Gene amplification instrument

Abstract

The utility model relates to a gene amplification instrument which comprises an equipment body, a heat cover and a temperature control assembly, the equipment body is provided with a heating block used for placing a plurality of reagent tubes, the temperature control assembly is arranged in the equipment body, the heat cover is rotationally connected to the equipment body, and the temperature control assembly is arranged in the equipment body. And the heat cover is used for abutting against the end surfaces of a plurality of reagent tubes. The method has the effect of improving the temperature control precision of the gene amplification instrument.

Description

Technical Field

[0001] The present application relates to the field of laboratory equipment, and in particular to a gene amplification instrument. Background Art

[0002] Gene amplification instruments are mainly used for gene amplification in scientific research and clinical practice, stereotyped PCR gene amplification, fluorescence / enzyme immunoassay endpoint DNA gene amplification, gene chips and other gene amplification applications.

[0003] At present, the Chinese utility model patent with publication number CN221016130U discloses a small constant temperature metal bath for laboratory use, including a metal bath body, a metal block is arranged on the top of the metal bath body, and slots are opened on the left and right sides of the top of the metal bath body; a lifting mechanism, the lifting mechanism includes a round block, a round rod is fixedly connected to the top of the round block, and a movable plate is fixedly connected to the top of the round rod. The utility model is convenient for the staff to take out the reagent samples at one time, reducing the difference in the heating time of the samples.

[0004] The reagent tube is always in an open state during the heating process of the gene thermal cycler. The heat in the reagent tube will escape, causing the temperature fluctuation of the reagent tube to increase and the temperature control accuracy of the gene thermal cycler to be low. Utility Model Content

[0005] In order to improve the temperature control accuracy of a gene amplification instrument, the present application provides a gene amplification instrument.

[0006] The present application provides a gene amplification instrument, which adopts the following technical solution:

[0007] A gene amplification instrument comprises an equipment body, a heat cover and a temperature control component. The equipment body is provided with a heating block for placing a plurality of reagent tubes, the temperature control component is arranged in the equipment body, the heat cover is rotatably connected to the equipment body, and the heat cover abuts against the end faces of a plurality of reagent tubes.

[0008] By adopting the above technical solution, the staff first places the reagent tube to be tested on the heating block, and then flips the heat cover and places the heat cover against the end face of the reagent tube, and then uses the temperature control component to adjust the temperature of the heating block to achieve temperature control of the reagent tube. The heat cover reduces heat escape from the reagent tube, reduces temperature fluctuations of the reagent tube, and improves the temperature control accuracy of the gene amplification instrument.

[0009] Optionally, a locking piece for keeping the hot cover in contact with the end face of the reagent tube is provided on the device body, the locking piece comprises a pushing block, a matching block, a locking block and a locking spring, a locking groove is provided on the hot cover, the matching block is slidably connected to the device body in a direction approaching or away from the hot cover, the locking block is provided on the matching block, the locking spring is used to keep the locking block engaged in the locking groove, the pushing block is slidably connected to the device body, and the matching block is provided with a guiding inclined surface for facilitating the pushing block to drive the matching block to move in a direction away from the locking groove.

[0010] By adopting the above technical solution, the locking piece can ensure that the hot cover can always abut against the end face of the reagent tube. The staff first places the reagent tube on the heating block, and the staff can push the push block, which abuts against the guiding inclined surface of the matching block. The matching block overcomes the elastic force of the locking spring and moves in the direction away from the locking groove. Then the staff rotates the hot cover, and the hot cover abuts against the end face of the reagent tube. The locking groove of the hot cover is aligned with the locking block. Then the staff releases the push block, and the locking spring drives the matching block to move. The matching block drives the locking block to be clamped into the locking groove, so that the hot plate cannot rotate, and the hot plate remains abutted against the end face of the reagent tube.

[0011] Optionally, the hot cover includes a cover body, a heating plate and a plurality of abutment springs, wherein the heating plate is slidably connected to the cover body along the thickness direction of the cover body, and the plurality of abutment springs are evenly distributed on the cover body, and the abutment springs are used to maintain a distance between the cover body and the heating plate, and the heating plate is used to abut against the end face of the reagent tube.

[0012] By adopting the above technical solution, due to the different capacities of the reagent tubes, resulting in different lengths of the reagent tubes, the abutment spring can drive the heating plate to move along the thickness direction of the cover body, the cover body is locked on the device body by a locking piece, and the heating plate can always be close to the reagent tube, and the hot cover can be suitable for reagent tubes of different lengths.

[0013] Optionally, the thermal cover further comprises a torsion spring, and the torsion spring is used for rotating the cover body in a direction away from the device body.

[0014] By adopting the above technical solution, when the reagent tube needs to be taken out from the heating block after completing the experiment, the staff can press the push block, and the push block can drive the locking block to compress the locking spring through the guiding inclined surface, so that the locking block disengages from the locking groove. At this time, the torsion spring can drive the cover body to rotate in the direction away from the heating block. There is no need for the staff to manually open the hot cover, which is convenient for the staff to operate with one hand, thereby improving the convenience of the equipment.

[0015] Optionally, the temperature control component includes a plurality of Peltiers, a heat sink and a fan, the plurality of Peltiers are evenly distributed on the lower end surface of the heating block, the heat sink is arranged at one end of the Peltier away from the heating block, and the fan is arranged on one end of the heat sink away from the Peltier.

[0016] By adopting the above technical solution, Peltier is a semiconductor component that can both generate heat and cool. Peltier can be used to heat and cool the heating rack to control the temperature of the reagent tube. Due to the characteristics of Peltier, when one end of the Peltier absorbs heat from the heating block, the other end of the Peltier will release heat, and this heat will affect the inside of the device. The heat at the heat-releasing end of the Peltier can be discharged from the device body as soon as possible through the heat sink and the fan.

[0017] Optionally, the temperature control component further includes an auxiliary heating film, and the auxiliary heating film is arranged on the heating block.

[0018] By adopting the above technical solution, when the Peltier device heats the heating block, the temperature around the heating block will be lower than the temperature in the middle of the heating block. The auxiliary heating film heats the surrounding side of the heating block, the temperature of the heating block is more uniform, and the temperature difference between several reagent tubes is reduced.

[0019] Optionally, ventilation holes are provided on the device body.

[0020] By adopting the above technical solution, the ventilation holes are used to discharge the heat generated by the temperature control component to the heat sink out of the device body, thereby extending the service life of the device body.

[0021] Optionally, the device body is provided with horizontal and vertical markings for the staff to identify the reagent tubes.

[0022] By adopting the above technical solution, horizontal marking and vertical marking make it easier for staff to accurately find the required reagent tubes, thereby improving work efficiency.

[0023] In summary, the present application includes at least one of the following beneficial technical effects:

[0024] 1. The hot cover reduces the heat escape of the reagent tube during the heating process and improves the temperature control accuracy of the equipment;

[0025] 2. The locking piece ensures that the thermal cover always abuts against the end surface of the reagent tube, thus improving the reliability of the thermal cover;

[0026] 3. The temperature control component makes it easy for staff to heat or cool the reagent tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 This is a schematic diagram of a gene amplification instrument.

[0028] Figure 2 yes Figure 1 Exploded view of the temperature control components inside the middle shell.

[0029] Figure 3 yes Figure 1 Cross-sectional view of the mid-heat cover.

[0030] Figure 4 yes Figure 1 A cross-sectional view of the middle housing is used to illustrate the structure of the locking member.

[0031] Figure numerals: 1. Equipment body; 11. Shell; 111. Through groove; 112. Ventilation hole; 12. Base; 2. Heat cover; 21. Cover; 211. Receiving groove; 212. Locking groove; 22. Heating plate; 23. Abutment spring; 24. Torsion spring; 3. Temperature control component; 31. Peltier; 32. Heat sink; 33. Fan; 34. Auxiliary heating film; 4. Heating block; 41. Placement tube; 5. Locking piece; 51. Push block; 52. Matching block; 521. Guide ramp; 53. Locking block; 531. Guide ramp; 54. Locking spring; 55. Matching rod. DETAILED DESCRIPTION

[0032] The following is combined with Figure 1-4 This application is described in further detail.

[0033] The present application embodiment discloses a gene amplification instrument. Figure 1 and Figure 2 A gene amplification instrument includes an equipment body 1, a heat cover 2 and a temperature control component 3. The equipment body 1 includes a shell 11 and a base 12. The shell 11 is arranged on the base 12. A through groove 111 is provided on the upper end surface of the shell 11. A heating block 4 is fixedly arranged in the through groove 111. The heating block 4 is provided with a plurality of placement tubes 41 for placing reagent tubes. The heat cover 2 is rotatably connected to the shell 11, and the heat cover 2 is used to abut against the end surface of the reagent tube.

[0034] Reference Figure 1 and Figure 2The temperature control component 3 includes six Peltiers 31, a heat sink 32, a fan 33 and an auxiliary heating film 34. The Peltier 31 is horizontally arranged, the fan 33 is located below the heating block 4, the fan 33 is fixedly arranged on the upper end surface of the base 12, the heat sink 32 is fixedly arranged on the upper end surface of the fan 33, the lower end surface of the Peltier 31 is fixedly arranged on the upper end surface of the heat sink 32, the lower end surface of the Peltier 31 is fixedly arranged on the lower end surface of the heating block 4, the six Peltiers 31 are evenly distributed and adjacent Peltiers 31 abut against each other, the auxiliary heating film 34 is horizontally arranged, the auxiliary heating film 34 is annular, the auxiliary heating film 34 surrounds a plurality of placement tubes 41, and the auxiliary heating film 34 is fixedly arranged on the upper end surface of the heating block 4. A plurality of ventilation holes 112 are opened on the outer wall of the shell 11, and a transverse mark and a longitudinal mark are arranged on the upper end surface of the shell 11, and the transverse mark and the longitudinal mark are both located on the peripheral side of the through groove 111.

[0035] Reference Figure 1 and Figure 3 The hot cover 2 includes a cover body 21, a heating plate 22, a plurality of abutting springs 23 and two torsion springs 24. The cover body 21 is rotatably connected to the shell body 11 along the horizontal direction. A receiving groove 211 is provided inside the cover body 21. The receiving groove 211 extends along the thickness direction of the cover body 21. The heating plate 22 is slidably connected in the receiving groove 211 along the extension direction of the receiving groove 211. A plurality of abutting springs 23 are evenly distributed at the bottom of the receiving groove 211. The length direction of the abutting spring 23 is parallel to the sliding direction of the heating plate 22. One end of the abutting spring 23 is fixedly arranged at one end of the heating plate 22 facing the bottom of the receiving groove 211, and the other end of the abutting spring 23 is fixedly arranged at the bottom of the receiving groove 211. The two torsion springs 24 are distributed along the rotation axis direction of the cover body 21. The torsion spring 24 is sleeved on the rotation axis of the cover body 21. One end of the torsion spring 24 is fixedly arranged on the shell, and the other end of the torsion spring 24 is fixedly arranged on the cover body 21.

[0036] Reference Figure 1 and Figure 4The housing 11 is also provided with a locking member 5 for keeping the heating plate 22 in contact with the end surface of the reagent tube. The locking member 5 includes a push block 51, a matching block 52, a locking block 53, two locking springs 54 and two matching rods 55. The matching block 52 is slidably connected to the housing 11 along the length direction of the housing 11. The locking block 53 is fixedly arranged on the side wall of the matching block 52 facing the cover body 21. A guiding inclined surface 521 is provided between the upper end surface of the matching block 52 and the side wall of the locking block 53 facing the cover body 21. A guiding inclined surface 531 is provided between the upper end surface of the locking block 53 and the side wall of the locking block 53 facing the cover body 21. The two locking springs 54 are arranged along the cover body 21 is distributed in the width direction, the length direction of the locking spring 54 is parallel to the sliding direction of the locking block 53, one end of the locking spring 54 is fixedly set on the matching block 52, and the other end of the locking spring 54 is fixedly set on the shell body 11, and the push block 51 is slidably connected to the upper end surface of the shell body 11 in the vertical direction, and the two matching rods 55 are distributed along the width direction of the shell body 11. The matching rod 55 is vertically set, and the upper end surface of the matching rod 55 is fixedly set on the push block 51, and the lower end surface of the matching rod 55 abuts on the guide inclined surface 521. The lower end surface of the matching rod 55 is arc-shaped, and a locking groove 212 for clamping the locking block 53 is provided on the cover body 21.

[0037] The implementation principle of a gene amplification instrument in an embodiment of the present application is as follows: the staff first inserts the reagent tube into the placement tube 41, and then the staff rotates the cover body 21 toward the locking piece 5 to overcome the elastic force of the torsion spring 24. When the cover body 21 rotates, it abuts against the guide slope 531, and the guide slope 531 drives the matching block 52 and the locking block 53 to move away from the cover body 21 until the locking groove 212 is aligned with the locking block 53. The locking block 53 enters the locking groove 212 under the action of the locking spring 54, and the heating plate 22 remains abutted against the upper end surface of the reagent tube. Through the abutment spring 23, the heating plate 22 can adapt to reagent tubes of different lengths.

[0038] After completing the installation of the heat cover 2, the staff can power on the Peltier 31 to adjust the temperature of the reagent tube. When the reagent tube needs to be cooled, the lower end surface of the Peltier 31 will be heated, and the heat on the Peltier 31 will be discharged through the heat sink 32 and the fan 33. The heat can be discharged from the ventilation hole 112 and the lower end surface of the base 12.

[0039] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. A gene amplification instrument, characterized in that: The device comprises a device body (1), a heat cover (2) and a temperature control component (3); the device body (1) is provided with a heating block (4) for placing a plurality of reagent tubes; the temperature control component (3) is arranged in the device body (1); the heat cover (2) is rotatably connected to the device body (1); the heat cover (2) is used to abut against the end faces of the plurality of reagent tubes; the device body (1) is provided with a locking member (5) for keeping the heat cover (2) abutting against the end faces of the reagent tubes; the locking member (5) comprises a pushing block (51), a matching block (52), a locking block (53) and a locking spring (54); The heat cover (2) is provided with a locking groove (212); the matching block (52) is slidably connected to the device body (1) in a direction approaching or moving away from the heat cover (2); the locking block (53) is arranged on the matching block (52); the locking spring (54) is used to keep the locking block (53) engaged in the locking groove (212); the push block (51) is slidably connected to the device body (1); and the matching block (52) is provided with a guiding inclined surface (521) for facilitating the push block (51) to drive the matching block (52) to move in a direction away from the locking groove (212).

2. A gene amplification instrument according to claim 1, characterized in that: The thermal cover (2) comprises a cover body (21), a heating plate (22) and a plurality of abutment springs (23); the heating plate (22) is slidably connected to the cover body (21) along the thickness direction of the cover body (21); the plurality of abutment springs (23) are evenly distributed on the cover body (21); the abutment springs (23) are used to keep a distance between the cover body (21) and the heating plate (22); and the heating plate (22) is used to abut against the end surface of a reagent tube.

3. A gene amplification instrument according to claim 2, characterized in that: The thermal cover (2) further comprises a torsion spring (24), wherein the torsion spring (24) is used to rotate the cover body (21) in a direction away from the device body (1).

4. A gene amplification instrument according to claim 1, characterized in that: The temperature control component (3) comprises a plurality of Peltiers (31), a heat sink (32) and a fan (33); the plurality of Peltiers (31) are evenly distributed on the lower end surface of the heating block (4); the heat sink (32) is arranged at one end of the Peltier (31) facing away from the heating block (4); and the fan (33) is arranged at one end of the heat sink (32) facing away from the Peltier (31).

5. A gene amplification instrument according to claim 4, characterized in that: The temperature control component (3) further comprises an auxiliary heating film (34), wherein the auxiliary heating film (34) is arranged on the heating block (4).

6. A gene amplification instrument according to claim 1, characterized in that: A ventilation hole (112) is provided on the device body (1).

7. A gene amplification instrument according to claim 1, characterized in that: The device body (1) is provided with a transverse mark and a longitudinal mark for facilitating staff to identify the reagent tube.

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