Self-cleaning tangential flow filtering device capable of preventing filter membrane from being blocked

Abstract

The utility model relates to a self-cleaning tangential flow filtering device capable of preventing a filter membrane from being blocked. The device comprises a filter membrane assembly, a liquid flow power assembly, a pressure detection assembly, a flow detection assembly, a flow direction switching assembly, a reagent assembly, a raw material sample loading assembly, a waste liquid collecting assembly and a liquid pipeline assembly, the liquid pipeline assembly connects the filter membrane assembly, the raw material sample loading assembly, the reagent assembly, the waste liquid collection assembly, the pressure detection assembly and the flow direction switching assembly into a pipeline system; according to the flow direction switching assembly, the reagent assembly, the waste liquid collecting assembly and the raw material sample loading assembly are connected into the pipeline system, pairwise conduction of three ports of the flow direction switching assembly is achieved through changes of the working state of the flow direction switching assembly, and different flow directions of liquid in the filtering device are switched. The self-cleaning of the filter membrane, the rinsing of the filter membrane by the buffer solution and the loading and separation of the feed liquid are respectively carried out. Compared with the prior art, the optimal separation performance of the filter membrane in the whole filtering treatment process is always kept.

Description

Technical Field

[0001] The utility model belongs to the technical field of biological molecule separation and purification, and relates to a self-cleaning tangential flow filtering device for preventing filter membrane from being blocked. Background Art

[0002] Filtration is an important method for separation and purification of biomolecules. The target product is retained by using a filter membrane with a suitable pore size to drive the liquid through the filter membrane by pressure. Among them, tangential flow filtration is a form of filtration in which the flow direction of the liquid is perpendicular to the filtration direction. During the filtration process, the liquid flow tangent to the membrane surface can flush the membrane surface to prevent the accumulation of the membrane surface to form a filter cake layer, keep the membrane surface relatively clean and work continuously for a period of time, avoid the rapid increase of filtration resistance, and achieve a higher liquid processing capacity. However, due to the porous properties of the filter membrane itself, a large number of biomolecules will still be adsorbed on the surface of the filter membrane during the filtration process, gradually blocking the filtration channel on the filter membrane and reducing the actual pore size of the filter membrane. This blockage of the filter membrane filtration channel will not only reduce the separation effect of subsequent filtration, but also reduce the actual liquid processing capacity of the filter membrane per unit area, forcing users to choose a larger area of ​​filter membrane, resulting in an increase in the cost of the separation process.

[0003] Patent CN115845612A discloses a tangential flow filtration annular system and control method, the tangential flow filtration annular system includes a tangential flow filtration assembly, a first tank body, a second tank body and a control device; the first tank body is used to store the sample liquid to be processed, the first tank body is connected to the inlet end of the tangential flow filtration assembly through a first pipeline, and the outlet end of the tangential flow filtration assembly is connected to the first tank body through a second pipeline; the second tank body is connected to the outlet end of the tangential flow filtration assembly through a third pipeline; wherein the tangential flow filtration assembly includes a tangential flow filtration membrane package, and the control device can open or close the tangential flow filtration membrane package. This patent mainly automatically selects the pore size of the filter membrane according to the molecular weight of the target protein, and the protein concentration detection device detects the protein concentration in the feed liquid in real time, and automatically determines whether the purification has reached the end point. The main problem it targets is the automatic control during the tangential flow filtration process. It fails to propose a solution for the detection of the filtration performance of the filter membrane itself and the maintenance of the optimal performance, and cannot solve the problem of performance degradation of the filter membrane due to clogging of the membrane pores during the separation of the feed liquid. Utility Model Content

[0004] The purpose of the present utility model is to overcome at least one defect of the prior art in the tangential flow filtration process, such as the blockage of the filter membrane filtration channel due to the adsorption of biological molecules and the reduction of the filtration efficiency, and to provide a self-cleaning tangential flow filtration device that prevents the filter membrane from being blocked. The utility model realizes that the optimal separation performance of the filter membrane is always maintained during the entire filtration process.

[0005] The purpose of the utility model can be achieved through the following technical solutions:

[0006] One of the technical solutions of the utility model is to provide a self-cleaning tangential flow filtration device for preventing filter membrane clogging, the device comprising a filter membrane assembly, a fluid power assembly, a pressure detection assembly, a flow detection assembly, a flow direction switching assembly, a reagent assembly, a raw material loading assembly, a waste liquid collection assembly and a liquid pipeline assembly;

[0007] The liquid pipeline component connects the filter membrane component, the raw material loading component, the reagent component, the waste liquid collection component, the pressure detection component and the flow direction switching component into a pipeline system;

[0008] The flow direction switching component connects the reagent component, the waste liquid collection component and the raw material loading component to the pipeline system respectively, realizes the two-to-two conduction of the three ports of the flow direction switching component through the change of the working state of the flow direction switching component, switches the different flow directions of the liquid in the filter device, and realizes the self-cleaning of the filter membrane, the washing of the filter membrane with the buffer solution, and the separation of the feed liquid loading respectively;

[0009] The fluid power component drives the flow of liquid in the filtration device and provides transmembrane pressure during filtration;

[0010] The filter membrane assembly is a module that directly separates and purifies the feed liquid;

[0011] The pressure detection component detects the real-time liquid flow pressure of each port of the filter membrane component respectively;

[0012] The flow detection component is used to detect the real-time liquid flow at the permeation end of the filter membrane component;

[0013] The flow direction switching component is used to select the type of liquid flowing into the pipeline system;

[0014] The liquid pipeline assembly is used to connect the liquid flow parts involved.

[0015] As a preferred technical solution, the filter membrane assembly includes a hollow fiber filter or a flat membrane package filter with four ports.

[0016] As a preferred technical solution, the pore size of the filter membrane is selected from any one of 1 kDa, 3 kDa, 5 kDa, 10 kDa, 30 kDa, 50 kDa, 70 kDa, 100 kDa, 300 kDa, 500 kDa, 750 kDa or 0.05 μm, 0.1 μm, 0.2 μm.

[0017] As a preferred technical solution, the fluid power assembly includes a three-rotor or six-rotor peristaltic pump.

[0018] As a preferred technical solution, the pressure detection component adopts a pressure sensor.

[0019] As a preferred technical solution, the flow detection component adopts a liquid flow meter.

[0020] As a preferred technical solution, the flow direction switching component adopts a three-way valve.

[0021] As a preferred technical solution, the raw material loading assembly is connected to a container containing the feed liquid.

[0022] As a preferred technical solution, the container for holding the feed liquid is a raw material bag.

[0023] As a preferred technical solution, the waste liquid collection component adopts a waste liquid collector.

[0024] As a preferred technical solution, the liquid pipeline assembly adopts a latex catheter.

[0025] Furthermore, the liquid pipeline assembly includes a first liquid pipeline assembly, a second liquid pipeline assembly, a third liquid pipeline assembly and a fourth liquid pipeline assembly, the first liquid pipeline assembly connects the injection end of the filter membrane assembly and the raw material loading assembly, the second liquid pipeline assembly connects the reflux end of the filter membrane assembly and the raw material loading assembly, the third liquid pipeline assembly connects the reagent assembly and the first liquid pipeline assembly, and the fourth liquid pipeline assembly connects the permeation end of the filter membrane assembly, the waste liquid collection assembly and the second liquid pipeline assembly.

[0026] Further, the flow direction switching component includes a first flow direction switching component, a second flow direction switching component and a third flow direction switching component;

[0027] The first liquid pipeline assembly is provided with a first flow direction switching assembly, which is respectively connected to the sampling end of the filter membrane assembly, the raw material loading assembly and the second flow direction switching assembly;

[0028] The third liquid pipeline assembly is provided with a second flow direction switching assembly, and the second flow direction switching assembly is connected to the first flow direction switching assembly and the reagent assembly respectively;

[0029] The second liquid pipeline assembly is provided with a third flow direction switching assembly, which is respectively connected to the reflux end of the filter membrane assembly, the raw material loading assembly and the waste liquid collecting assembly.

[0030] Furthermore, the reagent assembly includes a first reagent assembly and a second reagent assembly, the first reagent assembly is filled with a cleaning solution, the second reagent assembly is filled with a buffer solution, and the second flow direction switching assembly is connected to the first flow direction switching assembly, the first reagent assembly and the second reagent assembly respectively.

[0031] Furthermore, when the first flow direction switching component is in state I, the raw material loading component (raw material liquid end) and the injection end of the filter membrane component (filter membrane component injection end) are connected, and the reagent component (reagent component end) is closed;

[0032] When the first flow direction switching component is in state II, the reagent component (reagent component end) and the injection end of the filter membrane component (filter membrane component injection end) are connected, and the raw material loading component (raw material liquid end) is closed.

[0033] Furthermore, when the second flow direction switching component is in state I, the first reagent component (cleaning liquid end) and the injection end of the filter membrane component (filter membrane component injection end) are connected, and the second reagent component (buffer end) is closed;

[0034] When the second flow direction switching component is in state II, the second reagent component (buffer end) and the injection end of the filter membrane component (filter membrane component injection end) are connected, and the first reagent component (cleaning liquid end) is closed.

[0035] Furthermore, when the third flow direction switching component is in state I, the reflux end of the membrane component (the reflux end of the membrane component) and the raw material loading component (the raw material liquid end) are connected, and the waste liquid collection component (the waste liquid collection end) is closed;

[0036] When the third flow direction switching component is in state II, the reflux end of the membrane component (membrane component reflux end) and the waste liquid collection component (waste liquid collection end) are connected, and the raw material loading component (raw material liquid end) is closed.

[0037] Furthermore, the pressure detection component includes a first pressure detection component, a second pressure detection component and a third pressure detection component. The filter membrane component is provided with a first pressure detection component at the inlet end, a second pressure detection component at the reflux end, and a third pressure detection component and a flow detection component at the permeate end.

[0038] Furthermore, the device also includes a central control component and a communication connection component;

[0039] The communication connection component electrically connects the fluid power component, the pressure detection component, the flow detection component and the flow direction switching component to the central control component;

[0040] The central controller component remotely controls the flow direction switching component and the fluid power component, and the pressure detection component and the flow detection component input signals to the central control component. After analyzing the input signals, the central control component issues instructions to the flow direction switching component and the fluid power component to achieve self-cleaning and continuous filtering and processing of the feed liquid;

[0041] The central controller component records the real-time liquid flow rate at the permeation end of the filter membrane component during the entire treatment process, forms a file, and displays it to the operator through a display screen for analyzing the properties of the feed liquid, judging the separation performance and self-cleaning effect of the filter membrane, and controlling the quality of subsequent products;

[0042] The communication connection component is used to connect the circuit control part.

[0043] As a preferred technical solution, the central controller component adopts a computer.

[0044] As a preferred technical solution, the communication connection component uses a signal transmission cable and a connector.

[0045] Furthermore, the communication connection component includes a first communication connection component, a second communication connection component, a third communication connection component and a fourth communication connection component, the first communication connection component electrically connects the first pressure detection component, the second pressure detection component, the third pressure detection component and the central control component, the second communication connection component electrically connects the flow detection component and the central control component, the third communication connection component electrically connects the fluid power component and the central control component, and the fourth communication connection component electrically connects the first flow direction switching component, the second flow direction switching component, the third flow direction switching component and the central control component.

[0046] One of the technical solutions of the utility model is to provide a self-cleaning tangential flow filtration method for preventing filter membrane clogging, which is implemented by the device and comprises the following steps:

[0047] After the liquid sample separation program is executed for a period of time, the program is interrupted, and the filter membrane self-cleaning program and the buffer solution filter membrane washing program are executed. The blocked filter membrane is cleaned by the cleaning solution to restore the filter membrane separation performance, and then the liquid sample separation program is continued;

[0048] The flow detection component detects the real-time flow at the permeation end of the filter component and transmits a signal to the central control component to record the permeation end flow at the initial stage of filtration, that is, the permeation flow of the filter membrane in the best performance state, and uses this flow as the reference value;

[0049] As the separation process of the feed liquid is carried out, the filter membrane is gradually blocked, the flow rate at the permeate end gradually decreases, and the separation effect is weakened;

[0050] When the flow detection component feedback detects that the permeate flow rate drops below 60-80% of the optimal performance state benchmark value, the central control component automatically controls the fluid power component and the flow direction switching component to switch to the filter membrane self-cleaning program, and after the execution is completed, it switches to the buffer solution washing filter membrane program;

[0051] When the filter membrane self-cleaning program is running, the device automatically stops the hydraulic power component first, and then flows to the three turns in the switching component, connects the cleaning liquid to the pipeline system, connects the reflux end of the filter component to the waste liquid collection component, and closes the connection between the raw material loading component and the pipeline system. Then the hydraulic power component starts, pumps the cleaning liquid into the pipeline and enters the filter membrane component along the pipeline to clean the filter membrane and remove the adsorbed impurities that block the pores of the filter membrane.

[0052] After the membrane self-cleaning program is finished, the device automatically runs the membrane washing program with buffer solution, first stops the hydraulic power component, then turns the flow switch component to connect the buffer solution to the pipeline system, keeps the reflux end of the filter component connected to the waste liquid collection component, and keeps the connection between the raw material loading component and the pipeline system closed, then starts the hydraulic power component, pumps the buffer solution into the pipeline and enters the membrane component along the pipeline to wash the membrane, so that the membrane state is restored to be suitable for running the liquid loading separation program;

[0053] After the membrane self-cleaning program and the buffer membrane rinsing program are completed in sequence, the device automatically resumes the feed liquid loading separation program, first stops the hydraulic power component, then flows to the three switches in the switching component, closes the connection between the cleaning liquid and buffer and the pipeline system, closes the connection between the reflux end of the membrane component and the waste liquid collection component, and restores the connection between the raw material loading component and the pipeline system, then the hydraulic power component starts, continues to pump the feed liquid into the pipeline and enters the membrane component through the pipeline, continues to filter and separate the feed liquid, and through real-time monitoring of the flow rate at the permeation end of the membrane component, automatically determines whether to interrupt the feed liquid loading separation program and self-clean the membrane component.

[0054] Compared with the prior art, the utility model has the following beneficial effects:

[0055] (1) The utility model automatically determines the blockage degree and working status of the filter membrane by real-time monitoring of the flow rate at the filter membrane permeation end. When the separation performance of the filter membrane decreases, the self-cleaning program is automatically started to quickly restore the performance of the filter membrane and continue to process the feed liquid, so that the filter membrane can always separate the target product in the feed liquid with the best performance;

[0056] (2) The utility model can realize the maintenance of the optimal separation performance of the filter membrane throughout the entire filtering process, optimize the separation effect of the target product, increase the amount of liquid treated by the filter membrane per unit area, improve the treatment efficiency, and reduce the separation cost of the target product. BRIEF DESCRIPTION OF THE DRAWINGS

[0057] Figure 1 It is a structural schematic diagram of a self-cleaning tangential flow filtration device for preventing filter membrane clogging in an embodiment of the utility model;

[0058] Figure 2This is a schematic diagram of working state I of the first flow direction switching component in an embodiment of the utility model;

[0059] Figure 3 This is a schematic diagram of the working state II of the first flow direction switching component in the embodiment of the utility model;

[0060] Figure 4 This is a schematic diagram of the working state I of the second flow direction switching component in the embodiment of the utility model;

[0061] Figure 5 This is a schematic diagram of the working state II of the second flow direction switching component in the embodiment of the utility model;

[0062] Figure 6 This is a schematic diagram of working state I of the third flow direction switching component in an embodiment of the utility model;

[0063] Figure 7 This is a schematic diagram of the working state II of the third flow direction switching component in the embodiment of the utility model;

[0064] Figure 8 This is a schematic diagram of the principle of the liquid sample separation procedure in the embodiment of the utility model;

[0065] Fig. 9 This is a schematic diagram of the principle of the filter membrane self-cleaning procedure in the embodiment of the utility model;

[0066] Fig.10 It is a schematic diagram of the principle of the procedure of washing the filter membrane with buffer solution in the embodiment of the utility model.

[0067] Description of the markings in the figure:

[0068] 1—filter membrane assembly, 2—fluid flow power assembly, 3—pressure detection assembly, 3a—first pressure detection assembly, 3b—second pressure detection assembly, 3c—third pressure detection assembly, 4—flow detection assembly, 5—flow direction switching assembly, 5a—first flow direction switching assembly, 5b—second flow direction switching assembly, 5c—third flow direction switching assembly, 6—central control assembly, 7—reagent assembly, 7a—first reagent assembly, 7b—second reagent assembly, 8—raw material loading assembly, 9—waste liquid collection assembly, 10—liquid pipeline assembly, 10a—first liquid pipeline assembly, 10b—second liquid pipeline assembly, 10c—third liquid pipeline assembly, 10d—fourth liquid pipeline assembly, 11—communication connection assembly, 11a—first communication connection assembly, 11b—second communication connection assembly, 11c—third communication connection assembly, 11d—fourth communication connection assembly. DETAILED DESCRIPTION

[0069] The present invention is described in detail below in conjunction with specific embodiments. This embodiment is implemented based on the technical solution of the present invention, and provides a detailed implementation method and a specific operation process, but the protection scope of the present invention is not limited to the following embodiments.

[0070] In the description of the present utility model, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present utility model. In addition, the terms "first", "second", "third" and the like are used to describe common objects, and only refer to different instances of the same object, and are not intended to imply that the objects described in this way must adopt a given order, whether in time, space, order or any other manner.

[0071] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0072] Example:

[0073] A self-cleaning tangential flow filtration device that prevents filter membrane clogging, such as Figure 1 As shown, it includes a filter membrane component 1, a fluid power component 2, a pressure detection component 3, a flow detection component 4, a flow direction switching component 5, a central control component 6, a reagent component 7, a raw material loading component 8, a waste liquid collection component 9, a liquid pipeline component 10 and a communication connection component 11;

[0074] The liquid pipeline component 10 connects the filter membrane component 1, the raw material loading component 8, the reagent component 7, the waste liquid collection component 9, the pressure detection component 3 and the flow direction switching component 5 into a pipeline system;

[0075] The flow direction switching component 5 connects the reagent component 7, the waste liquid collection component 9 and the raw material loading component 8 to the pipeline system respectively, and realizes the two-to-two conduction of the three ports of the flow direction switching component 5 through the change of the working state of the flow direction switching component 5, switches the different flow directions of the liquid in the filter device, and realizes the self-cleaning of the filter membrane, the washing of the filter membrane with the buffer solution, and the separation of the feed liquid loading respectively;

[0076] The communication connection component 11 electrically connects the fluid power component 2, the pressure detection component 3, the flow detection component 4 and the flow direction switching component 5 to the central control component 6;

[0077] The fluid power component 2 drives the flow of liquid in the filtering device and provides transmembrane pressure during filtration;

[0078] The central controller component 6 remotely controls the flow direction switching component 5 and the fluid power component 2. The pressure detection component 3 and the flow detection component 4 input signals to the central control component 6. After analyzing the input signals, the central control component 6 sends instructions to the flow direction switching component 5 and the fluid power component 2 to achieve self-cleaning and continuous filtering and processing of the feed liquid.

[0079] The filter membrane assembly 1 is a module for directly separating and purifying the feed liquid;

[0080] The pressure detection component 3 detects the real-time liquid flow pressure of each port of the filter membrane component respectively;

[0081] The flow detection component 4 is used to detect the real-time liquid flow at the permeation end of the filter membrane component;

[0082] The flow direction switching component 5 is used to select the type of liquid flowing into the pipeline system;

[0083] The central controller component 6 records the real-time liquid flow rate at the permeation end of the filter membrane component during the entire treatment process, forms a file, and displays it to the operator through the display screen for analyzing the properties of the feed liquid, judging the separation performance and self-cleaning effect of the filter membrane, and controlling the quality of subsequent products;

[0084] The raw material loading assembly 8 is connected to a container containing the feed liquid;

[0085] The liquid pipeline assembly 10 is used to connect the liquid flow parts involved;

[0086] The communication connection component 11 is used to connect the circuit control part;

[0087] The liquid pipeline assembly 10 includes a first liquid pipeline assembly 10a, a second liquid pipeline assembly 10b, a third liquid pipeline assembly 10c and a fourth liquid pipeline assembly 10d, wherein the first liquid pipeline assembly 10a is connected to the injection end of the filter membrane assembly 1 and the raw material loading assembly 8, the second liquid pipeline assembly 10b is connected to the reflux end of the filter membrane assembly 1 and the raw material loading assembly 8, the third liquid pipeline assembly 10c is connected to the reagent assembly 7 and the first liquid pipeline assembly 10a, and the fourth liquid pipeline assembly 10d is connected to the permeation end of the filter membrane assembly 1, the waste liquid collection assembly 9 and the second liquid pipeline assembly 10b;

[0088] The flow direction switching component 5 includes a first flow direction switching component 5a, a second flow direction switching component 5b and a third flow direction switching component 5c;

[0089] The first liquid pipeline assembly 10a is provided with a first flow direction switching assembly 5a, and the first flow direction switching assembly 5a is respectively connected with the sampling end of the filter membrane assembly 1, the raw material loading assembly 8 and the second flow direction switching assembly 5b;

[0090] The third liquid pipeline assembly 10c is provided with a second flow direction switching assembly 5b, and the second flow direction switching assembly 5b is connected to the first flow direction switching assembly 5a and the reagent assembly 7 respectively;

[0091] The second liquid pipeline assembly 10b is provided with a third flow direction switching assembly 5c, and the third flow direction switching assembly 5c is connected to the reflux end of the filter membrane assembly 1, the raw material loading assembly 8 and the waste liquid collecting assembly 9 respectively;

[0092] The reagent assembly 7 includes a first reagent assembly 7a and a second reagent assembly 7b. The first reagent assembly 7a is provided with a cleaning solution, the second reagent assembly 7b is provided with a buffer solution, and the second flow direction switching assembly 5b is connected to the first flow direction switching assembly 5a, the first reagent assembly 7a and the second reagent assembly 7b respectively.

[0093] The pressure detection component 3 includes a first pressure detection component 3a, a second pressure detection component 3b and a third pressure detection component 3c. The first pressure detection component 3a is provided at the inlet end of the filter membrane component 1, the second pressure detection component 3b is provided at the reflux end, and the third pressure detection component 3c and the flow detection component 4 are provided at the permeation end;

[0094] The communication connection component 11 includes a first communication connection component 11a, a second communication connection component 11b, a third communication connection component 11c and a fourth communication connection component 11d. The first communication connection component 11a electrically connects the first pressure detection component 3a, the second pressure detection component 3b, the third pressure detection component 3c and the central control component 6, the second communication connection component 11b electrically connects the flow detection component 4 and the central control component 6, the third communication connection component 11c electrically connects the fluid power component 2 and the central control component 6, and the fourth communication connection component 11d electrically connects the first flow direction switching component 5a, the second flow direction switching component 5b, the third flow direction switching component 5c and the central control component 6.

[0095] In this embodiment, the filter membrane component 1 selects a hollow fiber filter with four ports, the pore size of the filter membrane is 100 kDa, the fluid power component 2 selects a three-rotor peristaltic pump, the pressure detection component 3 selects a pressure sensor, the flow detection component 4 selects a liquid flow meter, the flow direction switching component 5 selects a three-way valve, the central control component 6 selects a computer, the cleaning liquid is 0.5 M sodium hydroxide solution, the buffer solution is physiological saline, the container for holding the feed liquid is a raw material bag, the feed liquid is a culture medium containing the target product, the waste liquid collection component 9 selects a waste liquid collector, the liquid pipeline component 10 selects a latex catheter, and the communication connection component 11 selects a signal transmission cable and connector.

[0096] The specific working state of the flow direction switching component 5 is as follows:

[0097] like Figure 2 As shown, when the first flow direction switching component 5a is in state I, the raw material loading component 8 (raw material liquid end) and the injection end of the filter membrane component 1 (filter membrane component injection end) are connected, and the reagent component 7 (reagent component end) is closed;

[0098] like Figure 3 As shown, when the first flow direction switching component 5a is in state II, the reagent component 7 (reagent component end) and the injection end of the filter membrane component 1 (filter membrane component injection end) are connected, and the raw material loading component 8 (raw material liquid end) is closed;

[0099] like Figure 4 As shown, when the second flow direction switching component 5b is in state I, the first reagent component 7a (cleaning liquid end) and the injection end of the filter membrane component 1 (filter membrane component injection end) are connected, and the second reagent component 7b (buffer end) is closed;

[0100] like Figure 5 As shown, when the second flow direction switching component 5b is in state II, the second reagent component 7b (buffer end) and the injection end of the filter membrane component 1 (filter membrane component injection end) are connected, and the first reagent component 7a (cleaning liquid end) is closed;

[0101] like Figure 6 As shown, when the third flow direction switching component 5c is in state I, the reflux end of the membrane component 1 (the reflux end of the membrane component) and the raw material loading component 8 (the raw material liquid end) are connected, and the waste liquid collecting component 9 (the waste liquid collecting end) is closed;

[0102] like Figure 7 As shown, when the third flow direction switching component 5c is in state II, the reflux end of the membrane component 1 (membrane component reflux end) and the waste liquid collecting component 9 (waste liquid collecting end) are connected, and the raw material loading component 8 (raw material liquid end) is closed.

[0103] A self-cleaning tangential flow filtration method for preventing filter membrane clogging is implemented by the above device, and the specific steps are as follows:

[0104] First, the raw material loading component 8 is connected to the raw material bag containing the feed liquid, and then the target product in the feed liquid is separated.

[0105] like Figure 8 As shown, the feed liquid sample separation program is run: the central control component 6 controls the flow direction switching component 5 to switch, the first flow direction switching component 5a is switched to state I, the raw material liquid end and the filter membrane component injection end are connected, and the reagent component end is closed; the third flow direction switching component 5c is switched to state I, the filter membrane component reflux end and the raw material liquid end are connected, and the waste liquid collection end is closed; the central control component 6 controls the liquid flow power component 2 to rotate, drive the raw material liquid into the filter membrane component 1, filter, separate the target product and re-enter the raw material sample loading component 8 from the filter membrane component reflux end;

[0106] The flow detection component 4 detects the real-time flow at the filter membrane component through-end and transmits a signal to the central control component 6 to record the flow at the filter membrane component through-end in the initial stage of filtration, i.e., the flow at the filter membrane in the best performance state, and takes this flow as the reference value;

[0107] As the separation process of the feed liquid is carried out, the filter membrane is gradually blocked, the flow rate at the permeate end gradually decreases, and the separation effect is weakened;

[0108] When the flow detection component 4 detects that the permeation end flow rate drops below 70% of the optimal performance state reference value, the central control component 6 automatically controls the liquid sample separation program to be interrupted, first stopping the liquid flow power component 2,

[0109] like Fig. 9 As shown, the filter is then switched to the membrane self-cleaning program: the central control component 6 controls the flow switching component 5 to switch, the first flow switching component 5a switches to state II, connects the reagent component end with the filter membrane component injection end, and closes the raw material liquid end; the second flow switching component 5b switches to state I, connects the cleaning liquid end with the filter membrane component injection end, and closes the buffer end; the third flow switching component 5c switches to state II, connects the filter membrane component reflux end with the waste liquid collection end, and closes the raw material liquid end; the central control component 6 controls the liquid flow power component 2 to rotate, drives the cleaning liquid to enter the filter membrane component 1, cleans the filter membrane, removes the adsorbed impurities that block the filter membrane pores, and restores the filter membrane to the best filtering performance;

[0110] After the membrane self-cleaning program is finished, the central control component 6 first stops the fluid power component 2.

[0111] like Fig.10As shown, the automatic control switches to the buffer solution washing filter membrane procedure: the central control component 6 controls the flow direction switching component 5 to switch, the second flow direction switching component b switches to state II, connects the buffer solution end and the filter membrane component injection end, and closes the cleaning liquid end; the first flow direction switching component 5a remains in state II, connects the reagent component end and the filter membrane component injection end, and closes the raw liquid end; the third flow direction switching component 5c remains in state II, connects the filter membrane component reflux end and the waste liquid collection end, and closes the raw liquid end; the central control component 6 controls the liquid flow power component 2 to rotate, drives the buffer solution to enter the filter membrane component 1, rinses the filter membrane, and restores the filter membrane state to be suitable for running the feed liquid loading separation procedure;

[0112] After the membrane self-cleaning program and the membrane washing program are completed, the central control component 6 first stops the fluid power component 2.

[0113] Then, the automatic control is restored to run the feed liquid sample separation program: the central control component 6 controls the flow direction switching component 5 to switch, the first flow direction switching component 5a is switched to state I, the raw material liquid end and the filter membrane component injection end are connected, and the reagent component end is closed; the third flow direction switching component 5c is switched to state I, the filter membrane component reflux end and the raw material liquid end are connected, and the waste liquid collection end is closed; the central control component 6 controls the liquid flow power component 2 to rotate, drive the raw material liquid into the filter membrane component 1, filter, separate the target product and re-enter the raw material sample loading component 8 from the filter membrane component reflux end;

[0114] The flow detection component 4 still detects the real-time flow at the permeation end of the filter membrane component, and the central control component 6 automatically determines whether to interrupt the liquid sample separation program and self-clean the filter membrane component 1;

[0115] Through the cycle of feed liquid loading separation program → filter membrane self-cleaning program → buffer solution rinsing filter membrane program → feed liquid loading separation program, when the separation performance of the filter membrane decreases, the self-cleaning program is automatically started, and the feed liquid processing is continued after the filter membrane performance is quickly restored, so that the filter membrane can always separate the target product in the feed liquid with the best performance, and finally the target product is harvested in the raw material loading component 8.

[0116] The above description of the embodiments is to facilitate the understanding and use of the utility model by those skilled in the art. It is obvious that those familiar with the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative work. Therefore, the utility model is not limited to the above embodiments, and improvements and modifications made by those skilled in the art based on the disclosure of the utility model without departing from the scope of the utility model should be within the scope of protection of the utility model.

Claims

1. A self-cleaning tangential flow filtration device for preventing filter membrane clogging, characterized in that: The device comprises a filter membrane component (1), a fluid power component (2), a pressure detection component (3), a flow detection component (4), a flow direction switching component (5), a reagent component (7), a raw material loading component (8), a waste liquid collection component (9) and a liquid pipeline component (10); The liquid pipeline component (10) connects the filter membrane component (1), the raw material loading component (8), the reagent component (7), the waste liquid collection component (9), the pressure detection component (3) and the flow direction switching component (5) into a pipeline system; The flow direction switching component (5) connects the reagent component (7), the waste liquid collection component (9) and the raw material loading component (8) to the pipeline system respectively, and realizes the connection between the three ports of the flow direction switching component (5) by changing the working state of the flow direction switching component (5), switches the different flow directions of the liquid in the filter device, and realizes the self-cleaning of the filter membrane, the washing of the filter membrane with the buffer solution, and the separation of the feed liquid loading.

2. A self-cleaning tangential flow filtration device for preventing filter membrane clogging according to claim 1, characterized in that: The liquid pipeline assembly (10) comprises a first liquid pipeline assembly (10a), a second liquid pipeline assembly (10b), a third liquid pipeline assembly (10c) and a fourth liquid pipeline assembly (10d); the first liquid pipeline assembly (10a) is connected to the injection end of the filter membrane assembly (1) and the raw material loading assembly (8); the second liquid pipeline assembly (10b) is connected to the reflux end of the filter membrane assembly (1) and the raw material loading assembly (8); the third liquid pipeline assembly (10c) is connected to the reagent assembly (7) and the first liquid pipeline assembly (10a); and the fourth liquid pipeline assembly (10d) is connected to the permeation end of the filter membrane assembly (1), the waste liquid collection assembly (9) and the second liquid pipeline assembly (10b).

3. A self-cleaning tangential flow filtration device for preventing filter membrane clogging according to claim 2, characterized in that: The flow direction switching component (5) comprises a first flow direction switching component (5a), a second flow direction switching component (5b) and a third flow direction switching component (5c); The first liquid pipeline component (10a) is provided with a first flow direction switching component (5a), and the first flow direction switching component (5a) is respectively connected to the sampling end of the filter membrane component (1), the raw material loading component (8) and the second flow direction switching component (5b); The third liquid pipeline component (10c) is provided with a second flow direction switching component (5b), and the second flow direction switching component (5b) is connected to the first flow direction switching component (5a) and the reagent component (7) respectively; The second liquid pipeline component (10b) is provided with a third flow direction switching component (5c), and the third flow direction switching component (5c) is respectively connected to the reflux end of the filter membrane component (1), the raw material loading component (8) and the waste liquid collection component (9).

4. A self-cleaning tangential flow filtration device for preventing filter membrane clogging according to claim 3, characterized in that: The reagent assembly (7) comprises a first reagent assembly (7a) and a second reagent assembly (7b); the first reagent assembly (7a) is provided with a cleaning solution, the second reagent assembly (7b) is provided with a buffer solution, and the second flow direction switching assembly (5b) is respectively connected to the first flow direction switching assembly (5a), the first reagent assembly (7a) and the second reagent assembly (7b).

5. A self-cleaning tangential flow filtration device for preventing filter membrane clogging according to claim 3, characterized in that: When the first flow direction switching component (5a) is in state I, the raw material loading component (8) and the injection end of the filter membrane component (1) are connected, and the reagent component (7) is closed; When the first flow direction switching component (5a) is in state II, the reagent component (7) and the sample injection end of the filter membrane component (1) are connected, and the raw material loading component (8) is closed.

6. A self-cleaning tangential flow filtration device for preventing filter membrane clogging according to claim 3, characterized in that: When the second flow direction switching component (5b) is in state I, the first reagent component (7a) and the sample injection end of the filter membrane component (1) are connected, and the second reagent component (7b) is closed; When the second flow direction switching component (5b) is in state II, the second reagent component (7b) and the injection end of the filter membrane component (1) are connected, and the first reagent component (7a) is closed.

7. A self-cleaning tangential flow filtration device for preventing filter membrane clogging according to claim 3, characterized in that: When the third flow direction switching component (5c) is in state I, the reflux end of the filter membrane component (1) and the raw material loading component (8) are connected, and the waste liquid collection component (9) is closed; When the third flow direction switching component (5c) is in state II, the reflux end of the filter membrane component (1) and the waste liquid collection component (9) are connected, and the raw material loading component (8) is closed.

8. A self-cleaning tangential flow filtration device for preventing filter membrane clogging according to claim 1, characterized in that: The pressure detection component (3) comprises a first pressure detection component (3a), a second pressure detection component (3b) and a third pressure detection component (3c); the filter membrane component (1) is provided with the first pressure detection component (3a) at the injection end, the second pressure detection component (3b) at the reflux end, and the third pressure detection component (3c) and the flow detection component (4) at the permeation end.

9. A self-cleaning tangential flow filtration device for preventing filter membrane clogging according to claim 1, characterized in that: The device also includes a central control component (6) and a communication connection component (11); The communication connection component (11) electrically connects the fluid power component (2), the pressure detection component (3), the flow detection component (4) and the flow direction switching component (5) to the central control component (6).

10. A self-cleaning tangential flow filtration device for preventing filter membrane clogging according to claim 9, characterized in that: The communication connection component (11) comprises a first communication connection component (11a), a second communication connection component (11b), a third communication connection component (11c) and a fourth communication connection component (11d); the first communication connection component (11a) is electrically connected to the first pressure detection component (3a), the second pressure detection component (3b), the third pressure detection component (3c) and the central control component (6); the second communication connection component (11b) is electrically connected to the flow detection component (4) and the central control component (6); the third communication connection component (11c) is electrically connected to the fluid power component (2) and the central control component (6); and the fourth communication connection component (11d) is electrically connected to the first flow direction switching component (5a), the second flow direction switching component (5b), the third flow direction switching component (5c) and the central control component (6).

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