Solid state fermentation bag

Abstract

The utility model provides a solid state fermentation bag which comprises a bag body (1) with an opening (2), a sealing strip (3) is arranged on the inner side of the bag body and can seal the opening and form a sealed space in the bag body, a diaphragm (4) is transversely arranged in the bag body and divides the sealed space into an upper cavity (5) and a lower cavity (6), and a plurality of one-way air inlet valves (7) are embedded in the diaphragm at intervals. A one-way exhaust valve (8) is embedded in the bag body of the upper cavity, and an air inlet is formed in the bag body of the lower cavity and used for being communicated with an air source (13). When the solid-state fermentation bag is used for aerobic fermentation, a stable airflow channel is formed in the bag body, oxygen can be in full contact with fermentation raw materials, and it is ensured that aerobic fermentation is continuously and stably carried out. The solid-state fermentation bag can realize high-efficiency switching between anaerobic fermentation and aerobic fermentation, fermentation equipment does not need to be switched, and the risk of strain pollution is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of fermentation, in particular to a solid-state fermentation bag. Background Art

[0002] Solid-state fermentation is a technology that uses solid substrates to ferment and grow microorganisms under certain temperature, humidity, and oxygen conditions. It can produce useful products such as antibiotics, proteins, enzymes, organic acids, and biofuels, and is widely used in the fields of medicine, food, textiles, and biofuels. Solid-state fermentation is divided into two types according to fermentation conditions: aerobic fermentation and anaerobic fermentation. Aerobic fermentation is generally carried out in equipment such as fermentation tanks, while anaerobic fermentation is generally carried out in sealed fermentation bags or cellars.

[0003] Existing fermentation equipment cannot have both aerobic and anaerobic fermentation functions. For example, fermentation bags can only perform anaerobic fermentation but not aerobic fermentation. When using fermentation bags for aerobic fermentation, due to the poor gas circulation in the fermentation bags, the oxygen introduced into the fermentation bags cannot fully contact the fermentation raw materials, and it is impossible to cause the oxygen to contact and mix with the fermentation raw materials by stirring, etc., which makes aerobic fermentation difficult. When performing solid-state fermentation, in order to achieve better fermentation effects, it is usually necessary to frequently switch or alternate between aerobic fermentation and anaerobic fermentation. When switching between aerobic fermentation and anaerobic fermentation, the fermentation equipment must be switched. Switching the fermentation equipment is not only cumbersome to operate, but also increases the risk of bacterial contamination.

[0004] Therefore, there is an urgent need for a fermentation bag that has both aerobic fermentation and anaerobic fermentation functions to solve the problem of cumbersome operation and increased risk of bacterial contamination caused by switching fermentation equipment in the prior art. Utility Model Content

[0005] The utility model aims to overcome the problems in the prior art of complicated operation and increased risk of bacterial contamination when switching between aerobic fermentation and anaerobic fermentation, and to provide a solid fermentation bag, which has both aerobic fermentation and anaerobic fermentation functions, can switch between aerobic fermentation and anaerobic fermentation as needed, and the switching process is simple and easy to operate and is not prone to bacterial contamination.

[0006] In order to achieve the above-mentioned purpose, the utility model provides a solid fermentation bag, including a bag body with an opening, a sealing strip is arranged on the inner side of the bag body, the sealing strip can seal the opening and form a sealed space inside the bag body, a diaphragm is arranged horizontally in the bag body, the diaphragm divides the sealed space into an upper chamber and a lower chamber, a plurality of one-way air inlet valves that only allow gas to enter the upper chamber from the lower chamber are embedded on the diaphragm at intervals, a one-way exhaust valve is embedded on the bag body of the upper chamber, and an air inlet is arranged on the bag body of the lower chamber, and the air inlet is used to connect to a gas source.

[0007] Preferably, the plurality of one-way air inlet valves are evenly distributed on the diaphragm.

[0008] Preferably, the one-way air intake valve is a plastic one-way valve; and / or, the one-way air exhaust valve is a plastic one-way valve.

[0009] Preferably, the air inlet is connected to the air source through an air inlet pipe.

[0010] Preferably, a flow control valve is provided on the air intake pipe; and / or a power pump is provided on the air intake pipe.

[0011] Preferably, the outlet end of the one-way exhaust valve is connected to the gas collecting device through an exhaust pipe.

[0012] Preferably, a heat-seal identification line is provided on the bag body above the sealing strip.

[0013] Preferably, a filling line is provided on the bag body of the upper chamber, the space below the filling line of the upper chamber is used to fill the fermentation raw materials, and the space above the filling line of the upper chamber is used to collect the gas produced during the fermentation process.

[0014] Preferably, the one-way exhaust valve is arranged on the bag body above the filling line.

[0015] Preferably, the volume ratio of the upper chamber to the lower chamber exceeds 5:1.

[0016] The utility model has the following beneficial effects:

[0017] (1) The solid-state fermentation bag can achieve efficient aerobic fermentation. The oxygen-containing gas is introduced into the lower chamber through the gas source, and the oxygen-containing gas is introduced into multiple parts of the upper chamber through multiple one-way air inlet valves embedded in the diaphragm at intervals, thereby ensuring that the oxygen-containing gas can be introduced into the upper chamber more evenly and fully contact and mix with the fermentation raw materials in the upper chamber, and then aerobic fermentation is carried out. The gas produced by aerobic fermentation is automatically discharged through the one-way exhaust valve. Therefore, when the solid-state fermentation bag is used for aerobic fermentation, a stable gas flow path along the lower chamber-one-way air inlet valve-fermentation raw materials-one-way exhaust valve is formed in the bag body, and oxygen can fully contact with the fermentation raw materials, ensuring that aerobic fermentation is carried out continuously and stably.

[0018] (2) The solid-state fermentation bag not only has aerobic fermentation function, but also has the anaerobic fermentation function of traditional fermentation bags. If anaerobic fermentation is required, the gas source can be turned off, or inert gas such as carbon dioxide can be filled into the bag through the gas source according to process requirements to make the bag body in an anaerobic state, and the one-way exhaust valve automatically exhausts the air. If aerobic fermentation is required, the gas source can be turned on, and sterile air or sterile oxygen can be filled into the bag body. At the same time, the one-way exhaust valve automatically exhausts the air to maintain a stable airflow channel to ensure that aerobic fermentation is continuous and stable. Therefore, the solid-state fermentation bag can achieve efficient switching between anaerobic fermentation and aerobic fermentation without switching fermentation equipment, thereby reducing the risk of bacterial contamination. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 is a schematic structural diagram of a solid-state fermentation bag in one embodiment;

[0020] Figure 2 is a schematic structural diagram of a diaphragm in an embodiment;

[0021] The reference numerals are described as follows:

[0022] 1-bag body; 2-opening; 3-sealing strip; 4-diaphragm; 5-upper chamber; 6-lower chamber; 7-one-way air inlet valve; 8-one-way exhaust valve; 9-inlet pipe; 10-exhaust pipe; 11-heat-seal identification line; 12-filling line; 13-air source. DETAILED DESCRIPTION

[0023] The specific implementation of the present invention is described in detail below in conjunction with the accompanying drawings. It should be understood that the specific implementation described here is only used to illustrate and explain the present invention, and is not used to limit the present invention.

[0024] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "set", "install", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a direct connection, or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0025] In the description of the present invention, unless otherwise stated, the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside", etc. indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings, or the directions or positional relationships in which the product of the application is usually placed when in use. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as a limitation on the present invention.

[0026] In addition, if there are descriptions involving "first", "second", etc. in the utility model, the descriptions of "first", "second", etc. are only for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" or "second" may explicitly or implicitly include at least one of the features.

[0027] Reference Figure 1-Figure 2A solid-state fermentation bag provided in one embodiment includes a bag body 1 with an opening 2, a sealing strip 3 is arranged on the inner side of the bag body 1, the sealing strip 3 can seal the opening 2 and form a sealed space inside the bag body 1, a diaphragm 4 is arranged horizontally in the bag body 1, the diaphragm 4 divides the sealed space into an upper chamber 5 and a lower chamber 6, a plurality of one-way air inlet valves 7 that only allow gas to enter the upper chamber 5 from the lower chamber 6 are embedded at intervals on the diaphragm 4, a one-way exhaust valve 8 is embedded on the bag body 1 of the upper chamber 5, and an air inlet is arranged on the bag body 1 of the lower chamber 6, and the air inlet is used to connect to a gas source 13.

[0028] The solid-state fermentation bag not only retains the anaerobic fermentation function of the traditional fermentation bag, but also has the aerobic fermentation function. When aerobic fermentation is performed, oxygen-containing gas is introduced into the lower chamber 6 through the gas source 13, and the oxygen-containing gas is introduced into multiple parts of the upper chamber 5 through multiple one-way air inlet valves 7 embedded in the diaphragm 4. The multiple parts are distributed at intervals along the radial direction of the bag body 1, thereby ensuring that the oxygen-containing gas can be uniformly introduced into the upper chamber 5 and fully contact and mix with the fermentation raw materials in the upper chamber 5, and then aerobic fermentation is performed, and the gas produced by aerobic fermentation is automatically discharged through the one-way exhaust valve 8. Therefore, when fermentation is performed using the solid-state fermentation bag, a stable gas flow path along the lower chamber 6-one-way air inlet valve 7-fermentation raw materials-one-way exhaust valve 8 is formed in the bag body 1, and oxygen can fully contact with the fermentation raw materials, ensuring that aerobic fermentation is carried out continuously and stably. The solid-state fermentation bag can achieve high-efficiency switching between anaerobic fermentation and aerobic fermentation, without switching the fermentation equipment, and reducing the risk of bacterial contamination.

[0029] The method of using the solid-state fermentation bag is as follows: after the fermentation raw materials and the fermentation strains are mixed evenly, they are added to the bag body 1, the bag body 1 is sealed by the sealing strip 3, the bag body 1 is placed upright or flat for stacking, and anaerobic fermentation or aerobic fermentation is performed under certain temperature, light and other conditions. During the fermentation process, the sealing strip 3 can be opened to add other auxiliary materials according to the process requirements. If anaerobic fermentation is required, the gas source 13 can be closed, or an inert gas such as carbon dioxide can be filled into the gas source 13 according to the process requirements, so that the bag body 1 is in an anaerobic state, and the one-way exhaust valve 8 is automatically exhausted. If aerobic fermentation is required, the gas source 13 can be opened, and sterile air or sterile oxygen can be filled into the bag body 1, and the one-way exhaust valve 8 is automatically exhausted to maintain a stable airflow channel to ensure that aerobic fermentation is continuous and stable. During the entire fermentation process, anaerobic fermentation and aerobic fermentation can be switched as needed. After the fermentation is completed, the bag body 1 can be sealed again using heat sealing technology.

[0030] In one embodiment, the bag body 1 is made of plastic. Further, the diaphragm 4 and the bag body 1 are an integral structure, and the material of the diaphragm 4 is the same as that of the bag body 1.

[0031] Continue to refer to Figure 2In one embodiment, a plurality of one-way air inlet valves 7 are arranged at intervals on the diaphragm 4. Further, the plurality of one-way air inlet valves 7 are evenly distributed on the diaphragm 4, and the intervals between two adjacent one-way air inlet valves 7 are equal, so that the oxygen-containing gas can be more evenly introduced into the upper chamber 5 and fully contact and mix with the fermentation raw materials in the upper chamber 5. The oxygen-containing gas is sterile air or sterile oxygen. The number of one-way air inlet valves 7 can be set as needed. For example, 7 one-way air inlet valves 7 are provided.

[0032] In one embodiment, the one-way air inlet valve 7 is a plastic one-way valve; and / or the one-way exhaust valve 8 is a plastic one-way valve. The plastic one-way valve can be welded or glued to the bag body 1 by hot pressing with a valve pressing machine, and has a simple structure and is easy to install.

[0033] In one embodiment, one or more one-way exhaust valves 8 may be provided as required. Furthermore, the number and flow parameters of the one-way air inlet valves 7 and the one-way exhaust valves 8 may be selected so that the flow rates of all the one-way air inlet valves 7 are equal to the flow rates of all the one-way exhaust valves 8, thereby maintaining the air pressure in the bag body 1 stable.

[0034] In one embodiment, the air inlet is connected to the air source 13 through the air inlet pipe 9. Further, a flow control valve is provided on the air inlet pipe 9 to adjust the flow of the oxygen-containing gas introduced through the flow control valve. A filter membrane that can isolate bacteria is provided in the flow control valve to reduce the bacterial content in the oxygen-containing gas. Further, a power pump is provided on the air inlet pipe 9, and the power pump is used to provide power for the oxygen-containing gas to ensure the formation of a stable gas flow path, thereby ensuring that aerobic fermentation is carried out continuously and stably.

[0035] In one embodiment, one end of the air inlet pipe 9 is disposed on the side wall of the lower chamber 6 to prevent the air inlet pipe 9 from being squeezed when the bags 1 are stacked upright. However, one end of the air inlet pipe 9 can also be disposed at the bottom of the lower chamber 6 as needed to prevent the air inlet pipe 9 from being squeezed when the bags 1 are stacked horizontally.

[0036] In one embodiment, the outlet end of the one-way exhaust valve 8 is connected to a gas collecting device through an exhaust pipe 10. The gas produced in the upper cavity is recovered by the gas collecting device to promote the reuse of the produced gas and prevent gas pollution. Furthermore, a ball valve is provided on the exhaust pipe 10, and the ball valve can be used to control the on-off of the exhaust pipe 10. Furthermore, a vacuum pump can be provided on the exhaust pipe 10, and the vacuum pump is used to generate a negative pressure in the exhaust pipe 10, so as to use the negative pressure to completely discharge the produced gas in the bag body 1. The air inlet pipe 9 and the exhaust pipe 10 are preferably hoses or hard pipes with a certain pressure bearing capacity to ensure that the pipelines remain unobstructed when the solid-state fermentation bags are stacked.

[0037] In one embodiment, a heat seal identification line 11 is provided on the bag body 1 above the sealing strip 3. A heat sealer is used to perform heat sealing along the heat seal identification line 11 to form a secondary seal in addition to the sealing of the sealing strip 3, ensuring that the finished fermented product will not be spread out due to operations such as movement and transportation, resulting in the opening 2 of the fermentation bag. If no other substances are added during the fermentation process, the heat seal treatment can be performed at the initial stage of fermentation.

[0038] In one embodiment, a filling line 12 is provided on the bag body 1 of the upper chamber 5, and the space below the filling line 12 of the upper chamber 5 is used to fill the fermentation raw materials, and the space above the filling line 12 of the upper chamber 5 is used to collect the gas produced during the fermentation process. When filling, the filling line 12 is used to indicate the injection amount of the fermentation raw materials, ensuring that the upper part of the upper chamber can reserve space for gathering and collecting the gas production, and the gas production can form a gas phase interval, and the gas phase interval forms air pressure. Driven by the air pressure, the gas production can be smoothly discharged from the fermentation bag through the one-way exhaust valve 8. Furthermore, the one-way exhaust valve 8 is provided on the bag body 1 above the filling line 12 to prevent the extrusion of the fermentation raw materials from affecting the normal exhaust of the one-way exhaust valve 8, so that the gas production can be smoothly discharged through the one-way exhaust valve 8.

[0039] The upper chamber 5 is the region where the fermentation reaction occurs. To improve the utilization rate of the bag body 1, the upper chamber 5 should have a larger volume than the lower chamber 6. In one embodiment, the volume ratio of the upper chamber 5 to the lower chamber 6 exceeds 5:1.

[0040] The preferred embodiments of the present invention are described in detail above in conjunction with the accompanying drawings, but the present invention is not limited thereto. Within the technical concept of the present invention, the technical solution of the present invention can be subjected to a variety of simple modifications, including the combination of various specific technical features in any suitable manner. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations. However, these simple modifications and combinations should also be regarded as the contents disclosed by the present invention and belong to the protection scope of the present invention.

Claims

1. A solid-state fermentation bag, comprising a bag body (1) having an opening (2), wherein a sealing strip (3) is arranged on the inner side of the bag body (1), wherein the sealing strip (3) can seal the opening (2) and form a sealed space inside the bag body (1), characterized in that: A diaphragm (4) is transversely arranged in the bag body (1), and the diaphragm (4) divides the sealed space into an upper chamber (5) and a lower chamber (6). A plurality of one-way air inlet valves (7) are embedded at intervals on the diaphragm (4) to only allow gas to enter the upper chamber (5) from the lower chamber (6). A one-way exhaust valve (8) is embedded on the bag body (1) of the upper chamber (5), and an air inlet is arranged on the bag body (1) of the lower chamber (6), and the air inlet is used to connect to a gas source (13).

2. The solid-state fermentation bag according to claim 1, characterized in that: The plurality of one-way air inlet valves (7) are evenly distributed on the diaphragm (4).

3. The solid-state fermentation bag according to claim 1, characterized in that: The one-way air intake valve (7) is a plastic one-way valve; and / or the one-way air exhaust valve (8) is a plastic one-way valve.

4. The solid-state fermentation bag according to claim 1, characterized in that: The air inlet is connected to the air source (13) via an air inlet pipe (9).

5. The solid-state fermentation bag according to claim 4, characterized in that: The air intake pipe (9) is provided with a flow control valve; and / or the air intake pipe (9) is provided with a power pump.

6. The solid-state fermentation bag according to claim 1, characterized in that: The outlet end of the one-way exhaust valve (8) is connected to a gas collecting device via an exhaust pipe (10).

7. The solid-state fermentation bag according to claim 1, characterized in that: A heat-sealing identification line (11) is provided on the bag body (1) above the sealing strip (3).

8. The solid-state fermentation bag according to claim 1, characterized in that: A filling line (12) is provided on the bag body (1) of the upper chamber (5); the space of the upper chamber (5) below the filling line (12) is used to fill fermentation raw materials; and the space of the upper chamber (5) above the filling line (12) is used to collect gas produced during the fermentation process.

9. The solid-state fermentation bag according to claim 8, characterized in that: The one-way exhaust valve (8) is arranged on the bag body (1) above the filling line (12).

10. The solid-state fermentation bag according to any one of claims 1 to 9, characterized in that: The volume ratio of the upper chamber (5) to the lower chamber (6) exceeds 5:1.

Images