Disposable cell culture pore plate

Abstract

The utility model discloses a disposable cell culture pore plate which comprises a culture pore plate body, the culture pore plate body is provided with a plurality of culture pores, the edge of the culture pore plate body is provided with an edge water tank, the culture pore plate body comprises a culture pore plate main body, and the culture pore plate main body is provided with a baffle on the outer side of the edge water tank. According to the utility model, the edge water tank for preventing liquid from evaporating and the anti-backflow baffle are designed, so that the liquid in the edge sealing tank is prevented from shaking and entering the culture holes due to jolting and shaking; the edge water tank can obviously reduce the volatilization of the culture liquid, so that the marginal evaporation effect of the outer culture holes is effectively reduced; the outermost culture pores of the cell culture pore plate (such as a 96-pore plate and a 384-pore plate) can be effectively reused, so that the use cost of the culture plate is greatly reduced; in addition, due to the design of the anti-backflow baffle, the stability of the cell culture pore plate can be supported.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture well plates, in particular to a disposable cell culture well plate. Background Art

[0002] Cell culture well plates are a kind of biological tools with good transparency, non-toxic and sterile, and are widely used in cell culture. Whether it is adherent cells or suspended cells, primary cells or passaged cells, cell culture well plates are used for culture. Cell culture well plates are widely used in cell engineering, biopharmaceuticals, antibody screening, toxicology testing and other fields at home and abroad. The number of culture wells of cell culture well plates currently used is generally 6, 12, 24, 48, 96 and 384. Under the actual cell culture conditions of 37.0 degrees, even if there is a sterile water tank to increase humidity in the incubator, because the outer culture wells are closer to the edge, the cell culture fluid will volatilize a lot due to the edge effect, which often makes the experimental results of the culture wells close to the outside extremely inaccurate and poorly repeatable, especially the performance of 48, 96, and 384 well plates. Therefore, in actual operation, researchers do not use the outer circle of wells, so that 24 wells in the 48-well plate are not used (50% waste), 36 wells in the 96-well plate are not used (37.5% waste), and 76 wells in the 384-well plate are not used (19.8% waste). In order to reduce the edge evaporation effect, researchers generally add PBS or even serum-containing culture medium to the outermost wells, which also causes a large amount of waste of scientific research reagents. Utility Model Content

[0003] The utility model aims to provide a disposable cell culture well plate to solve the problems in the prior art.

[0004] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a disposable cell culture well plate, comprising a culture well plate body, the culture well plate body is provided with a plurality of culture wells, an edge of the culture well plate body is provided with an edge water groove, the culture well plate body comprises a culture well plate body, and a baffle is provided on the outside of the culture well plate body located on the edge of the edge water groove.

[0005] Preferably, the culture well plate body has gaps between adjacent culture wells.

[0006] Preferably, the culture well plate body is provided with an edge sealing groove above the edge water groove, and the width of the edge sealing groove is greater than the width of the edge water groove.

[0007] Preferably, the width of the edge water groove is 2 mm, and the depth of the edge water groove is 10 mm.

[0008] Preferably, the number of the culture wells is 6, 12, 24, 48, 96 or 384.

[0009] Compared with the prior art, the beneficial effects of the utility model are: an edge water tank is designed to prevent liquid evaporation and an anti-backflow baffle is designed to prevent the liquid in the edge sealing tank from shaking into the culture well due to bumps and shaking; this edge water tank can significantly reduce the volatilization of the culture liquid, thereby effectively reducing the marginal evaporation effect of the outer culture wells; the outermost culture wells of the cell culture well plate (such as 96-well plate, 384-well plate) can be effectively reused, greatly reducing the use cost of the culture plate; in addition, the design of the anti-backflow baffle can also support the stability of the cell culture well plate. BRIEF DESCRIPTION OF THE DRAWINGS

[0010] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0011] Figure 1 It is a structural schematic diagram of the utility model;

[0012] Figure 2 It is a cross-sectional view of AA of the utility model;

[0013] Figure 3 This is a schematic diagram of the structure of the utility model with 96 holes;

[0014] Figure 4 It is a diagram of the experimental results of 96 wells of the utility model.

[0015] In the figure: 1. culture well plate body; 2. culture well; 3. edge water tank; 11. culture well plate body; 12. baffle; 13. edge sealing groove; 14. gap. DETAILED DESCRIPTION

[0016] In order to make the purpose, technical scheme and advantages of the embodiments of the utility model clearer, the technical scheme in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model. Therefore, the following detailed description of the embodiments of the utility model provided in the drawings is not intended to limit the scope of the utility model for which protection is claimed, but merely represents selected embodiments of the utility model.

[0017] See also Figure 1-2In an embodiment of the utility model, a disposable cell culture well plate comprises a culture well plate body 1, wherein the culture well plate body 1 is provided with a plurality of culture wells 2, wherein an edge water trough 3 is provided at the edge of the culture well plate body 1, wherein the culture well plate body 1 comprises a culture well plate body 11, wherein a baffle 12 is provided on the outer side of the edge water trough 3, and wherein the culture well plate body 11 is provided with a gap 14 between adjacent culture wells 2; an edge water trough is designed to prevent liquid evaporation, and an anti-backflow baffle is designed to prevent the liquid in the edge sealing groove from shaking into the culture well due to bumps and shaking; such an edge water trough can significantly reduce the volatilization of the cultured liquid, thereby effectively reducing the marginal evaporation effect of the outer culture wells; the outermost culture wells of the cell culture well plate (such as a 96-well plate and a 384-well plate) can be effectively reused, thereby greatly reducing the use cost of the culture plate; in addition, the design of the anti-backflow baffle can also support the stability of the cell culture well plate.

[0018] The culture well plate body 11 is provided with an edge sealing groove 13 above the edge water groove 3, and the width of the edge sealing groove 13 is greater than the width of the edge water groove 3; the width of the edge water groove 3 is 2 mm, and the depth of the edge water groove 3 is 10 mm.

[0019] The number of the culture wells 2 is 6, 12, 24, 48, 96 or 384.

[0020] like Figure 3 ; Taking the 96-well plate as an example, the water loss-time experiment was carried out on the improved cell culture plate (new plate) and the ordinary culture plate (ordinary plate) at the same time. The day the experiment started was recorded as day 0, and the residual water volume was recorded on the 1st, 3rd and 7th days. When recording the residual liquid volume, the data of the outermost circle was recorded as circle 1, the second outer circle as circle 2, the second inner circle as circle 3, and the innermost circle as circle 4 ( Figure 3 ), and statistical analysis of the residual liquid volume was performed. The initial liquid volume was 100 μL, and 4 mL PBS was added to the edge tank. The results are shown in Figure 4 shown.

[0021] According to the statistical results, it can be clearly seen that after 1 day, for ordinary orifice plates, the water loss rate of circle 1 is 6.9%; especially the water loss rate at the four corners of A1, A12, H1, and H12 is 12.4%. In contrast, the water loss rate of the new orifice plate is: 3.7% for circle 1; 6.1% for the four corners. Compared with ordinary orifice plates, the water loss of the new orifice plate is significantly less. After 3 days, for ordinary orifice plates, the water loss rate of circle 1 is 17.3%; the water loss rate at the four corners reaches 30.8%. Circle 2 also has a certain water loss rate of 6.2%. The water loss rate of circle 1 of the new orifice plate is 6.8%; the water loss rate at the four corners is 11.7%; the water loss rate of circle 2 is 4.5%, which is significantly lower than that of ordinary orifice plates overall. After 7 days, for ordinary orifice plates, the water loss rate of circle 1 is as high as 37.7%; especially the water loss rate at the four corners is as high as 56.5%. Circle 2 also has a certain water loss rate of 19.9%. The water loss rate of the new orifice plate in circle 1 is 14.1%; the water loss rate in the four corners is 25.2%, which is much lower than that of the ordinary orifice plate. The water loss rate in circle 2 is only 7.9%. This shows that the improved cell culture orifice plate with edge water tank can significantly improve the water loss problem in the outer circle. The design of the edge water tank can avoid the outer circle orifice plate from being unusable due to a large amount of water loss, and at the same time, the stability of the cell culture orifice plate is strengthened from the design.

[0022] The working principle of the utility model is as follows: when in use, 3mL of sterile PBS needs to be added into the edge sealing groove, thereby improving the use efficiency of the cell culture well plate, truly utilizing the outer holes of the culture plate, and reducing the use cost of the well plate; an edge water groove is designed to prevent liquid evaporation and an anti-backflow baffle is designed to prevent the liquid in the edge sealing groove from shaking into the culture well due to bumps and shaking; the edge water groove can significantly reduce the volatilization of the cultured liquid, thereby effectively reducing the marginal evaporation effect of the outer culture wells; the outermost culture wells of the cell culture well plate (such as a 96-well plate and a 384-well plate) can be effectively reused, greatly reducing the use cost of the culture plate; in addition, the design of the anti-backflow baffle can also support the stability of the cell culture well plate.

[0023] Finally, it should be noted that the above description is only a preferred embodiment of the utility model and is not intended to limit the utility model. Although the utility model is described in detail with reference to the above embodiments, those skilled in the art can still modify the technical solutions recorded in the above embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.

Claims

1. A disposable cell culture well plate, comprising a culture well plate body (1), characterized in that: The culture well plate body (1) is provided with a plurality of culture wells (2), an edge of the culture well plate body (1) is provided with an edge water groove (3), the culture well plate body (1) comprises a culture well plate body (11), and a baffle (12) is provided on the outer side of the culture well plate body (11) which is located at the edge of the edge water groove (3).

2. A disposable cell culture well plate according to claim 1, characterized in that: The culture well plate body (11) is located between adjacent culture wells (2) and has gaps (14).

3. A disposable cell culture well plate according to claim 1, characterized in that: The culture well plate body (11) is provided with an edge sealing groove (13) located above the edge water groove (3), and the width of the edge sealing groove (13) is greater than the width of the edge water groove (3).

4. A disposable cell culture well plate according to claim 1, characterized in that: The width of the edge water groove (3) is 2 mm, and the depth of the edge water groove (3) is 10 mm.

5. A disposable cell culture well plate according to claim 1, characterized in that: The number of the culture wells (2) is 6, 12, 24, 48, 96 or 384.

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