Detection assembly for nucleic acid detection and nucleic acid analysis device

What is AI technical title?
AI technical title is built by Patsnap AI team. It summarizes the technical point description of the patent document.
By designing detection components for nucleic acid detection, including a rotary heating mechanism and a compact detection box, the problems of poor heating effect and large land use of existing nucleic acid detectors are solved, and more efficient and accurate nucleic acid detection is achieved.

CN222821567UActive Publication Date: 2025-05-02QINGDAO AGRI UNIV +1

View PDF 0 Cites 0 Cited by

Patent Information

Application Number: CN202421632704.6
Authority / Receiving Office: CN · China
Patent Type: Utility models(China)
Current Assignee / Owner
Filing Date: 2024-07-11
Publication Date: 2025-05-02
Estimated Expiration: 2034-07-11

AI Technical Summary

Technical Problem

The existing nucleic acid detectors have poor and uneven heating effects on test tubes, which affects the detection results, is not very integrated and covers a large area.

Method used

A detection assembly for nucleic acid detection is designed, including a detection chamber and a heating assembly. The heating assembly is rotated by driving the placement rack through the rotary table to ensure that the sample tube is heated evenly in the heating cover. At the same time, the compact structure of the inspection box and the design of the handling tank improve the convenience of use.

Benefits of technology

The uniformity and efficiency of test tube heating are achieved, the accuracy and efficiency of nucleic acid detection are improved, and the floor area of the equipment is reduced and the convenience is improved.

✦ Generated by Eureka AI based on patent content.

Smart Images

Figure CN222821567U_ABST

Patent Text Reader

Abstract

The utility model relates to the technical field of nucleic acid detection, in particular to a detection assembly for nucleic acid detection and a nucleic acid analysis device.The detection assembly comprises a detection box body, a containing cavity is formed in the right end of the detection box body, and a heating assembly is fixedly installed in the containing cavity; the heating assembly comprises a base fixedly installed in the containing cavity, the top of the base is rotationally connected with a rotating table, a placing frame is fixedly installed at the top of the rotating table, multiple sets of sample test tubes are placed on the inner side of the placing frame, and a heating cover is movably installed at the position, located on the outer side of the placing frame, of the top of the base; compared with the existing nucleic acid detector, the nucleic acid detector disclosed by the utility model has the advantages that the structure is compact, the whole occupied area is small, the use convenience is greatly improved, and meanwhile, the accuracy of a detection result and the detection efficiency are guaranteed.

Need to check novelty before this filing date? Find Prior Art

Description

Technical Field

[0001] The utility model relates to the technical field of nucleic acid detection, and in particular to a detection component and a nucleic acid analysis device for nucleic acid detection. Background Art

[0002] Nucleic acid is a biological macromolecule composed of many nucleotides. It is one of the most basic substances of life. Nucleic acid is widely present in all animal and plant cells and microorganisms. Nucleic acid in organisms often combines with proteins to form nucleoproteins. Different nucleic acids have different chemical compositions and nucleotide arrangement sequences. When extracting nucleic acids, proteases need to be added to the sample solution to remove intracellular proteins.

[0003] Enzymes need a suitable temperature to speed up the reaction. Existing nucleic acid detectors usually only heat the bottom of the test tube, and the heating effect is poor and uneven, which can easily cause the protein and RNA inside the test tube to not be completely decomposed, affecting the test results. In addition, the existing nucleic acid detectors are not highly integrated and occupy a large area.

[0004] Therefore, it is particularly important to design a detection component for nucleic acid detection and a nucleic acid analysis device to solve the above-mentioned defects. Utility Model Content

[0005] In view of the shortcomings of the prior art, the utility model designs a detection component and a nucleic acid analysis device for nucleic acid detection, which aim to solve the technical problems that the nucleic acid detector in the prior art has poor and uneven heating effect on the test tube, affecting the detection results, and the existing nucleic acid detector has low integration and occupies a large area.

[0006] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0007] A detection component for nucleic acid detection, comprising a detection box, a receiving cavity is provided at the right end of the detection box, and a heating component is fixedly installed inside the receiving cavity;

[0008] The heating assembly includes a base fixedly installed inside the accommodating cavity, the top of the base is rotatably connected to a rotating table, the top of the rotating table is fixedly installed with a placement rack, a plurality of groups of sample test tubes are placed inside the placement rack, a heating cover is movably installed on the top of the base and outside the placement rack, and two groups of heating tubes are fixedly installed on the inner wall of the heating cover.

[0009] As a preferred solution of the utility model, the left and right sides of the detection box are provided with conveying grooves, the top of the detection box and the outside of the accommodating cavity are rotatably connected with a sealing cover, and the inner side of the sealing cover is fixedly connected with a sealing gasket.

[0010] As a preferred solution of the utility model, a first motor is fixedly installed on the right end of the bottom of the base, a driving gear is fixedly installed on the driving end of the first motor, a driven gear is rotatably connected to the top of the base, and the driven gear is meshed with the driving gear, a turntable is fixedly connected to the top of the driven gear, a toggle block is fixedly connected to the top of the turntable, and a plurality of toggle grooves adapted for use with the toggle blocks are provided on the outer side of the rotating table.

[0011] As a preferred solution of the utility model, a buckle groove is opened at the center of the top of the heating hood, the top of the heating hood is rotatably connected to a movable cover, the top of the heating hood and in front of the movable cover is rotatably connected to a first electric telescopic rod, and the driving end of the first electric telescopic rod is rotatably connected to the movable cover.

[0012] The utility model also provides a nucleic acid analysis device, comprising any one of the above-mentioned detection components for nucleic acid detection, and further comprising:

[0013] The nucleic acid detector body is fixedly installed inside the left end of the detection box, and a pipetting component is fixedly installed on the right end of the top of the nucleic acid detector body.

[0014] As a preferred solution of the present utility model, an operation display area is provided at the front end of the nucleic acid detection instrument body, and a detection port is opened at the left end of the top of the nucleic acid detection instrument body.

[0015] As a preferred solution of the utility model, the pipetting assembly includes a fixing seat fixedly mounted on the top of the nucleic acid detection instrument body, a second motor is fixedly mounted on the top of the fixing seat, a cantilever is fixedly mounted on the driving end of the second motor, a second electric telescopic rod is fixedly mounted on the right end of the cantilever, and a pipette gun is fixedly mounted on the driving end of the second electric telescopic rod.

[0016] Compared with the prior art, the beneficial effects of the utility model are:

[0017] In the utility model, the detection box, the heating component, the nucleic acid detector body and the pipetting component are designed in coordination, and then the sample test tube is placed on the inner side of the placement rack and the heating cover is covered, the heating tube in the heating cover is started, and the placement rack is driven by the rotating table to rotate and heat, which can not only heat evenly to ensure the detection results, but also improve the reaction rate, thereby improving the efficiency of nucleic acid detection;

[0018] The internal structure of the detection box is compact and the overall footprint is small. At the same time, it can be transported through the transport slot, which greatly improves the convenience of use;

[0019] When the sample solution is subsequently tested and analyzed, in order to avoid contamination of the sample tube caused by opening the entire heating cover, when taking out the sample solution in different sample tubes, the corresponding sample tube is first moved to the position of the movable cover by rotating the turntable, and then the first electric telescopic rod is started to open the movable cover, and the sample solution is transferred to the interior of the nucleic acid detector body for testing and analysis using the pipetting assembly, thereby further ensuring the accuracy of the test results. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0021] Figure 2 This is a schematic diagram of the structure of the heating component of the utility model;

[0022] Figure 3 This is a partial structural diagram of the heating component of the utility model;

[0023] Figure 4 This is a schematic diagram of the structure of the heating cover of the utility model.

[0024] In the figure: 1. detection box; 101. conveying slot; 102. sealing cover; 103. sealing gasket; 2. accommodating chamber; 3. heating component; 301. base; 302. rotating table; 303. placement rack; 304. sample test tube; 305. heating cover; 306. heating tube; 307. first motor; 308. driving gear; 309. driven gear; 310. turntable; 311. toggle block; 312. toggle slot; 313. buckle slot; 314. movable cover; 315. first electric telescopic rod; 4. nucleic acid detection instrument body; 401. operation display area; 402. detection port; 5. pipetting component; 501. fixing seat; 502. second motor; 503. cantilever; 504. second electric telescopic rod; 505. pipetting gun. DETAILED DESCRIPTION

[0025] The technical solutions in the embodiments of the utility model will be clearly and completely described below in conjunction with the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the utility model.

[0026] Example:

[0027] See also Figure 1-Figure 4 , the utility model provides a technical solution:

[0028] A detection component for nucleic acid detection, comprising a detection box 1, a receiving cavity 2 is provided at the right end of the detection box 1, and a heating component 3 is fixedly installed inside the receiving cavity 2;

[0029] First, in this embodiment, the specific structure of the heating component 3 is as follows:

[0030] The heating assembly 3 includes a base 301 fixedly installed inside the accommodating chamber 2, the top of the base 301 is rotatably connected to a rotating table 302, the top of the rotating table 302 is fixedly installed with a placement rack 303, a plurality of groups of sample test tubes 304 are placed on the inner side of the placement rack 303, a heating cover 305 is movably installed on the top of the base 301 and located on the outer side of the placement rack 303, two groups of heating tubes 306 are fixedly installed on the inner side wall of the heating cover 305, the heating cover 305 is first opened, then protease is added to the sample test tube 304 containing the sample solution, and then the sample test tube 304 is placed on the inner side of the placement rack 303 and the heating cover 305 is covered, the heating tube 306 in the heating cover 305 is started, and the placement rack 303 is driven to rotate by the rotating table 302, so that the plurality of groups of sample test tubes 304 can not only be evenly heated on the inner side of the heating cover 305 to ensure the detection results, but also improve the reaction rate.

[0031] Furthermore, a conveying groove 101 is provided on both the left and right sides of the detection box 1, a sealing cover 102 is rotatably connected to the top of the detection box 1 and located on the outside of the accommodating chamber 2, and a sealing gasket 103 is fixedly connected to the inner side of the sealing cover 102. The internal structure of the detection box 1 is compact and the overall footprint is small. At the same time, it can be transported and used through the conveying groove 101, which greatly improves the convenience of use. At the same time, during the heating of the sample test tube 304, the sealing cover 102 is covered and its sealing is ensured by the sealing gasket 103, so that the heat of the heating cover 305 is not easily dissipated, thereby ensuring the heating effect.

[0032] Then, a first motor 307 is fixedly installed at the right end of the bottom of the base 301, and a driving gear 308 is fixedly installed on the driving end of the first motor 307. A driven gear 309 is rotatably connected to the top of the base 301, and the driven gear 309 is meshed with the driving gear 308. A turntable 310 is fixedly connected to the top of the driven gear 309, and a toggle block 311 is fixedly connected to the top of the turntable 310. A plurality of toggle grooves 312 adapted for use with the toggle block 311 are provided on the outer side of the rotating table 302. In the process of heating the sample test tube 304, the first motor 307 at the bottom of the base 301 is started to drive the driving gear 308 to rotate, and the toggle block 311 on the top of the turntable 310 enters the toggle groove 312 under the transmission of the driven gear 309, thereby driving the rotating table 302 to rotate, so that the plurality of sample test tubes 304 can be evenly heated and the reaction rate is improved.

[0033] Furthermore, a buckle groove 313 is provided at the center of the top of the heating hood 305, and the top of the heating hood 305 is rotatably connected to a movable cover 314, and the top of the heating hood 305 and the front of the movable cover 314 are rotatably connected to a first electric telescopic rod 315, and the driving end of the first electric telescopic rod 315 is rotatably connected to the movable cover 314 through the buckle groove 313, so that the heating hood 305 can be easily taken in and out. When the sample solution is subsequently tested and analyzed, in order to avoid contamination of the sample tube 304 by opening the entire heating hood 305, when taking out the sample solution in different sample tubes 304, the corresponding sample tube 304 is first moved to the position of the movable cover 314 by rotating the rotating table 302, and then the first electric telescopic rod 315 is started to open the movable cover 314, so as to take out the required sample solution to avoid contamination of other sample solutions, thereby further ensuring the accuracy of the test results.

[0034] The utility model also provides a nucleic acid analysis device, which also includes:

[0035] The nucleic acid detector body 4 is fixedly installed inside the left end of the detection box 1, and a pipetting component 5 is fixedly installed on the right end of the top of the nucleic acid detector body 4. The sample solution after the reaction is transferred to the interior of the nucleic acid detector body 4 through the pipetting component 5, and then the nucleic acid detector body 4 is used to detect and obtain the result.

[0036] Among them, an operation display area 401 is provided at the front end of the nucleic acid detection instrument body 4, and a detection port 402 is opened at the left end of the top of the nucleic acid detection instrument body 4. The nucleic acid detection instrument body 4 is operated through the operation display area 401 and the detection results are observed and recorded. The sample solution is transferred to the detection port 402 using the pipetting component 5, so that the results are obtained through the detection and analysis of the nucleic acid detection instrument body 4.

[0037] Furthermore, the pipetting assembly 5 includes a fixing base 501 fixedly mounted on the top of the nucleic acid detection instrument body 4, a second motor 502 is fixedly mounted on the top of the fixing base 501, a cantilever 503 is fixedly mounted on the driving end of the second motor 502, a second electric telescopic rod 504 is fixedly mounted on the right end of the cantilever 503, and a pipette gun 505 is fixedly mounted on the driving end of the second electric telescopic rod 504. After the movable cover 314 is opened, the second motor 502 is started to swing the cantilever 503 to above the movable cover 314, and then the second electric telescopic rod 504 is started to lower the pipette gun 505, and the sample solution is transferred to the interior of the nucleic acid detection instrument body 4 through the pipette gun 505 for detection and analysis.

[0038] In this embodiment, the implementation scenario is specifically as follows: first, the heating cover 305 is opened, and then protease is added to the sample test tube 304 containing the sample solution, and then the sample test tube 304 is placed on the inner side of the placement rack 303 and then the heating cover 305 is covered, and the heating tube 306 in the heating cover 305 is started, and the placement rack 303 is driven to rotate by the rotating table 302, so that multiple groups of sample test tubes 304 can not only be evenly heated on the inner side of the heating cover 305 to ensure the detection results, but also can improve the reaction rate. When the sample solution is subsequently detected and analyzed, in order to avoid contamination of the sample test tube 304 caused by opening the entire heating cover 305, when taking out different sample test tubes 304 When the sample solution is collected, the corresponding sample test tube 304 is first moved to the position of the movable cover 314 by rotating the rotating table 302, and then the first electric telescopic rod 315 is started to open the movable cover 314, and the second motor 502 is started to swing the cantilever 503 to above the movable cover 314, and then the second electric telescopic rod 504 is started to lower the pipette 505, and the sample solution is transferred to the interior of the nucleic acid detection instrument body 4 through the pipette 505 for detection and analysis. The whole operation process is simple and convenient. Compared with the existing nucleic acid detection instruments, the utility model has a compact design structure and a small overall footprint, which greatly improves the convenience of use and ensures the accuracy and efficiency of the detection results.

[0039] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A detection assembly for nucleic acid detection, comprising a detection box (1), characterized in that: The right end of the detection box (1) is provided with a receiving cavity (2), and a heating component (3) is fixedly installed inside the receiving cavity (2); The heating component (3) comprises a base (301) fixedly mounted inside the accommodating cavity (2); the top of the base (301) is rotatably connected to a rotating table (302); a placement rack (303) is fixedly mounted on the top of the rotating table (302); a plurality of groups of sample test tubes (304) are placed inside the placement rack (303); a heating cover (305) is movably mounted on the top of the base (301) and located outside the placement rack (303); two groups of heating tubes (306) are fixedly mounted on the inner wall of the heating cover (305).

2. A detection component for nucleic acid detection according to claim 1, characterized in that: The left and right sides of the detection box (1) are both provided with transport grooves (101); a sealing cover (102) is rotatably connected to the top of the detection box (1) and located outside the accommodating cavity (2); and a sealing gasket (103) is fixedly connected to the inner side of the sealing cover (102).

3. A detection component for nucleic acid detection according to claim 1, characterized in that: A first motor (307) is fixedly mounted on the right end of the bottom of the base (301); a driving gear (308) is fixedly mounted on the driving end of the first motor (307); a driven gear (309) is rotatably connected to the top of the base (301), and the driven gear (309) is meshed with the driving gear (308); a turntable (310) is fixedly connected to the top of the driven gear (309); a toggle block (311) is fixedly connected to the top of the turntable (310); and a plurality of toggle slots (312) adapted for use with the toggle blocks (311) are provided on the outer side of the rotating platform (302).

4. A detection component for nucleic acid detection according to claim 1, characterized in that: A buckle groove (313) is provided at the center of the top of the heating cover (305); the top of the heating cover (305) is rotatably connected to a movable cover (314); the top of the heating cover (305) and in front of the movable cover (314) is rotatably connected to a first electric telescopic rod (315); and the driving end of the first electric telescopic rod (315) is rotatably connected to the movable cover (314).

5. A nucleic acid analysis device, characterized in that: A detection component for nucleic acid detection according to any one of claims 1 to 4, further comprising: The nucleic acid detector body (4) is fixedly installed inside the left end of the detection box (1), and a pipetting component (5) is fixedly installed on the right end of the top of the nucleic acid detector body (4).

6. A nucleic acid analysis device according to claim 5, characterized in that: An operation display area (401) is provided at the front end of the nucleic acid detection instrument body (4), and a detection port (402) is opened at the left end of the top of the nucleic acid detection instrument body (4).

7. A nucleic acid analysis device according to claim 5, characterized in that: The pipetting assembly (5) includes a fixing seat (501) fixedly mounted on the top of the nucleic acid detection instrument body (4), a second motor (502) is fixedly mounted on the top of the fixing seat (501), a cantilever (503) is fixedly mounted on the driving end of the second motor (502), a second electric telescopic rod (504) is fixedly mounted on the right end of the cantilever (503), and a pipette gun (505) is fixedly mounted on the driving end of the second electric telescopic rod (504).