Light-excited chemiluminescence detection device and optical system thereof

Abstract

The utility model relates to an optical system of a light-excited chemiluminescence detection device, which is characterized in that a turntable is provided with a light-emitting hole and a collecting hole along the thickness direction in a penetrating manner, and a light source assembly is provided with a light-emitting end facing a first surface; the photomultiplier assembly is provided with a collecting end facing the first surface; the optical fiber tube is provided with a first end, a second end and a third end, the first end is used for being aligned with the light-emitting hole from the second surface and receiving the excitation light transmitted from the light-emitting hole, and the third end is used for being communicated with the reaction cavity and projecting the excitation light into the reaction cavity; the second end is used for aligning with the collecting hole from the second surface and transmitting signal light generated in the reaction cavity into the collecting hole; the driving assembly is used for driving the turntable to rotate to a light-emitting position, a collecting position and a closing position relative to the light source assembly and the photomultiplier assembly; at the closing position, the light-emitting end and the collecting end are shielded by the turntable; at the light-emitting position, the light-emitting end is aligned with the light-emitting hole and the turntable shields the collecting end; at the collecting position, the collecting end is aligned with the collecting hole, and the turntable shields the light-emitting end.

Description

Technical Field

[0001] The utility model relates to the field of medical technology, in particular to a photoinduced chemiluminescence detection device and an optical system thereof. Background Art

[0002] In chemiluminescent immunoassay and fluorescent immunoassay, the luminescence intensity of the reaction solution of the sample is detected to detect the composition and content of the substance contained in the sample. Since the luminescence of the reaction solution belongs to the category of weak light, it is necessary to ensure that the detection system can accurately detect the luminescence intensity of the sample being tested.

[0003] Photoluminescence technology is a directional immunoassay technology. Compared with the traditional magnetic particle chemiluminescence technology, it reduces the magnetic cleaning process, greatly reduces the length of the entire reaction cycle, and improves efficiency. At the same time, it reduces the development cost and development cycle of the whole machine.

[0004] Microfluidics is a technology that uses microchannels to process or operate small droplets to complete the entire biochemical reaction process. It uses fewer samples, has a fast detection speed, is simple to operate, has multi-functional integration, and is small and easy to carry. The photoluminescence technology based on microfluidics reduces the magnetic cleaning link and further reduces the parts of magnet adsorption, liquid injection, liquid filming, cleaning and other links in the overall design, which can further compress the complexity of the whole machine, reduce the volume, and reduce the weight. This makes the volume and weight of the microfluidic machine more compact, even reaching the stage of handheld equipment.

[0005] However, some traditional photochemiluminescence optical paths usually include an excitation optical path and a collection optical path. The laser optical path is always on during operation, and a shutter-like switch mechanism needs to be separately set up to control the on and off of the laser optical path. The emission light collection optical path also needs to be separately set up with a shutter-like switch mechanism to control the on and off of the collection optical path. It is understandable that if there are multiple laser optical paths and collection optical paths, a shutter-like switch structure needs to be separately set up for each optical path, which makes the overall size of the device larger, is not conducive to miniaturization, and increases the manufacturing cost.

[0006] BACKGROUND OF THE PRESENT INVENTION The above information disclosed is only used to understand the background of the concept of the present invention and may contain information that does not constitute prior art. Utility Model Content

[0007] Based on this, it is necessary to provide a photochemiluminescence detection device and its optical system to address the above problems.

[0008] An optical system of a photoinduced chemiluminescence detection device, comprising:

[0009] A rotating disk, wherein the rotating disk has a first surface and a second surface disposed opposite to each other along its thickness direction, and a light-emitting hole and a collecting hole are disposed through the rotating disk along the thickness direction;

[0010] A light source assembly, wherein the light source assembly has a light emitting end facing the first surface;

[0011] A photomultiplier assembly, wherein the photomultiplier assembly has a collecting end facing the first surface;

[0012] an optical fiber tube, the optical fiber tube having a first end, a second end and a third end, the first end being used to align with the light-emitting hole from the second surface and receive the excitation light transmitted from the light-emitting hole, the third end being used to communicate with the reaction chamber and project the excitation light into the reaction chamber, and the second end being used to align with the collection hole from the second surface and transmit the signal light generated in the reaction chamber to the collection hole;

[0013] A driving assembly, the driving assembly is connected to the rotating disk and is used to drive the rotating disk to rotate relative to the light source assembly and the photomultiplier assembly to a light emitting position, a collecting position and a closed position; and

[0014] At the light-emitting position, the light-emitting end is aligned with the light-emitting hole, and the rotating disk covers the collecting end;

[0015] At the collecting position, the collecting end is aligned with the collecting hole, and the rotating disk covers the light-emitting end;

[0016] In the closed position, both the light emitting end and the collecting end are shielded by the rotating disk.

[0017] The optical system of the above-mentioned light-induced chemiluminescence detection device of the utility model can at least achieve the following beneficial effects: the turntable can be driven by the driving component to rotate to the light-emitting position, at which time the light-emitting end of the light source component is aligned with a light-emitting hole and the excitation light is transmitted from the light-emitting hole to the first end of the optical fiber tube, and the optical fiber tube then projects the excitation light from the third end into the reaction chamber; during the reaction of the reactants in the reaction chamber, the turntable can be driven by the driving component to rotate to the closed position to wait for the reaction to be completed, at which time the light-emitting end of the light source component and the collecting end of the photomultiplier component are both blocked by the turntable; after the reaction is completed, the turntable can be driven by the driving component to rotate to the collecting position, at which time the collecting hole on the turntable is aligned with the collecting end of the photomultiplier component, and the third end of the optical fiber tube can receive the signal light in the reaction chamber, and transmit the signal light from the second end to the collecting end of the photomultiplier component through the collecting hole. After the collection is completed, the turntable is driven by the driving component to rotate to the closed position again, at which time the light-emitting end of the light source component and the collecting end of the photomultiplier component are both blocked by the turntable, waiting for the photomultiplier component to cool down and prepare for the next collection work. It should be noted that the optical system of the photochemiluminescence detection device of the present application only uses one turntable to achieve the on-off control of multiple optical paths such as the light-emitting end of the light source component and the collecting end of the photomultiplier component. There is no need to set a switch structure for controlling the on-off for each optical path as in the traditional solution. The structure is more streamlined, in line with the trend and demand of miniaturization of equipment, and has lower manufacturing costs.

[0018] In one embodiment, the light-emitting hole is embedded with an attenuation sheet, which can weaken the intensity of the excitation light emitted by the light-emitting end of the light source assembly.

[0019] In one embodiment, the number of the light-emitting holes is set to be multiple, and the multiple light-emitting holes are arranged at intervals along the circumference of the turntable, and each of the light-emitting holes is embedded with an attenuation sheet, and the attenuation coefficients of the multiple attenuation sheets are different; when in the light-emitting position, the light-emitting end of the light source assembly is aligned with one of the light-emitting holes. This arrangement of this embodiment means that the attenuation sheets in different light-emitting holes have different weakening capabilities for the intensity of the excitation light emitted by the light-emitting end of the light source assembly, and the light-emitting end of the light source assembly can be flexibly selected to be aligned with one of the light-emitting holes as needed, so as to obtain excitation light of different intensities, and only one light source assembly can be set to avoid inter-unit differences.

[0020] In one embodiment, a light-shielding sleeve is provided on the first surface of the rotating disk, the light-shielding sleeve is arranged around the periphery of the collecting hole and is connected to the collecting hole, and when in the collecting position, the collecting end is aligned with the light-shielding sleeve. The light-shielding sleeve can prevent the influence of stray light.

[0021] In one of the embodiments, the number of the collecting holes is set to be multiple, and the multiple collecting holes are arranged at intervals along the circumference of the rotating disk. When in the collecting position, the collecting end of the photomultiplier assembly is aligned with one of the collecting holes.

[0022] In one of the embodiments, the light emitting hole and the collecting hole are spaced apart and have different rotation radii.

[0023] In one of the embodiments, the minimum distance between the hole wall of the light-emitting hole and the rotation center of the turntable is greater than the maximum distance between the hole wall of the collecting hole and the rotation center of the turntable. It is understandable that if the rotation radius of the rotation stroke of the light-emitting hole is the same as the rotation radius of the rotation stroke of the collecting hole, the collecting hole and the light-emitting end of the light source assembly will be briefly docked during the rotation of the turntable, and the light-emitting hole and the collecting end of the photomultiplier assembly will be briefly docked, which may affect the detection. This embodiment emphasizes that the light-emitting hole and the collecting hole are spaced apart and have different rotation radii, and the minimum distance between the hole wall of the light-emitting hole and the rotation center of the turntable is greater than the maximum distance between the hole wall of the collecting hole and the rotation center of the turntable. Therefore, no matter how the turntable rotates, the collecting hole can at most be aligned with the collecting end connected to the photomultiplier assembly, and the light-emitting hole can at most be aligned with the light-emitting end of the light source assembly, which will not affect the detection. In addition, another advantage of this arrangement is that more light-emitting holes can be set on the rotating stroke where the light-emitting holes are located, and will not be restricted by the collecting holes. Similarly, more collecting holes can be set on the rotating stroke where the collecting holes are located, and will not be restricted by the light-emitting holes.

[0024] In one of the embodiments, the minimum distance between the hole wall of the collecting hole and the rotation center of the turntable is greater than the maximum distance between the hole wall of the light-emitting hole and the rotation center of the turntable. It is understandable that if the rotation radius of the rotation stroke of the light-emitting hole is the same as the rotation radius of the rotation stroke of the collecting hole, the collecting hole and the light-emitting end of the light source assembly will be briefly docked during the rotation of the turntable, and the light-emitting hole and the collecting end of the photomultiplier assembly will be briefly docked, which may affect the detection. This embodiment emphasizes that the light-emitting hole and the collecting hole are spaced apart and have different rotation radii, and the minimum distance between the hole wall of the collecting hole and the rotation center of the turntable is greater than the maximum distance between the hole wall of the light-emitting hole and the rotation center of the turntable. Therefore, no matter how the turntable rotates, the collecting hole can at most be aligned with the collecting end connected to the photomultiplier assembly, and the light-emitting hole can at most be aligned with the light-emitting end of the light source assembly, which will not affect the detection. In addition, another advantage of this arrangement is that more light-emitting holes can be set on the rotating stroke where the light-emitting holes are located, and will not be restricted by the collecting holes. Similarly, more collecting holes can be set on the rotating stroke where the collecting holes are located, and will not be restricted by the light-emitting holes.

[0025] In one embodiment, the light source assembly includes a laser module having the light-emitting end, a lens group connected to the light-emitting end of the laser module, and a first filter connected to the lens group. For example, the first filter only retains the 680nm wavelength excitation monochromatic light required for photoinduced chemiluminescence in the excitation light emitted by the laser module, and filters out light of other wavelengths. It should be noted that this is only for explanation and does not limit the first filter to be set in this way.

[0026] In one embodiment, the photomultiplier assembly includes a photomultiplier body having the collecting end and a second filter disposed at the collecting end. For example, the second filter only retains the monochromatic light emitted at a wavelength of 620 nm required for photoinduced chemiluminescence in the signal light and filters out light of other wavelengths. It should be noted that this is only for explanation and does not limit the second filter to be set in this way.

[0027] In one embodiment, the optical system of the photo-induced chemiluminescence detection device also includes a mounting plate, which is located on the second surface of the turntable, and is provided with a first through hole and a second through hole, the first end of the optical fiber tube is fixed to the mounting plate and aligned with the first through hole, and the second end of the optical fiber tube is fixed to the mounting plate and aligned with the second through hole; when in the light-emitting position, the first through hole is aligned with one of the light-emitting holes, and the second through hole is blocked by the turntable; when in the collecting position, the second through hole is aligned with one of the collecting holes, and the first through hole is blocked by the turntable; when in the closed position, both the first through hole and the second through hole are blocked by the turntable.

[0028] In one embodiment, the driving assembly further includes a driving motor and a driving shaft connected to the driving motor, and one end of the driving shaft is fixed to the center of the turntable and is used to drive the turntable to rotate.

[0029] The present application also provides a photoinduced chemiluminescence detection device, which includes a reaction chamber and an optical system as described in any of the above embodiments.

[0030] The above-mentioned photochemiluminescence detection device includes the optical system described in any of the above-mentioned embodiments, so the above-mentioned photochemiluminescence detection device also includes at least the following beneficial effects: the turntable can be rotated to the light-emitting position under the drive of the driving component, at which time the light-emitting end of the light source component is aligned with a light-emitting hole and transmits the excitation light from the light-emitting hole to the first end of the optical fiber tube, and the optical fiber tube then projects the excitation light from the third end into the reaction chamber; during the reaction of the reactants in the reaction chamber, the turntable can be rotated to the closed position under the drive of the driving component to wait for the reaction to be completed, at which time the light-emitting end of the light source component and the collecting end of the photomultiplier component are both blocked by the turntable; after the reaction is completed, the turntable can be rotated to the collecting position under the drive of the driving component, at which time the collecting hole on the turntable is aligned with the collecting end of the photomultiplier component, and the third end of the optical fiber tube can receive the signal light in the reaction chamber, and transmit the signal light from the second end through the collecting hole to the collecting end of the photomultiplier component. After the collection is completed, the turntable is driven by the driving component to rotate to the closed position again. At this time, the light-emitting end of the light source component and the collecting end of the photomultiplier component are both blocked by the turntable, waiting for the photomultiplier component to cool down and prepare for the next collection. It should be noted that the optical system of the photo-induced chemiluminescence detection device of the present application only uses one turntable to realize the on-off control of multiple light paths such as the light-emitting end of the light source component and the collecting end of the photomultiplier component. There is no need to set a switch structure for controlling the on-off for each light path as in the traditional solution. The structure is more streamlined, which meets the trend and demand of miniaturization of equipment and has lower manufacturing costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the conventional technology, the drawings required for use in the embodiments or the conventional technology descriptions are briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0032] Figure 1 A schematic structural diagram of an optical system of a photochemiluminescence detection device provided in one embodiment of the utility model.

[0033] Figure 2 A three-dimensional cross-sectional view of the optical system of a photochemiluminescence detection device provided in one embodiment of the utility model.

[0034] Figure 3 An exploded schematic diagram of a tube body of an optical system of a photo-induced chemiluminescence detection device provided in one embodiment of the utility model.

[0035] Figure 4 A schematic structural diagram of a turntable of an optical system of a photochemiluminescence detection device provided in one embodiment of the utility model.

[0036] Figure 5 An exploded schematic diagram of a light source assembly of an optical system of a photochemiluminescence detection device provided in one embodiment of the utility model.

[0037] Figure 6 An exploded schematic diagram of a photomultiplier component of an optical system of a photoinduced chemiluminescence detection device provided in one embodiment of the utility model.

[0038] Figure 7 A perspective schematic diagram of a portion of a turntable of an optical system of a photochemiluminescence detection device provided in an embodiment of the utility model when the turntable is in a closed position.

[0039] Figure 8 A partial perspective schematic diagram of a turntable of an optical system of a photochemiluminescence detection device provided in one embodiment of the utility model when the turntable is in the light-emitting position.

[0040] Fig. 9 A partial perspective schematic diagram of a turntable of an optical system of a photochemiluminescence detection device provided in one embodiment of the utility model when the turntable is in the collection position.

[0041] Fig.10 A schematic structural diagram of an optical fiber tube provided in one embodiment of the utility model.

[0042] Reference numerals:

[0043] 100, turntable; 110, first surface; 120, second surface; 131, light-emitting hole; 132, collecting hole; 200, light source assembly; 210, light source module; 211, light-emitting end; 220, lens group; 231, first filter; 300, photomultiplier assembly; 310, photomultiplier body; 311, collecting end; 322, second filter; 400, optical fiber tube; 410, first end; 420, second end; 430, third end; 500, drive assembly; 510, drive motor; 520, drive shaft; 600, attenuation plate; 700, light-shielding sleeve; 800, mounting plate; 810, first through hole; 820, second through hole. DETAILED DESCRIPTION

[0044] In order to make the above-mentioned purposes, features and advantages of the utility model more obvious and easy to understand, the specific implementation methods of the utility model are described in detail below in conjunction with the accompanying drawings. In the following description, many specific details are set forth to facilitate a full understanding of the utility model. However, the utility model can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the utility model, so the utility model is not limited by the specific embodiments disclosed below.

[0045] See also Figures 1 to 10 In some embodiments, the utility model provides an optical system of a photochemiluminescence detection device, which includes a turntable 100, a light source assembly 200, a photomultiplier assembly 300, an optical fiber tube 400 and a drive assembly 500. Figures 1 to 4 As shown, the rotating disk 100 has a first surface 110 and a second surface 120 disposed opposite to each other along its thickness direction, and a light emitting hole 131 and a collecting hole 132 are disposed through the rotating disk 100 along the thickness direction; Figure 3 As shown, the light source assembly 200 has a light emitting end 211 facing the first surface 110; Figure 3 As shown, the photomultiplier assembly 300 may be a photomultiplier tube, and the photomultiplier assembly 300 has a collecting end 311 facing the first surface 110; Fig.10 As shown, the optical fiber tube 400 has a first end 410, a second end 420 and a third end 430, the first end 410 is used to align the light-emitting hole 131 from the second surface 120 and receive the excitation light transmitted from the light-emitting hole 131, the third end 430 is used to communicate with the reaction chamber and project the excitation light into the reaction chamber (not shown), and the second end 420 is used to align the collecting hole 132 from the second surface 120 and transmit the signal light generated in the reaction chamber to the collecting hole 132; the driving assembly 500 is connected to the turntable 100 and is used to drive the turntable 100 to rotate relative to the light source assembly 200 and the photomultiplier assembly 300 to the light-emitting position, the collecting position and the closed position; Figure 7 As shown, in the closed position, the light emitting end 211 and the collecting end 311 are both blocked by the rotating disk 100; Figure 8 As shown, in the light-emitting position, the light-emitting end 211 is aligned with the light-emitting hole 131, and the rotating disk 100 covers the collecting end 311; Fig. 9 As shown, at the collecting position, the collecting end 311 is aligned with the collecting hole 132 , and the rotating disk 100 covers the light-emitting end 211 .

[0046] The optical system of the above-mentioned photoinduced chemiluminescence detection device of the utility model can at least achieve the following beneficial effects: Figure 8 As shown, the turntable 100 can be driven by the driving assembly 500 to rotate to the light-emitting position. At this time, the light-emitting end 211 of the light source assembly 200 is aligned with a light-emitting hole 131 and transmits the excitation light from the light-emitting hole 131 to the first end 410 of the optical fiber tube 400, and the optical fiber tube 400 then projects the excitation light from the third end 430 into the reaction chamber; Figure 7As shown, during the reaction of the reactants in the reaction chamber, the turntable 100 can be driven by the driving component 500 to rotate to the closed position to wait for the reaction to be completed. At this time, the light emitting end 211 of the light source component 200 and the collecting end 311 of the photomultiplier component 300 are both blocked by the turntable 100; Fig. 9 As shown, after the reaction is completed, the turntable 100 can be driven by the driving assembly 500 to rotate to the collection position. At this time, the collection hole 132 on the turntable 100 is aligned with the collection end 311 of the photomultiplier assembly 300, and the third end 430 of the optical fiber tube 400 can receive the signal light in the reaction chamber and transmit the signal light from the second end 420 through the collection hole 132 to the collection end 311 of the photomultiplier assembly 300. Figure 7 As shown, after the collection is completed, the turntable 100 is driven by the driving assembly 500 to rotate to the closed position again. At this time, the light-emitting end 211 of the light source assembly 200 and the collecting end 311 of the photomultiplier assembly 300 are both blocked by the turntable 100, waiting for the photomultiplier assembly 300 to cool down and prepare for the next collection work. It should be noted that the optical system of the photo-induced chemiluminescence detection device of the present application only uses a turntable 100 to realize the on-off control of multiple light paths such as the light-emitting end 211 of the light source assembly 200 and the collecting end 311 of the photomultiplier assembly 300. There is no need to set a switch structure for controlling the on-off for each light path as in the traditional solution. The structure is more streamlined, which meets the trend and demand of miniaturization of equipment, and has lower manufacturing costs.

[0047] Specifically, Figure 3 As shown, in some embodiments, the light-emitting hole 131 is embedded with an attenuation sheet 600. The attenuation sheet 600 can weaken the intensity of the excitation light emitted by the light-emitting end 211 of the light source assembly 200.

[0048] More specifically, if Figure 1 , Figure 2 and Figure 3 As shown, in some embodiments, the number of the light-emitting holes 131 is set to be multiple, and the multiple light-emitting holes 131 are arranged at intervals along the circumference of the turntable 100. Each of the light-emitting holes 131 is embedded with an attenuation sheet 600, and the attenuation coefficients of the multiple attenuation sheets 600 are different; when in the light-emitting position, the light-emitting end 211 of the light source assembly 200 is aligned with one of the light-emitting holes 131. Such a setting in this embodiment means that the attenuation sheets 600 in different light-emitting holes 131 have different weakening capabilities for the intensity of the excitation light emitted by the light-emitting end 211 of the light source assembly 200, and the light-emitting end 211 of the light source assembly 200 can be flexibly selected to be aligned with one of the light-emitting holes 131 as needed, so as to obtain excitation light of different intensities, and only one light source assembly 200 can be provided to avoid inter-unit differences.

[0049] Furthermore, if Figure 2 and Figure 4 As shown, in some embodiments, a light-shielding sleeve 700 is provided on the first surface 110 of the turntable 100, and the light-shielding sleeve 700 is arranged around the periphery of the collecting hole 132 and communicated with the collecting hole 132, and in the collecting position, the collecting end 311 is aligned with the light-shielding sleeve 700. The light-shielding sleeve 700 can prevent the influence of stray light.

[0050] Furthermore, if Figure 1 , Figure 2 , Figure 3 and Figure 4 As shown, in some embodiments, the number of the collecting holes 132 is set to be multiple, and the multiple collecting holes 132 are arranged at intervals along the circumference of the turntable 100. When in the collecting position, the collecting end 311 of the photomultiplier assembly 300 is aligned with one of the collecting holes 132.

[0051] See also Figure 3 , Figure 7 , Figure 8 and Fig. 9 In some embodiments, the light emitting hole 131 and the collecting hole 132 are spaced apart and have different rotation radii.

[0052] For example, in some embodiments, the minimum distance between the hole wall of the light-emitting hole 131 and the rotation center of the rotating disk 100 is greater than the maximum distance between the hole wall of the collecting hole 132 and the rotation center of the rotating disk 100. It is understandable that if the rotation radius of the rotation stroke of the light-emitting hole 131 is the same as the rotation radius of the rotation stroke of the collecting hole 132, the collecting hole 132 and the light-emitting end 211 of the light source assembly 200 will be temporarily docked during the rotation of the rotating disk 100, and the light-emitting hole 131 and the collecting end 311 of the photomultiplier assembly 300 will be temporarily docked, which may affect the detection. The present embodiment emphasizes that the light-emitting hole 131 and the collecting hole 132 are spaced apart and have different rotational radii, and the minimum distance between the hole wall of the light-emitting hole 131 and the rotational center of the turntable 100 is greater than the maximum distance between the hole wall of the collecting hole 132 and the rotational center of the turntable 100. Therefore, no matter how the turntable 100 rotates, the collecting hole 132 can at most align with the collecting end 311 connected to the photomultiplier assembly 300, and the light-emitting hole 131 can at most align with the light-emitting end 211 of the light source assembly 200, which will not affect the detection. In addition, another advantage of such a setting is that the number of light-emitting holes 131 that can be set on the rotational stroke where the light-emitting hole 131 is located is also greater, and will not be limited by the collecting hole 132. Similarly, the number of collecting holes 132 that can be set on the rotational stroke where the collecting hole 132 is located is also greater, and will not be limited by the light-emitting hole 131.

[0053] For another example, in some other embodiments, the minimum distance between the hole wall of the collecting hole 132 and the rotation center of the rotating disk 100 is greater than the maximum distance between the hole wall of the light emitting hole 131 and the rotation center of the rotating disk 100. It is understandable that if the rotation radius of the rotation stroke of the light emitting hole 131 is the same as the rotation radius of the rotation stroke of the collecting hole 132, the collecting hole 132 and the light emitting end 211 of the light source assembly 200 will be temporarily docked during the rotation of the rotating disk 100, and the light emitting hole 131 and the collecting end 311 of the photomultiplier assembly 300 will be temporarily docked, which may affect the detection. The present embodiment emphasizes that the light-emitting hole 131 and the collecting hole 132 are spaced apart and have different rotational radii, and the minimum distance between the hole wall of the collecting hole 132 and the rotational center of the turntable 100 is greater than the maximum distance between the hole wall of the light-emitting hole 131 and the rotational center of the turntable 100. Therefore, no matter how the turntable 100 rotates, the collecting hole 132 can at most align with the collecting end 311 connected to the photomultiplier assembly 300, and the light-emitting hole 131 can at most align with the light-emitting end 211 of the light source assembly 200, which will not affect the detection. In addition, another advantage of such a setting is that the number of light-emitting holes 131 that can be set on the rotational stroke where the light-emitting hole 131 is located is also greater, and will not be limited by the collecting hole 132. Similarly, the number of collecting holes 132 that can be set on the rotational stroke where the collecting hole 132 is located is also greater, and will not be limited by the light-emitting hole 131.

[0054] See also Figure 5 In some embodiments, the light source assembly 200 includes a laser module having the light-emitting end 211, a lens group 220 connected to the light-emitting end 211 of the laser module, and a first filter 231 connected to the lens group 220. For example, the first filter 231 only retains the 680nm wavelength excitation monochromatic light required for photoinduced chemiluminescence in the excitation light emitted by the laser module, and filters out light of other wavelengths. It should be noted that this is only for explanation and does not limit the first filter 231 to be set in this way.

[0055] See also Figure 6 In some embodiments, the photomultiplier assembly 300 includes a photomultiplier body 310 having a collecting end 311 and a second filter 322 disposed at the collecting end 311. For example, the second filter 322 only retains the monochromatic light of 620 nm wavelength required for photoinduced chemiluminescence in the signal light, and filters out the light of other wavelengths. It should be noted that this is only for explanation and does not limit the second filter 322 to be only configured in this way.

[0056] See also Figure 1 , Figure 2 and Figure 3In some embodiments, the optical system of the photo-induced chemiluminescence detection device also includes a mounting plate 800, the mounting plate 800 is located on the second surface 120 of the turntable 100, and a first through hole 810 and a second through hole 820 are opened on the mounting plate 800, the first end 410 of the optical fiber tube 400 is fixed to the mounting plate 800 and aligned with the first through hole 810, and the second end 420 of the optical fiber tube 400 is fixed to the mounting plate 800 and aligned with the second through hole 820; when in the light-emitting position, the first through hole 810 is aligned with one of the light-emitting holes 131, and the second through hole 820 is blocked by the turntable 100; when in the collecting position, the second through hole 820 is aligned with one of the collecting holes 132, and the first through hole 810 is blocked by the turntable 100; when in the closed position, both the first through hole 810 and the second through hole 820 are blocked by the turntable 100. The optical fiber tube 400 may be roughly Y-shaped, with the first end 410 and the second end 420 being bifurcated, and the third end 430 of the optical fiber tube 400 may be a converged merging end.

[0057] See also Figure 2 and Figure 3 In some embodiments, the driving assembly 500 further includes a driving motor 510 and a driving shaft 520 connected to the driving motor 510 , and one end of the driving shaft 520 is fixed to the center of the turntable 100 and is used to drive the turntable 100 to rotate.

[0058] In addition, the present application also provides a photo-induced chemiluminescence detection device, which includes a reaction chamber and an optical system as described in any of the above embodiments.

[0059] The above-mentioned photochemiluminescence detection device includes the optical system described in any of the above-mentioned embodiments, so the above-mentioned photochemiluminescence detection device also includes at least the following beneficial effects: the turntable 100 can be driven by the driving component 500 to rotate to the light-emitting position, at which time the light-emitting end 211 of the light source component 200 is aligned with a light-emitting hole 131 and transmits the excitation light from the light-emitting hole 131 to the first end 410 of the optical fiber tube 400, and the optical fiber tube 400 then projects the excitation light from the third end 430 into the reaction chamber; during the reaction process of the reactants in the reaction chamber, the turntable 100 can be driven by the driving component 500 to rotate to the light-emitting position; The turntable 100 is driven by the driving assembly 500 to rotate to the closed position to wait for the reaction to be completed. At this time, the light-emitting end 211 of the light source assembly 200 and the collecting end 311 of the photomultiplier assembly 300 are both blocked by the turntable 100. After the reaction is completed, the turntable 100 can be driven by the driving assembly 500 to rotate to the collecting position. At this time, the collecting hole 132 on the turntable 100 is aligned with the collecting end 311 of the photomultiplier assembly 300, and the third end 430 of the optical fiber tube 400 can receive the signal light in the reaction chamber, and transmit the signal light from the second end 420 to the collecting end 311 of the photomultiplier assembly 300 through the collecting hole 132. After the collection is completed, the turntable 100 is driven by the driving assembly 500 to rotate to the closed position again. At this time, the light-emitting end 211 of the light source assembly 200 and the collecting end 311 of the photomultiplier assembly 300 are both blocked by the turntable 100, waiting for the photomultiplier assembly 300 to cool down and prepare for the next collection work. It should be noted that the optical system of the photochemiluminescence detection device of the present application only uses one turntable 100 to achieve the on-off control of multiple optical paths such as the light-emitting end 211 of the light source assembly 200 and the collecting end 311 of the photomultiplier assembly 300. There is no need to set a switch structure for controlling the on-off for each optical path as in the traditional solution. The structure is more streamlined, in line with the trend and demand of miniaturization of equipment, and has lower manufacturing costs.

[0060] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0061] The above-mentioned embodiments only express several implementation methods of the utility model, and the description is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent application. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the utility model, which all belong to the protection scope of the utility model. Therefore, the protection scope of the utility model patent shall be based on the attached claims.

[0062] In the description of the present invention, it should be understood that if the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. appear, the orientation or position relationship indicated by these terms is based on the orientation or position relationship shown in the accompanying drawings, which is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0063] In addition, if the terms "first" or "second" appear, these terms are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" or "second" may explicitly or implicitly include at least one of the features. In the description of the present utility model, if the term "plurality" appears, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0064] In the present invention, unless otherwise clearly defined and limited, if the terms "installed", "connected", "connected", "fixed" and the like appear, these terms should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integrated connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to the specific circumstances.

[0065] In the present utility model, unless otherwise clearly specified and limited, if there is a description that a first feature is "above" or "below" a second feature, etc., or similar descriptions appear, it may mean that the first and second features are in direct contact, or that the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature being "above", "above" and "above" the second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. The first feature being "below", "below" and "below" the second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0066] It should be noted that if an element is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be a central element. If an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. If any, the terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used in this utility model are only for illustrative purposes and do not represent the only implementation method.

[0067] In the description of this specification, the description of reference terms such as "one embodiment", "other implementation methods" and the like means that the specific features, structures, materials or features described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic descriptions of the above terms do not necessarily refer to the same embodiment or example. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by technicians in the technical field of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention.

Claims

1. An optical system of a photoluminescence detection device, characterized in that: include: A rotating disk, wherein the rotating disk has a first surface and a second surface disposed opposite to each other along its thickness direction, and a light-emitting hole and a collecting hole are disposed through the rotating disk along the thickness direction; A light source assembly, wherein the light source assembly has a light emitting end facing the first surface; A photomultiplier assembly, wherein the photomultiplier assembly has a collecting end facing the first surface; an optical fiber tube, the optical fiber tube having a first end, a second end and a third end, the first end being used to align with the light-emitting hole from the second surface and receive the excitation light transmitted from the light-emitting hole, the third end being used to communicate with the reaction chamber and project the excitation light into the reaction chamber, and the second end being used to align with the collection hole from the second surface and transmit the signal light generated in the reaction chamber to the collection hole; and A driving assembly, the driving assembly is connected to the rotating disk and is used to drive the rotating disk to rotate relative to the light source assembly and the photomultiplier assembly to a light-emitting position, a collecting position and a closed position; At the light-emitting position, the light-emitting end is aligned with the light-emitting hole, and the rotating disk covers the collecting end; At the collecting position, the collecting end is aligned with the collecting hole, and the rotating disk covers the light-emitting end; In the closed position, both the light emitting end and the collecting end are shielded by the rotating disk.

2. The optical system of the photochemiluminescence detection device according to claim 1, characterized in that: The light-emitting hole is embedded with an attenuation sheet.

3. The optical system of the photoluminescence detection device according to claim 2, characterized in that: The number of the light-emitting holes is set to be multiple, and the multiple light-emitting holes are arranged at intervals along the circumference of the turntable. An attenuation plate is embedded in each of the light-emitting holes, and the attenuation coefficients of the multiple attenuation plates are different; when in the light-emitting position, the light-emitting end of the light source assembly is aligned with one of the light-emitting holes.

4. The optical system of the photoinduced chemiluminescence detection device according to claim 1, characterized in that: A light-proof sleeve is disposed on the first surface of the rotating disk. The light-proof sleeve is disposed around the periphery of the collecting hole and is communicated with the collecting hole. When in the collecting position, the collecting end is aligned with the light-proof sleeve.

5. The optical system of the photoinduced chemiluminescence detection device according to claim 4, characterized in that: The number of the collecting holes is set to be multiple, and the multiple collecting holes are arranged at intervals along the circumference of the rotating disk. When in the collecting position, the collecting end of the photomultiplier assembly is aligned with one of the collecting holes.

6. The optical system of the photochemiluminescence detection device according to any one of claims 1 to 5, characterized in that: The light-emitting hole and the collecting hole are arranged at intervals and have different rotation radii.

7. The optical system of the photoinduced chemiluminescence detection device according to claim 6, characterized in that: The minimum distance between the hole wall of the light-emitting hole and the rotation center of the rotating disk is greater than the maximum distance between the hole wall of the collecting hole and the rotation center of the rotating disk; Alternatively, the minimum distance between the hole wall of the collecting hole and the rotation center of the rotating disk is greater than the maximum distance between the hole wall of the light-emitting hole and the rotation center of the rotating disk.

8. The optical system of the photochemiluminescence detection device according to any one of claims 1 to 5, characterized in that: The light source assembly comprises a laser module having the light-emitting end, a lens group connected to the light-emitting end of the laser module, and a first filter connected to the lens group; And / or, the photomultiplier assembly includes a photomultiplier body having the collecting end and a second filter arranged at the collecting end.

9. The optical system of the photochemiluminescence detection device according to any one of claims 1 to 5, characterized in that: The optical system of the photoinduced chemiluminescence detection device also includes a mounting plate, the mounting plate is located on the second surface of the turntable, a first through hole and a second through hole are formed on the mounting plate, the first end of the optical fiber tube is fixed to the mounting plate and aligned with the first through hole, and the second end of the optical fiber tube is fixed to the mounting plate and aligned with the second through hole; when in the light-emitting position, the first through hole is aligned with one of the light-emitting holes, and the second through hole is blocked by the turntable; when in the collecting position, the second through hole is aligned with one of the collecting holes, and the first through hole is blocked by the turntable; when in the closed position, both the first through hole and the second through hole are blocked by the turntable; And / or, the driving assembly further includes a driving motor and a driving shaft connected to the driving motor, one end of the driving shaft is fixed to the center of the turntable and is used to drive the turntable to rotate.

10. A photoinduced chemiluminescence detection device, characterized in that: The invention comprises a reaction chamber and an optical system as claimed in any one of claims 1 to 9.

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