Stem cell full-automatic incubator with uniform heat conduction structure

Abstract

The utility model discloses a full-automatic stem cell incubator with a uniform heat conduction structure. The full-automatic stem cell incubator comprises a full-automatic incubator body, a uniform heat conduction assembly and a culture dish taking-out assembly, the cell culture device has the beneficial effects that by arranging the uniform heat conduction assembly, water in the water bath box can be uniformly heated, so that the water temperature in the water bath box is uniform, cells in the culture dish can be uniformly cultured through the heat conduction cylinder, the cells are uniformly heated, the success rate of cell culture is improved, and the cell culture efficiency is improved. The water temperature can be monitored in real time through a temperature sensor, when the water temperature is low, an electric heating wire can be automatically controlled through a controller to heat water in the water bath box, so that the constant temperature in the water bath box is kept, and by arranging a culture dish taking-out assembly, culture dishes can be conveniently taken out in sequence after culture is finished.

Description

Technical Field

[0001] The utility model relates to a culture box, in particular to a fully automatic culture box for stem cells with a uniform heat conduction structure, belonging to the technical field of cell culture. Background Art

[0002] Stem cells are a type of multipotent cell with the ability to self-replicate. Under certain conditions, they can differentiate into a variety of functional cells. According to the developmental stage of the stem cells, they are divided into embryonic stem cells and adult stem cells. Stem cells are a type of undifferentiated, immature cell that has the potential to regenerate various tissues, organs and the human body. The medical community calls them "universal cells." Stem cells are cells with the potential to proliferate and differentiate. They have the ability to self-renew and replicate, and can produce highly differentiated functional cells. Therefore, it is often necessary to use a fully automatic incubator to culture stem cells in vitro, and it is particularly important to observe their proliferation and growth.

[0003] For example, the stem cell culture box disclosed in publication number CN214529071U is convenient for removing culture medium. It connects three drawers by sliding in layers through three partitions, so that the culture medium can be stored in different categories, managed and controlled in layers, and the culture medium is prevented from interfering with each other and causing qualitative changes due to storage together. It has certain practical effects, and the temperature in a single drawer is uniformly regulated by a temperature regulating tube and a through groove. A single drawer can be opened and closed frequently without affecting the temperature in other drawers through a cover plate that opens and closes separately on the drawer. However, when this type of incubator is in use, since the temperature regulating tube is located on one side of the incubator, the temperature near the temperature regulating tube inside the incubator will be too high when the temperature is adjusted, which can easily cause the cells in the nearby culture dishes to die due to heat, and this heating method causes uneven heating inside the incubator, which can easily cause cell culture failure. Utility Model Content

[0004] The purpose of the present utility model is to provide a fully automatic stem cell incubator with a uniform heat conduction structure in order to solve at least one of the above technical problems.

[0005] The utility model achieves the above-mentioned purpose through the following technical solutions: a fully automatic stem cell incubator with a uniform heat conduction structure, comprising:

[0006] A fully automatic incubator body, which is used to culture stem cells, has a door hinged on its surface, and a transparent observation glass is fixedly connected to the surface of the door;

[0007] A uniform heat-conducting component, which is used to uniformly heat the cells in the culture dish, is arranged inside the fully automatic incubator body, and includes a water bath fixedly connected to the fully automatic incubator body, a heat-conducting cylinder equidistantly fixedly connected to the water bath, and a heating wire for heating, the heating wire being installed on the inner wall of the water bath near the four corners;

[0008] The culture dish removal component is used to remove the cultured cell culture dish from the heat-conducting cylinder. It is arranged inside the full-automatic incubator body and includes a partition fixedly connected to the inside of the full-automatic incubator body, a screw rotatably connected to the inside of the full-automatic incubator body, and a moving block threadedly sleeved on the surface of the screw. A ball head push rod is fixedly connected to the surface of the moving block, the ball head push rod is slidably connected to the partition, and a ball head push rod is slidably connected to the bottom surface of the heat-conducting cylinder.

[0009] As a further solution of the utility model: a temperature sensor is fixedly installed inside the water bath.

[0010] As a further solution of the utility model: a rotating rod is symmetrically rotatably connected inside the water bath, and stirring blades are fixedly connected to the surface of the rotating rod at equal intervals.

[0011] As a further solution of the utility model: the ends of the two rotating rods extend to the outside of the full-automatic incubator body and are connected to the pulleys arranged on the outside of the full-automatic incubator body, the two pulleys are connected by belt transmission, the surface of the full-automatic incubator body is fixedly connected to an L-shaped support plate, the surface of the L-shaped support plate is fixedly connected to a motor, and the motor is fixedly connected to one of the rotating rods.

[0012] As a further solution of the utility model: one end of the screw rod extends to the outside of the full-automatic incubator body and is fixedly connected to a hand wheel arranged on the outside of the full-automatic incubator body.

[0013] As a further solution of the utility model: the end of the ball head push rod is fixedly connected with a bottom plate, the bottom plate and the heat-conducting tube are slidably connected, and a spring is fixedly connected between the bottom plate and the heat-conducting tube.

[0014] The beneficial effects of the utility model are:

[0015] By setting a uniform heat conduction component, the water in the water bath can be evenly heated, so that the water temperature in the water bath is uniform, so that the cells in the culture dish can be evenly cultured through the heat conduction tube, so that the cells are evenly heated, and the success rate of cell culture is improved. In addition, the water temperature can be monitored in real time through the temperature sensor. When the water temperature is low, the controller can automatically control the electric heating wire to heat the water in the water bath, so as to maintain a constant temperature in the water bath.

[0016] By providing a culture dish removal component, it is convenient to take out the culture dishes one by one after the culture is completed. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 The overall structure of the utility model is shown in FIG. Figure 1 ;

[0018] Figure 2 The overall structure of the utility model is shown in FIG. Figure 2 ;

[0019] Figure 3 This is a schematic diagram of the internal structure of the utility model;

[0020] Figure 4 The schematic diagram of the connection structure of the uniform heat conduction component in the utility model is shown in FIG. Figure 1 ;

[0021] Figure 5 The schematic diagram of the connection structure of the uniform heat conduction component in the utility model is shown in FIG. Figure 2 ;

[0022] Figure 6 It is a schematic diagram of the connection structure of the culture dish removal component in the utility model.

[0023] In the figure: 1. Fully automatic incubator body; 2. Incubator door; 3. Transparent observation glass; 4. Uniform heat conduction component; 41. Water bath; 42. L-shaped support plate; 43. Motor; 44. Rotating rod; 45. Pulley; 46. Belt; 47. Stirring blade; 48. Temperature sensor; 49. Heating wire; 410. Heat conduction tube; 5. Culture dish removal component; 51. Partition; 52. Screw; 53. Moving block; 54. Hand wheel; 55. Ball head push rod; 56. Ball head push rod; 57. Bottom plate; 58. Spring. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model. Embodiment 1

[0025] like Figures 1 to 6 As shown, a fully automatic stem cell incubator with a uniform heat conduction structure includes:

[0026] A fully automatic incubator body 1, which is used to culture stem cells, has a door 2 hinged on its surface, and a transparent observation glass 3 is fixedly connected to the surface of the door 2;

[0027] The uniform heat-conducting component 4 is used to uniformly heat the cells in the culture dish, and is arranged inside the fully automatic incubator body 1, and includes a water bath 41 fixedly connected to the fully automatic incubator body 1, a heat-conducting cylinder 410 fixedly connected to the water bath 41 at equal intervals, and a heating wire 49 for heating, and the heating wire 49 is installed on the inner wall of the water bath 41 near the four corners;

[0028] The culture dish removal component 5 is used to remove the cultured cell culture dish from the heat-conducting tube 410. It is arranged inside the full-automatic incubator body 1, and includes a partition 51 fixedly connected to the inside of the full-automatic incubator body 1, a screw 52 rotatably connected to the inside of the full-automatic incubator body 1, and a moving block 53 threadedly sleeved on the surface of the screw 52. A ball head push rod 55 is fixedly connected to the surface of the moving block 53, and the ball head push rod 55 is slidably connected to the partition 51. The bottom surface of the heat-conducting tube 410 is slidably connected to a ball head push rod 56. Embodiment 2

[0029] like Figures 1 to 6 As shown, in addition to all the technical features of the first embodiment, this embodiment also includes:

[0030] A temperature sensor 48 is fixedly installed inside the water bath 41 to monitor the water temperature in the water bath 41 .

[0031] The interior of the water bath 41 is symmetrically rotatably connected to a rotating rod 44, and the surface of the rotating rod 44 is equidistantly fixedly connected to stirring blades 47. During the heating process, the two rotating rods 44 are rotated, thereby driving the stirring blades 47 to rotate, so that the water in the water bath 41 can be stirred, and the water heating can be made more uniform. Therefore, during the culture process, the water temperature can be evenly heated to the cells in the culture dish through the heat conductive tube 410.

[0032] The ends of the two rotating rods 44 extend to the outside of the full-automatic incubator body 1 and are connected to the pulley 45 arranged on the outside of the full-automatic incubator body 1. The two pulleys 45 are connected by a belt 46. An L-shaped support plate 42 is fixedly connected to the surface of the full-automatic incubator body 1. A motor 43 is fixedly connected to the surface of the L-shaped support plate 42. The motor 43 is fixedly connected to one of the rotating rods 44. During the heating process, the motor 43 is started, and the motor 43 runs to drive one of the rotating rods 44 to rotate. The rotating rod 44 rotates and then drives the rotating rod 44 to rotate synchronously through the cooperation of the belt 46 and the pulley 45, which can then drive the stirring blade 47 to rotate. Embodiment 3

[0033] like Figures 1 to 6 As shown, in addition to all the technical features of the first embodiment, this embodiment also includes:

[0034] One end of the screw rod 52 extends to the outside of the full-automatic incubator body 1 and is fixedly connected to a handwheel 54 arranged on the outside of the full-automatic incubator body 1. After the incubation is completed, the user turns the handwheel 54, and the rotation of the handwheel 54 drives the screw rod 52 to rotate, and the rotation of the screw 52 drives the moving block 53 to move, and the movement of the moving block 53 drives the ball head push rod 55 to follow the movement. When the ball head push rod 55 contacts the ball head push rod 56, the ball head push rod 55 continues to move, and the ball head push rod 55 squeezes the ball head push rod 56 to move upward, so that the culture dish can be pushed out of the heat conductive tube 410, making it convenient to take the culture dish.

[0035] The end of the ball head push rod 56 is fixedly connected to the bottom plate 57, the bottom plate 57 and the heat conductive tube 410 are slidably connected, and a spring 58 is fixedly connected between the bottom plate 57 and the heat conductive tube 410. The ball head push rod 56 moves upward and then can push the bottom plate 57 upward, so that the culture dish can be pushed out.

[0036] Working principle: when in use, open the door 2, and then place the culture dishes containing stem cells in the heat-conducting tube 410 in turn, then inject clean water into the water bath 41, at this time, set the temperature monitoring range of the temperature sensor 48 through the control panel, and then start the heating wire 49 to heat the clean water. During the heating process, start the motor 43, the motor 43 runs and then drives one of the rotating rods 44 to rotate, the rotating rod 44 rotates and then drives the rotating rod 44 to rotate synchronously through the cooperation of the belt 46 and the pulley 45, and then can drive the stirring blade 47 to rotate, so that the water in the water bath 41 can be stirred, and then the water heating can be more uniform, so that during the culture process, the water temperature can be evenly heated to the cells in the culture dish through the heat-conducting tube 410, and when the temperature reaches the temperature monitoring range of the temperature sensor 48, the stirring blade 47 can be driven to rotate. After the control range is reached, the heating wire 49 is controlled to stop heating, and then the cells continue to be cultured. During the culture process, when the water temperature in the water bath 41 is low, the heating wire 49 can be automatically controlled by the controller to heat, so as to ensure that the water bath 41 is in a constant temperature state. After the culture is completed, the user turns the hand wheel 54, the hand wheel 54 rotates and then drives the screw 52 to rotate, the screw 52 rotates and then drives the moving block 53 to move, the moving block 53 moves and then drives the ball push rod 55 to follow the movement, when the ball push rod 55 contacts the ball push rod 56, the ball push rod 55 continues to move, the ball push rod 55 will squeeze the ball push rod 56 to move upward, the ball push rod 56 moves upward and then can push the bottom plate 57 to move upward, so that the culture dish can be pushed out of the heat conductive tube 410, so that the culture dish can be taken out conveniently.

[0037] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-restrictive from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention. Any reference numeral in a claim should not be regarded as limiting the claim to which it relates.

[0038] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A fully automatic stem cell incubator with a uniform heat conduction structure, characterized in that: include A fully automatic incubator body (1) is used for culturing stem cells, and has a door (2) hinged on its surface, and a transparent observation glass (3) is fixedly connected to the surface of the door (2); A uniform heat-conducting component (4) for uniformly heating cells in a culture dish, which is arranged inside the fully automatic incubator body (1), and comprises a water bath (41) fixedly connected to the inside of the fully automatic incubator body (1), a heat-conducting cylinder (410) fixedly connected to the inside of the water bath (41) at equal distances, and a heating wire (49) for heating, wherein the heating wire (49) is installed on the inner wall of the water bath (41) near the four corners; A culture dish removal assembly (5) is used to remove a cultured cell culture dish from the heat-conducting tube (410), and is arranged inside the fully automatic incubator body (1), and comprises a partition (51) fixedly connected to the inside of the fully automatic incubator body (1), a screw (52) rotatably connected to the inside of the fully automatic incubator body (1), and a moving block (53) threadedly sleeved on the surface of the screw (52), a ball head push rod (55) fixedly connected to the surface of the moving block (53), the ball head push rod (55) and the partition (51) are slidably connected, and a ball head push rod (56) is slidably connected to the bottom surface of the heat-conducting tube (410).

2. The fully automatic stem cell incubator with a uniform heat conduction structure according to claim 1, characterized in that: A temperature sensor (48) is fixedly installed inside the water bath (41).

3. The fully automatic stem cell incubator with a uniform heat conduction structure according to claim 1, characterized in that: A rotating rod (44) is symmetrically rotatably connected inside the water bath (41), and stirring blades (47) are equidistantly fixedly connected to the surface of the rotating rod (44).

4. The fully automatic stem cell incubator with a uniform heat conduction structure according to claim 3, characterized in that: The ends of the two rotating rods (44) extend to the outside of the fully automatic incubator body (1) and are connected to a pulley (45) arranged on the outside of the fully automatic incubator body (1); the two pulleys (45) are connected to each other by a belt (46); an L-shaped support plate (42) is fixedly connected to the surface of the fully automatic incubator body (1); a motor (43) is fixedly connected to the surface of the L-shaped support plate (42); and the motor (43) is fixedly connected to one of the rotating rods (44).

5. The fully automatic stem cell incubator with a uniform heat conduction structure according to claim 1, characterized in that: One end of the screw rod (52) extends to the outside of the fully automatic incubator body (1) and is fixedly connected to a hand wheel (54) arranged on the outside of the fully automatic incubator body (1).

6. The fully automatic stem cell incubator with a uniform heat conduction structure according to claim 5, characterized in that: The end of the ball head push rod (56) is fixedly connected to a bottom plate (57), the bottom plate (57) and the heat-conducting tube (410) are slidably connected, and a spring (58) is fixedly connected between the bottom plate (57) and the heat-conducting tube (410).

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