Plant stem cell nutrition synergist

Abstract

The invention discloses a plant stem cell nutrition synergist, and belongs to the technical field of plant stem cell nutrient solutions. The nutrient synergist can significantly improve the proliferation rate and survival rate of stem cells, promotes differentiation of the stem cells to target cell types, plays an important role in stem cell culture, can significantly improve the culture efficiency of the stem cells, provides comprehensive nutrient support and a suitable growth environment for stem cell culture, and has good application prospects. Proliferation and differentiation of the stem cells are promoted, and further research on the plant stem cells is facilitated.

Description

Technical Field

[0001] This invention relates to the field of plant stem cell nutrient solution technology, specifically a plant stem cell nutrient enhancer. Background Technology

[0002] Plant stem cells are a type of undifferentiated cell located in specific parts of a plant (such as meristems) and possess the potential for self-renewal and differentiation into various plant cell types. Plant stem cells play a crucial role in plant growth, development, and regeneration.

[0003] Research on plant stem cells has significant scientific and applied value. By studying the molecular and physiological mechanisms of plant stem cells, we can reveal the basic principles of plant growth and development. Stem cell technology can improve crop disease resistance, stress resistance, and yield, thus promoting agricultural development. At the same time, it can develop stem cell-based plant regeneration and propagation technologies, improve plant cloning efficiency and propagation speed, promote the development of horticulture and forestry, and restore damaged ecosystems, thus promoting environmental protection and ecological restoration.

[0004] Significant progress has been made in plant stem cell research, with some key molecular mechanisms already revealed. However, many unknowns remain to be explored, including the regulatory networks of stem cells and the impact of the environment on stem cells. Through continued and in-depth research, plant stem cell technology is expected to achieve wider and more in-depth applications in the future.

[0005] The extraction of plant stem cells involves obtaining stem cells from specific parts of a plant (such as meristematic tissue) and culturing them in vitro to achieve their proliferation and differentiation.

[0006] Stem cell culture requires plant stem cell nutrient solutions, which are specialized culture media for culturing and expanding plant stem cells. Their composition is designed to provide comprehensive nutritional support and a suitable growth environment, promoting stem cell proliferation and differentiation. Specific nutrient enhancers can significantly improve stem cell proliferation and survival rates, while also promoting differentiation into target cell types. Optimized combinations of nutrient enhancers can significantly improve stem cell culture efficiency. Future research into the mechanisms of action of these enhancers and their applications in different types of stem cells is expected to advance stem cell technology in regenerative medicine and cell therapy. Summary of the Invention

[0007] The purpose of this invention is to provide a plant stem cell nutrient enhancer to solve the problems mentioned in the background art.

[0008] To achieve the above objectives, the present invention provides the following technical solution:

[0009] 1) A plant stem cell nutrient enhancer, comprising the following components by concentration: Basic components:

[0010] KNO3: 1.80-2.00 g / L;

[0011] NH4NO3: 1.60-1.70 g / L;

[0012] CaCl2·2H2O: 0.40-0.50 g / L;

[0013] MgSO4·7H2O: 0.35-0.40 g / L;

[0014] KH2PO4: 0.15-0.20 g / L;

[0015] FeSO4·7H2O: 27.50-28.00mg / L;

[0016] Na2EDTA·2H2O: 37.00-37.50mg / L;

[0017] H3BO3: 6.00-6.40 mg / L;

[0018] MnSO4·4H2O: 22.0-22.5mg / L;

[0019] ZnSO4·7H2O: 8.40-8.80mg / L;

[0020] KI: 0.80-0.85 mg / L;

[0021] Na2MoO4·2H2O: 0.20-0.30mg / L;

[0022] CuSO4·5H2O: 0.020-0.030mg / L;

[0023] CoCl2·6H2O: 0.020-0.030mg / L;

[0024] 2) Organic components:

[0025] Glycine: 1.00-3.00 mg / L;

[0026] Niacin: 0.40-0.60 mg / L;

[0027] Thiamine HCl: 0.05-0.15 mg / L;

[0028] Pyridoxine HCl: 0.40-0.60 mg / L;

[0029] Inositol: 90.00-110.00 mg / L;

[0030] 3) Plant hormones:

[0031] 6-Benzylaminopurine (BA): 0.50-1.50 mg / L;

[0032] Indolebutyric acid (IBA): 0.05-0.15 mg / L;

[0033] 4) Carbon source:

[0034] Sucrose: 25.00-35.00g / L.

[0035] As a further preferred embodiment of the present invention: the synergist, calculated by concentration, comprises the following components:

[0036] 1) Basic ingredients:

[0037] KNO3: 1.90 g / L;

[0038] NH4NO3: 1.65 g / L;

[0039] CaCl2·2H2O: 0.44 g / L;

[0040] MgSO4·7H2O: 0.37 g / L;

[0041] KH2PO4: 0.17 g / L;

[0042] FeSO4·7H2O: 27.8 mg / L;

[0043] Na2EDTA·2H2O: 7.3 mg / L;

[0044] H3BO3: 6.2 mg / L;

[0045] MnSO4·4H2O: 22.3 mg / L;

[0046] ZnSO4·7H2O: 8.6 mg / L;

[0047] KI: 0.83 mg / L;

[0048] Na2MoO4·2H2O: 0.25 mg / L;

[0049] CuSO4·5H2O: 0.025 mg / L;

[0050] CoCl2·6H2O: 0.025 mg / L;

[0051] 2) Organic components:

[0052] Glycine: 2.00 mg / L;

[0053] Niacin: 0.50 mg / L;

[0054] Thiamine HCl: 0.10 mg / L;

[0055] Pyridoxine HCl: 0.50 mg / L;

[0056] Inositol: 100.00 mg / L;

[0057] 3) Plant hormones:

[0058] 6-Benzylaminopurine (BA): 1.00 mg / L;

[0059] Indolebutyric acid (IBA): 0.10 mg / L;

[0060] 4) Carbon source:

[0061] Sucrose: 30.00g / L.

[0062] As a further preferred embodiment of the present invention: the synergist, calculated by concentration, comprises the following components:

[0063] 1) Basic ingredients:

[0064] KNO3: 1.80 g / L;

[0065] NH4NO3: 1.60 g / L;

[0066] CaCl2·2H2O: 0.40 g / L;

[0067] MgSO4·7H2O: 0.35 g / L;

[0068] KH2PO4: 0.15 g / L;

[0069] FeSO4·7H2O: 27.50 mg / L;

[0070] Na2EDTA·2H2O: 37.00 mg / L;

[0071] H3BO3: 6.00 mg / L;

[0072] MnSO4·4H2O: 22.0 mg / L;

[0073] ZnSO4·7H2O: 8.40 mg / L;

[0074] KI: 0.80 mg / L;

[0075] Na2MoO4·2H2O: 0.20 mg / L;

[0076] CuSO4·5H2O: 0.020 mg / L;

[0077] CoCl2·6H2O: 0.020 mg / L;

[0078] 2) Organic components:

[0079] Glycine: 1.00 mg / L;

[0080] Niacin: 0.40 mg / L;

[0081] Thiamine HCl: 0.05 mg / L;

[0082] Pyridoxine HCl: 0.40 mg / L;

[0083] Inositol: 90.00 mg / L;

[0084] 3) Plant hormones:

[0085] 6-Benzylaminopurine (BA): 0.50 mg / L;

[0086] Indolebutyric acid (IBA): 0.05 mg / L;

[0087] 4) Carbon source:

[0088] Sucrose: 25.00g / L.

[0089] As a further preferred embodiment of the present invention: it comprises the following components, calculated by concentration:

[0090] 1) Basic ingredients:

[0091] KNO3: 2.00 g / L;

[0092] NH4NO3: 1.70 g / L;

[0093] CaCl2·2H2O: 0.50 g / L;

[0094] MgSO4·7H2O: 0.40 g / L;

[0095] KH2PO4: 0.20 g / L;

[0096] FeSO4·7H2O: 28.00 mg / L;

[0097] Na2EDTA·2H2O: 37.50 mg / L;

[0098] H3BO3: 6.40 mg / L;

[0099] MnSO4·4H2O: 22.5 mg / L;

[0100] ZnSO4·7H2O: 8.80 mg / L;

[0101] KI: 0.85 mg / L;

[0102] Na2MoO4·2H2O: 0.30 mg / L;

[0103] CuSO4·5H2O: 0.030 mg / L;

[0104] CoCl2·6H2O: 0.030 mg / L;

[0105] 2) Organic components:

[0106] Glycine: 3.00 mg / L;

[0107] Niacin: 0.60 mg / L;

[0108] Thiamine HCl: 0.15 mg / L;

[0109] Pyridoxine HCl: 0.60 mg / L;

[0110] Inositol: 110.00 mg / L;

[0111] 3) Plant hormones:

[0112] 6-Benzylaminopurine (BA): 1.50 mg / L;

[0113] Indolebutyric acid (IBA): 0.15 mg / L;

[0114] 4) Carbon source:

[0115] Sucrose: 35.00g / L.

[0116] Compared with the prior art, the beneficial effects of the present invention are:

[0117] The nutrient enhancer in this invention can significantly improve the proliferation rate and survival rate of stem cells, while promoting the differentiation of stem cells into target cell types. It plays an important role in stem cell culture, significantly improving the culture efficiency of stem cells, providing comprehensive nutritional support and a suitable growth environment for stem cell culture, promoting the proliferation and differentiation of stem cells, and facilitating further research on plant stem cells. Detailed Implementation

[0118] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0119] Example 1

[0120] This embodiment provides a plant stem cell nutrient enhancer, which, based on concentration, contains the following components:

[0121] 1) Basic ingredients:

[0122] KNO3: 1.90 g / L;

[0123] NH4NO3: 1.65 g / L;

[0124] CaCl2·2H2O: 0.44 g / L;

[0125] MgSO4·7H2O: 0.37 g / L;

[0126] KH2PO4: 0.17 g / L;

[0127] FeSO4·7H2O: 27.8 mg / L;

[0128] Na2EDTA·2H2O: 7.3 mg / L;

[0129] H3BO3: 6.2 mg / L;

[0130] MnSO4·4H2O: 22.3 mg / L;

[0131] ZnSO4·7H2O: 8.6 mg / L;

[0132] KI: 0.83 mg / L;

[0133] Na2MoO4·2H2O: 0.25 mg / L;

[0134] CuSO4·5H2O: 0.025 mg / L;

[0135] CoCl2·6H2O: 0.025 mg / L;

[0136] 2) Organic components:

[0137] Glycine: 2.00 mg / L;

[0138] Niacin: 0.50 mg / L;

[0139] Thiamine HCl: 0.10 mg / L;

[0140] Pyridoxine HCl: 0.50 mg / L;

[0141] Inositol: 100.00 mg / L;

[0142] 3) Plant hormones:

[0143] 6-Benzylaminopurine (BA): 1.00 mg / L;

[0144] Indolebutyric acid (IBA): 0.10 mg / L;

[0145] 4) Carbon source:

[0146] Sucrose: 30.00g / L.

[0147] Example 2

[0148] This embodiment provides a plant stem cell nutrient enhancer, which, based on concentration, contains the following components:

[0149] 1) Basic ingredients:

[0150] KNO3: 1.80 g / L;

[0151] NH4NO3: 1.60 g / L;

[0152] CaCl2·2H2O: 0.40 g / L;

[0153] MgSO4·7H2O: 0.35 g / L;

[0154] KH2PO4: 0.15 g / L;

[0155] FeSO4·7H2O: 27.50 mg / L;

[0156] Na2EDTA·2H2O: 37.00 mg / L;

[0157] H3BO3: 6.00 mg / L;

[0158] MnSO4·4H2O: 22.0 mg / L;

[0159] ZnSO4·7H2O: 8.40 mg / L;

[0160] KI: 0.80 mg / L;

[0161] Na2MoO4·2H2O: 0.20 mg / L;

[0162] CuSO4·5H2O: 0.020 mg / L;

[0163] CoCl2·6H2O: 0.020 mg / L;

[0164] 2) Organic components:

[0165] Glycine: 1.00 mg / L;

[0166] Niacin: 0.40 mg / L;

[0167] Thiamine HCl: 0.05 mg / L;

[0168] Pyridoxine HCl: 0.40 mg / L;

[0169] Inositol: 90.00 mg / L;

[0170] 3) Plant hormones:

[0171] 6-Benzylaminopurine (BA): 0.50 mg / L;

[0172] Indolebutyric acid (IBA): 0.05 mg / L;

[0173] 4) Carbon source:

[0174] Sucrose: 25.00g / L.

[0175] Example 3

[0176] This embodiment provides a plant stem cell nutrient enhancer, which, based on concentration, contains the following components:

[0177] 1) Basic ingredients:

[0178] KNO3: 2.00 g / L;

[0179] NH4NO3: 1.70 g / L;

[0180] CaCl2·2H2O: 0.50 g / L;

[0181] MgSO4·7H2O: 0.40 g / L;

[0182] KH2PO4: 0.20 g / L;

[0183] FeSO4·7H2O: 28.00 mg / L;

[0184] Na2EDTA·2H2O: 37.50 mg / L;

[0185] H3BO3: 6.40 mg / L;

[0186] MnSO4·4H2O: 22.5 mg / L;

[0187] ZnSO4·7H2O: 8.80 mg / L;

[0188] KI: 0.85 mg / L;

[0189] Na2MoO4·2H2O: 0.30 mg / L;

[0190] CuSO4·5H2O: 0.030 mg / L;

[0191] CoCl2·6H2O: 0.030 mg / L;

[0192] 2) Organic components:

[0193] Glycine: 3.00 mg / L;

[0194] Niacin: 0.60 mg / L;

[0195] Thiamine HCl: 0.15 mg / L;

[0196] Pyridoxine HCl: 0.60 mg / L;

[0197] Inositol: 110.00 mg / L;

[0198] 3) Plant hormones:

[0199] 6-Benzylaminopurine (BA): 1.50 mg / L;

[0200] Indolebutyric acid (IBA): 0.15 mg / L;

[0201] 4) Carbon source:

[0202] Sucrose: 35.00g / L.

[0203] Example 4

[0204] This embodiment provides a method for preparing the above-mentioned plant stem cell nutrient enhancer, including the following steps:

[0205] 1) Weigh each component and dissolve it in distilled water, then add KOH or HCl to the mixed solution to adjust the pH to 5.7-5.8.

[0206] 2) Dispense the prepared solution into culture flasks or petri dishes and cap them;

[0207] 3) Autoclave at 121℃ and 15psi for 20 minutes, then cool after sterilization.

[0208] Example 5

[0209] This embodiment provides a method for using the above-mentioned plant stem cell nutrient enhancer, including the following steps: placing the enhancer in a culture medium, inoculating the plant stem cells into the culture medium, and then culturing them.

[0210] Based on Example 1, an experiment was conducted on spraying this synergist on wheat in Lankao County:

[0211] 1. Test conditions:

[0212] 1.1 Selection of experimental crops and varieties:

[0213] Crop: wheat;

[0214] Variety: Pure Wheat No. 2

[0215] 1.2 Cultivation and Environmental Conditions:

[0216] The experimental demonstration site was selected in Guozhuang Village, Hongmiao Town, Lankao County, Henan Province. The soil is mainly sandy loam with medium to high fertility. The demonstration field is flat, with convenient irrigation and drainage, and easy access, making it convenient for surveys and demonstration visits.

[0217] Wheat was sown on October 21, 2020, at a rate of 15 kg / mu. This was an experimental field and not open to the public. The weather on the day of sowing was sunny with a level 1-2 easterly wind. The highest temperature was 26℃ and the lowest was 6℃. Meteorological data was normal and consistent with wheat sowing conditions.

[0218] 2. Dosage and treatment number of plant stem cell nutrient enhancers used in various experimental settings:

[0219] Processing Number Experimental products Dosage Community area Process 1 All Things Plant Stem Cell Fertilizer 165ml / mu <![CDATA[150m 2 ]]> Process 2 Clear water Spray with an equal amount of water <![CDATA[150m 2 ]]> Process 3 Blank control / <![CDATA[150m 2 ]]>

[0220] Table 1 Processing Design

[0221] Process 1 Process 2 Process 3 Process 2 Process 3 Process 1 Process 3 Process 1 Process 2

[0222] Table 2. Schematic diagram of overlapping locations in each community.

[0223] Isolation rows are set up between residential areas, and protection rows are set up outside the residential areas, with each residential area clearly marked. Routine fertilization is carried out according to local fertilizer application rates and methods, and other management practices are the same for all residential areas.

[0224] 3. Demonstration Treatment and Method Design

[0225] The demonstration adopts a regional design with two processes and no duplication.

[0226] Demonstration field: Apply conventional fertilizer + 165ml / mu of synergistic fertilizer mixed with 50L of water, and spray evenly on the leaves of wheat during the jointing stage, booting stage, grain filling stage and milk stage, for a total of 4 sprays.

[0227] Control field: conventional fertilization + spraying with the same amount of water as the demonstration field at the same time.

[0228] The demonstration field covers an area of ​​50 mu, and the control field covers an area of ​​5 mu.

[0229] 4. Spraying method:

[0230] (1) Spraying time

[0231] Apply the spray evenly to the leaves of wheat during the jointing stage (March 12, 2021), booting stage (April 8, 2021), grain-filling stage (May 12, 2021), and milk stage (May 24, 2021), for a total of 4 sprays.

[0232] Spraying should be done on cloudy or sunny mornings when there is no dew, or after 4:00 PM, avoiding midday high temperatures and strong sunlight to prevent liquid fertilizer evaporation. Do not spray on windy days. The amount of fertilizer solution should be such that it is just about to drip from the leaves, and the soil surface is slightly moist.

[0233] (2) Spraying method

[0234] For manual spraying, use a constant flow rate of 165ml of Wanwu Sheng plant stem cell fertilizer mixed with 15L of river or well water per acre. Maintain uniform spraying, ensuring consistent application rates across replicates of the same treatment. All other agricultural operations are the same for each treatment. Before spraying, thoroughly clean the sprayer and avoid mixing it with other pesticides or fertilizers.

[0235] When using drones for spraying, use 165ml of Wanwusheng plant stem cell fertilizer per acre, mixed with 3L of river or well water.

[0236] Field management is carried out according to local conventional methods, with no difference in management between different plots.

[0237] 5. Agronomic Indicators Survey

[0238] The plant spacing was investigated, and samples were taken from each treatment at each growth stage to investigate plant height, number of tillers, number of leaves, etc.

[0239] Table 3. Agronomic Indicators Survey Record Sheet

[0240]

[0241] 6. Resistance survey

[0242] During wheat growth, pay attention to observing the plant's resistance to stress and disease, and investigate the presence of white spikes in the later stages of growth. The white spike investigation adopts a diagonal 5-point sampling method, with 30 consecutive plants taken at each point, and 150 plants taken for each treatment.

[0243]

[0244] 7. Production Survey

[0245] Before wheat harvest, a five-point diagonal sampling method was used. Each point was surveyed in a double row of 1 meter. The number of ears per mu was calculated. Five ears were randomly selected from each point and brought back indoors to investigate the number of grains per ear. After being fully dried, the weight of 1,000 grains was measured to calculate the yield and yield increase effect.

[0246] Production increase rate calculation:

[0247]

[0248] 8. Results and Analysis

[0249] Table 5. Survey table on the percentage of white ears.

[0250] deal with Total number of samples White ear number White ear rate Increase / decrease compared to control (%) Process 1 150 9 6 -25 Process 2 150 11 7.3 -8.3 Process 3 150 12 8 /

[0251] Table 6 Production Survey Form

[0252]

[0253]

[0254] Analysis: Under the same field management measures, the plants treated with the synergistic fertilizer of this application showed significantly better growth than the water control and blank control areas. The plants were robust with healthy roots; they had more leaves, uniform leaf color, and bright green leaves; the leaves declined later than in the control area, and the photosynthetic time was extended, which is beneficial to the later grain filling. This also indicates that the synergistic agent of this application has a significant effect on soil improvement; the incidence of diseases was relatively mild. The incidence of sheath blight, root rot, stem base rot, and stripe rust in the early stage was less than in other fields, especially in wheat fields sprayed with synergistic fertilizer, where the wheat disease incidence was significantly lower than in wheat fields sprayed with green control agents for integrated pest management; the mid-to-late stage white ear survey showed that the white ear rate in the treatment with synergistic fertilizer (treatment 1) was 6%, which was 1.3 and 2 percentage points lower than the 7.3% and 8% in the water control (treatment 2) and blank control area (treatment 3), respectively.

[0255] The yield results showed that treatment 1 had higher grain number per ear and 1000-grain weight than the other two treatments, indicating that spraying Wanwusheng plant stem cell organic water-soluble fertilizer had a significant effect on promoting grain filling and fullness. The yield results were significant; the treatment (treatment 1) sprayed with Wanwusheng plant stem cell organic water-soluble fertilizer had a yield of 543.5 kg / mu, an increase of 24.1% compared to the control group, demonstrating a significant yield increase.

[0256] In summary, this application of the synergistic fertilizer demonstrates that it has a significant effect on promoting robust wheat plants, enhancing disease resistance, preventing lodging, improving soil, promoting grain filling, filling grains, and increasing yield, and can significantly improve both yield and quality.

[0257] It should be noted that the above embodiments are only specific and clear descriptions of the technical solutions and features of this application. Solutions or features that are prior art or common knowledge to those skilled in the art will not be described in detail in the above embodiments.

[0258] Furthermore, the technical solutions of this application are not limited to the above embodiments. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

Claims

1. A plant stem cell nutrient enhancer, characterized in that, Based on concentration, it contains the following components: Basic ingredients: KNO3: 1.80-2.00 g / L; NH4NO3: 1.60-1.70 g / L; CaCl2·2H2O: 0.40-0.50 g / L; MgSO4·7H2O: 0.35-0.40 g / L; KH2PO4: 0.15-0.20 g / L; FeSO4·7H2O: 27.50-28.00 mg / L; Na2EDTA·2H2O: 37.00-37.50 mg / L; H3BO3: 6.00-6.40 mg / L; MnSO4·4H2O: 22.0-22.5 mg / L; ZnSO4·7H2O: 8.40-8.80 mg / L; KI: 0.80-0.85 mg / L; Na2MoO4·2H2O: 0.20-0.30 mg / L; CuSO4·5H2O: 0.020-0.030 mg / L; CoCl2·6H2O: 0.020-0.030 mg / L; Organic ingredients: Glycine: 1.00-3.00 mg / L; Niacin: 0.40-0.60 mg / L; Thiamine HCl: 0.05-0.15 mg / L; Pyridoxine HCl: 0.40-0.60 mg / L; Inositol: 90.00-110.00 mg / L; Plant hormones: 6-Benzylaminopurine (BA): 0.50-1.50 mg / L; Indolebutyric acid (IBA): 0.05-0.15 mg / L; Carbon source: Sucrose: 25.00-35.00 g / L.

2. The plant stem cell nutrient enhancer according to claim 1, characterized in that, Based on concentration, it contains the following components: 1) Basic ingredients: KNO3: 1.90 g / L; NH4NO3: 1.65 g / L; CaCl2·2H2O: 0.44 g / L; MgSO4·7H2O: 0.37 g / L; KH2PO4: 0.17 g / L; FeSO4·7H2O: 27.8 mg / L; Na2EDTA·2H2O: 7.3 mg / L; H3BO3: 6.2 mg / L; MnSO4·4H2O: 22.3 mg / L; ZnSO4·7H2O: 8.6 mg / L; KI: 0.83 mg / L; Na2MoO4·2H2O: 0.25 mg / L; CuSO4·5H2O: 0.025 mg / L; CoCl2·6H2O: 0.025 mg / L; 2) Organic components: Glycine: 2.00 mg / L; Niacin: 0.50 mg / L; Thiamine HCl: 0.10 mg / L; Pyridoxine HCl: 0.50 mg / L; Inositol: 100.00 mg / L; 3) Plant hormones: 6-Benzylaminopurine (BA): 1.00 mg / L; Indolebutyric acid (IBA): 0.10 mg / L; 4) Carbon source: Sucrose: 30.00 g / L.

3. The plant stem cell nutrient enhancer according to claim 1, characterized in that, Based on concentration, it contains the following components: 1) Basic ingredients: KNO3: 1.80 g / L; NH4NO3: 1.60 g / L; CaCl2·2H2O: 0.40 g / L; MgSO4·7H2O: 0.35 g / L; KH2PO4: 0.15 g / L; FeSO4·7H2O: 27.50 mg / L; Na2EDTA·2H2O: 37.00 mg / L; H3BO3: 6.00 mg / L; MnSO4·4H2O: 22.0 mg / L; ZnSO4·7H2O: 8.40 mg / L; KI: 0.80 mg / L; Na2MoO4·2H2O: 0.20 mg / L; CuSO4·5H2O: 0.020 mg / L; CoCl2·6H2O: 0.020 mg / L; 2) Organic components: Glycine: 1.00 mg / L; Niacin: 0.40 mg / L; Thiamine HCl: 0.05 mg / L; Pyridoxine HCl: 0.40 mg / L; Inositol: 90.00 mg / L; 3) Plant hormones: 6-Benzylaminopurine (BA): 0.50 mg / L; Indolebutyric acid (IBA): 0.05 mg / L; 4) Carbon source: Sucrose: 25.00 g / L.

4. The plant stem cell nutrient enhancer according to claim 1, characterized in that, Based on concentration, it contains the following components: 1) Basic ingredients: KNO3: 2.00 g / L; NH4NO3: 1.70 g / L; CaCl2·2H2O: 0.50 g / L; MgSO4·7H2O: 0.40 g / L; KH2PO4: 0.20 g / L; FeSO4·7H2O: 28.00 mg / L; Na2EDTA·2H2O: 37.50 mg / L; H3BO3: 6.40 mg / L; MnSO4·4H2O: 22.5 mg / L; ZnSO4·7H2O: 8.80 mg / L; KI: 0.85 mg / L; Na2MoO4·2H2O: 0.30 mg / L; CuSO4·5H2O: 0.030 mg / L; CoCl2·6H2O: 0.030 mg / L; 2) Organic components: Glycine: 3.00 mg / L; Niacin: 0.60 mg / L; Thiamine HCl: 0.15 mg / L; Pyridoxine HCl: 0.60 mg / L; Inositol: 110.00 mg / L; 3) Plant hormones: 6-Benzylaminopurine (BA): 1.50 mg / L; Indolebutyric acid (IBA): 0.15 mg / L; 4) Carbon source: Sucrose: 35.00 g / L.

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