Special vessel for freezing and unfreezing embryo

Abstract

The utility model discloses a special embryo freezing and unfreezing vessel which comprises a vessel body, the vessel body is provided with a first groove area, a second groove area and a third groove area, the first groove area is provided with a rectangular groove, and an inverted triangular concave bottom is arranged in the rectangular groove; the second groove area is provided with a plurality of rows of semicircular grooves, and each semicircular groove is in a big-end-up semicircular truncated cone shape; and the loading area is provided with a diagonal plane for placing the freezing loading rod. The special vessel overcomes the problems that in the prior art, when an embryo is frozen or unfrozen, the embryo is inconvenient to position and transfer, liquid is easy to volatilize and the like, and by arranging the rectangular groove with the concave bottom and the semicircular groove with the semicircular truncated cone-shaped structure, the embryo can naturally fall into the bottom of the groove; the semi-circular groove is provided with a half inclined inner wall and can be set to be small and deep, so that the embryo can be quickly positioned, sucked and transferred, the volatilization of liquid is reduced, and the vessel can be repeatedly used in the embryo freezing or unfreezing operation.

Description

Technical Field

[0001] The utility model relates to the technical field of medical equipment, in particular to a special dish for embryo freezing and thawing. Background Art

[0002] Embryo freezing technology is a very important assisted reproductive technology. By quickly freezing embryos cultured in test tubes and preserving them for use when needed, this technology can preserve high-quality embryos and adjust the cycle of embryo transplantation. It is especially important for people whose reproductive cell quality and pregnancy rate may be reduced due to health problems to ensure fertility.

[0003] Before freezing, the embryos need to be placed in the balancing solution and vitrification solution in the freezing dish in turn, and then the embryos are placed on the front end of the freezing rod and placed in liquid nitrogen for freezing. When thawing, a thawing dish is needed for thawing and cleaning. Currently, the commonly used freezing dishes and thawing dishes are four-hole dishes. The four-hole dishes have four cylindrical grooves that can hold freezing solution or thawing solution for freezing or thawing of embryos. However, the cylindrical grooves used have a flat bottom structure that is not convenient for finding the position of the embryos, and the right-angled edges of the grooves are easy to cause obstruction and bump damage to the embryo straw that enters the liquid obliquely when the embryos are transferred. Chinese patent publication number CN212437078U, publication date February 2, 2021, entitled "A special dish for embryo freezing" discloses an embryo freezing dish, the inner bottom surface of the dish body of which is provided with multiple rows of sample adding strips, each sample adding strip includes three sample adding slots connected by a connecting slot, which can be used for batch freezing or thawing operations of embryos. However, the sample adding slot of the freezing dish provided by this patent is still a cylindrical groove, and the above-mentioned problems of inconvenience in finding embryos and damage to embryo straws still exist. In addition, this dish does not have a position for loading embryos. The same circular slot hole is not convenient for distinguishing the type of liquid added, which may lead to misoperation. In order to avoid further increasing the difficulty of finding embryos, the sample adding slot is generally set shallower, and in order to ensure sufficient liquid, a larger notch is required, which will aggravate the volatilization of liquid components and cause changes in liquid concentration. Usually, covering with paraffin oil is used to reduce liquid volatilization, but in actual operation, a few embryos will be contaminated with paraffin oil particles, which are difficult to remove during the thawing process, which may affect the quality of the embryos. Utility Model Content

[0004] The utility model overcomes the problems of the prior art such as inconvenient embryo positioning during embryo freezing or thawing, inconvenient operation during embryo transfer, and easy volatility of liquid in the groove, and provides a special dish for embryo freezing and thawing. By arranging a rectangular groove with a concave bottom and a semicircular groove with a semi-truncated cone structure, the embryo will naturally fall into a specific position at the bottom of the groove. The semicircular groove has a half-oblique inner wall and can be set small and deep, so that rapid positioning and suction transfer of the embryo can be achieved, and the volatilization of the liquid is reduced. The dish can be circulated and reused in embryo freezing or thawing operations.

[0005] In order to achieve the above purpose, the utility model adopts the following scheme:

[0006] The special dish for embryo freezing and thawing comprises a dish body, on which are arranged:

[0007] A first groove area is provided with a rectangular groove, wherein an inverted triangular concave bottom is provided in the rectangular groove;

[0008] The second groove area is provided with a plurality of rows of semicircular grooves, wherein the shape of the semicircular grooves is a semi-truncated cone with a larger top and a smaller bottom;

[0009] A loading area is provided with a chamfered surface for placing frozen carrier rods.

[0010] Preferably, it also includes a cover plate matched with the dish body.

[0011] Preferably, the loading area, the first slot area and the second slot area are arranged in sequence from left to right.

[0012] Preferably, the lowest positions in the rectangular groove and the semicircular groove are both on the left.

[0013] Preferably, the chamfered surface of the loading area gradually deepens from left to right.

[0014] Preferably, the rectangular groove has a length of 15mm-20mm, a width of 8mm-10mm, and a maximum depth of 4mm-6mm; the semicircular groove has a groove opening diameter of 4mm-6mm, a groove bottom diameter of 2mm-4mm, and a depth of 7mm-9mm.

[0015] The utility model has at least the following beneficial effects: (1) the rectangular groove adopts a concave bottom structure, and the semicircular groove adopts a semi-truncated cone structure with a larger top and a smaller bottom, which can realize rapid positioning of embryos and improve the efficiency of embryo transfer and freezing operations; (2) the semicircular groove has a partially inclined inner wall, which is convenient for obliquely inserting the embryo suction straw into the groove, which meets the requirements of the conventional method of manual operation, makes the operation smoother and avoids the damage of the suction tool by the groove; (3) the rectangular groove can be designed to be large and shallow, and the semicircular groove can be designed to be small and deep. A rectangular groove and multiple rows of semicircular grooves can be combined to realize batch operation of embryos. The semicircular grooves can be arranged more closely and occupy less space. The dish body can be designed to be more compact, and the grooves of different shapes make it easy to distinguish the functions of the grooves on the dish and the liquid contained therein, avoiding misoperation; (4) The semi-truncated cone structure inside the semicircular groove can reduce the surface area of ​​the liquid while ensuring the amount of liquid, thereby reducing the volatilization caused by the contact between the liquid and the air. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a top view of the overall structure of the utility model;

[0017] Figure 2 The utility model is a schematic diagram of a slot structure.

[0018] In the figure: dish body 1, rectangular groove 2, semicircular groove 3, chamfered surface 4, cover plate 5. DETAILED DESCRIPTION

[0019] The present invention will be further described in detail below in conjunction with the accompanying drawings so that those skilled in the art can implement the invention with reference to the description.

[0020] It should be understood that the terms such as “having”, “including” and “comprising” used herein do not exclude the existence or addition of one or more other elements or combinations thereof.

[0021] It should be noted that the experimental methods described in the following embodiments are conventional methods unless otherwise specified, and the materials can be obtained from commercial channels unless otherwise specified; in the description of the present utility model, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "set" should be understood in a broad sense, for example, they can be fixedly connected, set, or detachably connected, set, or connected and set in one piece. For ordinary technicians in this field, the specific meanings of the above terms in the present utility model can be understood in specific circumstances. The orientation or position relationship indicated by the terms "transverse", "longitudinal", "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc. is based on the orientation or position relationship shown in the accompanying drawings, which is only for the convenience of describing the present utility model and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation to the present utility model.

[0022] like Figure 1 and Figure 2 As shown, the embryo freezing and thawing special dish provided by the utility model comprises a dish body 1, on which the following are arranged:

[0023] The first tank area is provided with a rectangular groove 2, and an inverted triangle concave bottom is provided in the rectangular groove 2; the rectangular groove 2 is generally larger and shallow, and the lowest point of the concave bottom of the inverted triangle is a bottom line, and the bottom line is along the length direction of the rectangular groove 2 and can be marked with thinner colored lines, and the embryo in the groove will fall on this bottom line when stationary, and the specific position of the embryo can be quickly found along this bottom line, and the bottom line is at a certain distance from the sidewall of the rectangular groove 2, so that the embryo straw is convenient to approach the bottom line at an inclined angle and will not touch the notch edge of the rectangular groove 2. When carrying out embryo freezing operation, the rectangular groove 2 is used to hold balancing liquid, and a plurality of embryos to be frozen can be accommodated simultaneously as required, and balancing liquid is used to stabilize the internal and external osmotic pressure of the embryo, so that the embryonic cells can adapt to the change of osmotic pressure when being subsequently placed in the vitrification freezing liquid to prevent being damaged, and the balancing liquid usually contains specific ions, nutrients and protective agents, and these compositions help to maintain the physiological state of the embryo. When thawing, the rectangular groove 2 is used to hold thawing liquid, and thawing liquid provides a suitable environment for the thawing of the embryo and the recovery of normal physiological function.

[0024] The second groove area is provided with a plurality of rows of semicircular grooves 3, and the shape of the semicircular grooves 3 is a semi-truncated cone with a larger upper part and a smaller lower part; one side of the semi-truncated cone-shaped groove body is inclined downward, and when sucking and transferring embryo cells, the embryo suction pipette extends obliquely into the groove hole from this side, and even if the groove hole is set small and deep, the embryo suction pipette is not easily obstructed or bumped by the edge of the groove, because in actual operation, the embryo suction pipette only needs to be operated on the inclined side, so the semi-truncated cone-shaped groove hole will not affect the normal operation, and the inclined embryo suction pipette will not block the line of sight during operation, and the area of ​​the semicircular groove is only half of the area of ​​the full circular groove with the same diameter, which can effectively reduce the exposed area of ​​the liquid in the groove to reduce the volatilization of the liquid component. After standing, the embryo will automatically slide down along the inclined surface onto the smaller plane of the groove bottom, and the position of the embryo can be quickly found when the groove hole is set deeper, so a deeper and smaller groove hole size can be set, and the groove can hold a sufficient amount of liquid while reducing volatilization, maintaining the liquid concentration and the proportion of each component. When performing embryo freezing operation, vitrification freezing liquid is placed in the semicircular groove 3, and the vitrification freezing liquid contains cryoprotectants, such as dimethyl sulfoxide or glycerol, and other substances that can protect cells from low temperature damage. The protective agent in the freezing liquid can penetrate into the cell, reduce the possibility of ice crystals forming in the cell during freezing, thereby protecting the embryo from damage, and the embryo is transferred from the rectangular groove 2 to the semicircular groove 3 and fully soaked before loading and freezing operation. When performing embryo thawing, diluent and cleaning liquid are placed in the semicircular groove 3, a group of diluent and cleaning liquid are proportioned according to the material concentration gradient and placed in a row of semicircular grooves 3, the number of single-row semicircular grooves 3 can have multiple configuration specifications, and the number of diluents and cleaning liquids is selected and used according to actual needs, and the embryo is transferred from the thawing liquid of the rectangular groove 2 and soaked in the diluent and cleaning liquid in turn, and the freezing liquid remaining in the embryo is fully analyzed and the embryo osmotic pressure is balanced and placed in the culture medium for embryo culture.

[0025] The loading area is provided with an oblique surface 4 for placing a freezing rod. The oblique surface 4 of the loading area extends obliquely downward from the surface of the dish body 1 and is used when freezing embryos to obliquely place the front end of the freezing rod, so as to facilitate accurate loading of the embryos on the freezing rod.

[0026] When using this dish for embryo freezing, add the balancing solution into the rectangular groove 2, put all the embryos to be frozen of the same patient into the rectangular groove 2, wait for 5-20 minutes, then transfer the embryos into the semicircular groove 3 by sucking with a Pasteur pipette, and put each embryo into a semicircular groove 3 separately, into which the vitrification freezing solution is added in advance, and the position of the embryo in the vitrification freezing solution is moved several times to fully exchange the internal and external substances, and after 45-60 seconds, the embryo is sucked and loaded onto the front end of the freezing rod placed in the loading area in advance, and the freezing rod is placed in liquid nitrogen for cryopreservation.

[0027] When using this dish to thaw embryos, the thawing solution is heated to 37 degrees Celsius and added to the rectangular groove 2, the diluent and cleaning solution at room temperature are added to different slots in a row of semicircular grooves 3, respectively, the embryos to be thawed are placed in the thawing solution in the rectangular groove 2, the embryos fall off the freezing support rod and are heated and thawed for 1 minute, then the embryos are transferred to the diluent and kept for 3 minutes to reduce the concentration of the cryoprotectant in the embryos, and then the embryos are transferred to the cleaning solution for cleaning to remove the residual freezing solution components, and the cleaning can be performed twice or more depending on the situation. The embryos are soaked in the cleaning solution for 5 minutes each time, and finally the embryos are placed in the culture solution for culture for use.

[0028] It should be noted that the time ranges mentioned above are examples of reference values. The actual operation steps and specific times can be adaptively adjusted according to the instructions of the special liquid kit for embryo freezing and thawing used.

[0029] The special dish for embryo freezing and thawing can be used in embryo freezing or thawing operations. The rectangular groove 2 adopts a concave bottom structure, and the semicircular groove 3 adopts a semi-truncated cone structure with a large top and a small bottom, which can realize rapid positioning of the embryo and improve the efficiency of embryo transfer and freezing operations; the semicircular groove 3 has a partially inclined inner wall, which is convenient for obliquely inserting the embryo suction tube into the groove, which meets the requirements of the conventional manual operation method, makes the operation smoother and avoids the damage of the suction tube tool by collision with the groove; the rectangular groove 2 can be designed to be large and shallow, and the semicircular groove 3 can be designed to be small and deep, and one rectangular groove 2 and multiple rows of semicircular grooves can be designed to be small and deep. The semicircular grooves 3 can be used together to realize batch operation of embryos. The lateral space occupied by the semicircular grooves 3 is half of that of the full circular grooves. Due to the operation mode of holding the straw with one hand, the embryo transfer will not be affected. The arrangement of the semicircular grooves 3 is more compact, occupying less space, making the dish body 1 more compact, and the grooves of different shapes are convenient for distinguishing the functions of the groove holes on the dish and the liquid contained therein, thereby avoiding misoperation. The semi-conical structure inside the semicircular grooves 3 has small and deep groove holes, which can reduce the area of ​​liquid contacting the air to half of the circular hole while ensuring the amount of liquid, thereby reducing the volatilization of the liquid.

[0030] In another technical solution, Figure 2 As shown, it also includes a cover plate 5 that cooperates with the dish body 1. The cover plate 5 can be a rectangular plate, which is arranged in close contact with the upper surface of the dish body 1 and can at least cover all the semicircular grooves 3 in the second groove area, so as to prevent the solution from being contaminated and further reduce the volatilization of the solution.

[0031] In another technical solution, Figure 1 and Figure 2 As shown, the loading area, the first tank area and the second tank area are arranged from left to right. The loading area is arranged on the far left, which is convenient for holding the freezing carrier rod with the left hand and placing it in a suitable position when loading embryos. Each area is clearly divided, and the first tank area and the second tank area are arranged adjacent to each other on the left and right to facilitate the transfer of embryos.

[0032] The lowest positions in the rectangular groove 2 and the semicircular groove 3 are both on the left. Since most people are accustomed to holding the embryo pipette with their right hand to perform embryo transfer operations, the lowest position in the groove is offset to the left to provide more tilting space for the pipette to avoid obstruction of vision when sucking embryos.

[0033] The chamfered surface 4 of the loading area gradually deepens from left to right. The chamfered surface 4 is higher on the left and lower on the right, with an inclination angle of 15-35 degrees, which better meets the inclination requirements of the load bar during loading.

[0034] In another technical solution, the rectangular groove 2 has a length of 15mm-20mm, a width of 8mm-10mm, and a maximum depth of 4mm-6mm; the semicircular groove 3 has a groove opening diameter of 4mm-6mm, a groove bottom diameter of 2mm-4mm, and a depth of 7mm-9mm. This size range is the preferred range obtained by summarizing and adjusting the existing products and actual use. As an example, the height of the selected dish body 1 is 10mm, the rectangular groove 2 is 20mm long, 10mm wide, 5mm deep at the maximum, and the height of the inverted triangle concave bottom is 2mm, the length direction of the rectangular groove 2 is the width direction of the dish body 1, the notch diameter of the semicircular groove 3 is 5mm, the bottom diameter of the groove is 3mm, and the depth is 8mm. The semicircular groove 3 is arranged in 3 rows, and the number of slot holes in each row is 3. The number of rows and the number of slot holes in each row can be expanded as needed. The length and width of the dish body 1 are adaptively adjusted according to the distribution of the rectangular groove 2, the semicircular groove 3 and the chamfered surface 4 of the loading area. Each area on the dish has a certain margin from the edge of the dish body 1, and the corners on the dish body 1 can be treated with an arc transition.

[0035] The number of devices and processing scales described here are used to simplify the description of the utility model. Applications, modifications and variations of the utility model are obvious to those skilled in the art.

[0036] Although the implementation scheme of the utility model has been disclosed as above, it is not limited to the applications listed in the specification and implementation modes. It can be fully applied to various fields suitable for the utility model. For those familiar with the art, additional modifications can be easily implemented. Therefore, without departing from the general concept defined by the claims and the scope of equivalents, the utility model is not limited to the specific details and the illustrations shown and described herein.

Claims

1. A special dish for embryo freezing and thawing, characterized in that: It comprises a dish body, on which is arranged: A first groove area is provided with a rectangular groove, wherein an inverted triangular concave bottom is provided in the rectangular groove; The second groove area is provided with a plurality of rows of semicircular grooves, wherein the shape of the semicircular grooves is a semi-truncated cone with a larger top and a smaller bottom; A loading area is provided with a chamfered surface for placing frozen carrier rods.

2. The embryo freezing and thawing dish according to claim 1, characterized in that: Also included is a cover plate matched with the dish body.

3. The embryo freezing and thawing dish according to claim 1, characterized in that: The loading area, the first slot area and the second slot area are arranged in sequence from left to right.

4. The embryo freezing and thawing dish according to claim 3, characterized in that: The lowest positions in the rectangular groove and the semicircular groove are both on the left.

5. The embryo freezing and thawing dish according to claim 3, characterized in that: The chamfered surface of the loading area gradually deepens from left to right.

6. The embryo freezing and thawing special dish according to claim 1, characterized in that: The rectangular groove has a length of 15mm-20mm, a width of 8mm-10mm, and a maximum depth of 4mm-6mm; the semicircular groove has a groove opening diameter of 4mm-6mm, a groove bottom diameter of 2mm-4mm, and a depth of 7mm-9mm.

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