Cell counting plate

Abstract

The utility model relates to the field of biology, and discloses a cell counting plate which comprises a counting plate body, the lower surface of the counting plate body is provided with a catching groove, the upper surface of the counting plate body is connected with a lower counting plate in an inserted mode, and the upper surface of the counting plate body is connected with an upper counting plate in an inserted mode. The upper surface of the upper counting plate penetrates through and is fixedly connected with an observation window, the upper surface of the lower counting plate is provided with a disassembling assembly, the upper surface of the lower counting plate is provided with a sealing assembly, the upper surface of the upper counting plate is provided with a feeding assembly, the disassembling assembly comprises a clamping groove, and a clamping block is inserted into the inner wall of the clamping groove. According to the cell counting plate, the upper counting plate body and the lower counting plate body can be flexibly split through cooperation of the splitting assembly, cell sap in the upper counting plate body and the lower counting plate body can be conveniently cleaned, and therefore the cleaning efficiency of the counting plates is improved, cross contamination caused by cell residues is effectively reduced, and the accuracy and reliability of a follow-up cell counting experiment are guaranteed.

Description

Technical Field

[0001] The utility model relates to the field of biology, in particular to a cell counting plate. Background Art

[0002] The cell counting chamber is a widely used tool in biological and medical research for quantitative analysis of cells. In the early days of cell biology research, accurate determination of cell number was a huge challenge. Scientists need a reliable and easy way to count cells in order to understand physiological processes such as cell growth, division, and death.

[0003] The invention of the microscope provided the basis for cell counting, but it was difficult to accurately quantify the number of cells by simply observing the cells directly through a microscope. Thus, the cell counting plate came into being.

[0004] After searching, Chinese patent announcement number: CN218202825U discloses a cell counting plate for convenient counting, including a fixed plate, a fixing groove is opened on the top surface of the fixed plate, and handles are fixedly installed at both ends of the fixed plate; a sealing component, the sealing component is arranged inside the fixing groove, and the counting device is sealed by setting the fixing groove. The sealing component and the counting device can be fixed through the fixing groove, so that the counting device is more convenient when injecting and taking out the culture solution, and the problem of deviation causing leakage of the culture solution will not occur. By setting a sealing sleeve, the gap between the lower counting plate and the upper counting plate that are directly fixedly installed can be sealed by the sealing sleeve, which can effectively prevent microorganisms from entering the counting plate and reduce the error generated during cell counting.

[0005] Although the device has certain improvements, the upper counting plate and the lower counting plate in the device are fixedly connected and cannot be disassembled, so it is very troublesome to clean the cell fluid between the lower counting plate and the upper counting plate, which will take a long time. Utility Model Content

[0006] In order to make up for the above shortcomings, the utility model provides a cell counting plate, which aims to improve the problem in the prior art that the upper counting plate and the lower counting plate are fixedly connected and cannot be disassembled, and it is very troublesome and time-consuming to clean the cell fluid between the lower counting plate and the upper counting plate.

[0007] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: a cell counting plate, comprising a counting plate body, the lower surface of the counting plate body is provided with a buckle groove, the upper surface of the counting plate body is plugged with a lower counting plate, the upper surface of the counting plate body is plugged with an upper counting plate, the upper surface of the upper counting plate is penetrated and fixedly connected with an observation window, the upper surface of the lower counting plate is provided with a splitting component, the upper surface of the lower counting plate is provided with a sealing component, the upper surface of the upper counting plate is provided with a feeding component, the splitting component comprises a card slot, the inner wall of the card slot is plugged with a card block, the inner wall of the card block is elastically connected to a limiting block through a compression spring, and the inner wall of the lower counting plate is provided with a limiting slot.

[0008] As a further description of the above technical solution:

[0009] The disassembly assembly also includes a slide plate, the lower surface of which is fixedly connected to the rear end of the upper surface of the limit block, the slide plate penetrates and is slidably connected to the upper surface of the upper counting plate, the upper surface of the slide plate is fixedly connected to a connecting plate, and the upper surface of the connecting plate is fixedly connected to a pull ring.

[0010] As a further description of the above technical solution:

[0011] The sealing assembly comprises a rubber pad, a slide groove is provided on the upper surface of the lower counting plate, and the inner wall of the slide groove is elastically connected to a rubber plate via a connecting spring.

[0012] As a further description of the above technical solution:

[0013] The feeding assembly comprises a slot, an inner wall of the upper counting plate is provided with an annular groove, an insertion rod is inserted into the upper surface of the slot, and a rubber ring is fixedly connected to the middle section of the outer wall of the insertion rod.

[0014] As a further description of the above technical solution:

[0015] The card slot is opened at the right end of the upper surface of the lower counting board, the upper surface of the card block is fixedly connected to the right end of the lower surface of the upper counting board, the upper surface of the lower counting board and the lower surface of the upper counting board are fitted, and the card slot is connected to the limit groove.

[0016] As a further description of the above technical solution:

[0017] The front end of the lower surface of the limit block is opened as an inclined surface, the limit block passes through and is slidably connected to the front surface of the clamping block, the limit block is inserted into the inner wall of the limit groove, one end of the compression spring is fixedly connected to the rear surface of the limit block, and the other end of the compression spring is fixedly connected to the inner wall of the rear side of the clamping block.

[0018] As a further description of the above technical solution:

[0019] The upper surface of the rubber pad is fixedly connected to the lower surface of the upper counting plate, the rubber plate penetrates and is slidably connected to the upper surface of the slide groove, one end of the connecting spring is fixedly connected to the inner wall of the bottom end of the slide groove, the other end of the connecting spring is fixedly connected to the lower surface of the rubber plate, and the rubber pad is inserted into the inner wall of the slide groove.

[0020] As a further description of the above technical solution:

[0021] The slot is communicated with the annular groove, and the rubber ring is plugged into the inner wall of the annular groove.

[0022] The utility model has the following beneficial effects:

[0023] 1. In the utility model, the upper counting plate and the lower counting plate can be flexibly separated by the cooperation of the split components, so that the cell fluid inside them can be cleaned conveniently, thereby improving the cleaning efficiency of the counting plate, effectively reducing the cross contamination caused by cell residues, and ensuring the accuracy and reliability of subsequent cell counting experiments.

[0024] 2. In the present invention, by cooperating with the sealing component and the feeding component, different types of cell fluids can be placed in different counting slots for observation, which facilitates the observation of multiple cell fluids at the same time and enhances the practicability of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic front view of the three-dimensional structure of the overall device in the utility model;

[0026] Figure 2 It is a schematic diagram of the three-dimensional structure disassembly of the overall device in the utility model;

[0027] Figure 3 It is a schematic diagram of the three-dimensional structure section splitting of the lower counting board, the upper counting board and the card block in the utility model;

[0028] Figure 4 It is a schematic diagram of the three-dimensional structure of the lower counting plate, the upper counting plate, the rubber pad and the rubber plate of the utility model;

[0029] Figure 5 It is a schematic diagram of the three-dimensional structure of the upper counting plate and the insertion rod in the utility model.

[0030] Legend:

[0031] 1. Counting board body; 2. Buckle groove; 3. Lower counting board; 4. Upper counting board; 51. Block; 52. Compression spring; 53. Limit block; 54. Slide plate; 55. Connecting plate; 56. Pull ring; 501. Slot; 502. Limiting groove; 61. Rubber pad; 62. Rubber plate; 63. Connecting spring; 7. Observation window; 81. Insert rod; 82. Rubber ring; 801. Slot; 802. Annular groove. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0033] Reference Figure 1 - Figure 3 The utility model provides an embodiment: a cell counting board, comprising a counting board body 1, the left and right ends of the counting board body 1 are upwardly tilted, and the edges are provided with chamfers, the lower surface of the counting board body 1 is provided with buckle grooves 2, and there are two groups of buckle grooves 2, which are provided at the tilted parts of the left and right ends of the lower surface of the counting board body 1, and fingers can be inserted to facilitate gripping, so that the counting board body 1 is more convenient to transfer, the upper surface of the counting board body 1 is plugged with a lower counting board 3, the upper surface of the lower counting board 3 is divided into a plurality of counting grooves of the same area, and the cell fluid can be placed in the counting grooves for observation, the upper surface of the counting board body 1 is plugged with an upper counting board 4, the size of the upper counting board 4 is consistent with the shape of the lower counting board 3, when the upper counting board 4 and the lower counting board 3 and the counting board body 1 are plugged, the outer wall can fit with the counting board body 1, and the upper surface of the upper counting board 4 is penetrated and fixedly connected with an observation window 7, and the observation window 7 is provided in a circular shape to fit the objective lens of the microscope. The upper counting plate 4 and the lower counting plate 3 are combined, and the observation window 7 is made of glass and is transparent, which is convenient for people to observe cells using a microscope. The upper surface of the lower counting plate 3 is provided with a split component, which can quickly split the upper counting plate 4 and the lower counting plate 3, so as to facilitate the cleaning of the upper counting plate 4 and the lower counting plate 3. The upper surface of the lower counting plate 3 is provided with a sealing component, which can ensure that the upper counting plate 4 and the lower counting plate 3 can be sealed after installation. It not only maintains the seal between the two, but also ensures that the cell fluids in different counting grooves in the lower counting plate 3 do not interfere with each other, so as to facilitate the simultaneous observation of different types of cell fluids. The upper surface of the upper counting plate 4 is provided with a feeding component, which is convenient for adding cell fluids. The split component includes a card slot 501, and the inner wall of the card slot 501 is plugged with a card block 51. The inner wall of the card block 51 is elastically connected to the limit block 53 through a compression spring 52. The inner wall of the lower counting plate 3 is provided with a limit slot 502, and the limit block 53 and the limit slot 502 are matched.

[0034] Reference Figure 1 - Figure 3 The disassembly component also includes a slide plate 54, the lower surface of which is fixedly connected to the rear end of the upper surface of the limit block 53, the slide plate 54 and the limit block 53 will move synchronously, the slide plate 54 penetrates and is slidably connected to the upper surface of the upper counting plate 4, and slides in the front-to-back direction, the upper surface of the slide plate 54 is fixedly connected to a connecting plate 55, and the upper surface of the connecting plate 55 is fixedly connected to a pull ring 56, pulling the pull ring 56 upward will drive the connecting plate 55, the slide plate 54, and the limit block 53 to move upward, the limit block 53 will pull the upper counting plate 4 and the lower counting plate 3 to move upward, and the upper counting plate 4 and the lower counting plate 3 will be separated from the counting plate body 1, if the pull ring 56 is pulled backward first, the limit block 53 can be moved backward and retracted into the card block 51, and then the pull ring 56 is pulled upward to separate the upper counting plate 4 and the lower counting plate 3.

[0035] Reference Figure 1 , Figure 3 , Figure 4 The sealing assembly includes a rubber pad 61, which is a combination of horizontal and vertical rubber strips and is an integrally molded casting. A slide groove is provided on the upper surface of the lower counting plate 3, and the inner wall of the slide groove is elastically connected to a rubber plate 62 through a connecting spring 63. The rubber plate 62 and the rubber pad 61 have the same shape.

[0036] Reference Figure 1 , Figure 5 The feed assembly includes a slot 801, an annular groove 802 is formed on the inner wall of the upper counting plate 4, an insertion rod 81 is inserted into the upper surface of the slot 801, and multiple groups of the insertion rods 81 are evenly distributed above each counting slot, so as to facilitate the addition of different types of cell fluids into different counting slots, and a rubber ring 82 is fixedly connected to the middle section of the outer wall of the insertion rod 81.

[0037] Reference Figure 1 - Figure 3 The card slot 501 is opened at the right end of the upper surface of the lower counting board 3. There are two groups of split components, which are evenly distributed at the left and right ends of the lower counting board 3. The upper surface of the card block 51 is fixedly connected to the right end of the lower surface of the upper counting board 4. The upper surface of the lower counting board 3 and the lower surface of the upper counting board 4 fit together to maintain sealing. The card slot 501 is connected to the limit groove 502.

[0038] Reference Figure 1 - Figure 3The front end of the lower surface of the limit block 53 is opened as an inclined surface. By squeezing the inclined surface of the limit block 53, the limit block 53 will move backward and retract into the card block 51 to release the obstruction. The limit block 53 passes through and is slidably connected to the front surface of the card block 51. The limit block 53 is inserted into the inner wall of the limit groove 502, and the card block 51 can be limited. One end of the compression spring 52 is fixedly connected to the rear surface of the limit block 53, and the other end of the compression spring 52 is fixedly connected to the inner wall of the rear side of the card block 51. When the limit block 53 moves backward, it will squeeze the compression spring 52 to generate a reaction force.

[0039] Reference Figure 1 , Figure 3 , Figure 4 The upper surface of the rubber pad 61 is fixedly connected to the lower surface of the upper counting plate 4, the rubber plate 62 penetrates and is slidably connected to the upper surface of the slide groove, and slides longitudinally. One end of the connecting spring 63 is fixedly connected to the inner wall of the bottom end of the slide groove, and the other end of the connecting spring 63 is fixedly connected to the lower surface of the rubber plate 62. When the rubber plate 62 moves downward, it will squeeze the connecting spring 63 to generate a reaction force. The rubber pad 61 is inserted into the inner wall of the slide groove. When inserted, the rubber pad 61 will squeeze the upper surface of the rubber plate 62. The two will be tightly attached to maintain the seal under the influence of the reaction force of the connecting spring 63.

[0040] Reference Figure 1 , Figure 5 The slot 801 is connected to the annular groove 802, the rubber ring 82 is inserted into the inner wall of the annular groove 802, the slot 801 is slightly larger than the insertion rod 81, and when the insertion rod 81 and the slot 801 are inserted, the rubber ring 82 will be squeezed and deformed.

[0041] Working principle: When in use, first pull out the rod 81 from the slot 801, and inject the cell fluid from the slot 801 into the counting groove on the lower counting plate 3. After the cell fluid is injected, plug the rod 81 and the slot 801. During the plugging, the rubber ring 82 will be squeezed and deformed. After the plugging, the rubber ring 82 will be aligned with the annular groove 802. Under the influence of the elasticity of the rubber ring 82 itself, the rubber ring 82 will be plugged into the annular groove 802. Without the influence of external force, the rubber ring 82 cannot be separated from the annular groove 802. At this time, the concentration of the cell fluid in different counting grooves can be observed at the observation window 7.

[0042] When the device needs to be moved as a whole, just insert your finger into the buckle slot 2 to move the counting board body 1.

[0043] In the normal state of the disassembly assembly, the compression spring 52 pushes the limit block 53 to be inserted into the limit groove 502, so that the upper counting board 4 and the lower counting board 3 are stably connected. When it is necessary to disassemble, the pull ring 56 is pulled upward to drive the connecting plate 55, the slide plate 54, and the limit block 53 to move upward, so that the upper counting board 4 and the lower counting board 3 are separated from the counting board body 1. Then, pull the pull ring 56 backward to make the limit block 53 move backward and retract into the card block 51, release the limit on the card block 51, and then pull the pull ring 56 upward to realize the separation of the upper counting board 4 and the lower counting board 3, which is convenient for cleaning. When installation is required, align the card block 51 and the card slot 501 and plug them in. During plugging, the inclined surface of the limit block 53 will be squeezed, and the limit block 53 will move backward and retract into the card block 51 to release the obstruction, and will squeeze the compression spring 52 to generate a reaction force. When the plugging is completed, the compression spring 52 will push the limit block 53 to move, so that the limit block 53 and the limit slot 502 are plugged in to limit the card block 51, and then the assembled upper counting board 4 and lower counting board 3 are plugged into the counting board body 1.

[0044] During installation, the rubber pad 61 will be inserted into the slide groove and squeeze the rubber plate 62. Under the reaction force of the connecting spring 63, the rubber pad 61 and the rubber plate 62 fit tightly, which not only maintains the seal between the upper counting plate 4 and the lower counting plate 3, but also ensures that the cell fluids in different counting grooves in the lower counting plate 3 do not interfere with each other.

[0045] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A cell counting chamber, comprising a counting chamber body (1), characterized in that: The lower surface of the counting plate body (1) is provided with a buckle groove (2), the upper surface of the counting plate body (1) is plugged with a lower counting plate (3), the upper surface of the counting plate body (1) is plugged with an upper counting plate (4), the upper surface of the upper counting plate (4) is penetrated by and fixedly connected with an observation window (7), the upper surface of the lower counting plate (3) is provided with a splitting component, the upper surface of the lower counting plate (3) is provided with a sealing component, the upper surface of the upper counting plate (4) is provided with a feeding component, the splitting component comprises a card slot (501), the inner wall of the card slot (501) is plugged with a card block (51), the inner wall of the card block (51) is elastically connected to a limit block (53) through a compression spring (52), and the inner wall of the lower counting plate (3) is provided with a limit slot (502).

2. The cell counting chamber according to claim 1, characterized in that: The disassembly assembly further comprises a slide plate (54), the lower surface of the slide plate (54) being fixedly connected to the rear end of the upper surface of the limit block (53), the slide plate (54) passing through and slidably connected to the upper surface of the upper counting plate (4), the upper surface of the slide plate (54) being fixedly connected to a connecting plate (55), and the upper surface of the connecting plate (55) being fixedly connected to a pull ring (56).

3. The cell counting chamber according to claim 1, characterized in that: The sealing assembly comprises a rubber pad (61), the upper surface of the lower counting plate (3) is provided with a slide groove, and the inner wall of the slide groove is elastically connected to a rubber plate (62) via a connecting spring (63).

4. The cell counting chamber according to claim 1, characterized in that: The feeding assembly comprises a slot (801), an inner wall of the upper counting plate (4) is provided with an annular groove (802), an insertion rod (81) is inserted into the upper surface of the slot (801), and a rubber ring (82) is fixedly connected to the middle section of the outer wall of the insertion rod (81).

5. The cell counting chamber according to claim 1, characterized in that: The card slot (501) is provided at the right end of the upper surface of the lower counting plate (3), the upper surface of the card block (51) is fixedly connected to the right end of the lower surface of the upper counting plate (4), the upper surface of the lower counting plate (3) and the lower surface of the upper counting plate (4) are in contact with each other, and the card slot (501) and the limit slot (502) are in communication.

6. The cell counting chamber according to claim 1, characterized in that: The front end of the lower surface of the limit block (53) is provided with an inclined surface, the limit block (53) penetrates through and is slidably connected to the front surface of the clamping block (51), the limit block (53) is inserted into the inner wall of the limit groove (502), one end of the compression spring (52) is fixedly connected to the rear surface of the limit block (53), and the other end of the compression spring (52) is fixedly connected to the inner wall of the rear side of the clamping block (51).

7. The cell counting chamber according to claim 3, characterized in that: The upper surface of the rubber pad (61) is fixedly connected to the lower surface of the upper counting plate (4), the rubber plate (62) penetrates and is slidably connected to the upper surface of the slide groove, one end of the connecting spring (63) is fixedly connected to the inner wall of the bottom end of the slide groove, the other end of the connecting spring (63) is fixedly connected to the lower surface of the rubber plate (62), and the rubber pad (61) is inserted into the inner wall of the slide groove.

8. The cell counting chamber according to claim 4, characterized in that: The slot (801) is connected to the annular groove (802), and the rubber ring (82) is inserted into the inner wall of the annular groove (802).

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