Culture device suitable for tobacco root system disease resistance identification research

Abstract

The utility model discloses a culture device suitable for tobacco root system disease resistance identification research, and aims to solve the problems that research equipment in the prior art is high in manufacturing cost, and meanwhile, lossless in-situ detection analysis cannot be realized. The utility model provides a culture device suitable for tobacco root system disease resistance identification research. The culture device comprises a transparent culture box, the top of the transparent culture box is provided with a telescopic supporting frame used for straightening tobacco seedlings, the transparent culture box is provided with a stirring mechanism, and the bottom of the transparent culture box is provided with an anti-toppling piece. Through the transparent culture box, the growth length, the root morphology and the growth condition of the roots of the tobacco seedlings can be visually observed. The telescopic supporting frame is arranged to assist in fixing the tobacco seedlings, so that the tobacco seedlings can normally and vertically grow without being affected. By arranging the stirring mechanism, the added protospore liquid is uniformly stirred, the tobacco seedlings fully absorb the protospore liquid, and a more credible identification result can be obtained. The stability of the transparent culture box is enhanced by arranging the anti-toppling piece, and toppling is prevented. The tobacco seedling root in-situ detection device is low in material cost, environment-friendly and reusable, and ensures that the tobacco seedling root is subjected to in-situ detection analysis in a lossless manner.

Description

Technical Field

[0001] The utility model relates to the technical field of tobacco disease resistance identification, in particular to a cultivation device suitable for tobacco root disease resistance identification research. Background Art

[0002] The root system is very important for tobacco plants. First, the root system is an important organ for absorbing water and nutrients. Second, the root system is an important foundation for supporting the upper part. The root systems of different varieties vary greatly. The root morphology itself varies greatly, and there are certain differences in root length, root diameter and root configuration. In addition, there are also great differences between varieties in response characteristics to nutritional stress, biological stress and abiotic stress.

[0003] At present, in tobacco production, rhizome diseases occur to varying degrees in all major tobacco-growing areas, with an average incidence of 10-15%. Some plots are seriously affected, and tobacco plants die on a large scale, causing heavy losses to farmers and seriously dampening their enthusiasm for growing tobacco. Therefore, it is very necessary to cultivate disease-resistant varieties with a high level of resistance to rhizome diseases. Tobacco's resistance to root diseases is also a hot topic of research.

[0004] In the process of breeding disease-resistant varieties, it is very necessary to study the morphological changes of the root system after inoculation, but there is no mature research equipment for in-situ observation and recording of the root system after inoculation. At present, most experiments on inoculation and disease identification use small flower pots to plant tobacco seedlings, inoculate pathogens after a certain seedling age, and judge the disease situation and variety resistance based on the aboveground parts after inoculation, or pull out the roots of the tobacco plants, wash the roots, observe and take pictures. It is difficult to observe the root system without damage, which is not intuitive enough.

[0005] At present, the main instrument for studying the root system is the root scanner. It can accurately study the root system from data such as morphology and root length and number, but due to its relatively high price and the need to dig the tobacco plant roots out of the soil and wash the roots first, it is difficult to perform non-destructive in-situ detection and analysis of the root system. Therefore, it is necessary to invent a relatively low-priced, light and easy to operate device that can directly and timely record the growth and development of the tobacco seedling root system while the plant grows normally and is not affected, record the root system's response to pathogens after inoculation, and the root system's growth and development and morphological change trends and processes. Utility Model Content

[0006] In view of the deficiencies in the above-mentioned background technology, the utility model proposes a cultivation device suitable for tobacco root disease resistance identification research, which solves the problem that the research equipment in the prior art is expensive and cannot achieve non-destructive in-situ detection and analysis.

[0007] The technical solution of the utility model is achieved in this way:

[0008] A cultivation device suitable for tobacco root disease resistance identification research, characterized in that it includes a transparent culture box, a retractable support frame for straightening is provided on the top of the transparent culture box, a stirring mechanism is provided on the transparent culture box, and an anti-dumping piece is provided on the bottom of the transparent culture box. Through the transparent culture box, the growth conditions of the tobacco seedlings such as the growth length, diameter, total number of roots and root system configuration can be observed intuitively. By setting a retractable support frame to assist in fixing the tobacco seedlings, it is ensured that tobacco seedlings at different growth stages and different plant heights can grow normally upright in the culture box without being affected. By setting a stirring mechanism, the fungal spore liquid added when inoculating pathogens to identify disease resistance can be quickly stirred and evenly, and fully absorbed by the tobacco seedlings, so that a more reliable identification result can be obtained. By setting an anti-dumping piece, the stability of the transparent culture box is enhanced to prevent dumping.

[0009] Furthermore, the top surface of the transparent culture box is provided with a culture port and at least one sample addition hole, and a plug cover is detachably provided in the sample addition hole. The culture port provides an entrance for the tobacco seedlings, and the sample addition hole is used to inject pathogenic bacteria spore liquid, which is easy to operate. The plug cover is fixed in the sample addition hole to ensure that the pathogens will not contaminate other tobacco plants, spread pathogens into the environment, and pollute the environment.

[0010] Furthermore, the retractable support frame includes a telescopic rod and a fixed frame, wherein the telescopic rods are two and are arranged oppositely on the top surface of the transparent culture box, and the fixed frame is arranged between the two telescopic rods. The telescopic rod enables the fixed frame to be adjusted to different heights, which is convenient and quick. The fixed frame controls the upright growth of the tobacco seedlings to ensure the normal growth of the tobacco seedlings.

[0011] Furthermore, the fixing frame includes a support rod and an adjustment ring, wherein both ends of the support rod are fixedly connected to the free end of the telescopic rod, and the adjustment ring is arranged in the middle of the support rod and directly opposite to the culture port. The support rod supports the adjustment ring, and the adjustment ring semi-fixes the tobacco seedlings.

[0012] Furthermore, the adjustment ring is a rubber ring that can be tightened or loosened or an annular buckle that can adjust the size. The rubber ring or the annular buckle can play a role in fastening the tobacco seedlings by adjusting different diameters.

[0013] Furthermore, the stirring mechanism includes a small motor and a fan blade, the small motor is arranged on the rear panel of the transparent culture box, and the fan blade is connected to the output shaft of the small motor and extends into the transparent culture box. The small motor controls the rotation of the fan blade to mix the spore liquid evenly in time, so as to facilitate the rapid mixing of the culture liquid after inoculation and the identification of the resistance of tobacco seedlings of different genotypes planted therein to root and rhizome diseases.

[0014] Furthermore, the fan blades are small plastic blades with 3 to 6 leaves, which reduces the damage to the roots of the tobacco seedlings caused by the fan blades during stirring.

[0015] Furthermore, the transparent culture box is a rectangular transparent box body, and a scale grid is provided on the front panel of the rectangular transparent box body. The rectangular transparent box body can visually observe the root system, and the scale grid can conveniently record the root growth at any time and judge the root length.

[0016] Furthermore, the anti-dumping member includes fixing rods arranged on both sides of the bottom of the transparent culture box, the fixing rods are fixedly connected to two side edges of the bottom of the transparent culture box and can extend out of the transparent culture box, so as to enhance the stability of the transparent culture box and prevent it from dumping.

[0017] Furthermore, the anti-dumping member is a bottom plate, which is fixedly connected to the bottom surface of the transparent culture box, and the area of ​​the bottom plate is larger than the bottom surface area of ​​the transparent culture box, thereby ensuring the stability of the transparent culture box.

[0018] The beneficial effects of the utility model are:

[0019] The utility model can visually observe the growth length and growth status of the tobacco seedling root through the transparent culture box, and is convenient for taking photos and recording and retaining image data. The setting of the retractable tobacco seedling support frame can assist in fixing the tobacco seedlings and ensure the normal upright growth of the tobacco seedlings; the setting of the stirring mechanism can timely and evenly mix the pathogen spore liquid when inoculating pathogens to identify disease resistance, which is convenient for more accurate identification and research of tobacco seedling disease resistance; the setting of the anti-dumping part can facilitate the entire device to be placed upright to prevent dumping.

[0020] The material of the utility model is low-cost, environmentally friendly and reusable; it can be used to observe and photograph the root systems of tobacco materials of different varieties (lines) or genotypes themselves; it can study the response of the root system to stress after inoculation with pathogen spore liquid, or under nutritional stress, other biological stress and abiotic stress, and dynamically record the response differences; the utility model can be made into models of different sizes according to needs, and the root systems of tobacco seedlings of different sizes can be studied, and in-situ detection and analysis of the tobacco seedling root system can be guaranteed without damage. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the embodiments of the present utility model, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0022] Figure 1 It is a front view of the utility model;

[0023] Figure 2 It is a left side view of the utility model;

[0024] Figure 3It is a top view of the utility model;

[0025] 1. Transparent culture box; 1-1. Front panel; 1-2. Rear panel; 2. Retractable support frame; 3. Stirring mechanism; 4. Anti-dumping member; 5. Culture port; 6. Sample adding hole; 7. Retractable rod; 8. Fixed frame; 801. Support rod; 802. Adjustment ring; 9. Small motor; 10. Fan blades; 11. Plug cover; 12. Scale grid. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0027] Embodiment 1, as Figure 1 As shown, in this embodiment, a culture device suitable for identification and research of tobacco root disease resistance includes a transparent culture box 1, which is a rectangular transparent box body. A retractable support frame 2 for straightening is provided on the top of the transparent culture box 1. The retractable support frame 2 is a device that can semi-fix and support the tobacco seedlings to stand upright normally. A stirring mechanism 3 is provided on the transparent culture box 1. The stirring mechanism is arranged near the sample addition hole 6. The stirring mechanism is used to stir the added fungal spore liquid evenly to ensure that the tobacco root system can fully and evenly contact the spores of the pathogen, so as to obtain a more reliable identification result. An anti-dumping member 4 is provided at the bottom of the transparent culture box 1 to make the transparent culture box 1 stand stably and prevent it from tipping over.

[0028] On the basis of the above-mentioned embodiment, as a preferred embodiment, the top surface of the transparent culture box 1 is provided with a culture port 5 located in the center and four symmetrically distributed sample addition holes 6, the tobacco seedlings are placed in the transparent culture box 1 through the culture port 5, and the spore liquid of the pathogen is added through the sample addition holes 6. The sample addition holes 6 are detachably provided with a plug cover 11, and after the spore liquid of the pathogen is added and stirred evenly, the sample addition holes are plugged into the sample addition holes 6 with the plug cover 11, and are in a sealed state, so that the pathogens will not contaminate other tobacco plants, will not spread the pathogens into the environment, and will pollute the environment.

[0029] On the basis of the above-mentioned embodiment, as a preferred embodiment, the retractable support frame 2 is a device that can semi-fix and support the tobacco seedlings to stand normally. The retractable support frame 2 includes a telescopic rod 7 and a fixed frame 8. The telescopic rod 7 is two and is relatively arranged on the top surface of the transparent culture box 1, so that the fixed frame 8 can assist in fixing the tobacco seedlings. The fixed frame 8 is arranged between the two telescopic rods 7, so that the telescopic rod 7 can control the height of the fixed rod 8. The telescopic rod 7 is extended and retracted according to the height of the tobacco seedlings to control the height of the fixed frame 8. The fixed frame 8 is assisted in being fixed on the stem of the tobacco seedlings to ensure that the tobacco seedlings grow normally and stand upright.

[0030] Based on the above embodiment, as a preferred embodiment, the fixing frame 8 includes a support rod 801 and an adjustment ring 802. Both ends of the support rod 801 are fixedly connected to the free ends of the telescopic rod 7. The telescopic rod 7 drives the fixing frame 8 to rise or fall. The adjustment ring 802 is arranged in the middle of the support rod 801 and is directly opposite to the position of the culture port 5. The position of the adjustment ring 802 corresponds to the position of the culture port 5 so as to assist in fixing the tobacco seedlings and ensure that the tobacco seedlings grow upright.

[0031] Based on the above implementation, as a preferred implementation, the adjustment ring 802 is a rubber ring that can be tightened or tightened or a ring buckle with adjustable size, and the tobacco seedlings are clamped and fixed by changing the size of the adjustment ring 802; the aperture characteristics of the adjustment ring 802 are based on not affecting the normal growth of the tobacco seedlings.

[0032] On the basis of the above-mentioned embodiment, as a preferred embodiment, the stirring mechanism includes a small motor 9 and a fan blade 10. The small motor 9 is arranged on the upper part of the rear panel 1-2 of the transparent culture box 1 near the sample addition hole 6, and can timely stir and mix the spore liquid added to the sample addition hole 6. The fan blade 10 is connected to the output shaft of the small motor 9 and extends into the transparent culture box 1. The fan blade 10 is a small plastic fan blade with 3 to 6 leaves, so that the fan blade will not cause damage to the tobacco seedlings. The small motor 9 drives the fan blade 10 to rotate, and timely stirs the spore liquid evenly, which is convenient for inoculation and identification of the disease resistance of tobacco seedlings of different genotypes (varieties) planted therein to root and rhizomes diseases.

[0033] On the basis of the above-mentioned embodiment, as a preferred embodiment, the anti-dumping member 4 includes fixing rods arranged on both sides of the bottom of the transparent culture box 1, the fixing rods are fixedly connected to the left and right sides of the bottom of the transparent culture box 1, and the length of the fixing rods can extend out of the transparent culture box 1 to ensure the balance and stability of the transparent culture box 1 and prevent it from dumping.

[0034] Embodiment 2. In this embodiment, the anti-dumping member 4 is a bottom plate, which is fixedly connected to the bottom surface of the transparent culture box 1 and extends out of the bottom surface of the culture box 1. The area of ​​the bottom plate is larger than the bottom surface area of ​​the transparent culture box 1, thereby improving the stability of the culture box 1 and preventing dumping.

[0035] Embodiment 3, as Figure 1 As shown, in this embodiment, a scale grid 12 is provided on the front panel 1-1 of the rectangular transparent box body, and the growth and changes of the roots of the tobacco seedlings can be judged according to the scale grid 12, which is convenient for directly observing the growth status and growth length of the roots of the tobacco seedlings and convenient for taking pictures and recording.

[0036] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A cultivation device suitable for tobacco root disease resistance identification research, characterized in that: The transparent culture box (1) comprises a transparent culture box (1), wherein a retractable support frame (2) for straightening is provided on the top of the transparent culture box (1), a stirring mechanism (3) is provided on the transparent culture box (1), and an anti-dumping member (4) is provided on the bottom of the transparent culture box (1).

2. The cultivation device suitable for tobacco root disease resistance identification research according to claim 1, characterized in that: The top surface of the transparent culture box (1) is provided with a culture port (5) and at least one sample addition hole (6), and a plug cover (11) is detachably provided in the sample addition hole (6).

3. The cultivation device suitable for tobacco root disease resistance identification research according to claim 2, characterized in that: The telescopic support frame (2) comprises a telescopic rod (7) and a fixed frame (8), wherein the telescopic rods (7) are two and are arranged opposite to each other on the top surface of the transparent culture box (1), and the fixed frame (8) is arranged between the two telescopic rods (7).

4. The cultivation device suitable for tobacco root disease resistance identification research according to claim 3, characterized in that: The fixing frame (8) comprises a support rod (801) and an adjustment ring (802), wherein both ends of the support rod (801) are fixedly connected to the free end of the telescopic rod (7), and the adjustment ring (802) is arranged in the middle of the support rod (801) and directly facing the culture port (5).

5. The cultivation device suitable for tobacco root disease resistance identification research according to claim 4, characterized in that: The adjustment ring (802) is a rubber ring that can be tightened or loosened or a ring buckle that can adjust the size.

6. The cultivation device suitable for tobacco root disease resistance identification research according to claim 1, characterized in that: The stirring mechanism comprises a small motor (9) and a fan blade (10); the small motor (9) is arranged on a rear panel (1-2) of the transparent culture box (1); and the fan blade (10) is connected to an output shaft of the small motor (9) and extends into the interior of the transparent culture box (1).

7. The cultivation device suitable for tobacco root disease resistance identification research according to claim 6, characterized in that: The fan blades (10) are small plastic fan blades with 3 to 6 blades.

8. The cultivation device suitable for tobacco root disease resistance identification research according to claim 1, characterized in that: The transparent culture box (1) is a rectangular transparent box body, and a scale grid (12) is provided on the front panel (1-1) of the rectangular transparent box body.

9. The cultivation device suitable for tobacco root disease resistance identification research according to claim 8, characterized in that: The anti-tipping member (4) comprises fixing rods arranged on both sides of the bottom of the transparent culture box (1), the fixing rods being fixedly connected to two side edges of the bottom of the transparent culture box (1) and being able to extend out of the transparent culture box (1).

10. The cultivation device suitable for tobacco root disease resistance identification research according to claim 8, characterized in that: The anti-tipping member (4) is a bottom plate, which is fixedly connected to the bottom surface of the transparent culture box (1), and the area of ​​the bottom plate is larger than the bottom surface area of ​​the transparent culture box (1).

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