 303. The lifting plate 301 can be lifted and arranged inside the discharge box 8. An electric cylinder 304 is installed on the discharge box 8. The lifting plate 301 is fixedly connected to the output end of the electric cylinder 304. The pulling plate 302 is fixedly connected to the bottom end of the cylinder bottom 102. Two grabbing plates 303 are provided, and the grabbing plates 303 can be movably arranged on the lifting plate 301. The two grab plates 303 move relative to each other and are used to grab the pulling plate 302. Two electric cylinders 305 are installed on the lifting plate 301. The grab plates 303 and the electric cylinders 305 correspond to each other one by one. The grab plates 303 are fixedly connected to the output end of the electric cylinders 305. When the motor 10 drives the conversion cylinder 6 to rotate the filter cylinder to a position corresponding to the discharge box 8, the pulling plate 302 is located exactly between the two grab plates 303. The grab plates 303 can be driven by the electric cylinder 305 to clamp the pulling plate 302. The grab plates 303 can be driven by the electric cylinder 1 304 to lift and lower. When the grab plates 303 drive the pulling plates 302 to descend, the cylinder bottom 102 and the cleaning frame 201 can be pulled down to open the filter cylinder. The driving assembly and the opening and closing assembly cooperate to adjust the cleaning frame 201 and the auxiliary fan. 7 is connected, the driving assembly includes a tooth clutch, the tooth clutch includes a half clutch 1 401 and a half clutch 2 402, the half clutch 1 401 is fixedly connected to the auxiliary fan 7, a fixing plate 403 is fixedly connected between the outside of the half clutch 1 401 and the inner wall of the auxiliary fan 7, the half clutch 1 401 can be driven to rotate by the auxiliary fan 7, the half clutch 2 402 is connected to the cleaning frame 201 through a buffer spring and a telescopic rod 404, and a through hole for the telescopic rod 404 and the buffer spring to pass through is provided on the half clutch 1 401, when the magnets at the bottom of the cylinder 102 and the bottom of the cylinder body 101 are in close contact, the filter cylinder is in a closed state, the half clutch 2 402 is higher than the half clutch 1 401, the tooth clutch is in a disengaged state, and when the electric cylinder 30 When the pull plate 302, the cylinder bottom 102 and the cleaning frame 201 are driven to descend, the semi-clutch 2 402 descends and engages with the semi-clutch 1 401, and the semi-clutch 1 401 and the semi-clutch 2 402 can be driven to rotate by the auxiliary fan 7, so that the cleaning frame 201 and the brush 202 are driven to rotate by the telescopic rod 404, and the inner wall of the filter cylinder and the inner wall of the cylinder bottom 102 are cleaned. The cleaned dust and impurities fall into the inside of the discharge box 8 through the gap between the cylinder body 101 and the cylinder bottom 102, and are discharged from the incubator 1 through the discharge pipe 9, so as to avoid affecting the differentiation and culture of stem cells in the incubator 1. When the cleaning is completed, the electric cylinder 1 304 drives the lifting plate 301 and the cylinder bottom 102 to rise, so that the cleaning frame 201 drives the semi-clutch 2 402 to rise.The second half clutch 402 and the first half clutch 401 are separated, so that the cleaning frame 201 stops rotating, and the brush 202 is prevented from rotating during the working process, and the dust in the filter cartridge is pushed out through the filter hole.

[0031] In order to further improve the cleaning effect of the filter cartridge, an auxiliary cleaning component is also included, which includes a spray pipe 501 and a delivery pump 502. There are multiple spray pipes 501, and the incubator 1 is provided with multiple spray tanks. The spray pipes 501 correspond to the spray tanks one by one. The spray pipes 501 can be lifted and arranged inside the spray tanks. An electric cylinder 3 503 is installed on the incubator 1. The spray pipe 501 is fixedly connected to the output end of the electric cylinder 3 503 through a lifting frame. The output end of the delivery pump 502 is connected to the spray pipe 501 for delivering cleaning agent to the spray pipe 501. The input end of the delivery pump 502 is connected to the cleaning agent storage tank. During the rotation of the conversion cylinder 6, the spray pipe 501 is inside the spray tank to avoid obstruction to the rotation of the conversion cylinder 6. When the conversion cylinder 6 drives the The filter cartridge rotates to the cleaning position, and the electric cylinder 3 503 drives the spray pipe 501 to descend through the top inlet of the filter cartridge to the inside of the filter cartridge, and the cleaning agent is delivered to the inside of the spray pipe 501 by the delivery pump 502. The cleaning agent is sprayed on the inside of the filter cartridge through the spray pipe 501 to clean the inner wall of the filter cartridge and the auxiliary fan 7. The cleaning agent has the functions of cleaning and sterilizing, and can wash away the dust and impurities on the surface of the filter cartridge and the auxiliary fan 7, and wash them into the discharge box 8 through the gap between the cartridge bottom 102 and the cartridge body 101 and discharge them through the discharge pipe 9. At the same time, the filter cartridge, the auxiliary fan 7 and the inside of the discharge box 8 can be sterilized and disinfected to avoid the accumulation of dust inside the filter cartridge and the discharge box 8 to produce bacteria and viruses, thereby ensuring the normal differentiation and culture of stem cells.

[0032] In order to reduce the humidity in the filter cartridge, a drying component is also included. The drying component and the spray washing pipe 501 cooperate to dry the filter cartridge. The drying component includes a heating box 601. A heater 602 is installed inside the heating box 601. The spray washing pipe 501 is connected to the delivery pump 502 and the heating box 601 through a conversion valve 603. The spray washing pipe 501 can be connected to the delivery pump 502 or the heating box 601 through the conversion valve 603. When it is necessary to clean and disinfect the filter cartridge, the spray washing pipe 501 is connected to the delivery pump 502 through the conversion valve 603, and the cleaning agent is delivered to the inside of the spray washing pipe 501 through the delivery pump 502 to flush the filter cartridge. After flushing, the spray washing pipe 501 is connected to the heating box 601 through the conversion valve 603. Under the action of the auxiliary fan 7, the filter cartridge is placed in a low-pressure environment. At the same time, the heater 602 is used for heating. The heating box 601 is placed in a high-pressure environment, so that the hot air in the heating box 601 enters the interior of the filter cartridge through the spray pipe 501, and heats and dries the filter cartridge and the auxiliary fan 7 to prevent the residual cleaning agent from affecting the subsequent air intake, thereby affecting the humidity in the incubator 1. At the same time, the interior of the spray pipe 501 can be dried to prevent the residual cleaning agent in the spray pipe 501 from entering the interior of the filter cartridge. The interior of the filter cartridge is fixedly connected with a waterproof cover, and the motor in the auxiliary fan 7 is located inside the waterproof cover to prevent the cleaning agent from causing damage to the motor. A fan can be installed inside the heating box 601 to promote the hot air in the heating box 601 to enter the interior of the filter cartridge for drying. The exhaust box 8 and the conversion cylinder 6 are both made of thermal insulation materials to reduce the impact of hot air on the temperature in the incubator 1, thereby ensuring a constant temperature and humidity environment in the incubator 1.

[0033] In order to further improve the disinfection and sterilization effect on the filter cylinder, an auxiliary sterilization component is also included, which includes a sterilization chamber 701 and an ultraviolet lamp 702. The sterilization chamber 701 is connected to the fixed box 5. Two through holes are opened on the conversion cylinder 6. The through holes and the conversion chambers correspond one to one. A transparent window is installed inside the through holes. The ultraviolet lamp 702 is installed inside the sterilization chamber 701. The sterilization chamber 701 is located on the cleaning side of the fixed box 5, that is, the side connected to the discharge box 8. The ultraviolet lamp 702 emits ultraviolet rays to the inside of the conversion chamber located at the cleaning position, and performs auxiliary sterilization and disinfection on the protective cover 11, the filter cylinder and the auxiliary fan 7, so as to further ensure the sterile environment in the incubator 1.

[0034] The working principle and use process of the stem cell differentiation culture device are as follows: The stem cells are placed in the incubator 1, and the temperature of the gas sucked in by the air inlet pipe 3 is regulated by the temperature regulating system. The temperature-regulated gas enters the filter cartridge under the action of the main fan 4 and the auxiliary fan 7, and the dust and impurities in the gas are filtered through the filter cartridge, so that clean air enters the incubator 1; After a period of time, the motor 10 drives the conversion cylinder 6 to rotate, so that the filter cylinder after work rotates to the position connected with the discharge box 8. During the rotation process, the grab plate 303 is at the highest position, and the spray pipe 501 is inside the spray tank. After the rotation is completed, the new filter cylinder is located between the air inlet pipe 3 and the air outlet to filter the intake air. The electric cylinder 2 305 drives the two grab plates 303 to approach each other to clamp the pulling plate 302, and then the electric cylinder 1 304 drives the lifting plate 301 to descend, driving the cylinder bottom 102 and the cleaning frame 201 to descend, so that the half clutch 2 402 and the half clutch 1 401 are engaged, and the tooth clutch and the cleaning frame 201 are driven to rotate through the auxiliary fan 7, so that the brush 202 cleans the inner wall of the filter cylinder; During the cleaning process, the electric cylinder 503 drives the spray pipe 501 to descend to the inside of the filter cartridge at the cleaning position, the spray pipe 501 is connected to the delivery pump 502, the delivery pump 502 delivers the cleaning agent to the inside of the spray pipe 501 and sprays it into the filter cartridge, the cleaning agent flushes the dust and impurities in the filter cartridge into the inside of the discharge box 8, and at the same time disinfects the inside of the filter cartridge and the discharge box 8. After flushing, the spray pipe 501 is connected to the heating box 601 through the conversion valve 603, the heater 602 heats the inside of the heating box 601, and the inside of the heating box 601 is heated. The hot air enters the interior of the filter cartridge through the spray pipe 501 to dry the filter cartridge and the auxiliary fan 7, then the auxiliary fan 7 stops rotating, the heater 602 stops heating, the electric cylinder 1 304 drives the lifting plate 301 and the cartridge bottom 102 to rise, the magnet adsorbs and fixes the cartridge bottom 102, the cleaning frame 201 drives the semi-clutch 2 402 to separate from the semi-clutch 1 401, the electric cylinder 2 305 drives the grab plate 303 away from the pull plate 302, the electric cylinder 3 503 drives the spray pipe 501 to rise to the interior of the spray tank, and prepares for the subsequent conversion and cleaning of the conversion chamber and the filter cartridge.

[0035] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present disclosure rather than to limit it. Although the present disclosure has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present disclosure may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present disclosure, which should be included in the scope of the claims of the present disclosure.

Claims

1. A stem cell differentiation culture device, comprising a culture box (1), characterized in that: Also includes: An air inlet pipe (3), wherein the incubator (1) is connected to the temperature control system via the air inlet pipe (3); A fixed box (5), the fixed box (5) being fixedly connected to the interior of the incubator (1), the fixed box (5) being in communication with the air inlet pipe (3), and an air outlet being provided on the fixed box (5); a conversion cylinder (6), the conversion cylinder (6) being rotatably arranged inside the fixed box (5), the interior of the conversion cylinder (6) being divided into two conversion chambers, the conversion cylinder (6) being provided with two air inlets matching the air inlet pipe (3) and two bottom openings matching the air outlets; A filter cartridge, the filter cartridge is fixedly connected to the interior of the conversion chamber, and the filter cartridge is communicated with the air inlet; An auxiliary fan (7), the auxiliary fan (7) being installed inside the filter cartridge; a discharge box (8), the discharge box (8) being connected to the fixed box (5) via a discharge port, and when the air inlet of one of the conversion cylinders (6) is connected to the air inlet pipe (3), the bottom opening of the other conversion cylinder (6) is connected to the discharge port, and the discharge box (8) is connected to a discharge pipe (9); A cleaning mechanism, the cleaning mechanism is used to cooperate with the auxiliary fan (7) to clean the inner wall of the filter cartridge.

2. A stem cell differentiation culture device according to claim 1, characterized in that: The filter cartridge comprises a cartridge body (101) and a cartridge bottom (102), wherein the cartridge bottom (102) is detachably connected to the bottom of the cartridge body (101).

3. A stem cell differentiation and culture device according to claim 2, characterized in that: A magnet 1 (103) is fixedly connected to the bottom of the cylinder (101), and the cylinder bottom (102) is made of iron metal.

4. A stem cell differentiation culture device according to claim 3, characterized in that: The cleaning mechanism comprises: A cleaning frame (201), the cleaning frame (201) being rotatably connected to the cartridge bottom (102), the cleaning frame (201) being connected to a brush (202), the brush (202) being in contact with the inner wall of the filter cartridge; An opening and closing component, the opening and closing component is used to open and close the cylinder bottom (102); A driving assembly, wherein the driving assembly cooperates with the opening and closing assembly to connect the cleaning frame (201) and the auxiliary fan (7).

5. A stem cell differentiation culture device according to claim 4, characterized in that: The opening and closing assembly comprises: A lifting plate (301), the lifting plate (301) being liftable and arranged inside the discharge box (8); A pull plate (302), the pull plate (302) being fixedly connected to the bottom end of the cylinder bottom (102); A gripping plate (303), wherein two gripping plates (303) are provided, and the gripping plates (303) are movably provided on the lifting plate (301), and the two gripping plates (303) move relative to each other to grip the pulling plate (302).

6. The stem cell differentiation culture device according to claim 5, characterized in that: The drive assembly comprises: A tooth clutch, the tooth clutch comprising a half clutch 1 (401) and a half clutch 2 (402), the half clutch 1 (401) being fixedly connected to the auxiliary fan (7), the half clutch 2 (402) being connected to the cleaning frame (201) via a buffer spring and a telescopic rod (404), and the half clutch 1 (401) being provided with a through hole for the telescopic rod (404) and the buffer spring to pass through.

7. The stem cell differentiation culture device according to claim 6, characterized in that: Also included is an auxiliary cleaning component, the auxiliary cleaning component comprising: A spray washing pipe (501), wherein a plurality of the spray washing pipes (501) are provided, and a plurality of spray washing slots are provided on the incubator (1), wherein the spray washing pipes (501) correspond to the spray washing slots one by one, and the spray washing pipes (501) can be raised and lowered and arranged inside the spray washing slots; A delivery pump (502), wherein an output end of the delivery pump (502) is connected to the spray pipe (501) and is used to deliver cleaning agent to the spray pipe (501).

8. The stem cell differentiation culture device according to claim 7, characterized in that: It also includes a drying component, the drying component and the spray-washing pipe (501) cooperate to dry the inside of the filter cartridge, and the drying component includes: A heating box (601) is provided with a heater (602) inside the heating box (601), and the spray cleaning pipe (501) is connected to the delivery pump (502) and the heating box (601) via a conversion valve (603).

9. The stem cell differentiation culture device according to claim 8, characterized in that: It also includes an auxiliary sterilization component, which includes: A sterilization chamber (701), the sterilization chamber (701) being in communication with the fixed box (5), the conversion cylinder (6) being provided with two openings, the openings corresponding to the conversion chambers one by one, and transparent windows being installed inside the openings; An ultraviolet lamp (702), wherein the ultraviolet lamp (702) is installed inside the sterilization chamber (701).

10. The stem cell differentiation culture device according to claim 9, characterized in that: A protective cover (11) is fixedly connected to the interior of the conversion chamber, the protective cover (11) is in communication with the bottom opening, the filter cartridge is located inside the protective cover (11), and the protective cover (11) is made of a transparent material.

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