Strain tube structure
By designing a strain tube structure including strain tubes, connecting puncture structures, culture medium tubes, inoculation rings and inoculation ring handles, the problem of cumbersome operation and contamination risks of laboratory self-preservation of strains is solved, and pollution-free and efficient strain inoculation and sampling operations are achieved.
Patent Information
- Application Number
- CN202421447549.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-24
- Publication Date
- 2025-05-02
- Estimated Expiration
- 2034-06-24
AI Technical Summary
In the prior art, the method of labs to preserve bacterial strains by themselves is complicated and there is a risk of bacterial strain contamination.
A strain tube structure is designed, including strain tube, connecting puncture structure, culture medium tube, inoculation ring and inoculation ring handle. It is connected by threaded closed connection, and built-in inoculation ring and inoculation ring handle to reduce the risk of preparation and contamination.
The pollution-free operation of the vaccination and sampling process is achieved, reducing operational complexity and pollution risks, and improving operational convenience and safety.
Smart Images
Figure CN222821561U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of microbial inspection in the food, medicine and medical device industries, in particular to the field of laboratory bacteria cultivation, and specifically refers to a bacterial strain tube structure. Background Art
[0002] In the fields of medicine, food, medical devices, etc., microbial testing is involved in the production and factory inspection of products. It is inevitable to use positive strains in microbial testing, or to conduct culture medium suitability testing, or to conduct positive controls. At present, there are two main methods for using strains. One is a one-time quantitative strain, where each strain has a certain number of bacteria, is packaged separately, and is discarded after use; the other is that the laboratory purchases strains, amplifies and preserves them by itself, and the frozen strains in the laboratory need to be revived before each use.
[0003] The former is safe and convenient but the cost is high, and laboratory storage is still the choice of most companies. However, in addition to being cumbersome to operate, this method also has the risk of bacterial contamination.
[0004] The process of bacterial strain subculture is as follows: open the primary bacterial strain package, add physiological saline to dissolve, transfer the dissolved bacterial solution to the expansion medium, culture at an appropriate temperature, streak the cultured bacterial solution on the slant medium, culture, and refrigerate the cultured slant strains in a 4°C refrigerator.
[0005] This process involves opening the culture storage environment multiple times to introduce or transfer bacterial liquid. Contamination may occur if any of the processes is not handled properly. Utility Model Content
[0006] The purpose of the utility model is to overcome the shortcomings of the prior art and provide a bacterial seed tube structure which has the advantages of simple structure, high convenience and wide application range.
[0007] In order to achieve the above purpose, the structure of the spawn tube of the utility model is as follows:
[0008] The main features of the bacterial strain tube structure are that the structure includes a bacterial strain tube, a connecting puncture structure, a culture medium tube, an inoculation loop and an inoculation loop handle; the opening of the bacterial strain tube is sealed by an aluminum film, the culture medium tube is a two-way opening, the lower end opening of the culture medium tube is sealed by an aluminum film, the connecting puncture structure is connected between the bacterial strain tube and the culture medium tube, a sharp thorn is provided in the middle of the connecting puncture structure, one end of the inoculation loop is connected to the inoculation loop handle, the inoculation loop is inserted into the culture medium tube, the inoculation loop handle is located at the upper end opening of the culture medium tube and is sealed, and serves as a tube cap for the culture medium tube.
[0009] Preferably, the opening of the bacterial tube is threaded, the openings at both ends of the culture medium tube are threaded, and the interior of the connecting puncture structure is threaded, corresponding to the threads of the bacterial tube and the threads at the lower end opening of the culture medium tube.
[0010] Preferably, the inoculation loop handle has threads corresponding to the threads at the upper opening of the culture medium tube.
[0011] Preferably, the height of the spikes of the connection puncture structure is 1 / 5 of the height of the connection puncture structure.
[0012] Preferably, the sharp thorns of the connection puncture structure can pierce the aluminum film at the opening of the culture tube and the aluminum film at the lower end opening of the culture medium tube.
[0013] Preferably, the bacterial strain tube is loaded with freeze-dried bacterial strains, and the culture medium tube is loaded with culture medium. After the sharp thorns of the connecting puncture structure pierce the aluminum film at the opening of the bacterial strain tube and the aluminum film at the lower end opening of the culture medium tube, the culture medium in the culture medium tube is sucked into the bacterial strain tube containing the freeze-dried bacterial strains.
[0014] The bacterial strain tube structure of the utility model is adopted. The bacterial strain tube is connected to the culture structure through a threaded and sealed connection, and there is no risk of contamination during the inoculation process. The built-in inoculation loop reduces the preparation work, and the bacterial strain is obtained when the inoculation loop is taken out, reducing the risk of contamination in the inoculation process. The design of the inoculation ring handle and nut can change the air intake during bacterial culture by tightening or loosening, especially after fully tightening, no air enters, which is extremely critical for the growth of anaerobic bacteria. The bacterial strain tube provides the original bacterial strain and the inoculation loop, which solves the contamination risk in the inoculation and sampling links. It has a wide range of uses and is more convenient for operators. It is operated in a closed structure, and there is no risk of bacterial strain contamination and bacterial strain infection to personnel. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] Figure 1 It is a structural schematic diagram of the bacterial seed tube structure of the utility model.
[0016] Reference numerals:
[0017] 1 Seed tube
[0018] 2 Connect the puncture structure
[0019] 3 Culture medium tubes
[0020] 4 Inoculation loop
[0021] 5 Inoculation loop handle DETAILED DESCRIPTION
[0022] In order to more clearly describe the technical content of the present invention, further description is given below in conjunction with specific embodiments.
[0023] The bacterial strain tube structure of the utility model comprises a bacterial strain tube, a connecting puncture structure, a culture medium tube, an inoculation loop and an inoculation loop handle. The opening of the bacterial strain tube is sealed by an aluminum film, the culture medium tube is a two-way opening, the lower end opening of the culture medium tube is sealed by an aluminum film, the connecting puncture structure is connected between the bacterial strain tube and the culture medium tube, a sharp thorn is arranged in the middle position of the connecting puncture structure, one end of the inoculation loop is connected to the inoculation loop handle, the inoculation loop is inserted into the culture medium tube, the inoculation loop handle is located at the upper end opening of the culture medium tube and is sealed, and serves as a tube cap of the culture medium tube.
[0024] As a preferred embodiment of the utility model, the opening of the bacterial culture tube is threaded, the openings at both ends of the culture medium tube are threaded, and the interior of the connecting puncture structure is threaded, and corresponds to the thread of the bacterial culture tube and the thread at the lower end opening of the culture medium tube.
[0025] As a preferred embodiment of the utility model, the inoculation loop handle has threads corresponding to the threads at the upper opening of the culture medium tube.
[0026] As a preferred embodiment of the present invention, the height of the spikes of the connecting puncture structure is 1 / 5 of the height of the connecting puncture structure.
[0027] As a preferred embodiment of the utility model, the sharp thorns of the connecting puncture structure can pierce the aluminum film at the opening of the culture tube and the aluminum film at the lower end opening of the culture medium tube.
[0028] As a preferred embodiment of the utility model, the culture tube is loaded with freeze-dried culture, and the culture medium tube is loaded with culture medium. After the sharp thorns of the connecting puncture structure pierce the aluminum film at the opening of the culture tube and the aluminum film at the lower end opening of the culture medium tube, the culture medium of the culture medium tube is sucked into the culture tube containing the freeze-dried culture.
[0029] In a specific implementation manner of the utility model, freeze-dried bacteria are loaded into a bacteria tube, the bacteria tube is a one-way opening, the opening is provided with threads, and is sealed with an aluminum film. Figure 1 The medium culture tube is purple.
[0030] The culture medium is placed in a transparent culture medium tube, which has a two-way opening, threads on both sides of the opening, and is sealed with an aluminum film on the bottom.
[0031] The inoculation loop handle has threads corresponding to the threads of the culture medium tube. After the inoculation loop is inserted into the culture medium tube, the threads are tightened. At this time, the inoculation loop handle acts as a cap for the culture medium tube, and the culture medium tube is sealed.
[0032] Figure 1The medium blue part is a connecting puncture structure with internal threads, which correspond to the threads on the upper side of the culture tube and the threads on the lower side of the culture medium tube. The culture medium tube and the strain tube are connected by threads. There is a thorn in the middle position, which is continuously screwed into the culture tube and the culture medium tube. The thorn can pierce the aluminum film that seals the two.
[0033] The scratching device of the culture tube is a plastic spike, which is located in the middle of the connecting device and has a height of only 1 / 5 of the connecting device, and will never hurt the operator.
[0034] When using, first rotate the purple bacterial strain hose upwards, and the aluminum film of the hose will be pierced by the preset puncture needle in the blue part, then rotate the transparent culture medium tube downwards, and the aluminum film of the culture medium tube will also be pierced by the preset puncture needle. At this time, the pipeline is connected, and the bacterial strain hose is squeezed repeatedly, and the liquid above will be sucked into the hose containing the bacterial strain below and mixed evenly. At this time, the operation is completed and the culture can be carried out. After culture, the product comes with an inoculation loop, which can be used for bevel marking.
[0035] By using this strain tube, most operations in the strain preservation process are completed in a closed environment, which can greatly avoid contamination, and the user does not need to prepare consumables such as physiological saline, culture medium, inoculation loop, etc., thus reducing the workload.
[0036] The specific implementation scheme of this embodiment can refer to the relevant description in the above embodiment, which will not be repeated here.
[0037] It can be understood that the same or similar parts of the above embodiments can be referenced to each other, and the contents not described in detail in some embodiments can refer to the same or similar contents in other embodiments.
[0038] It should be noted that, in the description of the present invention, the terms "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying relative importance. In addition, in the description of the present invention, unless otherwise specified, the meaning of "plurality" refers to at least two.
[0039] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the utility model. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.
[0040] The bacterial strain tube structure of the utility model is adopted. The bacterial strain tube is connected to the culture structure through a threaded and sealed connection, and there is no risk of contamination during the inoculation process. The built-in inoculation loop reduces the preparation work, and the bacterial strain is obtained when the inoculation loop is taken out, reducing the risk of contamination in the inoculation process. The design of the inoculation ring handle and nut can change the air intake during bacterial culture by tightening or loosening, especially after fully tightening, no air enters, which is extremely critical for the growth of anaerobic bacteria. The bacterial strain tube provides the original bacterial strain and the inoculation loop, which solves the contamination risk in the inoculation and sampling links. It has a wide range of uses and is more convenient for operators. It is operated in a closed structure, and there is no risk of bacterial strain contamination and bacterial strain infection to personnel.
[0041] In this specification, the utility model has been described with reference to its specific embodiments. However, it is obvious that various modifications and changes can be made without departing from the spirit and scope of the utility model. Therefore, the description and drawings should be regarded as illustrative rather than restrictive.
Claims
1. A bacterial seed tube structure, characterized in that: The structure includes a strain tube, a connecting puncture structure, a culture medium tube, an inoculation loop and an inoculation loop handle. The opening of the strain tube is sealed by an aluminum film, the culture medium tube is a two-way opening, the lower end opening of the culture medium tube is sealed by an aluminum film, the connecting puncture structure is connected between the strain tube and the culture medium tube, and a sharp thorn is provided in the middle of the connecting puncture structure. One end of the inoculation loop is connected to the inoculation loop handle, the inoculation loop is inserted into the culture medium tube, and the inoculation loop handle is located at the upper end opening of the culture medium tube and is sealed to serve as a tube cap for the culture medium tube.
2. The bacterial seed tube structure according to claim 1, characterized in that: The opening of the bacterial tube is threaded, the openings at both ends of the culture medium tube are threaded, and the interior of the connecting puncture structure is threaded, corresponding to the thread of the bacterial tube and the thread at the lower end opening of the culture medium tube.
3. The bacterial seed tube structure according to claim 2, characterized in that: The handle of the inoculation loop is provided with threads, which correspond to the threads at the opening of the upper end of the culture medium tube.
4. The bacterial seed tube structure according to claim 1, characterized in that: The height of the spikes of the connection puncture structure is 1 / 5 of the height of the connection puncture structure.
5. The bacterial seed tube structure according to claim 1, characterized in that: The sharp thorns of the connection puncture structure can pierce the aluminum film at the opening of the bacterial seed tube and the aluminum film at the lower end opening of the culture medium tube.
6. The bacterial seed tube structure according to claim 1, characterized in that: The strain tube is filled with freeze-dried strains, and the culture medium tube is filled with culture medium. After the sharp thorns of the connecting puncture structure pierce the aluminum film at the opening of the strain tube and the aluminum film at the lower opening of the culture medium tube, the culture medium in the culture medium tube is sucked into the strain tube containing the freeze-dried strains.